Correlate gene expression thymidylate synthase. Medical search. Frequent questions

We tested if routine immunohistochemical markers can be used to distinguish putative Pemetrexed responders, predicted by gene signatures, from nonresponders. (eur.nl)
Two of the subclasses correlated to putative Pemetrexed resistance. (eur.nl)
In addition, the identified signature genes characterizing putative Pemetrexed responsiveness predicted therapeutic benefit in a subset of squamous cell carcinoma. (eur.nl)
T . equi has lost the putative genes for host cell transformation, or the genes were acquired by T . parva and T . annulata after divergence from T . equi . (biomedcentral.com)

Click on genes, proteins and metabolites below to link to respective articles. (wikipedia.org)
Deletion or mutation of genes coding for Ku70 or Ku80 proteins results in a highly radiosensitive phenotype [10]. (termedia.pl)
More common are small, subtle changes of the DNA sequence that can alter the structure and function of the proteins encoded by genes. (healthdrugpdf.com)
The genes that encode proteins comprise only a tiny fraction of human DNA, even though they represent the major biological function of the genome. (healthdrugpdf.com)
An educated guess is made regarding the characteristics of a gene involved in HIV resistance (for example only cell-surface proteins will be examined as they may bind HIV). (healthdrugpdf.com)

We also analyzed the predictive or prognostic value of germline polymorphisms of candidate genes associated with 5-FU and oxaliplatin. (biomedcentral.com)
Ten polymorphisms within five genes were investigated including TS, GSTP, ERCC, XPD and XRCC. (biomedcentral.com)
Several polymorphisms in TS may influence TS mRNA transcription, stability, or protein expression. (biomedcentral.com)
Patients were genotyped for a panel of 192 tagging polymorphisms in 34 immune-related genes. (frontiersin.org)
Many genetic typing methods use DNA sequencing approaches, but others use specific gene probes, single-strand conformation polymorphism (SSCP), or restriction fragment length polymorphisms (RFLP). (cdc.gov)
As mentioned above, most polymorphisms are irrelevant for the gene function. (healthdrugpdf.com)
Pharmacogenomics has been perceived as a useful tool to predict treatment response as well as clinical prognosis in cancer patients, and numerous studies have suggested the functions of single nucleotide polymorphisms (SNPs) in affecting drug sensitivity by modifying relevant genes [ 4 ]. (hindawi.com)
Consequently, it is necessary to identify functional gene polymorphisms as new biomarkers for accurately predicting clinical outcomes of chemotherapy in NSCLC, which could also reduce the side effects and provide the most cost-effective approach for individuals [ 3 , 4 ]. (hindawi.com)

It is a tumor suppressor gene, involved in DNA DSB repair through the process of homologous recombination [16]. (termedia.pl)

0.0001), although no correlation was observed between E2F1 gene expression and the apoptotic index. (aacrjournals.org)

Several biomarkers have been used to predict treatment responses, such as the EGFR mutation status for targeted therapy or the excision repair cross-complementation group 1 (ERCC1) and thymidylate synthase (TS) expression for conventional chemotherapy [ 3 - 8 ]. (medsci.org)
Moreover, HOTAIR expression correlates with differential response of ovarian cancer cells to platinum-based chemotherapy [ 7 ]. (oncotarget.com)
The present study established a docetaxel-resistant gastric cancer cell line and evaluated the alteration of mRNA expression levels globally by microarray, in an attempt to obtain novel predictive biomarkers for docetaxel chemotherapy. (iiarjournals.org)
Two most common SNPs of ERCC1 gene are C118T (rs11615) with a C → T substitution at exon 4 and C8092A (rs3212986) with a C→A change in the 3′-untranslated region, both of which have been speculated to regulate the ERCC1 mRNA expression and manage the NER pathway, consequently affecting the efficacy of platinum-based chemotherapy [ 7 ]. (hindawi.com)
To date, lots of studies regarding gene variants that may alter response and tolerability to chemotherapy drugs have been carried out, including both retrospective and perspective studies. (hindawi.com)
Thymidylate synthase expression and response to neoadjuvant chemotherapy in patients with advanced head and neck cancer. (uchicago.edu)

