• Chlamydomonas
  • Fresh Chlamydomonas culture (blue) grown in TAP growth medium and Chlamydomonas cultures previously frozen (green and red lines) using the GeneArt® Cryopreservation Kit for Algae and resuscitated in TAP growth medium following the manufacturer's recommendations were monitored at OD 750 over an 80-hour period (50 µE, 24°C, 110 rpm shaker). (thermofisher.com)
  • Growth was consistent in all three conditions, demonstrating the utility of the GeneArt® Cryopreservation Kit for Algae to produce frozen Chlamydomonas samples that, when resuscitated, have growth characteristics similar to those of fresh aliquots. (thermofisher.com)
  • PROTOCOLS
  • Development of effective cryopreservation protocols will be essential to realizing the potential for clinical application of neural stem and progenitor cells. (ingentaconnect.com)
  • Current cryopreservation protocols have been largely employed in research, which does not require as stringent consideration of viability and sterility. (ingentaconnect.com)
  • Therefore, these protocols involve the use of serum and protein additives, which can potentially introduce contaminants, and slow cooling with DMSO/glycerol-based cryopreservation solutions, which impairs cell survival. (ingentaconnect.com)
  • This sterile method for the xeno-free cryopreservation of murine neurospheres without animal or human proteins may have the potential to serve as a starting point for the development of cryopreservation protocols for human neural stem and progenitor cells for clinical use. (ingentaconnect.com)
  • Scientist
  • Prior to the companys' founding, its prospective staff members have already had some experience in cryopreservation, when in 2003, Igor Artyukhov acted as the chief advisor to a project of preservation of the brain of one deceased biotech scientist. (wikipedia.org)
  • frozen
  • The prior art generally taught that, once cryopreservation was performed once on hepatocytes, the cells would not remain viable for further testing if frozen a second time. (lexology.com)
  • Traditional cryopreservation has relied on coating the material to be frozen with a class of molecules termed cryoprotectants. (wikipedia.org)
  • DMSO
  • In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use. (atcc.org)
  • viability
  • Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. (atcc.org)
  • Finally, the Court explained that this case differs because the art taught that cryopreservation steps could only be performed once the cellular viability was compromised, and the claimed process was directed to repetition of cryopreservation steps. (lexology.com)
  • Viability assays are used to assess the success of cryopreservation techniques, the toxicity of substances, or the effectiveness of substances in mitigating effects of toxic substances. (wikipedia.org)
  • cell
  • By default it should be considered that cryopreservation alters or compromises the structure and function of cells unless it is proven otherwise for a particular cell population. (wikipedia.org)
  • optimal
  • Cryopreservation is the optimal choice for the preservation and long-term storage of algae since it minimizes genetic drift, facilitates strain and clone exchange between labs, and helps reduces labor and costs. (thermofisher.com)
  • cells
  • Researchers show that tissues are more likely than single cells to suffer damage during cryopreservation because of the tight junctions between cells. (the-scientist.com)
  • On July 5, 2016, the Federal Circuit vacated and remanded a district court decision holding that U.S. Patent No. 7,604,929 , which is directed to a method of cryopreservation of hepatocytes (liver cells) was invalid under 35 U.S.C. § 101 because it was directed to a patent-ineligible concept. (lexology.com)
  • Effective Cryopreservation of Neural Stem or Progenitor Cells Wit. (ingentaconnect.com)
  • cardiac
  • A cryonicist wishing immediate cryopreservation thus must induce a state of cardiac and respiratory arrest or clinical death-suicide in the eyes of the law-before the procedure can begin. (alcor.org)
  • research
  • These experiments all provide evidence supporting the conclusion "that brain structure and even many brain functions are likely to be reasonably well preserved by freezing in the presence of cryoprotective agents," and ushering in a new era of research in cryopreservation of the brain. (alcor.org)
  • Alcor accepts bodies as "anatomical donations" under the Uniform Anatomical Gift Act and Arizona Anatomical Gift Act for research purposes, reinforced by a court finding (Alcor, Merkle & Henson v. Mitchell) in its favor that affirmed a constitutional right to donate one's body for research into cryopreservation. (wikipedia.org)
  • long term
  • Cryopreservation (storage in liquid nitrogen (LN) at −196°C) is the only currently available long-term storage technique that ensures safe conservation of genetic resources of valuable plant species [ 14 - 16 ]. (hindawi.com)
  • services
  • Cryovita was a for-profit organization which provided cryopreservation and transport services for Alcor in the 1980s until Leaf's death, at which time Alcor began providing these services on its own. (wikipedia.org)
  • legal
  • To best counter such a physical threat, one wishes to have cryopreservation performed electively, that is, undergo proactive legal death, when by reasonable biomedical criteria the time is right. (alcor.org)
  • As of 2014, about 250 bodies were cryopreserved in the United States, and 1,500 people had made arrangements for cryopreservation after their legal death. (wikipedia.org)