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OligonucleotidesOligodeoxyribonucleotidesNucleic Acid HybridizationBase SequenceNucleic Acid ConformationDNAOligonucleotides, AntisenseMolecular Sequence DataOligoribonucleotidesPhosphorothioate OligonucleotidesOligodeoxyribonucleotides, AntisenseOligonucleotide ProbesThionucleotidesNucleic Acid DenaturationOligoribonucleotides, AntisenseRNA, MessengerRibonuclease HOrganophosphorus CompoundsRNADNA, Single-StrandedPolymerase Chain ReactionNucleic AcidsCloning, MolecularAmino Acid SequenceDNA, AntisenseRibonuclease T1GuanineBinding SitesIndicators and ReagentsDNA-Binding ProteinsBase PairingIntercalating AgentsTranscription, GeneticDNA PrimersKineticsRibonucleasesBase CompositionCell LineTransfectionPsoralensDNA ProbesGenes, SyntheticPhosphoric AcidsEscherichia coliHydrogen CyanideBiogenesisEncyclopedias as TopicEvolution, ChemicalRNA, CatalyticOxidoreductases Acting on CH-NH2 Group Donors
SequencesProbesCDNAPolymeraseMicroarraysSingle-strandedAntisense oligonucleotidesFragmentsHybridisationStrandsHybridizeBindsSequenceSyntheticFluorophoreMoleculeSynthesisTemperatureTargetArraysMRNAClinicalBasesProbeMethodsTechnologyDesignResultsSpecificallyResearchServe
Sequences14
  • This process is repeated to build specific oligonucleotide sequences. (medscape.com)
  • Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. (sigmaaldrich.com)
  • RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
  • The probes contain complementary sequences that hybridize to the target sequence. (genomeweb.com)
  • Only when hybridisation is complete, protein-specific DNA reporter sequences are formed by enzymatic extension of the oligonucleotides, which are detected by amplification using qPCR. (unimedizin-mainz.de)
  • Molecular biologists describing DNA sequences or referring to one of the two strands of double- stranded DNA frequently use complementary pairs of terms, such as coding/ non- coding, sense/ nonsense or transcribing/ non- transcribing. (genomicglossaries.com)
  • Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
  • The microarray technology is based on the hybridization of labelled DNA or RNA molecules to complementary sequences that are immobilized on a solid substrate. (tutorialspoint.com)
  • The principle of DNA microarray technology is based on the hybridization of complementary sequences of nucleic acid molecules. (tutorialspoint.com)
  • The probes are complementary to specific sequences of DNA or RNA molecules that are to be analysed. (tutorialspoint.com)
  • The hybridization occurs between the complementary sequences of the probes and the target molecules. (tutorialspoint.com)
  • The DNA microarray contains thousands of known DNA sequences that are complementary to the DNA of the sample being tested. (tutorialspoint.com)
  • The DNA from the sample binds to the complementary sequences on the microarray, and the amount of bound DNA is measured to determine the expression level of each gene in the sample. (tutorialspoint.com)
  • The cDNA microarray uses complementary DNA as probes, while the oligonucleotide microarray uses short DNA sequences as probes. (tutorialspoint.com)
Probes8
  • In the study, the researchers used the DxSeq capture technology, which involves the use of linear oligonucleotide probes. (genomeweb.com)
  • The four oligonucleotide probes in the LCx assay recognize and hybridize to a specific target sequence within the C. trachomatis plasmid DNA. (cdc.gov)
  • The oligonuleotides are designed to be complementary to the target sequence so that in the presence of target, the probes will bind adjacent to one another. (cdc.gov)
  • TaqMan®) probes, incorporate a 5' reporter fluorophore and a 3' quencher on a short oligonucleotide complementary to the target sequence. (neb.com)
  • The probes can be cDNA fragments or oligonucleotides, depending on the type of microarray. (tutorialspoint.com)
  • Molecular beacons are hairpin shaped fluorescent oligonucleotide probes that can report the presence of a completely complimentary DNA target. (usda.gov)
  • Dual Labeled Fluorescent Probes are DNA oligonucleotides of 20-30 bp carrying a fluorophore (5'-end) and a quencher (3'-end). (jenabioscience.com)
  • DNA from a patient is compared to a reference genome using many oligonucleotide probes. (msdmanuals.com)
CDNA6
  • The introduction of complementary DNA (cDNA) microarray technology in 1995 has helped to facilitate the identification and classification of DNA sequence information and the assignment of functions to these new genes by allowing investigators to analyze expression of thousands of genes simultaneously in a single experiment. (medscape.com)
  • Messenger RNA (mRNA) from the sample of interest can serve as a template for producing complementary DNA (cDNA) in the presence of a reverse transcriptase enzyme. (medscape.com)
  • The 2 most common microarray technologies in use are the oligonucleotide microarrays and the robotically spotted complementary DNA (cDNA) microarrays. (medscape.com)
  • The reaction mixture is heated to 37 ˚C, which allows for the production of complementary cDNA copy from the RNA sample by reverse transcriptase. (sigmaaldrich.com)
  • There are two main types of DNA microarrays: cDNA microarrays and oligonucleotide microarrays. (tutorialspoint.com)
  • The two main types of DNA microarray are the cDNA microarray and the oligonucleotide microarray. (tutorialspoint.com)
Polymerase8
  • A standard PCR consists of target DNA, a set of synthetic oligonucleotide primers that flank the target DNA sequence, a thermostable DNA polymerase (usually Taq polymerase), and nucleotides. (sigmaaldrich.com)
  • 87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
  • Extension of the dual-labeled primer by the polymerase creates a copy of the complementary target sequence. (neb.com)
  • The complementary DNA is prepared from messenger RNA of retroviruses utilizing particular enzyme contrary RNA polymerase. (free-essays.us)
  • Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
  • [ 1 ] The resultant complementary DNA is amplified by polymerase chain reaction (PCR). (medscape.com)
  • Initially, DNA is taken from the clinical specimen, as well as certain sequence-specific oligonucleotide primers, thermostable DNA polymerase, nucleotides, and buffer. (medscape.com)
  • Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
Microarrays2
  • Oligonucleotide microarrays are manufactured by Affymetrix (Santa Clara, Calif) using photolithographic techniques. (medscape.com)
  • Oligonucleotide microarrays are prepared by synthesizing oligonucleotides directly on the microarray chip. (tutorialspoint.com)
Single-stranded1
  • Gillespie and Spiegelman (1) observed that single stranded DNA binds strongly to nitrocellulose membranes in a manner that minimises the two strands reassociating with each other, but allows the hybridisation to complementary RNA. (ddw-online.com)
Antisense oligonucleotides2
Fragments1
Hybridisation1
  • The method is based on the removal of wild type alleles by hybridisation to immobilised complementary oligonucleotides. (degruyter.com)
Strands3
  • This is a simple two-state, all-or-nothing model that does not consider other possibilities besides the complete Watson-Crick pairing between self-complementary strands, which is a reasonable assumption if the oligonucleotide is relatively short. (weebly.com)
  • Following PCR, the Harvard team hybridised the DNA strands (to form a double helix) with complementary strands coupled to beads. (chemistryworld.com)
  • G-rich DNA strands are naturally involved in duplex-DNA architecture through association with their complementary C-rich DNA strands by the canonical Watson-Crick pairing [ 1 - 3 ]. (hindawi.com)
Hybridize1
  • A PCR primer consists of two oligonucleotides that hybridize to complementary stands of the DNA template, and thus identify the region to be copied. (ojp.gov)
Binds2
  • A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
  • The tagged DNA binds to its complementary DNA segment and can be detected by measuring the amount and type of fluorescence. (msdmanuals.com)
Sequence3
  • Highly specific and effective gene silencing of any disease can be achieved by an accurate knowledge of the target mRNA sequence and rational design of its complementary antisense agents for the downregulation of its protein message. (genomicglossaries.com)
  • The labeled probe hybridizes sequence-specifically to its complementary section of the amplicon. (jenabioscience.com)
  • Moreover, speci®c hy- found in narrow minor groove regions in a variety dration patterns seem to play a role in nucleotide sequence recognition by proteins (Otwinowski of oligonucleotide duplexes (Prive et al. (lu.se)
Synthetic1
  • A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
Fluorophore1
  • When the molecular beacon is not annealed to a complementary target the hairpin structure places the fluorophore and the quencher in close proximity preventing fluorescence. (usda.gov)
Molecule3
  • RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
  • A huge diversity of organic molecules can be synthesized efficiently where each individual molecule carries a unique oligonucleotide tag serving as amplifiable barcode. (novartis.com)
  • The oligonucleotide tag provides single-molecule detection by allowing amplification and sequencing for hit identification from in-vitro affinity screening of encoded libraries of immense diversity. (novartis.com)
Synthesis1
Temperature1
  • Learn about methods for calculating oligonucleotide melting temperature (Tm). (sigmaaldrich.com)
Target3
  • 2) Designing, synthesizing, and screening of a oligonucleotide-tagged library to increase our understanding of efficient target modulation. (novartis.com)
  • Oligonucleotide which displays specific binding to a protein or other target, often selected by an iterative cycle of affinity- based enrichment. (genomicglossaries.com)
  • The method is based on a magnetic barcoding strategy: PCR-amplified target MTB genes are captured and magnetically labeled by a pair of complementary oligonucleotides conjugated to microspheres and magnetic nanoprobes. (cdc.gov)
Arrays1
  • We used oligonucleotide arrays to analyze miRNA expression in the murine hematopoietic system. (nih.gov)
MRNA1
Clinical2
  • CordenPharma brings into the collaboration the infrastructure, capacity and expertise in the cGMP manufacturing of Oligonucleotides to support customers from early clinical stage to commercial market supply ​," CordenPharma's international VP of global marketing and sales Michael Quirmbach told us. (outsourcing-pharma.com)
  • According to Leoillot, Oligonucleotides were a promising new product family about 15 years ago, but have faced several clinical challenges. (outsourcing-pharma.com)
Bases1
  • [ 7 ] They consist of a glass surface onto which oligonucleotides consisting of 25 bases are built a single nucleotide at a time. (medscape.com)
Probe1
Methods2
  • 1) Proof-of-concept for combining complementary lead discovery methods in synergy with novel encoded chemistry. (novartis.com)
  • Methods for UV filter analysis followed the principles of European standardized methods for pesticide residue analysis (EN 15289). (researchgate.net)
Technology2
Design1
Results1
  • 3) Leveraging the results from oligonucleotide-encoded screening by synthesizing corresponding bioactive small molecules that can modulate protein function. (novartis.com)
Specifically1
  • We employed an antisense oligonucleotide (ASO) strategy in an effort to specifically inhibit the expression of PTEN. (diabetesjournals.org)
Research1
  • Recently the research workers developed complementary DNA from chemical procedure. (free-essays.us)
Serve1
  • CordenPharma and GE Healthcare have teamed up to serve the fast growing Oligonucleotide market with complementary offerings. (outsourcing-pharma.com)