• The sensitivity and dynamic range of the QIAseq Library Quant System were compared against Agilent's High Sensitivity DNA Kit, using two NGS libraries of differing concentrations. (qiagen.com)
  • By contrast, the QIAseq Library Quant Array was able to quantify both libraries (see figure The QIAseq Library Quant System enables quantification of libraries with concentrations below the detection limit of conventional methods The QIAseq Library Quant Array's high sensitivity and broad dynamic range enable the quantification of both the NGS-L1 and NGS-L2 libraries [A]. By contrast, Agilent's High Sensitivity DNA Kit (for use with the Agilent 2100 BioAnalyzer) quantified only the NGS-L1 library; with this kit, the NGS-L2 concentration was too low for quantification [B]. "> QIAseq Library Quant System enables quantification of libraries with concentrations below the detection limit of conventional methods ). (qiagen.com)
  • The high sensitivity of real-time PCR allows quantification of libraries at very low concentrations, even below the detection threshold of conventional spectrophotometric methods. (qiagen.com)
  • The QIAseq Library Quant System is available in both a tube and an array format, and is optimized with QIAseq qPCR SYBR ® Green Mastermixes to provide superior sensitivity and a wide dynamic range. (qiagen.com)
  • In summary, 1) the assay standard curves and the relative signals of the calibrator concentrations were consistently within the criteria specified by the manufacturer, 2) the obtained assay sensitivity was calculated to be 4.33 pg/mL while the manufacturer claimed sensitivity was 6.15 pg/mL, 3) two samples with very high and high Klotho concentrations were used at different dilution to evaluate the assay linearity. (cdc.gov)
  • There are a number of factors that can be altered to obtain optimum assay performance and thereby lead to higher molecular sensitivity, specificity and precision. (sigmaaldrich.com)
  • Whenever primer dimer products are produced and amplified, they divert reaction components away from synthesis of the desired product, thereby reducing assay efficiency and sensitivity. (sigmaaldrich.com)
  • Sensitivity of an assay dictates the detection of the target within the dynamic range of the assay with the caveat that the lower limit of detection could vary marginally from batch to batch. (genengnews.com)
  • Technologically, luminescent and fluorescent modes offer greater sensitivity compared to absorbance assays. (genengnews.com)
  • Both assay format and detection technology play major roles in sensitivity. (genengnews.com)
  • P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
  • The assays were evaluated for sensitivity and specificity. (cdc.gov)
  • JE2) for their sensitivity and specificity detection of the bacterium in water and soil by a modification of PCR assay. (thescipub.com)
  • However, numerous other clinical applications warrant the use of estradiol assays with extra sensitivity, simultaneous estrone testing, or both. (medscape.com)
  • the other two use the Elecsys platform or an enzyme-linked immunosorbent assay (ELISA). (alzforum.org)
  • Next was the N4PE Simoa (brown) and Euroimmun ELISA immunoassay (gray), followed by a U Gothenburg IP-MS (green) and Quanterix Simoa (red) assays. (alzforum.org)
  • Prior to the start of the study, an extensive validation of the IBL ELISA method for measurement of Klotho concentration in human samples was performed and the results of the Validation provided to the study investigators. (cdc.gov)
  • Two quality control samples with low and high concentration of Klotho were also analyzed in duplicate in each ELISA plate. (cdc.gov)
  • Applications Tested: The Functional Grade Purified MP5-20F3 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-6 bioactivity. (thermofisher.com)
  • A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 µg/mL. (thermofisher.com)
  • A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8-1000 pg/mL should be included in each ELISA plate. (thermofisher.com)
  • MBS2600477 is a ready-to-use microwell plate ELISA (enzyme-linked immunosorbent assay) kit for testing biological samples for the presence of specific anti-dsDNA IgG ELISA kit target analytes. (cellgenetherapy.com)
  • The TL1A soluble (human), ELISA kit is a complete assay for the quantitative determination of soluble human TL1A concentration. (enzolifesciences.com)
  • Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
  • Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
  • These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
  • Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
  • These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
