Data gene transfer techniques. Medical search. Frequent questions

The research team employed bacterial conjugation-the transfer of genetic material between bacterial cells-to allocate Mobile-CRISPRi from common laboratory strains into diverse bacteria, even including a little-studied microbe making its home on cheese rinds. (genengnews.com)
Taxonomists classify these organisms into species in much the same way as they classify eukaryotes, but differences in their biology-including horizontal gene transfer between distantly related taxa and variable rates of homologous recombination-mean that we still do not understand what a bacterial species is. (ox.ac.uk)

Comparative analysis of the transcriptomes from the 3 different conditions identifies 45 differentially expressed genes during oval cell regeneration, 27 up-regulated and 18 down-regulated. (uni-goettingen.de)
Quantitative PCR and high-throughput methods, such as expressed sequence tag analysis, have been used for measuring gene expression and identifying subsets of elm genes that are differentially expressed in the presence of O. novo-ulmi . (sisef.it)
These analyses have also helped identify genes that were differentially expressed in DED fungi grown under defined experimental conditions. (sisef.it)

Only those strains with tfdA sequences highly similar to the tfdA sequence of strain JMP134 (tfdA class I) transferred all the 2,4-D genes and conferred the 2,4-D degradation phenotype to a Burkholderia cepacia recipient. (nih.gov)
Because these approaches will rely on novel data types such as DNA sequences and high-throughput phenotyping images, Breeding 4 will call for analyses that are complementary to traditional quantitative genetic studies, being based on machine learning techniques which make efficient use of sequence and image data. (springer.com)
It may also be the case that gene editing -- which largely involves DNA alterations that could possibly happen in nature -- is less inherently 'scary' than transgenic techniques transferring DNA sequences between unrelated species," Lynas added. (sciencedaily.com)
BMS-265246 Gene transfer, accomplished by transposition, happens by a cut-and-paste mechanism (Ivics gene transfer applications, where one goal is definitely to accomplish life-long appearance of the launched gene sequences. (ecolowood.com)
The main expectation of gene delivery studies using the SB transposon system is definitely to accomplish genomic integration of transposon-encoded sequences. (ecolowood.com)

Our project " AMRflows: antimicrobials and resistance from manufacturing flows to people: joined up experiments, mathematical modelling and risk analysis " uniquely combines field sampling, mesocosm and laboratory studies with mathematical modelling of the population dynamics of sensitive versus resistant bacteria, plasmid transfer and selection by antibiotics and metals and mathematical modelling of water transport through the environment. (bham.ac.uk)
While many studies have decorticated the mechanisms of mobility in model plasmids, the identification and characterization of plasmid mobility from genome data are unexplored. (pasteur.fr)
T4CPs, relaxases, and VirB4, are at the core of plasmid conjugation, and together with accessory genes, they have evolved into specific systems adapted to specific physiological and ecological contexts. (pasteur.fr)
Our data recommend that SB delivery via plasmid in Testosterone levels cells BMS-265246 should end up being transported out with extreme care because of suddenly high duplicate quantities of arbitrarily integrated SB transposase. (ecolowood.com)
Be able to describe the function of 2 important genes on the pBestLuc plasmid. (scienceteacherprogram.org)
Gene therapy involves viral, plasmid, and oligonucleotide-based approaches. (medscape.com)

Researchers have succeeded in altering HIV particles to repair human genomes in a process known as the "hit-and-run" technique. (rt.com)
The HGT creates widespread discordance between evolutionary histories of different genes as genomes become mosaics of gene histories. (blogspot.com)
Many RNA species have been found in eukaryotic cells by interpretations of gene transcription within genomes. (biomedcentral.com)
Cupin evolution is discussed from the perspective of the structure-function relationships, using data from the genomes of several prokaryotes, especially Bacillus subtilis. (embl.de)
The ongoing annotation of these genomes should spark a new wave of interest in the DED pathosystem, as it should lead to the formal identification of genes modulating parasitic fitness. (sisef.it)

Chemical analogues and compounds with biological or physical characteristics similar to those of suspected carcinogens may also be considered, even in the absence of data on a possible carcinogenic effect in humans or experimental animals. (who.int)

