• Chlamydomonas
  • Fresh Chlamydomonas culture (blue) grown in TAP growth medium and Chlamydomonas cultures previously frozen (green and red lines) using the GeneArt® Cryopreservation Kit for Algae and resuscitated in TAP growth medium following the manufacturer's recommendations were monitored at OD 750 over an 80-hour period (50 µE, 24°C, 110 rpm shaker). (thermofisher.com)
  • Growth was consistent in all three conditions, demonstrating the utility of the GeneArt® Cryopreservation Kit for Algae to produce frozen Chlamydomonas samples that, when resuscitated, have growth characteristics similar to those of fresh aliquots. (thermofisher.com)
  • Scientist
  • Prior to the companys' founding, its prospective staff members have already had some experience in cryopreservation, when in 2003, Igor Artyukhov acted as the chief advisor to a project of preservation of the brain of one deceased biotech scientist. (wikipedia.org)
  • cell
  • Cryopreservation of cultured cells is an important step in the workflow of researchers that often requires trial and error in order to achieve satisfactory levels of cell viability and recovery after thawing. (atcc.org)
  • In addition to cell culture grade DMSO, ATCC also offers a line of serum-free cryopreservation media that are hassle-free and ready-to-use. (atcc.org)
  • Additional cell lines/clones can be expanded and grown in sufficient quantities to produce 4 vials for cryopreservation. (rockland-inc.com)
  • By default it should be considered that cryopreservation alters or compromises the structure and function of cells unless it is proven otherwise for a particular cell population. (wikipedia.org)
  • process
  • The Warwick team, led by Professor Matthew I. Gibson, has developed a new method for cryopreservation, inspired by the process by which organisms known as extremophiles, survive in some of the coldest regions on Earth. (warwick.ac.uk)
  • optimal
  • Cryopreservation is the optimal choice for the preservation and long-term storage of algae since it minimizes genetic drift, facilitates strain and clone exchange between labs, and helps reduces labor and costs. (thermofisher.com)
  • cardiac
  • A cryonicist wishing immediate cryopreservation thus must induce a state of cardiac and respiratory arrest or clinical death-suicide in the eyes of the law-before the procedure can begin. (alcor.org)
  • Techniques
  • Viability assays are used to assess the success of cryopreservation techniques, the toxicity of substances, or the effectiveness of substances in mitigating effects of toxic substances. (wikipedia.org)
  • research
  • These experiments all provide evidence supporting the conclusion "that brain structure and even many brain functions are likely to be reasonably well preserved by freezing in the presence of cryoprotective agents," and ushering in a new era of research in cryopreservation of the brain. (alcor.org)
  • Alcor accepts bodies as "anatomical donations" under the Uniform Anatomical Gift Act and Arizona Anatomical Gift Act for research purposes, reinforced by a court finding (Alcor, Merkle & Henson v. Mitchell) in its favor that affirmed a constitutional right to donate one's body for research into cryopreservation. (wikipedia.org)
  • services
  • Cryovita was a for-profit organization which provided cryopreservation and transport services for Alcor in the 1980s until Leaf's death, at which time Alcor began providing these services on its own. (wikipedia.org)
  • legal
  • To best counter such a physical threat, one wishes to have cryopreservation performed electively, that is, undergo proactive legal death, when by reasonable biomedical criteria the time is right. (alcor.org)
  • As of 2014, about 250 bodies were cryopreserved in the United States, and 1,500 people had made arrangements for cryopreservation after their legal death. (wikipedia.org)