Determinants tumor cells cultured. Medical search. Frequent questions

The PI3K pathway is considered to be a major determinant of the glycolytic phenotype through AKT1 and mTOR signaling, and subsequent downstream Hypoxy Inducible Factor 1 (HIF-1) transcription factor activation. (sigmaaldrich.com)
They identified CEA expression as a major determinant of cibisatamab sensitivity as only cell lines expressing moderate to high CEA levels were susceptible to T cell mediated killing," the researchers commented. (genengnews.com)
These results indicate that BiP is a major determinant of resistance to Ad-REIC-induced apoptosis. (elsevierpure.com)
Histidine catabolism is a major determinant of methotrexate sensitivity. (harvard.edu)

The coagulation system constitutes an important facet of the unique vascular microenvironment in which primary and metastatic brain tumors evolve and progress. (rmmj.org.il)

Conversely, the coagulant microenvironment may also influence the molecular evolution of brain tumor cells through selective and instructive cues. (rmmj.org.il)
1998. Structural cues from the tissue microenvironment are essential determinants of the human mammary epithelial cell phenotype. . (ucsf.edu)
1996. The importance of the microenvironment in breast cancer progression: recapitulation of mammary tumorigenesis using a unique human mammary epithelial cell model and a three-dimensional culture assay. . (ucsf.edu)
An immune cell in the tumor microenvironment that may be important for inhibiting the immune response against bladder cancer is the myeloid derived suppressor cell (MDSC). (iospress.com)
Our group is studying interactions within the microenvironment of solid tumors that cause immune-suppression and tumor immune evasion. (cemm.at)
What factors drive T cell exhaustion in the tumor microenvironment and the draining lymph node? (cemm.at)
The microenvironment of solid tumors is characterized by a multitude of immune-suppressive factors, such as cytokines, high apoptotic cell load, unique extracellular matrix composition, hypoxia, scarcity of nutrients, metabolic waste products, and others. (cemm.at)
In addition to cancer cells, various immune cell subsets take part in shaping the suppressive tumor microenvironment, which in turn locally drives cancer-associated immune-regulatory phenotypes of monocytes, macrophages, fibroblasts and dendritic cells, often coupled with regulatory T cells and exhausted T cells that are unable to mount tumor-directed cytotoxic responses. (cemm.at)
Tumor-directed T cell responses are a prerequisite for successful immunotherapy, however, often T cells have an exhausted phenotype in the tumor microenvironment and fail to eliminate tumor cells. (cemm.at)
A key cause of this immune insensitivity is the hostile solid tumor microenvironment (TME) dominated by immunosuppressive myeloid cells. (ox.ac.uk)

In this report, the biologic functions of MDSC are defined and evidence linking MDSC with the response to cancer immunotherapies in solid tumors are reviewed. (iospress.com)

The fundamental tenet of precision oncology defines molecular characterization of tumors to guide optimal patient-tailored therapy. (korea.ac.kr)
Compared with the traditional long-term cultured cancer cell line models, PDCs recapitulate the molecular properties and biology of the diseases more precisely. (korea.ac.kr)
Here, we provide insights into dynamic pharmacogenomic associations, including molecular determinants that elicit therapeutic resistance to EGFR inhibitors, and the potential repurposing of ibrutinib (currently used in hematological malignancies) for EGFR-specific therapy in gliomas. (korea.ac.kr)
Using miniature laboratory-grown tumors known as patient-derived organoids (PDOs) as models of CRC, the researchers also showed how expression of the immunotherapy target could be reactivated by blocking a particular molecular pathway, and so potenitally resensitize the tumors to treatment. (genengnews.com)
While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. (mdpi.com)
In this regard, the emerging molecular diversity of brain tumor suptypes (e.g. in glioma and medulloblastoma) highlights the link between oncogenic pathways and the tumor repertoire of coagulation system regulators (coagulome). (rmmj.org.il)
We suggest that effective targeting of the coagulation system in brain tumors should be explored through molecular stratification, stage-specific analysis, and more personalized approaches including thromboprophylaxis and adjuvant treatment aimed at improvement of patient survival. (rmmj.org.il)
A team led by Professor Lorna Harries, Professor of Molecular Genetics at the University of Exeter, has discovered a new way to rejuvenate inactive senescent cells. (sciencedaily.com)
We describe an RNA interference (RNAi) screen for tumor-based molecular determinants of sensitivity to cetuximab-mediated ADCC. (researchassistantresume.com)
Our studies, supported by the Liddy Shriver Sarcoma Initiative, aimed to identify unique miRNA expression profiles of WDLPS and DDLPS with the hope that such signatures could provide insights into our understanding of the molecular determinants driving these two entities. (sarcomahelp.org)
Our ultimate goal is to identify cellular, molecular and spatial determinants that stimulate or suppress human tumor immunity and to target suppressive phenotypes, while enabling effector populations. (cemm.at)
This was followed by molecular profiling of the retrieved individual cells, allowing the assessment of the correlation between phenotype distribution of intercellular interactions and their genetic bases. (nature.com)
Our novel gene discoveries have led to cellular and molecular studies on topics such as cell division, cytokinesis, neuron polarization, and axon guidance. (virginia.edu)
Using cell biological, genetic, and genomic approaches, we will rigorously dissect the primary effects of abscission dysregulation on cell polarity and fates, as well as the molecular pathways activated. (virginia.edu)
Molecular determinants of outcomes in meningiomas. (moffitt.org)
With a industrial web at Princeton, Cell, Developmental and Cancer Biology welcomes amount cells from the Departments of Molecular Biology, Engineering, Physics and Genomics that owe a new doubt in funneling courses of major , direct factors and page. (cutechabeads.com)
H- ras was one of the oncogenes that first caught the attention of molecular biologists interested in cell signaling, cell growth control, and cancer. (medscape.com)

We investigated the potential of TP53 status to be a determinant of the bi-faceted role of ESR2 and associated therapeutic implications for triple negative breast cancer (TNBC). (nih.gov)