GAA gene transfer with adeno-associated virus (AAV) vectors has been shown to reduce glycogen storage and improve the PD phenotype in preclinical studies following different approaches. (nih.gov)
Here, we present an overview of the different gene therapy approaches for PD, focusing on in vivo gene transfer with AAV vectors and discussing the potential opportunities and challenges in developing safe and effective gene therapies for the disease. (nih.gov)
Based on emerging safety and efficacy data from clinical trials for other protein deficiencies, in vivo gene therapy with AAV vectors appears to have the potential to provide a therapeutically relevant, stable source of GAA enzyme, which could be highly beneficial in PD. (nih.gov)
Plasmids are key vectors of horizontal gene transfer and essential genetic engineering tools. (pasteur.fr)
This platform is used to produce viral vectors for gene transfer, both in vivo and in vitro. (lu.se)

Instead, it just sits on the DNA, blocking other proteins from gaining access to and turning on a particular gene. (genengnews.com)
a map is constructed that shows interactions among molecular entities (such as genes, proteins and RNAs), using information from literature and databases. (hindawi.com)

Temporal regulation of these genes in the context of the oval cell regeneration was studied by Real Time PCR. (uni-goettingen.de)
Kinetically down-regulated genes involved in the lipid metabolism are: hydroxyacid oxidase 3, fatty acid CoA ligase long chain 2, and steroyl CoA desaturase 1.The regulation of protein expression of the cell cycle genes CDC42 and cyclin D1 was studied by Western Blot technique, in the oval cell liver regeneration. (uni-goettingen.de)
Although microRNAs (miRNAs) are physically small, they have been shown to play an important role in gene regulation [ 1 ]. (hindawi.com)
The application of the systems biology approach to the analysis of a gene regulatory network is demonstrated with a case study of the regulation of p21 by multiple miRNAs [ 4 ]. (hindawi.com)
ExRNAs may be transferred between cells and have functional significance in health and diseases by horizontal gene regulation. (biomedcentral.com)
The construction of gene regulatory networks (GRNs) is vital for understanding the regulation of metabolic pathways, biological processes, and complex traits during plant growth and responses to environmental cues and stresses. (mtu.edu)
Employing transfer learning techniques, this study has also successfully transferred the known knowledge of gene regulation from one species to another, substantially improving performance and manifesting the viability of cross-species learning using deep learning-based approaches. (mtu.edu)

The new technique, known as Mobile-CRISPRi, harnesses the power of CRISPR interference-which blocks gene expression through the use of a catalytically inactive Cas9 protein (dCas9) and programmable single guide RNAs-allowing scientists to screen for antibiotic function in a wide range of pathogenic bacteria. (genengnews.com)
The result is a lower expression of the gene and a reduced amount of the protein it codes for. (genengnews.com)
For the purpose of choosing prognostic genes, the expression of oxidative stress-related genes (OXEGs) was found. (hindawi.com)
Oval cells proliferate and differentiate in hepatocytes expressing albumin, and they differentiate in biliary cells expressing cytokeratin 7 (CK 7).Serial Analysis of Gene Expression (SAGE) was applied to the hepatic stem cell model of rat liver regeneration for studying gene expression pattern of liver regeneration during early stage of oval cell proliferation and differentiation. (uni-goettingen.de)
Results: Cytokeratin gene expression increased in all samples, when as few as 10 tumour cells were added. (iiarjournals.org)
In this regard, we used blood samples from a healthy donor mixed with different numbers of established breast cancer cells (CAMA-1, MCF7, ZR-75-1) and carried out subsequent gene expression analysis for cytokeratins 8, 18 and 19. (iiarjournals.org)
MicroRNAs (miRNAs) are potent effectors in gene regulatory networks where aberrant miRNA expression can contribute to human diseases such as cancer. (hindawi.com)
For a better understanding of the regulatory role of miRNAs in coordinating gene expression, we here present a systems biology approach combining data-driven modeling and model-driven experiments. (hindawi.com)
This work implies that, to achieve comparable gene expression in the injured liver, higher adenoviral titers may be required, an important consideration as gene therapy in disease states is considered. (duke.edu)
While the selective inactivation of Ophiostoma genes by insertional mutagenesis remains a challenge, an alternative approach based on RNA interference is now available for down-regulating the expression of targeted genes. (sisef.it)
Expression of the cystic fibrosis transmembrane conductance regulator gene ( CFTR ) can be detected in the pancreatic ductules at 18 weeks' gestation. (medscape.com)

