• In the "simple-diploid" cases no or few genetic alterations could be detected, whereas the "complex-diploid" samples numerous aberrations (equal or more than 3) could be found. (biomedcentral.com)
  • In three samples with different clonal chromosome abnormalities, aCGH and cfDSA detected comparable copy number aberrations and further defined the chromosomal abnormalities. (najms.com)
  • In one case with FISH-detected deletions of 7q and 20q in 11-12% of cells, neither cfDSA nor aCGH detected any copy number aberrations. (najms.com)
  • A greater number of aberrations were detected in CIN+ than MSI+ samples as well as differences in the type and extent of change reported. (ox.ac.uk)
  • This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resolution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized. (wikipedia.org)
  • This is achieved through the use of competitive fluorescence in situ hybridization. (wikipedia.org)
  • To evaluate the technical and clinical feasibility of next-generation sequencing on cfDNA for detecting leukemic clonal abnormalities, a pilot study was performed on ten residual samples to compare results from cfDNA sequencing analysis (cfDSA) with diagnostic findings from karyotyping, fluorescence in situ hybridization (FISH) and array comparative genomic hybridization (aCGH). (najms.com)
  • Comparative genomic hybridization (CGH) is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. (wikipedia.org)
  • Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. (idtdna.com)
  • Standard chromosome analysis by G-banding has a limited resolution, but molecular cytogenetic techniques, such as multi-subtelomeric FISH, microdeletion FISH, multicolour FISH and comparative genomic hybridisation (CGH), have played an important role for the diagnosis of MR during the past decade. (bmj.com)
  • Comparative genomic hybridization (CGH) has been developed to elucidate genome-wide sequence copy number variation (CNV) between different genomes, such as the differential amplification or deletion of genetic regions between tumor DNA and normal DNA from neighboring unaffected tissue [ 7-10 ]. (idtdna.com)
  • Array comparative genomic hybridization, with a genome-wide resolution of approximately 1 Mb, has been used to investigate copy number changes in 48 colorectal cancer (CRC) cell lines and 37 primary CRCs. (ox.ac.uk)
  • In this study, we undertook extensive genome-wide comparative analyses of twelve species that conform the Pectobacterium genus. (mdpi.com)
  • We analyzed genetic changes in condylomas (four cases), vulvar intraepithelial neoplasia I-III (VIN I-III, eleven cases), and primary vulvar squamous cell carcinomas (VSCC, ten cases) by high-resolution comparative genomic hybridization (HR-CGH) and flowcytometry. (nih.gov)
  • Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research. (idtdna.com)
  • Many genetic disorders can be detected early in pregnancy using various noninvasive and invasive techniques. (medscape.com)
  • Aneuploid tumors contained large numbers of genetic alterations with the sum gain of at least 2 chromosomes (A-, B- or C-group) detected by HR-CGH. (biomedcentral.com)
  • Scholars@Duke publication: Comparative genetic patterns of glioblastoma multiforme: potential diagnostic tool for tumor classification. (duke.edu)
  • Although this profile is potentially useful in distinguishing GBM from other tumor types, the techniques used tend to be labor intensive, and some can detect only gains or losses of genetic loci. (duke.edu)
  • These findings should be verified using techniques that can detect smaller genetic changes, such as microarray-CGH. (bmj.com)
  • Genetic studies have clarified that most microcephaly genes encode ubiquitous proteins involved in mitosis and in maintenance of genomic stability, but the effects of their inactivation are particularly strong in neural progenitors. (cancerindex.org)
  • The aim of this technique is to quickly and efficiently compare two genomic DNA samples arising from two sources, which are most often closely related, because it is suspected that they contain differences in terms of either gains or losses of either whole chromosomes or subchromosomal regions (a portion of a whole chromosome). (wikipedia.org)
