• This assay has been extensively used for different studies such as for preliminary toxicity screening of plant extracts, detection of fungal toxins, plant extract toxicity, heavy metals, cyanobacteria toxins, pesticides, and cytotoxicity testing of dental materials [ 9 ]. (biomedcentral.com)
  • Prediction of Chemical Carcinogenicity in Rodents from in Vitro Genetic Toxicity Assays R AYMOND W. TENNANT, BARRY H. MARGOLIN, MICHAEL D. SHELBY, E RROL Z … As an in vivo toxicity endpoint, the acute lung inflammogenicity in a rat instillation model was compared with the in vitro toxicity endpoints comprising cytotoxicity, pro-inflammatory cytokine expression, or haemolytic potential. (holocausto.online)
  • We conduct cytotoxicity estimation using a panel of cell proliferation and viability tests, which can be sorted into four types by different endpoints: Functional assays: assessment of ATP, ADP, ATP/ADP ratios. (holocausto.online)
  • Kroll A(1), Pillukat MH, Hahn D, Schnekenburger J. The in vitro assays covered baseline toxicity in bacteria, oxidative stress, cytotoxicity in mammalian cells and yeast, estrogenicity, and anti-androgenicity. (holocausto.online)
  • In vitro, chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc. (holocausto.online)
  • The negative results for cytotoxic and antimicrobial assays indicated possible low cytotoxicity to the extract. (researchgate.net)
  • Biological assays to test the cytotoxicity of the compound 1b combined with electroporation were performed to determine its potential for future medical applications in cancer treatment. (hindawi.com)
  • It has been proven that combining electroporation with chemotherapy potentiates the cytotoxicity of drugs when the drugs' efficacy is limited by its uptake in the cell [ 9 - 13 ]. (hindawi.com)
  • The aim of this study was to prepare and characterize new ruthenium NAMI-type compounds, to test their in vitro cytotoxicity and study the influence of electroporation on the cytotoxic activity of the synthesized compounds. (hindawi.com)
  • The search for targeted drugs for cancer treatment includes consideration of natural products, which may provide new opportunities for antitumor cytotoxicity as single agents or in combination therapy. (cancer.gov)
  • The ethanolic extracts of the plant were examined for the presence of bioactive components and their total flavonoid content, with focusing on quercetin detection using thin layer bioautography (TLB) and brine shrimp lethality assay (BSLA) for cytotoxicity. (who.int)
  • The same extracts were assessed for their antioxidative potentials with the use of DPPH free radical scavenging assay followed by determination of HRBC membrane stabilization method, Brine Shrimp Lethality Assay (BSLA) and GC-MS analysis. (biomedcentral.com)
  • During Brine Shrimp Lethality Assay, all extracts of BG were found to be bioactive and the degree of lethality was found to be concentration dependent. (biomedcentral.com)
  • The Brine Shrimp Lethality Assay found extracts to be bioactive suggesting extracts as a promising candidate for plant-derived anti-tumor compounds. (biomedcentral.com)
  • Studies have found very good relationship between this simple, inexpensive, and bench-top assay and the antitumor potential of the cytotoxic compounds [ 10 ]. (biomedcentral.com)
  • Despite the identification of tumor-specific somatic mutations, most cancer therapy still utilizes nonspecific, cytotoxic drugs. (broadinstitute.org)
  • As the American Chemical Society 's Mark Lesney glibly wrote back in 2002: "A large enough library with the right high-throughput screening assay promised the ultimate brute-force method for obtaining bioactive compounds without biologists and organic chemists getting in the way. (drugdiscoverynews.com)
  • Microorganisms have been considered to be a rich source of unique bioactive compounds. (microbialtec.com)
  • Nearly three-quarters of microbial-produced bioactive compounds are from actinomycete bacteria. (microbialtec.com)
  • Compounds of interest include not only classical "drug-like" organic small molecules, but also peptides, proteins, and other bioactive chemical classes. (cancer.gov)
  • Various natural compounds with promising in vitro and in vivo anti-inflammatory activities have been reported in literature which can be used as novel therapeutic approach for treatment of inflammatory conditions [ 7 ]. (biomedcentral.com)
  • In vitro toxicity assays are used to determine the potential of a new pharmaceutical, agrochemical, food additive, or other chemical product to be hazardous to humans.In vitro studies, which are performed on cultured bacterial or mammalian cells, can be used as an initial screen to avoid the unnecessary use of animals in determining which candidates should move forward for further safety testing. (holocausto.online)
