Device high throughput flow cytometry. Medical search. Frequent questions

Conventional flow cytometer has the advantage of high throughput: detect single cell properties at rates from hundreds to 100,000 cells per second. (e-consystems.com)
Also, while a conventional flow cytometer helps in multi-parametric analysis, it lacks spatial resolution. (e-consystems.com)
An ultra-high throughput imaging cytometer that uses stroboscopic lighting and inertial focusing allows sheathless manipulation of cells with no motion blur. (e-consystems.com)
To successfully carry out a large proportion of the applications mentioned above, you will need a high performance flow cytometer such as the ZE5 Cell Analyzer. (bio-rad.com)
Critical for helping scientists accelerate scientific and drug discoveries in both academia and industry, the ZE5 Cell Analyzer is a high performance, automation-ready flow cytometer with the ideal combination of high-throughput screening and high parameter cell analysis capabilities. (bio-rad.com)
The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)
Moreover, inclusion of a high throughput imaging cytometer allows for many of these same comparisons to be made without the need for trypsinization and extensive cell processing. (utsouthwestern.edu)
Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)
The flow cytometer is equipped with multiple lasers for analysis of multi-fluorescent labelled cell populations and acoustical focusing for improved cell separations. (utsouthwestern.edu)
The next-generation mass cytometer has brought in a revolution in the industry with its high-throughput power and full-spectrum acquisition to allow for the simultaneous analysis of 40+ parameters at a single-cell resolution. (weeklyreviewer.com)
Indianapolis (December 14, 2015) - A leading European gene therapy research team has won a $200,000 CytoFLEX Flow Cytometer from Beckman Coulter Life Sciences. (beckman.com)
The CytoFLEX Flow Cytometer measures up to 15 parameters with 13 colors for fluorescence detection, enabling it to detect and discriminate minute subpopulations. (beckman.com)

His group has pioneered the use of microfluidic systems for small molecule chemistry and nanomaterial synthesis, and in recent years has been at the forefront of developments in droplet-based microfluidics for ultra-high-throughput biological experimentation. (wikipedia.org)

A key theme of deMello's research has been the development of ultra-high sensitivity detection methods for use in microfluidic and nanofluidic environments. (wikipedia.org)
Flow cytometry systems that use a charge coupled device (CCD)-camera have greatly enhanced sensitivity and increase the limit of detection. (selectbiosciences.com)
Impedance flow cytometry (IFC) has been widely adopted for high-throughput cell detection and manipulation, sorting and counting. (polito.it)
Learn the principles of exosome detection using flow cytometry, and access a range of useful resources on small particle analysis, including a handy protocol. (bio-rad.com)
Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. (mdpi.com)
In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution-detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. (mdpi.com)
The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. (mdpi.com)
This technique also delivers an inherently high signal-to-noise ratio because the laser and detection wavelengths are widely separated. (photonics.com)
bacteria culture is the gold standard in TB detection for its highest sensitivity, but requires weeks to obtain results. (cdc.gov)
MTB), and thereby enabling high detection specificity and versatility. (cdc.gov)

Schramm T, Lempp M, Beuter D, Sierra SG, Glatter T, Link H (2020), High-throughput enrichment of temperature-sensitive argininosuccinate synthetase for two-stage citrulline production in E. coli. (uni-marburg.de)

SHANGHAI , Oct. 23, 2021 /PRNewswire/ - Polaris Biology, a leading innovator in single-cell technologies, announced today the largest product launch in company history with the introduction of the Starion™ Mass Cytometry System. (weeklyreviewer.com)

Drug screening efforts have traditionally relied primarily on time-consuming or computationally demanding techniques like ELISA-type assays and high-content imaging cytometry. (labmanager.com)

