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BuffersCompartment SyndromesCell CompartmentationEndosomesHydrogen-Ion ConcentrationGolgi ApparatusEndocytosisTromethamineKineticsProtein TransportLysosomesModels, BiologicalBiological TransportCell MembraneBody Fluid CompartmentsEndoplasmic ReticulumSubcellular FractionsMolecular Sequence DataVacuolesCell LineMembrane ProteinsMicroscopy, FluorescenceMicroscopy, ElectronCells, CulturedHEPESFasciaOrganellesAmino Acid SequenceTime FactorsCytoplasmIntracellular MembranesCalciumBrefeldin Arab GTP-Binding ProteinsMicroscopy, ConfocalCell NucleusMicroscopy, ImmunoelectronCell FractionationProtein BindingCytosolTemperatureMutationRecombinant Fusion ProteinsGreen Fluorescent ProteinsVesicular Transport Proteinstrans-Golgi NetworkTransport VesiclesSignal TransductionFluorescent Antibody TechniqueMice, Inbred C57BLFluorescent DyesOsmolar ConcentrationImmunohistochemistryHeLa CellsCarrier ProteinsSolubilityChromatography, High Pressure LiquidCattleLysosome-Associated Membrane GlycoproteinsRabbitsRats, Sprague-DawleyMolecular WeightElectrophoresis, Polyacrylamide GelTransferrinCricetinaeReceptors, TransferrinLiverBase SequenceIntracellular FluidProteinsTransfectionMonensinBicarbonatesAmmonium ChlorideMembrane GlycoproteinsPhagosomesTissue DistributionProtein Structure, TertiaryCell DifferentiationDiffusionCytoplasmic VesiclesLuminescent ProteinsRecombinant ProteinsBiological Transport, ActivePhosphatesOxidation-ReductionAdenosine TriphosphateFlow CytometryEgtazic AcidBlotting, WesternMitochondriaEpithelial CellsCercopithecus aethiopsIndicators and ReagentsElectrophoresis, CapillaryProtein Processing, Post-TranslationalMice, KnockoutExocytosisExtracellular SpaceIntra-Abdominal Hypertension
Phosphate-bufferIntracellular compartmentsStorage compartmentsExtracellularReagentsRepresentsAntibodiesTissueSynthesisSystemCellsProductActivityMajorSizeRelationshipLowerModelBodyPartitionIntracellular Fluid CompartmentExtracellular fluid compartmentBody fluid comparSubcellularGaylord ArchivalLysisSequentiallyTissuesProteinsBatteryDistinctDiffusionIsolationTubesShelfConcentrationSamplesSolutionButtstockProbeSeparateSystemsShutterModelPressureStorageControlsPreviousAdditionHelpsVolumeHoursHeatChoiceCircuitWeightMakeFootDataBalance
Phosphate-buffer2
  • Split-thickness human cadaver skin was mounted onto non occluded Franz diffusion cells with the stratum corneum (SC) facing the donor compartment and the dermis contacting the receptor compartment, which was filled with phosphate-buffered saline, pH 7.4, preserved with 0.02% NaN3 (PBS) and magnetically stirred. (cdc.gov)
  • Honey and methadone samples were diluted in sterile phosphate-buffered saline and cultured with or without heat pretreatment (90°C, 10 min) under aerobic and anaerobic conditions at 37°C for 3-4 days by using Columbia blood agar and MYPGD (Mueller-Hinton broth, yeast extract, potassium phosphate, glucose, pyruvate) agar. (cdc.gov)
Intracellular compartments2
  • Biological membranes are impermeable to magnesium (and other ions), so transport proteins must facilitate the flow of magnesium, both into and out of cells and intracellular compartments. (wikipedia.org)
  • Total tacrolimus daily exposure (AUC 0-24 h ), in whole blood and intracellular compartments, was over-estimated when assessed by doubling the morning AUC 0-12 h data. (frontiersin.org)
Storage compartments1
  • These vertical and horizontal storage compartments are suitable for all panel sizes, from 100 mm 2 to 2x6 m. (fivesgroup.com)
Extracellular2
  • From recent work in humans and rats, the notion has emerged that Na+ could be stored somewhere in the body without commensurate water retention to buffer free extracellular Na+ and that previously unidentified extrarenal, tissue‐specific regulatory mechanisms are operative regulating the release and storage of Na+ from a kidney‐independent reservoir. (uib.no)
  • Taken together, observations of salt storage in the skin to buffer free extracellular Na+ and macrophage modulation of the extracellular matrix and lymphatics suggest that electrolyte homeostasis in the body cannot be achieved by renal excretion alone, but also relies on extrarenal regulatory mechanisms. (uib.no)
Reagents2
  • Imidazoles, Pyrroles, Pyrazoles, Pyrrolidines;Biochemistry;Reagents for Oligosaccharide Synthesis;Highly Purified Reagents;Other Categories;Zone Refined Products;Buffer;ACS Grade Buffers;ACS Grade;Biological Buffers;Buffers A to Z;Building Blocks;C3 to C8;Chemical Synthesis;Essential Chemicals;Heterocyclic Building Blocks;Imidazoles;Inorganic Salts;Research Essentials;Solutions and Reagents;Intermediate for Propafenone hydrochloride. (lookchem.com)
  • The dual reservoir adapter accommodates 2 divided reagent reservoirs with compartments for 4 different reagents. (integra-biosciences.com)
Represents1