Horizontal gene transfer. (matec-conferences.org)
In prokaryotes, however, the vertical signal is partly obscured by the massive influence of horizontal gene transfer (HGT). (blogspot.com)

For far too long, researchers have lacked the essential tools to connect the dots between pathogenic genes and phenotypes. (genengnews.com)
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)

The study was published after analyzing data from a five-year period between January 2018 and December 2022. (sciencedaily.com)
As of first of January 2022, StemTherapy and MultiPark have decided to merge the former iPSC, CRISPR and vector platforms into the new Cell and Gene Therapy Core . (lu.se)

gene therapy. (nih.gov)
Shares in two US gene therapy biotechs have fallen after two cutting-edge treatments failed to produce convincing results in early clinical trials. (pharmaphorum.com)
In a separate announcement Solid Biosciences, from Cambridge, Massachusetts, said that it is trying to increase the dose of its SGT-001 gene transfer therapy for Duchenne muscular dystrophy. (pharmaphorum.com)
Transarterially administered gene therapy for primary and secondary liver malignancies. (aetna.com)
Therapeutic strategies for the dystrophinopathies can be categorized into 3 groups based on their approach: (1) Supportive pharmacologic therapy, (2) research gene therapy, and (3) research cellular therapy. (medscape.com)
Cell therapy uses myoblast and stem cell techniques. (medscape.com)
This increase poses important problems, including a) the lack of available antimicrobial therapy for VRE infections, because most VRE are also resistant to drugs previously used to treat such infections (e.g., aminoglycosides and ampicillin), and b) the possibility that the vancomycin-resistant genes present in VRE can be transferred to other gram-positive microorganisms (e.g. (cdc.gov)
The Cell and Gene Therapy core is an open-access infrastructure and our services include AAV and LV vector production, cloning services, iPS reprogramming, iPS-edits and CRISPR experimental designs. (lu.se)

Moreover, the system reduces the production of protein from targeted genes, allowing researchers to identify how antibiotics inhibit the growth of pathogens. (genengnews.com)
In the current study, the researchers showed that if they decreased the amount of protein targeted by an antibiotic, bacteria became much more sensitive to lower levels of the drug-evidence of an association between gene and drug. (genengnews.com)
The purpose of the current study is to give gene-modified T cells in combination with gene-modified stem cells to reprogram the immune system to recognize and kill cancer cells that have the NY-ESO-1 protein with sustained killing activity. (ca.gov)

A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
Gene modification of cells involves the transfer of foreign genetic material (DNA) into a cell, in this case the immune system cells and stem cells. (ca.gov)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)

By reviewing the available data and literature, we established a computational protocol to identify and classify conjugation and mobilization genetic modules in 1,730 plasmids. (pasteur.fr)

To make CRISPRi mobile, the researchers developed methods to transfer the system from common lab models like E. coli to disease-causing species, which are often harder to study. (genengnews.com)
2) Using aseptic technique, transfer a loopful of E. coli from the culture tube to your tube of calcium chloride. (scienceteacherprogram.org)

In this review, we will overview of the characterization of exRNA, summarize the current cognition about the role of exRNAs in renal diseases and compare the isolation and detection techniques. (biomedcentral.com)
Genetic and molecular analyses have led to the identification, cloning and characterization of a few genes that contribute to parasitic fitness in the pathogens. (sisef.it)

The improved vector system should prove useful for efficient gene transfer in vertebrates. (mdc-berlin.de)

https://en.wikipedia.org/wiki/Borders_Group (accessed on July 14, 2019). (matec-conferences.org)
https://en.wikipedia.org/wiki/Power_law (accessed on July 14, 2019). (matec-conferences.org)
https://en.wikipedia.org/wiki/Intergalactic_Computer_Network (accessed on July 14, 2019). (matec-conferences.org)
https://en.wikipedia.org/wiki/Timeline_of_virtualization_development (accessed on July 14, 2019). (matec-conferences.org)

