• SERUM
  • We have developed a reliable protocol for the serum-free dissociation and culture of spiral ganglion neurons from adult mice, an important animal model for patients with post-lingual hearing loss. (illinois.edu)
  • Because of the difficulty that advanced method of fish cell culture such as static-suspension, non-serum, and extracellular matrix-dependent culture method, we examined the characteristics of mainly chinook salmon embryo cells (CHSE) and its derivatives. (nii.ac.jp)
  • CHSE-sp cells, which cells completely adapted in suspension-stirred culture and the original of the aim, were selected and developed, and sublines of CHSE-sp were established as CHSE-ss (SS) cells for static-suspension and CHSE-ns (NS) cells for non-serum culture. (nii.ac.jp)
  • However, some claim they are not true alternatives because simulations use data from prior animal experiments and cell cultures often require animal derived products, such as serum or cells. (wikipedia.org)
  • Although exact figures are difficult to obtain, some have estimated that one million fetal cows are sacrificed each year to obtain the world's supply of fetal bovine serum, used to grow cultured cells. (wikipedia.org)
  • 1997
  • 1997) as long as there is a predefined protocol for sample collection and culturing and the analysis and interpretation of results are used as a basis for determining whether there is a need for intervention and which specific actions should be taken (Sehulster et al . (scielo.org.za)
  • 1997: Geometry Civilized: History, Culture, Technique. (wikipedia.org)
  • substrate
  • Publications] Kaneko Y., M.Igarashi, M.Iwashita, K.Suzuki, H.Kojima, S.Kimura and M.Hasobe: 'Effects of fish and calf collagens as culture substrate in the adhesion and spreading process of established fish cells' In Vitro Cell. (nii.ac.jp)
  • Articles
  • In articles such as "Nouvelles inventions: l'interchangeabilité et le génie national" in Culture Technique and "Influence and Intercultural Exchange: the Case of Engineering Schools and Civil Engineering Works in the Nineteenth Century" in History and Technology, Dreicer explores invention as a process of exchange between cultures while emphasizing the thinking behind the history of architecture and building. (wikipedia.org)
  • allow
  • Amy YH, Yi-Chung T, Xianggui Qu, Lalit RP, Kenneth JP, Shuichi T (2012) 384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids. (springer.com)
  • These populations were to be co-cultured to allow filopodial contact to be established between them, after which the contact-receiving cells were to be isolated. (chalmers.se)
  • routine
  • We acknowledge the help of Arina Basyirah Ismail for routine cell sub-culture. (springer.com)
  • Routine, non-targeted sampling and culture of environmental samples collected from healthcare environments is not recommended by the Centers for Disease Control and Prevention (CDC) and other authorities (Sehulster et al . (scielo.org.za)
  • toxic effects
  • Thawing - should occur quickly and cells diluted in pre-warmed culture medium to prevent any toxic effects of cryoprotectants in super-zero temperatures. (edu.au)
  • Liquid
  • The biophysical properties of the microtissues cultured using scaffold based microencapsulation by flicking and scaffoldless liquid crystal (LC) based techniques were characterized. (springer.com)
  • Fe-oxidizers have also successfully been cultivated in liquid culture with FeCl2 as the Fe source. (wikipedia.org)
  • model
  • In 1953 Tamiya, working with other Japanese scientists, developed important techniques for the synchronous culture of the green algae Chlorella, a model organism used by German Nobel Laureate Otto Heinrich Warburg whom Tamiya greatly admired. (wikipedia.org)
  • EpiDerm from Mattek and EpiSkin and SkinEthic RHE model are derived from human skin cells which have been cultured to produce a model of human skin. (wikipedia.org)
  • growth
  • In both techniques, cells remodeled into microtissues via different growth phases and showed good integrity of cells in field-emission scanning microscopy (FE-SEM). (springer.com)
  • Cell growth, structural organization, morphology and surface structures for 3D microtissues cultured using both techniques appeared to be different and might be suitable for different applications. (springer.com)
  • NS cells secreted the cell adhesion and growth substances in the culture medium. (nii.ac.jp)
  • disease
  • Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely characterizes the disease state have been undertaken by a number of laboratories around the world. (mdpi.com)
  • Tube
  • In "Building Bridges and Boundaries: The Lattice and the Tube, 1820-1860" in Technology and Culture he analyzes the relationship between the construction of engineering infrastructure and national identity. (wikipedia.org)
  • The headspace of the culture tube is then purged with air or a low concentration of oxygen (often 1% or less O2). (wikipedia.org)
  • animal
  • The present study compares the effectiveness of two culture techniques for recovery of Salmonella from environmental samples obtained in a large-animal referral veterinary hospital during a Salmonella outbreak. (scielo.org.za)
  • Cell culture can be an alternative to animal use in some cases. (wikipedia.org)
  • show
  • Regardless of culturing status, the Zetaproteobacteria show up worldwide in estuarine and marine habitats associated with opposing steep redox gradients of reduced (ferrous) iron and oxygen, either as a minor detectable component or as the dominant member of the microbial community. (wikipedia.org)
  • however
  • However, they still have the Interferon-alpha/beta receptor, so they respond normally when interferon from another source is added to the culture. (wikipedia.org)
  • results
  • There were discordant results for 60 samples, including 59 that were only culture-positive using the TBG-RV-XLT4 method, and one sample that was only culture-positive using the SEL-XLD method. (scielo.org.za)
  • Salmonella was much more likely to be recovered using the TBG-RV-XLT4 method, and there appeared to be five times more false-negative results using the SEL-XLD technique. (scielo.org.za)
  • formation
  • Obermüller2017, author={Obermüller, Jakob}, title={Mimicking embryonic interactions with engineered cell-culturing techniques}, abstract={Compaction of the mouse embryo is triggered by the formation of filopodia by some of the blastomeres. (chalmers.se)
  • Several
  • Multiple stamps can be created from a single master Individual stamps can be used several times with minimal degradation of performance A cheaper technique for fabrication that uses less energy than conventional techniques After this technique became popular various limitations and problems arose, all of which affected patterning and reproducibility. (wikipedia.org)