Culture skeletal muscle cells. Medical search. Frequent questions

The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
Accordingly, the volume of SR tubules localized around the myofibrils is strongly reduced in skeletal muscle fibers of 4- and 10-month-old sAnk1 knockout (KO) mice, while additional structural alterations only develop with aging. (mdpi.com)
To verify whether the lack of sAnk1 also alters intracellular Ca 2+ handling, cytosolic Ca 2+ levels were analyzed in stimulated skeletal muscle fibers from 4- and 10-month-old sAnk1 KO mice. (mdpi.com)
Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
In vertebrate skeletal muscle, the vitamin C transporter SVCT2 is preferentially expressed in slow muscle fibers. (nih.gov)
This method provided optically ideal conditions for the study of muscle fibers with oil immersion, phase contrast time-lapse cinematography at 1 frame per minute without apparent damage for periods as long as 10 days. (rupress.org)
Multinuclear muscle fibers have been shown with cine technique to result from both myoblast fusion and polar extension of preformed (explanted) muscle tissue. (rupress.org)
It is suggested that this phenomenon may be responsible for numerous reports of amitosis in the genesis of muscle fibers. (rupress.org)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
Upon injury, FAPs enter the cell cycle and expand to produce cytokines and deposit extracellular matrix (ECM) to enhance differentiation of SCs into muscle fibers. (usz.ch)
Cardinal morphologic features of myofibrillar myopathies on muscle biopsy are vacuolated muscle fibers and inclusions that were initially given different names in the 1970s. (medscape.com)
However, in the mid 1990s, other proteins were also found to accumulate in the abnormal muscle fibers, and molecular genetic studies revealed several chromosomal loci. (medscape.com)

More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
We hypothesize that either small changes in cell composition during ageing may affect the epigenetic age of a tissue, or that our epigenetic clocks for mice need further refinement" says Shahragim Tajbakhsh. (pasteur.fr)
Hit compounds from such screens are not necessarily effective on hypoxic and slowly proliferating cells in 3-D tumour tissue. (researchgate.net)
The similarities between dysfunction seen previously in PBMC and those seen here in muscle cells suggests that CFS is a multi-tissue disease, which reflects what patients report and the symptoms they present with . (phoenixrising.me)
Skeletal muscle satellite cells were obtained by tissue block culture method in combination with pre-plating techniques , and the purity of these cells was detected by both immunocytochemistry and fluorescence activated cell sorter (FACS) with Pax7 as marker of SMSC. (bvsalud.org)
Cells crawled out from the edge of tissue blocks after 1 week of culture . (bvsalud.org)
Skeletal muscle satellite cells with high purity and strong differentiation ability can be obtained by means of tissue block culture method . (bvsalud.org)
However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
Skeletal muscle is the largest tissue in the body. (frontiersin.org)
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
After the cells of interest have been isolated from living tissue , they can subsequently be maintained under carefully controlled conditions. (wikipedia.org)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
This paper provides a step-by-step protocol describing how to handle the tissue, conduct the imaging, and use stereological methods to obtain unbiased and quantitative data on fiber type-specific subcellular glycogen distribution in skeletal muscle. (jove.com)
In addition, as is characteristic to native periosteum, osteoclast precursor cells were imaged and proven to naturally arrange as an outer layer of the mineralized bone tissue construct. (techbriefs.com)
The Biosensors for Bioengineering Group is looking for an Early Stage Researcher to work on the development of 3D skeletal muscle tissue integrated within microfluidic organ-on-chip devices. (ibecbarcelona.eu)
Develop and characterize 3D tissue co-cultures of skeletal muscle tissues from patient-derived cells. (ibecbarcelona.eu)
Muscle regeneration is characterised by a well-timed network of different cell types providing an environment that allows the activation of muscle stem cells, called satellite cells (SCs) to regenerate the damaged tissue. (usz.ch)
Scientists studied skeletal muscle tissue obtained from muscle biopsies of 10 patients with CFS/ME. (wellnessresources.com)
AMPK ( 5'-adenosine monophosphate-activated protein kinase ) is an enzyme switch found in cells throughout the body, especially in the hypothalamus, liver, muscle, adipose tissue and pancreas. (wellnessresources.com)
In skeletal muscle, like brain a post-mitotic tissue, multiple mitochondrial DNA deletions as well as variable deficits in complex I of the mitochondrial respiratory chain (MRC), abnormal calcium handling, and reduced expression of PGC-1α have been described. (bmj.com)
Methods/techniques We used quadriceps muscle tissue from 12-week old R6/2 HD transgenic mice, and near to motor onset pre-manifest HD (n = 20), early onset HD patients (n = 20) and sex and age matched healthy controls (n = 20), as part of the Multi-Tissue Molecular signatures in HD project (MTM-HD). (bmj.com)
Two reports appeared as advance online publications in the top British journal Nature , accompanied by a news report that begins, "The hyped ability of adult stem cells to sprout replacement tissue types is being called into question. (i-sis.org.uk)
Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
The photos below show the damaged jaw muscle tissue after 10 days. (ironmagazine.com)
Skeletal muscle is often the site of tissue injury due to trauma, disease, developmental defects or surgery. (ironmagazine.com)
We conclude that NF-kappaB exerts a role in regulating myogenesis and that modulation of NF-kappaB activity within muscle tissue is beneficial for muscle repair. (ironmagazine.com)