  • Routine chromosome analysis (450-500 bands) had failed to detect the rearrangements in all cases. (bmj.com)
  • In patients with hematological malignancies, the cancerous cells undergoing apoptosis could release leukemic cfDNA into the blood plasma or bone marrow fluid, and the chromosomal profiling from those cfDNA could be used to detect clonal chromosome abnormalities. (najms.com)
  • The result from this pilot study demonstrated that leukemic cfDNA in the blood plasma, and possibly bone marrow fluid, could be used to detect clonal chromosome abnormalities. (najms.com)
  • Comparative genomic hybridisation (CGH) was used to screen 76 vestibular schwannomas from 76 patients (66 sporadic and 10 NF2 related) to identify other chromosome regions that may harbour genes involved in the tumorigenesis. (bmj.com)
  • Development of comparative physical gene maps for cattle, sheep, goat and deer by fluorescence in situ hybridisation (FISH) using ovine bacterial artificial chromosome (BAC) clones. (edu.au)
  • In both cases, DNA from two samples are differentially labeled with fluorescent dyes and co-hybridized to immobilized genomic capture probes. (biomedcentral.com)
  • Cases 9 and 10 were detected using microdeletion FISH probes. (bmj.com)
  • Characterisation of Robertsonian translocations in cattle by in situ hybridisation using centromeric probes. (edu.au)
  • Nucleic Acids Res. (idtdna.com)
  • Fleischhacker M, Schmidt B. Circulating nucleic acids (CNAs) and cancer-a survey. (najms.com)
  • Schwarzenbach H, Hoon DS, Pantel K. Cell-free nucleic acids as biomarkers in cancer patients. (najms.com)
  • The EPS matrix is made up of polysaccharides, proteins and nucleic acids. (ukessays.com)
  • Our technique enables identification of driver and prognostic CNAs in archival patient samples previously deemed unsuitable for genomic analysis due to DNA limitations. (biomedcentral.com)
  • Although CNAs varied widely, the subtypes of tumors have characteristic genomic profiles that could facilitate the differential diagnosis of LPS subtypes, especially between WDLPS and DDLPS. (biomedcentral.com)
  • The DDLPS-associated losses were much more common in tumors with increased genomic complexity. (biomedcentral.com)
  • Our findings show that the application of array-CGH allows to delineate clearly the genomic profiles of WDLPS, DDLPS and MLPS that reflect biological differences between these tumors. (biomedcentral.com)
  • The genomic DNA was extracted using the same genomic DNA isolation kit as described above and used in MilliporeSigma's samples. (sigmaaldrich.com)
  • Scanning and image analysis were conducted according to Agilent's Oligonucleotide Array-based CGH for Genomic DNA analysis Protocol (version 4.0). (sigmaaldrich.com)
  • The frequencies of CNVs in these populations were analyzed by oligonucleotide array comparative genome hybridization, quantitative PCR, PCR, DNA sequencing across breakpoints, and single-worm PCR. (biomedcentral.com)
  • High-resolution microarray-based comparative genomic hybridization (CGH) techniques have successfully been applied to study copy number imbalances in a number of settings such as the analysis of cancer genomes. (biomedcentral.com)
  • Evidence-based genomic diagnosis characterized chromosomal and cryptic imbalances in 30 elderly patients with myelodysplastic syndrome and acute myeloid leukemia. (najms.com)
  • This dissertation focuses on computational methods for improving the accuracy of commonly used nucleic acid tests for pathogen detection and diagnostics. (umd.edu)
  • For microarray, novel pan-genomic design and analysis methods are presented for the characterization of unknown microbial isolates. (umd.edu)
  • To demonstrate the effectiveness of these methods, pan-genomic arrays are applied to the study of multiple strains of the foodborne pathogen, Listeria monocytogenes, revealing new insights into the diversity and evolution of the species. (umd.edu)
  • In addition, chromosomes with a high number of linked genes that are conserved, even in distant species, were detected. (metasystems-international.com)