Therefore, a high rate of glucose uptake is required to meet increased energy needs to support rapid tumor progression. (sigmaaldrich.com)
Purpose: Hypoxia-inducible factor 1 (HIF-1) promotes cancer cell survival and tumor progression. (lincoln.ac.uk)
The reasons for this are multiple and include the variable and often long times to progression, the fact that direct measurements on target tumors are often not possible, and also include patient- intrinsic effects related to both patient and tumor heterogeneity. (biomedcentral.com)
While the immediate medical needs often frame the discussion on current clinical challenges, the coagulation pathway may contribute to brain tumor progression through subtle, context-dependent, and non-coagulant effects, such as induction of inflammation, angiogenesis, or by responding to iatrogenic insults (e.g. surgery). (rmmj.org.il)
miRNA deregulation has been identified in a variety of epithelial origin cancers, where changes in specific miRNAs expression possibly contribute to tumor growth, progression, metastasis, and chemoresistance. (sarcomahelp.org)
For those who are ineligible for cisplatin-based regimens or experience progression of disease, in 2016-2017 the United States Food and Drug Administration approved five monoclonal antibodies that achieve immune checkpoint blockade by targeting the programmed cell death protein-1/programmed death-ligand 1 (PD-1/PD-L1) pathway. (iospress.com)
Glazar DJ, Grass GD, Arrington JA, Forsyth PA, Raghunand N, Yu HM, Sahebjam S, Enderling H. Tumor Volume Dynamics as an Early Biomarker for Patient-Specific Evolution of Resistance and Progression in Recurrent High-Grade Glioma. (moffitt.org)
Figure 2 MiR-20a restoration in HCC cell lines inhibit proliferation and block cell cycle progression in vitro (A) and (B) Validation of miR-20a level in SMMC-7721 and Methane monooxygenase HepG2 cells upon transfection with miR-20a precursor. (aktsignals.com)
D) and (E) Influence of miR-20a on cell cycle progression of HCC cell lines. (aktsignals.com)
Our data provide evidence that estrogen signaling mediates the tumor vasculogenic process required for breast cancer progression and involves a key regulator of the hypoxia signaling pathway. (biomedcentral.com)
At the same time, it is well recognized that the host immune system is an important determinant of the clinical presentation of active TB disease, and patients with an immature or suppressed immune system often have faster disease progression and more disseminated disease [ 6 - 10 ]. (cdc.gov)

Transcriptional effects on TP53-target genes and cell proliferation in response to knocking down or overexpressing ESR2 were determined. (nih.gov)
AMPK is often considered a metabolic checkpoint as it can control cell proliferation when activated under energetic stress, and activation of AMKP results in the inhibition of mTOR activity. (sigmaaldrich.com)
2009. Understanding the Warburg Effect: The Metabolic Requirements of Cell Proliferation. (sigmaaldrich.com)
knockdown also reduced cell proliferation impartial of its ADCC enhancement effects. (researchassistantresume.com)
G-MDSC are also referred to as polymorphonuclear (PMN)-MDSC, which differ from conventional neutrophils via expression of lectin-type oxidized LDL receptor 1 (LOX-1), which inhibits T cell proliferation [ 7, 8 ]. (iospress.com)
Now, we are investigating how MESH1 removal leads to proliferation of arrests and anti-tumor phenotypes. (duke.edu)
Deletions of multiple GID subunits compromise cell proliferation, and this defect is accompanied by deregulation of critical cell cycle markers such as the retinoblastoma (Rb) tumor suppressor, phospho-Histone H3 and Cyclin A. We identify the negative regulator of pro-proliferative genes Hbp1 as a bonafide GID/CTLH proteolytic substrate. (elifesciences.org)
Table 3) significantly affected RFS of HCC patients following LT. Effects of miR-20a restoration on HCC cell proliferation and cell cycles in vitro Cell proliferation is a GSK2245840 concentration key determinant of tumor malignancy. (aktsignals.com)
However, the association of miR-20a with HCC cell proliferation is unknown. (aktsignals.com)
effects of miR-20 restoration were detected by Taqman qPCR prior to the proliferation assay (Figure 2A and B). In cell proliferation assay, the proliferation rate was suppressed in HepG2 and SMMC-7721 cells after transfection with miR-20a precursor, and the inhibitory efficiencies were 41.3% and 39.0%, respectively (Figure 2C). (aktsignals.com)
C) Proliferation assay of HCC cell lines in response to miR-20 restoration. (aktsignals.com)
Cell proliferation assay was done after culturing for 72 h. (aktsignals.com)
Therefore, the MAPK family governs almost all the physiological functions and processes such as cell adaptation, proliferation, differentiation, survival, and programmed cell death. (spandidos-publications.com)

Importantly, MESH1 removal also triggers proliferative arrest and other robust anti-tumor effects. (duke.edu)
In mice, genetic deletion of Gpr65 or oral administration of small molecule GPR65 inhibitors in vivo causes a profound repolarization of immunosuppressive tumor associated macrophages, an increase in infiltrating effector cells and potent anti tumor effects in syngeneic models. (ox.ac.uk)

Cutaneous tumors encompass a vast array of malignancies, ranging from neuroendocrine to lymphoid in origin. (medscape.com)
The diagnosis and treatment of these cutaneous tumors is constantly evolving, and the role of clinicians relies on accurate diagnosis and a thorough understanding of the clinical sequelae. (medscape.com)
The origin of cutaneous tumors can be simplistically divided into three major categories: epidermal, dermal, and adnexal (see image below). (medscape.com)
The treatment and diagnosis of many cutaneous tumors is continually changing, and the role of clinicians (eg, primary care physicians, dermatologists, plastic surgeons, pathologists) focuses on an accurate diagnosis and a thorough understanding of the clinical sequelae. (medscape.com)
In addition, these rare cutaneous tumors are often difficult to diagnose based solely on the physical examination findings, and the proper diagnosis relies on excisional biopsy with histologic studies. (medscape.com)