  • E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
  • ELISA has gained widespread acceptance across a very diverse range of fields (eg diagnosis of infectious diseases, food allergen detection, plant pathogens to biomarkers), but it never achieved significant adoption by early drug discovery labs. (ddw-online.com)
  • The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
  • This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
  • This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
  • The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
  • The Monkey Albumin ELISA Assay is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Albumin in biological fluid of Monkey. (eaglebio.com)
  • This ELISA kit can be used to measure albumin in serum, tissue extracts and other biological fluids. (eaglebio.com)
  • Eagle Biosciences has quickly become a leading provider of ELISA assay kits, HPLC assay kits, Molecular Biology assays, antibodies, and proteins. (eaglebio.com)
  • An indirect ELISA and a real time immuno-PCR (RT-iPCR) assay for the timely and sensitive detection of A. euteiches were developed using antiserum specific to oospores of A. euteiches isolates. (uleth.ca)
  • RT-iPCR was 100 times more sensitive over the linear working range than indirect ELISA. (uleth.ca)
  • Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. (illinois.edu)
  • Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). (eaglebio.com)
  • In environmental toxicology, effects range low (ERL) and effects range median (ERM) are measures of toxicity in marine sediment. (wikipedia.org)
  • They are derived from biological toxicity assays and synoptic sampling. (wikipedia.org)
  • NOAA originally calculated ERL/ERMs using existing toxicity data compiled from completed toxicity assays with varying endpoints, including effects on commonly tested organisms, particularly at sensitive life stages. (wikipedia.org)
  • Using data already collected ("data mining") has the advantage of being able to quickly and inexpensively make an assessment with a large dataset that would otherwise require much more time-consuming and costly specific toxicity assays. (wikipedia.org)
  • In summary, the key links between the compiled studies are the testing of a specific analyte - toxicity assays used are for sediment, and a significant effect must be determined. (wikipedia.org)
  • We combined this with toxicity assay data from the US Environmental Protection Agency's (EPA's) Toxicity Forecaster (ToxCast). (cdc.gov)
  • In certain conditions, the free drug concentration may be significantly higher than expected from the measured total concentration, and consequently toxicity may occur (Dasgupta, 2002). (vin.com)
  • With respect to toxicokinetics, all MDI substances are highly reactive and exhibit strong reactivity with biological nucleophiles which are directly related to the toxicity mode of action. (europa.eu)
  • The first library was quantifiable by both systems, but the low concentration of the second library was below the detection limit of Agilent's 2100 BioAnalyzer. (qiagen.com)
  • The array format provides this standard in five predispensed, sequential 10-fold dilutions mixed with a PCR primer assay in triplicate, ensuring that your sample library will fall within the detection range of the array (see figure Principle of the QIAseq Library Quant System The serial dilutions of the DNA standard (5 sequential 10-fold dilutions) generate a standard curve. The sample library should fall within the standard curve. "> Principle of QIAseq Library Quant System ). (qiagen.com)
  • The concentration gradients of the kit standards or positive controls provide a theoretical detection range of the kit in biological research samples containing specific anti-dsDNA IgG. (cellgenetherapy.com)
  • With dye-based detection such as SYBR Green I, primer dimers also affect assay specificity because the nonspecific DNA-binding dye will bind to the primers and be detected along with the desired product. (sigmaaldrich.com)
  • The dynamic range of an assay defines both the lower and upper limits of detection. (genengnews.com)
  • Therefore, the accurate detection of physiological NO concentration is crucial to the understanding of NO signaling and its biological role. (intechopen.com)
  • Most methods used for NO detection are indirect including spectroscopic approaches such as the Griess assay for nitrite and detection of methemoglobin after NO reaction with oxyhemoglobin. (intechopen.com)
  • The detection and evaluation of concentration of influenza virus proteins in biological samples is critical in a broad range of medical and biological investigations regarding the concern over potential outbreaks of virulent influenza strains in animals and humans. (omicsonline.org)
