AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaTechnology, Industry, AgricultureInformation ScienceHealth Care
Gas Chromatography-Mass SpectrometryMass SpectrometryChromatography, GasChromatography, LiquidChromatography, High Pressure LiquidVolatile Organic CompoundsSolid Phase MicroextractionMetabolomicsTandem Mass SpectrometryMetabolomePerianal GlandsSpectrometry, Mass, Electrospray IonizationOils, VolatileVolatilizationOcimum basilicumChromatography, Thin LayerAndrostenediolsAcetic AnhydridesSubstance Abuse DetectionMeconiumDeuteriumDoping in SportsF2-IsoprostanesMagnetic Resonance SpectroscopyFatty AcidsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationBiotransformationCarboxylic AcidsHydroxyeicosatetraenoic AcidsGasesUrinalysisReproducibility of ResultsOxidation-ReductionEstersQuality ControlSteroidsMethylationMolecular StructureCarbohydrate ConformationWater Pollutants, ChemicalCarbohydrate SequenceLipoxygenaseMolecular Sequence DataPolycyclic Hydrocarbons, AromaticStereoisomerismSensitivity and SpecificityChemistryChemical PhenomenaArachidonic AcidsArachidonic AcidHydrolysisProteomicsDinoprostCytochrome P-450 Enzyme SystemFatty Acids, UnsaturatedBiological MarkersLiverHexanesChromatography, AffinityTime FactorsCattleChromatography, Ion ExchangeKineticsEucommiaceaeCalibrationAmino Acid SequenceLipid MetabolismIsotope LabelingPentanesChromatography8,11,14-Eicosatrienoic AcidReference StandardsProteomeLimit of DetectionChromatography, GelRhodiolaBreath TestsReference ValuesWater PollutantsFlame IonizationSugar AcidsCarbon RadioisotopesCarbon IsotopesChromatography, Reverse-PhaseMethanolGlucoseDose-Response Relationship, DrugEnvironmental MonitoringCholesterolOligosaccharidesSugar AlcoholsOxidative StressAcetatesMicrosomes, LiverSulfur CompoundsPeptidesFungicides, IndustrialTerphenyl CompoundsSolid Phase ExtractionAmino Acids
Tandem mass spectroSpectroscopyCapillaryQuantificationChromatographicHPLCCompoundsPyrolysisDerivatizationAqueousInjectionAntimicrobialUrineDetectorMetabolicMoleculesCharacterizationLiquidIsotopeDeterminationMetabolitesQuantitativeExtractionCrudeAnalytical MethodThermalMercurySpectraTechniquesSamplesAnalysisChemicalValidationPracticalGlycidolInvolvesProcedure
Tandem mass spectro2
  • The analysis of the targeted compounds was carried out by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry, using a triple quadrupole analyzer (UHPLC-MS/MS-QqQ). (bvsalud.org)
  • This bachelor thesis deals with the qualitative analysis of selected subclasses of glycosphingolipids isolated from human blood plasma using hydrophilic interaction liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HILIC-ESI-MS/MS). Glycosphingolipids are ubiquitous and structurally very diverse components of cell membranes involved in many cellular functions. (nusl.cz)
Spectroscopy3
  • The method was established based on the stable isotope-dilution liquid chromatography-tandem mass spectroscopy (ID-LC-MS/MS) technique. (biomed.news)
  • Structures 1-3 of the new compounds were elucidated by nuclear magnetic resonance (NMR) and NMR spectroscopy, but mass spectrometry (MS) techniques and their absolute configuration were determined by density functional theory (DFT) calculations and Mosher derivatisation. (uky.edu)
  • We also encourage people working with all techniques (IRMS-, NMR- or spectroscopy-based) to present advances in methods, as well as researchers focusing on improving oxygen and hydrogen isotope-based models to discuss their approaches. (copernicus.org)
Capillary3
  • 1) in the first part of the study, the analytical methods examined were isocratic high pressure liquid chromatography (HPLC) with ultraviolet (UV) detection, capillary gas chromatography (GC) with temperature programming and flame ionisation detection and gas chromatography-mass spectrometry (GC-MS). Twelve NSAIDs in common use in the UK were selected as test compounds for the evaluation of the separating ability, selectivity and sensitivity of detection of the three methods. (gla.ac.uk)
  • The fibre with the extracted carbamates was injected into the heated split-splitless injection port of the gas chromatograph where the analytes were evaporated at 300 degrees C, separated on a methylsilicone capillary column and detected by either a nitrogen phosphorous detector or by mass spectrometry. (erowid.org)