To gain insights into the possible involvement of intracellular vitamin C on early myogenesis, we investigated the regulation of SVCT2 expression in cultures of chick fetal myoblasts. (nih.gov)
SVCT2 expression increases in cultures of both, slow and fast muscle-derived myoblasts, as they fuse to form mainly fast myotubes. (nih.gov)
MLO-Y4 osteocytes were cultured in Alvetex scaffold in either perfused or static conditions (using a perfusion plate or well inserts respectively) and the resulting conditioned medium was added to C2C12 myoblasts. (reprocell.com)
Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
The supplement is combined with a basal medium to prepare MyoCult™-SF Expansion Medium for the culture of primary human and hPSC-derived myoblasts. (stemcell.com)
Myoblasts cultured using MyoCult™-SF Expansion Medium can be differentiated into multinucleated myotubes using MyoCult™ Differentiation Kit (Human) . (stemcell.com)
The EasySep™ Human CD56 Positive Selection Kit II is designed to isolate CD56+ cells from fresh or previously frozen peripheral blood mononuclear cells and human skeletal muscle (myoblasts and fibroblasts) cultures by positive selection. (stemcell.com)

Neither the title of the paper, nor the abstract mentioned that in the experiment, five out of 25 rats receiving the transplant died with "teratoma-like tumors" in their brains, a well-known hazard of ES cells. (i-sis.org.uk)

Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
Seeking understand the decline in function of tissues in organisms, researchers from the Stem Cells and Development unit at Institut Pasteur made a discovery on the still unknown mechanisms of the epigenetic clock in stem cells. (pasteur.fr)
It should be noted that epigenetic clocks based on DNA methylation are constructed from data from multiple tissues from a mixture of different cell types. (pasteur.fr)
They may instead be fusing with existing cells, creating genetically mixed-up tissues with unknown health effects" [1]. (i-sis.org.uk)
The researchers measured the recovery of the damaged tissues by examining the concentration of embryonic myosin heavy chain [EMHC], a marker for muscle recovery. (ironmagazine.com)
PCB methyl sulphones and related compounds: Identification of target cells and tissues in different species. (cdc.gov)

The PromoCell Skeletal Muscle Cell Growth Medium is a low-serum medium (5% V/V) developed for the expansion of human skeletal muscle cell cultures. (promocell.com)
For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)

Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
To establish the isolation, culture and identification methods of primary rat skeletal muscle satellite cells (SMSC) and observe its characterization of differentiation in vitro . (bvsalud.org)
Stem cell behaviour can also be controlled in vitro by manipulating the elasticity of both porous and non-porous materials, yet translation to therapeutic processes in vivo remains elusive. (nature.com)
Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro , and cell deployment in vitro and in vivo , can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. (nature.com)
Figure 3: Controlling cell deployment kinetics from void-forming hydrogels in vitro and in vivo . (nature.com)
Three-dimensional (3D) in vitro culture can improve the performance and physiological relevance of immortalized and primary cells. (reprocell.com)
If this is the kind of results you want to achieve with your in vitro cell models, then adding perfusion can be done with minimal disruption to your existing protocols. (reprocell.com)
In vitro expansion of mesenchymal stem cells using 3-D matrix derived from cardiac fibroblast. (reprocell.com)
Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
To test this hypothesis, pericytes were obtained from healthy or immobilized skeletal muscle and exposed to a hydrogen peroxide load in vitro. (illinois.edu)
The researchers discovered that the protocells were able to produce energy-rich molecules (ATP) via glycolysis and synthesize RNA and proteins by in vitro gene expression, indicating that the inherited bacterial components remained active in the synthetic cells. (nanowerk.com)
Other pharmacological and genetic inhibitors of NF-kappaB also stimulate muscle differentiation in vitro. (ironmagazine.com)