The present study evaluated the prognostic value of the epidermal growth factor receptor (EGFR) mutation status, and excision repair cross-complementation group 1 (ERCC1) and thymidylate synthase (TS) expression following intercalated tyrosine kinase inhibitor (TKI) therapy and platinum- and pemetrexed-based chemotherapies (subsequent second-line treatment) for patients with adenocarcinoma non-small-cell lung cancer (AC-NSCLC). (medsci.org)
The EGFR mutation status and ERCC1 and TS expression were evaluated through direct DNA sequencing and immunohistochemical analyses, respectively. (medsci.org)
The EGFR mutation status and ERCC1 and TS expression were the significant predictors of clinical outcomes. (medsci.org)
In conclusion, the present results indicate that the EGFR mutation status and TS and ERCC1 expression can be used as the predictors of OS after subsequent second-line treatments for AC-NSCLC. (medsci.org)
We analyzed the mutation frequencies of common actionable genes and their association with clinicopathological characteristics and oncologic outcomes using targeted NGS. (preprints.org)

Methods: A global expression profile-based molecular classification of non-small cell lung cancer (NSCLC) was performed. (eur.nl)
A variety of typing methods have been used for Pneumocystis genetic analysis, and a large number of gene loci have been examined. (cdc.gov)
We focus on the methods and genes that have been most widely used for molecular epidemiologic analyses or have the greatest potential application. (cdc.gov)
Materials and Methods: Microarray analysis was performed to explore various gene expression levels between parental and docetaxel-resistant cells. (iiarjournals.org)

Sequence analysis of the thymidylate synthase (TS) and superoxide dismutase (SODA) gene loci, the EPSP synthase domain of the multifunctional arom gene, and the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) locus have been used to distinguish Pneumocystis species from diverse mammalian hosts ( 6 , 20 , 21 ). (cdc.gov)
In turn, each one of these genes may have deviations from the published sequence. (healthdrugpdf.com)
After the candidate genes are identified one must determine which ones have unusual sequence in a resistant individual. (healthdrugpdf.com)
Although genetics have played a dominant role in cancer research, epigenetics (heritable changes in gene function that do not involve alterations in DNA sequence) has become equally important in this field. (aacrjournals.org)

Human cytomegalovirus infection induces cellular thymidylate synthase gene expression in quiescent fibroblasts. (unito.it)
Central to the epigenetic control of gene expression is the histone code through which input signals can be translated into a heritable pattern of gene expression defining cellular output states ( Fig. 1 ). (aacrjournals.org)

Single-strand selective monofunctional uracil DNA glycosylase is an enzyme that in humans is encoded by the SMUG1 gene. (wikipedia.org)
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. (cancerindex.org)
5-FdUMP inhibits thymidylate synthase (key enzyme in DNA synthesis), which leads to accumulation of dUMP, which then gets misincorporated into the DNA in the form of 5-FdUTP resulting in inhibition of DNA synthesis and function with cytotoxic DNA strandbreaks. (medscape.com)

Such a general screening will yield literally thousands of candidate genes that must be examined. (healthdrugpdf.com)

MicroRNAs (miRNAs), single-stranded RNA molecules with 19~25 nucleotides in length, are recognized as key modulators governing multiple gene expression via binding to the 3' untranslated region (3'-UTR) of target mRNA at the posttranscriptional level [ 7 ]. (oncotarget.com)

In this phenotype, widespread methylation of CpG islands results in the epigenetic inactivation of tumor suppressor genes by promoter methylation. (biomedcentral.com)
Undergoes hydrolysis in liver and tissues to form the active moiety (fluorouracil), inhibiting thymidylate synthetase, which in turn blocks methylation of deoxyuridylic acid to thymidylic acid. (medscape.com)
In mammalian cells, histone H3 Lys 9 (H3K9) methylation and histone hypoacetylation are usually associated with methylated DNA, heterochromatin, and gene silencing. (aacrjournals.org)
Histone hyperacetylation and H3K4 methylation are associated with unmethylated DNA, euchromatin, and gene expression ( Fig. 2 ). (aacrjournals.org)

