dATP and dGTP predominantly accumulates in lymphoid tissue. (medscape.com)
In both adenosine deaminase and purine nucleoside phosphorylase deficiencies, thymocytes are thought to be selectively destroyed because of elevated levels of dATP and dGTP. (medscape.com)
The encoded protein also displays weak activity against dTTP and dATP, but none against dGTP. (nih.gov)
Kullanıma hazır moleküler sınıf dNTP Karışımları kontaminasyon riskini en aza indirmek ve araştırmacılara zamandan tasarruf sağlamak amacıyla dATP,dCTP, dGTP ve dTTP içerecek şekilde tasarlanmıştır. (ardimed.com)
Tüm Meridian dNTP Karışımları, lityum tuzları olarak ultra saf dATP, dCTP, dGTP ve dTTP'nin eşit molarda bir çözeltisi olarak sağlanır. (ardimed.com)
DCTP2
Use α-[ 32 P] dCTP or α-[ 32 P] dGTP for the fill-in reaction. (neb.com)
When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. (addgene.org)
Deoxyguanosine6
Here we show that DNA polymerase (pol) μ insertion of 7,8-dihydro-8'-oxo-dGTP (8-oxodGTP) or deoxyguanosine triphosphate (dGTP) into a model double-strand break DNA repair substrate with template base T results in efficient ligation by DNA ligase. (nih.gov)
The resulting excess of waste molecules and further reactions involving them lead to the buildup of a substance called deoxyguanosine triphosphate (dGTP) to levels that are toxic to lymphocytes. (medlineplus.gov)
One of the most studied modifications of dNTP is 8-hydroxy-2′-deoxyguanosine-triphosphate (8-oxo-dGTP), which is formed when ROS react with deoxyguanosine triphosphate (dGTP). (biomedcentral.com)
A protein called MTH1 dephosphorylates 8-oxo-dGTP to 8-hydroxy-2′-deoxyguanosine-monophosphate (8-oxo-dGMP). (biomedcentral.com)
They focused on the commonly oxidized 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGTP), which may pair with either cytosine or adenine. (nih.gov)
Maki H et al have shown that the product of the mutT gene hydrolyses an oxidised form of guanine nucleotides, 8-oxo-2′-deoxyguanosine 5′-triphosphate (8-oxo-dGTP). (bmj.com)
Polymerase1
In DNA polymerase (Pol) beta, an enzyme mainly involved in DNA repair, the side chain of amino acid aspartate 276 prevents Pol beta from incorporating the oxidatively-induced damaged nucleotide 4,6-Diamino-5-formamidopyrimidine (Fapy-dGTP) into DNA. (nih.gov)
Nucleotides1
1 8-oxo-dGTP incorporated into the genome stably pairs with adenine as well as cytosine in the template strand, accumulation of this oxidised form of guanine nucleotides leads to an increase in base substitution mutations-that is, A:T to G:C and G:C to T:A transversions. (bmj.com)
Diphosphatase1
8-oxo-dGTP diphosphatase (EC 3.6.1.55, MutT, 7,8-dihydro-8-oxoguanine triphosphatase, 8-oxo-dGTPase, 7,8-dihydro-8-oxo-dGTP pyrophosphohydrolase) is an enzyme with systematic name 8-oxo-dGTP diphosphohydrolase. (wikipedia.org)
Mitochondrial1
[ 4 ] The immune deficiency in purine nucleoside phosphorylase deficiency may be the result of inhibited mitochondrial DNA repair due to the accumulation of dGTP in the mitochondria. (medscape.com)
Substrate1
We determined the structure of Gp1.2 by NMR spectroscopy and solved high-resolution cryo-electron microscopy structures of the Dgt-Gp1.2 complex also including either dGTP substrate or GTP coinhibitor bound in the active site. (nih.gov)
Synthesis2
dGTP-templated facile synthesis of luminescent gold nanoclusters (Au NCs) in the presence of cisplatin following PEG coating developed spherical composite NPs. (figshare.com)
dGTP inhibits ribonucleotide reductase, which is needed for synthesis of deoxynucleotides. (medscape.com)
Enzyme1
This enzyme catalyses the following chemical reaction: 8-oxo-dGTP + H2O ⇌ {\displaystyle \rightleftharpoons } 8-oxo-dGMP + diphosphate This enzyme requires Mg2+. (wikipedia.org)
Insertion1
The discovery, made by NIEHS scientists and their collaborators, sheds more light on how Pol beta has evolved to prevent Fapy-dGTP insertion, a process that potentially results in adverse health outcomes, such as cancer. (nih.gov)
Adenine1
The technique revealed that 8-oxo-dGTP is inserted opposite cytosine in one conformation and opposite adenine in another. (nih.gov)
Damage1
Damage to brain cells caused by buildup of dGTP is thought to underlie the neurological problems that occur in some people with purine nucleoside phosphorylase deficiency. (medlineplus.gov)
Potentially1
Nudix proteins act as homeostatic checkpoints at important stages in nucleoside phosphate metabolic pathways, guarding against elevated levels of potentially dangerous intermediates, like 8-oxo-dGTP, which promotes AT-to-CG transversions (Safrany et al. (nih.gov)
Occur1
In purine nucleoside phosphorylase deficiency, similar changes occur, resulting in elevated deoxy-GTP (dGTP) levels. (medscape.com)
Similar1
Because Fapy-dGTP was difficult to isolate, the researchers produced a version that was similar in structure, called Beta-C-Fapy-dGTP. (nih.gov)