These advances enable high-throughput phenotypic screening and structure-activity relationship screening (SAR) in areas like drug discovery, therapeutics development, and biomarker discovery. (labmanager.com)
Flow cytometry has only recently become a viable method for high-throughput screening. (labmanager.com)
Incorporating imaging capabilities into conventional flow cytometry is highly recommended since cellular morphology analysis is crucial for many biological studies and clinical diagnoses, including cancer screening. (e-consystems.com)
Therapeutics development, antibody screening, and biomarker discovery demand maximal productivity to reduce time-to-results, which can greatly benefit from high-throughput, high parametric flow analysis interfaced with robotic automation. (bio-rad.com)
In the pharmaceutical industry, high-throughput screening is key to accelerating and reducing the cost of drug discovery. (photonics.com)
Using a combination of rational design and high-throughput screening we optimize the switching circuits of engineered cells and connect their activity to the release of immune checkpoint inhibitors. (nature.com)
The MLP anticipates funding 6-10 centers, (i.e. 2-4 comprehensive, high throughput centers, 2-3 high value target, lower throughput specialized screening centers, and 2-3 specialized chemistry centers). (nih.gov)

Highlights in this large body of work include the development of fluorescence lifetime imaging methods, the application of high sensitivity vibrational spectroscopies to small volume analysis and the development integrated optics for point-of-care diagnostic technologies. (wikipedia.org)
IFC combines the single-cell identification and high throughput of traditional flow cytometry with the cell image collection capabilities of fluorescence microscopy. (e-consystems.com)
Optical components from Edmund Optics ® are used in countless Life Science-related applications and medical devices such as qPCR instruments, fluorescence microscopes (confocal, multi-photon, super-resolution), flow cytometers (cell sorting), ophthalmoscopes, optical coherence tomography (OCT), and many more. (edmundoptics.eu)
Fluorescence activated cell sorting (FACS) is a specific branch of flow cytometry that actively sorts a heterogeneous collection of cells into various containers a single cell at a time. (edmundoptics.eu)
Lab-On-a-Chip (LoC) devices are advantageous as they can offer a wide range of analysis tools from cells to molecules (for instance, based on mechanical, electrical, and fluorescence properties). (fluigent.com)
Flow cytometry is a laser-based technology that allows the simultaneous measurement of fluorescence in single cells at different wavelengths reporting the cellular concentration of several fluorescently-tagged molecules, and of other physical or biological parameters such as cell size or granularity in a statistically robust fashion. (uni-marburg.de)
This is a massive advancement from the traditional fluorescence-based flow cytometry, making it ideal for the use in healthcare, academic, and scientific research settings. (weeklyreviewer.com)

Here we review the key recent innovations that ensure reliable, high-throughput, automated workflows. (labmanager.com)
Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation. (curiox.com)

High magnification microscopy can analyze EVs, but has very low throughput. (selectbiosciences.com)
They help combine the high throughput of traditional flow cytometry methods with the spatial resolution of optical microscopy. (e-consystems.com)
In clinical diagnostics and cell biology, the imaging strategies (such as the speed of the laser scanning technologies used in laser confocal microscopy), the frame rate of the image sensors, the complexity of the image processing, the computing power and optical blur brought on by cells moving at fast linear speeds are what primarily limit the imaging throughput. (e-consystems.com)
Photobleaching and low penetration depth, caused by the high scattering of the visible light, limit the utility of confocal microscopy in certain applications, particularly with living cells and/or thicker tissue samples. (photonics.com)
Analysis of urine particle which is microscopy-based has progressed steadily over the years, enabling clinical laboratories' throughput. (medgadget.com)
As an alternative to automated microscopy, urinary flow cytometric data analysis has helped in the differentiation of distinct urinary microorganisms. (medgadget.com)
Instruments based on flow cytometry and microscopy generate effective and clinically useful results, whereas the reading of automated test strip provides further value. (medgadget.com)

In combination with microfluidics, quantitative phase imaging is used to monitor high throughput flowing cells. (duke.edu)
fabricated an electrical impedance flow cytometry device based on microfluidics with a constrictied microchannel to simultaneously characterize the mechanical and electrical properties of single plant cells 1 . (fluigent.com)

Bio-Rad's high-throughput ZE 5 Cell Analyzer solved this with an integrated wash station that travels with the probe, cleaning it thoroughly between each sample. (labmanager.com)
Instrument blocking has formed another major concern, according to Richard, who reports further innovation in sample pumps along with increased pressure and speed in flow cells within the ZE 5 Cell Analyzer to mitigate this problem. (labmanager.com)