  • Epidemiological and ecological studies on pathogen distribution in the environment are seriously hindered by the lack of efficient detection method especially when the concentration of the pathogen is either very low or is present in a latent, dormant or non-culturable state. (thescipub.com)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • This study establishes a new link between actual human internal POP levels and the endocrine active dose in vitro, applying partitioning-controlled dosing from silicone to the H295R steroidogenesis assay: (1) Measured concentrations of POPs in silicone breast implants were taken from a recent study and silicone disks were loaded according to these measurements. (nih.gov)
  • Methodologically, the study showed that silicone can be used as reference partitioning phase to transfer in vivo exposure in humans (silicone implants) to in vitro assays (partition-controlled dosing). (nih.gov)
  • that is, it needs to be performed in relevant in vitro systems with pharmacologically relevant concentrations of components to yield biologically relevant data. (genengnews.com)
  • Scientists at BellBrook and/or the instrument vendor have tested Transcreener reagents with each instrument to determine the optimal filters and settings for maximal assay performance. (bellbrooklabs.com)
  • Claims relating to the performance of assays should be verified under the conditions of the study, including test samples, specific reagents and the instrument of choice. (sigmaaldrich.com)
  • However, all assays have a set of optimal conditions and these are dependent on the instrument, selected reagents (buffer conditions), primer concentration and/or annealing temperature (T a ), magnesium concentration and even ramp rate. (sigmaaldrich.com)
  • Find or custom design the right target-specific assays and panels to research your biological targets of interest. (qiagen.com)
  • Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)
  • Similarly, when multiple targets are to be detected simultaneously in multiplex reactions, assay conditions must be optimized to detect all targets equally. (sigmaaldrich.com)
  • Additionally, for complex biological matrices, antibody selection can be challenging since protein targets secreted from cells into cell culture media or serum may be cleaved or modified, altering epitope recognition-hence the benefit of using protease cocktail inhibitors. (genengnews.com)
  • The hybridization kinetics of the hybrid sensor was studied with synthetic and AluI digested real pork targets from moderate to extreme target concentrations and a sigmoidal relationship was found. (hindawi.com)
  • Methods: We queried the National Health and Nutrition Examination Study (NHANES) from 1999-2014 for pesticide exposure biomarker concentrations among farm workers and non- farm workers by citizenship status. (cdc.gov)
  • Non-U.S. citizens were 1.39 times more likely to have bioactive pesticide biomarker concentrations compared to people with U.S. citizenship (adjusted OR 1.39, 95% CI: 1.17, 1.64). (cdc.gov)
  • Inter-assay and intra- about 4.4 million people, had impaired disorders such as hypo- or hyperthy- assay variations for PTH were 8.9% and fasting glucose [2,3]. (who.int)
  • This kit is to be used with biological fluids (serum and cell culture supernatant) from human origin. (enzolifesciences.com)
  • For the quantitative determination of soluble human TL1A from biological fluids (serum and cell culture supernatant). (enzolifesciences.com)
  • Therapeutic drug monitoring (TDM) refers to the quantitative measurement of drugs in serum/plasma (or other biological fluids). (vin.com)
  • Buprenorphine and norbuprenorphine were extracted from biological fluids by solid-phase extraction. (johnshopkins.edu)
  • DCM is very volatile and thus sampling and storage conditions of biological fluids are important. (who.int)
  • thus, the absorbance, at 450 nm, is a measure of the concentration of Alb in the test sample. (eaglebio.com)
  • Effects of physiological variables (e.g., age) and concomitant disease (e.g., renal failure) on serum/plasma concentrations are not well documented in small animal medicine, which may lead to misinterpretation of the observed concentrations. (vin.com)
  • Buprenorphine plasma concentrations ranged from less than 0.20 ng/mL to 8.7 ng/mL. (johnshopkins.edu)
  • Cytokines are small signaling proteins that are present in serum and cell secretomes at concentrations in the pg/mL or ng/mL range. (illinois.edu)
