Different genes gene expression profiling. Medical search. Frequent questions

The results provide evidence that genes involved in tissue remodeling and repair are differentially regulated in the lungs of obstructed smokers and suggest that they are potential therapeutic targets. (nih.gov)
Predictions achieve correlations of 0.68 with true values, and maintain accurate differentially expressed genes (AUC 0.81). (nih.gov)
This study was aimed at determining the differential gene expression patterns of OBP genes in the developmental stages (larval, pupal, teneral) and differentially starved adults of Glossina brevipalpis (Newstead) collected from Shimba Hills National Park, Kenya. (bioone.org)
Hypergeometric analysis was used to identify canonical pathways and curated networks having statistically significant enrichment of differentially expressed genes. (urotoday.com)
The two expression profile datasets were integrated and we found out coexpression of differentially expressed genes (DEGs) using bioinformatics methods. (hindawi.com)
With the development of the gene expression profile, comparisons of differentially expressed genes that participate in the regulation of pathophysiological conditions in pathological tissue and normal tissue were available and convenient, and mRNAs gene expression profiles have been carried out in many diseases including TAD [ 6 ]. (hindawi.com)
Then we downloaded another microarray dataset GSE52093 [ 9 ] and screened out the coexpression of differentially expressed genes (DEGs) between TAD patients and non-TAD patients. (hindawi.com)
The peroxisome proliferator- activated receptor gamma coactivator 1-alpha ( Ppargc1a ) gene was found to be involved in the networks generated using Ingenuity Pathway Studio (IPA) from the differentially expressed transcript cluster lists of week 2 versus 1, week 3 versus 2, week 6 versus 3, and week 8 versus 6. (molvis.org)
The purpose of the study was to identify the key genes that were differentially expressed in these disc cells compared to cells without inflammatory cytokine treatment, using bioinformatics analyses, and to explore the related signaling pathways and interaction networks, providing clues to the molecular mechanisms of disc degeneration for future experimental studies. (minervamedica.it)
4 samples each) were identified using the R language, and were pooled using the Excel software program to select the common differentially expressed genes in the two datasets. (minervamedica.it)
RESULTS: Of the 255 concomitantly and differentially expressed genes identified after respective treatment with TNF-α and IL-1β, 141 were up-regulated and 114 were down-regulated. (minervamedica.it)
The gene ontology annotation analysis showed that these differentially expressed genes were primarily associated with cytokine activity, growth factor activity, the inflammatory reaction and the response to injury. (minervamedica.it)
The signaling pathway analysis showed that these differentially expressed genes were mainly related to the interactions of cytokines, apoptosis and NOD-like receptor signaling pathways. (minervamedica.it)
However, between malaria infected and coinfected children there were 240 differentially expressed genes with 180 genes upregulated and 60 downregulated in the coinfected group. (biomedcentral.com)
Using a false discovery rate of 0.05, a total of 1,497 differentially expressed genes were identified. (biomedcentral.com)
The mHTT and wtHTT groups showed the highest number of differentially expressed genes compared to the BACHD mouse model. (lu.se)
In BACHD mice, few hypothalamic genes were differentially expressed compared to age-matched WT controls. (lu.se)
Between late pregnancy (135 days of gestation ± 2.4 SD) and lactation (15 days post partum ± 1.27 SD) 13 % of genes in the sheep genome were differentially expressed in the ovine mammary gland . (bvsalud.org)

Notably, a large number of stress-related elements present in the promoter regions of some ZmCAMTA genes, indicating a genetic basis of stress expression regulation of these genes. (frontiersin.org)
CAMTAs participate in gene expression regulation by binding to the cis -elements in the promoter regions of numerous target genes. (frontiersin.org)
Furthermore, functions assigned to mouse and human RPE expression profiles indicate (patho-) biological differences related to AMD, such as oxidative stress, Bruch's membrane, immune-regulation and outer blood retina barrier. (vu.nl)
Coordinated transcriptional regulation of large numbers of genes is required to switch between mammary tissue establishment during late pregnancy , and activation and maintenance of milk production during lactation . (bvsalud.org)
Our findings indicate the remarkable plasticity of the mammary gland , and the coordinated regulation of multiple genes and pathways to begin milk production . (bvsalud.org)

