Detection compounds drug screening assays antitumor. Medical search. Frequent questions

Various natural compounds with promising in vitro and in vivo anti-inflammatory activities have been reported in literature which can be used as novel therapeutic approach for treatment of inflammatory conditions [ 7 ]. (biomedcentral.com)
A recent in vitro screening of the Phoneutria nigriventer spider venom (PnV) antitumor effects by our group has shown that the venom significantly affected glioblastoma cell lines. (nature.com)
Examples were given of different drug discovery programs developed at MEDINA from its microbial Natural products libraries: discovery of a novel family of antibiotic natural products with Gram-negative broad spectrum, search for antitumor compounds in the nuclear translocation-based assay, Identification of a novel Inhibitor of VHL/HIF Pathway and detection of a novel molecule with protective effects on neurodegenerative in vitro models. (medinadiscovery.com)
Furthermore, it is highly versatile and suitable for applications in the preclinical drug research and in vitro assays. (bvsalud.org)
Prediction of Chemical Carcinogenicity in Rodents from in Vitro Genetic Toxicity Assays R AYMOND W. TENNANT, BARRY H. MARGOLIN, MICHAEL D. SHELBY, E RROL Z … As an in vivo toxicity endpoint, the acute lung inflammogenicity in a rat instillation model was compared with the in vitro toxicity endpoints comprising cytotoxicity, pro-inflammatory cytokine expression, or haemolytic potential. (holocausto.online)
In vitro toxicity assays are used to determine the potential of a new pharmaceutical, agrochemical, food additive, or other chemical product to be hazardous to humans.In vitro studies, which are performed on cultured bacterial or mammalian cells, can be used as an initial screen to avoid the unnecessary use of animals in determining which candidates should move forward for further safety testing. (holocausto.online)
The application of High Content Screening for in vitro toxicity testing is a relatively new approach in the preclinical research phase of drug development. (holocausto.online)
In regulatory testing situation these in vitro toxicity assays must be reliable, because from these tests it is only possible to generalize the data to … First Published 1995. (holocausto.online)
Kroll A(1), Pillukat MH, Hahn D, Schnekenburger J. The in vitro assays covered baseline toxicity in bacteria, oxidative stress, cytotoxicity in mammalian cells and yeast, estrogenicity, and anti-androgenicity. (holocausto.online)
While toxicity test has been traditionally completed in the preclinical phase, in vitro toxicity studies in the early drug discovery stages could significantly reduce failures at a later stage and prevent economic loss. (holocausto.online)
Considering the ethical issues and the cost of in-vivo animal tests, the pharmaceutical industry now relies more on in-vitro methods for toxicity testing in the drug development phase. (holocausto.online)
Traditionally, the in vitro toxicity assays that have been used to identify molecules that carry drug-related DILI risk factors include two-dimensional (2D) cell systems and other standard preclinical model… The principal application of this assay is to rapidly screen test compounds to identify those that induce acute liver cell toxicity. (holocausto.online)
In vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc. (holocausto.online)
H … In vitro assays covered baseline toxicity, oxidative stress responses, and estrogenic and antiandrogenic activity. (holocausto.online)
The positive control induced a high degree of toxicity in all in vitro tests performed, indicating that the toxicity observed in the direct contact assay was due to in situ extraction of toxic components. (holocausto.online)
Albumin binding affinity test and in vitro control-released assay were performed to screen these BF-30 derivatives and explore the mechanism of anti-tumour effects. (jcancer.org)
Surface plasmon resonance (SPR) measurements, plasma stability test and in vitro control-released assay all showed that LBF-14 exerted better druggability compared with the others. (jcancer.org)
By enabling cross-programmatic early translational efforts, we can leverage novel findings to identify and validate new targets for cancer, develop small molecule compounds to modulate identified targets, and test potential drug candidates in vitro and in state-of-the-art animal models of cancer. (sbpdiscovery.org)
2000. Results of the L5178Y mouse lymphoma assay and the Balb/3T3 cell in vitro transformation assay for eight phthalate esters. (cdc.gov)

The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. (biomedcentral.com)
During Brine Shrimp Lethality Assay, all extracts of BG were found to be bioactive and the degree of lethality was found to be concentration dependent. (biomedcentral.com)
The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plant-derived anti-tumor compounds. (biomedcentral.com)
The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)

