• 2002
  • Garrido JL, Godoy JA, Alvarez A, Bronfman M, Inestrosa NC (2002) Protein kinase C inhibits amyloid beta peptide neurotoxicity by acting on members of the Wnt pathway. (springer.com)
  • Inhibition
  • Inhibition of GSK-3β either by small molecules (SB216763 or SB415286) or by ectopic expression of kinase-inactive GSK-3β before irradiation significantly attenuated radiation-induced apoptosis in hippocampal neurons. (aacrjournals.org)
  • Tubulin-dependent suppression of ΔΨ m is further potentiated by protein kinase A activation and glycogen synthase kinase-3β inhibition. (aspetjournals.org)
  • OBJECTIVE: The role of diacylglycerol in the phosphatidylinositol-signaling pathway is to activate protein kinase C. In the myometrium, protein kinase C activation leads to inhibition of phasic contractions. (biomedsearch.com)
  • RESULTS: In myometrium from nonpregnant animals, inhibition of diacylglycerol lipase with RHC80267 had little effect on oxytocin-stimulated contractile activity, whereas inhibition of diacylglycerol kinase with R59022, although producing an increase in contractile frequency, markedly suppressed total oxytocin-stimulated contractile activity. (biomedsearch.com)
  • CONCLUSIONS: These studies have demonstrated that prevention of diacylglycerol degradation, especially in response to inhibition of myometrial diacylglycerol kinase, results in the paradoxic oxytocin-mediated suppression of total myometrial contractile activity. (biomedsearch.com)
  • These observations support the hypothesis that, when its catabolism is prevented, diacylglycerol produced in response to stimulation of the phosphatidylinositol-signaling pathway by oxytocin becomes available for protein kinase C activation, resulting in inhibition of myometrial contractile activity. (biomedsearch.com)
  • binds
  • When AVP binds with V2R , via the G-protein , it stimulates the enzyme , adenylyl cyclase (AdC). (ndif.org)
  • isoforms
  • The high '2-Kase' activity of PFKFB3 is also due to the lack of a specific Ser that is phosphorylated in the other PFKFB isoforms to decrease kinase activity. (wikipedia.org)
  • pathway
  • The Wnt proteins are palmitoylated secreted glycoproteins that activate the Wnt signaling pathway. (aacrjournals.org)
  • Frizzled receptors, G protein-coupled receptors, are the major proteins that bind to Wnt proteins on the plasma membrane and trigger Wnt pathway signaling, which leads to activation of T-cell factor (TCF)/lymphoid enhancer factor (LEF) transcription. (aacrjournals.org)
  • The Wnt signaling pathway is partly regulated by Wnt antagonists, including members of the Dickkopf and secreted Frizzled-related protein (sFRP) families, and Wnt inhibitory factor 1 ( 6 , 7 ). (aacrjournals.org)
  • sFRPs are modulators of the Wnt signaling pathway, interact with Wnt proteins, and prevent Frizzled receptors from binding to Wnt proteins, thereby downregulating Wnt signaling ( 8 ). (aacrjournals.org)
  • These studies are designed to determine why stimulation of the phosphatidylinositol-signaling pathway caused by oxytocin does not cause a paradoxical suppression of contractions through diacylglycerol production and protein kinase C activation. (biomedsearch.com)
  • The signaling pathway of merlin is proposed to include several salient cell growth controlling molecules, including eIF3c, CD44, protein kinase A, and p21 activated kinases. (wikipedia.org)
  • intracellular
  • Central to its antidiuretic action in mammals is the redistribution of the water channel aquaporin 2 ( AQP2 ) from intracellular vesicles to the apical membrane of kidney epithelial cells , an event initiated by an increase in cAMP and activation of protein kinase A . The subsequent steps of the signaling cascade are not known. (ndif.org)
  • vitro
  • Self-assembly in vitro of the 68,000 molecular weight component of the mammalian neurofilament triplet proteins into intermediate-sized filaments. (springer.com)
  • In vitro contraction studies were performed with cumulative additions of oxytocin (8-64 nmol/L) with and without R59022 (a diacylglycerol kinase inhibitor) or RHC80267 (a diacylglycerol lipase inhibitor). (biomedsearch.com)
  • isoform
  • The inducible protein isoform, iPFK2, is named as such because its expression has been shown to be induced by hypoxic conditions. (wikipedia.org)
  • calcium
  • When activated by the second messengers such as cAMP and calcium ions, enzymes such as PKA and MAP kinase can translocate to the nucleus and phosphorylate CREB to initiate transcription of target genes. (wikipedia.org)
  • putative
  • The sFRPs are a family of soluble glycoproteins, which contain cysteine-rich domains homologous to the putative Wnt-binding sites of Frizzled proteins. (aacrjournals.org)
  • PRKAR2A
  • RUNX1T1 has been shown to interact with: CBFA2T2, CBFA2T3, Calcitriol receptor GFI1, Nuclear receptor co-repressor 1, Nuclear receptor co-repressor 2, PRKAR2A, and Zinc finger and BTB domain-containing protein 16. (wikipedia.org)
  • test the hypothesis
  • Specifically, these studies were performed to test the hypothesis that diacylglycerol catabolism is significant in myometrial tissue, thereby precluding its availability for the activation of protein kinase C. STUDY DESIGN: For these studies, uterine tissue was obtained from Sprague-Dawley rats both nonpregnant and with timed gestations. (biomedsearch.com)
  • genes
  • PKA inhibitors can block the development of long-lasting LTP, and this is accompanied by a reduction in the transcription of genes modulated by the CREB protein. (wikipedia.org)
  • This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids Three alternatively spliced transcript variants encoding the same protein have been observed. (wikipedia.org)
  • neuronal
  • Here, we report that cannabidiol inhibits hyperphosphorylation of tau protein in Aβ-stimulated PC12 neuronal cells, which is one of the most representative hallmarks in AD. (springer.com)
  • Here we show that glycogen synthase kinase 3β (GSK-3β) is required for radiation-induced hippocampal neuronal apoptosis and subsequent neurocognitive decline. (aacrjournals.org)
  • At the neuronal level, cells with higher levels of excitability (for example lower slow afterhyperpolarization) are more likely to be recruited into a memory trace, and substantial evidence exists implicating the cellular transcription factor CREB (cyclic AMP responsive element-binding protein) in this process. (wikipedia.org)