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Microscopy, ConfocalMicroscopyMicroscopy, ElectronMicroscopy, Electron, ScanningMicroscopy, FluorescenceMicroscopy, Electron, TransmissionMicroscopy, Atomic ForceMicroscopy, ImmunoelectronMicroscopy, Fluorescence, MultiphotonOphthalmic NerveFluorescent DyesMicroscopy, VideoGreen Fluorescent ProteinsCells, CulturedCryoelectron MicroscopyCell MembraneMicroscopy, Scanning TunnelingFluorescent Antibody TechniqueCell LineMicroscopy, InterferenceReagent Kits, DiagnosticFluorescent Antibody Technique, IndirectCorneaImmunohistochemistryMicroscopy, Electron, Scanning TransmissionMicroscopy, Phase-ContrastLuminescent ProteinsCorneal StromaProtein TransportStaining and LabelingMicroscopy, PolarizationCell NucleusTime FactorsMicroscopy, Scanning ProbeMolecular Sequence DataImage Processing, Computer-AssistedBlotting, WesternXanthenesActinsCytoplasmSensitivity and SpecificityImaging, Three-DimensionalCalciumCattleRabbitsEpithelium, CornealCorneal DiseasesEndocytosisFlow CytometrySwineFluorescein-5-isothiocyanateRecombinant Fusion ProteinsProtein BindingAmino Acid SequenceSubcellular FractionsMembrane ProteinsRats, Sprague-DawleyEndothelium, CornealEpithelial CellsTransfectionLasersSurface PropertiesMicrotomyCell CountMice, Inbred C57BLFluorescenceCytoskeletonBiological TransportCell Line, TumorRhodaminesCarbocyaninesReproducibility of ResultsNanoparticlesHeLa CellsParticle SizeEquipment DesignModels, BiologicalRats, WistarRNA, MessengerMicrotubulesMicroscopy, AcousticCalcium SignalingSignal TransductionEnzyme-Linked Immunosorbent AssayApoptosisGolgi ApparatusMitochondriaAniline CompoundsLasers, ExcimerTissue DistributionFluoresceinReverse Transcriptase Polymerase Chain ReactionEndoplasmic ReticulumEndosomesFluoresceinsCell SurvivalTime-Lapse ImagingCricetinaeCercopithecus aethiopsDisease Models, Animal
FluorescenceMicroscopesRaman MicroscopyElectron microscopySpecimensDeconvolutionSTEDMETHODSCLSMVivoImagingLaserStructuresTechniquesTissueLasersExcitationIntracellularImagesMeasurementSuitableSilicaExperimentalSystemsEvaluateApplicationsClinicalHigh-resolutionResolutionProductsTechnologyResearchPlatformARTICLECompletelyStandardTest
Fluorescence17
  • This includes imaging in reflection mode and TIRF in addition to Nomarski, epi-fluorescence, and fluorescent confocal. (nabble.com)
  • Octopus offers a range of imaging techniques including several modes of multidimensional single molecule microscopy (particle kinetics and dynamics in live cells via single particle tracking colocalisation, single particle FRET and single particle polarisation, and structure determination in fixed cells - at ~5 nm resolution via fluorescence localisation with photobleaching (FLimP)), super-resolution microscopy (STORM, PALM, SIM, STED), and confocal microscopy (FLIM, FRET, and multiphoton). (wayforlight.eu)
  • Confocal fluorescence microscopy was utilized to analyze the silica surface at different stages of surface modification and examine the passivation of the substrate to the formation of untethered lipid bilayers. (aiche.org)
  • The Principles of Fluorescence Techniques workshop will outline the basic concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. (fluorescence-foundation.org)
  • The lectures will be complemented by the direct utilization of steady-state and lifetime fluorescence instrumentation and confocal microscopy for FLIM and FFS applications. (fluorescence-foundation.org)
  • The lasers commonly employed in optical microscopy are high-intensity monochromatic light sources, which are useful as tools for a variety of techniques including optical trapping, lifetime imaging studies, photobleaching recovery, and total internal reflection fluorescence. (fsu.edu)
  • In addition, lasers are also the most common light source for scanning confocal fluorescence microscopy, and have been utilized, although less frequently, in conventional widefield fluorescence investigations. (fsu.edu)
  • Illustrated in Figure 1 is a self mode-locked Ti:sapphire pulsed laser, which is currently one of the preferred laser excitation sources in a majority of multiphoton fluorescence microscopy investigations. (fsu.edu)
  • We propose an alternate minimization algorithm for estimating the point-spread function (PSF) of a confocal laser scanning microscope and the specimen fluorescence distribution. (inria.fr)
  • 1 - Blind deconvolution for diffraction-limited fluorescence microscopy . (inria.fr)
  • Optical Sections of biological samples obtained from a fluorescence Confocal Laser Scanning Microscopes (CLSM) are often degraded by out-of-focus blur and photon counting noise. (inria.fr)
