• protein
  • Making use of a protein found in animals which has evolved to selectively bind metabolites, an assay was developed which better classified samples compared to current methods used in the field of metabolomics. (montana.edu)
  • Methods -A Western blot assay was conducted to detect serum antibodies against the cytotoxin using recombinant cytotoxin (VacA protein) as an antigen. (bmj.com)
  • In order to assess the serum antivacuolating cytotoxin antibody which is thought to indicate infection with cytotoxin positive H pylori strains, we have cloned the vacA gene, purified the recombinant cytotoxin protein expressed in Escherichia coli , and conducted a western blot assay to detect the antibody against the toxin in patients with and without various gastrointestinal diseases. (bmj.com)
  • So they started to develop a biomolecule toolkit for the storage and search of biological entities such as peptides, proteins, protein complexes or bioconjugates. (chemaxon.com)
  • As a discipline, proteomics has moved past rapid protein identification and has developed into a biological assay for quantitative analysis of complex protein samples by comparing protein changes in differently perturbed systems. (wikipedia.org)
  • Chemical biologists are poised to impact proteomics through the development of techniques, probes and assays with synthetic chemistry for the characterization of protein samples of high complexity. (wikipedia.org)
  • An intrinsic binding affinity of the Strep-tag towards Strep-Tactin results in a highly specific interaction, which enables the isolation and purification of sensitive proteins in a native state as wells as intact protein complexes, respectively. (wikipedia.org)
  • They consist of non-magnetic soluble protein complexes generated by multimerization of αCD3- and αCD28 Fab-Streps with a Strep-Tactin multimer. (wikipedia.org)
  • Isolate the DNA bound protein by EMSA: the DNA bound protein, migrated in acrylamide gel, is isolated by autoradiography as per an Electrophoretic mobility shift assay (EMSA) protocol. (wikipedia.org)
  • The protein bound DNA was detected by autoradiography, and the bands representing protein-DNA complexes were excised from the gel and the eluted DNA were amplified by PCR using primers complementary to the 20 bp nonrandom flanking sequences. (wikipedia.org)
  • Probably it functions as a mediator of solubility for hydrophobic sHsps and it stabilizes the protein and protein/substrate complexes. (wikipedia.org)
  • In vitro it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding by the Hsp70-complex. (wikipedia.org)
  • reagents
  • If the assay involves addition of exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity (and quality) of the target is the only limiting factor for the reaction/assay process, and the difference in the assay outcome is used to deduce the unknown quality or quantity of the target in question. (wikipedia.org)
  • repositories
  • PerkinElmer Datalytix™ software is specifically designed to import relevant data into TIBCO Spotfire from scientifically significant data sources such as compound registries, biological assay repositories, LIMS systems, and other corporate information systems. (cambridgesoft.com)
  • proteins
  • Since the relatively few microRNAs in the genome are thought to directly regulate a large portion of the proteome, miRNAome screening, coupled with the identification of the regulated proteins, might be a powerful new approach to gaining insight into complex biological processes. (frontiersin.org)
  • processes
  • Key to this service is the ability for TTP's team to understand the fundamental steps required to convert lab-based biological processes (for example, sample preparation, labelling and assay measurement) to a functional low-cost disposable and small instrument. (technologynetworks.com)
  • For example, one platform enables open-access biology and liquid handling for 30 µL to 2 mL volumes, while another is purpose-built for dealing with complex processes such as small liquid volumes in the range of 10 µL to 200 µL whilst maintaining a low disposable cost. (technologynetworks.com)
  • Such industrial scale assays as these are often done in well equipped laboratories and with automated organization of the procedure-from ordering an assay to pre-analytic sample processing (sample collection, necessary manipulations e.g. spinning for separation or other processes, aliquoting if necessary, storage, retrieval, pipetting/aspiration etc. (wikipedia.org)
  • Isothermal microcalorimetry (IMC) is a laboratory method for real-time monitoring and dynamic analysis of chemical, physical and biological processes. (wikipedia.org)
  • Therefore, in order to be sure what process or processes are producing the measured heat flow, great care must be taken in both experimental design and in the initial use of related chemical, physical and biologic assays. (wikipedia.org)
  • Mathematical biology aims at the mathematical representation, treatment and modeling of biological processes, using techniques and tools of applied mathematics. (wikipedia.org)
  • Development
  • The report covers biological products in development, biological products in clinical trials, and currently marketed and late-stage development biologic cancer products. (bccresearch.com)
  • The new service provides an accessible and rapid route to market, offering a seamless approach for companies looking to accelerate the development of their assay to a product for use in diagnostics or research. (technologynetworks.com)
  • We're confident that this service and related TTP platforms will prove attractive to assay and instrument providers in this emerging market, by providing an accelerated route to manufacture that is augmented by our expertise in product development, engineering and the underlying biology of assay design and development. (technologynetworks.com)
  • Those assays that are very highly commercially demanded have been well investigated in research and development sectors of professional industries, undergone generations of development and sophistication, and become copyrighted intellectual properties via highly competitive process patenting. (wikipedia.org)
  • Medicinal chemistry and pharmaceutical chemistry are disciplines at the intersection of chemistry, especially synthetic organic chemistry, and pharmacology and various other biological specialties, where they are involved with design, chemical synthesis and development for market of pharmaceutical agents, or bio-active molecules (drugs). (wikipedia.org)
  • It includes synthetic and computational aspects of the study of existing drugs and agents in development in relation to their bioactivities (biological activities and properties), i.e., understanding their structure-activity relationships (SAR). (wikipedia.org)