  • Lipid research encompasses the large-scale profiling of cellular lipids, the quantification of specific lipids in biological samples and the documentation of the interactions between lipids and other lipids, proteins and metabolites within the body. (sanbio.nl)
  • To facilitate the accurate quantification of total bile acids in serum and plasma, the 5th Generation Total Bile Acids Assay has emerged as a reliable and advanced diagnostic tool. (randox.com)
  • The final antibody concentration in the reaction is 4 nM in a 20 µL final reaction volume. (bellbrooklabs.com)
  • The MP5-20F3 antibody at 0.03 µg/mL has been found to inhibit by 50% the biological effects of 0.01 ng/mL mouse IL-6 in an B9 cell proliferation assay. (thermofisher.com)
  • Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay. (thermofisher.com)
  • The assay produces an excellent signal at low substrate conversions for a broad range of initial substrate concentrations (0.1-1,000 µM). (bellbrooklabs.com)
  • In a drug discovery process, compounds are usually tested using automated screening to quickly assess their biological activities,18,19 which requires a protocol to achieve the chemoselective and efficient synthesis of screening substances in situ in multi well plates with low substrate concentrations and little volumes. (baxkyardgardener.com)
  • SAGIAN Automated Assay Optimization (AAO) software is now allowing scientists to import designed experiments that are translated into corresponding Biomek FX methods. (qualitydigest.com)
  • Assay optimization and validation are essential, even when using assays that have been predesigned and commercially obtained. (sigmaaldrich.com)
  • Optimization is required to ensure that the assay is as sensitive as is required and that it is specific to the target of interest. (sigmaaldrich.com)
  • Assays requiring high specificity are particularly vulnerable when performed without optimization and adequate controls. (sigmaaldrich.com)
  • Since it is usual to run the assay on a selected instrument and under standard buffer conditions, most optimization procedures are focussed on modification of primer binding kinetics using primer concentration or annealing temperature (T a )/melting temperature (T m ). (sigmaaldrich.com)
  • Optimization of experimental assay design is crucial for the overall success of any drug discovery initiative. (genengnews.com)
  • From this, Zetterberg concluded that the assays needed to improve before they were ready for standardization, and for the subsequent applied research that moves tests beyond research-grade toward clinical chemistry certification and from there to doctors' offices around the world. (alzforum.org)
  • Drug dosage adjustments based on the assumption that measured drug concentrations are reliable predictors of clinical response may be risky. (vin.com)
  • The drug concentration must be always interpreted in light of the clinical response to the treatment, the characteristics (age, body weight, breed, species. (vin.com)
  • Development of such assays is relatively expensive as their validity should be demonstrated through clinical trials. (vin.com)
  • Clinical trial--derived pharmacokinetic information may be extremely useful to define therapeutic ranges of concentration. (vin.com)
  • Well-defined reference ranges are at the heart of clinical practice and, without them, clinicians can make erroneous diagnoses that could lead to patients receiving costly, lifelong treatments that they don't need or deny treatments to those who need them," said Dr Bhasin said in an Endocrine Society press release. (medscape.com)
  • As each study had used different assays for measuring testosterone, with each assay using different calibrators, the team obtained fasting morning serum samples from 100 men in each cohort, which were then analyzed in the CDC Clinical Reference Laboratory using a single, highly calibrated assay. (medscape.com)
  • After US Food and Drug Administration approval in 2019, some US clinicians rapidly implemented BPaL using an initial 600-mg linezolid dose adjusted by serum drug concentrations and clinical monitoring. (cdc.gov)
  • The information lost in OFAT designs may significantly reduce assay quality and robustness. (qualitydigest.com)
  • This process has already been undertaken we have examined critically the relevance to several years ago for the local lymph node assay humans, the robustness and the reliability of (LLNA) (2). (cdc.gov)
  • This dissertation describes the fabrication, characterization, development, and application of silicon photonic microring resonator technology to multiplexed protein measurements in a variety of biological systems. (illinois.edu)