  • Functional analysis of multiple genomic signatures demonstrates that classification algorithms choose phenotype-related genes. (clinbioinfosspa.es)
  • A higher abundance of the differentially expressed genes including those encoding ethylene responsive factors was detected at bulblet initiation stage compared to the late stage of bulblet development. (bvsalud.org)
  • Gene and genome duplications are the primary source of new genes and have played a pivotal role in the evolution of genomic and organismal complexity [ 1 - 4 ]. (biomedcentral.com)
  • CF27 exhibits a large number of genomic islands (mostly containing genes of unknown function), suggesting that a large number of genes has been acquired by horizontal gene transfer over time. (frontiersin.org)
  • For some of the genomic regions that are deleted in some neuroblastomas, on 1p, 3p and 11q, candidate tumor suppressor genes have been identified. (biomedcentral.com)
  • These validated assemblies provide the ultimate nucleic acid diagnostic, revealing the entire sequence of a genome. (umd.edu)
  • We fully expect that focusing in the field of copy number variation will lead to the discovery of the genomic causes of multiple diseases, as well as advanced therapeutic treatment strategies," said Anthony Johnson, president and CEO of Empire Genomics. (analytica-world.com)
  • Copy number variants (CNVs), defined as losses and gains of segments of genomic DNA, are a major source of genomic variation. (biomedcentral.com)
  • Overall, the spectrum of genomic deviations is evidence for metastatic capacity in advanced-disease DTCs and variation in that capacity in DTCs from localized disease. (fhcrc.org)
  • Introduction Recently, genomic research in livestock is focused on genomic variation and its effect on phenotypic performance in economic traits. (ac.ir)
  • It was concluded that the fluorescence ratios obtained were accurate and that differences between genomic DNA from different cell types were detectable, and therefore that CGH was a highly useful cytogenetic analysis tool. (wikipedia.org)
  • Genomic amplifications were observed by CGH in 5 of the 10 cases where gene amplification was detected by Southern blot analysis. (duke.edu)
  • Array comparative genomic hybridization analysis of colorectal cancer cell lines and primary carcinomas. (ox.ac.uk)
  • We sequenced and constructed the complete genome of an environmental strain CR1 of P. aeruginosa and performed the comparative genomic analysis. (frontiersin.org)
  • strain CF27, new sequences were generated, and an update assembly and functional annotation were undertaken, followed by a comparative analysis with other Acidithiobacillus species whose genomes are publically available. (frontiersin.org)
  • Our analysis lays the foundation for elucidation of the relationship between DTC genomic alterations and progressive prostate cancer. (fhcrc.org)
  • Comparative genomic hybridization (CGH) is a powerful technique capable of identifying both gains and losses of DNA sequences. (duke.edu)
  • Unlocking clinically translatable genomic information, including copy number alterations (CNA), from formalin-fixed paraffin-embedded (FFPE) tissue is challenging due to low yields and degraded DNA. (biomedcentral.com)
  • To provide a better understanding of these cells, we have characterized their genomic alterations. (fhcrc.org)
  • We found fewer and less striking genomic alterations in the 48 pools of disseminated cells from patients with organ-confined disease. (fhcrc.org)
  • This is because balanced chromosomal abnormalities such as reciprocal translocations, inversions or ring chromosomes do not affect copy number, which is what is detected by CGH technologies. (wikipedia.org)
  • The authors painted a series of individual human chromosomes from a DNA library with two different fluorophores in different proportions to test the technique, and also applied CGH to genomic DNA from patients affected with either Downs syndrome or T-cell prolymphocytic leukemia as well as cells of a renal papillary carcinoma cell line. (wikipedia.org)
  • In essence, relative ratios of copy number between two DNA samples are obtained by comparing the two fluorescent signal intensities for each probe under the assumption that intensities reflect the amount of corresponding genomic DNA in the respective sample. (biomedcentral.com)
