FragilisPrevotellaSpeciesBacterial contaminationMicrobial sourceBacteriaFusobacteriumIndicatorsAnaerobesIntestinalMycotoxin contaminationGenusMelaninogenicusDetectionMicroorganismsEscherichiaColiViral contaminationCross-contaminationQPCRAssaysMarkerPrimarilySewageSpecificityCommonlyResistanceWatersCanineSourceSurfacesAssessSpecificHumanEnvironmentSubstantialSourcesIncludeOccurrenceImportantSamples
Fragilis4
- One of the most important clinically is Bacteroides fragilis. (wikipedia.org)
- This is a scientifically proven instance where the bacterium Bacteroides fragilis prevents multiple sclerosis in mice. (kiyalongevity.com)
- Treated with Bacteroides fragilis , these rodents were able to overcome their core abnormalities, including communication deficit, which is the diagnostic symptom of autism. (kiyalongevity.com)
- Those microorganisms that are potential indicators of contamination in a dental environment include Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, Streptococcus mitis, Streptococcus salivarius, Corynebacterium diphtheriae, Bacteroides fragilis and Peptoestreptococus. (bvsalud.org)
Prevotella3
- 107 4.4.3 Prevotella-Bacteroides Detection. (taylorfrancis.com)
- Studies indicate that long-term diet is strongly associated with the gut microbiome composition-those who eat plenty of protein and animal fats have predominantly Bacteroides bacteria, while for those who consume more carbohydrates the Prevotella species dominate. (wikipedia.org)
- Bacteroides melaninogenicus has recently been reclassified and split into Prevotella melaninogenica and Prevotella intermedia. (wikipedia.org)
Species18
- Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. (wikipedia.org)
- Bacteroides species are normally mutualistic, making up the most substantial portion of the mammalian gastrointestinal microbiota, where they play a fundamental role in processing of complex molecules to simpler ones in the host intestine. (wikipedia.org)
- however, the main sources of energy for Bacteroides species in the gut are complex host-derived and plant glycans. (wikipedia.org)
- Bacteroides species also benefit their host by excluding potential pathogens from colonizing the gut. (wikipedia.org)
- Although Bacteroides species are anaerobic, they are transiently aerotolerant and thus can survive in the abdominal cavity. (wikipedia.org)
- In general, Bacteroides are resistant to a wide variety of antibiotics-β-lactams, aminoglycosides, and recently many species have acquired resistance to erythromycin and tetracycline. (wikipedia.org)
- This high level of antibiotic resistance has prompted concerns that Bacteroides species may become a reservoir for resistance in other, more highly pathogenic bacterial strains. (wikipedia.org)
- Some Bacteroides species are associated with Crohn's disease, appendicitis and inflammatory bowel disease. (wikipedia.org)
- Bacteroides species play multiple roles within the human gut microbiome. (wikipedia.org)
- Bacteroides species' main source of energy is fermentation of a wide range of sugar derivatives from plant material. (wikipedia.org)
- Aztreonam is a broad-spectrum antibiotic commonly used in plant tissue culture to control bacterial contamination from Gram-negative species. (toku-e.com)
- Cefoxitin and cefotetan have increasing rates of resistance in Bacteroides species and would not be used as monotherapy. (hopkinsguides.com)
- The results indicated 4,710,000 gene units of bovine bacteroides species. (mikecallicrate.com)
- Bacteroides species make up the majority of mammalian intestinal bacterial communities. (nwu.ac.za)
- To this end, we have been developing Bacteroides - and Lachnospiraceae-based species-specific assays that identify the presence of gene sequences uniquely found in these bacteria that are associated with their corresponding host species. (kennesaw.edu)
- We now have the ability to quantitatively assess the level of species-specific contamination in the environment using five MST tests: human, dog, bird, horse, and total animal. (kennesaw.edu)
- Here we report the disrupted transmission of maternal Bacteroides strains, and high-level colonization by opportunistic pathogens associated with the hospital environment (including Enterococcus , Enterobacter and Klebsiella species), in babies delivered by caesarean section. (nature.com)
- However, these tests do not exclude contamination of any other species. (riken.jp)
Bacterial contamination2
- The highest bacterial contamination was observed in the lids of the portable dark rooms. (bvsalud.org)
- DM is an impartial component for the deterioration of cirrhosis and is related to the incidence of foremost complications, which include ascites, renal insufficiency, hepatic encephalopathy , and bacterial contamination. (inutrimon.com)
Microbial source4
- therefore, microbial source tracking was implemented to determine various biological sources of contamination. (usgs.gov)