They found that the spontaneous mutation frequency in ES cells is 100-fold lower than that in mouse embryonic fibroblasts (a somatic cell line), which is similar to adult cells in vivo . (i-sis.org.uk)
describe a protocol for the direct reprogramming of mouse fibroblasts into expandable functional skeletal muscle progenitors. (tocris.com)
Mouse embryonic fibroblasts (MEF) or tail tip fibroblasts (TTF) transduced with a doxycycline-inducible lentiviral vector, allowing inducible expression of the transcription factor MyoD, were cultured in MEF medium. (tocris.com)
2018) Direct reprogramming of mouse fibroblasts into functional skeletal muscle progenitors. (tocris.com)
Monolysocardiolipin in cultured fibroblasts is a sensitive and specific marker for Barth Syndrome. (lu.se)

In the present study, we demonstrate that IRE1α (also known as ERN1) and its downstream target, XBP1, are activated in skeletal muscle of mice upon injury. (elifesciences.org)
Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)
Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
By examining the transcriptome and epigenome of individual muscle stem cells simultaneously, we found that DNA transcribes* more chaotically in older muscle stem cells compared to those in younger mice. (pasteur.fr)
This matrix protein is decreased in muscle stem cells in old mice thereby destabilising contact with the fibre. (pasteur.fr)
Mouse ADSC (mADSC) isolated from C57 male mice were expanded for CS formation (10 6 cells per CS). (biomedcentral.com)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
Master thesis projects are available to better understand the role of microRNAs in this process using mouse models for muscle regeneration and work with primary muscle cells from mice and humans. (usz.ch)
Muscle stem cell function is impaired in adult ΔKlotho mice. (figshare.com)
A) The activation potential (number of clusters per myofiber after 72 h of culture divided by the number of muscle stem cells per myofiber directly after isolation) (ΔKlotho n = 5 mice, control n = 7 mice). (figshare.com)
B) Percentage of Pax7+/MyoD- cells within a cluster on myofibers isolated from p42 old mice. (figshare.com)
C) Percentage of Pax7+/MyoD+ cells within a cluster on myofibers isolated from p42 old mice. (figshare.com)
D) Percentage of Pax7−/MyoD− cells within clusters on myofibers isolated from p42 old mice. (figshare.com)
We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
Our laboratory previously demonstrated that muscle-resident perivascular stem cells (NG2+ and CD146+ pericytes), also considered the primary mesenchymal stem cell (MSC) in muscle, can effectively recover muscle mass in young adult mice following a period of immobilization. (illinois.edu)
In Chapter 2, pericytes were isolated based on two cell surface markers (NG2 and CD146) and then transplanted into tibialis anterior muscle (TA) in young adult mice after two weeks of immobilization. (illinois.edu)
Interestingly, extracellular vesicles derived from pericytes in culture were able to significantly improve myofiber size and collagen degradation in both adult and aged mice after immobilization. (illinois.edu)
In that study the researchers inflicted damage on the jaw and calf muscle tibialis of mice by exposing them to extreme cold. (ironmagazine.com)
Mice treated with the optimal dose [20 microgram/kg/day] showed muscle recovery after 4 days that untreated mice only obtained after 20 days. (ironmagazine.com)

In experimental diabetes, significant alterations in contractile properties of skeletal muscle have been described in [ 3 - 5 ], as have increases in intramuscular sorbitol [ 2 ]. (hindawi.com)

In 1907 the zoologist Ross Granville Harrison demonstrated the growth of frog embryonic cells that would give rise to nerve cells in a medium of clotted lymph . (wikipedia.org)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
Britain s House of Lords final approval of therapeutic human cloning and embryonic stem cells research has intensified the battle for ascendancy between adult and embryonic stem cells. (i-sis.org.uk)
This latest anti-publicity on adult stem cells comes on the heels of a paper announcing success in embryonic stem (ES) cell transplant in a Parkinson rat model published in the house journal of the United States National Academy of Sciences [5]. (i-sis.org.uk)
In the current issue of the same journal [6], researchers compared the frequency and type of mutation induced in embryonic stem cells and embryonic somatic cells. (i-sis.org.uk)

