AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation ScienceHealth Care
Cell Culture TechniquesBatch Cell Culture TechniquesCulture TechniquesBacteriological TechniquesCulture MediaOrgan Culture TechniquesTissue Culture TechniquesCells, CulturedBacteriaEvaluation Studies as TopicBloodMicrobiological TechniquesPolymerase Chain ReactionBacteria, AerobicColony Count, MicrobialSensitivity and SpecificityFecesCentrifugationPharynxAgarBacteroidesSpecimen HandlingCell DifferentiationFungiCell SeparationReagent Kits, DiagnosticTime FactorsMethodsCell LineFluorescent Antibody TechniqueAnaerobiosisSputumBacterial InfectionsSepsisHemolysisDNA, BacterialCattleStaphylococcusCoculture TechniquesTissue EngineeringColony-Forming Units AssayCell DivisionStem CellsRNA, Ribosomal, 16SMicroscopy, ElectronWater MicrobiologyMolecular Sequence DataDNA, RibosomalImmunoenzyme TechniquesDNA PrimersBase Sequence
AssaysTissuesVaccinesBioreactorBiologyTissue cultureVivoFlow cytometryProliferationNeuronsGeneticMonoclonal antibodiesMethodsManipulateStem cellAdherentTherapiesTherapeutic2023MammalianSustainable Development GoalsVirusesCultivationEpithelialResearchEmbryosBiotechnologyMicrofluidicMeatMatricesProcessesLaboratoryDifferentiateViabilityRegenerativeTumorScientistsAntibodiesSterileTechnologyMouse embryonicDifferentiationFibroblastPrimary cultures2022IsolationDevelopmentalBioinformaticsOrganoidsSubstrateHumanMoleculesExperimentsCancerCommercializationPrimateSubstratesTypesMolecular techniquesSuspension
Assays3
  • These skills include designing and executing cell-based assays, tissue culture etiquette, and sterile techniques. (usfca.edu)
  • 4 In cell culture assays, a large proportion of the reproducibility issues come from biological variation between passages or generations of cells. (integra-biosciences.com)
  • Documented experience working with cellular and biochemical assays related to the assessment of anticancer, anti- inflammatory and anti-EMT, and ROS-related activities in live and fixed cells. (lu.se)
Tissues11
  • Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
  • Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
  • [12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
  • The entire process of going from animal tissues to cultured meat products described above is time-consuming, labor-intensive, and prone to error if done in a manual fashion. (moleculardevices.com)
  • Sampling of biological material from the lung is performed by bronchoscopy where tissues and cells from the large and from the small airways are harvested. (ki.se)
  • Furthermore, removing of cells from the tissues is possible only with the help of mechanical and enzymatic procedures before cell cultivation. (zionmarketresearch.com)
  • Cell therapies are treatments that use human cells, including stem cells, with the aim of restoring, maintaining, or improving the functioning of human tissues or organs. (eurostemcell.org)
  • These include 'adult' differentiated cells of the body (known as somatic cells, e.g. skin cells) and stem cells from a range of sources - embryonic, foetal, cord blood, and mature tissues. (eurostemcell.org)
  • Stem cell therapies hold immense promise in addressing these ailments by regenerating damaged tissues and organs. (imarcgroup.com)
  • While currently, animal cells can be produced from the starter cells in bioreactors (a scaled-up application of traditional cell culture techniques) businesses are also working to commercialize processes by which cells can be cultured using biocompatible scaffolding or other techniques to permit the formation of complex tissues, similar to strategies being explored for therapeutic organ or tissue replacement. (fda.gov)
  • Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)
Vaccines7
  • Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines . (wikipedia.org)
  • Over the years, cell culture techniques contributed to a broad spectrum of breakthrough discoveries including introduction of new vaccines and medicines. (ed.ac.uk)
  • 1 Today, cell culture has already helped countless discoveries, such as the development of vaccines against poliomyelitis, measles, mumps and other infectious diseases. (integra-biosciences.com)
  • Technological breakthroughs in proteomic gene expression and large-scale production of biopharmaceuticals & vaccines will bolster the demand for 3D cell culture systems , thereby driving the global cell culture market trends. (zionmarketresearch.com)
  • Government support for cell-based research activities and the development of cell-based vaccines will steer the expansion of the global market. (zionmarketresearch.com)
  • Because of the presence of bovine origin products in vaccines, dogs and cats develop antibodies that react with cell culture origin substrates. (vmrd.com)
  • The Expert Committee on Biological Standardization reviews developments in the field of biological substances used in human medicine, which include vaccines, plasma products and biological therapeutics. (who.int)
Bioreactor2
  • Cell cultured meat, also known as cultivated, cultured, or lab-grown meat, is a product of cellular agriculture , that uses tissue samples from living animals to grow sustainable, humane meat in a bioreactor. (moleculardevices.com)
  • Agitation based: a cell suspension is placed in a rotating bioreactor. (integra-biosciences.com)
Biology18
  • Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
  • The courses I took throughout the program, specifically Molecular Biology and Molecular Genetics and Biotechnology, have both been critical to my understanding of antibody development and characterization. (usfca.edu)
  • Core modules include Experimental Neuropathology, Cardiovascular Pathophysiology and Cancer Biology, with a range of option modules including Biocompatibility and Stem Cells and Regenerative Medicine and the Molecular Basis of Personalised Medicine. (qmul.ac.uk)
  • Experimental Pathology has given me in-depth knowledge in stem cell technology, cancer biology, neuropathology, cardiovascular pathophysiology. (qmul.ac.uk)
  • Experience in cell biology is an asset. (fems-microbiology.org)
  • As a Developmental Biology PhD student, you'll have the chance to undertake a detailed research project into the key concepts that underpin the development of an organism. (findaphd.com)
  • Studying a PhD in Developmental Biology, you'll become proficient in a range of laboratory skills, especially cell culture as well as techniques from Biochemistry, Cell Biology, and genetics. (findaphd.com)
