Culture induced pluripotent stem cells. Medical search. Frequent questions

Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell. (wikipedia.org)
citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
dubious - discuss] In his Nobel seminar, Yamanaka cited the earlier seminal work of Harold Weintraub on the role of myoblast determination protein 1 (MyoD) in reprogramming cell fate to a muscle lineage as an important precursor to the discovery of iPSCs. (wikipedia.org)
iPSCs are typically derived by introducing products of specific sets of pluripotency-associated genes, or "reprogramming factors", into a given cell type. (wikipedia.org)
Unlike the first generation of iPSCs, these second generation iPSCs produced viable chimeric mice and contributed to the mouse germline, thereby achieving the 'gold standard' for pluripotent stem cells. (wikipedia.org)
In previous studies, induced pluripotent stem cells (iPSCs) differentiated into imDCs, and sinomenine (SN) was used to inhibit the maturation of imDCs. (newswise.com)
To study the capacity of SN to maintain iPSC-derived imDCs (SN-iPSCs-imDCs) in an immature state and the mechanism by which SN-iPSCs-imDCs induce immunotolerance. (newswise.com)
In this study, mouse iPSCs were induced to differentiate into imDCs in culture medium without or with SN (iPSCs-imDCs and SN-iPSCs-imDCs). (newswise.com)
The effects of iPSCs-imDCs and SN-iPSCs-imDCs on T-cell stimulatory function, and regulatory T (Treg) cell proliferative function in vitro were analyzed by mixed lymphocyte reaction. (newswise.com)
The induced immunotolerance of SN-iPSCs-DCs was evaluated by treating recipient Balb/c skin graft mice. (newswise.com)
Both iPSCs-imDCs and SN-iPSCs-imDCs were successfully obtained, and their biological characteristics and ability to induce immunotolerance were compared. (newswise.com)
This study focused on exploring sinomenine (SN) to promote differentiation of induced pluripotent stem cells (iPSCs) into imDCs (SN-iPSCs-imDCs), and found SN-iPSCs-imDCs with worse donor-specific T-cell stimulatory function, and higher regulatory T-cell proliferative function in vitro and in vivo to induce high immune tolerance. (newswise.com)
Induced pluripotent stem cells, iPSCs, are mature, differentiated cells, such as skin cells, that are reprogrammed in the laboratory to be similar to undifferentiated embryonic stem cells. (jove.com)
To create iPSCs, mature cells, like skin fibroblasts, are taken from a patient and put into culture. (jove.com)
These cells are also pluripotent like embryonic stem cells-able to produce all cell types-and are therefore called induced pluripotent stem cells (iPSCs). (jove.com)
iPSCs are potentially valuable in medicine, because a patient who needs a particular cell type-for instance, someone with a damaged retina due to macular degeneration-could receive a transplant of the required cells, generated from another cell type in their own body. (jove.com)
To create iPSCs, mature cells such as skin fibroblasts or blood cells from a person are grown in culture. (jove.com)
It is still being studied whether iPSCs are truly equivalent to embryonic stem cells, but they appear to be similar and can produce cells from all three germ layers of the body. (jove.com)
As with other types of stem cells, scientists are learning how to promote the differentiation of specific cell types from iPSCs efficiently, so that the needed cell types can be produced in adequate quantities. (jove.com)
iPSCs have the great advantage that they can be generated from adults suffering from a known disease and then converted ("differentiated") into any cell type in the body. (ca.gov)
Our lab has been tasked with converting control and patient blood or skin cells, collected by scientists throughout the state of California, into high quality iPSCs. (ca.gov)
They called the pluripotent stem cells that they produced induced pluripotent stem cells (iPSCs) because they had induced the adult cells, called differentiated cells, to become pluripotent stem cells through genetic manipulation. (asu.edu)
The differentiation of patient-derived induced pluripotent stem cells (iPSCs) to committed fates such as neurons, muscle and liver is a powerful approach for understanding key parameters of human development and disease. (nih.gov)
Extended culture of DKC1-mutant iPSCs leads to progressive telomere shortening and eventual loss of self-renewal, indicating that a similar process occurs in tissue stem cells in dyskeratosis congenita patients. (nih.gov)
These findings in iPSCs from dyskeratosis congenita patients reveal that undifferentiated iPSCs accurately recapitulate features of a human stem cell disease and may serve as a cell-culture-based system for the development of targeted therapeutics. (nih.gov)
To investigate underlying disease mechanisms, induced pluripotent stem cells (iPSCs) allow the generation of patient-derived neuronal cells in a dish. (biomedcentral.com)
The AD-iPSCs were differentiated into neuronal cells, in order to generate disease-specific protein association networks modeling the molecular pathology on the transcriptome level of AD, to analyse the reflection of the disease phenotype in gene expression in AD-iPS neuronal cells, in particular in the ubiquitin-proteasome system (UPS), and to address expression of typical AD proteins. (biomedcentral.com)
We detected the expression of p-tau and GSK3B, a physiological kinase of tau, in neuronal cells derived from AD-iPSCs. (biomedcentral.com)
Treatment of neuronal cells differentiated from AD-iPSCs with an inhibitor of γ-secretase resulted in the down-regulation of p-tau. (biomedcentral.com)
Highly characterized cryopreserved human colon intestinal organoids derived from integration-free human induced pluripotent stem cells (iPSCs). (sigmaaldrich.com)
The lab employs a diverse range of model systems including patient-derived induced pluripotent stem cells (iPSCs), neuronal cultures and mouse models. (purdue.edu)
Researchers at Michigan Tech and Harvard Medical School have developed a new way to create cardiomyocytes from induced pluripotent stem cells (iPSCs), for improved cardiac cell therapy. (medgadget.com)
iPSCs show significant potential for cardiac regenerative medicine, as researchers can use biochemical cues to "program" them into a cardiomyocyte-like cell, which shows regenerative efficacy in damaged cardiac muscle. (medgadget.com)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)