On day 0, fibroblast culture medium was exchanged for induced myogenic progenitor cell (iMPCs) medium, which resulted in iMPCs expressing key skeletal muscle stem and progenitor cell markers, by day 19. (tocris.com)

CFS skeletal muscle cells show similar dysfunction in mitochondrial respiration as PBMCs. (phoenixrising.me)
The inability of CFS cells to utilise glucose as a fuel source to the same extent as healthy controls results in decreased mitochondrial respiration at both basal and maximal levels. (phoenixrising.me)
Does impaired mitochondrial function affect insulin signaling and action in cultured human skeletal muscle cells? (ncl.ac.uk)
Insulin-resistant type 2 diabetic patients have been reported to have impaired skeletal muscle mitochondrial respiratory function. (ncl.ac.uk)
To address this, a model of impaired cellular respiratory function was established by incubating human skeletal muscle cell cultures with the mitochondrial inhibitor sodium azide and examining the effects on insulin action. (ncl.ac.uk)
Human mitochondrial antigen (hu-Mito) is a marker for receiver cells. (elifesciences.org)
Aims To assess mitochondrial biogenesis, MRC assembly and MRC function in R6/2 and human skeletal muscle. (bmj.com)
Electrical stimulation prevents doxorubicin-induced atrophy and mitochondrial loss in cultured myotubes [med. (emf-portal.org)

In Chapter 3, single-cell RNA sequencing was conducted on hindlimb muscle two weeks after immobilization to evaluate differential gene expression in pericytes and other mononuclear cells. (illinois.edu)

Myogenesis of these cells was induced in differentiation medium and the mRNA expressions of myogenic differentiation gene (MyoD) and Myogenin were determined by Real-time polymerase chain reaction ( PCR ). (bvsalud.org)
Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies [med. (emf-portal.org)
The striking effects of curcumin on myogenesis suggest therapeutic applications for treating muscle injuries. (ironmagazine.com)

We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration. (nature.com)
These constructs (mm 3 -cm 3 scale) consisted of tens to hundreds of long parallel myofiber bundles, containing densely packed, highly viable, and aligned muscle cells. (nature.com)
We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
While host nerve integration and formation of NMJs were evident within the implanted skeletal muscle constructs, we observed that the constructs did not support the full restoration of defected muscles at 8-week post-implantation, which is likely due to the delayed integration of host nerve. (nature.com)
Administration technique is crucial and among others minimal constructs - cell sheets (CS) have certain advantages. (biomedcentral.com)
Mineralized 3D bone constructs fluorescently stained to track osteoclast precursor cells, revealed as white portions in the image. (techbriefs.com)
After introducing mineralization agents to the culture, the constructs take on a bonelike appearance and have a more rigid structure suitable for being tested. (techbriefs.com)
Enhancing Myoblast Fusion and Myotube Diameter in Human 3D Skeletal Muscle Constructs by Electromagnetic Stimulation [med. (emf-portal.org)
First author Dr Can Xu, Research Associate at the University of Bristol, added: "Our living-material assembly approach provides an opportunity for the bottom-up construction of symbiotic living/synthetic cell constructs. (nanowerk.com)

Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
CS with underlying muscle was stained for apoptosis, proliferation, monocytes and blood vessels. (biomedcentral.com)
Figure 2: Manipulating stem cell osteogenesis and proliferation by controlling the elasticity of the bulk phase of void-forming hydrogels. (nature.com)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
However, it increases proliferation rates in some cell types (e.g. mesenchymal stem cells, osteosarcoma cells, HUVECs) which puts extra demands on nutrient supply and waste removal [1,2,3] . (reprocell.com)
The systems have been shown to improve cell proliferation and specific protein expression beyond that seen for static 3D cultures [4,5,6,7] . (reprocell.com)
The levels of glucose and secreted lactate contained in the cell culture medium were monitored as indicators of metabolic depletion, while protein syntheses and cell proliferation were measured by expression of phosphorylated Ribosomal protein S6 and cyclin D1. (reprocell.com)
Results suggested that perfusion culture reduces hypoglycemia, anaerobic stress, and preserves protein synthesis and cell proliferation. (reprocell.com)
Curcumin can act directly on cultured muscle precursor cells to stimulate both cell proliferation and differentiation under appropriate conditions. (ironmagazine.com)

There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
Skeletal muscle disuse atrophy is the decline of muscle mass that occurs after periods of extended bed rest or immobilization. (illinois.edu)

