• Te identifier uses polymerase chain reaction for higher fidelity identification of biological samples, while the lateral flow assays can screen for bioagents of concern. (army.mil)
  • We provide half or full day workshops showcasing polymerase chain reaction (PCR), enzyme assays, gel electrophoresis and some experimental techniques used in research laboratories. (reading.ac.uk)
  • Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. (illinois.edu)
  • Here, we investigate the association of leukocyte telomere length with schizophrenia with the quantitative polymerase chain reaction method in independent Japanese cohorts consisting of 1,241 patients with schizophrenia and 1,042 controls, which we believe is the largest ever as an independent sample set of telomere studies in schizophrenia. (databasefootball.com)
  • Using polymerase chain reaction Mycoplasma hominis and Ureaplasma urealyticum were detected in cervical secretions of 19.2% and 13.7% of infertile women, and the presence of mycoplasma was significantly correlated with the presence of AZP-Ab and ASA. (who.int)
  • An addition to the existing requirements was made by introducing a new molecular technique, called mutant analysis by polymerase chain reaction and restriction enzyme cleavage (MAPREC), for the quality control of the vaccine. (who.int)
  • The suspected pertussis cases, reported by health workers from the Ononge health centre area, were investigated and confirmed for the presence of Bordetella pertussis DNA using the polymerase chain reaction (PCR) method. (who.int)
  • Polymerase chain reaction: locates and makes copies of parts of the DNA contained in the castor bean plant. (cdc.gov)
  • Major candidates were then re-evaluated by absolute quantitative polymerase chain reaction analysis in an independent cohort of patients with PTC (n = 44) or benign nodules and 20 healthy controls (HCs). (medscape.com)
  • RNA sequencing versus real-time polymerase chain reaction versus microarrays, as well as miRNA profiling versus selected miRNA panels) and other methodological differences ( e.g. , characteristics and size of patient and control groups, sampling time, or use of plasma or serum). (medscape.com)
  • Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. (thermofisher.com)
  • Such cytokine secretion is involved in the induction of immune reactions to foreign proteins at the injection site. (ddw-online.com)
  • FA is an adverse reaction that results from an inappropriate and excessive immune response against dietary proteins. (frontiersin.org)
  • This reproducible immune reaction results from an impaired induction of oral tolerance, i.e., a suppressive immune process at local and systemic levels that physiologically allows harmless dietary proteins to be tolerated by the immune system, thus avoiding chronic intestinal inflammation due to their regular consumption. (frontiersin.org)
  • Dubbed the Biology/Disease-Driven Human Proteome Project, or B/D-HPP, this new arm aims to organize characterization of the proteome based on proteins' roles in disease and biological functions. (genomeweb.com)
  • It stems from the notion, Aebersold said in a presentation to the congress, that, while currently, "most biological research is focused on [individual] biological molecules … that is changing very quickly, and the focus is clearly shifting to networks, especially in the field of proteomics where proteins clearly act in coordination with other proteins to convert the phenotype. (genomeweb.com)
  • Here, we describe the generation and verification of a compendium of highly specific assays that enable quantification of 99.7% of the 20,277 annotated human proteins by the widely accessible, sensitive, and robust targeted mass spectrometric method selected reaction monitoring, SRM. (nih.gov)
  • Such inactivated reference materials were used as positive controls in assays to detect suspected B. anthracis in samples because live agent cannot be shipped for use in field settings, in improvement of currently deployed detection methods or development of new methods, or for quality assurance and training activities. (cdc.gov)
  • Early detection of a biological threat is critical not only for identifying the threat organism but also for implementing appropriate countermeasures to save and protect the victims and prevent further infection and for decontaminating and reclamating the affected environment and infrastructures. (cdc.gov)
  • The bedrock of successful biodetection platforms and sensors is use of well-characterized molecular assays, immunoassays, or other types of detection assays. (cdc.gov)
  • Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay. (gentaur-online.com)
  • Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl - in 96-well plate assay. (gentaur-online.com)
  • Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H 2 O 2 in 96-well plate assay. (gentaur-online.com)
  • Linear detection range 0.12 µM (0.78 µg/dL) to 10 µM (65 µg/dL) zinc in 96-well assay format. (immpact-international.org)
  • Perform your enzyme reaction, add the detection reagents, and measure. (bellbrooklabs.com)
  • The Detection Mix was added with the substrate to start the reaction and incubated at 30°C. Readings were taken with a CLARIOstar plate reader. (bellbrooklabs.com)