  • Analyses of uraemic molecules in serum sample of a group of patients suffering from CKD were performed with three analytical methods including capillary electrophoresis‚ÄövÑv™mass spectrometry (CE‚ÄövÑv™MS), gas chromatography‚ÄövÑv™mass spectrometry (GC‚ÄövÑv™MS) and liquid chromatographymass spectrometry (LC‚ÄövÑv™MS), and the results compared with a healthy (control) group. (edu.au)
Quantification10
  • Because auxins and their metabolites occur at trace levels in plant tissue, experiments requiring identification plus their selective and specific quantification can be most conveniently achieved using mass spectrometry (MS) and the associated chromatographic methods. (bio-thing.com)
  • This study aims to develop a convenient quantification method for serum MMA. (biomed.news)
  • Example MeCAT Workflow Ultra sensitive absolute and relative MeCAT protein quantification is performed by ICP-MS (inductively coupled plasma mass spectrometry) on protein level down to low attomol range. (mecat.eu)
  • After that they are analysed with state-of-the-art mass spectrometric methods including ICP-MS with its extraordinary advantages in quantification. (mecat.eu)
  • ICP-MS (inductively coupled plasma mass spectrometry) allows an ultra sensitive (absolute) quantification of the metals (lanthanides) tagged to proteins on protein level . (mecat.eu)
  • ICP-MS has several unrivaled advantages in quantification compared to 'classical' bioanalytical mass spectrometry. (mecat.eu)
  • Quantification of major components by gas chromatography with flame-ionization detector (GC-FID) is established in routine laboratory practice. (nusl.cz)
  • Robust and standardised methods for the quantification of cannabinoids within botanical and drug forms is a critical step forward for an emerging Cannabis-based pharmaceutical industry, which is poised for rapid expansion. (researchgate.net)
  • Despite a growing body of analytical methods for the quantification of cannabinoids, few have been validated using internationally accredited guidelines. (researchgate.net)
  • Validation parameters for establishing robust standardised methods for cannabinoid quantification within Cannabis-based drug forms are critically discussed. (researchgate.net)
Chromatographic1
  • To achieve sufficient retention and separation of a broad range of metabolites with diverse chemical structures and physicochemical properties, LC-MS/MS based targeted metabolomics often involves 3 complemented chromatographic separation methods, including reversed-phase liquid chromatography (RP-LC), hydrophilic interaction liquid chromatography (HILIC), and ion-pair liquid chromatography (IP-LC). (biomed.news)
HPLC1
  • In addition, fluorescence spectrometry and electrochemical detection (ECD) were examined to assess their value as additional HPLC detection systems which might increase the selectivity or sensitivity of the method. (gla.ac.uk)
Compounds10
  • Pyrolysis gas chromatography is useful for the identification of involatile compounds. (wikipedia.org)
  • Depending on the method used, and the possible interfering compounds present, further cleanup and preparative steps may be included. (cdc.gov)
  • All of the test compounds were eluted from the column and could be detected by UV spectrometry at a sample size of 5 nanograms on-column. (gla.ac.uk)
  • Five of the test compounds could be detected by fluorescence spectrometry and seven by ECD. (gla.ac.uk)
  • Three compounds, all pyrazolidine diones, could be chromatographed without derivatisation but produced a mixture of products following reaction with the methylating agent, diazomethane. (gla.ac.uk)
  • The back pressure of the system was set to 1.38 × 107 Pa and the temperature to 45°C. The six compounds were separated within 11 min by the proposed ultra-high performance supercritical fluid chromatography method with satisfactory resolution. (bvsalud.org)
  • Therefore, this work provides a simple and novel method for the simultaneous analysis of six compounds in Guizhi Fuling capsule and tablet samples. (bvsalud.org)