Flow cytometry is currently the only method building in rigor and reproducibility for single EV measurements, but older PMT-based flow cytometers have limitations in detecting EVS due to low sensitivity, cutting out the smallest of particles. (selectbiosciences.com)
These devices find it tough to distinguish particles or cells close in size as the spatial resolution is determined by the size of the laser beam and the optics used to detect scattered light. (e-consystems.com)
Hence, the ability of traditional flow cytometers to discriminate between particles of similar size becomes challenging. (e-consystems.com)
Flow Cytometry is an analytical technique used in a variety of life science applications for counting, inspecting or sorting particles in solution such as single cells. (edmundoptics.eu)
Qualitative and quantitative data is collected as the particles flow through a laser beam and forward and side scattered light is collected. (edmundoptics.eu)
Flow Cytometry is an extremely effective method of recognizing and assessing characteristics of cells or particles. (bio-rad.com)
The first particle separator using the flow cytometry technology was employed in 1965 at Los Alamos National Laboratory ( 1 ) for sorting particles with different volumes to meet the needs of Mack Fulwyler. (frontiersin.org)

The performance of the microfluidic device is tested in a fluidic system with microbeads of different sizes (4-6-8 μm). (polito.it)
Based on this phenomenon, we developed a portable capillary-driven microfluidic device that separates blood microsamples collected from finger-pricks and delivers 2 µL of metered serum for bench-top analysis. (researchgate.net)
NanoFabTx™ reagent and microfluidic device kits provide a ready-to-use platform to screen multiple formulations, and ultimately synthesize mRNA or drug-encapsulated liposomes for preclinical assessments. (sigmaaldrich.com)

We demonstrate a label free and high-throughput microbubble-based acoustic microstreaming technique to isolate rare circulating cells such as circulating cancer associated fibroblasts (cCAFs) in addition to circulating tumor cells (CTCs) and immune cells ( i.e. leukocytes) from clinically diagnosed patients with a capture efficiency of 94% while preserving cell functional integrity within 8 minutes. (nsf.gov)
We employ nanoscale high resolution flow cytometry to reliably image, count, and isolate cell- and size-specific EVs in banked plasma. (selectbiosciences.com)
Connors and his team first used flow cytometry to isolate B cells from an HIV-infected donor. (the-scientist.com)

More recently, his group have introduced the technique of stroboscopic imaging flow cytometry, which allows for high resolution imaging of biological cells at throughputs in excess of 100,000 cells per second. (wikipedia.org)

Quantification of cellular and nuclear uptake rates of polymeric gene delivery nanoparticles and DNA plasmids via flow cytometry. (cbtddc.org)

Also, this combination can be mass produced very cost effectively and hundreds and thousands of these sensor systems can work in parallel, resulting in an extremely high throughput of testing. (imec-int.com)
These devices are fabricated with CMOS compatible processes, which facilitate mass production while maintaining high performance for the analysis of various cells and bionanoparticles. (fluigent.com)
The Laminar Wash AUTO 1000 System eliminates time-consuming and labor-intensive manual pipetting and handling by automating the complete surface and intracellular staining workflow for flow and mass cytometry. (curiox.com)
The Laminar Wash AUTO 1000 is a completely hands-off automated platform to dispense, fix/permeabilize, stain, wash, and transfer samples for flow and mass cytometry. (curiox.com)
High-throughput sequencing technologies, next-generation sequencing, and mass spectrometry have revolutionized genomics and proteomics research, leading to increased adoption of related tools. (globenewswire.com)
Harnessing the proprietary Starion™ mass cytometry system, Polaris Biology offers a comprehensive set of instruments, ready-to-use antibodies, customized panel kits, and informatic tools. (weeklyreviewer.com)

Technological advances over the last two decades have turned flow cytometry into a powerful high-throughput tool for a broad range of large-scale applications requiring rapid multi-parameter analysis. (labmanager.com)