Since models are limited to study the mechanism of the neurotoxicity of tranylcypromine, here we utilized an in vitro brain organoid culture system which mimics neurogenesis experimentally to investigate the mechanisms by studying the effect of brain development of tranylcypromine and monitoring the brain damage of different concentrations. (frontiersin.org)
In the present study, to recapitulate the pathologies of these disorders in vitro , we established in vitro models by differentiating mature neurons from human induced pluripotent stem cells (hiPSCs) derived from BP and SCZ patient with contributive copy number variations, as follows: two BP patients with PCDH15 deletion and one SCZ patient with RELN deletion. (nih.gov)
Cell culture systems are an important in vitro tool in basic research and essential in drug discovery and development. (jneurology.com)
Since that time, cell culture methods have been greatly advanced, and the two-dimensional (2D) cell culture systems evolved to be the standard in vitro tool. (jneurology.com)
Conclusion: Functional HLCs were generated from human iPS cells, which could be used for autologus hepatocyte transplantation for liver failure and as in vitro model for determining the metabolic and toxicological properties ofdrug compounds. (elsevierpure.com)
Induced pluripotent stem cells are most useful when combined with a high throughput in vitro assay that can recognize hepatotoxicity. (enzolifesciences.com)
The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
This study developed an estimation routine for counting the viable cells in an in vitro fed-batch Chinese hamster ovary cultivation that relies on off-gas information and inlet gas mixture knowledge. (researchgate.net)
The neuronal cells expressed numerous genes associated with sub-regions within the brain thus suggesting the usefulness of our in-vitro model. (biomedcentral.com)
With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)
In vitro methods, based on human primary cells, cell lines, and genetically modified reporter cell lines, have greatly expanded the scope of in vitro toxicology. (brill.com)
Discuss how we can use stem cells combined with biomaterials for in vitro and in vivo applications. (manchester.ac.uk)
We are working to generate red blood cells in vitro from pluripotent stem cells, however the extremely large cell numbers involved pose unique challenges to culture technology and scale-up. (selectbiosciences.com)
The emergence of human induced pluripotent stem cell (hiPSC) technology has allowed researchers to work with SZ patient-derived neuronal and glial cell types in vitro and to investigate the molecular basis of the disorder in a human neuronal context. (lu.se)
During in vitro fertilization, sperm from the man and several eggs from the woman are placed in a culture dish. (msdmanuals.com)
In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)
Cells derived from bone marrow can undergo osteoinduction in vitro in the absence of osteoinductive factors such as bone morphogenetic proteins. (bvsalud.org)

Moreover, tranylcypromine treatment affects neurons and astrocytes, which impairs cell density and arrangement. (frontiersin.org)
Glutamatergic neurons and GABAergic neurons were induced from hiPSCs under optimized conditions. (nih.gov)
Both types of induced neurons from both hiPSCs exhibited similar phenotypes of MAP2 (microtubule-associated protein 2)-positive dendrite shortening and decreasing synapse numbers. (nih.gov)
Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease. (wikipedia.org)
Here, we show that the protein level of ClpP is selectively decreased in αSyn-expressing cell culture and neurons derived from iPS cells of PD patient carrying αSyn A53T mutant, and in dopaminergic (DA) neurons of αSyn A53T mice and PD patient postmortem brains. (springer.com)
Overexpression of ClpP reduces αSyn-induced mitochondrial oxidative stress through enhancing the level of Superoxide Dismutase-2 (SOD2), and suppresses the accumulation of αSyn S129 phosphorylation and promotes neuronal morphology in neurons derived from PD patient iPS cells carrying αSyn A53T mutant. (springer.com)
We focus on innovations for the generation of neurons, astrocytes, oligodendrocytes, microglia as well as endothelial cells and pericytes. (jneurology.com)
HiPSC-derived Neural Stem Cells (L) and Neurons (R) . i-HNSC stained w/ Nestin (neural stem cell marker, green), SOX 2 (stem cell marker, red) & DAPI (nuclear stain, blue). (cellapplications.com)
Unlike many other kinases, GSK3B is believed to be permanently active in resting cells and in neurons without extracellular stimulation and can be inactivated by Ser9 phosphorylation [ 14 ]. (biomedcentral.com)
From the cover: Manganese and rotenone-induced oxidative stress signatures differ in iPSC-derived human dopamine neurons. (purdue.edu)
Ubiquitination and autophagy target proteins via post-translational modifications for regulating the protein homeostasis of amyloid precursor protein (APP) and its harmful byproduct amyloid beta (Aβ) in neurons, but also in other cells of the neurovascular unit including the BBB. (uniklinikum-jena.de)
We study ubiquitination and autophagy in induced pluripotent stem cell-derived brain-capillary endothelial cells, pericytes, astrocytes, microglia, and neurons. (uniklinikum-jena.de)
Here, the stem cells are shown expressing various markers and differentiating into neurons. (livescience.com)
Reprogrammed cells could then develop into a multitude of cell types, including the neurons found in the brain and spinal cord. (livescience.com)
Stem Derived Dopamine Neurons: Will They Replace DBS as the Leading Neurosurgical Treatment for Parkinson's Disease? (lu.se)
Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

The most well-known type of pluripotent stem cell is the embryonic stem cell. (wikipedia.org)
Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). (wikipedia.org)
They hypothesized that genes important to embryonic stem cell (ESC) function might be able to induce an embryonic state in adult cells. (wikipedia.org)
Thus, induced pluripotent stem cells represent a promising combination of adult and embryonic stem cell characteristics. (prnewswire.com)
If one of the infected cells showed G418 resistance, then the scientists would know that one of the twenty-four genes influenced the cell to become an embryonic stem cell-like cell. (asu.edu)
This time, there were twenty-two cell colonies that showed a resistance to G418, meaning that there were colonies in which the cells exhibited embryonic stem cell properties. (asu.edu)
The surgical procedure for delivery of embryonic stem cell-derived endothelial cells to the ischemic hindlimb is demonstrated, with non-invasive tracking by bioluminescence imaging. (jove.com)
We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