- The human-associated Bacteroides marker was the most frequently detected microbial source tracking marker in South Oyster Bay (50 percent positive detections). (usgs.gov)
- Recent large-scale sewage contamination of the Vaal River in South Africa is an example of where microbial source tracking (MST) by means of Bacteroides host-associated assays could be applied advantageously in mitigation efforts. (nwu.ac.za)
- Microbial source tracking (MST) attempts to identify specific animal sources of fecal contamination in the environment. (kennesaw.edu)
Bacteria10
- However, fecal indicator bacteria are not specific to human fecal contamination ( 6 ) and have a poor correlation with exposure risk to enteric viruses ( 4 , 7 - 9 ). (cdc.gov)
- Various bacteria of the phylum Bacteroidetes have been proposed as the primary hosts of crAssphage, which was supported by recent findings that phage ΦCrAss001 from human feces could be isolated in Bacteroides ( 20 ). (cdc.gov)
- Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. (wikipedia.org)
- An alternative fecal indicator organism, Bacteroides, has been suggested because they make up a significant portion of the fecal bacterial population, have a high degree of host specificity that reflects differences in the digestive system of the host animal Over the past decade, real-time polymerase chain reaction (PCR) methods have been used to detect the presence of various microbial pathogens through the amplification of specific DNA sequences without culturing bacteria. (wikipedia.org)
- This technique allows quantification of genetic markers that are specific to the host of the bacteria Bacteroides and allow detection of recent contamination. (wikipedia.org)
- Water samples are routinely collected by the New York State Department of Environmental Conservation in the bay and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. (usgs.gov)
- Unlike conventionally applied faecal indicator bacteria (FIB), Bacteroides has very limited survival capabilities in the environment due to their anaerobic nature. (nwu.ac.za)
- Bacteroidetes 0 domande A phylum of bacteria comprised of three classes: Bacteroides, Flavobacteria, and Sphingobacteria. (lookformedical.com)
- The bacteria infect the foal via ingestion through the mouth, with the mare often being the source of contamination. (eqlifemag.com.au)
- Other bacteria associated with diarrhoea in foals include Bacteroides, Campylobacter, and Leptospira spp. (eqlifemag.com.au)
Fusobacterium1
- Proteobacteria and Actinobacteria were the most common contaminants, whereas Fusobacterium and Bacteroides were commonly found in colorectal cancer tissue samples. (microbiomepost.com)
Indicators4
- are widely used as indicators to assess the presence of human fecal contamination of environmental waters ( 2 - 5 ). (cdc.gov)
- Molecular-based approaches like quantitative polymerase chain reaction (qPCR) assays targeting genetic markers for 16S rRNA genes of fecal indicators can be used to overcome the disadvantages of using culture-dependent methods to evaluate fecal contamination. (biomedcentral.com)
- Meanwhile, data continue to accumulate to ensure the suitability of coliphages as indicators of viral contamination: 1) their abundance in different local and geographic environments, and 2) their behavior against disinfection and natural inactivation. (bluephage.com)
- This led to the acceptance of somatic coliphages as indicators of fecal/viral contamination of waters. (bluephage.com)
Anaerobes1
- Because anaerobes are present on skin and mucous membranes, even minimal contamination with normal florae can be misleading. (medscape.com)
Intestinal4
- In cases where Bacteroides can move outside the gut due to gastrointestinal tract rupture or intestinal surgery, Bacteroides can infect several parts of the human body. (wikipedia.org)
- Bacteroides are typical components of intestinal, oral and vaginal flora. (hopkinsguides.com)
- In hepatitis B cirrhosis, the variety of intestinal microbiota decreased, and the boom within the abundance of Escherichia coli and Veillonella undoubtedly correlated with the Child-Turcotte-Pugh (CTP) score, at the same time as the lower within the abundance of Bacteroides negatively correlated with the CTP score. (inutrimon.com)
- microbiota intestinal, en especial con el uso de Lactobacilli y Bifidobacterium spp. (bvsalud.org)
Mycotoxin contamination1
- As mycotoxin contamination is a critical factor that destabilizes global food safety, the current review provides an updated overview of the (co-)occurrence of A. flavus and F. verticillioides and (co-)contamination of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) in maize. (usda.gov)
Genus3
- Bacteroides ovatus , a member of the genus Bacteroides , is considered for use in molecular-based methods as a general fecal indicator. (biomedcentral.com)