Like flow cytometry, high-content imaging cytometry is multiparametric, but it requires considerable processing power for analyzing and storing large image data files, and capturing rare events is difficult. (labmanager.com)
The primary applications include broad covering liquid displays, energy, and optical lenses, but the primary startup company drive is focusing on lab-on-chip devices, biosensors, and molecular imaging systems. (edmundoptics.eu)
The Celigo is a bench-top, high throughput, micro-well plate based, bright field and fluorescent imaging system. (utsouthwestern.edu)
With the wide introduction of flow cytometry in the early 70-ies markers and subsequent cell types have evolved but it was only in the last decade that the introduction of single cell transcriptome sequencing, high dimensional cytometry and imaging cytometry started revolutionizing the way we interrogate biological samples. (frontiersin.org)
Multiline output is critical because it allows selection of the wavelength that offers the highest excitation cross section for the multiple fluorescent labels that are employed in cell imaging. (photonics.com)
At both high (x ray) and low (radio frequency [RF]) energies the body is mostly transparent, thus allowing the non-invasive imaging of the internal structure of organs and bones. (nationalacademies.org)

While it has always been ideal for multiparametric analysis of cells based on their physical characteristics and surface protein expression, flow cytometry has traditionally been too slow in processing large sample numbers to gain widespread use in high-volume applications. (labmanager.com)
As you may know, flow cytometry is counting, inspecting, and classifying cells in a fluid stream. (e-consystems.com)
Flow cytometry can be used to quantify the number and proportion of specific cell types in a heterogeneous mixture, by examining the expression of proteins often expressed on the surface of cells. (bio-rad.com)
Flow cytometry may be used to separate and sort cells based on various detectable characteristics, which has many uses in research. (bio-rad.com)
It decreased the STAT3 expression in B cell lymphoma cell lines (A20, SU-DHL-2 and OCI-Ly3), resulting in reduced proliferation of lymphoma cells featured with lower S-phase and higher apoptosis. (bvsalud.org)
The effects of doxorubicin (DOX) and paclitaxel on sheared cells using WST8 assay and stemness (CD44 + /CD24 − ) and apoptosis (Annexin V + /7‐AAD + ) using flow cytometry are tested. (nsf.gov)
Our MFCS-EZ was used to continuously inject cells and bioparticles into the micro/nanofluidic device using constant pressure. (fluigent.com)
The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
Through the intersection of biochemistry, polymer chemistry and high-precision manufacturing, we have created FlowCytes-synthetic cells that are indistinguishable from real cells. (slingshotbio.com)

Sentinel Diagnostics, an Italian company focused for more than 40 years on the development and production of in vitro diagnostics for the most advanced clinical chemistry, immunochemistry, molecular diagnostics and chromatography platforms, announced the launch of the SENTiFIT® 800 Analyser, a fully automated and high throughput system for faecal immunochemical test (FIT). (sysmex-astrego.se)

Traditional flow cytometers need to fill the sample line up to the interrogation point with material from a single sample, which is time consuming. (labmanager.com)
Conventional cytometers are effective for studying high-volume cell populations but lack spatial resolution. (e-consystems.com)
Get insights into flow cytometers and how the right cameras can greatly improve their performance. (e-consystems.com)
However, the advent of image-based flow cytometers has created the opportunity to build cutting-edge, highly productive platforms. (e-consystems.com)
In this blog, you'll get in-depth insights into how image-based flow cytometers work and why they're highly popular in modern applications. (e-consystems.com)
This makes conventional cytometers good for studying high-volume cell populations. (e-consystems.com)
Flow cytometers consist of lenses, optical filters, mirrors, prisms and other optical components to direct the light which are crucial in the successful operation of these systems. (edmundoptics.eu)

Different IDE parameters (width from 10 μm to 100 μm, gap from 5 μm to 20 μm) are also realized to assess optimum geometries for high sensitivity and SNR. (polito.it)
Later on, a similar resistive pulse sensing device was embedded into a much smaller nanochannel (nanochannel-RPS), which enabled label-free quantification of plasmid DNAs and exosomes with high sensitivity 2 . (fluigent.com)