These medicines have been looked at in terms of their detailed chemical structures, their proteomic properties, the molecular biological pathways through which they work, their gene activation and epigenetic properties, their pharmacological properties, etc, This work has generally been of high quality and has resulted in thousands or tens of thousands of research reports, many of them published in highly respected Western journals. (anti-agingfirewalls.com)
Recent advances in understanding the role that epigenetics plays in cancer pathogenesis and understanding the mechanisms through which these processes regulate gene expression have stimulated considerable interest in developing clinically viable antineoplastic agents that target enzymatic components of transcriptional regulatory complexes responsible for the establishment of pathologic epigenetic modifications that lead to deregulated gene expression in cancer. (aacrjournals.org)

The aim of this study was to explore predictive genes involved in docetaxel sensitivity of gastric cancer. (iiarjournals.org)
Histoculture drug response assay (HDRA) was used to examine docetaxel sensitivity, while qRT-PCR was used to measure the mRNA levels of the genes in 25 surgically dissected gastric cancer specimens. (iiarjournals.org)
Results: Only CXCR4 mRNA levels in gastric cancer tissues were correlated with docetaxel sensitivity (R 2 =0.23, p=0.019) and significantly higher in resistant specimens (p=0.038). (iiarjournals.org)
Conclusion: CXCR4 mRNA expression levels may be a potential predictive biomarker in gastric cancer. (iiarjournals.org)

Ren L, Liu J, Gou K, Xing C. Copy number variation and high expression of DNA topoisomerase II alpha predict worse prognosis of cancer: a meta-analysis. (jcancer.org)
TOP2A (DNA topoisomerase II alpha) gene, mapped to chromosome 17q12-q21, covers approximately 27.5 kb and includes 35 exons, encoding a 170 kDa protein [ 7 ]. (jcancer.org)

TS and ERCC1 expression levels were effective prognostic factors for negative and positive EGFR-mutated AC-NSCLC, respectively. (medsci.org)
Although the prognostic role of AR in TNBC remains controversial, many studies revealed that a lack of AR expression confers a more aggressive disease course. (biomedcentral.com)
Expression profiles analysis identifies the values of carcinogenesis and the prognostic prediction of three genes in adrenocortical carcinoma. (cancerindex.org)

The relatively frequent resistance of squamous cell carcinoma to Pemetrexed is correlated with high levels of thymidylate synthase expression. (eur.nl)
Conclusions: Gene expression signatures can be used to identify NSCLC subgroups and have potential to predict resistance to Pemetrexed therapy. (eur.nl)
At the current state of technology it is still an immense task to identify genes that might confer a given trait such as resistance to HIV. (healthdrugpdf.com)
It has been documented that high expression of ERCC1 could block the platinum efficacy and cause drug resistance [ 6 ]. (hindawi.com)
Our results demonstrated that miR-31reduced significantly in GBC cells rendering resistance to cisplatin, and upregulated expression of miR-31 augmented chemosensitivity, presenting a therapeutic potential to overcome drug resistance in GBC. (oncotarget.com)

Immunohistochemistry was done to investigate the protein expression of E2F1, TS, and Survivin. (aacrjournals.org)
Scope includes mutations and abnormal protein expression. (cancerindex.org)

Gefitinib and erlotinib would be effective for tumors with epidermal growth factor receptor (EGFR) mutations or increased EGFR gene copy numbers ( 6 ). (aacrjournals.org)
There are only a few reports on the expression of Ku70/80 in breast cancers [17-21], but the association of this protein with germline CHEK2 mutations in breast cancer patients is unknown. (termedia.pl)
Mutations in the gene in coding BRCA1- associated protein 1 (BAP1) have been shown to accelerate asbestos induced mesothelioma and is associated with a syndrome consisting of familial cancers in humans including malignant Mesothelioma and uveal melanomas. (standardofcare.com)

Low SMUG1 expression is also associated with BRCA1, ATM, XRCC1, implying genomic instability in SMUG1 low tumors. (wikipedia.org)
The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)
Therefore comparative genomic analysis of T . equi was undertaken to: 1) identify genes contributing to immune evasion and persistence in equid hosts, 2) identify genes involved in PBMC infection biology and 3) define the phylogenetic position of T . equi relative to sequenced apicomplexan parasites. (biomedcentral.com)
Comparative genomic analysis of T . equi revealed the phylogenetic positioning relative to seven apicomplexan parasites using deduced amino acid sequences from 150 genes placed it as a sister taxon to Theileria spp . (biomedcentral.com)

