2. Crosslinking Proteins to PEG Hydrogels - Injectable laminin-functionalized hydrogel for nucleus pulposus regeneration, Biomaterials, 2013 (34): 7381-7388, Â Text . (creativepegworks.com)
3. Crosslinking Proteins to PEG Hydrogels - Photocrosslinkable laminin-functionalized polyethylene glycol hydrogel for intervertebral disc regeneration, Acta Biomaterialia 10 (2014) 1102-1111, Â Text . (creativepegworks.com)
Nucleic acids2
UV crosslinking MAGNA Nylon is an excellent way to attach nucleic acids. (thomassci.com)
PAXgene Tissue reagents in prefilled containers and PAXgene Tissue Kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acids for molecular research analysis. (qiagen.com)
Functional groups2
In this work, we tested the procedures to crosslink functional groups in polar domains of humic acids by using carbodiimide coupling and analyzed to which extent influenced the modification their hydration properties and stability. (researchgate.net)
Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. (creativepegworks.com)
Polymers3
Storage: -20C Application: TRUEPEG is used to crosslink PVA and dextran polymers to generate stable hydrogel. (thomassci.com)
Epichlorohydrin-crosslinked CD-polymers (CDPs) have also been effectively prepared in the ball mill. (beilstein-journals.org)
Furthermore, a class of non-hydrolysable, soluble and insoluble CD polymers (CDP) can be prepared by crosslinking CDs with epichlorohydrin or 1,2:3,4-diepoxybutane. (beilstein-journals.org)
Hydrogel2
Application: EncapGel(TM)-Cx Kit is a ready-to-use, chemically crosslinked hydrogel kit for the encapsulation and delivery of drugs, biomolecules, and cells. (thomassci.com)
Acrylate-PEG-SCM may be used to introduce an amine reactive functional group in crosslinked PEG hydrogel. (creativepegworks.com)
Moisture1
Higher moisture uptake and water holding capacity were observed in humic acids, which were pre-wetted prior to crosslinking for at least 24 h. (researchgate.net)
Lead2
Characterization of prepared derivatives showed that both methods lead to formation of crosslinked humic structures. (researchgate.net)
Such crosslinks lead to nucleic acid degradation during tissue fixation, storage, and processing. (qiagen.com)
Storage1
The system consists of a fixation reagent (PAXgene Tissue FIX), a stabilization reagent (PAXgene Tissue STABILIZER), prefilled containers for tissue collection, storage, and transportation, and kits for purification of RNA, DNA, or total RNA, including miRNA. (qiagen.com)
Properties2
Using of EDC resulted in lower degree of crosslinking, but better hydration properties. (researchgate.net)
The high- f junctions can provide load-bearing abilities similar to a covalent cross-link while retaining the ability to self-repair and concurrently confer stimuli-responsive properties arising from the high- f junction species. (aip.org)
High2
This work presents a proof of concept for a green cyclodextrin derivatisation method that uses low-boiling epoxide reagents in a high-energy ball mill (HEBM). (beilstein-journals.org)
We provide a canonical introduction to dual-junction-functionality associative polymer networks, which combine high and low functionality ( f ) dynamic cross-link junctions to impart load-bearing, dissipation, and self-repairing ability to the network. (aip.org)
Work1
This is then a good example of the classical theory of cross-fertilization at work. (lu.se)
Polymer5
Standard, cross-linked and highly cross-linked agarose beads (without ligand linking) are used in Gel Filtration Chromatography (or size Exclusion Chromatography), while, for affinity chromatography, ligands such as nitrilotriacetic acid (NTA) or iminodiacetic acid (IDA) are covalently linked to the agarose bead polymer which can be further used for separation of tagged proteins from other proteins. (zellx-biochem.de)
In an effort to broaden the utility of the industrially used ROMP polymer polydicyclopentadiene, we introduced an ester-functionalized dicyclopentadiene monomer that can be polymerized and crosslinked under similar conditions to those of the unfunctionalized parent, and for which the resulting functionalized polymer ( f PDCPD) maintains the thermal and mechanical properties that make polydicyclopentadiene useful. (rsc.org)