In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
Remarkably, the nucleated cells do not express osteoblast markers, indicating differentiation to the in vivo cell type known as the osteocyte. (techbriefs.com)
Strikingly, some of these cells display selective template-DNA strand segregation during mitosis in the muscle fibre in vivo, as well as in culture independent of their niche, indicating that genomic DNA strands are nonequivalent. (nih.gov)
Some in vivo transplantation studies have reported robust (35-50%) levels of transdifferentiation, which makes it unlikely that the results are due to cell fusion events. (i-sis.org.uk)
The comprehensive functional tests and histological analysis in vivo indirectly and directly proved the effectiveness of LIPUS for muscle recovery. (ijbs.com)
Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
We show that the kinetics and extent of muscle regeneration in vivo after trauma are greatly enhanced following systemic administration of curcumin, a pharmacological inhibitor of the transcription factor NF-kappaB. (ironmagazine.com)

This study has shown that the ability of CFS skeletal muscle cells to utilise glucose as a substrate is diminished compared to healthy control cells. (phoenixrising.me)
Basal glucose uptake was 325 ± 39 pmol/min/mg (mean ± SE) in untreated cells. (ncl.ac.uk)
For example, endurance exercise training increases the synthesis and activity of hexokinase II [ 6 , 7 ], which increases the capacity of the skeletal muscle cell to phosphorylate glucose with subsequent metabolism through glycolysis. (hindawi.com)
The results showed that CFS muscle cell cultures had no increase in AMPK phosphorylation or glucose uptake after several hours of electrical stimulation. (wellnessresources.com)
When AMPK is turned on, it helps cells burn fat and glucose resulting in ATP production. (wellnessresources.com)
Under aerobic conditions, the activity of PDC determines the rate at which all cells oxidise glucose, pyruvate, and lactate. (bmj.com)

A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. (nature.com)
This discovery on ageing underscores the importance of basic research in understanding the mechanisms that regulate stem cell properties and function to pave the way for future development of innovative diagnostic or therapeutic strategies. (pasteur.fr)
Cells' therapeutic potential can be also increased by expression of proangiogenic factors by viral transduction. (biomedcentral.com)
Our findings show that biophysical cues can be harnessed to direct therapeutic stem cell behaviours in situ . (nature.com)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
They are easy to culture and are commonly used in transfection and large-scale production of therapeutic proteins. (thermofisher.com)
What cell surface marker provided the best means to isolate therapeutic pericytes? (illinois.edu)
The GMAB technology platform, exclusively licensed from Yale University, utilizes a proprietary, cell-penetrating antibody to non-covalently bind to and deliver therapeutic levels of multiple types of nucleic acids, including synthetic RNA (ribonucleic acid) messenger RNA, small interfering RNA, deoxyribonucleic acids, antisense oligonucleotides and gene editing molecules. (biospace.com)
GMAB technology utilizes a novel, cell-penetrating antibody to non-covalently bind to and deliver therapeutic levels of a wide variety of nucleic acid payloads to select cells. (biospace.com)

These findings suggest that SVCT2-mediated uptake of vitamin C could play diverse roles on skeletal muscle development and physiology. (nih.gov)
In 1999 pharmacologists at Emory University published the results of a dramatic experiment in AJP - Cell Physiology, which is perhaps even more convincing than the more sophisticated experiments published later. (ironmagazine.com)

The cells could be made to differentiate into bone forming cells, cartilage forming cells, fat cells, skeletal muscle cells and endothelial cells. (i-sis.org.uk)

Primary Human Skeletal Muscle Cells isolated from different skeletal muscles from adult single donors. (promocell.com)
In the latest skirmish, two rapid online publications are presented as if they were evidence against the existence of adult stem cells. (i-sis.org.uk)
This leaves one with the distinct impression that adult stem cells might not exist, and furthermore, they may be a health hazard. (i-sis.org.uk)
Fusion could also be obtained between ES cells and cells isolated from adult brain. (i-sis.org.uk)
These researchers pointed out that the spontaneous fusion rate (without interleukin-3) was extremely low, between 2-11 per million bone marrow cells, and is unlikely to account for all the findings with adult stem cells. (i-sis.org.uk)
In fact, the senior authors in both reports admit that some adult stem cells may genuinely revert to an earlier stage of development or switch fates [1]. (i-sis.org.uk)
Neither report cited a paper published last year in the journal Blood [4], where a group from the Stem Cell Institute, Department of Medicine, and Cancer Center, University of Minnesota Medical School, Minneapolis, reported the most comprehensive experiments proving that a single adult stem cell can differentiate into all cell types in culture. (i-sis.org.uk)
The finding that adult cells can fuse with ES cells is therefore irrelevant to the debate, and the fact that it was used in an attempt to discredit adult stem cells is itself revealing, particularly in view of other recent findings on ES cells. (i-sis.org.uk)
They are usually adult-onset diseases with slowly progressive weakness involving proximal (and distal) muscles. (medscape.com)
A narrative bibliographic review article was done with the search of original and review articles in international scientific mentales en adultos journals, in English and Spanish listing the relationship between the seroprevalence of T. gondii and the development of mental disorders in the adult population. (bvsalud.org)