  • It is an in vitro tool that aids the understanding of cell biology and the mechanisms of diseases. (integra-biosciences.com)
  • Development of novel treatments to control degenerative diseases requires understanding of their molecular biology. (akcchf.org)
  • a) Preference will be given to the incumbents who possess Ph D in bioinformatics, molecular biology, cell biology, biotechnology or relevant scientific discipline. (pharmatutor.org)
  • OR Research experience in molecular biology (particularly RNA biology), CRISPR-Cas9 mediated genome editing, NGS based techniques like ChIP-seq, RNA-seq, WGS. (pharmatutor.org)
  • OR (a) Preference will be given to the incumbents who possess Ph D in Life Sciences/Biotechnology/Genetics/Biochemistry/Microbiology with a minimum of 02 years of research experience (b) Experience in tissue culture, animal studies, cell signalling, Immunology, cancer biology and drug delivery, 02 years post-doc research experience in national Institutions / Universities with atleast 05 research publication in International journals. (pharmatutor.org)
  • Experience in cell culture/molecular biology techniques/next generation sequencing/microarray analysis. (pharmatutor.org)
  • 5. Conducting experiments related to cell culture, molecular biology and related techniques. (pharmatutor.org)
  • Proficiency in one or more of the following : Experience in molecular biology techniques, microbiology, biochemical techniques and imaging. (pharmatutor.org)
  • Our research programs are rooted in leveraging T cell biology to generate NKT and T cell subtypes from hematopoietic stem cells. (berkeley.edu)
  • Students will be introduced to advanced research techniques involving cell cultures, molecular biology and the use of state-of-the-art microscopes to gain first-hand biomedical research experience. (mbchamber.mb.ca)
  • Frontiers in cell and developmental biology. (lu.se)
Tissue culture7
  • However, even though cell or tissue culture methods may reduce the number of experiments performed on intact animals, the maintenance of cells in culture normally requires the use of animal-derived serum. (wikipedia.org)
  • Several tissue culture methods that measure the rate of chemical absorption by the skin have been approved by the OECD. (wikipedia.org)
  • Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
  • The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
  • In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
  • The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
  • [6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Vivo11
  • The purpose of this study is to evaluate the feasibility of human amniotic membrane (HAM) as a chondrocyte carrier by assessing cell proliferation and maintenance of phenotype in vitro and cartilage regeneration in vivo. (nih.gov)
  • 3D models provide a more accurate representation of cells in vivo and are more stable than 2D cultures, making them very useful for long-term studies. (selectscience.net)
  • Two major alternatives to in vivo animal testing are in vitro cell culture techniques and in silico computer simulation. (wikipedia.org)
  • The in vitro and ex vivo culture and maintenance of parasites involves highly complex procedures, which are subject to many variables. (hindawi.com)
  • We invite investigators to contribute original research articles as well as review articles that will motivate the continued exerted efforts in creating an understanding on the In Vitro and Ex Vivo Cultivation and Studies of Parasites and Development of Culture Media and other novel culture and maintenance conditions and applications. (hindawi.com)
  • We use cell culture techniques, mutagenesis approaches, primary cultures of neurons and glia, pharmacology, in vitro models of stroke and epilepsy, and in vivo models of neuropathic pain and cocaine abuse disorder. (drexel.edu)
  • Due to their simplicity, 2D techniques can't mimic the cells' in vivo environment, where they usually grow in three dimensional structures with complex cell-to-cell interactions. (integra-biosciences.com)
  • Since my work in the U.S was cell culture based I am now engaging in broiler trials to determine if our results were representative of satellite cell activity in vivo in commercial birds. (poultryhub.org)
  • Here, we describe the pre-clinical in vitro and in vivo study of irradiated haNK cells engineered to express a second-generation chimeric antigen receptor (CAR) targeting programmed death-ligand 1 (PD-L1). (elifesciences.org)
  • While WA/1 virus exhibited a moderately increased proportion compared to that in the inoculum following co-infection in human respiratory cells, Delta variant possessed a substantial in vivo fitness advantage as this virus becoming predominant in both inoculated and contact animals. (cdc.gov)
  • This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
Flow cytometry1
  • We collaborate with a number of highly skilled laboratories depending on our research questions, using various methods including immunohistochemistry, flow cytometry, PCR, cell culture techniques and omics methods. (ki.se)
Proliferation4
  • These cell-substrate specimens were cultured for up to 4 weeks, and cell proliferation rate and phenotypic stability were examined at weeks 1 and 4. (nih.gov)
  • The first occurs by migration and proliferation of keratinocytes from the wound edges and by differentiation of stem cells from remaining hair follicle bulbs. (worldwidewounds.com)
  • Broadly, skeletal muscle development results from proliferation and differentiation of myoblasts that fuse to form multinucleated myotubes. (poultryhub.org)
  • Post-hatch muscle hypertrophy, the subject of Dean's work, occurs through the proliferation and differentiation of myogenic satellite cells into muscle myofibres, which then fuse with existing muscle myofibres to increase their size. (poultryhub.org)
Neurons4
  • Traditional random cultures allow limited access to subcellular compartments (axons, dendrites, synapses) due to extensive and haphazard growth of neurons. (sbir.gov)
  • Our long-term goal is to provide robust, user-friendly, and cost effective culture platforms that can optically, fluidically, and biochemically access neurons and their subcellular compartments. (sbir.gov)
  • Such improvements include ensuring greater uniformity of the device, reducing end-user assembly procedures, and enhancing viability for the long-term culturing period that is often needed for neurons. (sbir.gov)