To address the low surface energy of PDCPD, and to bestow additional function to the polymer, we installed a C -linked ester group on the dicyclopentadiene monomer. (rsc.org)
18-20 The ester-functionalized monomer ( f DCPD) engages in Ru-catalyzed ROMP similarly to the parent dicyclopentadiene, to afford a linear telechelic polymer that can then be thermally cured to provide a densely crosslinked, functionalized form of polydicyclopentadiene that we call f PDCPD ( Fig. 1 ). (rsc.org)
Further improvements in this area such as, introduction of high throughput synthesizers, better coupling reagents, improved polymer supports, newer sets of protecting groups for exocyclic amino groups of nucleic bases and introduction of universal polymer supports have completely revolutionized the entire field of nucleic acids chemistry. (researchgate.net)
Hyaluronic acid3
Therefore, we synthesize carrier-free drug NPs containing cis-aconitic anhydride-modified doxorubicin and paclitaxel (CAD-PTX) and coating with crosslinked (CL) surfactant based on hyaluronic acid (HA) segment. (medscape.com)
and (d) optionally working up the dispersion of crosslinked hyaluronic acid microbeads obtained in step (c). (justia.com)
The present invention relates to a process for the preparation of modified hyaluronic acid (HA), in particular crosslinked HA in emulsion, for use in cosmetic and personal care applications. (justia.com)
Mass spectrometry1
Mass spectrometry in three dimensions (MS3D) is a newly developed method for the determination of protein structures involving intramolecular chemical crosslinking of proteins, proteolytic digestion of the resulting adducts, identification of crosslinks by mass spectrometry (MS), peak assignment using theoretical mass lists, and computational reduction of crosslinks to a structure by distance geometry methods. (nih.gov)
Protein3
The addition of trifunctional coupling agents causes incomplete crosslinking and introduces branching architecture to the protein molecules. (frontiersin.org)
We have investigated the interactions of two nuclear-encoded preproteins with the chloroplast protein import machinery at three stages in import using a label-transfer crosslinking approach. (rupress.org)
The inner membrane proteins, Tic(21) and Tic22, and a previously unidentified protein of 14 kD are the major targets of crosslinking during the late stages in import. (rupress.org)
Gels1
No. 4,582,865 (Biomatrix Inc.) describes the preparation of crosslinked gels of HA, alone or mixed with other hydrophilic polymers, using divinyl sulfone (DVS) as the crosslinking agent. (justia.com)
Provide2
To facilitate the unambiguous identification of crosslinked peptides from proteolytic digestion mixtures of crosslinked proteins by MS, we introduced double 18O isotopic labels into the crosslinking reagent to provide the crosslinked peptides with a characteristic isotope pattern. (nih.gov)
To facilitate the unambiguous identification of crosslinked peptides from proteolytic digestion mixtures of crosslinked proteins by MS, we introduced double 18 O isotopic labels into the crosslinking reagent to provide the crosslinked peptides with a characteristic isotope pattern. (uky.edu)
Agent1
We have designed and tested both in vitro and in vivo , a monoamine oxidase B (MAOB) specific prodrug, PAM-OBG, that is converted by glioma MAOB into the MGMT inhibitor O 6 -benzylguanine (O 6 BG) and the DNA crosslinking agent acrolein. (oncotarget.com)
Conditions1
suggesting that a portion of the outer and inner membrane import machineries may be stably linked under steady state conditions. (rupress.org)
Structure1
Fig. 1 Structure of linear and thermally crosslinked ester functionalized PDCPD. (rsc.org)
Network1
1-5 The extensive crosslinking network within PDCPD contributes to high heat and impact resistance, 6-8 making it an attractive material for further applications including composite materials, 9-11 aerogels, 12,13 and self-healing polymers. (rsc.org)
Solution1
It is majorly used as a prepolymer solution that can be used in the formation of a cross-linked polymeric system. (sigmaaldrich.com)
Process1
Previous research has attempted to control the biodegradation process by varying the cross-linking density or by providing competitive substrates for proteases. (uhasselt.be)