Bioactive compounds produced by fungal endophytes have potential benefits, such as low cost, rapid growth, facile genetic manipulation, and industrial scale-up with fewer negative effects. (degruyter.com)

Phenolic compounds, which are secondary plant metabolites, are considered an integral part of the human diet. (mdpi.com)
however, these ingredients are phenolic compounds. (spandidos-publications.com)
The present study aimed to investigate components other than phenolic compounds that may have antitumor effects against colorectal cancer (CRC). (spandidos-publications.com)

Therefore, a rationale for treating hypertension would be to administer drugs or natural compounds which selectively inhibit ACE. (scielo.br)

The ability to fully evaluate the correlation and sensitivity of CLI to PET is critical to progress this technique further for use in high-throughput screening of pharmaceutical compounds. (snmjournals.org)
Future high-throughput studies should provide for quicker turnaround and higher cost-to-return benefits in the drug discovery process. (snmjournals.org)
A small-molecule library enriched for FDA-approved drugs was screened with a high-throughput, ligation-mediated amplification assay with a fluorescent, bead-based detection. (broadinstitute.org)
Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)
Recent advances in high-throughput and highly sensitive detection methods have overcome the limitation of the low concentration of most peripheral blood biomarkers. (techscience.com)
This program brings together the complementary strengths and resources of its members and provides expertise in assay development, high throughput screening, downstream medicinal chemistry and pharmacology through its partnership with the Prebys Center. (sbpdiscovery.org)
In this case, phenotypic screening relies on an appropriate tissueor cell-based assay that can be miniaturized and used in combination with high throughput tools. (docksci.com)

Ultimately, the targeted alkaloid detection list was built as premise relying on alkaloid cleavage pathway, and a total 87 compounds were identified. (bvsalud.org)
We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
Both diagnostic assays to test for defects in this DNA repair pathway and HER2 inhibitors are FDA-approved in cancer patients and can be easily used in the breast cancer context. (sbpdiscovery.org)

Studies have found very good relationship between this simple, inexpensive, and bench-top assay and the antitumor potential of the cytotoxic compounds [ 10 ]. (biomedcentral.com)
Despite the identification of tumor-specific somatic mutations, most cancer therapy still utilizes nonspecific, cytotoxic drugs. (broadinstitute.org)
The MTT assay was used to examine the cytotoxic effects of fungal taxol against the human liver cancer cell line HepG2. (degruyter.com)

Signature-based small molecule screening identifies cytosine arabinoside as an EWS/FLI modulator in Ewing sarcoma. (broadinstitute.org)
Unfortunately, many classes of oncoproteins (e.g., transcription factors) are not amenable to conventional small-molecule screening. (broadinstitute.org)
Previously, this target has been intractable to modulation with traditional small-molecule library screening approaches. (broadinstitute.org)
We hypothesize that screening small-molecule libraries highly enriched for FDA-approved drugs will provide a more rapid path to clinical application. (broadinstitute.org)
We demonstrate that a gene expression-based approach to small-molecule library screening can identify, for rapid clinical testing, candidate drugs that modulate previously intractable targets. (broadinstitute.org)
Amgen, in collaboration with Carmot therapeutics, was developing a Cys-12 covalent small molecule, and their initial compound showed multi-fold enhancement in potency compared to ARS-1620 in part through binding a cryptic pocket comprised of Y96/H95/Q99 in KRASG12C (6) . (cytoskeleton.com)

To achieve this milestone, it must first be established that CLI data correlate to PET data in an in vivo preclinical antitumor study. (snmjournals.org)
To facilitate the translation of imaging biomarkers from research to development, many preclinical imaging facilities use a multimodal approach, wherein the strengths of each individual modality can be combined and leveraged for the development of robust assays ( 13 , 14 ). (snmjournals.org)

Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
We conduct cytotoxicity estimation using a panel of cell proliferation and viability tests, which can be sorted into four types by different endpoints: Functional assays: assessment of ATP, ADP, ATP/ADP ratios. (holocausto.online)
Using a tetrazolium-based assay, the dose required to reduce cell viability by 50% (ED 50 ) was found to be ∼1.8 μmol/L in FaDu (human hypopharyngeal squamous cancer) and ∼2.6 μmol/L in C666-1 (human undifferentiated nasopharyngeal cancer) cells. (aacrjournals.org)
FDA-approved drugs were screened for their ability to sensitize TRAIL resistant prostate cancer cells to TRAIL using an MTT assay for cell viability. (biomedcentral.com)
Cell proliferation assays demonstrated that treatment with the more than 10,000 molecular weight fraction significantly inhibited viability in DLD‑1 cells. (spandidos-publications.com)