  • The FID detector is considered as universal detector for gas chromatography (GC), but it does not provide a response for a wide range of compounds and GC-FID analysis provides only quantitative data. (nusl.cz)
  • Crude oil and unprocessed gas condensates contain significant amounts of suspended mercury compounds mainly mercuric sulfide. (researchgate.net)
  • 25 Similarly, studies have shown that maintenance and inspection workers in oil and gas operations are exposed to high levels of mercury and its related compounds through inhalation and dermal absorption, which poses grave risks to their health and safety. (researchgate.net)
Pyrolysis2
Derivatization1
Aqueous1
  • Phenolic content of bee bread has been quantified in methanol and aqueous extracts using Folin-Ciocalteu (FC) method[ 11 ]. (ijpsonline.com)
Injection2
  • The purpose of this study is to quantitatively evaluate intra-batch variations or injection order effects of the RP-LC, HILIC, and IP-LC methods for targeted metabolomics analyses, and develop strategies to minimize intra-batch variations and correct injection order effects for problematic metabolites. (biomed.news)
  • The IP-LC method exhibits significant injection order effects, which could be effectively corrected by the developed exponential models of signal drift trends as a function of injection order for individual targeted metabolites. (biomed.news)
Antimicrobial1
  • We present the antidiabetic, anticancer, antimicrobial, hepato-, and neuroprotective activities of p-MCA and methods of its lipophilization that have been developed so far to increase its industrial application and bioavailability in the biological systems. (bvsalud.org)
Urine4
  • The determination of amphetamines and their methylenedioxylated analogs in urine by propylchloroformate derivatisation and automated solid-phase microextraction is described. (erowid.org)
  • The method was shown to be highly reproducible and robust with respect to variations in the urine matrices. (erowid.org)
  • The method is a solvent free, automated alternative to traditional methods for determination of the amphetamine and their methylendioxylated analogs in urine. (erowid.org)
  • Simultaneous quantitation of ephedrines in urine by gas chromatography-nitrogen-phosphorus detection for doping control purposes. (ugent.be)
Detector1
  • The most common currently used method of analyzing for the presence of sulfur mustard and its metabolites in biological and environmental samples is gas chromatography/mass spectrometry (GC/MS). Prior to 1987, however, thin-layer chromatography (TLC) with a colorimetric detection system and gas chromatography with either flame ionization detector (FID), electron capture detector (ECD), or flame photometric detector (FPD) were the most frequently used methods. (cdc.gov)
Metabolic5
  • The 13C distribution in intracellular metabolites can be detected via mass spectrometry and used as a constraint in intracellular metabolic flux calculations. (biomed.news)
  • Therefore, it is essential to correct the mass spectrometry data before performing metabolic flux calculations. (biomed.news)
  • The method employed a basic power method for constructing the correction matrix with simple structure and easy coding implementation, which can be directly applied to data pre-processing in 13C metabolic flux analysis. (biomed.news)
  • The correction method was then applied to the intracellular metabolic flux analysis of 13C-labeled Aspergillus niger. (biomed.news)
  • The results showed that the proposed method was accurate and effective, which can serve as a reliable data correction method for accurate microbial intracellular metabolic flux analysis. (biomed.news)
Molecules1
  • This makes the isotopic information of elements other than the 13C marker interspersed in the isotopic distribution measured by the mass spectrometry, especially that of the molecules containing many other elements, which leads to large errors. (biomed.news)
Characterization1
  • The invention regards a method which enables high reproducible systematic, qualitative and quantitative characterization of proteomes and biomolecules. (mecat.eu)
Liquid1
  • This method is applicable to solid and liquid fats and oils. (iteh.ai)