  • The application of High Content Screening for in vitro toxicity testing is a relatively new approach in the preclinical research phase of drug development. (holocausto.online)
  • In regulatory testing situation these in vitro toxicity assays must be reliable, because from these tests it is only possible to generalize the data to … First Published 1995. (holocausto.online)
  • While toxicity test has been traditionally completed in the preclinical phase, in vitro toxicity studies in the early drug discovery stages could significantly reduce failures at a later stage and prevent economic loss. (holocausto.online)
  • Considering the ethical issues and the cost of in-vivo animal tests, the pharmaceutical industry now relies more on in-vitro methods for toxicity testing in the drug development phase. (holocausto.online)
  • Traditionally, the in vitro toxicity assays that have been used to identify molecules that carry drug-related DILI risk factors include two-dimensional (2D) cell systems and other standard preclinical model… The principal application of this assay is to rapidly screen test compounds to identify those that induce acute liver cell toxicity. (holocausto.online)
  • H … In vitro assays covered baseline toxicity, oxidative stress responses, and estrogenic and antiandrogenic activity. (holocausto.online)
  • The positive control induced a high degree of toxicity in all in vitro tests performed, indicating that the toxicity observed in the direct contact assay was due to in situ extraction of toxic components. (holocausto.online)
  • In vitro drug metabolism studies, are a screening mechanism to characterize drug metabolites, elucidate their pathways, to help with further in vivo testing. (td2inc.com)
  • A recent in vitro screening of the Phoneutria nigriventer spider venom (PnV) antitumor effects by our group has shown that the venom significantly affected glioblastoma cell lines. (nature.com)
  • Sphingolipid-targeting drugs have been tested alone or in combination with chemotherapy, exhibiting antitumor activity alone and in synergism with chemotherapy in vitro and in vivo . (frontiersin.org)
  • When I was at GSK and also when I was with Shire , one of the things I really had antibodies to was this whole notion of industrializing drug discovery-at the end of the day, that's really what combinatorial chemistry and HTS is or was-because I really believe that drug discovery of new medicines is truly a creative process," he says. (drugdiscoverynews.com)
  • The former issue was often neglected prior to initiating drug discovery programmes while the latter was considered impossible because it was purported that antibodies were unsuitable to derive compounds. (cancer.gov)
  • First that RAS is indeed druggable (as many labs have now shown, in particular KRASG12C binding compounds [13-15]) and that antibodies could indeed be used to guide the isolation of chemical matter with our Abd technology. (cancer.gov)
  • The GC-MS analysis of the methanolic extract of BG revealed 23 compounds which are reported to possess different biological activities. (biomedcentral.com)
  • Screening tools for the evaluation of a drug's ability to cross various biological membrane systems in a microplate format. (td2inc.com)
  • To confirm this popular use, ethanol extract from leaves of G. globosa L. was prepared by maceration and analyzed by some phytochemical and biological assays, including cardiovascular activity. (researchgate.net)
  • One such biological target was RAS, where decades of attempts had failed (until very recently) to yield anti-cancer drugs directly interfering with RAS function. (cancer.gov)
  • During his six year stay at GNF, Dr. Gray became the director of biological chemistry where he supervised a group of over fifty researchers integrating chemical, biological and pharmacological approaches towards the development of new experimental drugs. (stanford.edu)
  • There, he has established a discovery chemistry group that focuses on developing first-in-class inhibitors for newly emerging biological targets, including resistant alleles of existing targets, as well as inhibitors of well-validated targets, such as Her3 and RAS, that have previously been considered recalcitrant to small molecule drug development. (stanford.edu)
  • In a continuation of our work probing the anticancer activity of small sulindac libraries, we have prepared and screened a small diversity library of α-methyl substituted sulindac amides in the profen class. (nih.gov)
  • Microtubule binding drugs are of special interest as they have important roles in the modulation of cellular functions and many of them act as anticancer agents. (rgcb.res.in)
  • 50 years of preclinical anticancer drug screening:empirical to target-driven approaches. (jscimedcentral.com)
  • Anticancer drugs: Keeping one step ahead. (jscimedcentral.com)
  • The contribution of synthetic organic chemistry to anticancer drug development. (jscimedcentral.com)