- Among the genus Bacteroides , Bacteroides ovatus is considered for use as a general fecal contamination indicator [ 10 ]. (biomedcentral.com)
- We have been developing a MST system that detects DNA biomarkers from the gram negative bacterial genus Bacteroides and the family Lachnospiraceae. (kennesaw.edu)
Melaninogenicus1
- In 1983, Pingleton and Jeter reported extensive thoracic wall gangrene with Bacteroides melaninogenicus and Viridans streptococci after minimal pleurotomy for empyema. (spandidos-publications.com)
Detection3
- First, most conventional FIBs can proliferate under natural conditions after being released into the environment, and the detection of these FIBs may overestimate the original contamination level. (biomedcentral.com)
- Therefore, detection of dog or canine fecal contamination in aquatic environments is important for mitigating risks. (qld.gov.au)
- Subsequently by detection of the host source and quantification of marker levels the extent of contamination as well as possible points of entry into an area of interest may be determined. (nwu.ac.za)
Microorganisms1
- Peritonitis can also result from injury, contamination with microorganisms, chemicals or both. (wikidoc.org)
Escherichia1
- Last month, the United States Food and Drug Administration (FDA) announced that a California company was recalling hundreds of cases of packaged salads due to fears of possible Escherichia coli O157:H7 contamination. (emsl.com)
Coli3
- Unlike E. coli or Enteroccoccci , host-specific strains of Bacteroides and Lachnospiraceae exist. (kennesaw.edu)
- Parallèlement, l'ARNr16S de Bacteroides d'origine humaine (HF183) et des ruminants (Rum2Bac) ainsi que des analyses microbiologiques conventionnelles (E.coli fécaux, entérocoques) et physicochimiques (pH, température, COT, pluviometrie) ont été réalisées. (univ-lorraine.fr)
- En zone rurale, la présence des STEC O26, O103, O145 et O157 viable corrèle positivement avec une augmentation génomique ciblant E. coli mais aussi les marqueurs de contamination fécale d'origine humaine. (univ-lorraine.fr)
Viral contamination1
- Recently, both norovirus and adenovirus have been suggested as potential biomarkers of viral contamination to assess hygiene status and potential human health risk of contaminated surfaces and hands of affected persons ( 4 , 12 , 14 - 17 ). (cdc.gov)
Cross-contamination3
- Change the gloves frequently between samples if you are collecting many samples to prevent accidental cross-contamination. (emsl.com)
- In conclusion, the present study highlights the need to establish strategies to prevent bacterial cross-contamination during radiographic procedures in dental settings. (bvsalud.org)
- Accordingly, the propagation of infectious diseases is made possible via cross-contamination 1 . (bvsalud.org)
QPCR2
- One study has measured the amount of Bacteroides by using qPCR to quantify the host-specific 16S rRNA genetic marker. (wikipedia.org)
- A combination of qPCR and propidium monoazide (PMA) pretreatment has been developed for the Bacteroides spp. (biomedcentral.com)
Assays4
- Unfortunately, as with any developing technique, increased popularity and frequency of application have brought several shortcomings of Bacteroides related assays to light. (nwu.ac.za)
- A systematic literature review was performed identifying recurring themes with regard to challenges and limitations faced by researchers applying Bacteroides related assays to environmental water samples. (nwu.ac.za)
- These methods are best applied in a toolbox approach along with several other assays or markers in order to estimate the full extent of contamination in a sampling area. (nwu.ac.za)
- Standardisation of Bacteroides host-associated assays is crucial for successful application of these techniques, especially with regards to comparability of data. (nwu.ac.za)
Marker2
- All detections of the canine-associated Bacteroides marker were in samples collected during the winter season and were associated with fecal coliform concentrations below the reporting limit, indicating that birds are not a persistent source of fecal coliform to South Oyster Bay. (usgs.gov)
- The DF418 marker gene was detected in all sewage and animal feces and deemed not suitable for canine fecal contamination tracking in sub-tropical Australia. (qld.gov.au)
Primarily1
- primarily Bacteroides phages and specific sero/genogroups of F-specific RNA coliphages. (bluephage.com)
Sewage2
- Culture methods are available to test for sewage contamination in buildings (M117 & M013) as well as new Bacteroides PCR based methods. (emsl.com)
- A lack of understanding the implications of sewage contamination in water combined with inadequate municipal management practices contribute toward deterioration of water infrastructure. (nwu.ac.za)
Specificity1
