• High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). (wikipedia.org)
  • A first method is a high-performance thin layer chromatography (HPTLC), the second is high-performance liquid chromatography (HPLC) with conductimetric detector. (edpsciences.org)
  • In this study, scientists from the USDA-ARS (Miami, FL) conducted research using high-performance thin layer chromatography (HPTLC) as a fast, reliable, non-destructive screening tool to separate TTO and other Melaleuca oil samples for identification of potential new attractants. (usda.gov)
  • oils (M. cajuputi, (MCa) two chemotypes of M. quinquenervia, (MNe and MNi) and M. ericifolia (MRo)) was carried out using gas chromatography-mass spectrometry (GC-MS) and high-performance thin layer chromatography (HPTLC). (usda.gov)
  • High-performance thin-layer chromatography (HPTLC) coupled to a genotoxicity bio-assay has been promoted as an alternative approach to assess food packaging migrates. (altex.org)
  • The SF-SBM formulation was standardized using high-performance thin-layer chromatography (HPTLC) to assess the presence of marker compounds, namely gallic acid, quercetin, and ferulic acid. (bvsalud.org)
  • ALICE core facility offers a much better solution for the characterization of low molecular weight carbohydrates and carbohydrate degradation products, namely high-performance thin layer chromatography (HPTLC), in a fully automated setup. (boku.ac.at)
  • MilliporeSigma's unique LiChrospher® HPTLC plates are the first thin layer chromatography plates based on spherical silica particles. (emdmillipore.com)
  • The spot capacity (analogous to peak capacity in HPLC) can be increased by developing the plate with two different solvents, using two-dimensional chromatography. (wikipedia.org)
  • The blood was centrifuged and the plasma stored at −25 °C until analysed by HPLC with radiochromatographic detection. (bps.ac.uk)
  • When those compounds need to be characterized, high-performance liquid chromatography (HPLC) is the traditional method of choice which however, soon comes to its limits when encountering the overly complex product mixtures typical of biorefineries. (boku.ac.at)
  • Our laboratory is home to a brand new HPLC (High Performance Liquid Chromatography) system. (herbalapothecaryuk.com)
  • 1 extraction was carried out using immunoaffinity column, separated by reversed-phase (C-18) high performance liquid chromatography (HPLC) , and quantified by fluorescence detector. (bvsalud.org)
  • Gas chromatography with flame ionization detection indicated that the main ingredient differed from the molecules of the same class already known. (erowid.org)
  • Chromatographic separation of drugs was performed over thin layered chromatography plates pre-coated with silica gel 60F254 using toluene:acetone:formic acid (3.5:5.5:1 v/v/v) as the mobile phase via a linear ascending technique in twin trough chamber. (ijpsonline.com)
  • The most widely used method for the biochemical screening of oligosaccharidoses is the analysis of the urinary oligosaccharide pattern by thin-layer chromatography on silica gel plates. (nih.gov)
  • Metabolite characterization of SF-SBM was carried out using ultra-high-performance liquid chromatography mass spectrometry (UPLC-MS) with a monolithic capillary silica-based C18 column. (bvsalud.org)
  • The method specified in ISO 8128-2:1993 includes the extraction of patulin in a mixture of ethyl acetate and chloroform (3:2 by volume), filtration of the extract on a silica-gel column, and qualitative and quantitative determination by means of two-directional thin-layer chromatography. (ansi.org)
  • The principal method used for the detection of diazinon or its metabolites in biological samples is gas chromatography (GC) using a flame photometric detector (FPD), a mass spectroscopy detector (MS), an electron capture detector (ECD), or a flame ionization detector (FID). (cdc.gov)
  • A new Amperometric detector has been added to Metrohm's chromatography systems, which improves selectivity and offers enhanced measurement sensitivity. (chromatographytoday.com)
  • The principle of the method specified in ISO 8128-1:1993 is extraction of patulin from a test portion using ethyl acetate followed by partitioning of the extract with aqueous sodium carbonate solution, qualitative and quantitative determination of the patulin content by means of high-performance liquid chromatography using an ultraviolet detector. (ansi.org)
  • High-performance liquid chromatography is the most widely used chromatographic method applied for biogenic amines in foods. (usp.br)
  • Provides elution of chromatograms on a thin layer of sorbent deposited on plates made of aluminum foil or glass (chromatographic plates with dimensions up to 200 x 200 mm). (petrolaser.com)