Launch The (SB) transposon program provides a non-viral technique of mediating steady transgene reflection in mammalian cells and tissue (Ivics superfamily, comprises of two elements: the catalytic transposase and a transposon encoding any DNA freight. (ecolowood.com)

anti-apoptotic enzyme gene like thioredoxin like 2. (uni-goettingen.de)
Sangamo had been using a technique known as "zinc fingers" to edit patients' DNS, allowing liver cells to produce the enzyme known as IDS. (pharmaphorum.com)
But the company said further data due later this year would show whether the changes in toxic glycosaminoglycan levels, and leukocyte IDUA enzyme activity, will produce meaningful benefits in the patients. (pharmaphorum.com)
The pckA gene, encoding the gluconeogenic enzyme phosphoenolpyruvate carboxykinase (PEPCK), was cloned by PCR amplification from the purple nonsulfur bacterium Rhodopseudomonas palustris No. 7. (asm.org)

Adenovirus-mediated gene transfer has become an important tool with which to introduce genetic material into cells. (duke.edu)

Sequencing of a 2.5-kb chromosomal Sma I- Pst I fragment containing the structural gene revealed an open reading frame encoding 537 amino acids, homologous to known pckA genes. (asm.org)

Thousands of genes at a time can be screened as potential antibiotic targets this way, helping scientists learn how antibiotics work and how to improve them. (genengnews.com)
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. (yeastgenome.org)
The increasing availability of public databases has facilitated the development of numerous methods for inferring gene regulatory relationships between transcription factors and their targets. (mtu.edu)

Looking to the future, the pairing of integrative taxonomy approaches with multi-locus genomic sequencing and phenomic techniques, such as transcriptomics and proteomics, holds great potential to resolve many unknowns in fungal taxonomic classification. (frontiersin.org)
The gene and cell approaches are more likely to be curative, but they are still under investigation. (medscape.com)

A comparison of the dendrogram of the tfdA genes with that of the SSU rDNA genes demonstrated incongruency in phylogenies, and hence 2,4-D degradation must have originated from gene transfer between species. (nih.gov)
The resulting data may help to discriminate among the many theories of prokaryotic species that have been produced to date. (ox.ac.uk)
However, there is limited research on supervised learning techniques that utilize available regulatory relationships of plant species in public databases. (mtu.edu)
Challenges arise due to limited training data for gene regulatory pairs, especially in less-studied species such as poplar and maize. (mtu.edu)
This study contributes to the methodology for growing body of knowledge in GRN prediction and construction for plant species, highlighting the value of adopting hybrid models and transfer learning techniques. (mtu.edu)

Next, several quantitative potency measures for AC3 were implemented, and data were correlated with the physicochemical analyses on thermally stressed and control samples. (bvsalud.org)

PURPOSE: A new universal tool for specific, non-covalent and non-destructive attachment of a recombinant antibody fragment to a polymer-modified adenovirus has been utilised to regulate the tropism of adenoviral gene delivery vector. (ox.ac.uk)

We first construct a p21 regulatory network based on data from the literature and further expand it using algorithms that predict molecular interactions. (hindawi.com)
They code for genes involved in many aspects of microbial biology, including detoxication, virulence, ecological interactions, and antibiotic resistance. (pasteur.fr)
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. (yeastgenome.org)

The values of parameters in this model can be determind from literature, databases or they are directly estimated from quantitative experimental data using optimization methods. (hindawi.com)
Finally, the integration of quantitative data and modeling helped us to generate and validate hypotheses about mechanisms of collective miRNA repression on p21. (hindawi.com)
Detailed, quantitative evaluations of epidemiological data may be made in the Monographs , but without extrapolation beyond the range of the data available. (who.int)
Quantitative extrapolation from experimental data to the human situation is not undertaken. (who.int)

BSc Microbiology has a strong practical basis, and you will learn a wide variety of techniques such as microbial genetics, biochemistry and propagation. (reading.ac.uk)

Available data emphasize efficient adenoviral transduction of parenchymal liver cells (i.e., hepatocytes) in both in vitro and in vivo model systems, typically in normal cells. (duke.edu)