  • We studied the chemosensitivity of these two MCSP tumor xenografts to anticancer drugs in vivo. (ox.ac.uk)
  • These results are consistent with the chemosensitivity of human MCSP to lipophilic anticancer compounds. (ox.ac.uk)
  • The development of platinum-drug resistance in cancer patients, the general toxicity and severe side effects of platinum drugs however required a different approach in the research of anticancer metal complexes [ 2 ]. (hindawi.com)
  • Protein binding assays assesses the degree to which drugs attaches to proteins within the plasma/tissue. (td2inc.com)
  • This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)
  • Of the 39 new molecular entities approved by the U.S. Food and Drug Administration (FDA) in 2012, only about a third of them would be classified as biologics or polypeptides. (drugdiscoverynews.com)
  • These include EpiScreen™, a T-cell epitope mapping technology that screens peptides for their immunostimulatory properties, and EpiScreen DC:T cell assay, which screens biologics for their immunogenicity. (genengnews.com)
  • Streptomycetes are the most widely identified group, producing a wide range of biologically active compounds. (microbialtec.com)
  • The bioassay-guided separation of the extract provided ten active compounds, including three new N-methyladenine-containing diterpenoids, agelasines W-Y ( 1 - 3 ), a new bromopyrrole alkaloid, N(1)-methylisoageliferin ( 4 ), and six known ageliferin derivatives ( 5 - 10 ). (cancer.gov)
  • Protein binding analysis by equilibrium dialysis of plasma across mutiple different speacies (mouse, rat, dog, monkely, human) following exposure to multiple compounds. (td2inc.com)
  • Our work on intracellular antibody fragments as tools for analysis of protein complexes argued that these macromolecules could also be employed for target validation in diseases and then as guides for target-specific surrogate compounds as inhibitors. (cancer.gov)
  • We wondered if these difficulties could be overcome by focussing on the antibody combining site (i.e. the part of antibody interacting with antigen) to guide the derivation of chemical compounds that would be surrogates of the antibody, because the combining site is the part relevant to the protein-protein interaction between antibody and antigen. (cancer.gov)
  • The corollary is that the specific interaction interface between the antibody fragment with target protein would be mimicked by the chemical compound surrogates. (cancer.gov)
  • Specific protein-binding measures are often used as assays in diagnostic assessments. (lookformedical.com)
  • Typically, low molecular weight drugs are not recognized by the immune system, whereas protein molecules of much higher molecular weight invariably are. (genengnews.com)
  • Organic chemistry in drug discovery. (jscimedcentral.com)
  • The susceptibility of but a few known transplacental car and reach the fetus, a carcinogenic the fetus relative to that of adult rats cinogens are organic compounds chemical may have no transplacen is measured as the incidence and that act principal y or entirely by a ge tal carcinogenic activity or may only multiplicity of tumours that develop notoxic mode of action. (who.int)
  • • Amura Holdings Ltd. , of Cambridge, UK, said preclinical study results showed that oral administration of the firm's cathepsin K inhibitor compounds resulted in sustained and potent reduction of serum bone turnover biomarkers, which clinically correlates with a significant reduction in fracture rates. (bioworld.com)
  • The objective of this review was to analyze the results from preclinical and clinical trials of these drugs for the treatment of cancer. (frontiersin.org)
  • Aslthough all these drugs have produced good results in preclinical studies of multiple cancers, the outcomes of clinical trials have not been similar. (frontiersin.org)
  • Here we describe a gene expression-based approach to identify compounds that induce a signature of EWS/FLI attenuation. (broadinstitute.org)
  • The Drug Repurposing Hub is one of the most comprehensive and up-to-date biologically annotated collections of FDA-approved compounds in the world. (broadinstitute.org)
  • Drug repositioning and pharmacophore identification in the discovery of hookworm MIF inhibitors. (jscimedcentral.com)
  • ChemDiv's Inhibitors of beta-Catenin Signaling Library contains 8,000 compounds. (chemdiv.com)
  • Proliferation assays However, in rats just minimal alterations were noted. (holocausto.online)
  • Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancerindex.org)
  • We hypothesize that screening small-molecule libraries highly enriched for FDA-approved drugs will provide a more rapid path to clinical application. (broadinstitute.org)
  • We demonstrate that a gene expression-based approach to small-molecule library screening can identify, for rapid clinical testing, candidate drugs that modulate previously intractable targets. (broadinstitute.org)