  • Itdetected PB1-F2 with the linear range extended from 10 to 500 nM, repeatability of 5% for 500 nM PB1-F2 and showed saturationof protein concentrations higher than 1 μM. (omicsonline.org)
  • However, although Michael acceptors are reactive, the Michael addition is reversible and it can be assumed that also other parts of the molecules will moderate the binding to and the release from any given nucleophilic site in a protein, and thereby moderate a specific biological activity. (lu.se)
  • The substrates were characterized by scanning electron microscopy and their plasmonic sensitivities were evaluated using sucrose solutions with different concentrations. (scielo.br)
  • Ideally, substances stated in such methods should be put through biological assays straight without additional purification. (baxkyardgardener.com)
  • However, with the partitioning-controlled freely dissolved concentrations (Cfree) as dose metrics, dose-response curves were left-shifted by two orders of magnitude relative to spiked concentrations. (nih.gov)
  • Transcreener assays are not validated with cell lysates, blood, serum, or other biological samples. (bellbrooklabs.com)
  • The numerical values are incorporated in sediment quality guidelines (SQGs) that were developed by Long and Morgan for the National Oceanic and Atmospheric Administration (NOAA) National Status & Trends program as informal tools to evaluate whether a concentration of a contaminant in sediment might have toxicological effects. (wikipedia.org)
  • NOAA scientists use SQGs as a way to estimate if a concentration of contaminant in a sediment sample may have toxicological effects. (wikipedia.org)
  • We find that farm workers and those without US citizenship are significantly more likely to be exposed to concentrations of pesticides which are bioactive in toxicological assays. (cdc.gov)
  • In this investigation, the cytotoxicity of 20 α-methylene-γ-lactones towards normal breast epithelial MCF-10A cells as well as breast cancer JIMT-1 cells is compared, by determining the inhibitory concentration 50 (IC50) from dose response curves. (lu.se)
  • The results show that the field has reduced variation within each assay to create better tests," co-author Henrik Zetterberg, University of Gothenburg, Sweden, told Alzforum. (alzforum.org)
  • In biological assays leaves extract showed a MIC (Minimum Inhibitory Concentration) variation from 6.2 to 53.7% for S. aureus and S. sanguinis bacteria. (ufrr.br)
  • While highlighting that the findings establish a reference range for testosterone, he added: "These data also show that variations in assays are an important contributor to variation in testosterone levels in cohorts from different geographic regions. (medscape.com)
  • Age and assay variation values for 25(OH)D from the third national Surveil ance of sex were matched between the 2 groups. (who.int)
  • Total suspended solids in the settled sludge were adjusted to a nominal concentration of approximately 4000 mg/L by dilution with municipal water. (europa.eu)
  • The array format workflow (see figure QIAseq Library Quant Array workflow Each sample DNA library is combined with QIAseq qPCR SYBR ® Green Mastermix, aliquoted in technical triplicate across the QIAseq Library Quant Array, and analyzed with qPCR. Raw C T values are copied into the data analysis Excel template, and the software automatically calculates the library concentration or dilution factor. "> QIAseq Library Quant Array workflow ) begins with two tenfold dilutions of the sample library, to ensure that its concentration falls within the range of the serially diluted standards. (qiagen.com)
  • Studies are screened, and only those assays using standardized methods and resulting in significant effects are used for the determination of ERL/ERM guidelines. (wikipedia.org)
  • BL cells and normal fibroblasts were exposed to CDBPA for cytotoxicity screening by XTT assay, followed by cell cycle arrest, determination of apoptosis using Flow Cytometry and by annexin V/FITC/PI assay. (longdom.org)
  • Determination of Klotho concentrations was performed on frozen NHANES 2011-2012 samples that were received and tested during the period 2019-2020. (cdc.gov)
  • IL-1 and IL-6 were significantly higher in the study groups, the first presentation and relapse groups having the highest concentrations. (who.int)
  • Serum TNF concentration and urinary -2-m excretion were significantly higher in the first presentation and relapse groups. (who.int)
  • We will characterize the DOM pool and its molecular weight fractions and determine the trace metal concentration (Fe, Cu, Cd, Mn) and speciation in the different DOM molecular weight fractions. (europa.eu)