This phase is characterized by the development of methods for biological design of plant varieties, including transformation and gene editing techniques directed toward causal loci. (springer.com)
Such an approach is characterized by an iterative process, including biological data acquisition and integration, network construction, mathematical modeling and experimental validation. (hindawi.com)
depending on the biological problem investigated and experimental data available, the interaction map can be translated into a detailed mechanistic model that can simulate the temporal evolution of molecular entities. (hindawi.com)
In turn, these hypotheses, which provide reasonable explanations for the biological phenomenon, lead to an enhanced understanding of the gene regulatory network. (hindawi.com)
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies ( Molecular Function , Biological Process , and Cellular Component ), a reference, and an evidence code. (yeastgenome.org)
Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes. (yeastgenome.org)

The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)

first referenced in a paper written (1977) by Daniel Kahneman and Amos Tversky and further explained that the Planning Fallacy is a result of one's inclination to ignore distributional data and instead predict the development of a goal (project) only on the specific elements of one's plan (Scott, 2018). (ostatic.com)

This can be achieved either by cytometric techniques or by nucleic acid-based methods ( 3 ). (iiarjournals.org)
Nonetheless, our results demonstrate that hybrid models integrating ML and artificial neural network (ANN) techniques significantly outperformed traditional methods in predicting gene regulatory relationships. (mtu.edu)

By histological techniques we have characterized the oval cell development, proliferation and differentiation in vivo. (uni-goettingen.de)
In vivo transduction efficiency was studied in normal and injured livers using cell isolation techniques. (duke.edu)

The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

The systems biology approach, combining data-driven modeling and model-driven experiments, provides a systematic and comprehensive perspective on the regulatory roles of miRNAs in gene regulatory networks [ 3 - 5 ]. (hindawi.com)

These data demonstrate the feasibility of using cell-free virus preparations for infecting clinical marrow samples suitable for transplantation, as well as for further analysis of human marrow stem cell dynamics in vitro. (jci.org)
Data from the new study was published recently in Nature Microbiology through an article entitled " Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi . (genengnews.com)
DNA analysis revealed that each harbors a single missense mutation in the AChR alpha-subunit gene, alphaF256L. (ox.ac.uk)
First, state-of-the-art analytical techniques were employed to determine key structural attributes of AC3, including primary and higher-order structures, particle size, empty/full capsid ratios, aggregates, and multi-step thermal degradation pathway analysis. (bvsalud.org)
Until recently, however, functional analysis of the DED fungi was hampered by the lack of protocols for efficient gene knockout and by the unavailability of a full genome sequence. (sisef.it)
Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene internal transcribed spacer-1 (ITS1) was used. (who.int)

Our emerging clinical data demonstrates that these gene-modified T cells are very active in killing tumor cells initially, but they lose their ability to function within a few weeks. (ca.gov)

The specific receptor against cancer cells that will be transferred to the immune cells and stem cells is called NY-ESO-1 T cell receptor (or TCR). (ca.gov)
In this study, the gene-modified immune cells will be given in combination with the gene-modified stem cells. (ca.gov)
Leveraging existing technology and stored specimens, HLA genes and variants of immune response genes (IL-10, TNFA, IL1A, IL12B, and CD80) are to be sequenced and genotyped, respectively, establishing a broader set of Hemophilia Inhibitor Research Study (HIRS) enrollees to evaluate existing inhibitor risk prediction models and to investigate the feasibility of developing better models using curated variants in a diverse patient population. (cdc.gov)

"In the past, the gene for the scissors has been [sic] transferred to the cells, which is dangerous because the cell keeps on producing scissors which can start cutting uncontrollably," said Mikkelsen. (rt.com)
Clinical uses of gene transfer to bone marrow transplants require the establishment of a reproducible method for infecting large numbers of very primitive hematopoietic cells at high efficiency using cell-free retrovirus-containing media. (jci.org)
The gene can be edited while the cell repairs the damage. (genengnews.com)
From the data of the present study we conclude that the cell cycle genes CDC42 and cyclin D1 could be involved in the mechanism of proliferation of the oval cell in the liver regeneration process. (uni-goettingen.de)
Multi-component polymeric system for tumour cell-specific gene delivery using a universal bungarotoxin linker. (ox.ac.uk)