We analyse two comprehensive transcriptome datasets from human tissues and human-derived cell lines in terms of the expression profiles of the SLC and ABC families of membrane transporters. (biorxiv.org)
In conclusion, we show that both wtHTT and mHTT overexpression change hypothalamic transcriptome profile, specifically mHTT, altering neuroendocrine circuits. (lu.se)

At recruitment, whole blood was collected into Paxgene tubes from a subset of children for transcriptional profiling among uninfected (n = 12), S. haematobium infected (n = 16), malaria infected (asymptomatic, n = 8) or coinfected (n = 13) children. (biomedcentral.com)
In this study, we investigated whether hypothalamic HTT expression causes transcriptional changes. (lu.se)
Changes in the expression of epigenetic regulators, particularly chromatin remodellers, indicate a possible role in coordinating the large-scale transcriptional changes that appear to be required to switch mammary processes from growth and development during late pregnancy to synthesis and secretion of milk during lactation . (bvsalud.org)
We have used transcript profiling to explore the early transcriptional responses of mature C. albicans biofilms exposed to various antifungal agents. (who.int)

Genes contain the instructions for making messenger RNA (mRNA), but at any moment each cell makes mRNA from only a fraction of the genes it carries. (wikipedia.org)
Expression profiling experiments often involve measuring the relative amount of mRNA expressed in two or more experimental conditions. (wikipedia.org)
These profiles measure mRNA, not protein, so further studies are needed to corroborate that any detected changes in gene expression actually have meaning in the life of the cell. (genengnews.com)
Using mRNA from total blood leukocytes, they found that a full one-quarter of the human genome exhibits changes in expression levels during the early stages of post-trauma (40-80 hours post-trauma). (genengnews.com)
mRNA from the specimens was profiled using Agilent's Functional ID v2.0 array (Agilent, Santa Clara, CA) containing 23,720 sequences. (nih.gov)
In the present study, we use mRNA microarray to acquire differential expression profiles in human TAD tissues and non-TAD tissues. (hindawi.com)
In general, mRNA levels of ECM and collagen genes have been found to be downregulated in both the adult mouse sclera [ 6 ] and cornea [ 7 ] when compared with samples from an earlier developmental stage. (molvis.org)
For the first time we described the kinetics of hepatic ontogenesis at mRNA level in mule ducks and highlighted different expression patterns depending on the cellular pathway. (biomedcentral.com)
As expected, the SCN showed high expression of arginine vasopressin, vasoactive intestinal polypeptide, calbindin and nuclear receptor subfamily 1, group D, member 1 (NR1D1) (also known as reverse strand of ERBA (REV-ERBα), both at the mRNA and protein level. (elsevierpure.com)

The human genome contains on the order of 20,000 genes which work in concert to produce roughly 1,000,000 distinct proteins. (wikipedia.org)
This is due to alternative splicing, and also because cells make important changes to proteins through posttranslational modification after they first construct them, so a given gene serves as the basis for many possible versions of a particular protein. (wikipedia.org)
While knowledge of the precise proteins a cell makes (proteomics) is more relevant than knowing how much messenger RNA is made from each gene,[why? (wikipedia.org)
Odorant binding proteins (OBPs) are involved in either olfactory or nonolfactory cue sensation depending on their spatial and temporal expression. (bioone.org)
The SEPTIN9 gene provides instructions for making a protein called septin-9, which is part of a group of proteins called septins. (medlineplus.gov)

Five OBP genes, GbrOBP2 , GbrOBP6 , GbrOBP7 , GbrOBP8 , and GbrOBP13 , were selected for quantitative PCR expression analysis as representatives of the three OBP classes in Glossina spp. (bioone.org)
Quantitative real-time PCR was used to test the expression of ZmCAMTA genes under several abiotic stresses (drought, salt, and cold), various stress-related hormones [abscisic acid, auxin, salicylic acid (SA), and jasmonic acid] and biotic stress [rice black-streaked dwarf virus (RBSDV) infection]. (frontiersin.org)
QIAseq Targeted RNA Panels have been developed as a Sample to Insight solution for quantitative gene expression profiling using RNAseq. (qiagen.com)
To test this hypothesis, we used gene arrays and quantitative RT-PCR to profile age-related gene expression changes within the SCN of male rhesus macaques - a pragmatic translational animal model of human aging, which similarly displays an age-related attenuation of daytime activity levels. (elsevierpure.com)