Further studies will be needed in order to assess the impact and mechanisms by which AKIT regulates metabolic states in hPCs, and to test if cells grown in this condition are capable to differentiate into relevant therapeutic cell lineages for biomedical research and applications. (bcregmed.ca)
iPS cells can be grown and differentiated into many different brain cell types, as well as 3D brain organoids 1,2 . (sfari.org)
Stem cells have the potential to be grown to become new tissue for use in transplant and regenerative medicine. (globenewswire.com)
These lab-fabricated, fluorescently stained cardiomyocytes-heart muscle cells-exhibit the maturation and functionality of heart cells grown within a heart. (medgadget.com)
Cultured meat, otherwise known as cultivated meat or cell-based meat, is an emerging technology area that uses lab-grown animal cells to create meat products without requiring animal slaughter, potentially avoiding the environmental problems of conventional agriculture. (idtechex.com)
Over the last five years, cultured meat has grown from almost nothing to over 50 companies racing to bring the first products to market, with over $600 million having been invested in the space. (idtechex.com)
Now scientists have taken fibroblasts from the scalps and the brain linings of 146 human brain donors and grown induced pluripotent stem cells from them as well. (livescience.com)
whole well image of culture well (96 well plate format) containing human induced pluripotent stem cells grown on mouse feeders, stained for pluripotent markers NANOG (red) and OCT4 (green). (lu.se)
If successful, this proposed work could have an enormous impact on regenerative medicine, leading the way to rigorously tested universal-donor stem cells that could be grown and differentiated into very large numbers of cells, made widely available to all medical institutions and used on demand to treat patients with type 1 diabetes and a variety of degenerative diseases, he concluded. (medscape.com)

These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. (wikipedia.org)
Objective: To generate a homogeneous population of patient-specific hepatocyte-like cells (HLCs) from humaniPS cells those show the morphologic and phenotypic properties of primary human hepatocytes. (elsevierpure.com)
The goal of CIRM Grant ID1-06557 is to generate high quality induced pluripotent stem cells (iPSC) from blood and skin samples from 3000 donors, many of whom suffer from untreatable medical conditions, and place them in a Repository accessible to scientists around the world. (ca.gov)
Unguided differentiation confirms HiPSC ability to generate cell derivatives of tissues arising from the three embryonic layers. (cellapplications.com)
The demand for stem cell manufacturing has also been rising as stem cells are being used to generate healthy cells and replace diseased cells or in the development of regenerative medicine. (globenewswire.com)
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
This presentation will describe the current status of strategies to generate induced pluripotent stem (iPS) cells from mouse and human somatic cells for patient-specific disease modeling and cell therapeutic applications. (selectbiosciences.com)

The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
It is also clear that pro-mitotic factors such as C-MYC/L-MYC or repression of cell cycle checkpoints, such as p53, are conduits to creating a compliant cellular state for iPSC reprogramming. (wikipedia.org)
First year progress on grant ID1-06557, " Generation and Characterization of High-Quality, Footprint-Free Human Induced Pluripotent Stem Cell (iPSC) Lines From 3000 Donors to Investigate Multigenic Disease" has met all agreed-upon milestones. (ca.gov)
For example, iPSC lines from patients with heart disease can be converted into heart cells, iPSC lines from patients with Alzheimer's disease can be converted to brain cell, and iPSC lines from patients with pulmonary fibrosis can be converted into cells of the lung. (ca.gov)
Though the current process for toxicity testing largely evolves around the use of in vivo animal work, more and more studies rely on human induced pluripotent stem cell (iPSC)-derived hepatocytes as a viable and up-and-coming alternative of determining toxicity. (enzolifesciences.com)
Induced pluripotent stem cells represent one of the most promising research advances within the past decade, making this a valuable report for both executives and investors to use to optimally position themselves to sell iPSC products. (prnewswire.com)
3dGRO ™ Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. (sigmaaldrich.com)

9 Institute for Stem Cell Biology and Regenerative Medicine and Department of Pathology, Stanford University School of Medicine, Stanford, California 94305. (nih.gov)
Pluripotent stem cells hold promise in the field of regenerative medicine. (wikipedia.org)
Researchers continue to advance knowledge on stem cells and their applications in transplant and regenerative medicine. (globenewswire.com)
Stem cell-based regenerative medicine shows much promise. (selectbiosciences.com)
Induced pluripotent stem (iPS) cells have great potential for regenerative medicine and gene therapy. (stemcell.com)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
You can put islet cells into someone with type 1 diabetes and they can leave the hospital 3 days later with no diabetes….But you're not going to get very far with human donors," press briefing moderator Gordon C Weir, MD, professor of medicine at Harvard Medical School, and cohead of the Joslin Diabetes Center's section on islet cell and regenerative biology, told Medscape Medical News . (medscape.com)

At the workshop, leaders in the field, including several SFARI Investigators, discussed best practices for the generation and characterization of the SFARI iPS cell lines, and they brainstormed ways in which iPS cell models can be used to aid our understanding of autism spectrum disorder (ASD) and other neurodevelopmental conditions. (sfari.org)
Cell Characterization. (cellapplications.com)
Viral and non-viral generation and characterization of induced pluripotent stem cells from human amniotic fluid cells. (mpg.de)