Scholars@Duke publication: Adenovirus-mediated gene transfer to nonparenchymal cells in normal and injured liver. (duke.edu)
The aim of this study was to evaluate gene transfer to nonparenchymal (and parenchymal) cells of the normal and injured rat liver. (duke.edu)

Interestingly, the study found that social media coverage of gene editing has been surprisingly positive in recent years, even as it has reached substantially bigger audiences. (sciencedaily.com)

However, most current studies on exRNA in renal diseases were derived from limited numbers of patients or in vitro data. (biomedcentral.com)

Gene editing and genetically modified organisms (GMOs) have been topics of significant debate in recent years. (sciencedaily.com)
The Bacteria and Archaea are the most genetically diverse superkingdoms of life, and techniques for exploring that diversity are only just becoming widespread. (ox.ac.uk)

Recombinant adeno-associated viruses (rAAVs) are a preferred vector system in clinical gene transfer. (bvsalud.org)

A new study has revealed a positive shift in public sentiment towards one aspect of agricultural biotechnology, showing that gene editing consistently receives higher favorability ratings than GMOs in both social and traditional English-language media. (sciencedaily.com)
A new study from the Alliance for Science, an initiative based at the Boyce Thompson Institute, has revealed a positive shift in public sentiment towards one aspect of agricultural biotechnology, showing that gene editing consistently receives higher favorability ratings than GMOs in both social and traditional English-language media. (sciencedaily.com)
We believe that the scientific community can therefore be cautiously optimistic based on current trends that gene editing will be accepted by the public and be able to achieve its promise of making a substantial contribution to future food security and environmental sustainability worldwide. (sciencedaily.com)
The study, published in the journal GM Crops & Food , revealed a consistent difference between the favorability of gene editing and GMOs, with gene editing enjoying substantially higher favorability ratings than GMOs. (sciencedaily.com)
This could be the result of the relative novelty of gene editing as an issue, which has not -- unlike GMOs -- been the subject of a decades-long campaign of negative publicity by opponents," Lynas explained. (sciencedaily.com)
The research team also noted that the scientific community may have learned from its early deployment of GMOs and made efforts to avoid repeating them as gene editing is used more extensively. (sciencedaily.com)
While the study reveals a positive trend in public acceptance of gene editing, the authors caution that recent indications of sustained downward trends may be a cause for concern. (sciencedaily.com)
The positive coverage of agricultural gene editing in both traditional and social media is encouraging," said Dr. Sheila Ochugboju, Executive Director of the Alliance for Science. (sciencedaily.com)
These findings suggest that the battle for public opinion is not lost, and the scientific community can find hope in the potential acceptance of gene editing technologies. (sciencedaily.com)

We used genomic and epidemiologic data to reconstruct transmission networks of outbreak-related clones. (cdc.gov)

The data provides valuable insights for the scientific community and professionals in science communication. (sciencedaily.com)

Wedbush Securities analyst Liana Moussatos told Reuters that investors may have expected clinical efficacy data. (pharmaphorum.com)

The types of exRNA that have been found are as follows: microRNA (miRNA, miR), small interfering RNA (siRNA), messenger RNA (mRNA), transfer RNA (tRNA), long non-coding RNA (lncRNA), piwi-interacting RNA (piRNAs) and small nucleolar RNA (snoRNA) [ 7 ]. (biomedcentral.com)