  • The name compartment displays the name of the partition and the element that it represents, and the shape compartment displays the activity nodes and edges. (ibm.com)
Antibodies1
  • The immunoreactivity levels of well-characterized antibodies, for all major retinal cells and their compartments, were obtained using our established semiquantitative confocal and imaging techniques. (molvis.org)
Tissue2
  • The Cell Compartment Kit is designed for fast and easy subcellular fractionation of intact eukaryotic cells and tissue. (qiagen.com)
  • and/or metabolism being allowed in each tissue compartment. (cdc.gov)
Synthesis1
  • The homeostatic regulation of cellular ATP is achieved by the coordinated activity of ATP utilization, synthesis, and buffering. (molvis.org)
System4
  • Accordingly, in CKD, bone mineral mobilisation would serve as a buffer system to acid accumulation. (cambridge.org)
  • We propose that compartmentalized biosynthetic reactions are sensitive to the size of the compartment, likely due to scaling-dependent changes within the system, such as enzyme packing density. (biorxiv.org)
  • This reaction continues to move to the left as long as CO 2 is constantly eliminated or until HCO 3 - is significantly depleted, making less HCO 3 - available to bind H + . That HCO 3 - and PaCO 2 can be managed independently (by kidneys and lungs, respectively) makes this a very effective buffering system. (medscape.com)
  • Its single buffer system can recover DNA from enzymatic reactions, impure extractions, library preparations, and other sources. (integra-biosciences.com)
Cells3
  • The augmentation of intracellular pH, when cells are in a HEPES buffer, should be taken into consideration in studies that encompass all proton-coupled transporter families. (aspetjournals.org)
  • Our goals were twofold: 1) to characterize the gene expression, protein expression, and activity of key synthesizing and regulating enzymes of energy metabolism in the whole mouse retina, retinal compartments, and/or cells and 2) to provide an integrative analysis of the results related to function. (molvis.org)
  • Expression of nucleoside diphosphate kinase, mitochondria-associated adenylate kinase, and several mitochondria-associated creatine kinase isozymes was highest in the outer retina, whereas expression of cytosolic adenylate kinase and brain creatine kinase was higher in the cones, horizontal cells, and amacrine cells indicating the diversity of ATP-buffering strategies among retinal neurons. (molvis.org)
Product1
  • This product is listed as a Compartment Protein Extraction Kit on their site. (biochain.com)
Activity1
  • As the following figure illustrates, an alternative is to display the partition names in the name compartment of the activity node. (ibm.com)
Major1
  • A major life science industry leader currently has ordered a large volume of the CNMCS buffers for their kit. (biochain.com)
Size1
Relationship1
  • New insights into cell-specific and compartmental ATP and GTP production, as well as utilization and buffering strategies and their relationship with known retinal and cellular functions, are discussed. (molvis.org)
Lower3
  • Lower panels show western blots prepared after cell lysates were processed using the Qproteome Cell Compartment Kit. (qiagen.com)
  • Induction of anesthesia is particularly challenging in the setting of trauma, both because the extent of the patient's injuries is often undetermined or underappreciated (as when there is significant blood loss in closed compartments such as the retroperitoneal space or the lower extremities) and because comorbid conditions frequently are unknown and unoptimized. (medscape.com)
  • It courses down the lateral compartment of the lower leg along with the peroneus longus muscle and the peroneus brevis muscle. (medscape.com)
Model1
Body1
  • Thus, buffers work as a first-line of defense to blunt the changes in pH that would otherwise result from the constant daily addition of acids and bases to body fluids. (medscape.com)
Partition2
  • In this example, the contents of the name compartment show that the opaque action is contained in two partitions: Partition and Partition 2. (ibm.com)
  • Diazinon is found in all environmental compartments, but shows no pronounced tendency to partition to a particular environmental medium. (cdc.gov)
Intracellular Fluid Compartment1
Extracellular fluid compartment1
  • During the last two decades, microdialysis has become a well-established method for continuously sampling substances within the extracellular fluid compartment of various tissues outside the central nervous system, including subcutaneous adipose tissue, dermis, muscle, and other organs (3-6). (medicaljournals.se)
Body fluid compar1
Subcellular4
  • Subcellular annotation of the proteins identified in each fraction demonstrated a significant enrichment of cytoplasmic, cell membrane, and nuclear proteins in the three respective buffer system fractions. (nih.gov)