The new high throughput analyser SENTiFIT ® 800 can meet the need of throughput, automation and accuracy in the most demanding situation. (sysmex-astrego.se)
The SENTiFIT ® 800 Analyser is a high-end system dedicated to faecal testing and able to process up to 550 tests/h, 1 million tests every year with samples in total automation and full traceability. (sysmex-astrego.se)

Lab-on-chip (LoC) devices embody several key features, including the use of small sample volume, control over fluid dynamics, easy integration of cell manipulation techniques (cell sorting, cell isolation) and higher throughput than conventional analytical methods (e.g. patch clamp) [1-2]. (polito.it)
Despite the availability of analytical methods, the difficulty in obtaining high-quality and standardized microsamples at the point of collection remains a major limitation of the process. (researchgate.net)

The proposed research and technical objectives in this project deal with computerized automatic delayering and polishing system that would be applicable to both commercial and government semiconductor device research and development with applications including Failure Analysis (FA), Fault Isolation (FI), and Reverse Engineering (RE) of semiconductor microelectronic devices. (sbir.gov)

Truly high-throughput capabilities are now possible off-the-shelf through advanced complimentary instrument and software solutions. (labmanager.com)
However, developing truly high-throughput capabilities requires the systematic removal of process bottlenecks from sample loading through data analysis. (labmanager.com)

In the fields of haematology, urinalysis, haemostasis, life science, flow cytometry, essential healthcare and now immunochemistry, we combine highly dependable, multi-functional and easy-to-operate instruments, a variety of reagents and software, plus reliable service and support. (sysmex-astrego.se)
Rapid growth in emerging economies and technological development in the urinalysis diagnostics sphere with the advent of portable and battery-operated urinalysis devices are key factors contributing to high CAGR of the market during the forecast period. (medgadget.com)
High occurrence of diabetes leads to the increasing sales of different urinalysis devices, disposable, dipsticks worldwide. (medgadget.com)

As he explained: "The objective is to combine gene marking with flow cytometry. (beckman.com)

Their submission focused on capitalizing on the power of multi-parameter flow cytometry to expand understanding of the molecular mechanisms of cancer at the single cell level. (beckman.com)
We propose to develop and test a novel marker-gene based platform for single cell tracking using multi-parameter flow cytometry. (beckman.com)

Edmund Optics offers all of the optical components you need for building medical devices such as high precision filters, lenses, mirrors, beamsplitters, polarizers, microscope objectives, and more. (edmundoptics.eu)
A Fluigent pressure-based flow controller, the MFCS™-EZ, is helping researchers from the Micro/Nano Bioelectronics and Biosensors (MBIOS) group, part of the Department of Precision Instrument Engineering of Tianjin University in China. (fluigent.com)
We can quickly scale to bioprint custom FlowCytes with extreme precision and high throughput while meeting high ISO 9001 quality standards. (slingshotbio.com)

Their research focuses on micro/nano fabricated devices and their applications for biosensors and chemical sensors. (fluigent.com)

Using the FASTAB™ microfluidic patented technology, the MFCS™-EZ generates a constant pressure-driven flow rate that allows for reliable and repeatable experiments. (fluigent.com)

Image Flow Cytometry (IFC) has been made possible by recent advancements in image technology, electronics, and digital computers. (e-consystems.com)
Flow cytometry is a key technology in the field of cancer research, but presents challenges for flow cytometry instrumentation, especially when studying solid tumors or dealing with a high volume of samples. (bio-rad.com)
It shows that the technology to establish a high-throughput vaccine formulations should target. (cdc.gov)

As a result, flow cytometry is gaining widespread use in high-throughput niches across the pharmaceutical and biotech industries and academia. (labmanager.com)
Identification of such high risk patients is very important for possible (expensive) add-on pharmaceutical therapy or the decision to operate or not. (selectbiosciences.com)
Kalorama Information, part of Science and Medicine Group, has been a leading publisher of market research in medical markets, including the biotechnology, diagnostics, medical device, and pharmaceutical industries for more than 30 years. (kaloramainformation.com)