  • There are no specific clinically validated assays for detection of ricin that can be performed by the hospital/healthcare facility clinical laboratory. (cdc.gov)
  • Description: A competitive ELISA for quantitative measurement of Human Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (gentaur-online.com)
  • Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. (gentaur-online.com)
  • ExoTEST is a patented double sandwich ELISA assay for quantitative and qualitative analysis of exosomes. (immpact-international.org)
  • ExoTEST consists of ELISA plates pre-coated with patented panexosome antibodies that allow specific capture of exosomes from a variety of biological samples, including cell culture supernatants and human biological fluids. (immpact-international.org)
  • Usually, human primary immune cells are employed for in vitro biological assays for immunogenicity prediction, and though they are generally obtained from healthy donors, samples from specific subpopulations of patients can be of interest in the study of disease-specific immunogenic reactions. (ddw-online.com)
  • The antigen priming of CD4+ T cells by mature MoDCs leads to the proliferation of CD4+ T cells in in-vitro PBMC assays. (ddw-online.com)
  • MAPPS) assays use purified HLA-II peptides of the protein of interest to complement in silico identification and evaluation of the binding affinity of HLA-II peptide epitopes. (ddw-online.com)
  • We report data on 166,174 proteotypic peptides providing multiple, independent assays to quantify any human protein and numerous spliced variants, non-synonymous mutations, and post-translational modifications. (nih.gov)
  • Enhanced chemiluminescent assay for antioxidant capacity in biological fluids. (wikipedia.org)
  • The present invention provides an analytical test device for conducting assays of biological fluids. (patsnap.com)
  • In particular ExoTEST is a successful platform for exosomes quantification and characterization from small amount of human biological fluids or cell media and it may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. (immpact-international.org)
  • The tissue-specific expression patterns of these RNA species and their high stability in biological fluids are well established. (medscape.com)
  • The review is summarized and systematized the published data on the multicomponent reactions of ethyl trifluoroacetoacetate, carbonyl compounds (aldehydes and ketones), and nucleophilic reagents. (researchgate.net)
  • The silver enhancement reagents may be integrated into the microfluidic assay platform to be released upon sample addition. (hindawi.com)
  • Human IgG antibody Laboratories manufactures the quantichrom ™ magnesium assay kit for approx 250 reactions manufacturer bioassay systems biotrend catalogue number dimg 250 st reagents distributed by Genprice. (gentaur-online.com)
  • Run your enzyme reaction, add Transcreener reagents, and read your plates. (bellbrooklabs.com)
  • Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat. (cytoskeleton.com)
  • During chemical experiment, test tube is most commonly seen experiment equipment, test tube, the conventional instrument of chemical laboratory Device, serves as the reaction vessel of a small amount of reagent, (should be preheated before heating, not so test tube is easily quick-fried in normal temperature or heating Split. (google.com)
  • The Quantichrom ™ Magnesium Assay Kit For Approx 250 Reactions Manufacturer Bioassay Systems Biotrend Catalogue Number Dimg 250 St reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (gentaur-online.com)
  • BIOLOGICAL DATA AND TOXICOLOGICAL EVALUATION Biochemical aspects Some knowledge has been obtained of the changes which the compound undergoes in plants. (inchem.org)
  • A microfluidic system for performing chemical reactions or biochemical, biological, or chemical assays utilizing a microfabricated device or "chip. (patsnap.com)
  • Can the 'standard deviation' or the assay itself be calculated from these data, as if they were direct, repeated measurements? (physicsforums.com)
  • The low relative standard deviation values of inter-day and intra-day assays highlighted the good reproducibility of the proposed method for assay of nevirapine. (bgu.ac.il)
  • To test the hemagglutination reaction, two types of ConA solutions were compared, a purchased control ConA solution in buffer as the positive control, and a purified solution of ConA in buffer previously purified in lab. (studyscroll.com)
  • Each solution was at a 2mg/ml concentration of ConA in ConA buffer, which is necessary for ConA's biological activity. (studyscroll.com)
  • 1. Add 5 mL of Assay Buffer (Component C) into Enzyme Mix bottle (Component B), and mix well. (aatbio.com)
  • Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)
  • The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. (anaspec.com)
  • The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. (patsnap.com)
  • This human SRMAtlas provides definitive coordinates that conclusively identify the respective peptide in biological samples. (nih.gov)
  • The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds. (anaspec.com)
  • Samples: Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc). (gentaur-online.com)