  • The transfection efficacies noticed in terms of EGFP expression levels using flow cytometry were well corroborated using independent fluorescence microscopy studies. (iisc.ac.in)
  • This could be correlated with the effects of Ag NPs on mitochondrial dysfunction, as determined by confocal fluorescence microscopy and JC-1, an indicator of mitochondrial permeability. (sigmaaldrich.com)
  • Volvox, a green algae living in fresh water ponds viewed by different kinds of microscope illumination a) bright-field b) dark-field c) phase contrast d) differential Interference contrast e) Rheinberg lighting f) fluorescence microscopy with green excitation. (canadiannaturephotographer.com)
  • Scanning Electron Microscopy (SEM) and Confocal Fluorescence Microscopy (CFM) was used to characterize the presence of insecticide on the surface of the monofilaments. (biomedcentral.com)
  • This annual workshop outlines fundamental concepts of fluorescence techniques and the successful utilization of the currently available commercial instrumentation. (iss.com)
  • Confocal fluorescence microscopy images of phospholipid bilayers supported by gallium phosphide nanowires. (lu.se)
Microscopes7
  • Such holders are readily available and can fit into most commercial electron microscopes thereby realizing the SCEM mode. (wikipedia.org)
  • From the first confocal microscope we used in 1991 through many other microscopes over the years, we have not had problems with planapochromat lenses with the image being in focus corner to corner across the field. (nabble.com)
  • This includes newer microscopes with wider fields of view and a spinning disk confocal with a Cairn twin camera setup. (nabble.com)
  • The SCOPE offers a combination of commercial and custom-built microscopes, allowing users to address an extensive array of biological questions. (umassmed.edu)
  • With development of a handheld confocal microscopes, researchers at the University of Arizona Health Sciences want to make existing technology more affordable and accessible to a larger number of patients. (arizona.edu)
  • Bright-field microscopy is the standard form of illumination that most light microscopes offer and the least expensive mode. (canadiannaturephotographer.com)
  • The premises and equipment of the Department of Biology are modern and have almost everything a biologist needs, from molecular laboratories, and electron and confocal microscopes to a field station, and wind tunnels. (lu.se)
Raman Microscopy3
Electron microscopy8
Specimens3
  • Confocal laser scanning microscopy is a powerful and popular technique for 3D imaging of biological specimens. (inria.fr)
  • High-temperature laser-scanning confocal microscopy (HT-LSCM) has proven to be an excellent experimental technique through in situ observations of high temperature phase transformation to study kinetics and morphology using thin disk steel specimens. (edu.au)
  • Bright-field microscopy also works well with living specimens that can be stained with vital dyes or arthropods that have a thin transparent cuticle made of chitin. (canadiannaturephotographer.com)
Deconvolution1
  • 2 - Richardson-Lucy Algorithm with Total Variation Regularization for 3D Confocal Microscope Deconvolution . (inria.fr)
STED1
  • ATTO ® 488, ATTO ® 647N and ATTO ® 594 dyes are suitable for St imulated E mission D epletion ( STED ) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. (sysy.com)
METHODS6
  • METHODS: Confocal microscopy was utilized to generate CAD models of unused and explanted catheters. (wayne.edu)
  • The most common methods include temporally scrambling laser light by rapidly varying the optical path length between the light source and the microscope, or scanning the specimen point by point as is the case in confocal microscopy systems. (fsu.edu)
  • 23 - Wavelet-based restoration methods: application to 3D confocal microscopy images . (inria.fr)
  • Confocal and multiphoton imaging techniques are still the methods of choice for performing sophisticated studies on biological samples. (leica-microsystems.com)
  • All the efficient co-liposomal formulations derived from each of these geminis and a helper lipid, dioleoyl phosphatidyl ethanolamine (DOPE) were well characterized using physical methods such as atomic force microscopy (AFM) and dynamic light scattering (DLS). (iisc.ac.in)
  • Current commercial wall linings are produced using labour intensive fabric weaving methods. (biomedcentral.com)
CLSM1
  • Confocal laser scanning microscopy (CLSM) was used to evaluate qualitatively the enamel morphology. (bvsalud.org)
