Protein StabilityProtein ConformationModels, MolecularEnzyme StabilityDrug StabilityThermodynamicsProtein DenaturationProtein FoldingProtein Structure, TertiaryProtein Structure, SecondaryProtein BindingMolecular Sequence DataCircular DichroismAmino Acid SequenceMutationBinding SitesKineticsTemperatureCrystallography, X-RayProteinsSpectrometry, FluorescenceMolecular ConformationMutagenesis, Site-DirectedRNA StabilityAmino Acid SubstitutionGuanidineModels, ChemicalHydrogen-Ion ConcentrationHydrogen BondingStructure-Activity RelationshipMolecular Dynamics SimulationEscherichia coliProtein UnfoldingUbiquitinationProteolysisMagnetic Resonance SpectroscopyProtein EngineeringRecombinant ProteinsProteasome Endopeptidase ComplexNuclear Magnetic Resonance, BiomolecularComputer SimulationSolventsUbiquitinCell LineLigandsCalorimetry, Differential ScanningNucleic Acid ConformationProtein Structure, QuaternaryStatic ElectricityBase SequenceHot TemperatureSolutionsDimerizationUreaBacterial ProteinsMutant ProteinsHydrophobic and Hydrophilic InteractionsMuramidaseTryptophanProtein MultimerizationCatalytic DomainAmino Acid MotifsPeptidesUbiquitin-Protein LigasesCysteinePhosphorylationHEK293 CellsSpectrophotometry, UltravioletEntropyModels, BiologicalProtein Processing, Post-TranslationalSequence Homology, Amino AcidCalorimetryDisulfidesCatalysisMutation, MissenseWaterDNASequence AlignmentRecombinant Fusion ProteinsHalf-LifeApoproteinsMolecular StructurePoint MutationTransition TemperatureFluorescenceSubstrate SpecificityRNA, MessengerEscherichia coli ProteinsHydrolysisLysineDNA-Binding ProteinsAmino AcidsPeptide FragmentsAnilino NaphthalenesulfonatesSignal TransductionBiophysicsCell Line, TumorSaltsTime Factors