• cell
  • Sensitivity may be increased in a single image by dwelling over the cell for a longer period of time, but this can cause excessive photobleaching outside of the plane of focus, hindering 3D imaging. (pubmedcentralcanada.ca)
  • Recovery of a bacterial sub‐population from sewage using immunoflourescent flow cytometry and cell sorting. (currentprotocols.com)
  • Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. (wikipedia.org)
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (wikipedia.org)
  • In a similar fashion, cytometry is also used in cell biology research and in medical diagnostics to characterize cells in a wide range of applications associated with diseases such as cancer and AIDS. (wikipedia.org)
  • This has led to the commercial availability of automated image cytometers, ranging from simple cell counters to sophisticated high-content screening systems. (wikipedia.org)
  • The early history of cytometry is closely associated with the development of the blood cell counting. (wikipedia.org)
  • Vasomotor function of the endothelium, mediated mainly by nitric oxide (NO) and widely accepted as a measure of generalized endothelial cell function, is readily determined by flow mediated dilation (FMD). (hindawi.com)
  • When no cell is in the gap, electricity flows unabated, but when a cell is sucked into the gap the current is resisted. (wikipedia.org)
  • In recent years, acoustic tweezers has found many important applications in the area of manipulating sub-millimeter objects, such as flow cytometry, cell separation, cell trapping, single-cell manipulation, and nanomaterial manipulation. (wikipedia.org)
  • populations
  • The current protocol takes advantage of magnetic sorting of surface-labeled yeast mother cells to obtain large enough populations of aging mother cells to quantify rare mutations through phenotypic selections. (jove.com)
  • cells
  • The technique is a good fit for clinical applications by providing a convenient means for imaging and analyzing cells directly in bodily fluids. (pubmedcentralcanada.ca)
  • A touch-screen user interface based on a tablet-PC is developed to reconstruct the holographic shadows captured by the image sensor chip and use a support vector machine (SVM) model to automatically classify live and dead cells in a yeast sample stained with methylene blue. (rsc.org)
  • Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count. (wikipedia.org)
  • Conventional flow and image cytometers have the disadvantage of not being able to observe cells over time. (wikipedia.org)
  • The rapid decrease in the cost of digitally storing and processing video information has led to the development of image cytometers which monitor cultured cells using time-lapse video recordings. (wikipedia.org)
  • Cells may be live or fixed, depending on the application, and individual cells can often be physically sorted using a cytometer. (isac-net.org)
  • Flow cytometry is generally used for analyzing individual cells in a suspension, and is common in immunology and haematopathology. (isac-net.org)
  • technology
  • The FISH application will be further enhanced by the integration of extended depth of field imaging technology with the current optical system. (pubmedcentralcanada.ca)
  • At the University of Alberta in Edmonton, Canada, Xuantao Su and colleagues are looking into optofluidic technology to aid in the development of the next generation of portable cytometers for point-of-care and other applications. (photonics.com)
  • More recently, the new technology of mass cytometry replaces fluorophores with rare-earth elements detected by time of flight mass spectrometry, achieving the ability to measure the expression of 34 or more markers. (wikipedia.org)
  • mechanical
  • We sought to define the time course of endothelial function recovery using flow-mediated dilation (FMD), after ischaemia-reperfusion (IR) and mechanical injury in patients and healthy volunteers. (hindawi.com)
  • device
  • The first impedance-based flow cytometry device, using the Coulter principle , was disclosed in U.S. Patent 2,656,508, issued in 1953, to Wallace H. Coulter . (wikipedia.org)