  • The significance of the proposed work is to improve research scientists' ability to visualize, manipulate, and measure cultured neurons leading to greater understanding of the underlying causes of neurological diseases. (sbir.gov)
Genetic9
  • The cells also hold valuable genetic information that could lead doctors to more informed treatment decisions and even tailor-made therapies for individual patients. (rdworldonline.com)
  • Although all cells contain the same genetic material, they develop into different directions. (mpg.de)
  • The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
  • Once they are considered a therapeutic lead, these mouse antibodies must be humanized (by genetic engineering) unless they are made in a humanized mouse, which allows more rapid progress to a potential drug stage," explains Dr. Thomas Moran, a professor of microbiology at the Icahn School of Medicine at Mount Sinai and the director of the Center for Therapeutic Antibody Development. (labmanager.com)
  • During the scale-up process, it is important to maintain productivity and genetic stability, and robustness of the cells, which requires optimization of culture conditions. (moleculardevices.com)
  • However, to realize the full potential of hiPSCs for disease modeling and drug screening, several challenges still need to be overcome, as there is the high variability between hiPSC lines, the risk of accumulating genetic aberrations when culturing hiPSCs, and the lack of standardized procedures for hiPSC generation as such (reviewed by Volpato and Webber, 2020 ). (frontiersin.org)
  • Natural killer (NK) cell-based immunotherapy may overcome genetic mechanisms of resistance to T cell-based immunotherapy through antigen- and MHC-independent recognition of malignant cells. (elifesciences.org)
  • Additionally, innovations in genetic editing techniques, like CRISPR-Cas9, offer precise control over stem cell characteristics, further expanding their therapeutic potential. (imarcgroup.com)
  • We also use human induced pluripotent stem cells to investigate how polymorphic transposable elements contribute a genetic component to these disorders, using X-linked dystonia Parkinsonism as our current focus. (lu.se)
Monoclonal antibodies1
  • Biotech products include protein drugs made by genetically-engineered cell cultures, monoclonal antibodies (laboratory-made copies of a single human antibody, often for use in cancer treatment ) and, most recently, human cell-based therapies. (eurostemcell.org)
Methods12
  • The new technique is more than three times as effective as previous methods. (rdworldonline.com)
  • It grew new cells from 73 percent of the patients in a recent study, more than three times the success rate of previous methods and a first for early-stage cancers. (rdworldonline.com)
  • Alternatives to animal testing are the development and implementation of test methods that avoid the use of live animals. (wikipedia.org)
  • In a recent paper published in the journal Nature Methods, MISpheroID investigators, including Prof Neil Carragher and Dr John Dawson from Cancer Research UK Edinburgh Centre, describe development of MISpheroID: a knowledgebase and transparency tool for minimum information in spheroid identity . (ed.ac.uk)
  • Here, we highlight various applications and research methods using our CloneSelect Imager and Single-Cell Printer, ImageXpress Cellular Imaging System, and SpectraMax® microplate reader to help scale your cell line development workflow. (moleculardevices.com)
  • Methods applied will include molecular cloning/knockout techniques, Omics-technologies, and cell culture. (fems-microbiology.org)
  • Scaffold-based 3D methods usually involve growing adherent cells in hydrogel scaffolds. (integra-biosciences.com)
  • There is considerable demand for methods to isolate synapses and there are limitations in existing techniques. (sbir.gov)
  • The need for accurate in vitro dosimetry remains a major obstacle to the development of cost-effective toxicological screening methods for engineered nanomaterial s. (cdc.gov)
  • This simple and cost-effective method allows nanotoxicologists to correctly model nanoparticle transport, and thus attain accurate dosimetry in cell culture systems, which will greatly advance the development of reliable and efficient methods for toxicological testing and investigation of nano-bio interactions in vitro. (cdc.gov)
  • Methods: This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
  • Through the direct visualization of the DNA in the nanochannels together with simple barcoding methods we can do this without any culturing of the cells nor any DNA amplification. (lu.se)
Manipulate3
  • Upon completion of this course students should be able to demonstrate the knowledge and skills required to initiate, maintain, and manipulate cells in culture. (ncbionetwork.org)
  • Adult 'differentiated' cells are easier to obtain than stem cells and the know-how to grow and manipulate them is well established, but they are mainly used in indications with relatively limited patient populations such as wound healing and repairing damaged joints and ligaments. (eurostemcell.org)
  • We therefore develop micro and nanofluidic devices on scales ranging from tens of nanometers to hundreds of micrometers to manipulate, visualize and sort biomolecules, cells and other bioparticles. (lu.se)
Stem cell20
  • One of the cell types involved in the regenerative stem cell niche are fibro/adipogenic progenitors (FAPs). (usz.ch)
  • The stem cell module was captivating and I now understand how engineered stem cells treat diseases. (qmul.ac.uk)
  • Automatically expanded hiPSCs exhibit normal growth characteristics, and show sustained expression of the pluripotency associated stem cell marker TRA-1-60 over at least 5 weeks (10 passages). (frontiersin.org)
  • These are at all stages of development from early preclinical research, as with a neural stem cell therapy being developed for Parkinson's Disease by Axontherapix S.L of Spain, through to products that are available to treat patients now such as Chondroselect ® , a cell therapy for repairing damaged tissue in knee joints produced by Belgian cell therapy firm TiGenix. (eurostemcell.org)
  • Increasing investments in stem cell research, rising chronic disease prevalence, advancements in biotechnology, collaborations between academia and industry, stringent regulations, and expanding awareness of stem cell therapies are stimulating the market growth. (imarcgroup.com)