Vivo1
  • Multiphoton confocal microscopy uses a focused beam of photons to provide high-resolution images of tissue in vivo at depths up to 600 μm. (warf.org)
Imaging9
  • 3D imaging in SCEM was expected from the confocal geometry of SCEM, and it has recently been confirmed by theoretical modeling. (wikipedia.org)
  • Ready for a completely new confocal imaging system optimized for large mosaic image acquisition? (vivascope.de)
  • The UMass Chan Medical School light microscopy core facility, the Sanderson Center for Optical Experimentation (SCOPE) , offers the tools and expertise required to perform a wide variety of quantitative microscopy techniques including super resolution imaging, multi-photon intravital and live tissue imaging, confocal microscopy and high-speed time-lapse imaging. (umassmed.edu)
  • The OCTOPUS (Optics Clustered to OutPut Unique Solutions) imaging cluster offers a range of advanced imaging techniques including multidimensional single molecule microscopy, multiphoton, confocal microscopy and optical tweezers. (wayforlight.eu)
  • This is an advanced imaging facility containing a mixture of in house-built and commercial systems operating in partnership with its sister facility ULTRA. (wayforlight.eu)
  • Described as a "portable confocal microscope" (PCM), the device is a modernized and more practical use of reflective confocal microscopy (RCM) - technology that provides non-invasive imaging of the skin. (arizona.edu)
  • The Wisconsin Alumni Research Foundation (WARF) is seeking commercial partners interested in developing a system that corrects for optical distortion to enable higher resolution imaging of structures deep within tissue. (warf.org)
  • 1 - Blind deconvoltion for thin layered confocal imaging . (inria.fr)
  • Two main PA imaging techniques are used: PA tomography (PAT) and PA microscopy (PAM) [ 2 , 6 , 9 ]. (e-ultrasonography.org)
Laser4
  • The anterior cornea of four human volunteers was examined by an in-house developed confocal laser scanning microscope based on a commercial available instrument (Heidelberg Retina Tomograph II, Heidelberg Engineering GmbH, Germany). (arvojournals.org)
  • This website is specifically aimed at doctors who are interested in becoming confident and independent in the recognition and application of our VivaScope products using confocal laser scanning microscopy and who wish to deepen their knowledge. (vivascope.de)
  • The coherence of monochromatic light produced by most laser systems introduces problems in the application of these light sources for classical widefield microscopy. (fsu.edu)
  • For visualization of viable and dead cells under a confocal laser scanning microscope, the follicles were stained with Calcein AM and ethidium homodimer-1. (biomedcentral.com)
Structures2
  • Confocal microscopy is used to view biological tissue through intervening tissues or structures. (warf.org)
  • Coherent Anti-Stokes Raman Scattering) microscopy is a dye-free method which images structures by displaying the characteristic intrinsic vibrational contrast of their molecules. (leica-microsystems.com)
Techniques1
  • The goal is to bring together leading experts in the field of flows of soft materials, with a particular focus on neutrons and X-rays, but also complementary techniques including NMR, confocal microscopy, theory and computer modeling. (lu.se)
Tissue1
  • Our confocal microscopy-based solution enables time-efficient differentiation between pathogenic and healthy tissue in real time and on-site, in your practice or surgery room. (vivascope.de)
Lasers2
  • As the variety of commercial lasers increases, users need a greater overall understanding of how to measure their output. (photonics.com)
  • The commercial insturment we purchased has three lasers, which allows us to expand into studies evaluating fluorescent properties," Dr. Curiel said. (arizona.edu)
Excitation1
  • The problem is that the confocal volume sensibly depends on numerous parameters of the optical set-up, refractive index mismatch of the sample solution and the objective's immersion medium, coverslide thickness variations and especially the dependence on optical saturation of the fluorescent dye, which can occur at even very low excitation powers. (picoquant.com)
Intracellular1
  • Transfection efficacies were also analyzed in terms of the degree of intracellular delivery of labeled plasmid DNA (pDNA) using confocal microscopy which revealed an efficient internalization in the presence of serum. (iisc.ac.in)
Images2
  • Dr. Clara Curiel Lewandrowski in front of a screen showing the images produced by a confocal microscope. (arizona.edu)