  • Lipid analysis can be challenging due to the sheer number of different cellular lipids, which can range from tens to hundreds of thousands, and their varied concentration in samples. (sanbio.nl)
  • The factors that need to be considered include: selection of the most appropriate assay technologies, reading modalities, and experimental models (biochemical, cellular, or animal). (genengnews.com)
  • Phenotypic cellular assay models more accurately reflect the complex biology of disease, allows for multiparametric measurement of ex vivo or in vivo events, and enables the characterization of intractable target(s). (genengnews.com)
  • Biochemical assays are simpler to execute than their cellular counterparts and offer better consistency. (genengnews.com)
  • Nitric oxide (NO) is an important signaling molecule that regulates a diverse range of physiological and cellular processes in many tissues. (intechopen.com)
  • L. egeregia was regarded as the prototype of the anticancer species due to its profound flavonoid concentration (85.40 µg/mL) and cytotoxicity (9.46 µg/mL) compared to other extracts. (who.int)
  • An assay that has been designed such that all desirable design criteria were met ( PCR/qPCR/dPCR Assay Design ) is likely to perform well under a wide range of conditions. (sigmaaldrich.com)
  • The primer design can be reviewed with respect to the assay design guidance provided in PCR/qPCR/dPCR Assay Design . (sigmaaldrich.com)
  • The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences to address a wide ranging biological problem in food industry, diagnostic laboratories and forensic medicine. (hindawi.com)
  • Compartmentalization is a ubiquitous building principle in cells, which permits segregation of biological elements and reactions. (nature.com)
  • Harmonized reference normal ranges for testosterone levels in healthy, nonobese men have been generated in a landmark study that should aid in accurately diagnosing hypogonadism , say US investigators. (medscape.com)
  • However, the task is made more difficult by a lack of both defined references ranges for testosterone and standardized assays. (medscape.com)
  • Harmonizing testosterone levels from four major cohort studies, Shalender Bhasin, MD, from Brigham and Women's Hospital, Harvard Medical School in Boston, Massachusetts, and colleagues were able to identify a reference range for testosterone that could help clinicians in decision making and improve patient care - this was in the region of 250 to 900 ng/dL for 19- to 39-year-old men. (medscape.com)
  • The new findings, which are based on measurements taken from over 9000 men and calibrated against a US Centers for Disease Control and Prevention (CDC) standardized assay, offer, for the first time, a reliable baseline for total testosterone levels. (medscape.com)
  • He emphasized that it is important that the new reference range for testosterone be taken "into consideration in the tests that clinicians and patients depend on for accurate diagnoses. (medscape.com)
  • As testosterone levels decline with age, the researchers initially generated reference ranges in healthy, nonobese men aged 19 to 39 years, followed by ranges stratified by decade of age. (medscape.com)
  • 1,2 Utilizing samples collected from trained belugas ( Delphinapterus leucas ), the aims of this project were to validate an assay for progesterone in beluga blow, determine if progesterone concentrations in blow correlated with those in matching blood samples, and to evaluate the ability to assess the pregnancy status and ovarian function of the beluga using blow progesterone concentrations. (vin.com)
  • Chromatin immunoprecipitation assay was performed to evaluate the histone modification of gene loci that are regulated by lunasin and cytokine. (weeksmd.com)
  • The newly created designs evaluate main effects and interactions between experimental factors such as sample concentration, reagent types and incubation time. (qualitydigest.com)
  • Today, lipid research has started to map the lipidome of human cells and describe lipid biological pathways. (sanbio.nl)
  • The objective of this study was to examine the influence of component of solid lipid nanoparticles on the viability of breast cancer cells using the MTT assay. (scialert.net)
  • The test substance was dosed by volumetric addition using a stock solution of the test substance prepared in NANOpure® water at a nominal concentration of 2000 mg/L. This method was deemed appropriate based on the solubility of the test substance in water. (europa.eu)
  • Nominal test concentrations must be prepared and reported in consideration of any such salinity adjustments. (canada.ca)