  • Stem cell manufacturing is a highly sophisticated and rapidly evolving field dedicated to the controlled production of stem cells, which possess the unique ability to develop into umpteen specialized cell types within the human body. (imarcgroup.com)
  • There are two types of stem cell manufacturing: embryonic stem cell production, derived from early-stage embryos, and induced pluripotent stem cell (iPSC) production, which reprograms adult cells to an embryonic-like state. (imarcgroup.com)
  • The advantages of stem cell manufacturing are manifold, as it offers a renewable source of cells for research, drug testing, and potential therapeutic applications. (imarcgroup.com)
  • The global stem cell manufacturing market is influenced by increasing investments in stem cell research and regenerative medicine by governments and private organizations. (imarcgroup.com)
  • Apart from this, the increasing awareness and acceptance of stem cell-based therapies among patients and healthcare professionals is augmenting the market growth. (imarcgroup.com)
  • The global stem cell manufacturing market is experiencing a significant upsurge due to the substantial investments pouring into stem cell research and regenerative medicine. (imarcgroup.com)
  • This driver is fueled by the promising prospects of stem cell therapies in addressing previously untreatable conditions, such as neurodegenerative diseases and spinal cord injuries. (imarcgroup.com)
  • Another vital driver propelling the global stem cell manufacturing market is the escalating prevalence of chronic diseases. (imarcgroup.com)
  • Consequently, the demand for stem cell-based treatments is soaring as patients seek alternatives to traditional approaches. (imarcgroup.com)
  • This surge in demand not only drives market growth but also underscores the urgency of advancing stem cell manufacturing techniques to meet the healthcare needs of millions worldwide. (imarcgroup.com)
  • Advancements in biotechnology and cell culture techniques are revolutionizing the field of stem cell manufacturing. (imarcgroup.com)
  • This driver is pivotal in enhancing the efficiency and scalability of stem cell production. (imarcgroup.com)
  • In this project we use induced pluripotent stem cell lines from various primate species to perform direct comparative analysis in stem cell derived cultures such as cerebral organoids. (lu.se)
  • The scholarship aims to train a leading stem cell scientists, enabling significant contributions to the scientific community and advance our understanding of human airway basal stem cells in health and disease. (lu.se)
  • This is a tool to help students find the right project for their Bachelor's and Master's thesis at Lund Stem Cell Center. (lu.se)
Adherent8
  • Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)
  • There are two basic systems for growing cells: adherent and suspension cultures. (integra-biosciences.com)
  • Adherent cultures are grown on an artificial substrate, whereas cells grown in suspension are free-floating in the culture medium. (integra-biosciences.com)
  • While only a few cell types naturally grow in suspension (e.g. lymphocytes), many adherent cell types can be adapted to suspension cultures. (integra-biosciences.com)
  • There are two reasons for culturing naturally adherent cells in suspension. (integra-biosciences.com)
  • The major downside of suspension cultures is that they require daily cell counts and viability determination to follow growth patterns, whereas adherent cultures can easily be inspected under a microscope. (integra-biosciences.com)
  • Adherent cultures can be further divided into 2D and 3D cultures. (integra-biosciences.com)
  • In 2D applications, adherent cells are grown in a monolayer system on a flat surface, e.g. in a T-flask. (integra-biosciences.com)
Therapies9
  • Similarly, other modules tackle the latest developments, like targeted cancer therapies or the elasticity theory in cardiovascular aging. (qmul.ac.uk)
  • Animal cells are usually engineered to produce different biologics, including growth factors, hormones, and antibodies that are commonly used as therapies today. (moleculardevices.com)
  • There are currently around 50 firms developing cell therapies within Europe. (eurostemcell.org)
  • Companies face a choice between a range of human cell types to work with when developing new therapies. (eurostemcell.org)
  • The REMEDiE project identified some 65 cell therapies under development in the European commercial sector. (eurostemcell.org)
  • There are more cell therapies than firms because several firms have more than one product in development. (eurostemcell.org)
  • European cell therapy firms are split almost equally between therapies based on stem cells and therapies based on somatic cells. (eurostemcell.org)
  • Appia Bio is a preclinical stage biotech company founded in 2020 with the mission to bring innovative off-the-shelf cell therapies to cancer patients. (berkeley.edu)
  • Our goal is to discover and develop allogeneic cell therapies across a broad array of cancer indications with a scalability that increases access for patients. (berkeley.edu)
Therapeutic6
  • The Core offers antibody development services for preclinical antibody research and investigation, using the same process involved in therapeutic mAb development. (labmanager.com)
  • Humanization techniques are required for antibodies destined for therapeutic use in humans. (labmanager.com)
  • The main goals of my research are twofold: to investigate the regulation of EAATs in physiological states and neurological disorders, and to progress the development of compounds that target these transporters for therapeutic purposes. (drexel.edu)
  • The advent of the human induced pluripotent stem cells (hiPSCs) technology offers unprecedented opportunities for disease modeling, personalized medicine, and the development of new therapeutic interventions. (frontiersin.org)
  • The agency administers safety assessment programs for a broad array of food ingredients, including foods derived from genetically engineered plants, and also manages safety issues associated with cell culture technology in therapeutic settings. (fda.gov)
  • Identification of the latter holds promise for the development of therapeutic avenues. (lu.se)
20231
  • The global cell culture market size was evaluated at $5.55 billion in 2022 and is slated to hit $8.75 billion by the end of 2030 with a CAGR of nearly 8.37% between 2023 and 2030. (zionmarketresearch.com)
Mammalian1