  • Although confocal microscopy images are much sharper than standard epifluorescence ones, they are still degraded by residual out-of-focus light and by Poisson noise due to photon-limited detection. (inria.fr)
Measurement1
  • Thorlabs' new Mini AO Kits enable real-time, high-precision wavefront measurement and correction, and are designed to be quickly integrated within commercial and research systems. (prlog.org)
Suitable1
Silica1
  • Currently, the microabrasive agents used are 6.6% hydrochloric acid with silica, in a commercial presentation, or pumice added to 35% phosphoric acid, with mechanical application under low-speed rotation 5 . (bvsalud.org)
Experimental1
  • The model is validated against a commercial finite element analysis software package, strand7, and optimized with experimental data obtained under near-to equilibrium conditions. (edu.au)
Systems2
  • On one hand we present FoCuS-scan, scanning FCS software that provides an end-to-end solution for processing and analysing scanning data acquired on commercial turnkey confocal systems. (abberior.rocks)
  • Melioidosis presents diagnostic challenges because it causes nonspecific clinical signs, serologic screening can produce unreliable results, and culture isolates are often misidentified on rapid commercial testing systems. (cdc.gov)
Evaluate1
  • To quantitatively evaluate a FCS experiment, one has to exactly know the shape and the size of the confocal volume. (picoquant.com)
Applications2
  • When combined with the potential applications in commercial avionics, electronics and medical hole drilling applications (secondary markets) the potential cost savings are estimated at ~$2B over the next thirty years. (sbir.gov)
  • In applications such as materials processing, environmental monitoring and confocal microscopy , understanding the laser's performance is critical. (photonics.com)
Clinical1
  • Thanks to funding from the UArizona Technology and Research Initiative Fund (TRIF) , Dr. Curiel purchased a commercial instrument for her clinical practice in 2014. (arizona.edu)
High-resolution1
  • ATTO ® 488 and ATTO ® 594 are also recommended for PALM and dSTORM high resolution microscopy. (sysy.com)
Resolution1
  • His first scanning confocal electron microscope demonstrated the 3D properties of the SCEM, but have not realized the sub-nanometer lateral spatial resolution achievable with high-energy electrons (lateral resolution of only ~80 nm has been demonstrated). (wikipedia.org)
Products2
  • The low density and small size of these biologically persistent particles makes respiratory exposures to workers likely during the production or use of commercial products. (cdc.gov)
  • Confocal microscopy demon- exposures to workers likely during the production strated nanotubes within the nucleus that were in or use of commercial products. (cdc.gov)
Technology1
  • Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation. (sysy.com)
Research3
  • Microscopy Research Technique , 69: pages 260-266, April 2006 . (inria.fr)
  • Prior to joining Pfizer, Kevin worked at GlaxoSmithKline (GSK) and, in addition to leading the formation of multiple strategic commercial and academic partnerships, he led epigenetics research and was responsible for the creation of the EpiNova Discovery Performance Unit. (macomics.com)
  • We monitor the development of new technologies and devices for the commercial market and identify components that might be interesting for research in biology. (lu.se)
Platform1
  • A solution for commercial platform PPMS, Cryogenics, Oxford and NanJing PengLi. (multi-field.com)
ARTICLE2
  • This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/3.0/ ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. (e-ultrasonography.org)
  • I will present a separate article on dark-field microscopy in the future. (canadiannaturephotographer.com)
Completely1
  • The shape of an autocorrelation curves is completely determined by the confocal volume(s), whereas the shape of the cross-correlation curve is also dependent on the overlap of the two foci. (picoquant.com)
Standard1
  • HEK 293T, HeLa and Caco-2 significantly better than a potent commercial standard Lipofectamine 2000 (L2K) both in the absence and presence of serum (FBS). (iisc.ac.in)
Test2
  • Drs. Clara Curiel Lewandrowski and Dongkyun Kang prepare to do a side-by-side test of the confocal microscope and the portable unit to compare the image quality. (arizona.edu)
  • The bacterial isolate from the abscess was initially misidentified on a commercial test. (cdc.gov)