In the experiments, Gurdon conducted nuclear transplantation, or cloning, of differentiated cells, or cells that have already specialized to become one cell type or another, in tadpoles. (asu.edu)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Stem cell transplantation is the removal of stem cells (undifferentiated cells) from a healthy person and their injection into someone who has a serious blood disorder. (msdmanuals.com)
Overview of Transplantation Transplantation is the removal of living, functioning cells, tissues, or organs from the body and then their transfer back into the same body or into a different body. (msdmanuals.com)
But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
These stem cells are most often used for transplantation. (msdmanuals.com)
The idea is that these will be analogous to the O-negative "universal-donor" blood type and could be employed for all cell-based transplantation therapies in patients with immune rejection, said Dr Cowan. (medscape.com)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases (such as Krabbe disease), including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses as well as peroxisome disorders such as X-linked adrenoleukodystrophy. (medscape.com)
Although longitudinal natural history data are limited, published guidelines are available to assist with decisions related to the pursuit of transplantation and whether to use bone marrow or umbilical cord blood-derived cells. (medscape.com)
Some evidence indicates that at least in certain disorders, combination ERT and hematopoietic stem cell transplantation together might be superior to hematopoietic stem cell transplantation alone in patients who are appropriate candidates. (medscape.com)
The availability of both ERT and hematopoietic stem cell transplantation has prompted ongoing consideration of newborn screening efforts to diagnose lysosomal storage diseases. (medscape.com)
Accumulated data indicate that hematopoietic stem cell transplantation may be effective under optimal conditions in preventing the progression of central nervous system symptoms in neuronopathic forms of lysosomal storage diseases, including some of the mucopolysaccharidoses, oligosaccharidoses, sphingolipidoses, and lipidoses. (medscape.com)

However, scientists have found a way to reprogram these mature cells so that they "de-differentiate" and return to an unspecialized, proliferative state. (jove.com)
In addition, their "unspecialized" nature allows them to differentiate into a wide variety of specialized cell types. (prnewswire.com)
While the appeal of embryonic cells has been their ability to differentiate into any type of cell, there has been significant ethical, moral, and spiritual controversy surrounding their use. (prnewswire.com)
HiPSC differentiate into cell derivatives from the 3 embryonic layers: Neuronal marker beta III tubulin (TUJ1), Smooth Muscle Actin (SMA) and Hepatocyte Nuclear Factor 3 Beta (HNF3b). (cellapplications.com)
These iPS cells differentiate into beating cardiomyocytes. (ca.gov)
Human intestinal organoids are self-organizing, 3D structures that can be expanded in long-term culture and differentiate into intestinal epithelial cell types. (sigmaaldrich.com)
Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)

Here, we identified tranylcypromine, which is used to treat refractory depression, caused human-induced pluripotent stem cell-derived brain organoids neurotoxicity, leading to decreased proliferation activity and apoptosis induction. (frontiersin.org)
Immature dendritic cells (imDCs) play an important role in the induction of donor-specific transplant immunotolerance. (newswise.com)
Immature dendritic cells (imDCs) play an important role in the induction of donor-specific transplant immune tolerance. (newswise.com)
A transcriptional roadmap to the induction of pluripotency in somatic cells. (mpg.de)
Several possible mechanisms were considered for the induction of human leukemia, such as clastogenic damage to circulatory stem cells. (who.int)
Objective: This study evaluated the osteogenic induction of human bone marrow cells by human recombinant bone morphogenetic protein-4 (rhBMP-4) and proteins released by Saos-2 (human osteosarcoma cell line). (bvsalud.org)

Our findings provide novel insights into the mechanism underlying αSyn-induced neuronal pathology, and they suggest that ClpP might be a useful therapeutic target for PD and other synucleinopathies. (springer.com)
Transcriptome analysis of AD-iPS derived neuronal cells revealed significant changes in the expression of genes associated with AD and with the constitutive as well as the inducible subunits of the proteasome complex. (biomedcentral.com)
Moreover, an AD-related protein interaction network composed of APP and GSK3B among others could be generated using neuronal cells differentiated from two AD-iPS cell lines. (biomedcentral.com)
Alzheimer's disease (AD) is characterized by histopathological changes, designated as senile plaques and fibrillary deposits, which ultimately lead to the death of neuronal cells in particular in the cerebral cortex of the brain [ 1 , 2 ]. (biomedcentral.com)
Further, we highlight different examples of how these models have shown alterations in neurogenesis, neuronal maturation, neuronal connectivity and synaptic impairment as well as mitochondrial dysfunction and dysregulation of miRNAs in SZ patient-derived cultures compared to controls. (lu.se)
Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)

Preparation of mouse embryonic fibroblast cells suitable for culturing human embryonic and induced pluripotent stem cells. (mpg.de)

Rising stem cell research and increasing demand for stem cell therapies are driving the growth of this segment. (globenewswire.com)
3 Today, serologic and molecular techniques, along with laboratory information systems and electronic health records, contribute to precise blood product management and personalized transfusion therapies, particularly benefiting complex patients with sickle cell disease, thalassemia, and other diseases requiring chronic transfusion support. (cap.org)
As such, this work could help lead to novel stem cell therapies and shed light on a variety of mental disorders, such as schizophrenia , autism and bipolar disorder, which may stem from problems with development, researchers say. (livescience.com)
Cells from corpses might play a key role in developing future stem cell therapies . (livescience.com)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

In 2006, Kazutoshi Takahashi and Shinya Yamanaka reprogrammed mice fibroblast cells, which can produce only other fibroblast cells, to become pluripotent stem cells, which have the capacity to produce many different types of cells. (asu.edu)
Next, Takahashi tried to insert into a fibroblast cell multiple retroviral factors instead of one at a time. (asu.edu)
The researchers added all of the twenty-four retroviral factors at the same time into mouse fibroblast cells. (asu.edu)
Researchers produced pluripotent stem cells from the fibroblast cells in the brain lining of human corpses. (livescience.com)

Our objective is to produce a cell-based test for LQTS with induced pluripotent stem (iPS) cell technology, which allows adult cells to be "reprogrammed" to be stem cell-like cells. (ca.gov)
Gurdon's experiment showed that differentiated adult cells could be induced to an undifferentiated state, where they could once again become multiple cell types. (asu.edu)

