Cultures cells cultured. Medical search. Frequent questions

Cell cultured meat, also known as cultivated, cultured, or lab-grown meat, is a product of cellular agriculture , that uses tissue samples from living animals to grow sustainable, humane meat in a bioreactor. (moleculardevices.com)
Bioreactor flasks are specialized vessels used to culture cells at a large scale, making them a valuable tool for the production of mAbs. (coleparmer.com)
While hybridoma cells can be cultured in standard culture vessels, bioreactor flasks provide several advantages when scaling up the production process. (coleparmer.com)
Bioreactor flasks have a large surface area and volume, allowing for increased cell density and greater antibody production. (coleparmer.com)

Cell-cultured meat pioneer UPSIDE Foods (formerly Memphis Meats) has brought in high-flying PepsiCo exec Amy Chen as its chief operating officer as it prepares to launch its first consumer product - UPSIDE chicken - later this year, pending regulatory. (foodnavigator-usa.com)

Traditional random cultures allow limited access to subcellular compartments (axons, dendrites, synapses) due to extensive and haphazard growth of neurons. (sbir.gov)
Our long-term goal is to provide robust, user-friendly, and cost effective culture platforms that can optically, fluidically, and biochemically access neurons and their subcellular compartments. (sbir.gov)
Such improvements include ensuring greater uniformity of the device, reducing end-user assembly procedures, and enhancing viability for the long-term culturing period that is often needed for neurons. (sbir.gov)
The significance of the proposed work is to improve research scientists' ability to visualize, manipulate, and measure cultured neurons leading to greater understanding of the underlying causes of neurological diseases. (sbir.gov)
Double-staining studies with specific cell markers to distinguish astrocytes, neurons and oligodendrocytes were performed. (lu.se)
COMT immunoreactivity colocalized with a specific oligodendrocyte marker galactocerebroside in cells displaying oligodendrocyte morphology, flat cells displaying type-1 astrocyte morphology and glial fibrillary acidic protein, in branched cells displaying type-2 astrocyte morphology and in cell bodies of neurons, the processes of which displayed neurofilament immunoreactivity. (lu.se)
The results suggest that COMT is synthesized by cultured astrocytes, oligodendrocytes and neurons. (lu.se)

Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). (wikipedia.org)
Advancements in cell culture technology enable scientists to use animal cells obtained from livestock, poultry, or seafood to produce food products. (usda.gov)
There is a long history of scientific advances in biology, biochemistry, and engineering that have led to the innovations enabling the growth of animal cells outside of the animal itself, in a controlled environment, for food. (usda.gov)
Regulatory oversight of human foods comprised of or containing cultured animal cells depends on the animal species used as the original source of cultured cells, and it is based on the agencies' existing jurisdiction over products. (usda.gov)
Because cell-cultured meat is made with animal cells, many processes for developing and scaling a cell-cultured meat product will mirror those in biologics production, specifically cell line development . (moleculardevices.com)
Animal cells are usually engineered to produce different biologics, including growth factors, hormones, and antibodies that are commonly used as therapies today. (moleculardevices.com)

FSIS and FDA have held public meetings to better understand the science of animal cell culture technology, potential hazards, labeling considerations, and to listen to consumer concerns. (usda.gov)
On March 7, 2019, FDA and FSIS agreed to establish a joint regulatory framework for human foods made from cultured cells of livestock and poultry to help ensure that any such products brought to market are safe, unadulterated, and truthfully labeled. (usda.gov)
Regulatory jurisdiction transitions from FDA to FSIS during the harvesting stage of the cell-culturing process. (usda.gov)

The USDA has issued an advance notice of proposed rulemaking (ANPR) to solicit comments on how to label cell-cultured meat and poultry as startups in the space edge closer to commercialization, but says it will review labels submitted before the rulemaking. (foodnavigator-usa.com)

The current objective was to develop a technique for selective isolation and culture of endothelial cells from human SC. (nih.gov)
Adherent cells such as fibroblasts, epithelial cells, and endothelial cells need a proper surface to attach to, and improper attachment can lead to cell death. (coleparmer.com)

Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture. (wikipedia.org)
He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
The entire process of going from animal tissues to cultured meat products described above is time-consuming, labor-intensive, and prone to error if done in a manual fashion. (moleculardevices.com)