Molecules from animal venoms are promising candidates for the development of new drugs. (nature.com)
Studies in progress are selecting the venom molecules with antitumor and immunomodulatory effects and trying to better understand their mechanisms. (nature.com)
The identification, optimization and synthesis of antineoplastic drugs from PnV molecules may lead to a new multitarget chemotherapy. (nature.com)
Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)
Organic compounds are molecules that contain carbon bonded to another element such as hydrogen. (peroxidasessignaling.com)
A large number of potential drug targets have been cloned and functionally expressed, and enormous progress has been made in the development, miniaturization and automation of cell based assays on target molecules recombinantly expressed in mammalian cell lines. (docksci.com)

In previously published work, we demonstrated the first in vivo use of Cerenkov luminescence imaging (CLI) with a highly sensitive and ultracooled charged-coupled device camera, allowing detection of this optical emission ( 15 ). (snmjournals.org)
The drug underwent significant rounds of modifications to produce a drug suitable for in vivo applications, these efforts resulted in ARS-1620 which has been used by the scientific community to further define the utility of this class of drugs (5) . (cytoskeleton.com)
Screening in vivo may be feasible, if a good starting point for Medicinal Chemistry and a clear strategy for differentiation exist. (docksci.com)
2012). However, many drug discovery efforts rely on screening to identify starting points for Medicinal Chemistry, and screening in vivo in most cases is not commercially or ethically viable. (docksci.com)

These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)

To the best of our knowledge, no reports are available for the screening of endophytic fungal taxol in this medicinal plant. (degruyter.com)
In the future, Cochliobolus hawaiiensis could be a new source of taxol, a revolutionary therapeutic drug. (degruyter.com)

The search for new groups of anti-inflammatory and analgesic drugs is a topical issue of the current medicinal chemistry. (edu.ua)

Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. (lookformedical.com)
1983. A radio tracer study of air-water exchange of synthetic organic compounds. (cdc.gov)
Hierarchical analytical approach to evaluating the transport and biogeochemical fate of organic compounds in sewage-contaminated groundwater, Cape Cod, MA. (cdc.gov)
The susceptibility of but a few known transplacental car and reach the fetus, a carcinogenic the fetus relative to that of adult rats cinogens are organic compounds chemical may have no transplacen is measured as the incidence and that act principal y or entirely by a ge tal carcinogenic activity or may only multiplicity of tumours that develop notoxic mode of action. (who.int)

As a general category, biomarkers comprise peripheral, tissue biopsy, and imaging methods and allow for a more refined clinical development path forward for therapeutic compounds ( 1 , 2 ). (snmjournals.org)
Hepatotoxicity (toxic effects on the liver) and cardiotoxicity (toxic effects on the heart) are the most severe problems encountered in drug development and the major reason for drug withdrawals. (holocausto.online)
M. Gaba, C. Mohan, Development of drugs based on imidazole and benzimidazole bioactive heterocycles: recent advances and future directions. (dntb.gov.ua)
Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
Additional screens for MMTV provirus entry sites in tumors revealed several other upregulated genes that are associated with other gene development pathways such as INT2, INT3, and INT4 [4,5,6]. (chemdiv.com)
Therefore, AGEs in MSpf may be potential compounds for the development of antitumor drugs for the treatment of CRC with fewer adverse effects compared with existing antitumor drugs. (spandidos-publications.com)
Viswanathan and colleagues reviewed the recent progress in the culture of hPSCs with emphasis on the importance of the environment surrounding these cells and high content analysis approaches for assay development. (docksci.com)
Radiotheranostics, injectable radiopharmaceuticals with antitumour effects, have seen rapid development over the past decade. (harvard.edu)