  • Can be readily automated as a high-throughput assay for thousands of samples per day. (gentaur-online.com)
  • Low interference in biological samples. (gentaur-online.com)
  • Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. (gentaur-online.com)
  • Samples: Biological, food and environment. (gentaur-online.com)
  • It provides a robust, simple and convenient assay for measuring ATP levels in biological samples. (aatbio.com)
  • Add 50 µL of ATP working solution to each well of ATP standard, blank control, and test samples to make the total ATP assay volume of 100 µL/well. (aatbio.com)
  • Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples. (bellbrooklabs.com)
  • The potential uses of tests for either ricin or ricinine in human biological samples would be primarily for purposes of confirming exposure or assessing the prevalence of exposure rather than diagnostic use. (cdc.gov)
  • Trends toward more conservative management of papillary thyroid cancer (PTC) diminish the primacy of serum thyroglobulin (Tg) assays as a posttreatment surveillance tool. (medscape.com)
  • miR-146a-5p and miR-221-3p hold remarkable promise as serum biomarkers for post-treatment monitoring of PTC patients, especially when Tg assay results are uninformative. (medscape.com)
  • Furthermore, since reactions occur in real time with CSTRs, their residence times are much shorter compared to batch processing methods. (labbulletin.com)
  • This inconsistency may be partially due to the relatively small sample sizes in each study and heterogeneity caused by various uncontrolled confounders (e.g., duration of illness or hospitalization, lifetime antipsychotic dose, and telomere length assay methods). (databasefootball.com)
  • Methods for carrying out the assays with the disclosed analytical test device are also provided. (patsnap.com)
  • The primary reasons for the preferment of the LLNA are the animal welfare benefits it provides compared with traditional guinea-pig methods (refinement and reduction of animal usage) and the general performance characteristics of the assay with regard to overall reliability, accuracy, and interpretation. (cdc.gov)
  • Nano-TiO2 also formed small agglomerates in medium containing as little as 1% protein and caused cellular interaction as measured by side scatter by flow cytometry and DNA damage (comet assay) in HepG2 cells. (cdc.gov)
  • GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. (thermofisher.com)
  • b) Schematic drawing of a bioanalytical REA assay before (left) and after (right) binding of AuNP-analyte complexes to the capture protein layer. (hindawi.com)
  • This dissertation describes the fabrication, characterization, development, and application of silicon photonic microring resonator technology to multiplexed protein measurements in a variety of biological systems. (illinois.edu)
  • The objective of this study was to examine the influence of component of solid lipid nanoparticles on the viability of breast cancer cells using the MTT assay. (scialert.net)
  • These kits require luminescence plate readers, are frequently used for cell viability or cytotoxicity assays. (aatbio.com)
  • This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. (anaspec.com)
  • The Transcreener Rac1 Assay determines Rac1 enzyme activity by directly measuring the GDP formed by the enzyme using the Transcreener GDP Assay. (bellbrooklabs.com)
  • These cells allow different types of assays to be performed, covering the T cell-mediated immune response from antigen uptake to T cell proliferation. (ddw-online.com)
  • Particle-enhanced assays are based on the reaction between a soluble analyte and the corresponding antigen or antibody bound to polystyrene particles. (cdc.gov)
  • In particular, the biological assay of potency recommended in 1991 had been shown not to correlate with the efficacy of the vaccine in infants, nor to provide a sensitive indicator of vaccine quality. (who.int)
  • In one run of the assay M molecules are tested, generating 2 M N data points. (physicsforums.com)
  • The assay is often repeated multiple times on certain molecules. (physicsforums.com)
  • Biomolecular simulation is increasingly central to understanding and designing biological molecules and their interactions. (manchester.ac.uk)
  • We constructed a genetically inactivated or attenuated strain containing relevant molecular assay targets and tested to compare assay performance using this strain to the historical data obtained using irradiation-inactivated virulent spores. (cdc.gov)
  • The assay can detect ~3 µM of ATP in a 100 µL reaction volume with minimal interference from ADP and AMP. (aatbio.com)
  • Rac1 enzyme is not included in the inhibitor screening assay kit. (bellbrooklabs.com)
  • By sequence similarity analysis and functional assays from the species Streptomyces lividans TK23, S. coelicolor A3(2) and S. glaucescens GLA.O, first hints for a widespread lack of MalP and presence of AmlE in the class Actinobacteria were given. (frontiersin.org)
  • ECL reactions are used in a number of clinical and analytical applications and are based upon the oxidation of luminol in the presence of the enzyme horseradish peroxidase (HRP), an oxygen source, and an enhancer such as 4-iodophenol. (wikipedia.org)