These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)

We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)

Spheroids of EMT-6 mammary tumor cells were markedly more resistant to different exposure doses of Adriamycin (ADR) than monolayer cells in exponential or plateau growth phases. (researchgate.net)
ENT2 is highly expressed in multiple tumor types as well as skeletal and cardiac muscle. (biospace.com)

Neurogenesis-on-Chip: Electric field modulated transdifferentiation of human mesenchymal stem cell and mouse muscle precursor cell coculture [med. (emf-portal.org)

Both the protein and mRNA of PTPIP51 have been implicated in various carcinomas, including prostate carcinoma (PCa), keratinocyte carcinoma, basal cell carcinomas, and squamous cell carcinomas. (wikipedia.org)

20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
Supportive care is essential to preserve muscle function, maximize functional ability, and prolong life expectancy. (medscape.com)
Corresponding author Professor Stephen Mann said: "Achieving high organisational and functional complexity in synthetic cells is difficult especially under close-to-equilibrium conditions. (nanowerk.com)
No specific treatment is available for any of the LGMD syndromes, though aggressive supportive care is essential to preserve muscle function, maximize functional ability, and prolong life expectancy. (medscape.com)
Loss of the functional skin such as muscle, nerves, blood vessels and barrier after thermal injury results in in- bone despite the absence of a major cutane- creased susceptibility to infection, which is ous injury. (who.int)

Scadden, D. T. The stem-cell niche as an entity of action. (nature.com)
One of the cell types involved in the regenerative stem cell niche are fibro/adipogenic progenitors (FAPs). (usz.ch)
Using pulse-chase labelling with BrdU to mark the putative stem cell niche, we identify a subpopulation of label-retaining satellite cells during growth and after injury. (nih.gov)

We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)

Western blot analysis of FACS purified Control and αGFP receiver 293 cells cultured in isolation or co-culture with sGFP Tomato-positive sender cells (mouse KPT lung cancer cells) for 24 hr. sGFP but not Tomato is present in touched αGFP receiver cells. (elifesciences.org)

This strategy has been applied to develop a three-dimensional (3D) human muscle model with endothelium specificity and endomysium for the study of fibrosis 22 . (nature.com)
Using BV system and sodium butyrate treatment we expressed human VEGF165 in mADSC (production of VEGF165 reached ≈ 25-27 ng/ml/10 5 cells) and optimized conditions to ensure cells' viability after transduction. (biomedcentral.com)
Incubation of human skeletal muscle cells with 50 and 75 μM azide resulted in 48 ± 3% and 56 ± 1% decreases, respectively, in respiration compared with untreated cells mimicking the level of impairment seen in type 2 diabetes. (ncl.ac.uk)
Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine, rat, canine, and rabbit skeletal muscle cells. (promocell.com)
DetachKit for gentle and effective detachment of adherent primary human cells. (promocell.com)
Human primary osteoblast/osteoclast 3D construct development following 21 days of culture (~4 mm diameter spheroids). (techbriefs.com)
In a separate project, scientists from the University of Oxford cultured the human pancreatic cancer cell line PSN-1 over a period of seven days, in either static or perfused conditions, using the Alvetex perfusion plate . (reprocell.com)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
Immune system Human immunodeficiency virus (HIV) infection results from 1 of 2 similar retroviruses (HIV-1 and HIV-2) that destroy CD4+ lymphocytes and impair cell-mediated immunity, increasing risk of certain. (msdmanuals.com)

Muscle stem cell from young mouse stained with the transcription factor Pax7 and in contact with the muscle fibre through the extracellular matrix protein M-Cadherin. (pasteur.fr)
After purification by pre-plating techniques , more than 97.6% of the cells expressed Pax7, a unique marker of satellite cells . (bvsalud.org)
Finally, we show that template DNA cosegregates with Numb in label-retaining cells that express the self-renewal marker Pax7. (nih.gov)