O. Nagaraja, Y.D. Bodke, I. Pushpavathi, S. Ravi Kumar, Synthesis, characterization and biological investigations of potentially bioactive heterocyclic compounds containing 4-hydroxy coumarin. (dntb.gov.ua)
A series of compounds containing heterocyclic cores and gem -difluorocycloalkyl substituents was obtained under conditions of the parallel synthesis (i.e., simultaneous performance of reaction procedures, treatment of the reaction mixture, and product isolation for a number of similar transformations) using the reductive amination - the "Nitrogen deletion" reaction sequence. (edu.ua)
The synthesis of the target compounds commenced from heteroaromatic aldehydes and the corresponding gem -difluorocycloalkyl or ( gem -difluorocycloalkyl)methyl amines and included the NaBH 3 CN-mediated reductive amination and "Nitrogen deletion" upon the action of Levin's anomeric amide. (edu.ua)
Synthesis of new cyclitol compounds that influence the activity of phosphatidylinositol 4-kinase isoform, PI4K230. (embl.de)

Mammalian genotoxicity assay reveals if the compound causes abnormalities in chromosome distribution (aneugenity) or chromosome breaks (clastogenity) during cell division. (holocausto.online)

Pharmacophore-based activity profiling screen demonstrated some biological targets that MS44-53 may modulate their biological response, and thus can be considered as potential drugs to treat different kinds of diseases, such as carcinoma, diabetes type II, bacterial infection and cardiovascular diseases. (openmedicinalchemistryjournal.com)

The 25 representative differential metabolites were screened out successfully and divided into three categories to differentiate TFD, TFB, and TCW. (bvsalud.org)

Proliferation assays However, in rats just minimal alterations were noted. (holocausto.online)
Promising examples include anti-microbial assays, cell proliferation, platelet aggregation, and insulin release from pancreatic β-cells. (docksci.com)

The drug can be obtained from yew ( Taxus sp. (degruyter.com)

Tubulin Polymerization Assay: 99% pure porcine tubulin, fluorescence based (Cat. (cytoskeleton.com)
Sequential and simultaneous dosing modalities were investigated and the annexin V/propidium iodide assay, in concert with fluorescence microscopy, was employed to visualize cells undergoing apoptosis. (biomedcentral.com)

Endophytes still present themselves as a peculiar source of possible drugs. (frontiersin.org)

Here, we report the draft genomes for 45 Enterobacterales clinical isolates, including historical and contemporary drug-resistant organisms, obtained in Pakistan between 1998 and 2016: 5 Serratia, 3 Salmonella, 3 Enterobacter, and 34 Klebsiella. (cdc.gov)
These fundamental disadvantages often cause LDL spillover into EV isolates, thus confounding assay results. (harvard.edu)

Cytology/Membrane leakage assays: LDH, AK test. (holocausto.online)

Only a limited number of compounds are available for clinical use despite the fact that a very large variety of antiandrogen compounds have been discovered and researched. (wikipedia.org)
Recently, a drug that targets mutant RAS G12C specifically has shown promising clinical results and is now the first FDA approved RAS-targeting drug for the treatment of NSCLC, read on for a summary of this drugs journey from discovery to approval, and roadblocks that still lie ahead. (cytoskeleton.com)
The ToGA trial must be characterized as a landmark in the treatment of gastric cancer and it has paved the way for a number of new HER2 targeted compounds such as pertuzumab, ado-trastuzumab emtansine, lapatinib, afatinib, and dacomitinib, which are currently undergoing phase II and III clinical testing. (wjgnet.com)
Drugs which activate this p53 and another class of drug called BET inhibitors both show encouraging pre-clinical activity, but limited clinical activity as single agents. (sbpdiscovery.org)
This approach has delivered many clinical candidates but relatively few new drugs. (docksci.com)

Alternative drugs have been developed to target Farnesyl transferase and downstream MEK targets, but these have failed for various reasons (reviewed in (1, 2)) . (cytoskeleton.com)
Unfortunately, some targets with potent oncogenic activity fall into this category, and currently little is known about how to solve this problem, which largely hampered drug research on human cancers. (techscience.com)
Most of these drugs interact relatively weakly with a number of targets, and the complete profile of their molecular interactions is not well-known. (docksci.com)

This heterocyclic system is structurally close to 2H-1,2-benzothiazin-4-one 1,1-dioxide, which is the core of the famous non-steroidal anti-inflammatory drugs related to the "oxicam" group. (edu.ua)

Here we describe a gene expression-based approach to identify compounds that induce a signature of EWS/FLI attenuation. (broadinstitute.org)

Abstract Circulating tumor cells (CTCs) are essential biomarkers for liquid biopsies, which are important in the early screening, prognosis, and real-time monitoring of cancer. (techscience.com)