The "hit-and-run" technique could be used to modify cells to make them resistant to the HIV infection. (rt.com)
Retroviral gene transfer to primitive normal and leukemic hematopoietic cells using clinically applicable procedures. (jci.org)
Marrow cells enriched greater than or equal to 500-fold for LTC-IC (1-2% pure) by flow cytometry showed gene transfer efficiencies of 27% when infected in a similar fashion over a shorter period (24 h), but in the presence of added soluble IL-3 and G-CSF without stromal feeders, and this increased to 61% when Steel factor was also present during the infection period. (jci.org)
This experience points to the need to have a continuous source of gene-modified cells to maintain the ability to kill cancer cells. (ca.gov)
In this study, we hypothesize that gene-modified stem cells will allow a sustained production of active T cells with antitumor activity. (ca.gov)
Since there is a delay in the appearance of the T cells that come from stem cells to get out of the bone marrow and into the blood, we will give patients both gene-modified T cells for a first wave of antitumor activity and gene-modified stem cells which will provide a bridge until the stem cells have produced more T cells. (ca.gov)
The patient's own white blood cells and stem cells from their blood are modified in the laboratory using genetic techniques to express a specific receptor against cancer cells. (ca.gov)
To date, we have manufactured a batch of the lentiviral vector necessary to transfer the NY-ESO-1 TCR into stem cells and have demonstrated that this vector can gene-modify human stem cells. (ca.gov)
We have demonstrated that when mouse stem cells are gene-modified with this lentiviral vector, the stem cells take up residence in the bone marrow and produce appropriate blood cells. (ca.gov)
A preclinical study is also ongoing in mice to assess the safety of combining the gene-modified T cells and stem cells in mice. (ca.gov)
The vector includes a suicide gene which we have shown can be used to kill cells if necessary. (ca.gov)
A mutagenesis approach was undertaken to improve various aspects of the transposon, including safety and overall efficiency of gene transfer in human cells. (mdc-berlin.de)
Studies of a pckA-lacZ gene fusion indicated that when cells were grown in minimal media with various carbon sources, such as succinate, malate, pyruvate, lactate, or ethanol, under both anaerobic light and aerobic dark conditions, the pckA gene was induced in log phase, irrespective of the carbon source. (asm.org)
Lastly, we evaluated the strategy of knocking out a-syn as a means to protect the cells from transfer of pathology upon grafting. (lu.se)

Next, the network was translated into a detailed mechanistic model, which was characterized and validated with experimental data. (hindawi.com)

In this article, we will present some of these techniques and their application for prioritizing causal loci and developing improved varieties in Breeding 4. (springer.com)
Redundant data is often included in a data warehouse in order to provide users with multiple views of information that present it in logical, easily understood groupings. (ostatic.com)

The main advantage of this technique is that the boldness, aggression, shoaling combination encompasses many well studied, easily quantifiable behaviors in Zebrafish. (jove.com)

And my luck was that I entered brain research at the time, in the late 1960s, when modern neuroscience was born, and in the labora- tory where a new groundbreaking microscopic technique, the Falck-Hillarp monoamine histofluorescence method, had just been developed. (lu.se)

The researchers developed a new CRISPR tool to study which genes are targeted by particular antibiotics, providing clues on how to improve existing drug compounds or develop new ones. (genengnews.com)
This article examines two cohorts aged 25-49 from the 1988 ( n = 7,246) and 2013 ( n = 7,014) Panel Study of Income Dynamics Roster and Transfers Files. (springer.com)
made a mo- people in 98 countries, with a global es- lecular study of cutaneous leishmaniasis Data collection timated incidence of 2 million cases per in northern parts of Afghanistan ( 22 ). (who.int)

Recent isolation and detection techniques also remained controversial. (biomedcentral.com)

Designed for decision support and BI activities, the data warehouse is separated from the day-to-day online transaction processing (OLTP) applications that drive the core business, thereby reducing contention for both operational transactions and analytical queries. (ostatic.com)

To understand the origin of 2,4-D degradation in this diverse collection, the first gene in the 2,4-D pathway, tfdA, was sequenced. (nih.gov)

Anatomy2

Organisms3

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Psychiatry and Psychology1

Phenomena and Processes11

Information Science2

Health Care1

Bacterial2

Differentially expressed3

Sequences5

Plasmid6

Genomes5

Humans1

Vectors5

Proteins2

Regulation7

Expression11

Horizontal2

Phenotypes2

20222

Therapy8

Protein3

Involves3

Conjugation1

Coli2

Characterization2

Efficient Gene Transfer1

20194

Viral1

Enzyme4

Genetic material1

Homologous1

Targets3

Approaches2

Species5

Analyses1

Regulate1

Interactions3

Quantitative4

Microbial1

Adenoviral1

Biological6

Transfection1

20181

Methods2

Vivo2

Therapies1

Regulatory networks1

Analysis6

Clinical data1

Immune3

Cell5

Liver2

Interestingly1

Vitro1

Genetically2

Vector system1

Editing9

Genomic1

Insights1

Efficacy1

MiRNA1

Cells14

Experimental data1

Present2

Combination1

Microscopic1

Study3

Isolation1

Analytical1

Pathway1