Here we show that dietary fructose improves the survival of intestinal cells and increases intestinal villus length in several mouse models. (nature.com)
The ability of fructose to promote cell survival through an allosteric metabolite thus provides additional insights into the excess adiposity generated by a Western diet, and a compelling explanation for the promotion of tumour growth by high-fructose corn syrup. (nature.com)
Fig. 2: Fructose metabolism enhances hypoxic cell survival and decreases pyruvate kinase activity. (nature.com)
Therapy using checkpoint inhibitor immunotherapies can increase survival in some CRC patients with hypermutated microsatellite instability (MSI) tumors, but are ineffective against the majority of cases, including microsatellite stable (MSS) CRC. (genengnews.com)
ADCC, ADCP (1, 2, 3)), as well as for driving the internalization of receptors linked to tumor survival (e.g. (news-medical.net)
Integrins and cell adhesions as modulators of mammary cell survival and transformation. (ucsf.edu)
2-5 Long term survival of patients with WDLPS is common in that these tumors lack metastatic potential, whereas DDLPS are markedly aggressive and distant metastases and/or multi- focal recurrence followed by death constitutes a typical clinical course. (sarcomahelp.org)
In TCGA data, across all tumors, patients homozygous for a hypomorphic coding variant in GPR65 (I231L) show increased overall survival, providing compelling genetic evidence of the clinical potential of GPR65 inhibition. (ox.ac.uk)
I will investigate folate utilization by various cell types and tissues and explore the cancer-specific survival mechanisms of folate-deprived cells in culture and in vivo. (harvard.edu)
In addition, the addition of VEGF to the culture medium can increase the survival rate of neurons in the cerebral cortex as well as the number and length of ganglion cell processes, to promote nerve growth. (spandidos-publications.com)

Our results support WRN as a promising synthetic-lethal target in dMMR/MSI-H colorectal cancer tumors as a monotherapy or in combination with targeted agents, chemotherapy, or immunotherapy.This article is highlighted in the In This Issue feature, p. 1861. (bvsalud.org)
Scientists at The Institute of Cancer Research and the Royal Marsden NHS Foundation Trust in the U.K. have found that colorectal cancer (CRC) tumors can switch off expression of a cell surface molecule that is a key target of immunotherapy, which stops the treatment from working. (genengnews.com)

The CSCs also possess characteristics similar to normal stem cells such as differentiation into various cell types. (ufluidix.com)
Furthermore, they could show the cross-tissue differentiation capability of these cancer stem cells into various lineages such as endothelial and cardiac. (ufluidix.com)
The functions of gangliosides as specific determinants suggest its important role in the growth and differentiation of tissues as well as in carcinogenesis. (wikipedia.org)
It has been found that tumor formation can induce the synthesis of a new complement of ganglioside, and very low concentrations of a specific ganglioside can induce differentiation of cultured neuronal tumor cells. (wikipedia.org)
and what stages of T cell differentiation/maturation are targeted. (cemm.at)
I would no avoid to learn my NRE cells to RFC as they are lower process of differentiation and no affect overcome a planning page. (cutechabeads.com)

BACKGROUND: Checkpoint inhibitor (CPI) immunotherapies have provided durable clinical responses across a range of solid tumor types for some patients with cancer. (bvsalud.org)
Cibisatamab has shown promising results in early clinical trials, but some tumors are resistant to therapy, and studies in cancer cell lines have suggested that the effectiveness of cibisatamab is dependent upon expression of CEA on the tumor cells. (genengnews.com)
Clinical sam- of ALV-J propagated in the DF-1 cells. (cdc.gov)
Data from recent clinical trials have highlighted the potential for this therapeutic modality to effect potent anti-tumor activity. (biomedcentral.com)
Pre-clinical evidence suggests that MDSC suppress anti-tumor immune responses. (iospress.com)
Typically, the clinical presentation of clear cell acanthoma is a solitary nodule in the lower extremities. (medscape.com)

In contrast, irradiation of C6 or HN5 reporter xenografts with 8 Gy in vivo elicited marked upregulation of HIF-1 signaling and downstream proangiogenic signaling at 48 hours which preceded recovery of tumor growth. (lincoln.ac.uk)
Down-regulation of BiP with siRNA sensitized the resistant cells to Ad-REIC in vivo as well as in culture. (elsevierpure.com)
The concept of enhancing cellular immunity through the transfer of ex-vivo expanded T cells was pioneered by Greenberg et al. (biomedcentral.com)
Both in vitro and in vivo data showed overexpression of plakoglobin in both the cell membrane and the cytoplasm of IMPC clusters. (jcancer.org)
Using mouse models, we validated the metastatic potential of tumor clusters was higher than single cells in vivo . (jcancer.org)
1997. Reversion of the malignant phenotype of human breast cells in three-dimensional culture and in vivo by integrin blocking antibodies. . (ucsf.edu)
To achieve our goals, we combine cell-culture based high-throughput technologies with mechanistic in vivo studies, single-cell transcriptomics and immune-profiling of patient-derived tumor material. (cemm.at)
Consistent with these findings, in vivo administration of a small molecule GPR65 inhibitor elicited similar changes in human CA9 expressing RCC PDX tumors implanted in myeloid boosted CD34+ stem cell engrafted NCG mice. (ox.ac.uk)
By studying the brain phenotypes and the cellular roles of the encoded proteins, with both in vivo and in vitro experiments, we hope to understand how small changes at the single-cell level can cause dramatic changes in overall brain structure. (virginia.edu)
Building on these exciting discoveries, we propose to elucidate the in vivo roles of abscission regulation in neural stem cell fates and proper corticogenesis. (virginia.edu)
Previously we found that estrogen aided in this neovasculogenesis via recruitment of bone marrow derived endothelial progenitor cells (BM-EPCs), leading to increased vessel formation and vascular endothelial growth factor (VEGF) production in vivo . (biomedcentral.com)

In order to distinguish between KA and SCC, further histologic studies can be used, including proliferating-cell nuclear antigen immunostaining. (medscape.com)
this is a heterogeneous group of tumors composed of several histologic subtypes. (sarcomahelp.org)

Mutations in tumor suppressor genes such as LKB1, involved in AMPK activation, have been identified in certain cancers. (sigmaaldrich.com)
In oral SCC, modern DNA technology, especially allelic imbalance (loss of heterozygosity) studies, have identified chromosomal changes suggestive of the involvement of tumor suppressor genes (TSGs), particularly in chromosomes 3, 9, 11, and 17. (medscape.com)