Genes in this pathway have been shown to be involved in the activation of transforming growth factor (TGF)-beta1 and matrix metalloproteinases and are subject to inhibition by SERPINE2. (nih.gov)
Genes associated with the classical complement pathway were downregulated in non-muscle invasive cancer. (urotoday.com)
The gene ontology and pathway enrichment of DEGs were performed by DAVID and Kyoto Encyclopedia of Genes and Genomes online analyses, respectively. (hindawi.com)
Traditional bioinformatics methods including gene ontology (GO) and pathways enrichment analyses of DEGs combined protein-protein interaction (PPI) network and cytohubber calculating software as well as Molecular Complex Detection (MCODE) analysis were used to analyze the key gene and pathway. (hindawi.com)
Pathway analysis revealed substantial modulation of gene expression in defence and immune responses and in particular modulation of genes in the Fcγ-receptor-mediated phagocytosis pathway. (biomedcentral.com)
Since liver sampling was only possible from the 12th day of embryogenesis (E12), we analyzed hepatic gene expression at 7 sampling points every 4 days from this point up to 4 days post-hatch (D4) and revealed different patterns of expression depending on the cellular pathway. (biomedcentral.com)
Can testing of tumor tissue for mutations in EGFR pathway downstream effector genes. (cdc.gov)

Characterisation of gene expression patterns in bladder cancer allows the identification of pathways involved in its pathogenesis, and may stimulate the development of novel therapies targeting these pathways. (urotoday.com)
The results showed that nine ZmCAMTA genes showed highly diversified gene structures and tissue-specific expression patterns. (frontiersin.org)
However, little information is available on the gene expression profile (GEP) of CMV-CTL clones according to CMV reactivation patterns (and donor CMV serostatus) after allo-HCT. (nature.com)
Cluster analysis revealed 2 groups of patients with insulinoma and with distinct patterns of gene expression. (elsevierpure.com)
To analyze microarray datasets (34760 variables) and obtain functional implications of differential expression patterns, we used Linear Models for Microarray Data (limma) followed by Gene Set Enrichment Analysis (GSEA) using ClusterProfiler. (lu.se)

The results of these studies have been used to investigate disease mechanisms, monitor progression of disease and identify specific genes or gene signatures which could be used as diagnostic or prognostic biomarkers. (videolectures.net)
Specific genes of relevance to malaria infection, including CD8a, were significantly downregulated in malaria-infected compared to coinfected children. (biomedcentral.com)
The goal of our search was to identify publications describing the role of specific genes, gene signatures, or molecular pathways and their effects in response to environmental exposures. (cdc.gov)
In particular, exposure to CAS resulted in the upregulation of hypha-specific genes known to play a role in biofilm formation, such as ALS3 and HWP1. (who.int)

Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell. (wikipedia.org)
Expression profiling is a logical next step after sequencing a genome: the sequence tells us what the cell could possibly do, while the expression profile tells us what it is actually doing at a point in time. (wikipedia.org)
In this study, we first identified all the CAMTA homologous genes in the whole genome of maize. (frontiersin.org)
Overall, our analyses provide novel opportunities for the normalisation of genome-wide expression profiling data. (biorxiv.org)
Given that the basic genome of a differentiated organism is constant between cells (and we here ignore epigenomics), what mainly discriminates one cell type from another is its expression profile. (biorxiv.org)
More than half (23 of 39) of the articles represent epidemiologic studies including candidate gene and genome-wide association studies (GWAS) examining genetic effect modification on disease risk. (cdc.gov)

6 ], the transforming growth factor beta 1 ( Tgfβ1 ) gene and several collagen genes were significantly downregulated in 8-week-old B6 mice scleras when compared with 3-week-old B6 mice scleras. (molvis.org)