- While traditional fecal coliform enumeration can give a general measure of contamination, the specificity imparted by modern MST provides a more informative assessment of contaminants and their likelihood to affect human health outcomes. (kennesaw.edu)
Commonly1
- As such, the culture-dependent method to enumerate conventional FIBs is commonly used for detecting fecal contamination. (biomedcentral.com)
Resistance1
- On the only hand, liver sicknesses associated with HBV contamination can insult blood glucose metabolism and result in insulin resistance through diverse mechanisms. (inutrimon.com)
Waters1
- Both bacterial groups cannot survive long periods outside of their hosts and are considered a good indicator of recent fecal contamination in environmental waters. (kennesaw.edu)
Canine2
- Canine contamination was prolific at source sites but was associated with low fecal coliform concentrations in the winter months. (usgs.gov)
- Canine fecal contamination was infrequently detected in environmental water samples. (qld.gov.au)
Source5
- Thus, monitoring of human fecal contamination and identifying the source of contamination is an important approach to prevent transmission of gastroenteritis viruses for which humans are the only natural host (e.g., human norovirus) ( 1 ). (cdc.gov)
- Water can become a major source of contamination in both outdoor and indoor pig producing systems. (thepigsite.com)
- Analysis revealed that the most substantial source of fecal contamination to South Oyster Bay was stormwater, particularly during the summer months. (usgs.gov)
- Animal fecal contamination in aquatic environments is a major source of zoonotic diseases in humans. (qld.gov.au)
- Are septic systems the source of human fecal contamination in private wells in rural Pennsylvania? (google.ca)
Surfaces2
- To validate the potential use of crAssphage for detecting human fecal contamination on environmental surfaces and hands, we tested stool samples (n = 60), hand samples (n = 30), and environmental swab samples (n = 201) from 17 norovirus outbreaks for crAssphage by real-time PCR. (cdc.gov)
- Our data suggest that crAssphage could be used to detect human fecal contamination on environmental surfaces and hands. (cdc.gov)
Assess1
- The U.S. Geological Survey worked in cooperation with the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering South Oyster Bay, a shallow embayment on the southern shore of Long Island, New York. (usgs.gov)
Specific1
- Bacteroides is specific for fecal contamination from all sources-animals, birds, and human. (emsl.com)
Human2
- Using Total Bacteroides (M095), it is now possible to determine the amount of fecal contamination from non-human and human sources. (emsl.com)
- Using Human Bacteroides (M199) determines the amount of fecal contamination from human sources only. (emsl.com)
Environment1
- Because they are anaerobic, Bacteroides will not multiply in the environment. (emsl.com)
Substantial1
- Based a classification scheme developed to convey the degree of fecal contamination to stakeholders and resource managers, the Cedar Creek near Bay Place and Unqua Lake Culvert sites were identified as locations that contribute substantial fecal contamination to South Oyster Bay. (usgs.gov)
Sources2
- Nombreuses sont les sources ponctuelles ou diffuses de contamination et de dissémination environnementale de cette pollution biologique fécale, tant dans les zones urbaines qu'agricoles. (univ-lorraine.fr)
- These sites, depending on the frequency of use, could be significant sources of surface and groundwater contamination. (shengsci.com)
Include1
- These include tail biting, ear nibbling, greasy pig disease, trauma to the gums following teeth removal, necrosis of knees in piglets, joint infections, eperythrozoonosis (needle contamination) and of course fighting. (thepigsite.com)
Occurrence2
- This study provides a mechanistic understanding to some of the important environmental relevant factors that influenced the inactivation kinetics of B. ovatus in the presence of sunlight irradiation, and would facilitate the use of B. ovatus to indicate the occurrence of fecal contamination. (biomedcentral.com)
- Les analyses en composantes principales révèlent une dynamique/occurrence différente selon les sites et impactées par les différents paramètres analysés. (univ-lorraine.fr)
Important1
- For sampling Bacteroides it is important to wear sterile nitrile gloves. (emsl.com)
Samples3
- A larger sample size is better in the event the Bacteroides concentration in these samples is very dilute. (emsl.com)
- 10. ALWAYS take background samples in non-fecally contaminated areas to determine if there is background contamination from an unidentified event that may bias your results. (emsl.com)
- What's more, studies that analyze the microbiota of samples with a low prevalence of bacteia suffer from contamination during sample collection and DNA extraction. (microbiomepost.com)