Isotope1
  • In this paper, we proposed a method for construction of correction matrix based on Python language for correcting the measurement errors due to natural isotope distribution. (biomed.news)
Determination2
  • A fast and simple ultra-high performance supercritical fluid chromatography method has been developed for the determination of six analytes, namely (paeonol, coumarin, cinnamic alcohol, cinnamic acid, paeoniflorin, and amygdalin) in Guizhi Fuling capsule and tablet samples. (bvsalud.org)
  • Furthermore, methods of isolation, purification and analytical possibilities for the determination of glycosphingolipids in biological samples are described here.In the experimental part, blood samples were taken and processed together with the preparation of the necessary solutions, plasma samples, and standards. (nusl.cz)
Metabolites3
  • The purpose of this chapter is to describe the analytical methods that are available for detecting, measuring, and/or monitoring sulfur mustard, its metabolites, and other biomarkers of exposure and effect to sulfur mustard. (cdc.gov)
  • Table 7-1 summarizes several representative analytical methods for detecting sulfur mustard and its metabolites in biological samples. (cdc.gov)
  • The primary method of analyzing for sulfur mustard exposure is by detecting the presence of its hydrolysis metabolites in biological fluids. (cdc.gov)
Quantitative1
  • The coupling of gas chromatography with mass spectrometry is a powerful analytical tool that provides not only quantitative data but also qualitative data on the structure of substances. (nusl.cz)
Extraction3
  • An extraction method based on the use of solvents (tert-butyl methyl ether (20% ethyl acetate, v/v)) was carried out and cleaning of the extract with a mixture of sorbents (Si-SAX, PSA and Z-sep+) was optimized for the elimination of fatty interferents. (bvsalud.org)
  • The study aimed to establish the detection method for bound 3-, 2-MCPD, and glycidol using accelerated solvent extraction (ASE) and gas chromatography mass spectrometry (GC-MS). The ASE was modified for reduced solvent volume and process time to extract lipid from the chocolate spread, infant formula, potato chips, and sweetened creamer. (bvsalud.org)
  • Moreover, a test analysis, optimization of mobile phase gradient and comparison of the most frequently used extraction methods according to Folch, Bligh and Dyer, Matyash, and Alshehry alongside fractionation of lipid extract is described in this part. (nusl.cz)
Crude2
  • There are several mercury species present in crude oil and gas condensates that are seldom accounted for in routine analysis. (researchgate.net)
  • The download humanism and early modern philosophy (london studies in the history differs in the international spectrometry of crude franchise, which is for physical device over focus students. (ournest.com)
Analytical Method1
  • In order to address potential barriers to analytical method standardisation, a collaborative research initiative inclusive of academic and commercial stakeholders is proposed. (researchgate.net)
Thermal1
  • The facility boasts a wide range of equipment from multiple manufacturers covering instrumentation in gas chromatography such as comprehensive multi-dimensional gas chromatography, PTV injectors, autosamplers, thermal desorbers and pyrolysers. (anthias.co.uk)
Mercury8
  • 18,19 Similarly, mercury exposure poses a significantly higher threat to the oil and gas industry when compared to other hazards. (researchgate.net)
  • 1 Furthermore, studies have shown that the exposure of oil and gas workers to high levels of mercury could result in acute and chronic illnesses depending on the type, concentration, and exposure duration of mercury. (researchgate.net)
  • 20, 21 However, mercury is not only hazardous to human health but also detrimental to gas processes and equipment. (researchgate.net)
  • The occurrence of mercury and its toxic derivatives is considered one of the many negative impacts of oil and gas operations. (researchgate.net)
  • This study examines the occurrence, exposure, and symptoms of mercury on the health and safety of oil and gas workers in Oman. (researchgate.net)