Hara Levy, professor of physiology at the Human and Molecular Genetics Center of the Medical College of Wisconsin, studies cystic fibrosis (CF). Although CF is caused by mutations in the CF transmembrane conductance regulator gene, patients with identical genetic mutations in this gene can suffer variable levels of lung disease severity. (genengnews.com)
Interestingly, both TGF-beta1 and SERPINE2 have been identified as candidate genes in COPD genetic linkage and association studies. (nih.gov)
Duplication of genetic material within the SEPTIN9 gene has also been identified in affected individuals. (medlineplus.gov)

At the "Critical Assessment of Massive Data Analysis" conference held recently in Vienna, John Storey, from the Lewis-Sigler Institute for Integrative Genomics at Princeton University, gave a talk on dissecting the inflammatory complications in critically injured patients by within-patient gene-expression changes. (genengnews.com)
To explore work on gene-environment interaction (GxE) at the Centers for Disease Control and Prevention (CDC) and the Agency for Toxic Substances and Disease Registry (ATSDR), we examined the CDC-Authored Genomics and Precision Health Publications Database (CDC-Authored GPHPD) , which includes articles published by CDC/ATSDR authors since 2011. (cdc.gov)

DNA microarrays measure the relative activity of previously identified target genes. (wikipedia.org)

0.05), and these candidate genes could be used as potential diagnostic biomarkers and therapeutic targets of TAD. (hindawi.com)

Interestingly, while genes involved in immune system function were down-regulated in the brains of mature males, changes in the expression levels of several receptors and channels were observed suggesting that some rewiring is occurring in the brain at sexual maturity. (biomedcentral.com)
Interestingly, most mRNAs involved in lipid metabolism are overexpressed after hatching (FASN, SCD1, ACOX1), whereas genes implicated in carbohydrate metabolism (HK1, GAPDH, GLUT1) and development (HGF, IGF, FGFR2) are predominantly overexpressed from E12 to E20. (biomedcentral.com)
Interestingly, carbohydrate-related genes appear to be highly expressed at the start of kinetics, while most lipid-related genes are overexpress after hatching, revealing greater sensitivity to the food transition that occurs at this stage. (biomedcentral.com)
Interestingly, FLU-exposed biofilms showed no significant changes in gene expression over the course of the experiment. (who.int)

CONCLUSIONS: We found that ICAM1 and other genes may play a role in the development of disc degeneration induced by inflammatory reactions using a bioinformatics analysis of the gene expression profiles of degenerative intervertebral disc cells stimulated with inflammatory factors, suggesting that bioinformatics methods can be used to identify potential target for intervertebral disc degeneration. (minervamedica.it)

In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. (wikipedia.org)
The purpose of this study was to develop a robust, standardized method for comparative expression profiling and molecular characterization of Leishmania donovani clinical isolates. (cambridge.org)
Intracellular amastigote protocols need further refinement, but we describe a first generation tool for high-throughput comparative molecular characterization of patients' isolates, based on the changing expression profiles of promastigotes during in vitro differentiation. (cambridge.org)
This study aims at integrating two gene expression profile datasets and verifying hub genes and pathways involved in TAD as well as exploring potential molecular mechanisms. (hindawi.com)
Here, we have defined a set of genes that help to understand the molecular mechanisms controlling reproduction in eels. (biomedcentral.com)
Since it has been shown that injury-response signal transduction is mediated by gene expression changes, expression profiling is a useful tool to understand the molecular mechanisms of regeneration. (korea.ac.kr)
Innovative UMIs (unique molecular indices) eliminate PCR duplication and amplification bias to deliver the most accurate results (see figure Unbiased and accurate gene quantification (A) Different amounts (20 ng, 5 ng or 1.25 ng) of universal reference RNA were used to determine expression levels of three genes (BNIP1, CTNND2 and DAPK1) using targeted RNAseq. QIAseq digital RNA sequencing method (A; molecular barcode counts) showed accurate quantification of all three genes corresponding to different RNA input, whereas traditional targeted RNAseq (B; read counts) revealed PCR duplication limitation and yielded inaccurate quantification. "> Unbiased and accurate gene quantification ). (qiagen.com)
In fact, UMIs (unique molecular indices) entirely remove PCR bias in RNAseq counting (see figure Unmatched uniformity (>97% of assays are within 20% of median molecular tag counts) A 917-plex gene panel was used to prepare a library from 10 ng of NA12878 reference DNA. All assays were designed to be intra-exon, and are thus single copy on genomic DNA. This allows an estimation of the uniformity of amplicon performance in the library preparation step (e.g., every unique tag equals one captured copy). In terms of raw assay performance, 97.5% of assays are within 20% of mean/median molecular tag counts. For cataloged panels, any assay below 20% is redesigned and replaced. Molecular barcodes entirely remove this variation in RNAseq counting. "> Unmatched uniformity - 97% of assays are within 20% of median molecular tag counts ). (qiagen.com)