  • There is an emphasis on developing your practical laboratory skills with various opportunities for hands-on experience in a range of current techniques and practices such as mammalian cell culture and fermentation. (napier.ac.uk)
Sustainable Development Goals2
  • To date, the UN General Assembly has accepted a set of 17 proposed Sustainable Development Goals as a starting point for the post-2015 agenda. (waterandhealth.org)
  • I am confident that this new collaborating centre will fulfil its mandate in supporting countries in the Region and contribute to WHO's programme of work and achievement of the Sustainable Development Goals. (who.int)
Viruses1
  • Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
Cultivation2
  • Development of serum-free media (SFM) for cultivation and studies of various parasites and cells, and in vitro identification of positive determinants of culture media and their optimal concentrations for parasites and cell growth. (hindawi.com)
  • Breakthroughs in cell culture technology, such as 3D cell culture systems and bioreactors, enable the cultivation of high-quality stem cells in larger quantities. (imarcgroup.com)
Epithelial2
  • Intact HAM was treated with 0.1% trypsin-ethylenediaminetetraacetic acid (EDTA) for 15 min and the epithelial cells removed to make a denuded HAM. (nih.gov)
  • We are looking for a highly motivated and skilled scientist with a strong background and proven track record working with human epithelial cell. (lu.se)
Research24
  • intrinsically motivated to be involved in research with interest in individual development into independence. (usz.ch)
  • Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
  • This vaccine was made possible by the cell culture research of John Franklin Enders , Thomas Huckle Weller , and Frederick Chapman Robbins , who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
  • Supervision for research was first-class and I quickly performed techniques like cell culture, Western immunoblotting, FACS. (qmul.ac.uk)
  • Human and animal cell culture techniques - growing human or animal cells under controlled conditions outside their natural environment - represent one of key technologies used in modern biomedical research. (ed.ac.uk)
  • We began work on the research and development ("R&D") element of the contract and rapidly identified our vaccine candidate, designated ACAM1000, to take forward into clinical trials. (globenewswire.com)
  • Our projects include basic research programs to further our knowledge of glutamate transporters regulation in physiological and disease states, and translational projects involving the development of small molecule allosteric modulators of these transporters. (drexel.edu)
  • Increase in the research on stem cells will proliferate the size of the global market in the years ahead. (zionmarketresearch.com)
  • Rise in the research activities on cell culture development in APAC countries is likely to offer new growth avenues for the global cell culture market in the coming years. (zionmarketresearch.com)
  • Graduates may find employment in various areas of industry and government, including research and development, manufacturing, sales, and customer service. (edgecombe.edu)
  • His research examines the impact of varied nutritional regimens on muscle development, especially focussing on the immediate post-hatch period, with the overall aim of producing broiler birds with more muscle and less fat. (poultryhub.org)
  • These countries all have strong existing infrastructures, including regulations and facilities, for biomedical research and commercialisation and so it is not surprising that they have also become 'hubs' for cell therapy within Europe. (eurostemcell.org)
  • Stringent regulatory frameworks and ethical considerations and collaborative efforts between academia and industry players for research and development (R&D) initiatives are contributing to the market growth. (imarcgroup.com)
  • These investments support cutting-edge research, the development of advanced manufacturing techniques, and the expansion of clinical trials. (imarcgroup.com)
  • Appia Bio is seeking a motivated Sr. Research Associate or Research Associate II to be part of the Process Design team with previous cell culture experience. (berkeley.edu)
  • The majority of Oxford's medical research is carried out using either in vitro techniques or the study of human beings. (ox.ac.uk)
  • We want to help Manitoba students perform well in subjects that can lead to highly skilled, cutting-edge careers in exciting areas such as medical research and development," Selinger said. (mbchamber.mb.ca)
  • DESCRIPTION (provided by applicant): Neuron-cell culture is widely used for studies in basic research, drug discovery, and toxicity testing. (sbir.gov)
  • Our course is designed to enhance your career in the medical or pharmaceutical biotechnology sectors in a variety of research, product and technology development and leadership roles. (napier.ac.uk)
  • The purpose of our research is to understand how each islet cell type and each enteroendocrine cell type in the gut is affected in type 2 diabetes. (lu.se)
  • The purpose of postdoctoral development is to promote internationalization and contribute to qualifications within research following the completion of a doctoral degree. (lu.se)
  • The primary goal of this scholarship is to foster scientific excellence and innovation by providing a conducive environment for advanced research and skill development. (lu.se)
  • Our research lies at the interface of development, ecology, and evolution. (lu.se)
  • And my luck was that I entered brain research at the time, in the late 1960s, when modern neuroscience was born, and in the labora- tory where a new groundbreaking microscopic technique, the Falck-Hillarp monoamine histofluorescence method, had just been developed. (lu.se)
Embryos7
  • Overview of Single Cell Combinatorial Indexing for labeling the transcriptome of single cells The cells of mouse embryos at day 9.5 to 13.5 were isolated and distributed into different wells of a microtiter plate. (mpg.de)
  • Together with researchers from Berlin, the American team used this method to study the activity of all cells from mouse embryos at the age of 9.5 to 13.5 days. (mpg.de)
  • The American embryologist Ross Granville Harrison developed the first in vitro cell culture technique at the very beginning of the twentieth century, when he successfully grew tissue fragments from frog embryos outside the body. (integra-biosciences.com)