  • Two ruthenium compounds, NAMI-A and KP1019 [ 4 - 6 ], are currently among the most successful candidates to enter the clinical practice. (hindawi.com)
  • assists in identifying natural products and synthetic compounds that may serve as bioprobes for chemical genetics, proteomics, target validation and potential lead compounds for clinical development. (cancer.gov)
  • Here, we report the draft genomes for 45 Enterobacterales clinical isolates, including historical and contemporary drug-resistant organisms, obtained in Pakistan between 1998 and 2016: 5 Serratia, 3 Salmonella, 3 Enterobacter, and 34 Klebsiella. (cdc.gov)
  • The remainder were smaller chemical compounds ranging from classical small molecules to macrocyclics. (drugdiscoverynews.com)
  • With an eye to filling those drying pipelines with an endless supply of potentially therapeutic compounds, companies heavily invested in ways to randomly generate tens of thousands of "drug-like" compounds from the chemical building blocks of known drugs (see sidebar, "Leaving Lipinski? (drugdiscoverynews.com)
  • It is also worth noting that 52 percent (total) of these compounds are either a natural product, a mimic or a chemical modification of an existing natural product pharmacophore. (drugdiscoverynews.com)
  • This seemed much larger than would be expected for the range of space occupied by drug-like chemical compounds, estimated at 300 to 1000 Å2 [4, 5]. (cancer.gov)
  • Thus, our Abd technology identified a hit chemical series interacting with a pocket [10, 11] near the effector binding region in RAS (activated pan-RAS binding compounds) [9]. (cancer.gov)
  • The chemical compound series that was developed in this work is a pan-RAS series binding to all the RAS isoforms [12] because the binding pocket is found in KRAS, HRAS and NRAS. (cancer.gov)
  • Amongst all the marine invertebrates, sponges represent the most described group with potential application in medicinal chemistry, representing a valid starting point for new drug leads due to their chemical defense mechanisms. (microbialtec.com)
  • Nathanael Gray is the Krishnan-Shah Family Professor of Chemical and Systems Biology at Stanford, Co-Director of Cancer Drug Discovery Co-Leader of the Cancer Therapeutics Research Program, Member of Chem-H, and Program Leader for Small Molecule Drug Discovery for the Innovative Medicines Accelerator (IMA). (stanford.edu)
  • We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)
  • NSAIDS are widely used for their anti-inflammatory, analgesic and antipyretic activity and are among the most widely used drugs worldwide [ 6 ]. (biomedcentral.com)
  • Several compounds of this series displayed promising activity compared with a lead sulindac analog. (nih.gov)
  • An MTT assay was used to determine cell metabolic activity and a TUNEL assay for detecting DNA fragmentation. (holocausto.online)
  • Discovery pipelines have been horribly inefficient, and if you look at the major pharmas, what they're doing typically is dismantling that industrial framework and moving much more toward either internal-focused discovery with very small discovery units-and that's certainly how GSK's done it-or partnering with academic units or highly creative companies like Ambrx to bring forward those creative molecules as rational drug design from different disciplines converges," he adds. (drugdiscoverynews.com)
  • Molecules from animal venoms are promising candidates for the development of new drugs. (nature.com)
  • Studies in progress are selecting the venom molecules with antitumor and immunomodulatory effects and trying to better understand their mechanisms. (nature.com)
  • The identification, optimization and synthesis of antineoplastic drugs from PnV molecules may lead to a new multitarget chemotherapy. (nature.com)
  • On any given day, the media streams are filled with companies announcing their latest biologic achievements in medicine, and given the high profile of these therapeutics in disease areas such as oncology and autoimmunity, it is hardly surprising if some people have forgotten the role of medicinal chemistry in the drug discovery process. (drugdiscoverynews.com)
  • Part of the reason for medicinal chemistry's disappearing act may be due to attempts by the pharmaceutical industry itself to move medicinal chemists to the back burner by automating molecular discovery and synthesis through methods such as combinatorial chemistry and high-throughput screening (HTS). (drugdiscoverynews.com)
  • Mechanism-based target identification and drug discovery in cancer research. (jscimedcentral.com)
  • The role of the medicinal chemist in drug discovery--then and now. (jscimedcentral.com)
  • Terry Rabbitts is Professor of Molecular Immunology in the Centre for Cancer Drug Discovery, Institute of Cancer Research, Sutton, London, UK. (cancer.gov)