Gene expression was related to forced expiratory flow between 25 and 75% of forced expiratory volume (FEF(25-75%) % predicted) revealing a signature gene set of 203 transcripts. (nih.gov)
Transcriptomics has also expanded from sampling protein coding genes to non-coding transcripts such as miRNAs, which have the potential not only to act as more stable biomarkers, but also to regulate translation, and in themselves be used a therapeutic agents against specific targets. (videolectures.net)
With RNA-sequencing now becoming more affordable and with the advantages of identifying novel transcripts and detecting a greater range of expression levels, the future for gene expression profiling includes focusing on transcripts in specific cellular compartments, being an integral part of clinical trials and perhaps even a standard part of diagnostic procedures and therapeutic decisions. (videolectures.net)
Targeted RNA sequencing using UMIs is optimized to deliver highly reliable quantification down to ~100 copies of an RNA target in 25 ng total RNA (see figure Positive results with as little as 0.2 copies of RNA per cell ERCC standards, at 86 to 705,500 copies, spiked into universal reference RNA sample and enriched using 384-plex QIASeq Targeted RNA Panel in three technical replicates. (A) Sensitivity measurement. Under standard conditions (20 ng RNA input, 0.5 million MiSeq reads), ≥~100 copies of ERCC transcripts were reliability detected, which is the equivalent of ~0.2 copies per cell. (B) Precision measurement. At >10 barcodes/gene, CV was less than 5% for all targets, indicating high technical reproducibility. This corresponds to ~100 copies target RNA in the sample. "> Positive results with as little as 0.2 copies of RNA per cell ). (qiagen.com)

Here we present ATAC with select antigen profiling by sequencing (ASAP-seq), a tool to simultaneously profile accessible chromatin and protein levels. (nature.com)
We will explore the distribution of SARS-CoV-2 and Flu reactive cells within gene expression clusters in the Barcode Enabled Antigen Mapping (BEAM) tutorial dataset and identify genes that are upregulated in these antigen reactive cells. (10xgenomics.com)

We developed a computational framework that first arranges existing profiles into a three-dimensional array (or tensor) indexed by drugs, genes, and cell types, and then uses either local (nearest-neighbors) or global (tensor completion) information to predict unmeasured profiles. (nih.gov)

In developing a drug, one may perform gene expression profiling experiments to help assess the drug's toxicity, perhaps by looking for changing levels in the expression of cytochrome P450 genes, which may be a biomarker of drug metabolism. (wikipedia.org)
Fluoride exposure also significantly elevated the protein expressions of cytochrome c and active caspase-3. (greenmedinfo.com)

Furthermore, microarray analysis was also utilized to characterize the effects of fluoride in gene expression profiling on mice sperm. (greenmedinfo.com)
BACHD mice with ubiquitous expression of full-length mutant HTT (mHTT) and wild-type mice with targeted hypothalamic overexpression of either wild-type HTT (wtHTT) or mHTT fragments. (lu.se)
In AAV groups, from the combined limma with GSEA using ClusterProfiler, we found both shared and unique gene sets and pathways for mice with wtHTT overexpression compared to mice with mHTT overexpression. (lu.se)

Finally, we demonstrate that the predicted profiles add value for making downstream associations with drug targets and therapeutic classes. (nih.gov)
On the biological level, we demonstrate that gene expression levels fluctuate during in vitro development of both intracellular amastigotes and promastigotes. (cambridge.org)
Single-cell RNA-sequence analyses demonstrate the homogenous distribution of the gene expression profile in individual cytomegalovirus-specific cytotoxic T-cells within each recipient. (nature.com)