  • Chromatography is a chemistry separation technique used to characterize biogenic amines in foods. (usp.br)
  • This research will lead to the separation, isolation and identification of novel, alternative attractants for male medfly, with potential applications for the improvement of detection and management programs for this major agricultural pest. (usda.gov)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
  • 10‐[ 3 H]‐DHA was synthesized by reduction of artemisinin with sodium boro‐[ 3 H]‐hydride and purified with preparative thin layer chromatography. (bps.ac.uk)
  • Thin layer chromatography of methanol extracts of leaflets, petioles, crowns, and roots separated the 6-hydroxymethyl derivative, and several unidentified metabolites, including a major water-soluble metabolite at the origin. (bioone.org)
  • Detection and identification of mexacarbate and some of its metabolites from forestry substrates by thin layer chromatography.FPMI File Report 56. (gc.ca)
  • Thin-layer chromatography-directed bioautography was performed to determine the phytochemicals of the extracts and their antimicrobial activity. (ijpsonline.com)
  • The bioautography of TLC chromatogram showed single band exhibited antibacterial activity and phytocompound from this band was isolated and identified as 5,6,7,8-tetrahydro-1,2,4-benzotriazine-3-amine using gas chromatography-mass spectroscopy analyses. (ijpsonline.com)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • In this work, the analysis of the urine oligosaccharide pattern was standardized for the first time by using capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection (Beckman P/ACE MDQ) with a 488-nm argon ion laser module. (nih.gov)
  • Since it is based on the indirect fluorescence mode, universal detection is possible without derivatization. (mst.edu)
  • Thin Layer Chromatography (TLC) analysis was used to authenticate the AP extracts as judged by the detection of andrographolide (AND). (scialert.net)
  • A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements. (wikipedia.org)
  • 2001) reported a method for diazinon identification and quantification in human blood using SPE, GC-nitrogen phosphorus detection (NPD) analysis followed by GC-MS confirmation. (cdc.gov)
  • A highperformance thin layer chromatography method was developed for simultaneous estimation of mangiferin and berberine from setebid tablets. (ijpsonline.com)
  • The method was validated according to the international conference on harmonisation Q2 R1 guidelines for accuracy, precision, limit of detection, linearity, limit of quantification, robustness and specificity. (ijpsonline.com)
  • A laser-based indirect fluorometric detection method for thin-layer chromatography is described. (mst.edu)
  • A sensitive liquid chromatography - electro spray ionization mass spectrometry (LC-ESI-MS) method is developed and validated for rapid determination of Esomeprazole in human plasma. (ajrconline.org)
  • The method is highly selective, has good repeatability and has limits of detection and quantification in the nanogram range. (boku.ac.at)
  • the second part, ISO 8128-2:1993 specifies a method using thin layer chromatography. (ansi.org)
  • ISO 8128-1:1993-Apple Juice, Apple Juice Concentrates And Drinks Containing Apple Juice - Determination Of Patulin Content - Part 1: Method Using High-Performance Liquid Chromatography is available on the ANSI Webstore. (ansi.org)
  • Detection and quantification were carried out at an iso-absorptive wavelength of 261 nm using thin layered chromatography scanner III. (ijpsonline.com)
  • Laser using -Based Indirect Fluorometric Detection and Quantification " by Yinfa Ma, Lance B. Koutny et al. (mst.edu)
  • Laser using -Based Indirect Fluorometric Detection and Quantification in Thin-Layer Chromatography," Analytical Chemistry , vol. 61, no. 17, pp. 1931 - 1933, American Chemical Society (ACS), Sep 1989. (mst.edu)
  • These two methods are rapid and particularly the thin-layer chromatography. (edpsciences.org)
  • Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits and/or to improve accuracy and precision. (cdc.gov)
  • Such inactivated reference materials were used as positive controls in assays to detect suspected B. anthracis in samples because live agent cannot be shipped for use in field settings, in improvement of currently deployed detection methods or development of new methods, or for quality assurance and training activities. (cdc.gov)
  • Current methods used to detect the compound involve two separate steps - an immunoassay followed by detection using techniques such as thin-layer chromatography or mass spectrometry. (chemistryworld.com)