The identification of a number of FDA-approved drugs as TRAIL sensitizers can expand chemotherapeutic options for combination treatments in prostate and pancreatic cancer diseases. (biomedcentral.com)

Previous researches revealed that BF-30 is a phenylalanine- and lysine-rich polypeptide exhibiting broad spectrum and strong antibacterial properties without drug resistance. (jcancer.org)

Screening of chemical libraries for AR blockers led to the discovery of the first antiandrogen, cyproterone. (wikipedia.org)
The limited throughput of phenotypic assays compared to most target-based assays necessitates smaller libraries that are optimized with regard to biological and chemical diversity. (docksci.com)
Wassermann and colleagues discuss strategies for building appropriate, well-annotated compound libraries. (docksci.com)

iii) screen hIDO1 (human indoleamine 2,3-dioxygenase) inhibitors and evaluate their effect on downstream TRP-catabolizing enzymes. (bvsalud.org)

Since in classic chemotherapies it is observed a decrease in immune response, methotrexate (MTX) was used only to compare the PnV effects on innate immune cells with a highly immunosuppressive antineoplastic drug. (nature.com)
However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)

This assay has been extensively used for different studies such as for preliminary toxicity screening of plant extracts, detection of fungal toxins, plant extract toxicity, heavy metals, cyanobacteria toxins, pesticides, and cytotoxicity testing of dental materials [ 9 ]. (biomedcentral.com)
Phytochemical screening test revealed the presence of terpenoid, flavonoid, tannin, saponin and phenolic compound in all the stem bark extracts of C. odontophyllum. (scirp.org)

Target based screening is likely to provide very good drug candidates for monogenic diseases, and the following collection of manuscripts is not meant to discourage the use of target based approaches. (docksci.com)
Here, target agnostic approaches using phenotypic assays may offer significant benefit. (docksci.com)

DNA content analyses and Hoechst 33342 staining revealed that this compound induced apoptosis. (aacrjournals.org)
In the current work, we screened a small library of fifty-five FDA and foreign-approved anti-neoplastic drugs in order to identify candidates that sensitized resistant prostate and pancreatic cancer cells to TRAIL-induced apoptosis. (biomedcentral.com)

The GC-MS analysis of the methanolic extract of BG revealed 23 compounds which are reported to possess different biological activities. (biomedcentral.com)
Various anti-oxidants have found to possess properties such as anti-atherosclerotic, antitumor, anti-mutagenic, anti-carcinogenic to name a few selected ones [ 4 ]. (biomedcentral.com)

However, several hurdles need to be overcome before widespread implementation of hPSC-based assays. (docksci.com)

This study aimed to identify the compounds that target Src to suppress lung cancer tumourigenesis and metastasis and investigate their underlying molecular mechanisms. (oncotarget.com)
Using a molecular docking approach and the National Cancer Institute (NCI) compound dataset, eight candidate compounds were selected, and we evaluated their efficacy. (oncotarget.com)
Since the advent of molecular cloning, target based screening has become the norm in pharmaceutical drug discovery. (docksci.com)
The perspective by Dr. Swinney analyzes the phenotypic end points used in the discovery of new molecular entities that have resulted from phenotypic drug discovery efforts. (docksci.com)

This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)

Aiming to clarify the differential compounds among three species of RRTF (TFD, TFB, and TCW), targeted and untargeted acquisition strategies based on high resolution mass spectrometry were established. (bvsalud.org)
Plant metabolomics analysis and multivariate statistical analysis were accomplished to screen out differential markers which were answerable for categorizing different species of RRTF. (bvsalud.org)
Smartly engineered nanocapsules can not only shrink and decompose into small-sized nanodrugs upon drug release but also can regulate the TME to overproduce reactive oxygen species for enhanced synergistic therapy in tumors. (nature.com)

However, developing SDDSs with early diagnostic capability, enhanced drug delivery and efficient biodegradability still remains a scientific challenge. (nature.com)

Zhu K, Aykas DP and Rodriguez-Saona LE: Pattern recognition approach for the screening of potential adulteration of traditional and bourbon barrel-aged maple syrups by spectral fingerprinting and classical methods. (spandidos-publications.com)

The activity of this enzyme is commonly assayed using a leucine arylamide chromogenic substrate such as leucyl beta-naphthylamide. (lookformedical.com)
Cyclophosphamide (CTX) is a commonly used alkylating anti-tumor drug. (peroxidasessignaling.com)