  • We conclude that DDF can be applied to frozen tissue to generate reproducible proteome coverage discriminating subcellular compartments. (nih.gov)
  • The Cell Compartment Kit is designed for fast and easy subcellular fractionation of intact eukaryotic cells and tissue. (qiagen.com)
  • Electron probe microanalysis of the subcellular compartments of bovine adrenal chromaffin cells. (nih.gov)
Gaylord Archival1
  • Keep your holiday ornaments safe and organized in our Gaylord Archival® Clear Lid 28-Compartment Ornament Box. (gaylord.com)
Lysis2
  • 8. Wash once with ~20mL lysis buffer. (nih.gov)
  • 10. After lysis, wash out with buffer until it runs clear. (nih.gov)
Sequentially2
  • Frozen kidneys were sequentially extracted with three different buffer systems. (nih.gov)
  • The 4 Extraction Buffers are added sequentially to a cell pellet and the respective fractions are isolated by centrifugation (see flowchart ). (qiagen.com)
Tissues2
  • Differential detergent fractionation (DDF) is frequently used to partition fresh cells and tissues into distinct compartments. (nih.gov)
  • The kit contains 4 Extraction Buffers which enable the sequential isolation of proteins associated with the cytosol, membranes, nucleus, and cytoskeleton from cell lysates or tissues. (qiagen.com)
Proteins7
  • Biological membranes are impermeable to magnesium (and other ions), so transport proteins must facilitate the flow of magnesium, both into and out of cells and intracellular compartments. (wikipedia.org)
  • Correlation coefficients across the three fractions were significantly lower than those for the replicates, supporting the capability of DDF to differentially fractionate proteins into separate compartments. (nih.gov)
  • This demonstrates the feasibility of analyzing cellular compartment-specific proteins in archived tissue samples with the simple DDF method. (nih.gov)
  • By sequential addition of different extraction buffers to a cell pellet, proteins in the different cellular compartments can be selectively isolated. (qiagen.com)
  • Celis buffer (45 min) extracted proteins from all three major compartments. (duke.edu)
  • However, a 20-s extraction in Celis buffer enriched for several proteins and enabled the identification of several novel peptides by mass spectrometry. (duke.edu)
  • Comparison of biotinylated proteins extracted by each method showed that the major vectorially labeled proteins solubilized by Celis buffer were also solubilized by TX-100, TX-114, and 1 M NaCl. (duke.edu)
Battery6
  • The buffer tube acts as the battery compartment and it is not a big space. (popularairsoft.com)
  • When I used this, I installed a 7.4v nunchuck lipo battery and was able to get the batteries inside the stock or buffer tube but there was no space to for the wires to go in. (popularairsoft.com)
  • I did wonder why this was the case as I have used AEGs in the past with the same battery compartment design and I was able to get everything inside. (popularairsoft.com)
  • The MOSFET is attached to one of the wires going into the battery and this obviously is occupying some of the space inside the buffer tube. (popularairsoft.com)
  • The bottom of the pedal is removable with one screw revealing the battery compartment and a pair of DIP switches. (audiogeekzine.com)
  • A later revision to this scanner appeared in late 1992, called the AR-1500 E . This model differs from the earlier model by including a cascade filter at 58.075 MHz IF to improve selectivity, a DC input protection diode to guard against reverse polarity connection, additional buffering in the microprocessor to protect against software crashes when scanning an empty bank, and a reset switch in the battery compartment. (rigreference.com)
Distinct1
  • Each of these compartments has unique barriers with distinct physio-chemical properties. (pathwaymedicine.org)
Diffusion1
  • We used a time-dependent model to simulate Ca 2+ influx, three-dimensional buffered Ca 2+ diffusion, and the binding of Ca 2+ to the release sensor. (jneurosci.org)
Isolation1
  • Following routine isolation in isotonic sucrose buffer, granule K and Cl- had decreased while granule Na+ increased. (nih.gov)
Tubes2
  • Stocks are based on the Type D pattern, without a trapdoor compartment, and fit onto A-1 length buffer tubes. (thetruthaboutguns.com)
  • Magpul SL-K - Drop-in replacement buttstock for AR15/M4 Mil-Spec carbine buffer tubes, the SL-K is designed for compact PDW style weapons or users desiring a small and light stock. (mountsplus.com)
Shelf1
Concentration2
  • Diffusible compounds within the extracellular compartment of the skin diffuse by the concentration gradient across the dialysis membrane into the lumen of the microdialysis probe, and the compounds can be collected in the fluid at the outlet (the dialysate). (medicaljournals.se)