  • While a number of approaches toward the identification and development of hits exist, the most successful techniques are based on chemical and biological intuition developed in team environments through years of rigorous practice aimed solely at discovering new therapeutic agents. (wikipedia.org)
  • Theoretical biology focuses more on the development of theoretical principles for biology while mathematical biology focuses on the use of mathematical tools to study biological systems, even though the two terms are sometimes interchanged. (wikipedia.org)
  • cell-based
  • With the advent of whole genome libraries of miRNA mimics suitable for high throughput screening, it is possible to comprehensively evaluate the function of each member of the miRNAome in cell-based assays. (frontiersin.org)
  • synthesis
  • This allows for straightforward construction of complex polyaryl systems, an attractive method for library synthesis of drug targets containing functionalized pyridine moieties. (wikipedia.org)
  • In addition, hits also routinely originate from structural observations of small molecule "fragments" bound to therapeutic targets (enzymes, receptors, etc.), where the fragments serve as starting points to develop more chemically complex forms by synthesis. (wikipedia.org)
  • Methods
  • This includes technologies that provide benefits for a number of assay steps, including biological and cellular deposition, methods for the rapid lysing of high sample volumes, high speed thermocycling, optical and electronic detection systems, and unique low profile and non- pulsatile pumps for highly controlled liquid movement. (technologynetworks.com)
  • aims
  • The assay usually aims to measure an intensive property of the analyte and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of some effect in comparison to a standard, etc. (wikipedia.org)
  • biology
  • Users of the Desktop Biology service will have the opportunity to benefit from TTP's in-house platforms and technologies, that have been designed to translate complex biological assays into low-cost, disposable consumables. (technologynetworks.com)
  • Desktop Biology customers will also have access to TTP's novel IP that has been especially developed to address some of the key challenges in the biological arena. (technologynetworks.com)
  • The field is sometimes called mathematical biology or biomathematics to stress the mathematical side, or theoretical biology to stress the biological side. (wikipedia.org)
  • properties
  • To address this problem we have developed a series of robust, self-assembled gallium/lanthanide metallacrown complexes that show exceptional luminescence properties in the visible and near-infrared regions with many options for multiplex analysis. (umich.edu)
  • time
  • Terbium and Rhodamine as Labels in a Homogeneous Time-resolved Fluorometric Energy Transfer Assay of the .beta. (patentgenius.com)
  • In biological specimens containing a growth medium, appearance over time of a detectable and rising heat flow signal is a simple general indicator of the presence of some type of replicating cells. (wikipedia.org)
  • laboratory
  • Since the assay itself (the analytic step) gets much attention, steps that get less attention by the chain of users, i.e. the preanalytic and the post analytic steps, are often less stringently regulated and generally more prone to errors - e.g. preanalytic steps in medical laboratory assays may contribute to 32-75% of all lab errors. (wikipedia.org)
  • predict
  • Thus, it was hypothesized that the chelating strength of these substances could be used to predict their biological activity on bacterial cells. (diva-portal.org)
  • field
  • Assays have become a routine part of modern medical, environmental, pharmaceutical, forensic and many other businesses at various scales from industrial to curbside or field level. (wikipedia.org)
  • test
  • test the quality of", from Anglo-French assaier, from assai (n.), from Old French essai "trial", and the noun assay thus means "trial, test of quality, test of character", mid-14th century, from Anglo-French assai and the meaning "analysis" is from the late 14th century. (wikipedia.org)
  • steps
  • The information communication (e.g. request to perform an assay and further information processing) or specimen handling (e.g. collection, transport and processing) that are done until the beginning of an assay are the preanalytic steps. (wikipedia.org)
  • Similarly, after the assay, the result may be documented, verified and transmitted/communicated in steps that are called post-analytic steps. (wikipedia.org)
  • i.e. not only analytic variations and errors intrinsic to the assay itself but also variations and errors involved in preanalytic and post analytic steps. (wikipedia.org)
  • possible
  • By combining the power of random-sequence selection with pooled sequencing, the SAAB imprint assay makes possible simultaneous screening of a large number of binding site mutants. (wikipedia.org)
  • often
  • Antibodies in human sera against the vacuolating cytotoxin have often been assessed by using the neutralising assay. (bmj.com)
  • scale
  • The methodology of the neutralising assay, however, is too complicated for large scale analysis. (bmj.com)
  • technology
  • Using our metallacrown antennae technology, the highest quantum efficiencies ever reported for a solid-state Yb 3+ or Er 3+ complex were achieved and excellent photophysical data were reported for several other lanthanides. (umich.edu)
  • One approach is to use high throughput biological screening technology to probe the nodes and networks, providing experimental validation of the computationally determined networks. (frontiersin.org)
  • further
  • The results further show that the Ga-ME0329 complex reduced ExoS expression and secretion to a higher extent than Ga-citrate, Ga-ME0163 or the non-coordinated hydrazone. (diva-portal.org)
  • data
  • In principle, researchers will be able to utilize these data to formulate and answer complex biological questions-including important questions in cardiovascular medicine. (frontiersin.org)
  • As of November 2012, the Gene Expression Omnibus ( http://www.ncbi.nlm.nih.gov/geo/ ) lists 2720 datasets covering over 800,000 assays while ArrayExpress at European Bioinformatics Institute contains data from 33,868 datasets covering nearly a million assays ( http://www.ebi.ac.uk/arrayexpress/ ). (frontiersin.org)
  • As a scientist, you know what it's like to struggle with vast amounts of complex data from a multitude of sources. (cambridgesoft.com)
  • however
  • However, the effect against biofilm formation by P. aeruginosa , by the ME0329 complex, was similar to Ga-citrate and lower than what has been reported for Ga-ME0163. (diva-portal.org)
  • similar
  • Cephalopods such as the octopus have complex chromatophore organs controlled by muscles to achieve this, whereas vertebrates such as chameleons generate a similar effect by cell signalling. (wikipedia.org)