IPS cells derived from individuals with neurodevelopmental conditions provide a unique tool to examine early developmental changes in molecular and cellular mechanisms within the human genetic context, thus complementing studies that use human postmortem brain tissue and animal models. (sfari.org)
For example, retinal cells that could be used to replace damaged retinal tissue. (jove.com)
All equipment, including tissue culture safety cabinets and incubators, liquid-handling robotics, and QC instrumentation have been installed and qualified. (ca.gov)
Induced pluripotent stem cell-derived hepatocytes have a primary tissue-like phenotype, constant and indefinite disposal, and the potential to establish genotype-specific cells from different individuals, which make them an interesting means of predicting drug-induced toxicity. (enzolifesciences.com)
An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
The possibilities arising from these characteristics have resulted in great commercial interest, with potential applications ranging from the use of stem cells in reversal and treatment of disease, to targeted cell therapy, tissue regeneration, pharmacological testing on cell-specific tissues, and more. (prnewswire.com)
Short telomeres, a hallmark of dyskeratosis congenita, impair tissue stem cell function in mouse models, indicating that a tissue stem cell defect may underlie the pathophysiology of dyskeratosis congenita. (nih.gov)
By examining the process of stem cells turning into cells in bones, heart muscle, nerves, and other organs and tissue, researchers can better understand how diseases and conditions develop. (globenewswire.com)
New areas of study include the effectiveness of using human stem cells that have been programmed into tissue-specific cells to test new drugs. (globenewswire.com)
Cell and tissue banking applications will account for the leading share of the market by 2020. (globenewswire.com)
Unlike many of today's meat alternatives, cultured meat has the potential to create a product that is completely identical to conventional meat, containing exactly the same cells and tissue. (idtechex.com)
In other words, dead people can yield living cells that can be converted into any cell or tissue in the body. (livescience.com)
Mature cells can be made or induced to become immature cells, known as pluripotent stem cells , which have the ability to become any tissue in the body and potentially can replace cells destroyed by disease or injury. (livescience.com)
Fibroblasts are the most common cells of connective tissue in animals, and they synthesize the extracellular matrix, the complex scaffolding between cells. (livescience.com)
Dispase is suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. (thomassci.com)
Neutral protease (Dispase®) is designed for primary cell isolation and tissue dissociation. (thomassci.com)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

The fibroblasts were engineered so that any cells reactivating the ESC-specific gene, Fbx15, could be isolated using antibiotic selection. (wikipedia.org)
however, expression profiles from hPCs cultured in AKIT and common culture systems had significant differences in gene expression profiles, which were attributed to variable regulation of the expressed genes. (bcregmed.ca)
Each gene was inserted near the mouse Fbx15 gene, a gene that embryonic stem cells express during development in mice. (asu.edu)
His lab utilizes a combined approach of molecular genetics, pharmacology, biochemistry, cell and developmental biology to understand the role of gene-environment interactions between metal exposure and neurodevelopmental and neurodegenerative diseases including Parkinson's Disease, Alzheimer's Disease and Huntington's Disease. (purdue.edu)
The LARGE principle of cellular reprogramming: lost, acquired and retained gene expression in foreskin and amniotic fluid-derived human iPS cells. (mpg.de)
The micropatterning alters the cell cytoskeleton and nuclear shape of cells cultured in the molds, causing changes in gene expression. (medgadget.com)
Using degenerated primers designed from Hamamotoa singularis (Hs) bglA gene, we newly identified three genes that encode cell-bound ß-glycosidase from Sirobasidium magnum (Sm), Rhodotorula minuta (Rm), and Sterigmatomyces elviae (Se). (bvsalud.org)
These experiments are closely linked to epigenetic analysis and gene editing experiments performed in cell culture models of glioma. (lu.se)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
Immunogenicity is reduced by removing the major histocompatibility complex molecules from the stem cells with gene editing, and tolerance induced via "immune editing. (medscape.com)

These cells are also stable in culture, and display reliable and reproducible performance. (enzolifesciences.com)

Recent breakthroughs in human pluripotent stem cells (hPSCs)-derived cerebral organoids offer a promising approach for investigating the mystery of human brain ( 19 ). (frontiersin.org)

Her research focuses on microglia, the immune cells of the brain, where she has developed protocols to immunophenotype microglia in humans using markers relating to a specific function allowing us to investigate the role of these cells in different neurological conditions. (southampton.ac.uk)
The researchers plan to test the cells in an animal model of cardiac disease, and also investigate the potential of enhancing electrical conductivity between the cells, to facilitate the cell-cell communication that occurs in the heart. (medgadget.com)
We also use human induced pluripotent stem cells to investigate how polymorphic transposable elements contribute a genetic component to these disorders, using X-linked dystonia Parkinsonism as our current focus. (lu.se)

Spheroids and complex organoid models allow the formation of self-assembled cell aggregates recapitulating the cellular organization and functionality of specific tissues or organs 8-10 . (jneurology.com)
One critical aspect of optical imaging is the 3D localization of molecular composites in tissues or cells, typically by using specific antibodies with fluorescent reporters. (nature.com)
Systems toxicology, therefore, aims at understanding and exploring the way that different biological components are orchestrated as an ensemble in cells, tissues, and organisms. (brill.com)
Studying how induced pluripotent stem cells develop into various tissues could also shed light on disorders that are due to malfunctions in development. (livescience.com)
Researchers hope to use stem cells to repair or replace cells or tissues damaged or destroyed by such disorders as Parkinson disease, diabetes, and spinal injuries. (msdmanuals.com)
Histiocytoses encompass a group of diverse proliferative disorders characterized by the accumulation and infiltration of variable numbers of monocytes, macrophages, and dendritic cells in the affected tissues. (medscape.com)

Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. (wikipedia.org)
Reprogramming triggers a cascade of evident changes in the host cells that are recognizable morphologically and through a combination of markers and pluripotency assays. (cellapplications.com)
The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. (sigmaaldrich.com)

Recent breakthroughs in human pluripotent stem cell-derived cerebral organoids provide a valuable platform for investigating the human brain after different drugs treatments and for understanding the complex genetic background to human pathology. (frontiersin.org)
In this project we use induced pluripotent stem cell lines from various primate species to perform direct comparative analysis in stem cell derived cultures such as cerebral organoids. (lu.se)

Novel culture and cryopreservation systems for human pluripotent stem cells and neural stem cells will be presented and their advantages and applicability in the production of high quality advanced therapeutic products or functional screening tools for preclinical research discussed. (selectbiosciences.com)

Traditionally, scientists have worked with both embryonic and adult stem cells for research tools, as well as for cellular therapy. (prnewswire.com)
Yamanaka received the Nobel Prize in Physiology or Medicine in 2012, along with John Gurdon, as their work showed scientists how to reprogram mature cells to become pluripotent. (asu.edu)
Takahashi and Yamanaka's 2006 and 2007 experiments showed that scientists can prompt adult body cells to dedifferentiate, or lose specialized characteristics, and behave similarly to embryonic stem cells (ESCs). (asu.edu)
The Dolly experiment showed that scientists could reprogram the nucleus of somatic cells by transferring the contents of the nucleus into oocytes that have had their nuclei removed, a technique called somatic cell nuclear transfer (SCNT). (asu.edu)
Now scientists have harvested such cells from the scalps and brain linings of human corpses and reprogrammed them into stem cells. (livescience.com)
For decades scientists have been trying to reproduce cell culture environment for the closest mimicry of the natural cellular "embedding" within a living organism. (selectbiosciences.com)
Scientists are developing ways of enabling (inducing) other cells (such as a blood or skin cell) to act as stem cells. (msdmanuals.com)

Etoc noted the emphasis that RUMI Scientific has placed on micropatterning technologies 3,4 , which can standardize stem cell differentiation and the geometry of organoids by providing a lattice often comprised of extracellular matrix components. (sfari.org)
These 3D cell culture systems range from scaffold and scaffold-free techniques to more complex systems like organoids. (jneurology.com)
Epithelial intestinal organoids, often referred to as enteroids or "mini-guts", maintain the physiological characteristics of the gastrointestinal system and have been a useful cell culture tool to model intestinal development and disease including the study of colon cancer, celiac disease, inflammatory bowel diseases and host microbiome interactions. (sigmaaldrich.com)
Organoids can be propagated from patient biopsies and from embryonic (ES) and induced pluripotent stem cells (iPS cells). (sigmaaldrich.com)
Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1. (sigmaaldrich.com)

Cadaver-collected fibroblasts can be reprogrammed into induced pluripotent stem cells using chemicals known as growth factors that are linked with stem cell activity. (livescience.com)
2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
One way we did this was by converting adult fibroblasts, or skin cells, into interneurons. (lu.se)

These 2D cell culture systems have already provided many insights into basic cellular functions, biological mechanisms and various disease processes. (jneurology.com)
Human induced pluripotent stem cells--from mechanisms to clinical applications. (mpg.de)
However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)

To facilitate this type of research in the autism field, SFARI has supported the generation of numerous iPS cell lines from individuals with genetic variants in high-confidence autism risk genes, who enrolled in the Simons Simplex Collection (SSC) and Simons Searchlight studies. (sfari.org)
Then, typically, genes for transcription factors, are delivered by viral vectors into the cell nuclei, where they are incorporated into the genome. (jove.com)
The transcription factors then turn on genes that are expressed by embryonic stem cells. (jove.com)
Then, genes for multiple transcription factors are delivered into the cells using a viral vector, and the transcription factor proteins are expressed using the cell's machinery. (jove.com)
The transcription factors then turn on many other genes that are expressed by embryonic stem cells, returning the cells to an undifferentiated, proliferative, and pluripotent state. (jove.com)
After these experiments with somatic cells, Takahashi and Yamanaka hypothesized that there were common factors, genes in particular, which caused somatic cells to become pluripotent stem cells. (asu.edu)
In 2006, Takahashi and Yamanaka selected twenty-four candidate genes as factors that they hypothesized could possibly induce somatic cells to become pluripotent, and they began to test them one at a time. (asu.edu)
The standard tests for LQTS use animal models or hamster cells that express human heart genes at high levels. (ca.gov)
By triggering certain genes, researchers may be able to cause the stem cells to specialize and become the cells that need to be replaced. (msdmanuals.com)
One way to induce these cells is to inject them with material that affects their genes, a process called reprogramming. (msdmanuals.com)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

In most cases, the mechanism of drug-induced LQTS is unknown. (ca.gov)

For many years the musculature of vertebrates was known to derive from the somites, but the exact developmental lineage of axial and limb muscle progenitor cells remained a mystery until Nicole Le Douarin and Charles P. (asu.edu)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)

Efficient generation of nonhuman primate induced pluripotent stem cells. (stemcell.com)

While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. (mdpi.com)

Yamanaka claimed that Gurdon's work in reprogramming mature cells in frogs ( Xenopus ) in 1962 influenced his own work in reprogramming differentiated cells. (asu.edu)
Successfully reprogramming induced pluripotent stem cells so they behave like the cells they are meant to replace means that samples of the mimicked cells must be present for comparison. (livescience.com)
Reprogramming of differentiated cells can be efficiently induced in oocytes. (selectbiosciences.com)