Interestingly, when sorbitol formation was inhibited by giving an aldose reductase inhibitor (ARI) to streptozotocin- (STZ-) induced diabetic rats, skeletal muscle sorbitol levels were lowered, and muscle contractile properties significantly improved [ 2 ]. (hindawi.com)
We hypothesized that insulin administration would significantly decrease sorbitol accumulation in skeletal muscle. (hindawi.com)
Cell culture techniques were advanced significantly in the 1940s and 1950s to support research in virology . (wikipedia.org)
LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)

Isolate a small specimen from the muscle biopsy or whole muscle which has a maximum diameter of one millimeter in any direction and is longer longitudinally than cross-sectionally. (jove.com)
Muscle biopsy and genetic testing are the most important tools used in the diagnostic evaluation of patients in whom limb-girdle muscular dystrophy (LGMD) is suspected. (medscape.com)
Scalds are the from biopsy cultures [ 6,7 ]. (who.int)

Spatiotemporal magnetic fields enhance cytosolic Ca2+ levels and induce actin polymerization via activation of voltage-gated sodium channels in skeletal muscle cells [med. (emf-portal.org)
Following exposure (24hrs), arteriolar characteristics and reactivity were measured in the gluteus maximus muscle using intravital microscopy (IVM) coupled with microiontophoretic delivery of acetylcholine (ACh) or sodium nitroprusside (SNP). (cdc.gov)

There is a general agreement that hypertrophy is accompanied by the addition of new nuclei from stem cells to help the muscles meet the enhanced synthetic demands of a larger cell. (frontiersin.org)

These problems can be partially circumvented by using macroporous biomaterials that improve the survival of transplanted stem cells and provide molecular cues to direct cell phenotype. (nature.com)

Mesenchymal stem/stromal cells including adipose-derived stromal cells (ADSC) are a promising cell source for this application. (biomedcentral.com)

Systemic administration of the NF-kappaB inhibitor curcumin stimulates muscle regeneration after traumatic injury. (ironmagazine.com)

Dulbecco's Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. (sigmaaldrich.com)
DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. (sigmaaldrich.com)
Figure 1 summarises the major steps of carbohydrate oxidation in mammalian cells. (bmj.com)

As well, many older individuals suffer from sarcopenia, a protracted muscle wasting disorder that typically begins after the age of 50 and involves a loss of about 1% of muscle mass per year ( Woo, 2017 ). (frontiersin.org)

For this reason, the team of Shahragim Tajbakhsh from Institut Pasteur, in collaboration with the team of Wolf Reik at the Babraham (UK) tried to decipher the relationships between epigenetic regulation of DNA and ageing muscle stem cells. (pasteur.fr)

Khetan, S. & Burdick, J. A. Patterning network structure to spatially control cellular remodeling and stem cell fate within 3-dimensional hydrogels. (nature.com)
It will also allow a mechanistic approach in the modulation of cell signaling at the cellular level within the bone matrix. (techbriefs.com)
Limb-girdle muscular dystrophy (LGMD) protein defects occur in several pathways involved in the biologic function of muscle and can be divided into groups based on cellular localization. (medscape.com)

The guideline provides algorithms for diagnosis based on the clinical phenotype, including pattern of muscle involvement, inheritance pattern, age of onset, and associated manifestations (e.g. contractures, cardiomyopathy, respiratory failure). (medscape.com)
Patients with LGMD2B can have a variable MRI picture with involvement of gastrocnemius in Miyoshi myopathy and involvement of glutei as well as anterior and posterior thigh muscle in patients with a LGMD phenotype. (medscape.com)

In later stages of apoptosis the entire cell becomes fragmented, forming a number of plasma membrane-bounded apoptotic bodies which contain nuclear and or cytoplasmic elements. (wikipedia.org)
Effects of three-dimensional culturing on osteosarcoma cells grown in a fibrous matrix: analyses of cell morphology, cell cycle, and apoptosis. (reprocell.com)

Since the initial success of cell therapy for ischemic diseases many attempts have been made to increase its efficacy. (biomedcentral.com)
The measure of glycogen content in single cells and subcellular organizations is important because in most cell types, single cells adapt differently to changing physiological conditions and diseases. (jove.com)
I look forward to working with Gennao Bio on the continued development of the GMAB platform to develop effective therapeutics for the treatment of oncology indications, as well as monogenic skeletal and cardiac muscle diseases. (biospace.com)
Gennao Bio is developing this delivery system with an initial focus on oncology and monogenic skeletal muscle diseases. (biospace.com)