Traditional cell culture was developed on simple, nonporous two-dimensional (2D) surfaces, which facilitated the expansion of this vital technique across the life sciences. (sigmaaldrich.com)
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. (sigmaaldrich.com)
After the cells have multiplied many times over into billions or trillions of cells, additional factors (e.g., protein growth factors, new surfaces for cell attachment, additional nutrients) are added to the controlled environment to enable the cells to differentiate into various cell types and assume characteristics of muscle, fat, or connective tissue cells. (usda.gov)
The right culture vessel is crucial here, and different cell types may require different surfaces, such as plastic, glass, or coated surfaces. (coleparmer.com)
Role of rhBMP-2 and rhBMP-7 in the metabolism and differentiation of osteoblast-like cells cultured on chemically modified titanium surfaces. (bvsalud.org)
This study analyzed the role of recombinant human bone morphogenetic protein 2 (rhBMP-2) and recombinant human bone morphogenetic protein 7 (rhBMP-7) in the adhesion and differentiation of rat osteoblast -like (osteo-1) cells cultured on chemically modified titanium surfaces. (bvsalud.org)
Osteo-1 cells were cultured on chemically modified (modified sandblasted and acid -etched) titanium surfaces in 3 different types of medium control, medium supplemented with 20 ng/mL rhBMP-2, and medium supplemented with 20 ng/mL rhBMP-7. (bvsalud.org)
Within the limitations of the study, we conclude that the presence of rhBMP-2 or rhBMP-7 did not influence cell adhesion to chemically modified titanium surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells cultured on this type of surface. (bvsalud.org)

Producing highly productive cell lines and optimized cell culture conditions requires an understanding of the contributions of many key variables: cell culture media, cell growth rates, cell viability, protein expression levels, and other quality attributes including size, aggregation status, glycosylation and stability. (beckman.com)
Culturing suspension cells can be tricky because they need specialized culture vessels and techniques to maintain their viability and growth. (coleparmer.com)

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)

Cell line development workflows consist of a multistep process involving single-cell cloning, formation and isolation of these discrete colonies, and the phenotypic characterization of these colonies. (moleculardevices.com)
Cannulation of SC with gelatin-coated suture material is an effective method for the isolation of human SC cells and provides a cellular model to study the potential role of SC cells in aqueous humor outflow function. (nih.gov)

This research is innovative because we seek to shift current research paradigms through the development of novel cell-based tools to isolate synapses that maintain intact cell morphology in the absence of somata or glia. (sbir.gov)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. (wikipedia.org)
In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Ross Granville Harrison, working at Johns Hopkins Medical School and then at Yale University, published results of his experiments from 1907 to 1910, establishing the methodology of tissue culture. (wikipedia.org)
In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. (wikipedia.org)
For adherent cells, use tissue culture flasks or tissue culture dishes with the right coating to promote cell attachment and growth. (coleparmer.com)
C trachomatis grows well in a variety of cell lines (eg, McCoy and HeLa cells) that can be maintained in tissue culture. (medscape.com)
Incubation in tissue culture is 40-72 hours, depending on the cell type and specific biovar. (medscape.com)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. (wikipedia.org)
Adherent cells can quickly reach confluency, meaning the entire surface of the culture vessel is covered with cells, which can lead to nutrient depletion and cell death. (coleparmer.com)
Lastly, detaching adherent cells from the surface for passaging or analysis can be tough and can lead to cell damage. (coleparmer.com)

Cultured meat (CM) is an emerging technology, an alternative to the traditional farming industry, and crucial to supply the increasing meat demand while being more environmentally and animal friendly. (moleculardevices.com)
Is cell-cultured meat approved? (moleculardevices.com)
Cultivated meat, cell-cultured meat, cell-based meat? (foodnavigator-usa.com)
Cell-based disruption: How many factories, and at what capacity, are required to supply 10% of the meat market? (foodnavigator-usa.com)
The world's largest food company has confirmed it is eyeing the cultured meat sector after a leaked report revealed a tie-up with Israeli cell-based start-up Future Meat Technologies. (foodnavigator-usa.com)
With Aleph Farm's 2022 initial market launch just on the horizon, the trailblazing food-tech company responsible for series of firsts in the nascent cell-cultured meat category, has secured $105m in series B funding to scale-up manufacturing and expand. (foodnavigator-usa.com)

This study confirms the absence of overt viruses in renal biopsy cultures. (karger.com)

Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
Screening of monolayer and spheroid cultures was found to identify different sets of hit compounds. (researchgate.net)
Spheroids of EMT-6 mammary tumor cells were markedly more resistant to different exposure doses of Adriamycin (ADR) than monolayer cells in exponential or plateau growth phases. (researchgate.net)
This resistance was not due to differences in the cell cycle state of these inner cells since separate experiments showed that both exponential and plateau phase monolayer cells were about equally sensitive when the surviving fraction was plotted vs absorbed drug (D0, = 0.04 μ/106 cells). (researchgate.net)

Schlemm's canal cell isolates reacted with antibodies specific for CD44 (hyaluron receptor), CD54 (intercellular adhesion molecule-1, ICAM-1), tissue-type plasminogen activator, and TM-inducible glucocorticoid-responsive protein-myocilin (TIGR-MYOC). (nih.gov)

Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)

Some cells from the sample are selected, screened, and grown to make a "bank" of cells to store for later use. (usda.gov)

Under this agreement, FDA will oversee the collection, growth, and the differentiation of living cells into various cell types, such as proteins and fats. (usda.gov)
Western blots detected both soluble 24 kDa and membrane-bound 28-kDa COMT proteins in neuronal and astrocyte cultures. (lu.se)

Unlike TM cells, however, TIGR-MYOC protein was not induced in SC cells after long-term dexamethasone treatment. (nih.gov)
Monitoring and maintaining robust cell health and protein quality are critical to scale from the lab to commercialization. (beckman.com)

Cytology is used mainly for diagnosing infant inclusion conjunctivitis and ocular trachoma through the demonstration of intracytoplasmic C trachomatis inclusions in HeLa cells (ie, continuously cultured carcinoma cell line used for tissue cultures). (medscape.com)

When the final cell culture process is established, companies move forward with the commercialization process which includes process characterization, scale-up, technology transfer and manufacturing process validation. (beckman.com)

Spheroid culture is often favored for high-throughput compound screening in drug development and toxicology, where spheroids present more biologically-relevant models than 2D cultures. (sigmaaldrich.com)
Spheroid culture can be achieved in diverse environments including low-attachment microplates, bioreactors, and microfluidics culture systems. (sigmaaldrich.com)
By using a selective disaggregation technique after intact spheroids had been exposed to the drug it was possible to show directly that the inner spheroid cells were most resistant (D0 = 0.25 μ/106 cells). (researchgate.net)

Because of its high specificity (100%) and sensitivity, cell culture is the only test that should be used to establish the presence or absence of infections in cases with legal implications, such as those involving rape or sexual abuse. (medscape.com)

These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. (wikipedia.org)
Of the 20 pairs of eyes that were cannulated, primary cells were obtained from 13. (nih.gov)
What are the challenges of culturing primary cells compared to established cell lines? (coleparmer.com)
Culturing primary cells presents unique challenges compared to established cell lines. (coleparmer.com)
Primary cells have a limited lifespan and demand careful handling and specialized conditions to maintain their original properties. (coleparmer.com)

3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (sigmaaldrich.com)
Scientists typically start with a sample of cells from the tissue of an animal or fish, a process that typically does not permanently harm or kill the animal. (usda.gov)
Cell culture process development begins with cell line generation which typically consists of a common host cell such as HEK 293 or CHO cells, an expression or infection system and selection, followed by process and media optimization in small scale systems such as 96-well plates or bench-scale bioreactors. (beckman.com)

This gelatin coating protocol for cell culture applications offers information regarding types of gelatin, surface coverage concentration, and tips for optimization. (sigmaaldrich.com)
During the scale-up process, it is important to maintain productivity and genetic stability, and robustness of the cells, which requires optimization of culture conditions. (moleculardevices.com)

Both scaffolded and scaffold-free systems enable interaction in all directions with substrate, other cells, and extracellular factors. (sigmaaldrich.com)

For example, after 1 hr exposures to 0.5 μ/ml, surviving fractions determined by colony formation assay were approximately 0.3 for cells from spheroids treated intact and 0.001 for single exponential phase cells. (researchgate.net)

Once the cells have differentiated into the desired type, the cellular material can be harvested from the controlled environment and prepared using conventional food processing and packaging methods. (usda.gov)
Depending on the type of cells you're working with, different challenges can arise. (coleparmer.com)

Here, we highlight various applications and research methods using our CloneSelect Imager and Single-Cell Printer, ImageXpress Cellular Imaging System, and SpectraMax® microplate reader to help scale your cell line development workflow. (moleculardevices.com)