Blood is preferred to bone marrow as a source because the procedure is less invasive and the number (count) of blood cells returns to normal more quickly. (msdmanuals.com)
The bone marrow and blood of children and adults contain stem cells. (msdmanuals.com)
Normal histiocytes originate from pluripotent stem cells, which can be found in bone marrow. (medscape.com)
Study design: Osteoinduction in the presence or absence of Saos-2 and/or rhBMP-4 was evaluated in cultured human bone marrow cells. (bvsalud.org)
Results: Bone marrow cells were adherent since the first day of culture and were positive for osteonectin. (bvsalud.org)
Osteoblasts were obtained from human bone marrow cells even in the absence of growth factors and showed a typical morphology. (bvsalud.org)
Conclusions: This study showed that an osteoblastic cell lineage may be obtained from human bone marrow derived from adherent cells, and that the presence of the rhBMP-4 seems to have an effect during the first stages of differentiation only. (bvsalud.org)

However, these cells have limitations, such as rapid maturation and a short lifespan in vivo . (newswise.com)
and (IV) Maturation by culture in dexamethasone. (elsevierpure.com)

Deficiency in ClpP induces an overload of mitochondrial misfolded/unfolded proteins, suppresses mitochondrial respiratory activity, increases mitochondrial oxidative damage and causes cell death. (springer.com)
Lysosomes are mainly involved in the degradation of extracellular material that enter the cell by endocytosis but are also involved in the homeostasis of other organelles and cellular proteins, including mitophagic and autophagic processes in an age dependent manner. (uniklinikum-jena.de)
They are easy to culture and are commonly used in transfection and large-scale production of therapeutic proteins. (thermofisher.com)

Drug-induced LQTS is the single most common reason for drugs to be withdrawn from clinical trials, causing major setbacks to drug discovery efforts and exposing people to dangerous drugs. (ca.gov)
Despite the critical need, current tests for drug-induced LQTS are far from perfect. (ca.gov)
These studies will provide the foundation for an expanded panel of iPS cell lines from people with other genetic mutations and from people who have no genetically defined risk factor but still have potentially fatal drug-induced LQTS. (ca.gov)

We discuss the pros and cons of these models and describe the potential of using such models for deciphering the contribution of specific human neural cell types to the development of the disease. (lu.se)

If ultimately successful, both approaches would offer access to an unlimited number of implantable islets, representing a significant advance over the multiple human deceased donors that are currently required for islet-cell transplants to be undertaken in humans. (medscape.com)

The Simons Foundation Autism Research Initiative (SFARI) hosted a virtual workshop on March 14 and 18, 2022, to discuss the best practices for the development and use of induced pluripotent stem (iPS) cell models for autism research. (sfari.org)
As of July 2022, the collaboration between SFARI and NLMFF has led to the generation of iPS cell lines from an additional ~60 participants, with plans to establish iPS cell lines from approximately 200 individuals with a variety of autism-linked neurodevelopmental conditions by the end of 2022. (sfari.org)

Especially in drug discovery and development, 2D cell culture systems are essential concerning compound testing and high-throughput screening (HTS) assays. (jneurology.com)
In drug discovery and development, 3D cell culture systems are more frequently used in HTS assays 13,14 . (jneurology.com)
We are now evaluating these LQTS cell lines in cellular assays. (ca.gov)
35 mm culture dishes, pre-tested for optimal colony growth without supporting anchorage-dependent cells in methylcellulose-based assays. (stemcell.com)

Such a description excludes diseases in which infiltration of these cells occurs in response to a primary pathology. (medscape.com)
Improved understanding of the pathology of histiocytic disorders requires knowledge of the origins, biology, and physiology of the cells involved. (medscape.com)
However, patient-derived cells may be more prone to develop disease-associated pathology after grafting. (lu.se)
Lastly, we evaluated the strategy of knocking out a-syn as a means to protect the cells from transfer of pathology upon grafting. (lu.se)

Before using investigational drugs in people, researchers use some types of stem cells to test the drugs for safety and quality. (globenewswire.com)
The unit will demonstrate various types of stem cells: adult, embryonic, induced pluripotent and their specialised culture methods. (manchester.ac.uk)
Introduce students to various types of stem cells, their differentiation capabilities and their potential applications. (manchester.ac.uk)

Progressing a cell-based therapeutic from pre-clinical stage production through later clinical stages and into commercial production requires scale-up, which is one of the chief drivers for the stem cell manufacturing market growth. (globenewswire.com)
The opportunities and challenges of development of stem cell-based therapeutic interventions for joint surface repair for the treatment and prevention of osteoarthritis will be discussed. (selectbiosciences.com)

Our long-term goal is to develop a panel of iPS cell lines that better represent the genetic diversity of the human population. (ca.gov)
These iPS cell lines will be used to refine testing conditions. (ca.gov)
This growing panel of iPS cell lines should allow for testing drugs for LQTS more effectively and accurately than any current test. (ca.gov)
Acute manganese treatment restores defective autophagic cargo loading in Huntington's disease cell lines. (purdue.edu)
Komor, Badran and Liu, 2017 ) have opened up tremendous opportunities for the development of cell lines, especially those of human origin ( Tobita, Guzman-Lepe and de L'Hortet, 2015 ). (brill.com)
Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
The Omicron S-bearing virus robustly escapes vaccine-induced humoral immunity, mainly due to mutations in the receptor-binding motif (RBM), yet unlike naturally occurring Omicron, efficiently replicates in cell lines and primary-like distal lung cells. (biorxiv.org)
The team's current research focus is to create "universal-donor" pluripotent stem-cell lines that are induced to grow into insulin-producing beta cells. (medscape.com)

In 1962 researcher John Bertrand Gurdon at the University of Oxford in Oxford, England, conducted a series of experiments on the developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. (asu.edu)
Gurdon's first experiment in 1958 showed that the nuclei of Xenopus cells maintained their ability to direct normal development when transplanted. (asu.edu)
The cell nuclei is stained with DAPI (blue). (lu.se)