  • A structural-activity relationship for the estimation of rate constants for the gas-phase reactions of OH radicals with organic compounds. (cdc.gov)
  • This structural information is then used to estimate the modifications of the reaction rate constants and the corresponding free-energy profiles of CA II catalysis. (iucr.org)
  • cytokine quantitation is thus important in fundamental biological studies and diagnostics, and complex and overlapping cytokine roles make multiplexed measurements especially vital. (illinois.edu)
  • Luminescence test measurements are conducted using an assay module having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence. (patsnap.com)
  • To further display the wide array of sensing capabilities that could be used to carry out the mission, handheld lateral-flow immuno- assays were available in the DR SKO for presumptive screening of biological hazards, along with the Joint Handheld Biological Identifier. (army.mil)
  • Any assay development effort requires testing, evaluation, and validation of the assays with live or inactivated spiking materials in appropriate matrices relevant to the environments in which the assays are intended to be used (e.g., aerosol collection filters, soils, or clinical matrices). (cdc.gov)
  • Questions of biological and clinical relevance predominated at the Human Proteome Organization's 10th annual meeting this week in Geneva, as much of the discussion focused on efforts to move the field of proteomics in more applied directions. (genomeweb.com)
  • these biological variables will be analyzed for independent clinical significance compared to MYCN amplification, International Neuroblastoma Staging System (INSS) stage, age, ploidy, and histologic variables in predicting either response to treatment or outcome. (uchicagomedicine.org)
  • Other articles in the CBRNE section (see CBRNE - Biological Warfare Agents and CBRNE - Evaluation of a Biological Warfare Victim) discuss the specific clinical management issues involved with treatment of patients exposed to potential bioterrorism pathogens and toxins. (medscape.com)
  • The use of international reference materials for designating the activity of biological substances used in prophylaxis or therapy, or for ensuring the reliability of diagnostic procedures, allows comparability of data globally. (who.int)
  • For the past few years, research on the pathogenic mechanism of C. cassiicola has mainly focused on biological characteristics, pathogenicity differentiation, cloning of virulence-associated genes, etc. (biomedcentral.com)
  • The goal of the MLP is to screen compounds in the Small Molecule Repository in target-based and phenotypic assays to identify and subsequently optimize small molecule s as research probes. (nih.gov)
  • In this study, using the Hain GenoType MTBDRsl assays (versions 1 and 2), we found that some nonsynonymous and synonymous mutations in gyrA in Mycobacterium tuberculosis result in systematic false-resistance results to fluoroquinolones by preventing the binding of wild-type probes. (ox.ac.uk)
  • The biological activity of gelsolin is determined in an F-actin severing assay. (cytoskeleton.com)
  • Gelsolin biological activity determination. (cytoskeleton.com)
  • Three series of bicyclic, semirigid congeners of beta-phenethylamine have been prepared for evaluation of the effect of ring size (and of concomitant conformational variation) on biological activity in a variety of assays for adrenergic and dopaminergic actions. (erowid.org)
  • Simulations can now be used as computational assays of biological activity, for example, in predictions of drug resistance. (manchester.ac.uk)
  • The SensoLyte® Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. (anaspec.com)
  • Significant insight into their catalytic mechanisms has been obtained by relating site-directed mutagenesis studies to kinetic activity assays. (iucr.org)
  • The purpose of this lab was to test the biological activity of ConA by performing a hemagglutination assay. (studyscroll.com)
  • The results did not support my hypothesis for the biological activity of ConA. (studyscroll.com)
  • In conclusion, the results did not clearly explain the biological activity of ConA with the hemagglutination assay. (studyscroll.com)
  • By detecting GDP output, the assay provides a universal method to assess the activity of any GDP-producing enzyme in real time. (bellbrooklabs.com)
  • The present approach shows the significant outcomes such as yield up to 94%, catalyst‐free reaction, easy workup procedure and shorter period of reaction time. (researchgate.net)
  • Procedure: Assay takes 30 min. (gentaur-online.com)
  • The increasing complexity and sophistication of biological/biotechnological substances used in human medicine, and the rapid growth in their volume, present a considerable challenge for regulatory authorities, especially in the developing world. (who.int)
  • Recommendations published by WHO are intended to provide guidance for national regulatory authorities and for manufacturers of biological products. (who.int)
  • For many regulatory authorities, the local lymph node assay (LLNA) is the preferred assay for the predictive identification of skin-sensitizing chemicals. (cdc.gov)