  • The latter discovery was important for us because it suggests that the antibody combining sites could generally be accessible for implementing the Abd technology approach to drug discovery. (cancer.gov)
  • Researchers anywhere can explore more than 6,000 drugs in the hub and search for possible new uses for them to jump-start new drug discovery. (broadinstitute.org)
  • A typical workflow for conventional bioprospecting and drug discovery. (microbialtec.com)
  • Further more, one of the most recent approaches in drug discovery from microorganisms is cocultivation. (microbialtec.com)
  • If you are interested in microbial drug discovery services, please contact us for more details. (microbialtec.com)
  • and discovery that sphingosine-1-phosphate receptor-1 (S1P1) is the pharmacologically relevant target of the immunosuppressant drug Fingomilod (FTY720) followed by the development of Siponimod (BAF312), which is currently used for the treatment of multiple sclerosis. (stanford.edu)
  • The Molecular Targets Program (MTP) provides the focus and infrastructure that enables CCR investigators to pursue molecularly targeted drug discovery research by promoting an interdisciplinary, collaborative, team-oriented approach to identifying and validating potential cancer-pertinent targets. (cancer.gov)
  • Therefore, Src has been considered a target molecule for drug development. (oncotarget.com)
  • These findings will assist the development of anti-tumour drugs to treat lung cancer. (oncotarget.com)
  • Billingsley ML. Druggable targets and targeted drugs: enhancing the development of new therapeutics. (jscimedcentral.com)
  • Hepatotoxicity (toxic effects on the liver) and cardiotoxicity (toxic effects on the heart) are the most severe problems encountered in drug development and the major reason for drug withdrawals. (holocausto.online)
  • Using X-ray crystallography soaking of KRASQ61H crystals by compounds, we implemented a structure-based compound development programme [9, 12]. (cancer.gov)
  • In 2021, Dr. Gray joined Stanford University where he has joined the Stanford Cancer Institute, Chem-H and the Innovative Medicines Accelerator (IMA) to spur the development of prototype drugs. (stanford.edu)
  • Characterisation of ER stress in PD models may be important for the current and future drug development of PD. (helsinki.fi)
  • If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
  • Additional screens for MMTV provirus entry sites in tumors revealed several other upregulated genes that are associated with other gene development pathways such as INT2, INT3, and INT4 [4,5,6]. (chemdiv.com)
  • Library has been assembled using in house structural biology insight, molecular stimulation-modeling, virtual screening of ChemDiv's novel chemistries and medicinal chemistry filtering/ranking of the resulting hits. (chemdiv.com)
  • The effectiveness or lack of a major therapeutic effect of sphingolipid modulation by some drugs as a cancer therapy and other aspects related to their mechanism of action are discussed in this review. (frontiersin.org)
  • This study aimed to identify the compounds that target Src to suppress lung cancer tumourigenesis and metastasis and investigate their underlying molecular mechanisms. (oncotarget.com)
  • Using a molecular docking approach and the National Cancer Institute (NCI) compound dataset, eight candidate compounds were selected, and we evaluated their efficacy. (oncotarget.com)
  • Guidebook on molecular modeling in drug design: Academic Press. (jscimedcentral.com)
  • Ooms F. Molecular modeling and computer aided drug design. (jscimedcentral.com)
  • assays indicated possible low cy to to xicity to the extract. (researchgate.net)
  • It includes also the names of 158 compounds isolated from higher plants, marine sponges and algae, fungi and snake venom. (scielo.br)
  • Previously, this target has been intractable to modulation with traditional small-molecule library screening approaches. (broadinstitute.org)
  • Dr. Gray has also developed structure-based, generalized approaches for designing drugs to overcome one of the most common mechanisms of resistance observed against most kinase inhibitor drugs, mutation of the so-called 'gatekeeper' residue, which has been observed in resistance to drugs targeting BCR-ABL, c-KIT and PDGFR. (stanford.edu)
  • While all of the isolated compounds showed Cbl-b inhibitory activities, ageliferins ( 4 - 10 ) were the most potent metabolites, with IC50 values that ranged from 18 to 35 μM. (cancer.gov)
  • Further applications are possible in various areas of pharmacology, developments catalyzed by the use of simple enzyme assays for screening before testing in intact animals. (microbialtec.com)
  • Non-steroidal anti-inflammatory drugs (NSAIDs) have a variety of potential indications that include management of pain and inflammation as well as chemoprevention and/or treatment of cancer. (nih.gov)