Genes involved in extracellular matrix synthesis/degradation and apoptosis were among the up-regulated genes, whereas genes that participate in antiinflammatory responses were down-regulated. (nih.gov)

BACKGROUND: To investigate the microarray data on the gene expression profiles of intervertebral disc degeneration related to cytokine exposure. (minervamedica.it)

With no hypothesis, there is nothing to disprove, but expression profiling can help to identify a candidate hypothesis for future experiments. (wikipedia.org)
Unsupervised hierarchical clustering was used to identify samples with similar expression profiles. (urotoday.com)
The integrated advanced bioinformatics methods may provide a way to solve the disadvantages and identify the hub genes involved in TAD. (hindawi.com)
The aim of this study was to identify the genes and pathways underlying the growth of the mouse sclera during postnatal development. (molvis.org)
The goal of this study was to identify genes that may be involved in sexual maturation in experimentally matured eels. (biomedcentral.com)
Methods We performed a microarray study to identify and functionally annotate RPE specific gene expression in mouse and human RPE. (vu.nl)
Thus, an experimental setup for the comparative analysis between regenerative and nonregenerative conditions is essential to identify ideal targets for the promotion of regeneration-associated genes and to understand the mechanisms of axon regeneration. (korea.ac.kr)
The study of these genes may help to identify the biochemical pathways that are responsible for pathologic insulin secretion. (elsevierpure.com)
Here we use RNA-seq to identify differences in gene expression in the ovine mammary gland between late pregnancy and lactation . (bvsalud.org)

A popular approach to class discovery involves grouping similar genes or samples together using one of the many existing clustering methods such the traditional k-means or hierarchical clustering, or the more recent MCL. (wikipedia.org)

Approximately 15 slightly different versions (isoforms) of the septin-9 protein may be produced from this gene. (medlineplus.gov)

However, the relation between SEPTIN9 gene mutations and immune function is unclear. (medlineplus.gov)

Gene Set Enrichment Analysis (GSEA) with leading-edge analysis showed that suppressed sterol- and cholesterol metabolism were shared between hypothalamic wtHTT and mHTT overexpression. (lu.se)

Gene expression profiling of in vitro drug perturbations is useful for many biomedical discovery applications including drug repurposing and elucidation of drug mechanisms. (nih.gov)

With the advent of microarrays allowing the expression levels of increasing numbers of genes to be quantified, gene expression profiling, microarray analysis and transcriptomics have become frequently used terms associated with sampling RNA from multiple different tissue types. (videolectures.net)
these often differ significantly from the 'housekeeping' genes commonly used for normalisation in transcriptomics and qPCR studies. (biorxiv.org)

It took me a long time to appreciate just how extensively population heterogeneity and other unmeasured sources of variation introduce unwanted and systematic biases into the statistical analysis of gene-expression studies," Dr. Storey noted. (genengnews.com)
The interaction network analysis indicated that PTGS2, ICAM1, NOV and other genes may play a role in disc degeneration. (minervamedica.it)
The livers of 20 mule duck embryos were collected every 4 days from the 12th day of embryogenesis (E12) until 4 days after hatching (D4), and gene expression analysis was performed. (biomedcentral.com)
Not unexpectedly, our gene expression analysis and comparative functional annotation suggested that, in general, the mouse and human RPE are very similar. (vu.nl)
Cluster analysis of gene expression and analysis of expression ratios was performed. (elsevierpure.com)

The present study contributes to a growing body of work on gene expression signatures in bladder cancer. (urotoday.com)

But even so, gene-expression profiling can be enormously valuable in finding biomarkers, highlighting druggable targets, revealing the metabolic pathways important in a biological process, and identifying subsets of a population that might respond to a certain therapy or be more susceptible to a certain clinical outcome. (genengnews.com)

Sequence based techniques, like RNA-Seq, provide information on the sequences of genes in addition to their expression level. (wikipedia.org)
On the other hand, bulk RNA-sequence analyses reveal that gene expression profiles among patients are different according to the cytomegalovirus reactivation pattern, and are associated with cytokine production or cell division. (nature.com)
Changes in the SEPTIN9 gene may alter the sequence of amino acids in certain septin-9 isoforms in ways that interfere with their function. (medlineplus.gov)