Marrow-derived stem cell motility in 3D synthetic scaffold is governed by geometry along with adhesivity and stiffness. (nature.com)

The Petri dish is one of the most common items in biology laboratories and has entered popular culture . (wikipedia.org)
In this post, we will explore two examples of how perfusion systems can help improve the relevance of your 3D cell culture, based on two publications from Alvetex users in the field of developmental biology and cancer. (reprocell.com)
The cosegregation of 'immortal' template DNA strands and their link with the asymmetry apparatus has important implications for stem cell biology and cancer. (nih.gov)

We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)

Cell culture studies show antibody-mediated delivery of a series of retinoic acid-inducible gene I (RIG-I) ligands, including hairpin RNAs and synthetic double-stranded RNA (poly(I:C)), resulting in robust RIG-I stimulation and induction of type-1 interferon signaling. (biospace.com)

In addition, myoblast cell cultures from pre-manifest and manifest HD subjects showed impaired cell differentiation and HTT inclusions similar to those in brain. (bmj.com)

The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells. (nature.com)

Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
The contract will be within the framework of the MORFEO project (Muscle-on-a-chip approach to reproduce fibrotic processes in engineered 3D co-culture organoids for Muscular Dystrophy): whose main objective is the development of Muscle-on-chip devices to model Col6-related myopathy. (ibecbarcelona.eu)
Limb-girdle muscular dystrophy refers to a group of genetic disorders that cause progressive weakness and wasting of the skeletal muscles, predominantly around the shoulders and hips. (medscape.com)

Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture . (wikipedia.org)

Delivery of CS allows transplantation of cells along with matrix proteins to facilitate engraftment. (biomedcentral.com)
These include proteins associated with the sarcolemma (see image below), proteins associated with the contractile apparatus (see image below), and various enzymes involved in muscle function. (medscape.com)

details] Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: A real-time, single-cell approach [med. (emf-portal.org)

In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)

In stem cells in aged individuals, both transcriptional and epigenetic changes associated with ageing have been observed. (pasteur.fr)
This means that by the age of 80, sarcopenic individuals have lost about 40% of their muscle mass, a key factor in falls, frailty, and nursing home admissions. (frontiersin.org)
therefore sorbitol accumulation is probably not related to muscle dysfunction in insulin-treated diabetic individuals. (hindawi.com)

The purpose of this study was to determine the effects of insulin and of endurance exercise on skeletal muscle sorbitol levels in streptozotocin-induced diabetic rats. (hindawi.com)
Skeletal muscle sorbitol levels were the highest in the diabetic sedentary no-insulin group. (hindawi.com)
The main purpose of this study was to determine the effect of insulin treatment in STZ-induced diabetes on skeletal muscle sorbitol values. (hindawi.com)
0.01), reduced hemoglobin A1c levels (P = 0.01), and improved insulin sensitivity (P = 0.03) and β cell function (P = 0.01). (jci.org)
details] Induction of Hair Growth by Insulin-Like Growth Factor-1 in 1,763 MHz Radiofrequency-Irradiated Hair Follicle Cells [med. (emf-portal.org)

Anatomy54

Organisms19

Diseases13

Chemicals and Drugs95

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes54

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena2

Information Science3

Health Care1

Fibers12

Tissue37

Myoblasts8

Abstract1

Tissues6

Expansion of human skeletal muscle2

Vitro12

Fibroblasts5

Mice21

Contractile properties of skeletal muscle1

Embryonic5

Progenitor1

Mitochondrial8

Mononuclear cells1

Myogenesis3

Constructs10

Proliferation9

Atrophy2

Vivo8

Glucose6

Therapeutic9

Physiology2

Endothelial cells1

Adult10

Acquired by a1

Hypothesis1

Tumor2

Precursor1

MRNA1

Functional5

Stem-cell3

Model of Duchenne muscular1

Isolation1

Human9

Pax73

Significantly4

Biopsy3

Sodium2

Hypertrophy1

Biomaterials1

Mesenchymal1

Inhibitor1

Mammalian3

Sarcopenia1

Regulation1

Cellular3

Phenotype2

Apoptosis2

Diseases4

Scaffold1

Biology3

FACS2

Stimulation1

Myoblast1

Bone marro1

Muscular dystrophy3

Substrate1

Proteins2

Modulation1

Murine3

Individuals3

Insulin5