  • Clearly, we need standardization in all hormone assays. (medscape.com)
  • Three studies of DCM-exposed workers (ranging from 20 to 96 workers) have reported a positive correlation with urinary DCM (although small amounts) and time- weighted average DCM in the breathing-zone air of the workers (reviewed by Imbriani and Ghittori, 2005). (who.int)
  • Practically, it consists in blood collection from a patient receiving a drug, serum/plasma assay of the drug concentration and comparison with a therapeutic range (Gross, 1998). (vin.com)
  • Biological weighting functions for the response of microbial community to the exposure to UV radiation and its subsequent recovery are determined. (europa.eu)
  • However, in occupational set-up, humans are exposed to ATZ at a thousand-fold higher concentration than seen in residential exposure. (frontiersin.org)
  • Coauthor Hubert Vesper, PhD, who is cochair of the Partnership for the Accurate Testing of Hormones (PATH), an Endocrine Society initiative, commented, "Without harmonized reference ranges and standardized assays, tests can lead to misdiagnoses, and unfortunately this happens every day around the world. (medscape.com)
  • In young females, an estradiol level below the premenopausal reference range indicates hypogonadism . (medscape.com)
  • These methods cannot accurately reflect the changes in NO concentration in vivo and in real time. (intechopen.com)
  • A dose-response relationship was observed between ambient levels of DCM (8-hour TWA) and carboxyhemoglobin concentrations among cellulose triacetate production workers who were non-smokers (Amsel et al. (who.int)
  • There are good arguments it has become apparent that in addition to hazard that the standard against which new methods are identification, the dose-response data available being judged is much higher than those in place from the LLNA also permit further characteriza- when existing assays were adopted. (cdc.gov)
  • Validation of primer design is particularly important when adopting primers from a previous publication or using a commercially supplied assay. (sigmaaldrich.com)
  • Mouse splenocytes (500,000 cells/well) were activated with LPS (1 µg/mL) for 48 hours in mouse IL-6 ELISPOT assay. (thermofisher.com)
  • This results in differing cytotoxic responses that are influenced by the nature of target cells, assay types and concentrations of ATZ. (frontiersin.org)
  • As a follow-up of a previous investigation in which semisynthetic damsin derivatives were shown to possess up to 10 times higher cytoxicity in JIMT-1 breast cancer cells compared to normal breast epithelial MCF-10A cells, a range of new derivatives were prepared and assayed toward the same cells. (lu.se)
  • During a study involving the concentration of heavy metals, pesticides, and semi-volatile organic compounds in stream sediments from the Schuylkill River within the Valley Forge National Historical Park, the USGS used the ERL and ERM to determine locations that could potentially pose a threat to living organisms. (wikipedia.org)
  • In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering. (bellbrooklabs.com)
  • The chemical and biological study of plants is an alternative strategy in the search for new therapeutic agents for the identification of bioactive compounds. (ufrr.br)
  • The IC50 values in the two cell lines were found to depend on the overall structure of the assayed compounds, although less in this subset of compounds compared to a previous investigation. (lu.se)
  • If it is outside the range considered acceptable for the test (i.e., 28 to 32 g/kg), the salinity of the sample or each test solution should be adjusted to within this range using natural or artificial hypersaline brine, commercially-available dry ocean salts, reagent-grade salts (see Sections 2.3.4 and 4.3.2 ), or deionized water. (canada.ca)
  • Lipidomic techniques are highly utilised for mechanistic assays, risk prediction, and monitoring treatment efficacy. (sanbio.nl)
  • For the prediction of skin sensitization potential, the local lymph node assay (LLNA) is a fully validated alternative to guinea-pig tests. (cdc.gov)
  • The assays ranked in the same order in a validation cohort of 51 cognitively normal, 51 MCI, and 20 AD dementia samples from the Alzheimer's Disease Neuroimaging Initiative. (alzforum.org)
  • Steroid hormone analysis in blow (respiratory vapor) may provide a minimally-invasive way to assess the reproductive status of cetaceans in aquaria or the wild, but biological validation of the method is needed to allow for the interpretation of hormone measurements in blow samples. (vin.com)