We have manually annotated this dataset in Loupe Browser, using gene markers (also saved with the dataset) to determine cell types. (10xgenomics.com)
The numbers to the right of the clonotype list are the number of cells within that V(D)J clonotype that were also found in the Gene Expression dataset. (10xgenomics.com)

In other words, the scientist is making a specific prediction about levels of expression that could turn out to be false. (wikipedia.org)
Many ZmCAMTA genes displayed high expression levels in the roots. (frontiersin.org)
The expression levels of 50 mRNAs were quantified for these 7 sampling points and classified into 4 major cellular pathways. (biomedcentral.com)
We studied gene expression profiles from insulinomas and determined associations between these changes and preoperative peak serum insulin levels. (elsevierpure.com)
In addition, patients with a distinct and common pattern of gene expression had significantly higher stimulated insulin secretion levels. (elsevierpure.com)
During lactation , high levels of milk fat synthesis were mirrored by enrichment of genes associated with fatty acid biosynthesis , transport and lipogenesis . (bvsalud.org)

The limitations and possibilities associated with expression profiling in intracellular amastigotes and promastigotes were assessed through a series of comparative experiments in which technical and biological parameters were scrutinized. (cambridge.org)
Here, we review the original research that shows the key factors regulating axon regeneration, in particular by using comparative gene expression profiling in diverse systems. (korea.ac.kr)
Lee, J & Cho, Y 2021, ' Comparative gene expression profiling reveals the mechanisms of axon regeneration ', FEBS Journal , vol. 288, no. 16, pp. 4786-4797. (korea.ac.kr)

For this, we used microarrays to compare the gene expression profiles of sexually mature to immature males. (biomedcentral.com)

The set of up-regulated genes includes genes involved in neuroendocrine processes, cell-cell signaling, neurogenesis and development. (biomedcentral.com)

High SEPT9_v1 expression in human breast cancer cells is associated with oncogenic phenotypes. (medlineplus.gov)

We chose to use expression-curves rather than single, specific, time-point measurements to capture this biological variation. (cambridge.org)

Does genomic profiling to assess Type 2 Diabetes risk improve. (cdc.gov)

Multiple biosynthetic pathways such as sterol synthesis were among the top shared processes, where both unique and shared genes constituted leading-edge subsets. (lu.se)

gene expression profiling provides the most global picture possible in a single experiment. (wikipedia.org)
Sometimes, a scientist already has an idea of what is going on, a hypothesis, and he or she performs an expression profiling experiment with the idea of potentially disproving this hypothesis. (wikipedia.org)
One key benefit of running an integrated 5' Gene Expression and V(D)J experiment is the ability to associate phenotypes with clonotypes of interest. (10xgenomics.com)

We will combine our mRNAs expression profile (6 TAD tissues versus 6 non-TAD tissues) and GSE52093 downloaded from the Gene Expression Omnibus (GEO) database. (hindawi.com)
The two mRNAs expression profiles contained 13 TAD aortic tissues and 11 non-TAD tissues. (hindawi.com)

A few SEPTIN9 gene mutations have been identified in individuals with hereditary neuralgic amyotrophy, a disorder characterized by episodes of severe pain and muscle wasting (amyotrophy) in the shoulders and arms. (medlineplus.gov)

Data are available at the Gene Expression Omnibus under accession number GSE156478 . (nature.com)

Most early expression profiling experiments, and many current ones, have this form which is known as class discovery. (wikipedia.org)
The simplest form of class discovery would be to list all the genes that changed by more than a certain amount between two experimental conditions. (wikipedia.org)