  • The gas chromatography-mass spectroscopy analysis was performed for the isolated active components exhibited antimicrobial activity. (ijpsonline.com)
  • In order to work out the diagnostic features, the formulation was studied on the basis of pharmacopoeial parameters such as microscopy, physico-chemical analysis, thin layer chromatography, level of microbial and pesticide contaminations, aflatoxins and the heavy metals. (ajrconline.org)
  • This results in shorter analysis times and improved detection limits. (emdmillipore.com)
  • The Applied Biosystems'™ Axiom™ Microbiome Array (ABAMA) is the commercialized version of LLNL's microarray innovation, the Lawrence Livermore Microbial Detection Array (LLMDA). (llnl.gov)
  • Early detection of a biological threat is critical not only for identifying the threat organism but also for implementing appropriate countermeasures to save and protect the victims and prevent further infection and for decontaminating and reclamating the affected environment and infrastructures. (cdc.gov)
  • 2003). Biologische Detektionsverfahren in der Dünnschichtchromatographie (Biological detection procedures using thin-layer chromatography). (altex.org)
  • Concentration and 1987 greater purification by sweep co-distillation omentum) and Florisil/anhydrous sodium sulfate column chromatography. (cdc.gov)
  • 1982). Detection of mutagens in complex samples by the Salmonella assay applied directly on thin-layer chromatography plates. (altex.org)
  • While there are various forms of presumptive drug testing, two of the most common techniques are immunoassay testing and thin-layer chromatography. (boreal.com)
  • In this activity, students will perform a simulated immunoassay test on several urine samples for the detection of the cocaine metabolite benzoylecgonine. (boreal.com)
  • Using the Svennerholm system of abbrevations, gangliosides are designated G for ganglioside, plus subscript M, D, or T for mono-, di-, or trisialo, respectively, the subscript letter being followed by a subscript arabic numeral to indicated sequence of migration in thin-layer chromatograms. (lookformedical.com)
  • After 30 min, the reaction was quenched in 500 mL H2O, the top hexane layer separated, and the aqueous phase extracted with 2x75 mL of petroleum ether. (erowid.org)
  • however, the food matrix complex requires that changes in the methodology of extraction, derivatization and detection must be performed according to each group of foods. (usp.br)
  • The limits of detection were 26.18 and 18.50 ng/spot for mangiferin and berberine, respectively. (ijpsonline.com)
  • Determination of hemicelluloses and pectins in wood and pulp fibres by acid methanolysis and gas chromatography. (boku.ac.at)
  • Investigations for the detection of genotoxic substances on TLC plates. (altex.org)
  • The prostatic secretion, influenced by dihydrotestosterone, is a whitish (sometimes clear), thin fluid containing proteolytic enzymes, citric acid, acid phosphatase and lipids. (forensicsdigest.com)
  • Extraction and detection of nicotine and caffeine. (pte.hu)
  • The microarray is the most comprehensive microorganism detection platform built to date and the first high throughput microarray that employs whole genome resolution for identifying all sequenced microbes. (llnl.gov)
  • To ensure a correct sampling, each bag of 25 kg had a linear imaginary division in its length into three equal parts from which primary samples (1 kg) from the upper layer, central layer, and lower layer were collected. (thefishsite.com)
  • With the 96-well ABAMA, the new detection system can analyze 96 samples in three days. (llnl.gov)
  • The amperometric detection is described by the company as far more "robust" and easier to use than ever before, exhibiting strikingly low noise and excellent signal strength as a consequence of the three electrode design in the wall-jet or thin-layer geometry. (chromatographytoday.com)
  • The limit of detection (LOD) and the limit of quantitation (LOQ) reported in the study are 1.97 and 6.58 g/L, respectively. (cdc.gov)
  • Scientists from Spain have used a lab on a chip device to quickly analyse levels of zearalenone, a contaminant, in infant foods with a detection limit below legislative requirements. (chemistryworld.com)
  • Based on binding potential measured by PET, the limit of detection was about 160,000 CD25-positive cells per 200 μl lesion (95 % confidence). (springer.com)
  • Also, it takes only 35 s for acquiring a data array of 256 × 64 points with this technique to achieve a detection limit of 6 pg. (mst.edu)
  • The limit of detection is 10 µg/l, based on 5 ml of ready-to-drink apple juice. (ansi.org)
  • The limit of detection is 25 µg/l, ba. (ansi.org)
  • Detection of drugs in sweat and hair is a recent addition to drug abuse detection technology. (medscape.com)