Using 4-hr 51 Cr release assays, pool-sensitized effector cells lysed autologous tissue-cultured tumor and fibroblast targets in 4 of 4 experiments. (johnshopkins.edu)
Fresh tumor cells, PBL, and Con A blasts appeared equally lysable, since they were equally lysed by effector cells specifically sensitized to alloantigens present on these cells. (johnshopkins.edu)
and antibody with effector cells. (researchassistantresume.com)
Antibody was added at concentrations and effector cells were added at effector-to-target ratios (E:T) indicated and incubated for 4 h. (researchassistantresume.com)
Specific lysis was determined for antibody-independent natural cytotoxicity (NK92-CD16V cells only) as refers to the plated target cells refers to effector cells and refers to the monoclonal antibody. (researchassistantresume.com)

Here, we present the potential of tumor organoids, three-dimensional cultures of tumor cells, to model GEP-NEC. (bvsalud.org)

Human lymphocytes sensitized in vitro to allogeneic single-donor cells as well as to pools of allogeneic donor cells were evaluated for their cytotoxic potential against autologous fresh solid tumor cells, autologous tissue-cultured tumor and fibroblasts, fresh peripheral blood lymphoid cells (PBL), and Con A blasts of PBL. (johnshopkins.edu)
Cold target inhibition studies demonstrated that cultured tumor and fibroblasts but not PBL shared the determinants recognized by pool-sensitized cells. (johnshopkins.edu)
Firstly, we could confirm that transforming growth factor β1 (TGFβ1) secreted from breast tumor cells is a paracrine mediator of tumor-stroma interaction leading to the activation of autophagy in the stroma component fibroblasts. (nature.com)
Through proof of concept experiments using TGFβ1 as a model factor, we could demonstrate real time monitoring of autophagy induction in fibroblasts by single tumor cells. (nature.com)
In this study, MDA-MB-231 (MDA) triple-negative breast carcinoma cells were used as a tumor cell model and mouse fibroblasts expressing an autophagy marker protein called GFP-LC3, were used as a stroma model. (nature.com)

Although this pathway has been implicated in obesity and tumour promotion, the exact mechanism that drives these pathologies in the intestine remains unclear. (nature.com)
Measuring protein phosphorylation and expression in glioblastoma cells across 40 signaling pathway nodes in response to different drugs and for different oxygen tensions revealed that SHP2 antagonism has network-level, context-dependent signaling consequences that affect cell phenotypes (e.g., cell death) in unanticipated ways. (aacrjournals.org)
Targeted disruption of HIF-1 signaling established this pathway to be a determinant of tumor radioresistance. (lincoln.ac.uk)
we have found the Hippo pathway effectors TAZ and YAP are responsible for the cell density-dependent ferroptosis (4, 6, 7). (duke.edu)
Estrogen modulation of HIF-1 and subsequent effects on endothelial cells is dependent on the Akt/PI3K pathway and protein synthesis as validated by the use of the inhibitors wortmannin and cycloheximide which abrogated estrogen's effects respectively. (biomedcentral.com)

The specific role played by HIF-1 and tumor - stromal interactions toward determining tumor resistance to radiation treatment remains undefined. (lincoln.ac.uk)
High-resolution positron emission tomography and dual-contrast DCE-MRI of immobilized dorsal skin window tumors confirmed postradiotherapy HIF-1 signaling to spatiotemporally coincide with impaired stromal vascular function. (lincoln.ac.uk)
Conclusions: Our results illustrate that tumor radioresistance is mediated by a capacity to compensate for stromal vascular disruption through HIF-1 - dependent proangiogenic signaling and that clinically relevant vascular imaging techniques can spatially define mechanisms associated with tumor irradiation. (lincoln.ac.uk)
Tumour-stromal interactions. (ucsf.edu)
As a result, the functionality of many different immune and stromal cell populations is compromised, and tumor immune evasion is facilitated. (cemm.at)
We used this system to study how tumor cells induced autophagy in the stromal niche. (nature.com)

This relationship may influence the mechanisms of spontaneous and therapeutically provoked tumor cell interactions with the coagulation system as a whole. (rmmj.org.il)
1998. Reciprocal interactions between beta1-integrin and epidermal growth factor receptor in three-dimensional basement membrane breast cultures: a different perspective in epithelial biology. . (ucsf.edu)
Retrieval of individual tumor cells from the microfluidic system and their subsequent genomic analysis was possible, allowing us to determine the nature of the factor mediating tumor-stroma interactions. (nature.com)
Therefore, our microfluidic platform might be used as a promising tool for quantitative investigation of tumor-stroma interactions, especially for and high-throughput screening of paracrine factors that are secreted from heterogeneous tumor cell populations. (nature.com)
However, conventional tools used to study cell-to-cell interactions only deliver averaged information from a population of cells and fail to provide information on the distribution of responses reflecting the heterogeneity of individual cells. (nature.com)
Cell-to-cell interactions may also be studied at a single-cell level. (nature.com)
However, to the best of our knowledge, single-cell-based techniques have been rarely used for studying the interactions of tumor cells with tissues surrounding them, i.e., the stroma. (nature.com)
In this study, we designed a biochip system that allows the time-course measurement of cancer cell-stroma interactions at a single-cell level. (nature.com)

Imatinib inhibition of c-Abl kinase activity also enhanced ADCC - phenocopying knockdown - against several EGFR-expressing head-and-neck squamous cell carcinoma (HNSCC) cell lines by main NK cells. (researchassistantresume.com)
It and the gene for the epidermal growth factor receptor ( EGFR ) are involved in cell signaling. (medscape.com)

In these cultures, GPR65 inhibition caused a dose dependent suppression of a geneset closely overlapping with that modulated by GPR65 in primary macrophages. (ox.ac.uk)

Similarly, in 2 of 2 experiments with single-donor allosensitized effectors, autologous tissue-cultured tumor was lysed. (johnshopkins.edu)
While brain tumor cells express tissue factor (TF) and other effectors of the coagulation system (coagulome), their propensity to induce local and peripheral thrombosis is highly diverse, most dramatic in the case of glioblastoma multiforme (GBM), and less obvious in pediatric tumors. (rmmj.org.il)
From a physiological standpoint, MDSC can be thought of as effectors of a homeostatic mechanism that regulate T cell-mediated inflammatory responses to pathogens [ 5 ]. (iospress.com)

Rapid breast tumor development relies on formation of new vasculature to supply the growing malignancy with oxygenated blood. (biomedcentral.com)