  • Patients can usually be treated with nonsteroidal anti-inflammatory drugs (NSAIDs) alone. (msdmanuals.com)
  • Since in classic chemotherapies it is observed a decrease in immune response, methotrexate (MTX) was used only to compare the PnV effects on innate immune cells with a highly immunosuppressive antineoplastic drug. (nature.com)
  • Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • Side effects of concern are infectious diseases, which prompt the need for screening for latent mycobacterial infection before commencing treatment ( 7 ). (cdc.gov)
  • However, with an adeq battery of necf expsre blmarkers, prospective studies of en- vironmental effects on pregnancy outcomes might be possible. (cdc.gov)
  • This low survival may result from the metastasis of cancer cells and arising resistance to drugs. (oncotarget.com)
  • Li X, Taratula O, Taratula O, Schumann C, Minko T. LHRH-Targeted Drug Delivery Systems for Cancer Therapy. (jscimedcentral.com)
  • We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
  • Drugs of natural origin have been classified as (i) original natural products, (ii) products derived or chemically synthesized from natural products or (iii) synthetic products based on natural product structures. (microbialtec.com)
  • Based on these factors, it seemed possible that compounds that bind to antigens could be selected using Dab binding sites and that these compounds would be surrogates of the Dab. (cancer.gov)
  • Notably, the tuberculin skin test result was negative at screening before she began the TNF-αA treatment, but was now positive (20 mm), suggesting mycobacterial infection. (cdc.gov)
  • Methods The literature related to PMS were collected from China Knowledge Network (CNKI), Wanfang database and Weipu database in the past 20 years, the herbal compound prescriptions for the treatment of PMS were screened and a relevant database were established and analyzed by SPSS. (bvsalud.org)
  • This has led to sudden increase in the number of herbal industries in the drug market. (pharmatutor.org)
  • Results The relevant literatures contains 184 Chinese medicine prescriptions/proprietary Chinese medicines, 122 flavors of traditional Chinese medicines, and the drug categories were mainly tonic drugs, antipyretic drugs, astringent drugs, and tranquilizers. (bvsalud.org)
  • We exploited the specific binding of the antibody fragment and were able to identify RAS-binding compounds in antibody-competition assays (illustrated in Fig.1). (cancer.gov)
  • Dr. Gray's research has had broad impact in the areas of kinase inhibitor design and in circumventing drug resistance. (stanford.edu)
  • A number of microbial compounds capable of suppressing the immune response have been discovered. (microbialtec.com)
  • In the 25 years from 1981 to 2006, they suggest, only one de-novo compound arising from combinatorial chemistry achieved regulatory approval, that being Bayer 's antitumor compound sorafenib. (drugdiscoverynews.com)
  • A small-molecule library enriched for FDA-approved drugs was screened with a high-throughput, ligation-mediated amplification assay with a fluorescent, bead-based detection. (broadinstitute.org)
  • Here, we first used high-throughput screening and had screened one compound candidate, ebastine (a H1-histamine receptor antagonist), for osteosarcoma therapy. (ijbs.com)
  • When RON MET TAM in Mesothelioma: All Druggable for One, and One Drug for All? (frontiersin.org)
  • Our researchers are now extracting bacterial DNA from soil and marine habitats, cloning large fragments into, for example, bacterial artificial chromosomes, expressing in a host bacterium and screening the library for new compounds. (microbialtec.com)
  • Mammalian genotoxicity assay reveals if the compound causes abnormalities in chromosome distribution (aneugenity) or chromosome breaks (clastogenity) during cell division. (holocausto.online)
  • A cell monolayer grown on a filtered-well is used to evaluate test compound movement between the insert (apical compartment -A) and the plate well (basal compartment -B ). Passive transport through the layer is determine by A-B flux, whereas active transporter will introduce a B-A distribution of compounds between the two compartments. (td2inc.com)
  • Treatment options are limited at best and drug resistance is common. (frontiersin.org)
  • Therefore, a rationale for treating hypertension would be to administer drugs or natural compounds which selectively inhibit ACE. (scielo.br)
  • Some aspects of recent research with natural products directed to produce anti-hypertensive drugs are discussed. (scielo.br)
  • These tools were initially used in RAS target validation and subsequently for isolating drug leads, guided by the intracellular antibody interaction with RAS. (cancer.gov)