Cell apoptosis and DDP-chemosensitivity was remarkably increased by knockdown of Src proto-oncogene (Src) expression, which was subsequently reversed by rescue of Src expression in miR-31-expressing cells. (oncotarget.com)
The effect of regulated expression of the miRNA on cell migration, invasion, proliferation and apoptosis was examined by wound healing, transwell assays, CCK-8 assays, colony formation and flow cytometry assays, respectively. (oncotarget.com)
Receptor interacting protein 1 kinase (RIPK1) is a key mediator of not only a process of regulated necrosis, termed necroptosis, but also promotion of caspase-8-dependent apoptosis and pro-inflammatory gene expression 13 . (nature.com)

The present study revealed that E2F1 gene expression correlates with TS and Survivin gene expressions and tumor proliferation. (aacrjournals.org)

Following this, the differentially expressed genes (DEGs) were included in Gene Ontology enrichment, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, protein‑protein interaction network and survival analyses. (cancerindex.org)

HSPD1 expression was evaluated by immunohistochemistry from formalin-fixed paraffin-embedded tissues from NSCLC patients. (biomedcentral.com)

Large differences are due to loss or gain of entire genes, gene parts as well as sequences that regulate the gene expression. (healthdrugpdf.com)
The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway evaluation suggested that furthermore to Wnt and changing growth aspect (TGF) signaling pathways, that have been reported in response to follicle formation broadly, another mixed band of signaling pathways that regulate lipid synthesis and fat burning capacity, like the phosphatidylinositol signaling program and glycerolipid signaling and fat burning capacity, are Gemzar biological activity in charge of follicle formation also. (clinical-research-informatics.com)

PLP functions as a coenzyme of 5-aminolevulinic acid synthase, which is involved in the synthesis of heme , an iron -containing component of hemoglobin . (oregonstate.edu)

In addition low SMUG1 transcripts were shown to be potentially correlated with poor survival and linked to aggressive phenotype in breast cancer. (wikipedia.org)
Investigation of SMUG1 expression in gastric cancers showed that overexpressed SMUG1 was correlated with patients' poor survival. (wikipedia.org)
Alteration (amplification or deletion) of TOP2A gene was also significantly related to shorter survival of cancer patients. (jcancer.org)
Furthermore, these three genes predicted overall survival and recurrence‑free survival in patients with ACC from the TCGA cohort. (cancerindex.org)
We show heat shock protein family D member 1 (HSPD1 or HSP60) as a survival gene ubiquitously expressed in NSCLC and associated with poor patients' prognosis. (biomedcentral.com)

Often multiple genes are involved in the given biological process as complex as the successful viral infection. (healthdrugpdf.com)

Low TS expression was predictive of improved OS of patients with negative EGFR-mutated advanced AC-NSCLC, whereas high ERCC1 expression resulted in poor OS in patients with positive EGFR-mutated advanced AC-NSCLC. (medsci.org)
Our data have provided evidence for a comparable prediction of clinical outcome in CMF-treated breast cancer patients using conventional clinical variables and gene expression based markers. (lu.se)
Bone Marrow Wilms Tumor Gene 1 Expression before Allogeneic Hematopoietic Cell Transplantation Correlates with Relapse in Acute Myeloid Leukemia Patients. (unito.it)

If one is starting from scratch, without any knowledge of what kind of gene it might be and what chromosome it resides on, the task is truly enormous. (healthdrugpdf.com)
This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. (cancerindex.org)
The identified DEGs included 20 downregulated genes and 51 upregulated genes, which were highly associated with the cell cycle, organelle fission, chromosome segregation, cell division and spindle stability. (cancerindex.org)

The -6 bp/-6 bp deletion polymorphism in the 3'UTR of TS is associated with decreased mRNA stability in vitro and lower intratumoral TS expression in vivo . (biomedcentral.com)
In addition, miR-101-3p decreased myeloid cell leukemia 1 ( MCL1 ) expression by binding to the 3'-untranslated region (3'-UTR) of its mRNA. (oncotarget.com)

Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
The analyzed gene datasets were downloaded from the Gene Expression Omnibus and The Cancer Genome Atlas (TCGA) database. (cancerindex.org)
Gene fitness was determined by bioinformatics analysis from available datasets from genetic screenings. (biomedcentral.com)

The gene is associated with allelic variants. (en-academic.com)