Model-generated hypotheses were validated in multiple glioblastoma cell lines, in mouse tumor xenografts, and through analysis of The Cancer Genome Atlas data. (aacrjournals.org)
Cancer stem cells (CSCs) are a small subpopulation (often 1-3%) within the tumor and are capable to regenerate malignant cells. (ufluidix.com)
Therefore, a better understanding of these cancer stem cells, also called tumour-initiating cells, can lead to developing more effective cancer therapy methods and formulations. (ufluidix.com)
Recently, in a study published in the Advanced Science journal, a group of researchers has employed microfluidics and reported a label-free microfluidic chip to isolate and culture cancer stem cells. (ufluidix.com)
Our one-single-cell microencapsulation approach for CSC isolation and culture may be valuable for understanding cancer biology and etiology and for facilitating the development of CSC-targeted effective therapies to fight against cancer. (ufluidix.com)
The research team took a bioinspired approach for isolation and culturing the cancer stem cells. (ufluidix.com)
Inspired by colony formation in prehatching embryos, they generated core-shell droplets (double emulsions) to encapsulate cancer cells. (ufluidix.com)
They used a microfluidic droplet generator chip to encapsulate the cancer cells in an alginate-based hydrogel core (~206 µm). (ufluidix.com)
By observing the capability of colony formation of the cells, they concluded that the cancer stem cells take up ~3-5% of the cancer cell population. (ufluidix.com)
Moreover, RNA sequencing of the colonies confirmed the stemness of the cancer stem cells. (ufluidix.com)
The droplet microfluidic approach was promising in isolating and analysis of the cancer stem cells. (ufluidix.com)
Learn more about the techniques used in microfluidics for isolating circulating tumor cells (CTCs) as potential determinants of cancer prognosis. (ufluidix.com)
Towards this goal, we have established a compilation of pharmacological landscapes of 462 patient-derived tumor cells (PDCs) across 14 cancer types, together with genomic and transcriptomic profiling in 385 of these tumors. (korea.ac.kr)
Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not. (sigmaaldrich.com)
2011. Regulation of cancer cell metabolism. (sigmaaldrich.com)
Colour-enhanced image of human colon cancer cells in culture. (genengnews.com)
Cibisatamab (CEA-TCB) is a novel bispecific antibody immunotherapy that effectively brings the cancer cells and cancer-killing T cells of the immune system together. (genengnews.com)
The other antibody arm binds to T cells that kill the cancer cells. (genengnews.com)
They also found that, unlike cancer cell lines, PDOs were capable of exhibiting mixed CEA expression, and these showed partial response to cibisatamab. (genengnews.com)
Taken together, CEA expression was frequently heterogeneous, showing a bimodal pattern in 50% of PDOs which has not been observed in cancer cell lines, and this phenomenon was associated with poor susceptibility to cibisatamab treatment," the researchers stated. (genengnews.com)
Interestingly, when single CEA h i and CEA lo cells were cultured separately, the resulting cell populations again exhibited mixed CEA expression, demonstrating that the cancer cells could switch CEA expression on and off. (genengnews.com)
First, we isolated resistant clones from a human prostate cancer cell line, PC3, after repeated exposure to Ad-REIC. (elsevierpure.com)
T cell therapy represents an emerging and promising modality for the treatment of both infectious disease and cancer. (biomedcentral.com)
Metastatic spread is responsible for the majority of cancer-associated deaths and is affected by a variety of factors, including the deregulation of gene expression, signal transduction, and epigenetic modification, as well as epithelial-to-mesenchymal transition (EMT) and the transformation of tumor cells into stem/progenitor cells. (jcancer.org)
Invading cancer cells can utilize different modes of migration including single cell migration and collective migration. (jcancer.org)
Cancer stem cells (CSCs) represent a distinct group of cells within cancerous tissue that possess the ability to initiate tumorigenesis and exhibit potency, self-renewal, and drug resistance. (bmrat.org)
2015. Human breast cancer invasion and aggression correlates with ECM stiffening and immune cell infiltration. . (ucsf.edu)
Monoclonal antibodies can modulate cancer cell signal transduction and recruit antitumor immune effector mechanisms - including antibody-dependent cellular cytotoxicity (ADCC). (researchassistantresume.com)
The SCC-61 cell line was provided TOK-001 (Galeterone) by Igor Astsaturov (Fox Chase Cancer Center FCCC). (researchassistantresume.com)
The UM-SCC-11a cell line was provided by John Deeken (Georgetown Lombardi Comprehensive Cancer Center). (researchassistantresume.com)
Stemming from these initial reports, ongoing investigations are evaluating the potential usefulness of miRNA-based therapy in cancer, and point to a potential role of sensitize cancer cells following chemo- and radiotherapy. (sarcomahelp.org)
We are using functional genomic approaches to investigate the nutrient signaling and stress adaptations of cancer cells when exposed to various nutrient deprivations and microenvironmental stress conditions. (duke.edu)
Previously, we have identified the profound cystine addiction of renal cell carcinoma (1), breast cancer cells (2, 3), and ovarian cancer cells (4). (duke.edu)
Dendritic cell (DC) vaccines are a promising and potent therapeutic tool for chronic diseases, autoimmune diseases, and cancer because of the unique ability of DCs to stimulate T cells. (bilim.edu.tr)
In her main work, she uncovered a regulatory module expressed in dendritic cells in human non-small-cell lung cancer patients, functionally validated multiple of these regulatory molecules, and showed that modulating the regulatory program in dendritic cells leads to improved tumor control. (cemm.at)
While folate is a key cofactor essential for all living cells and is a targetable vulnerability in cancer cells, our understanding of its homeostasis and usage control is rudimentary. (harvard.edu)
The presentation of brain metastases in melanoma, non-small cell lung cancer, and breast cancer and potential implications for screening brain MRIs. (moffitt.org)
Papadakis EN, Dokianakis DN, Spandidos DA: p53 codon 72 polymorphism as a risk factor in the development of breast cancer. (aktsignals.com)
Soluble factors in conditioned media from E 2 treated breast cancer cells also lead to migration and tube formation of human umbilical vein endothelial cells (HUVEC) in vitro . (biomedcentral.com)
Confounding by concomitant exposure to other cancer determinants, including arsenic, was not controlled for. (inchem.org)
Changes in these and other oncogenes can disrupt cell growth control, ultimately leading to the uncontrolled growth of cancer. (medscape.com)
The evolving culture of cancer care, puting to radiotherapy planning (e.g. nologies undeniably contribute to and cancer medicine more broadly, deep learning to facilitate automated better outcomes for cancer patients has also played a major part in these treatment contouring and planning). (who.int)
The weekly number of cancer deaths with COVID-19 as a ties and address structural and social determinants of health among cancer survivors, which might help protect those at § https://www.cdc.gov/nchs/nvss/revisions-of-the-us-standard-certificates-and- disproportionate and increased risk for death from COVID-19. (cdc.gov)
3: Mörner M, Gunnarsson U, Jestin P, Egenvall M. Volume of blood loss during surgery for colon cancer is a risk determinant for future small bowel obstruction caused by recurrence--a population-based epidemiological study. (cancercentrum.se)

