• transgenic
  • Extracellular matrix (ECM) molecules and their integrin cell surface receptors are important regulators of these aspects of cell behaviour, and genetic studies using naturally occurring mutants or transgenic mice have revealed essential roles in development for some of the ECM glycoproteins and integrins expressed in the CNS. (biologists.org)
  • To investigate its functional role, we generated AICD transgenic mice, and found that AICD causes significant biologic changes in vivo. (rupress.org)
  • AICD transgenic mice show activation of glycogen synthase kinase-3β (GSK-3β) and phosphorylation of CRMP2 protein, a GSK-3β substrate that plays a crucial role in Semaphorin3a-mediated axonal guidance. (rupress.org)
  • To examine the in vivo role of AICD, we generated transgenic mice that express AICD and Fe65 in the forebrain and hippocampal regions of the postnatal brain. (rupress.org)
  • We report that the transgenic mice show two- to threefold higher levels of AICD than control mice, and display robust activation of glycogen synthase kinase-3β (GSK-3β) and increased phosphorylation of a downstream substrate, CRMP2, a key component of the axonal guidance signaling pathway. (rupress.org)
  • To examine the in vivo effects of AICD, we generated double transgenic mice expressing AICD and Fe65. (rupress.org)
  • The specificity of AICD effects can be determined by comparing the AICD+Fe65 double transgenic mice with Fe65 single transgenic animals. (rupress.org)
  • The Transgenic Mouse / ES Cell Shared Resource assists investigators in generating, maintaining and storing germline-altered mice. (vanderbilt.edu)
  • This resource, which has been in existence for over 23 years, has generated over 2700 transgenic founder mice from over 750 different DNA constructs and 5000 chimeric mice from over 800 different mouse ES cell clones. (vanderbilt.edu)
  • The Vanderbilt Transgenic Mouse/ES Cell Shared Resource (TMESCSR) provides services, consultation and collaborations to enable the generation, storage and regeneration of genetically altered mice at Vanderbilt. (vanderbilt.edu)
  • We have many years of experience in generating novel transgenic mouse models and are happy to discuss your project with you. (vanderbilt.edu)
  • The Transgenic Mouse/ES Cell Shared Resource is supported by NIH grants DK020593 and CA68485. (vanderbilt.edu)
  • vivo
  • Despite multiple effects of tenascin-C on cell behaviour in culture, no structural abnormalities of the CNS and other organs have been found in adult tenascin-C-null mice, raising the question of whether this glycoprotein has a significant role in vivo. (biologists.org)
  • A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo. (vanderbilt.edu)
  • show
  • Knockout mice show increased rates of oligodendrocyte precursor migration along the optic nerve and reduced rates of oligodendrocyte precursor proliferation in different regions of the CNS. (biologists.org)
  • cell
  • We are sorry, but due to low utilization we can no longer provide mouse ES cell-based services on a routine, fee-for-service basis (please see notification letter ). (vanderbilt.edu)