Indeed, a number of genes identified as potential manipulators of the host cell phenotype are absent from the T . equi genome. (biomedcentral.com)

We investigated the clinical significance of E2F1 gene expression in relation to its target genes, thymidylate synthase ( TS ) and Survivin , in case of non-small-cell lung cancer (NSCLC). (aacrjournals.org)
A large number of drug candidates have failed in clinical trials because of not only lack of efficacy but also non-verification of the predicted pharmacological mechanism of action due to insufficient interpretation of fundamental pharmacokinetic/pharmacodynamic principles, target engagement (TE), and expression of functional pharmacological activity 1 , 2 . (nature.com)

The top 14 hub genes were subsequently confirmed by reverse transcription‑quantitative polymerase chain reaction in ACC and adrenocortical adenoma samples. (cancerindex.org)

What pathways are this gene/protein implicaed in? (cancerindex.org)
This study aimed to investigate cisplatin (DDP)-susceptibility regulated by expression of the miRNAs and underlying pathways in GBC. (oncotarget.com)

However, most of the relevant studies have been somewhat controversial and the predictive accuracy of these genes is still limited ( 11 , 12 ). (iiarjournals.org)

Checkpoint kinase 2 (CHEK2), a cell cycle checkpoint regulator gene, codes for a kinase protein activated in response to radiation and other agents that cause breaks in the DNA. (termedia.pl)
VAN DER STRAETEN D., CASTAGNA A., RANIERA A. (2022) Foliar and root comparative metaboliomics and phenolic profiling of micro-tom tomato (Solanum lycopersicum L.) plants associated with a gene expression analysis in response to short daily UV treatments. (ugent.be)
Virally directed cytosine deaminase/5-fluorocytosine gene therapy enhances radiation response in human cancer xenografts. (uchicago.edu)

High TOP2A expression suggested significant relationship with worse cancer prognosis. (jcancer.org)

Moreover, cisplatin-resistant A549/DDP cells showed higher MALAT1 expression than cisplatin-sensitive lung cancer cells (A549, H460, H1299 and SPC-A1). (oncotarget.com)

Even if only those SNPs that are thought to lie within expressed genes are considered, their number is quite substantial. (healthdrugpdf.com)
It has been estimated that a gene of average size would contain about 15 SNPs. (healthdrugpdf.com)

XLS 97 kb) 12861_2017_153_MOESM8_ESM.xls (98K) GUID:?B2FE5241-0444-4D48-99D4-D5E55E4120EF Extra file 9: Desk S7: Target genes of the very best portrayed 18 miRNAs. (clinical-research-informatics.com)

Further exploratory ERCC1 and TS expression analyses were conducted to provide additional insights. (medsci.org)

Pemetrexed effectiveness has been related to the expression of its target thymidylate synthase. (eur.nl)

CHEK2 is not only a breast cancer susceptibility gene but also a multi-organ cancer susceptibility gene [15]. (termedia.pl)
Based on the androgen receptor (AR) expression, triple-negative breast cancer (TNBC) can be subdivided into AR-positive TNBC and AR-negative TNBC, also known as quadruple-negative breast cancer (QNBC). (biomedcentral.com)

They affect gene expression by regulating chromatin modification, transcription and post-transcriptional mechanisms [ 4 ]. (oncotarget.com)

FU is thought to kill cells via the inhibition of thymidylate synthase and also deprive cells of TTP during DNA replication, which leads to the introduction of uracil in DNA causing the fragmentation of newly synthesized DNA. (wikipedia.org)
Chronic myeloid leukemia cells resistant to interferon-alpha lack STAT1 expression. (unito.it)

Results: Ninety NSCLCs were divided into six subclasses by gene expression signatures. (eur.nl)

Experimental Biology Gene-nutrient interaction RIS Student ward. (rochester.edu)

Global expression profile-based classification has the potential to identify such tumor-intrinsic subclasses. (eur.nl)
Our analysis identified 50 genes that will be useful for definitive phylogenetic classification of T . equi and closely related organisms. (biomedcentral.com)

Quantitative reverse transcription-PCR was done to evaluate the gene expression of E2F1, TS , and Survivin . (aacrjournals.org)

There is thought to be more than 30,000 genes in the human genome. (healthdrugpdf.com)

Anatomy4

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0.00011

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