There, she gained extensive knowledge of human tumor immunology and myeloid components of tumor immune suppression. (cemm.at)
To examine the relevance of these findings to the intact acidic human TME, we performed studies in fresh primary human tumor histocultures from clear cell renal cell carcinoma (ccRCC) patients with immunohistochemically confirmed high macrophage infiltration and carbonic anhydrase 9 (CA9) expression. (ox.ac.uk)

with ALV-J to induce tumors ( 1 ), fi eld all ALV isolates compared with the Eradication of ALV-J has been dif- cases of ALV-J infection and tumors HPRS-103 strain ( 5 ). (cdc.gov)
Cytotoxic T lymphocytes or helper T cells involved in the positive immune responses were easily induced by the antigen on the cell surface, whereas soluble melanoma antigens secreted into the culture medium selectively stimulate and induce antigen-specific suppressor T cells. (go.jp)

All virus iso- myeloid leukosis, detected by gross or DOI: 10.3201/eid1610.100780 lation was performed in DF-1 cells. (cdc.gov)
Myeloid derived suppressor cells (MDSC) are immune cells that dampen immune responses. (iospress.com)
What specific pathways need to be targeted in tumor-associated myeloid cells to break their immune-suppressive properties? (cemm.at)
In our group we aim to target immune-suppressive phenotypes of myeloid cells, paired with already established immunotherapies such as immune checkpoint blockade, to allow for optimal immune responses within the TME. (cemm.at)
At the mechanistic level, single cell RNA sequencing (scRNAseq) of human PBMCs obtained from healthy donors demonstrated a pronounced effect of low pH on the myeloid compartment, with a clear polarization of these cells toward an immunosuppressive character and modulation of GPR65 expression. (ox.ac.uk)

We have recently shown that an adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. (elsevierpure.com)

The oligosaccharide groups on gangliosides extend well beyond the surfaces of the cell membranes, and act as distinguishing surface markers that can serve as specific determinants in cellular recognition and cell-to-cell communication. (wikipedia.org)
Antibody-drug conjugates (ADCs) distribute cytotoxic payloads to cells with the degree and specificity of cellular uptake being crucial to the molecule's effectiveness and safety. (news-medical.net)
The research, "Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence," is published in the journal BMC Cell Biology . (sciencedaily.com)
Exhausted T cells are functionally defined by limited cytokine production and cytotoxic effector function and inefficiency to clear the cellular source of antigen. (cemm.at)
Autophagy is a cellular homeostatic mechanism where proteins and organelles are digested and recycled to provide an alternative source of building blocks and energy to cells. (nature.com)
Our biochemical and cellular analysis thus demonstrates that the GID/CTLH complex prevents cell cycle exit in G1, at least in part by degrading Hbp1. (elifesciences.org)
Cellular proteostasis involves the coordinated and compensatory action of pathways that control biogenesis, folding, trafficking and breakdown of proteins allowing the cell to adapt to physiological or pathological environmental changes. (elifesciences.org)
Understanding the cellular functions of these genes sheds light on mechanisms of normal and abnormal brain growth, as well as tumor development. (virginia.edu)

We want to develop novel immunotherapy concepts by modulating and reprogramming specific immune cell compartments to achieve optimal immunity against tumors. (cemm.at)
We are exploring how polarization/restimulation with different tumor-associated antigen-presenting cells shape T cell phenotypes and how different antigen-presenting cell subsets and phenotypes drive or limit antigen-specific anti-tumor immunity. (cemm.at)
Determinants of immunity to influenza infection in man. (cdc.gov)

We applied a multimodality preclinical imaging platform to mechanistically characterize tumor response to radiation, with a focus on HIF-1 - dependent resistance pathways. (lincoln.ac.uk)

We reported here that plakoglobin, as a key component in cell adhesion, can promote collective metastasis through facilitating IMPC clusters formation. (jcancer.org)
In comparing the clinicopathological features of 451 IMPC patients and 282 IDC-NST patients, our results showed that tumor emboli were significantly higher in IMPC patients and were associated with a high frequency of metastasis. (jcancer.org)

Finally, we succeeded in cloning of the genomic deoxyribonucleic acid (DNA) which contains genes encoding melanoma antigen with species specific determinant (M562). (go.jp)
Based on the concept that cystine deprivation triggers the ferroptosis due to the unopposed oxidative stresses, we have performed functional genomic screens to identify many novel genetic determinants of ferroptosis. (duke.edu)

To investigate CRC resistance to cibisatamab sensitivity in a relevant laboratory model the researchers generated mini-tumor PDOs from biopsy samples taken from eight CRC patients, including seven with treatment-resistant metastatic CRC, and another with treatment naïve primary CRC, and co-cultured the organoids together with the CD8 T cells. (genengnews.com)
We confirm that knockdown increases tumor cell sensitivity to ADCC while overexpression of c-Abl reduces ADCC and rescues the effects of knockdown. (researchassistantresume.com)

Morbidity rates for some fl ocks ulated intraabdominally with a 1,000- ence and Technology Development (no. reached 60%, and mortality rates for unit 50% tissue-culture infective dose 2010AA6AN034). (cdc.gov)
The following were grown in 75 cm 2 tissue culture treated flasks: BT-474 cells (DMEM+10% FCS), HT1080 cells (F12K+10% FCS, 1% glutamax, 1 u/mL pen/strep), Jurkat and Raji cells (both RPMI 1640+10% FCS). (news-medical.net)
Materials & Methods Cell lines primary cells and culture A431 A253 FaDu HNSCC 1483 SCC-4 SCC-9 and SCC-25 cell lines were obtained from the Georgetown Lombardi Tissue Culture Shared Resource (TCSR). (researchassistantresume.com)
Antibody-independent natural cytotoxicity and ADCC assays Target cells were seeded or reverse-transfected in 96-well white-walled clear bottom tissue culture plates (Corning Costar). (researchassistantresume.com)

