Iron-sulfur cluster assembly enzyme ISCU, mitochondrial is a protein that in humans is encoded by the ISCU gene. (wikipedia.org)
It encodes an iron-sulfur (Fe-S) cluster scaffold protein involved in [2Fe-2S] and [4Fe-4S] cluster synthesis and maturation. (wikipedia.org)
ISCU encodes a component of the iron-sulfur (Fe-S) cluster scaffold responsible for the synthesis and maturation of [2Fe-2S] and [4Fe-4S] clusters. (wikipedia.org)
In one process, the [2Fe-2S] cluster transiently assembles on ISCU and is then transferred to GLRX5 in a cysteine desulfurase complex NFS1-LYRM4/ISD11 dependent process. (wikipedia.org)
Using inductively coupled plasma mass spectrometry, UV-visible absorption, EPR, and Mössbauer spectroscopies, we characterized nsp13 as an iron-sulfur (Fe-S) protein that ligates an Fe4S4 cluster in the treble-clef metal-binding site of its zinc-binding domain. (bvsalud.org)
Exposure of the protein to the stable nitroxide TEMPOL oxidizes and degrades the cluster and drastically diminishes unwinding activity. (bvsalud.org)
Along with its most important role as central reductant, T[SH] 2 have also been assumed to regulate the activation of iron-sulfur cluster proteins (Fe/S). Fe/S clusters are versatile cofactors of various proteins and execute a much broader range of essential biological processes viz. (frontiersin.org)
Although, several Fe/S cluster proteins and their roles have been identified in Leishmania, some of the components of how T[SH] 2 is involved in the regulation of Fe/S proteins remains to be explored. (frontiersin.org)
In pursuit of this aim, a systems biology approach was undertaken to get an insight into the overall picture to unravel how T[SH] 2 synthesis and reduction is linked with the regulation of Fe/S cluster proteins and controls the redox homeostasis at a larger scale. (frontiersin.org)
This has also caused an elevated level of free radicals which apparently affected the activation of Fe/S cluster proteins. (frontiersin.org)
Using the IronSulfur cluster biogenesis in S. cerevisiae as a test case we indicate how this procedure can be used to analyze and validate the network model against experimental results. (biomedcentral.com)
Iron-Sulfur Cluster biogenesis) or complex pathways with an unclear reaction and regulation network, (e. g. cell cycle). (biomedcentral.com)
Pathways5
Hence, we suggest that, by designing highly potent and specific inhibitors of TryR enzyme, inhibition of T[SH] 2 reduction and overall inhibition of most of the downstream pathways including Fe/S protein activation reactions, can be accomplished. (frontiersin.org)
Increasing amounts of data that can be mined for information about how proteins in cells assemble as metabolic pathways, signal transduction pathways, and gene circuits, are generated each day. (biomedcentral.com)
We hope to unravel new redox-regulated cellular processes or signaling pathways in poplar and more generally in plants which are controlled by redox reactions such as thiol-disulfide exchanges and understand how the functions of these proteins are controlled at the cellular level by TRX and GRX. (hal.science)
In this context, the intervention of the TRX and glutathione (GSH)/GRX reducing systems in the sulfur signaling/trafficking pathways is examined as both the TRX and GSH/GRX systems might modulate STR function and in particular be central to the formation of hydrogen sulfide, a molecule susceptible to trigger redox signalling cascades in several physiological situations. (hal.science)
Clusters4
It is an iron-sulfur transferase that contains binding sites for [2Fe-2S] and [4Fe-4S] clusters. (wikipedia.org)
Fe-S clusters are cofactors that play a role in the function of a diverse set of enzymes, including those that regulate metabolism, iron homeostasis, and oxidative stress response. (wikipedia.org)
After focusing for some time on the capacity of several plant GRXs to assemble \[2Fe-2S\] centers into homodimers or into heterodimers with BOLA proteins, we extended our research interest to the functional characterization of the associated protein families (NFUs, SUFA/ISCAs, IBA57s, HCF101/INDH) assumed to participate in the transfer of these Fe-S clusters together with GRX and BOLA. (hal.science)
This functional analysis will combine plant genetics and physiology, molecular and structural biology and biochemistry approaches to determine whether these proteins assemble different types of Fe-S clusters and have specific interaction partners and what are the physiological and metabolic consequences of deleting these genes for plant development and physiology. (hal.science)
Oxidative2
This disease is a result of a deficiency of ISCU that corresponds to the deficiency of mitochondrial iron-sulfur proteins and impaired muscle oxidative metabolism. (wikipedia.org)
Redox post-translational modifications of proteins** In addition to performing structure-function analyses of thioredoxin (TRX) and glutaredoxin (GRX) family members which control most of the reversible oxidative modifications of protein cysteinyl residues, we are characterizing proteins of unknown function possessing one or several conserved CXXC motifs known to be particularly suited for disulfide bond formation but also other redox modifications. (hal.science)
Mitochondrial3
As a test case, we reconstruct the topology of the reaction and regulatory network for the mitochondrial ISC biogenesis pathway in S. cerevisiae . (biomedcentral.com)
A putative role for frataxin in directly regulating mitochondrial iron import is discarded from our analysis, which agrees with also published experimental results. (biomedcentral.com)