However, the U.S. government's tax implementation on a few devices, restricted healthcare infrastructure in poor countries, and stringent regulations in procedures are obstructing the growth of the market. (medgadget.com)

In addition, Edmund Optics' portfolio comprises a range of light sources and cameras to complete your setup whether you are designing a microscope, an OCT system, or a different type of optical medical device. (edmundoptics.eu)

Integration of IFC and MEAs in the same platform enables automated, high-throughput single-cell sorting and manipulation and long-term network analysis, allowing for more comprehensive studies of cell cultures. (polito.it)
Its short processing time (10 minutes for a 10 mL sample), together with a close-to-complete CTC recovery (99.70% recovery rate) and a low WBC contamination (4.07-log depletion rate by removing 99.992% of leukocytes), results in adequate and functional CTCs for subsequent studies in the single-cell migration device. (nsf.gov)
For the first time, we employ this new approach to query CTCs with single-cell resolution in accordance with their expression of phenotypic surface markers and migration properties, revealing the dynamic phenotypes and the existence of a high-motility subpopulation of CTCs in blood samples from metastatic lung cancer patients. (nsf.gov)
Current challenges in the field are how we share these disruptive technologies to the scientific communities while still including new approaches, such as genomic cytometry and single cell metabolomics. (frontiersin.org)

Here, we detail and model a blood separation principle which exploits discrete viscosity differences caused by blood particle sedimentation in a laminar flow. (researchgate.net)

The demand for high-throughput flow cytometry solutions offering rapid multiparameter analysis is not surprising, given the context. (labmanager.com)
For three cases of liquid dripping (sequential, reverse, and delayed drippings), mixing analysis shows that the mixing efficiency increases along both mixing channel and palisade length, and is high in the middle small channel of the palisade-shape area and low on both sides. (bvsalud.org)
The Department of Ophthalmology owns two pieces of equipment that are useful for high throughput analysis. (utsouthwestern.edu)
Flow cytometric analysis demonstrated the high purity of generated microsamples. (researchgate.net)
Consequently, SYNMIKRO invested in several devices with different key aspects regarding the analysis of fluorescently-tagged molecules for its Flow Cytometry. (uni-marburg.de)

Flow cytometry is used in a variety of disciplines such as immunology, cancer, virology and molecular biology, as well as infectious disease monitoring. (edmundoptics.eu)
Capitalizing on the femtosecond field-resolving metrologies, we devise electric-field molecular fingerprinting (EMF) as a new, high-throughput analytical technique. (attoworld.de)
For more than 40 years, Sentinel Diagnostics, an Italian company based in Milan, has been developing and producing in vitro diagnostic devices for the most advanced Clinical Chemistry, Immunochemistry and Molecular Diagnostics and Chromatography platforms. (sysmex-astrego.se)

Hyperion, MIBIscope, CODEX, MACSima) and the recently evolved genomic cytometry (Citeseq or REAPseq) have enabled unprecedented insights into many biological and clinical questions, such as hematopoiesis, transplantation, cancer, and autoimmunity. (frontiersin.org)

Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput, and low-cost platform for point-of-care diagnostics. (cdc.gov)

A completely contained system for washer maintenance plates, washer load tray, heater/shaker, cooling device, reagent/buffer plates, input/output stacks and tips. (curiox.com)
The system is fed by a dedicated sample loader (Rack Handler RH-150) that provides a steady flow of up to 175 samples to the analyser unit. (sysmex-astrego.se)

The primary purpose of the ETOP is to develop and support selected new technologies that DOE JGI could establish to add value to the high throughput sequencing it currently carries out for its users. (doe.gov)

New attention towards removing the last of the bottlenecks associated with high-throughput flow cytometry has produced accessible, comprehensive commercial solutions. (labmanager.com)
The contest was designed to explore innovative research solutions involving flow cytometry . (beckman.com)

It is also available with a plate loader to facilitate the high throughput processing of samples. (beckman.com)

Complement-dependent cytotoxic crossmatch (CDCXM) and flow cytometric crossmatch (FXCM) are both common methods to predict donor-recipient compatibility - the potential success of an organ transplant. (curiox.com)

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