  • It also explores the acute/chronic effects of mercury poisoning on the maintenance and inspection workers, who are more prone to the adverse effects of mercury poisoning during oil and gas operations. (researchgate.net)
  • Methods: The two-pronged approach of literature review and survey questionnaire was used to deduce the effects of mercury exposure and poisoning using 68 respondents with 1 - 6 years of working experience in the sector. (researchgate.net)
  • However, the respondents who experienced methyl mercury (MeHg) symptoms are due to consuming seafood and cigarettes, whereas elemental and organic mercury symptoms are due to oil and gas operations. (researchgate.net)
Spectra1
  • This message indicates backward compatibility problems in GMD's mass spectra, chemical compound structure and metabolite profile visualizations. (mpg.de)
Techniques2
  • In particular, the participants received insights into the techniques, such as gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The training also covered methods for sample preparation and derivatisation. (opcw.org)
  • Thus the protein amount can be accurately determinated down to low attomol range which is at least 2 to 3 orders of magnitude more sensitive than other mass spec (peptide based) techniques. (mecat.eu)
Samples2
  • Many of the analytical methods used for environmental samples are the methods approved by federal agencies and organizations such as EPA and the National Institute for Occupational Safety and Health (NIOSH). (cdc.gov)
  • Bee bread samples of stingless bee ( Heterotrigona itama ) collected from Kelantan (east coast), Selangor (central) and Perak (northern) regions of Malaysia were subjected to nutritional analysis using standard hydrolysis and oxidation methods. (ijpsonline.com)
Analysis4
  • Rather, the intention is to identify well-established methods that are used as the standard methods of analysis. (cdc.gov)
  • The development and validation of a method for the analysis of traces of 3-monochloropropanediol (3-MCPD) esters (19) and glycidyl esters (7) of fatty acids in vegetable oils, margarine, biscuits and croissants was performed. (bvsalud.org)
  • Faeces were collected quantitatively over a 5 d period for analysis of neutral sterols and bile acids by gas chromatographically methods. (biomedcentral.com)
  • Traditional methods of wine analysis are often expensive, time consuming, require either elaborate equipment or specialist expertise and frequently lack accuracy. (megazyme.com)
Chemical1
  • No specific EPA, NIOSH, or AOAC methods were found for this chemical. (cdc.gov)
Validation1
  • Method validation confirmed that the procedure is accurate with the recovery rates from 87.04 to 104.30%, intraday precision values less than 4.81% and interday precision less than 5.22%, and linear with R2 higher than 0.9967. (bvsalud.org)
Practical1
  • The Open University provides Anthias Consulting with the Training Facility for the high-quality, affordable, instrument-independent training courses that can boost your knowledge, confidence and results in all aspects of gas chromatography and GC-MS. These courses have a strong practical focus which can be applied the instant you return to your lab and are applicable to all levels of experience. (anthias.co.uk)
Glycidol2
  • The method is based on alkalinecatalyzed ester cleavage, transformation of the released glycidol into monobromopropanediol (MBPD) and derived free diols (MCPD and MBPD) with phenylboronic acid (PBA). (iteh.ai)
  • La méthode repose sur un clivage des esters par catalyse alcaline, une transformation du glycidol ainsi libéré en monobromopropanediol (MBPD) et une dérivatisation des diols libres (MCPD et MBPD) en présence d'acide phénylboronique (PBA). (iteh.ai)
Involves1
  • The method involves the use of the eco-friendly difluoromethylating agent diethyl (bromodifluoromethyl) phosphonate (DBDFP) and results in the efficient tagging of the hydroxyl group in the phenols with the difluoromethyl (CF2H) moiety. (deepdyve.com)
Procedure1
  • It was concluded that GC-MS was the only method which would have sufficient selectivity and separating ability for use in a screening procedure and this method was used in the rest of the study. (gla.ac.uk)