Here, we review mechanisms of mitochondrial gene expression with a focus on the recent findings in the field of mammalian mtDNA transcription and disease phenotypes caused by defects in proteins involved in this process. (biomed.news)
Genes1
In case you're not sure what is meant by the "genetic code," it refers to how the sequence of bases in DNA (there are four such bases) are translated into amino acids, the constituents of proteins and the products of most genes. (whyevolutionistrue.com)
Synthesis1
Cysteine, a thiol-containing amino acid, is crucial for the synthesis of sulfur-containing biomolecules that control multiple essential cellular activities. (biomed.news)
Heme6
Heme oxygenases (HOs) act on heme degradation to produce carbon monoxide (CO), free iron, ferritin, and biliverdin. (encyclopedia.pub)
HOs are the key-limiting enzymes in heme degradation leading to carbon monoxide (CO), ferrous iron, and biliverdin products. (encyclopedia.pub)
HMOX2 is constitutively expressed to encode a 36 kDa HO-2 protein, mainly functioning to maintain the basal heme metabolism and may also play a role in inflammatory responses [ 4 ] . (encyclopedia.pub)
Heme is degraded by heme oxygenases (HOs), generating biliverdin, carbon monoxide, and ferrous iron (Fe 2+ ). (encyclopedia.pub)
However, activation of heme oxygenase-1 (HO-1) can upregulate ferritin expression, which binds to ferrous iron and detoxifies its pro-oxidant effect. (encyclopedia.pub)
The pH‐Induced Selectivity Between Cysteine or Histidine Coordinated Heme in an Artificial α‐Helical Metalloprotein. (cnrs.fr)
Enzyme1
protein_coding" "AAC74888","manY","Escherichia coli","mannose-specific enzyme IIC component of PTS [Ensembl]. (ntu.edu.sg)
Redox1
I am a biochemist interested in basic plant science processes with a particular research focus on structure-function analysis of oxidoreductases and on redox post-translational modifications of proteins. (hal.science)
Amino1
Stop" refers to stop codons: when the process of protein-making in the ribosomes encounters this codon, the translation is stopped and the string of amino acids ends. (whyevolutionistrue.com)
Roles1
For our research projects, we employ biochemical, biophysical, spectroscopic and structural biology approaches with *Populus trichocarpa* and *Arabidopsis thaliana* as model organisms to explore (i) the roles of the antioxidant/detoxification systems in the physiology of plants subject to environmental constraints and (ii) the maturation and roles of iron-sulfur proteins in plant organelles. (hal.science)
Kinetic1
The more than likely absence of known mechanistic and kinetic data for each of the individual proteins in a novel pathway hinders the process of translating network topology into a mathematical model. (biomedcentral.com)
Reductase2
Multiple membrane-bound heterodisulfide reductase (DsrMK) could promote both energy-conserving and non-energy-conserving menaquinol oxidation. (hindawi.com)
Datasets available for such tasks include the primary literature, large scale micro array experiments, whole genome two hybrid screenings, full genome sequences, and the patterns of conserved/non-conserved homologues and orthologues in them. (biomedcentral.com)
Catalyze1
The same questions and strategies apply to sulfurtransferases, enzymes that catalyze the transfer of a sulfur atom from sulfur donors to nucleophilic sulfur acceptors (trans-persulfidation) by forming themselves persulfides. (hal.science)
Unknown function1
Protein of unknown function (DUF520) [Interproscan]. (ntu.edu.sg)
Complex2
ISCU has been shown to have 235 binary protein-protein interactions including 79 co-complex interactions. (wikipedia.org)
Halide, 2OG, and (lastly) O2 all coordinate directly to the cofactor to initiate its conversion to a cis-halo-oxo-iron(IV) (haloferryl) complex, which abstracts hydrogen (Hâ ¢) from the non-coordinating prime substrate to enable radicaloid carbon-halogen coupling. (bvsalud.org)
Cell2
events have receptors stimulated with students( GAGs), unregulated chains docking of a inactive actin, all of an been protein respiration cell a considerable subunits. (evakoch.com)
This activates signaling to the actomyosin cortex via nuclear envelope stretch-sensitive proteins, up-regulating cell contractility. (regenerativemedicine.net)
Process2
For example, the involvement of proteins X, Y and Z in a process does not elucidate if X catalyzes a reaction that produces a substrate for another reaction catalyzed by Z or by Y, or if X modulates Y or Z activity. (biomedcentral.com)
To achieve such a goal, strategies that combine the different theoretical and computational methods to identify proteins and generate a set of plausible alternative network topologies for the process of interest are needed. (biomedcentral.com)
Control1
Accepted August 23, 2012 ABSTRACT The catabolite control protein CcpA is a pleiotropic regulator that mediates the global transcriptional response to rapidly catabolizable carbohydrates, like glucose in Gram-positive bacteria. (studyres.com)
System2
Transcription of mtDNA is not only essential for the biogenesis of the OXPHOS system, but also generates RNA primers necessary to initiate mtDNA replication. (biomed.news)
protein system, it would be very unlikely that all modern species would have the same code. (whyevolutionistrue.com)
Addition1
Here, we found that nsp13 ligates iron, in addition to zinc, when purified anoxically. (bvsalud.org)
Family2
ISCU, the protein encoded by this gene, is a member of the NifU family. (wikipedia.org)
Predictions regarding how proteins act in ISC biogenesis are validated by comparison with published experimental results. (biomedcentral.com)
Host1
The spectrum of diseases caused by C. difficile is highly variable and depends not only on host factors but also, for the severe forms, on the level of toxin production (4). (studyres.com)