Additionally, monoclonal antibodies are utilized within immunotherapy to flag tumor cells for immune cell clearance (e.g. (news-medical.net)

What antigen-presenting cell phenotypes play a role in T cell polarization and restimulation at the tumor site and how do they drive/limit T cell exhaustion? (cemm.at)

We used a new technique for growing miniature replicas of tumors to develop a way of testing whether patients will respond to immunotherapy," Gerlinger stated. (genengnews.com)

there is increasing data to suggest that miRNAs may act as either tumor suppressors or oncogenes. (sarcomahelp.org)

Internalization of antibodies by specific (target mediated) or non-specific (e.g. pinocytosis in endothelial cells) mechanisms forms a key determinant of the half-life in the body, and even subtle changes to the structure of monoclonal antibodies can result in considerable changes to the duration of activity. (news-medical.net)
VEGF is purified from bovine pituitary follicular cells in 1959 and is a vascular endothelial cell-specific mitogen that promotes the growth of vascular endothelial cells. (spandidos-publications.com)
Further, hypoxia and estrogen are interchangeable as both similarly modulate epithelial-endothelial cell interaction. (biomedcentral.com)

Our data suggests that using chemicals to switch back on the major class of genes that are switched off as we age might provide a means to restore function to old cells. (sciencedaily.com)
These old cells lose the ability to correctly regulate the output of their genes. (sciencedaily.com)
When activated, genes make a message that gives the instructions for the cell to behave in a certain way. (sciencedaily.com)
Most genes can make more than one message, which determines how the cell acts. (sciencedaily.com)
The CDKN2A/B locus contains genes encoding cell cycle inhibitors, including p16 Ink4a , which have not yet been implicated in the control of hepatic glucose homeostasis. (diabetesjournals.org)

Genetic ablation of ketohexokinase or stimulation of pyruvate kinase prevents villus elongation and abolishes the nutrient absorption and tumour growth that are induced by feeding mice with high-fructose corn syrup. (nature.com)
First, we are elucidating the genetic determinants and disease relevance of ferroptosis, a newly recognized form of cell death. (duke.edu)
Recently, we have found that the genetic removal of MESH1 in tumor cells triggers extensive transcriptional changes and confers protection against oxidative stress-induced ferroptosis (11). (duke.edu)
This review summarizes the major pathological factors linking obesity to diabetes, focussing on current epidemiological data related to obese diabetic patients in the Arab world, the etiology of the disease and the genetic determinants of diabetes and obesity. (who.int)

One arm of the antibody binds to carcinoembryonic antigen (CEA), a glycoprotein that is expressed on the cell surface of a wide variety of tumor types. (genengnews.com)
[ 7 ] In KAs, cells that stain positive with proliferating-cell nuclear antigen immunostaining are distributed only in the outer edges of the tumor, corresponding to the proliferating squamous epithelial cells. (medscape.com)
In contrast, cells within an SCC that stain positive with proliferating-cell nuclear antigen immunostaining are more diffusely distributed. (medscape.com)
Furthermore, the soluble antigen works as an inhibitor for the blocking of the cytotoxic T cell activity. (go.jp)
Questions that fuel our research are: How can we empower antigen-presenting cells to prime effective tumor-directed T cell responses? (cemm.at)
Unlike functional memory and effector T cells, exhausted T cells are dependent on persistence of presented antigen. (cemm.at)
Hence, direct antigen-presenting cell/T cell contact is critical in driving T cell exhaustion. (cemm.at)
Clear cell acanthoma usually stains positively for epithelial membrane antigen and negatively for carcinoembryonic antigen. (medscape.com)

Organoids largely resembled the original tumor in expression of synaptophysin, chromogranin and Ki-67. (bvsalud.org)
Initial tests with the new system first confirmed that, "as anticipated," while organoids expressing high levels of CEA (CEA hi ) were highly sensitive to treatment with CD8 T cells and cibisatamab, CEA lo PDOs that expressed only low levels of the glycoprotein were resistant to therapy. (genengnews.com)

In fact, clear cell acanthoma possesses a similar staining pattern to inflammatory dermatoses such as psoriasis vulgaris, lichen planus, and discoid lupus erythematosus and might be a localized form of inflammatory eruption rather than a neoplasm. (medscape.com)
The terms 'neoplasm' and 'tumour' are used interchangeably. (who.int)

Histologically, the fully formed domelike lesion reveals a mass of proliferating squamous epithelial cells, which created the central core of keratin. (medscape.com)
In the embryo, the cortex begins as an epithelial sheet of neural stem cells. (virginia.edu)
The stem cells must make a faithful copy of their genome, and then split in half while maintaining their polarity and attachments within the epithelial sheet. (virginia.edu)
One of the most common benign epithelial tumors is seborrheic keratosis /senile keratosis, which is a hereditary lesion that appears in persons aged more than 30 years. (medscape.com)
On the other hand, clear cell acanthoma is a rare, benign epithelial cutaneous tumor. (medscape.com)

The present study explores the antimicrobial activity and cytotoxic effects in culture assays of two fruticose soil lichens, Cladonia rangiformis Hoffm. (bilim.edu.tr)

We previously identified the acid sensing G protein coupled receptor (GPCR), GPR65, as a primary determinant of these suppressive cells. (ox.ac.uk)

Our study has found that bowel cancers have a way of dodging even the newest of immunotherapies-changing their spots by altering the levels of a key molecule on the surface of cells, so that they become harder to recognize. (genengnews.com)

TP53 status is a determinant of the functional duality of ESR2. (nih.gov)

In addition, the heterogeneous dynamics of CD8 T-cells during their interaction with lymphocytes have been investigated 13 . (nature.com)