  • Water distribution depends primarily on the concentration of electrolytes in the body compartments and sodium (Na+) plays a major role in maintaining physiologic equilibrium. (who.int)
Samples2
  • Contact angle decreased substantially upon target amplification in both buffer and whole blood samples. (nih.gov)
  • Honey and methadone samples were diluted in sterile phosphate-buffered saline and cultured with or without heat pretreatment (90°C, 10 min) under aerobic and anaerobic conditions at 37°C for 3-4 days by using Columbia blood agar and MYPGD (Mueller-Hinton broth, yeast extract, potassium phosphate, glucose, pyruvate) agar. (cdc.gov)
Solution2
  • Buffering the solution helps reduce the pain of local lidocaine injection. (medscape.com)
  • As part of routine laboratory procedure, horizontal surfaces had been cleaned with a buffered bleach solution (1:10 dilution) at the end of each day. (cdc.gov)
Buttstock1
  • The problem here is that the end of the buttstock has the end of the buffer tube exposed. (popularairsoft.com)
Probe1
Separate1
  • Switchable internal lighting - Separate corridor and compartment lighting, with optional DCC control. (dapol.co.uk)
Systems1
  • The human body is a marvel of biological engineering, with systems and compartments working in harmony to ensure our survival. (pathwaymedicine.org)
Shutter1
  • in Continuous drive mode you can just keep firing off the shots either by holding the shutter release down or pumping it (until the internal buffer is full - see below), however in Single Shot drive mode 'BUSY' is displayed on the status LCD in the viewfinder and on the top LCD to indicate the camera is busy flushing its buffers before you can take the next shot. (dpreview.com)
Model2
  • Quantitation of skeletal-muscle necrosis in a model compartment syndrome. (medscape.com)
  • All Star Model RP-6-EN Single Door Cooler, S.N. 401011. (georgeauction.com)
Pressure3
  • Excessive deep infiltration may falsely elevate the compartment pressure reading. (medscape.com)
  • 3. Tighten screws on pressure compartment. (nih.gov)
  • In this technique the atrium is removed and a water filled balloon is inserted into the left ventricular compartment to measure the pressure developed during cardiac contraction. (omicsonline.org)
Storage1
  • Discussion: As a storage compartment for lipophilic POPs, AT plays a critical role in the toxicokinetics of a variety of drugs and pollutants, in particular, POPs. (nih.gov)
Controls2
  • The pedal has stereo in/out, buffered or true-bypass and controls for FX level, Decay, feedback and mode plus a 3 way toggle to divide the timing. (audiogeekzine.com)
  • Kit boxes are easily customized with inserts and compartments to contain cassettes, reagents, buffers, controls, microplates and other assay components. (boxcoop.com)
Previous1
  • We infer the topography of release sites by comparing simulated transmitter release with previous experimental data (effects of added exogenous Ca 2+ buffers BAPTA and EGTA as well as effects of altered Ca 2+ channel gating). (jneurosci.org)
Addition1
  • In addition to its buffering function, AT is also a target of POPs and may mediate part of their metabolic effects. (nih.gov)
Helps1
  • The first switch activates the buffer circuit which helps keep the signal strength consistent in a large pedal board. (audiogeekzine.com)
Volume2
  • These zero net displacement probabilities measure the mean compartment (pore) volume and cross- sectional area in distributions of isolated pores irrespective of the pore shape. (nih.gov)
  • Let's explore how these compartments are defined, the nature of their separating barriers, and the volume each compartment holds in a healthy individual. (pathwaymedicine.org)
Hours1
  • The heart displays intrinsic rhythmic contractions and can be maintained for hours when perfused with oxygenated Krebs-Hensleit, or comparable, buffer. (omicsonline.org)
Heat1
Choice1
  • 1. Resuspend cell pel et in 4 volumes of the buffer of your choice. (nih.gov)
Circuit1
  • The device is also equipped with Morley's new custom MQ2 Inductor and True Tone buffer circuit that prevent tone suck and signal loading. (guitar.com)
Weight1
  • Granule Na+ and K+ concentrations ranged from 43 to 12 mmol/kg and 28 to 60 mmol/kg dry weight, respectively, depending on the Na+ and K+ content of the buffer. (nih.gov)
Make1
  • This is why artificial insulators make use of air compartments, such as double-pane glass windows which are used for cutting heating bills. (universetoday.com)
Foot1
  • Frink M, Hildebrand F, Krettek C, Brand J, Hankemeier S. Compartment syndrome of the lower leg and foot. (medscape.com)
Data1
  • The socket buffer, or 'SKB', is the most fundamental data structure in the Linux networking code. (nails-beauty.de)
Balance1
  • Maintaining the right balance of fluids in each compartment is crucial for our body's optimal functioning. (pathwaymedicine.org)