  • These assays are performed on a Behring Nephelometer for quantitative CRP determination. (cdc.gov)
  • A close relationship was found between this property and infection with the type 2 strains and it is hoped that this observation may be used as a simple and reliable biological marker for the differentiation of HVH type 1 and type 2. (microbiologyresearch.org)
  • Biological markers for differentiation of herpes-virus strains of oral and genital origin. (microbiologyresearch.org)
  • The SLNs based on palm oil were prepared using the high pressure homogenization method and were characterized by the particle size and polydispersity index (particle sizer), zeta potential (zetasizer), ultrastructure [transmission electron microscopy (TEM)] and MTT assay and natural red assay. (scialert.net)
  • Transcreener Rac1 Assay technology uses a simple but highly effective method that consists of an antibody selective to GDP and a far-red fluorescent tracer. (bellbrooklabs.com)
  • PhosphoWorks™ Colorimetric ATP Assay Kit is based on a serial ATP-induced enzyme coupled reactions to produce hydrogen peroxide, which is spectrophotometrically quantified with our Amplite® Red Substrate at OD 570 nm. (aatbio.com)
  • Transcreener GDP Assay directly measures GDP produced by Rac1 (Ras-related C3 botulinum toxin substrate 1). (bellbrooklabs.com)
  • These coagulant reactions are regulated by a number of stimulatory and inhibitory mechanisms. (medscape.com)
  • PBMC, DC:T) assay makes use of APC surface expression of specific antigens, eg. (ddw-online.com)
  • A specific assay for factor VII, using known factor VII-deficient plasma, is required to confirm the diagnosis. (medscape.com)
  • 1994. Threshold limit values for chemical substances and physical agents and biological exposure indices (1994- 1995). (cdc.gov)
  • Hi everyone, I have a question about the error on certain biological results. (physicsforums.com)
  • When the absolute amounts of standard used in the assay are known, the results of the assay can be used to estimate the amount - in absolute units - of biologically active material contained in the unknown preparation. (pharmacologycorner.com)
  • The results did show that a change in the concentration of ConA would alter the strength of the reaction. (studyscroll.com)
  • Some Synonymous and Nonsynonymous gyrA Mutations in Mycobacterium tuberculosis Lead to Systematic False-Positive Fluoroquinolone Resistance Results with the Hain GenoType MTBDRsl Assays. (ox.ac.uk)
  • PLOS Biology provides an Open Access platform to showcase your best research and commentary across all areas of biological science. (plos.org)
  • Microsomal GST-catalyzed reaction of the compound resulted in formation of diastereomeric GST-conjugates. (syncsci.com)
  • The assay provides a powerful tool to screen compound libraries for Rac1 modulators to help find new therapies for disease. (bellbrooklabs.com)
  • GDP produced in the reaction competes with the tracer changing the fluorescent properties and providing fluorescent readout. (bellbrooklabs.com)
  • Cellular interactions and biological responses to titanium dioxide nanoparticle s in HepG2 and BEAS-2B cells: role of cell culture media. (cdc.gov)
  • Cell Biology 3822 Analysis of purified ConA via Hemagglutinatino Assay Lab 7: Powerpoint. (studyscroll.com)
  • In this study, we describe the effect of a single amino-acid variation on a prototypical enzyme, human carbonic anhydrase II (CA II), by correlating its high-resolution reaction-intermediate structures with the measured kinetic parameters. (iucr.org)
  • 1994. Biological monitoring of exposure to organophosphorus insecticides by assay of urinary alkylphosphates: influence of protective measures during manual operations with treated plants. (cdc.gov)
  • Reliability of the Eclox Enhanced Chemiluminescence Assay for Rapid Field Testing of Drinking Water, Thesis, Texas A&M University System Health Science Center Whitehead, T.P., Thorpe, G.H.G., Maxwell, S.R.J., 1992. (wikipedia.org)
  • Silver enhancement of the bound nanoparticles may be used to increase the sensitivity of the assay. (hindawi.com)
  • An effective and constant real-time surveillance capability is crucial for protecting the public from biological threats. (cdc.gov)
  • the precision of the estimate should always be determined, using the data of the assay. (pharmacologycorner.com)
  • The assay demonstrates linearity when raw data is converted to GDP using a standard curve. (bellbrooklabs.com)
  • AAT Bioquest offers a variety of bioluminescence assay kits to determine nanomolar (nM) range of ATP with recombinant firefly luciferase (Cat# 21610 & 21609). (aatbio.com)
  • 1990. Subcommittee report on biological indicators of organ damage. (cdc.gov)
  • The biological indicators in bioassay are the reactions of living organisms or tissues. (pharmacologycorner.com)
  • The recent advances in the study of the Hantzsch and Biginelli reactions involving polyfluoroalkyl-3-oxo esters are analyzed. (researchgate.net)
  • The assay conditions below are chosen to generate the best assay window and ability to study the enzyme. (bellbrooklabs.com)
  • The Enhanced Chemiluminescence (ECL) Water Quality Test: Modes of Action and Mechanistic Studies of Substances acting on the Enhanced Chemiluminescence Reaction. (wikipedia.org)