  • After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
  • Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
  • Results: The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cell embryos (p=0.04). (who.int)
  • As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
Biotechnology1
Microfluidic2
  • The capture and culture process starts with a microfluidic chip device that captures cancer cells as a blood sample is pumped across it. (rdworldonline.com)
  • Organ-on-a-chip (OOC), also known as a microphysiological system, is a state-of-the-art microfluidic cell culture technology that sums up cells or tissue-to-tissue interfaces, fluid flows, mechanical cues, and organ-level physiology, and it has been developed to fill the gap between in vitro experimental models and human pathophysiology. (bvsalud.org)
Meat6
  • Cultured meat (CM) is an emerging technology, an alternative to the traditional farming industry, and crucial to supply the increasing meat demand while being more environmentally and animal friendly. (moleculardevices.com)
  • Because cell-cultured meat is made with animal cells, many processes for developing and scaling a cell-cultured meat product will mirror those in biologics production, specifically cell line development . (moleculardevices.com)
  • Is cell-cultured meat approved? (moleculardevices.com)
  • Our results so far indicate that there is potential for meat quality improvements through manipulation of satellite cell activity, and we are investigating this potential further. (poultryhub.org)
  • One such area is the development of products that are intended to resemble conventional meat, poultry and seafood. (fda.gov)
  • In either case, a significant technical challenge with respect to the use of animal cell culture technology to develop foods intended to resemble conventional meat, poultry and seafood products involves the development of the growth medium used to multiply the cells and ensure that they differentiate into the correct cell types. (fda.gov)
Matrices3
  • In conclusion, our findings suggest that denuded HAM could be one of the ideal cell carrier matrices for cartilage regeneration. (nih.gov)
  • For example, despite improvements in cell culture techniques and developments in dermal matrices, tissue-engineered skin substitutes have yet to achieve widespread use by clinicians. (worldwidewounds.com)
  • We will review cutting-edge developments and advances in the OOC realm, discussing current applications in the biomedical field with a detailed description of how this technology has enabled the reconstruction of complex multi-scale and multifunctional matrices and platforms (at the cellular and tissular levels) leading to an acute understanding of the physiopathological features of human ailments and infections in vitro. (bvsalud.org)
Processes7
  • It lacks the necessary throughput and resolution to provide a global overview of the dynamic molecular processes that take place in the different and rapidly growing populations and subpopulations of cells during organogenesis. (mpg.de)
  • Importantly, these techniques are being continuously improved to model complex biological processes taking place in our bodies. (ed.ac.uk)
  • One of the 3D cell culture techniques used nowadays to provide better insights into cancer related processes such as cancer cell migration, invasion and drug resistance are so called spheroid cultures, cancer cells grown as near-spherical multicellular aggregates. (ed.ac.uk)
  • Finally, after creation, both suspension-cultured (unstructured) and scaffold-cultured (structured) products would be further processed using traditional food manufacturing processes that fall under FDA regulations such as the Food Safety Modernization Act's preventive controls framework. (fda.gov)
  • To study processes at the level of single molecules and single cells, the tools need to be on the same size scale as the objects under study. (lu.se)
  • Using single cell RNA sequencing, we aim to identify which cellular processes are affected by type 2 diabetes and in which islet and small intestinal cell type this occurs. (lu.se)
  • We know that innate immune cells are required for blastema progenitor cell formation, but are lacking an understanding of the immune cell processes and functions that are required for inducing and maintaining these progenitors cells. (lu.se)
Laboratory5
  • Because epidemiologic investigation is increasingly dependent on molecular information, modern molecular and informational technologies must be applied to the development of useful field and laboratory deployable devices. (cdc.gov)
  • We study well characterized patient cohorts and we use various laboratory techniques, collaborating with laboratories both within Karolinska Institutet and other universities including international. (ki.se)
  • In our laboratory we have shown important changes in the mechanisms controlling cell persistence in diseased valves likely contributes to valve degeneration over many years. (akcchf.org)
  • The funding will support development of a 3,000-square-foot laboratory with classroom and study areas offering a controlled, safe and authentic teaching environment. (mbchamber.mb.ca)
  • Oman has been very supportive of regional laboratory diagnostic activities in recent years, including training on molecular techniques in RT-PCR, cell culture and virus isolation, and the sequencing of influenza and other respiratory diseases. (who.int)
Differentiate3
  • Junyue Cao, Malte Spielmann and their colleagues describe, which cell types differentiate between days 9.5 and 13.5 of mouse embryonic development, and how they transform into organs. (mpg.de)
  • Subsequently these differentiate into muscle fibres which then undergo no further cell division and can only be altered through hypertrophy. (poultryhub.org)
  • cells that behave very like embryonic stem cells and can differentiate into all the different types of cells in the body. (eurostemcell.org)
Viability2
  • The 3T3 Neutral Red Uptake (NRU) Phototoxicity Test, approved by the OECD, detects the viability of 3T3 cells after exposure to a chemical in the presence or absence of light. (wikipedia.org)
  • We'll provide you with tips on reproducibility, contamination, viability and automation to help you overcome the major cell culture challenges. (integra-biosciences.com)
Regenerative3