For physiologically relevant disease modeling, three-dimensional (3D) cell culture systems are of great importance because they provide a more representative in vivo -like micro-environment to the cells. (jneurology.com)
However, 2D cell cultures do not represent the physiological in vivo microenvironment of the cells 2 . (jneurology.com)
Usually, a homogenous cell population is cultured as a monolayer, while in vivo the cells are interacting with a heterogenous cell population. (jneurology.com)
These different 3D cell culture systems provide a more representative in vivo -like microenvironment which influences cellular features like morphology, proliferation, differentiation and migration 11,12 . (jneurology.com)

This technology enables the differentiation of the brain cell types of interest, also from diverse patient-derived material. (jneurology.com)

The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)

체세포 핵 치환 (Somatic-cell nuclear transfer, SCNT)은 난자 의 핵 을 제거한 후에, 체세포 의 핵을 이식하여 복제 를 하는 기술을 말한다. (wikipedia.org)

Post-thawing viability of HiPSCs is typically higher than 70%, and HiPSC have demonstrated coherent pluripotent behavior over more than 60 passages. (cellapplications.com)

Conditions such as Huntington's disease, Parkinson's disease, and spinal cord injuries are examples of clinical applications in which stem cells could offer benefits in halting or even reversing damage. (prnewswire.com)

Shinya Yamanaka was awarded the 2012 Nobel Prize along with Sir John Gurdon "for the discovery that mature cells can be reprogrammed to become pluripotent. (wikipedia.org)
Induced pluripotent stem cells were first generated by Shinya Yamanaka and Kazutoshi Takahashi at Kyoto University, Japan, in 2006. (wikipedia.org)

2 iPS Cell-Based Drug Discovery, Drug Research Division, Sumitomo Dainippon Pharma. (nih.gov)
BCREGMED is proud to bring experts and trainees together to support and advance global stem cell research. (bcregmed.ca)
The development of this chemically defined and growth-factor-medium will contribute to promoting large-scale hPCs production and their use in research, cell therapy, and drug discovery while maintaining lower costs and lower variability than those observed with current culture systems. (bcregmed.ca)
This innovation founded a new research field allowing the observation and study of growing and differentiating cells outside of an organism. (jneurology.com)
This milestone opens the door to a potential increase in cell-based models within pharmacological research in the coming years. (jneurology.com)
In particular, Cellular Dynamics International (CDI) has taken lease to approximately 5000 square feet of lab space at the Buck Institute for Research on Aging in Novato, CA. The majority of this space is located within the new CIRM-funded Stem Cell Research Building at the Buck Institute and was extensively reconfigured to meet the specific needs of this grant. (ca.gov)
Stem cell research and experimentation have been in process for well over five decades, as stem cells have the unique ability to divide and replicate repeatedly. (prnewswire.com)
A distinctive feature of this report is an end-user survey of 273 researchers (131 U.S. / 143 International) that identify as having induced pluripotent stem cells as a research focus. (prnewswire.com)
Yamanaka worked to find new ways to acquire embryonic stem cells to avoid the social and ethical controversies surrounding the use of human embryos in stem cell research during the late twentieth and early twenty-first centuries. (asu.edu)
Other research groups such as Masako Tada's group in Japan in 2001 and Chad CowanÆs group in Massachusetts in 2005 combined embryonic stem cells with somatic cells to produce pluripotent cells. (asu.edu)
Major factors driving market revenue growth include increasing use of stem cells to treat various diseases, rising stem cell-based research activities, and growing funding from private and public organizations for stem cell-based research. (globenewswire.com)
Stem cell manufacturing involves production of stem cells that can be used in cell-based research and therapeutics development. (globenewswire.com)
This report provides an in-depth technology and industry evaluation of the cultured meat market based on extensive primary research into the sector, including interviews with numerous key players. (idtechex.com)
Andreas' research focuses on generating brain cells, specifically interneurons, in the lab, which has significant implications for the study of neurodegenerative and neuropsychiatric disorders. (lu.se)
Combining cell biology and electrophysiology, his work has the potential to create personalized disease models for future research. (lu.se)

In addition, in physiological conditions the cells are interacting with multiple extracellular matrix (ECM) components, which can actively affect the behavior of the cells 3 . (jneurology.com)
The mechanical properties of substrates play an important role in the cell behavior because the mechanical cues that cells sense in the actual (heart) environment is unique," said Abadi. (medgadget.com)

The team developed a culture mold that mimics the physiological conditions under which cardiomyocytes grow. (medgadget.com)
Induced pluripotent stem cell-derived cardiomyocytes beating in substrate. (medgadget.com)
As the cells mature, they begin to beat, and more closely resemble mature, native cardiomyocytes. (medgadget.com)

Anatomy52

Organisms12

Diseases10

Chemicals and Drugs54

Analytical, Diagnostic and Therapeutic Techniques and Equipment57

Phenomena and Processes51

Disciplines and Occupations9

Technology, Industry, Agriculture3

Humanities1

Information Science2

Health Care1

IPSCs35

Vitro18

Neurons20

Embryonic stem8

Grown9

Generate7

IPSC7

Regenerative7

Characterization3

Transplantation12

Differentiate7

Induction6

Neuronal6

Mouse embryonic1

Therapies5

Fibroblast4

Adult cells2

Tissue18

Gene11

Reproducible1

HPSCs1

Investigate3

Tissues6

Markers3

Cerebral organoids2

Neural Stem1

Scientists7

Organoids5

Fibroblasts3

Mechanisms3

Genes11

Mechanism1

Progenitor4

Primate1

Molecular Biology1

Reprogramming3

Bone marrow7

Maturation2

Proteins3

Drug-induced3

Human neural1

Donors1

20222

Assays4

Pathology4

Types of stem cells3

Therapeutic2

Lines14

Nuclei3

Vivo4

Patient-derived1

Lineage2

Somatic cell nuc1

Viability1

Clinical applications1

Shinya Yamanaka2

Research15

Behavior2

Cardiomyocytes3