Immunohistochemistry confirmed expression of urokinase plasminogen activator (PLAU), urokinase plasminogen activator receptor (PLAUR), and thrombospondin (THBS1) by alveolar macrophages and airway epithelial cells. (nih.gov)
Osteopontin (OPN) expression was validated by immunohistochemistry. (urotoday.com)

his statistical challenge in this study was to correlate gene expression over multiple time points, in multiple patients, with multiple and different clinical presentations. (genengnews.com)
Since most of the genes examined here have orthologs in other vertebrates, the results of this study will contribute to the body of knowledge concerning reproduction in vertebrates as well as to an improved understanding of eel biology. (biomedcentral.com)
To this end, a kinetic study was designed to determine the level of expression of selected genes involved in steatosis-related liver functions throughout embryogenesis. (biomedcentral.com)
Therefore, the aim of the present study was to analyze a wide range of genes involved in liver development, cell stress, lipid and carbohydrate metabolisms throughout embryogenesis in mule ducks to better understand the ontogeny of pathways related to liver fattening. (biomedcentral.com)
Genes and pathways identified by the present study may be important for managing milk production and mammary development, and may inform studies of diseases affecting the mammary gland . (bvsalud.org)

More commonly, expression profiling takes place before enough is known about how genes interact with experimental conditions for a testable hypothesis to exist. (wikipedia.org)

A skewed preference for BV7 genes and sequential "G" amino acids motif is observed in complementarity-determining region-3 of T cell receptor-β. (nature.com)

Alterations in the activity (expression) of the SEPTIN9 gene are associated with certain cancers. (medlineplus.gov)

Structural changes and neuropathology in the hypothalamus have been suggested to contribute to the non-motor manifestations of Huntington's disease (HD), a neurodegenerative disorder caused by an expanded cytosine-adenine-guanine (CAG) repeat in the huntingtin (HTT) gene. (lu.se)
In contrast, the ubiquitous expression of full-length mHTT in the BACHD hypothalamus moderately affects the transcriptomic profile. (lu.se)

The figure above represents the output of a two dimensional cluster, in which similar samples (rows, above) and similar gene probes (columns) were organized so that they would lie close together. (wikipedia.org)
Another way of looking at the relationship between phenotype and clonotype is to filter the clonotype list by a gene expression cluster. (10xgenomics.com)

For instance, skin cells, liver cells and nerve cells turn on (express) somewhat different genes and that is in large part what makes them different. (wikipedia.org)

We will investigate the clonality and diversity of lymphocytes within a non-small cell lung carcinoma sample, look at the distribution of clonotypes within two T cell phenotypes, and explore a well-conserved clonotype motif in the gene expression data. (10xgenomics.com)
Reporter gene assay of pck2 400 bp fragment containing GCGGAGGC motif (A) and myogenin fragment containing CGACCCGT motif (B). There is a change in the reporter activity upon mutagenesis in pck2 constuct and there is no change in myogenin construct. (biomedcentral.com)

Researchers around the globe are harnessing gene-expression profiling studies in a variety of clinical settings to prevent and treat a number of diseases. (genengnews.com)
Gene expression profiling is increasingly used in the field of infectious diseases for characterization of host, pathogen and the nature of their interaction. (cambridge.org)

Gene expression profiling may become an important diagnostic test. (wikipedia.org)

Anatomy21

Organisms15

Diseases21

Chemicals and Drugs48

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes96

Disciplines and Occupations6

Technology, Industry, Agriculture2

Information Science9

Health Care9

Differentially18

Regulation5

Transcriptome2

Transcriptional4

MRNA9

Proteins5

Quantitative4

Pathway7

Patterns5

Specific genes4

Genome6

Significantly downregulated1

Genetic3

Genomics2

Target genes1

Candidate genes1

Interestingly4

Conclusions1

Molecular8

Transcripts4

Antigen2

Computational1

Cytochrome2

Mice3

Demonstrate3

Apoptosis1

Microarray data1

Identify9

Existing cluster1

Protein1

Immune1

Enrichment Analysis1

Vitro1

Transcriptomics2

Analysis5

Signatures1

Biomarkers1

Sequence3

Dataset2

Levels6

Comparative3

Microarrays1

Neuroendocrine1

Breast cancer1

Biological1

Assess1

Synthesis1

Experiment3

MRNAs2

Mutations1

Omnibus1

Discovery2

Immunohistochemistry2

Study5

Commonly1

Amino acids1

Cancers1

Hypothalamus2

Cluster2

Nerve1

Motif2

Diseases2

Diagnostic1