  • While most experiments in the 20th century were 2D-based (in 2D cultures cells are grown on a flat surface, such as the bottom of a petri dish or flask), the end of twentieth century and early twenty‐first century brought the progress in 3D cell culture technology and created the possibility of the tissue engineering and the regenerative medicine development. (ed.ac.uk)
  • Other countries such as Spain and Switzerland have made significant investments in regenerative medicine and can be considered 'emerging' or secondary sites of strength in the European cell therapy field. (eurostemcell.org)
  • This process plays a pivotal role in regenerative medicine, drug development, and disease modeling. (imarcgroup.com)
Tumor7
  • Researchers say it's a major step forward in the study of circulating tumor cells, which are shed from tumors and circulate through the blood of cancer patients. (rdworldonline.com)
  • The new capture and culture method changes this by providing a reliable way to get usable numbers of circulating tumor cells from even early-stage patients. (rdworldonline.com)
  • 3D Cell Culture: Tumor-specific cancer treatments, org. (selectscience.net)
  • mAbs are used to target and bind tumor cells expressing cancer-specific antigens and induce cell death. (labmanager.com)
  • Treatment of syngeneic tumors resulted in CD8 and PD-L1-dependent tumor rejection or growth inhibition and a reduction in myeloid cells endogenously expressing high levels of PD-L1. (elifesciences.org)
  • T cell-based immunotherapy, such as immune checkpoint blockade or adoptive T cell transfer, is limited by the ability of T cells to detect major histocompatibility complex (MHC)-presented antigen by tumor cells. (elifesciences.org)
  • Thus, addressing the presence of immunosuppressive myeloid cell populations in the periphery and tumor microenvironment of patients with cancer is likely to be required for effective NK cell-based immunotherapy. (elifesciences.org)
Scientists3
  • Scientists from Seattle and Berlin have published an atlas on mouse embryonic development. (mpg.de)
  • In total, the scientists were able to identify 38 different main cell types and over 500 subtypes. (mpg.de)
  • In order to find out which genes of a cell were active at a certain point of development, the scientists examined the transcriptome. (mpg.de)
Antibodies3
  • They covered the chip with microscopic posts that slow and trap cells, then coated it with antibodies that bind to the cancer cells. (rdworldonline.com)
  • In an early study, they fused mouse myeloma and spleen cells from an immunized donor to produce cell lines that secreted anti-sheep red blood cell antibodies. (labmanager.com)
  • They] can work by recruiting immune elements, delivering toxins, producing CAR-T cells, or as bispecific antibodies that recruit T or NK cells to kill the cancer cells. (labmanager.com)
Sterile1
  • Also, the individual should have good documentation practices and sterile lab techniques. (berkeley.edu)
Technology8
  • 3D cell culture: The technology driving advancement an. (selectscience.net)
  • Designated ACAM2000, the vaccine being developed under this contract is slightly different as it is being produced using Baxter's serum-free cell-culture technology. (globenewswire.com)
  • Conceptual understanding of theory translated to an effective application of bio-techniques (i.e. cell culture, recombinant DNA technology, bioprocess, and product development/production). (edgecombe.edu)
  • This course provides detailed knowledge of key concepts in cell technology, bioprocessing and molecular analysis and how these approaches are applied in areas of specific relevance to medical and pharmaceutical applications such as drug design and discovery, immunology and microbial infection. (napier.ac.uk)
  • In the Cell Technology module, you will be assessed on practical lab skills and also via a class test. (napier.ac.uk)
  • The use of animal cell culture technology as a method of food production and manufacturing raises many important considerations from a technical and regulatory perspective. (fda.gov)
  • Thus, as a starting point, both substances used in the manufacture of these products of animal cell culture technology and the products themselves that will be used for food are subject to the FDA's jurisdiction. (fda.gov)
  • The FDA remains committed to using our expertise in relevant scientific areas to evaluate the safety of emerging food technologies, such as foods generated by animal cell culture technology. (fda.gov)
Mouse embryonic1
Differentiation3
  • Initially, all these cells are identical, but soon, they start to form the three germ layers, which represent the first stage of differentiation of the developing embryo. (mpg.de)
  • Upon injury, FAPs enter the cell cycle and expand to produce cytokines and deposit extracellular matrix (ECM) to enhance differentiation of SCs into muscle fibers. (usz.ch)
  • It has been shown that the nutritional status of the animal can cause satellite cells to follow alternative pathways of differentiation, leading to intramuscular fat deposition and breast muscle fibre necrosis (Velleman et al. (poultryhub.org)
Fibroblast1
  • They also added cancer-associated fibroblast cells that were grown in the lab of Diane Simeone, surgical director at the Multidisciplinary Pancreatic Cancer Clinic of the U-M Cancer Center. (rdworldonline.com)
Primary cultures1
  • Leads from these studies are tested experimentally in cell models, primary cultures as well as in small and large animals. (lu.se)
20221
  • In terms of revenue, the global cell culture market size was evaluated at nearly $5.55 billion in 2022 and is expected to reach $8.75 billion by 2030. (zionmarketresearch.com)
Isolation1
  • Cell line development workflows consist of a multistep process involving single-cell cloning, formation and isolation of these discrete colonies, and the phenotypic characterization of these colonies. (moleculardevices.com)
Developmental1
  • In addition, they described 56 developmental trajectories in organ development for different cell types. (mpg.de)
Bioinformatics1
  • Prior experience with Bioinformatics and Sequencing techniques is a plus. (usz.ch)
Organoids1
  • Our lab has developed advanced bioengineering techniques offering the generation of human organoids. (lu.se)
Substrate1
  • Everything from how single cells in culture respond to their substrate to the way whole organ systems develop and respond to environmental stress. (lu.se)
Human5
  • This vector efficiently produced Tet-ON regulatable immortalized (293T) and primary (human mesenchymal stem cells and human primary fibroblasts) cells. (nih.gov)