We obtained protein components from maple syrup by ammonium sulfate precipitation, and treatment with the protein fraction of maple syrup (MSpf) was found to exhibit a potential antitumor effect. (spandidos-publications.com)
discusses the potential for using zebrafish as a model system, enabling large scale behavior based screens for central nervous system disorders. (docksci.com)

Tulp, M. and Bohlin, L. (2002) Functional versus Chemical Diversity: Is Biodiversity Important for Drug Discovery? (scirp.org)

Researchers anywhere can explore more than 6,000 drugs in the hub and search for possible new uses for them to jump-start new drug discovery. (broadinstitute.org)

A physico-chemical screening test for chemical carcinogens: The ke test. (cdc.gov)

Imaging of 18 F-FDG uptake was performed at 5 time points after drug treatment in the subcutaneously implanted diffuse large B-cell lymphoma tumor line OCI-Ly10. (snmjournals.org)
In 1989 the United States Food and Drug Administration (FDA) approved it for use the treatment of prostate cancer. (wikipedia.org)
Smart drug delivery systems (SDDSs) for cancer treatment are of considerable interest in the field of theranostics. (nature.com)
Current tests able to detect and quantify tetrahydrocannabinol (THC), the psychoactive ingredient of marijuana (pictured), in bodily fluids are routinely used for drug use prevention and treatment. (harvard.edu)
It is caused by numerous diseases that are accompanied by pain and inflammation, as well as by imperfection of the existing drugs aimed to provide treatment of these pathological conditions. (edu.ua)

NSAIDS are widely used for their anti-inflammatory, analgesic and antipyretic activity and are among the most widely used drugs worldwide [ 6 ]. (biomedcentral.com)
Based on the significant antibacterial activity, the sorting of bacterial cells against the compounds (MID6 and MID9) was analyzed by flow cytometry. (degruyter.com)
An MTT assay was used to determine cell metabolic activity and a TUNEL assay for detecting DNA fragmentation. (holocausto.online)

We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
Norwati, D., Harmy, M.Y., Mohd Noor, N. and Amry, A.R. (2014) Colorectal Cancer Screening Practices of Primary Care Providers: Results of a National Survey in Malaysia. (scirp.org)
Potent cell growth inhibitory properties were shown by ligands MS47 and MS49 against human melanoma MDA-MB-435, colon cancer HCT-116 and COLO 205, and pancreatic cancer MIA PaCa-2 cell lines, as evidenced by MTT assay. (openmedicinalchemistryjournal.com)
This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
Drugs demonstrating the highest synergy were selected as leads and tested in different prostate and pancreatic cancer cell lines, and one immortalized human pancreatic epithelial cell line. (biomedcentral.com)
Fourteen drugs were identified as having synergy with TRAIL, including those whose TRAIL sensitization activities were previously unknown in either prostate or pancreatic cancer cells or both. (biomedcentral.com)
The program focuses on the early stages of the drug discovery process, from target validation through "proof-of concept" studies, which aims to demonstrate that a newly discovered drug candidate can successfully alter the course of cancer disease progression. (sbpdiscovery.org)

In oncology, mouse xenograft models, based on a patient's tumor cells, have been used to select between drugs (Wu et al. (docksci.com)

issues that often frustrate the present target based drug discovery programs. (docksci.com)

Anatomy14

Organisms17

Diseases24

Chemicals and Drugs91

Analytical, Diagnostic and Therapeutic Techniques and Equipment77

Psychiatry and Psychology1

Phenomena and Processes37

Disciplines and Occupations6

Information Science4

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Vitro19

Brine Shrimp Lethality Assay4

Bioactive compounds1

Phenolic compounds3

Natural compounds1

High-throughput7

Pathway3

Cytotoxic3

Small-molecule6

Preclinical2

Viability5

Molecules6

Vivo4

Biologics1

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Development9

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Mammalian1

Modulate1

Metabolites1

Proliferation2

Taxus1

Fluorescence2

Endophytes1

Isolates2

Membrane1

Clinical5

Targets3

Heterocyclic1

Induce1

Abstract1

Identification1

Broad spectrum1

Libraries3

Downstream1

Effects2

Extracts2

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Possess2

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Molecular4

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Species3

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