To make food, a small number of cells are taken from the cell bank and placed in a tightly controlled and monitored environment (e.g., a very large, sealed vessel) that supports growth and cellular multiplication by supplying appropriate nutrients and other factors. (usda.gov)

The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
Molecular Devices has a history of success in scaling biologics for regulatory approval and commercialization with life science groups worldwide, and can leverage that expertise with food manufacturers growing animal cell lines in new industries. (moleculardevices.com)

This vaccine was made possible by the cell culture research of John Franklin Enders, Thomas Huckle Weller, and Frederick Chapman Robbins, who were awarded a Nobel Prize for their discovery of a method of growing the virus in monkey kidney cell cultures. (wikipedia.org)
COMT immunoreactivity was studied in primary astrocytic cultures from newborn rat cerebral cortex, and in neuronal cultures from rat brain from 18-day-old rat embryos using antisera against rat recombinant COMT made in guinea pig. (lu.se)

Thus, other factors related to the metabolic state of the cells, the microenvironment, or the formation of different drug products must account for the observed resistance. (researchgate.net)

The injectable polio vaccine developed by Jonas Salk was one of the first products mass-produced using cell culture techniques. (wikipedia.org)

Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology. (wikipedia.org)

What are the different cell types? (coleparmer.com)
The aim of this work was to study its localization and molecular forms in primary cultures, where cell types can be easily distinguished with specific markers. (lu.se)

Monoclonality verification is crucial from a quality and regulatory perspective to ensure that the desired characteristics from the colony of cells originate from a single cell. (moleculardevices.com)

Schlemm's canal cells endocytosed low-density lipoprotein and acetylated low-density lipoprotein, and in the presence of Matrigel organized into multicellular tubelike structures. (nih.gov)
We used colon carcinoma multicellular spheroids containing hypoxic and quiescent cells in core areas for this purpose. (researchgate.net)

They also can control environmental conditions, such as temperature, pH, and oxygen levels, to optimize cell growth and antibody production. (coleparmer.com)

Our next aim focuses on the development of novel cell-based tools to study synapses, the cornerstone of neuroscience. (sbir.gov)

Hydrogela are the most widely used systems for 3D cell culture. (sigmaaldrich.com)
DESCRIPTION (provided by applicant): Neuron-cell culture is widely used for studies in basic research, drug discovery, and toxicity testing. (sbir.gov)

Viral culture is also related, with cells as hosts for the viruses. (wikipedia.org)
Growing viruses in cell cultures allowed preparation of purified viruses for the manufacture of vaccines. (wikipedia.org)
This study is concerned with the detectionof overt and latent viruses in explant cultures of renal biopsies from 72 patients with various forms of renal disease. (karger.com)

After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
They are usually much more sensitive to culture conditions. (coleparmer.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph. (wikipedia.org)
These cells support the growth and maintenance of other cells in culture. (coleparmer.com)
Feeder cells can provide essential growth factors, nutrients, and signaling molecules to support the growth of cells that are difficult to culture on their own. (coleparmer.com)
And for feeder cells, use flasks or dishes with a proper surface and growth media to support their growth and function. (coleparmer.com)

Examples include blood cells and some cancer cells. (coleparmer.com)

Cell culture has contributed to the development of vaccines for many diseases. (wikipedia.org)

The aim of this study was to examine the potential usefulness of 3-D cultured tumour cells for anticancer drug screening. (researchgate.net)

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. (sigmaaldrich.com)
These cells are free-flowing which means they don't require a surface to grow on. (coleparmer.com)

Cell culture is also preferred for rectal specimens because nonculture test results are difficult to interpret in the presence of stool organisms. (medscape.com)

One big challenge with suspension cells is aggregation, which means your cells clump together and make it difficult to maintain single-cell suspensions. (coleparmer.com)
The right lab equipment and supplies can make all the difference to the success of your cell culture. (coleparmer.com)

To prevent this, it's important to create single-cell suspensions by gently pipetting or using specialized culture vessels. (coleparmer.com)

Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
His original idea, presented in 1902, was called totipotentiality: "Theoretically all plant cells are able to give rise to a complete plant. (wikipedia.org)
Cultures of the second patient developed multinucleated and vacuolated cells and these changes reappeared after passage with supernatant of the original culture. (karger.com)

Anatomy41

Organisms35

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Chemicals and Drugs67

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Phenomena and Processes78

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