M2590 recognized GM3 carbohydrate moiety with cross-species specificities, while M562 reacts with the protein with species specific melanoma antigenic determinant. (go.jp)
By screening for alteration in levels and functional status of proteins involved in Ad-REIC-induced apoptosis, we found that BiP/GRP78, an ER-residing chaperone protein, was expressed at higher levels consistently among resistant cells. (elsevierpure.com)
A positive TST result, consisting of measurable skin induration after the injection of tuberculin purified protein derivative, is part of a delayed-type hypersensitivity response of host immune system memory T cells sensitized by prior mycobacterial exposure [ 3 ]. (cdc.gov)

PD-L1 expression on both infiltrating immune cells and/or tumor cells can be predictive for therapeutic success of ICB, suggesting that multiple mechanisms for T cell suppression involving PD-L1 expression on different compartments exist. (cemm.at)
We are studying the mechanisms of these unusual cell divisions, and how the way that the cells divide influences the structure and fate of daughter cells. (virginia.edu)

The three main receptors used by SARS-CoV-2 to bind and gain entry into human cells are ACE, TMPRSS2, and CD147. (bmrat.org)
In recent years, the MAPK family has been found to be an important signal-regulating enzyme between cell surface receptors and determinants of gene expression ( 8 ). (spandidos-publications.com)

Typically, these technologies start by engineering a single progenitor cell with artificial transcribed recording sites that accumulate stable insertions or deletions ("indels") as a result of repair of Cas9 double-stranded breaks. (biorxiv.org)

Models derived from tumors containing both neuroendocrine and non-neuroendocrine components were at risk of overgrowth by non-neuroendocrine tumor cells. (bvsalud.org)

The location, extent and rate of internalization into cells are considered to be important antibody properties. (news-medical.net)
In either case, the rate of removal from the cell surface will impact upon the therapeutic profile of the antibody considerably. (news-medical.net)
Proper analysis involves the signal from the internalized antibody being sufficiently isolated from that outside the cells. (news-medical.net)
This white paper outlines an approach developed to address these limitations: an integrated assay solution that is based on Incucyte ® Live-Cell Analysis, along with a novel pH-sensitive dye coupled antibody fragment (Incucyte ® FabFluor Antibody Labeling Reagents). (news-medical.net)

As with all tumors, whether benign or malignant, identification of the cell or cell layer of origin is of the utmost importance. (medscape.com)
As with all tumors, whether benign or malignant, the paradigm of comprehension depends on identifying the cell or cell layer of origin. (medscape.com)

Our data indicate that plakoglobin promotes tumor cluster formation in IMPC and downregulates apoptosis in the cell clusters through activation of PI3K/Akt/Bcl-2 signaling. (jcancer.org)
One consequence of defective abscission in the embryonic neural stem cells is p53 elevation and programmed cell death (apoptosis). (virginia.edu)

Frozen primary peripheral blood mononuclear cells (PBMC) from three individual donors (AllCells) were enriched for NK cells (Human NK Cell Enrichment Kit STEMCELL Technologies) yielding 3.6-6.7% of total PBMCs maintained in RPMI-1640 with 10% FBS and 2 mM L-glutamine and stimulated with 500 units/mL recombinant human IL2 (Life Technologies). (researchassistantresume.com)

Reporter cells were serially interrogated in vitro before and after irradiation as monolayer and multicellular spheroid cultures and as subcutaneous xenografts in nu/nu mice. (lincoln.ac.uk)

KA lesions can easily be mistaken for squamous cell carcinoma (SCC). (medscape.com)
Methods: C6 glioma and HN5 human squamous carcinoma cells were stably transfected with a dual HIF-1 signaling reporter construct (dxHRE-tk/eGFP-cmvRed2XPRT). (lincoln.ac.uk)
The image below shows an early oral squamous cell carcinoma (SCC) on the midlateral border of the tongue, previously diagnosed as a lichenoid reaction to amalgam. (medscape.com)
Oral squamous cell carcinoma on the midlateral border of the tongue. (medscape.com)
Oral squamous cell carcinoma in the most common intraoral site, lateral tongue, initially reported as a chronic leukoplakia, which had become ulcerated and indurated at the time diagnosis was confirmed. (medscape.com)

J (ALV-J) infection and tumors in com- with strain-specifi c primers was used mitted from dam to progeny by the mercial layer chickens and breeders of to detect ALV-A, ALV-B, or ALV-J ( 4 ). (cdc.gov)
Many fi eld cas- tation is not found in ALV-J isolated es of ALV-J infection and tumors have from broiler chickens ( 6 ). (cdc.gov)
Infection efficiency of the adenovirus vector and expression level of REIC/Dkk-3 in the resistant clones were similar to those in the parental PC3 cells. (elsevierpure.com)
At the other end of the spectrum, patients with a recovering immune system, such as persons with HIV who are initiating antiretroviral therapy or persons stopping anti-tumor necrosis factor therapy, can have an overexuberant immune response to TB infection characterized by extensive cavitary lung lesions and necrotic lymph nodes [ 13 ]. (cdc.gov)

Anatomy25

Organisms11

Diseases72

Chemicals and Drugs50

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes52

Disciplines and Occupations1

Information Science4

Health Care7

Major determinant4

Primary and metastatic brain tumors1

Microenvironment10

Solid tumors1

Molecular17

Associated therapeutic1

Progression11

Proliferation13

Anti-tumor effects2

Cutaneous tumors5

Survival10

Colorectal cancer2

Differentiation6

Clinical6

Vivo11

Histologic2

Suppressor genes2

Effector cells5

Potential of tumor1

Fibroblasts5

Pathway5

Stromal6

Interactions8

EGFR2

Inhibition1

Effectors3

Breast tumor1

Cancer44

Human tumor2

Induce2

Myeloid5

Potent1

Cellular8

Immunity3

Pathways1

Metastasis2

Genomic2

Sensitivity2

Tissue4

Immune cell1

Phenotypes1

Immunotherapy1

Oncogenes1

Endothelial3

Genes5

Genetic4

Antigen8

Organoids2

Neoplasm2

Epithelial5

Assays1

Suppressive1

Immunotherapies1

TP531

Heterogeneous1

Protein3

Mechanisms2

Receptors2

Progenitor1

Neuroendocrine1

Antibody4

Malignant2

Apoptosis2

Peripheral blood1

Xenografts1

Squamous5

Infection4