  • EpiDerm from Mattek and EpiSkin and SkinEthic RHE model are derived from human skin cells which have been cultured to produce a model of human skin. (wikipedia.org)
  • Here, we demonstrate that NK cells (haNKs) engineered to express a PD-L1 chimeric antigen receptor (CAR) haNKs killed a panel of human and murine head and neck cancer cells at low effector-to-target ratios in a PD-L1-dependent fashion. (elifesciences.org)
  • At these levels and everything in between, work is carried out using an enormous range of approaches such as gene arrays, molecular techniques, cell cultures, tissue samples, computer models, animal studies, and human studies. (ox.ac.uk)
  • The development of the human blood-CSF-brain barrier. (cdc.gov)
Molecules2
  • Subsequently, all cell nuclei were extracted and a molecular barcode specific to the respective embryo was introduced to the RNA molecules of each cell. (mpg.de)
  • These antibiotics attack fat molecules in bacteria cell walls. (waterandhealth.org)
Experiments5
  • However, some claim they are not true alternatives because simulations use data from prior animal experiments and cell cultures often require animal derived products, such as serum or cells. (wikipedia.org)
  • 1,500 3D spheroid experiments and provides a recommended minimum information for spheroid culture. (ed.ac.uk)
  • This is why some experiments are conducted using 3D cultures, which can be grown using scaffold-based or scaffold-free techniques. (integra-biosciences.com)
  • Despite the different approaches and techniques, all experiments have one thing in common: it's difficult to grow viable cells in the desired quantity to obtain reproducible results. (integra-biosciences.com)
  • These experiments are closely linked to epigenetic analysis and gene editing experiments performed in cell culture models of glioma. (lu.se)
Cancer14
  • In a development that could lead to a deeper understanding of cancer and better early-stage treatment of the disease, University of Michigan researchers have devised a reliable way to grow a certain type of cancer cells from patients outside the body for study. (rdworldonline.com)
  • But progress has been slow, largely because the cells are rare in early-stage cancer patients. (rdworldonline.com)
  • This culture method gives clinicians a way to study each patient's cancer much earlier and much more frequently," Nagrath said. (rdworldonline.com)
  • The technique may also bring doctors closer to their goal of capturing cancer cells for diagnosis with a quick, non-invasive "blood biopsy" instead of the tissue biopsies that are currently used. (rdworldonline.com)
  • After the cancer cells were captured on the chip, the team pumped in a mixture of collagen and Matrigel growth medium. (rdworldonline.com)
  • The captured cancer cells prospered in the mixture, reproducing additional cells in 73 percent of tested samples. (rdworldonline.com)
  • Primary cancer cells don't grow well on a flat surface, and like people, they need neighbors to really prosper," Nagrath said. (rdworldonline.com)
  • The collagen and Matrigel provide a three-dimensional environment for the cells to grow, while the cancer-associated fibroblasts give them the neighboring cells they need. (rdworldonline.com)
  • Cancer cells change constantly and they can quickly develop resistance to a given treatment," Wicha said. (rdworldonline.com)
  • Different types of cancer cells grown as spheroids in various cell culture media (for details see Peirsman et al. (ed.ac.uk)
  • Humanization techniques have moved mAbs into the clinic, and several are FDA-approved for use as cancer therapeutics. (labmanager.com)
  • Monoclonals can be used directly to bind to surface proteins on cancer cells to kill or inhibit their growth," explains Dr. Moran. (labmanager.com)
  • PD-L1 CAR haNKs reduced levels of macrophages and other myeloid cells endogenously expressing high PD-L1 in peripheral blood from patients with head and neck cancer. (elifesciences.org)
  • The individual will be part of a team to enable the development of a groundbreaking allogeneic iNKT platform to treat cancer. (berkeley.edu)
Commercialization2
  • Prior to commercialization, mAb development begins with cell culture for cell line generation. (labmanager.com)
  • Molecular Devices has a history of success in scaling biologics for regulatory approval and commercialization with life science groups worldwide, and can leverage that expertise with food manufacturers growing animal cell lines in new industries. (moleculardevices.com)
Primate1
  • EVs cause cytopathic effects in primate cell cultures, although not initially associated with any disease condition. (medscape.com)
Substrates1
  • While chondrocytes grew in monolayer fashion on the surface of IHE and DHB substrates, the cells seeded in DHS penetrated and spread into the whole thickness of the stromal layer. (nih.gov)
Types2
  • No relationship was found between nkMT distribution and spindle elongation, and the several different nkMT distributions which have been reported for other cell types may be variations on a structural theme. (vuw.ac.nz)
  • Muscle regeneration is characterised by a well-timed network of different cell types providing an environment that allows the activation of muscle stem cells, called satellite cells (SCs) to regenerate the damaged tissue. (usz.ch)
Molecular techniques1
  • Preference will be given to applicants with experience in microbiological and molecular techniques. (fems-microbiology.org)
Suspension6
  • The AGE1.CR.pIX cell line grows in true suspension and has been optimized for viral vaccine production and stability. (b3cnewswire.com)
  • The first advantage of suspension cultures is that it's easier to passage the cells, as you don't need to detach them from a culture vessel by enzymatic or mechanical dissociation. (integra-biosciences.com)
  • Secondly, suspension cultures are easier to scale up, as the cell growth is only limited by their concentration in the medium, not by the available surface area. (integra-biosciences.com)
  • Forced-floating: a cell suspension is loaded into the wells of a low adhesion polymer-coated microplate. (integra-biosciences.com)
  • Hanging drop: a cell suspension is loaded into the wells of a hanging drop plate. (integra-biosciences.com)
  • An important key to accurate in vitro dosimetry is the characterization of sedimentation and diffusion rates of nanoparticle s suspended in culture media, which largely depend upon the effective density and diameter of formed agglomerates in suspension. (cdc.gov)