  • Genomic DNA from normal male was obtained from the whole blood of an individual without any history of cancer. (sigmaaldrich.com)
  • We first present an array comparative genomic hybridization method capable of detecting genomic changes in the small number of disseminated cells (10-20) that can typically be obtained from bone-marrow aspirates of prostate-cancer patients. (fhcrc.org)
  • Genomic information for outlier strains of Pseudomonas aeruginosa is exiguous when compared with classical strains. (frontiersin.org)
  • Wallaby is detected in Penicillium collections exclusively in strains from food environments. (nature.com)
  • In order to identify and compare the genomic profiles, we analyzed array-based comparative genomic hybridization (array-CGH) profiles of 66 liposarcomas, including well-differentiated (WDLPS), dedifferentiated (DDLPS) and myxoid (MLPS) subtypes. (biomedcentral.com)
  • Sequencing of the two leading filamentous fungi used in cheese making, P. roqueforti and P. camemberti , and comparison with the penicillin producer P. rubens reveals a 575 kb long genomic island in P. roqueforti -called Wallaby -present as identical fragments at non-homologous loci in P. camemberti and P. rubens . (nature.com)
  • A later application was the use of random sequence primers to detect random amplified polymorphisms (RAPDs) [ 3,4 ]. (idtdna.com)
  • A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
  • CGH is only able to detect unbalanced chromosomal abnormalities. (wikipedia.org)
  • Results are presented that show these "signature" sequences can be used to detect pathogens in complex samples and differentiate them from their non-pathogenic, phylogenetic near neighbors. (umd.edu)
  • He thanked the regional and global specialized laboratories for their efforts to provide timely results of the genomic sequencing of wild polioviruses, and for their help in planning and monitoring polio eradication activities in the Region. (who.int)
  • Comparative studies of mammalian estrogen receptors from tissue culture and rats. (edu.au)
  • The genomic DNA was extracted using the GenEluteā„¢ Mammalian Genomic DNA Miniprep Kit (Product G1N70) and was used in MilliporeSigma's samples. (sigmaaldrich.com)
  • We identified eight new regions of genomic plasticity and a plasmid pCR1 with a VirB/D4 complex followed by trimeric auto-transporter that can induce virulence phenotype in the genome of strain CR1. (frontiersin.org)
  • These hotspots of primate CNV formation provide a novel perspective on divergence and selective pressures acting on these genomic regions. (biomedcentral.com)
  • These genomic regions appear to have an elevated likelihood of positive selection, based on nucleotide level conservation and transcriptional data. (biomedcentral.com)
  • This paper will focused on detecting of CNV in sheep genome. (ac.ir)
  • Here, we show, based on genomic, morphometric, ecophysiological and mating compatibility data, an example of ecotypic differentiation and speciation within an endemic pelagic inhabitant, the diatom Fragilariopsis kerguelensis. (metasystems-international.com)
  • Hence, we provide evidence that the immortalization strategy that we applied is capable of detecting cell heterogeneity in the NP. (biomedcentral.com)
  • Agilent CGH Analytics software (v3.4) was used to visualize, detect and analyse aberration patterns from CGH microarray profiles. (sigmaaldrich.com)
  • The effects of CMTM4 on cellular apoptosis and cell cycle progression were analysed by flow cytometry, and cell cycle hallmarks were detected by western blotting and RT-PCR. (biomedcentral.com)
  • 6, 33 Case 7, with an interstitial deletion of 15q24, was serendipitously detected during subtelomeric screening, as the 15q control probe (LSI PML) in the subtelomeric screening kit (Vysis Inc, Downers Grove, IL) was deleted. (bmj.com)
  • Male and female genomic DNAs were intermittently sonicated using a sonicator probe (Digital Sonifier 450, Branson Ultrasonics Corp., CA) for 5 sec, 30 sec, 90 sec, or 120 sec. (sigmaaldrich.com)