  • A total of 167 samples collected from 12 females of various reproductive stages were assayed for progesterone. (vin.com)
  • No studies so far practically explored the sequence and mismatch detecting power of the fluorophore-labeled-oligo-nanoparticle conjugates in heterogeneous biological samples. (hindawi.com)
  • In this assay the Albumin present in samples reacts with the anti-Albumin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. (eaglebio.com)
  • Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles. (eaglebio.com)
  • The main purpose of TDM is to assist the practitioner in drug regimen adjustment to reach an optimal drug concentration ensuring an adequate therapeutic response without drug-induced adverse effects. (vin.com)
  • BACKGROUND: RBC folate concentrations are monitored at the population level, with a recommended threshold for optimal neural tube defect (NTD) prevention. (cdc.gov)
  • The pH and total suspended solids concentration of the activated sludge were determined on the day of use in the study. (europa.eu)
  • This work aimed to make the phytochemical study and biological assays of the species Davilla kunthii A. St.-Hil, considering that to date regarding chemical and biological records of this species were not found. (ufrr.br)
  • Defining the paradigms and predictable end point(s) enables selection of the assay formats and technologies to be used for the study. (genengnews.com)
  • We describe a collaborative study to investigate the comparability of serologic assays for MERS-CoV and assess any benefit associated with the introduction of a standard reference reagent for MERS-CoV serology. (cdc.gov)
  • Participants at the WHO intercountry meeting on MERS-CoV in Cairo, Egypt, June 20-22, 2013, recognized this issue as a public health priority and called for a study to compare currently available serologic assays ( 9 ). (cdc.gov)
  • Total bile acid (TBA) concentration is a commonly measured parameter to assess the severity of ICP. (randox.com)
  • To assess the performance of the RT-iPCR assay, non-infested soils representing a range of soil textures were collected from Alberta fields. (uleth.ca)
  • Two immunoprecipitation-mass-spectrometry assays came out on top, accurately pegging people who had plaques 83 to 87 percent of the time. (alzforum.org)
  • The data is arranged by ordering the concentrations from lowest to highest. (wikipedia.org)
  • The 10th percentile of the ranked data is identified as the ERL, and is considered indicative of concentrations below which adverse effects (relatively) rarely occur. (wikipedia.org)
  • The 50th percentile of the ranked data is identified as the ERM, and is indicative of concentrations above which adverse effects (relatively) frequently occur. (wikipedia.org)
  • In one run of the assay M molecules are tested, generating 2 M N data points. (physicsforums.com)
  • Can the 'standard deviation' or the assay itself be calculated from these data, as if they were direct, repeated measurements? (physicsforums.com)
  • Validation provides the data required to justify the continued use of the assay in further research projects 1 . (sigmaaldrich.com)
  • Collectively, these factors are known to influence data quality, biological relevance, and therapeutic predictability, which will ultimately impact the success of entire preclinical drug development efforts. (genengnews.com)
  • The data from this experiment was then used to narrow the concentration range for each compound, which was then tested in smaller increments. (umn.edu)
  • Actually, the phenolic hydroxyl group is utilized in medication adjustment and diversification frequently.5C17 We hypothesize that by converting a phenolic molecule with known biological actions to the matching arylfluorosulfate will serve as an instant and cost-effective way to recognize brand-new hits with improved properties. (baxkyardgardener.com)
  • however, current spectrophotometric methods only measure total nucleic acid concentrations. (qiagen.com)
  • This extension is the measurement of the scientific and regulatory community charged with relative skin sensitizing potency of a substance, the acceptance of new methods has to make prag- usually expressed as the estimated concentration matic decisions. (cdc.gov)
  • The contents of the first control vessel were transferred to a BOD bottle and dissolved oxygen concentrations were measured every 10 seconds over a 10 minute period, or until the DO measurement dropped below 1.0 mg O2/L using a YSI Model 50B-115 Dissolved Oxygen Meter. (europa.eu)
  • An extraction protocol was developed by spiking the soils with A. euteiches oospores and the RT-iPCR assay was used to quantify oospores in the soil extracts. (uleth.ca)