Colony forming units assay. Medical search. Frequent questions

Biofilm formation was assessed by cresyl violet assay, quantification of colony-forming units and scanning electron microscopy, and atomic force microscopy. (wur.nl)
The novel DNA extraction protocol and specific E. coli K12 assay are sensitive and robust enough for detection and quantification within iron drinking water pipe biofilms, and are particularly well suited for studying enteric bacterial interactions within biofilms. (who.int)

Burst forming unit-erythroid colony formation inhibition assay suggesting possible erythroid suppression mechanism. (bvsalud.org)

To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
and (iii) Reg4 significantly increases colorectal cancer and pancreatic cancer cell proliferation and their clonogenic potential in stem cell assays. (wustl.edu)

7 They used in vitro assays and a human wound skin model to assess whether blue light therapy reduces the P. aeruginosa burden and identified the specific intensities and treatment times needed to maximize antibacterial effects. (the-scientist.com)
In vitro assays that permit cloning of tumour cells in soft agar have been improved during the last 5 years. (tno.nl)

This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test. (who.int)

We report the results of a laboratory sensitivity and specificity evaluation of the Rapid Analyte Measurement Platform (RAMP) Dengue Virus (DENV) antigen detection assay, which is designed to detect all serotypes of DENV in mosquito pools. (cdc.gov)

Because of uncertainties regarding the comparability of granulocyte-macrophage and granulocyte colony-stimulating factors with regard to their effects on mature neutrophils (PMNs), we compared the actions of the two cytokines on reactive oxidant production and granular secretion by these cells. (uhi.ac.uk)

Circulating hematopoietic progenitor cells giving rise to colonies containing basophilic cells (basophilic cell colony-forming units in culture [CFU-c]), nasal epithelial metachromatic cells (basophils and/or mast cells) (NMC), and blood basophils were enumerated before, during, and after a ragweed-pollen season in patients with ragweed hayfever and patients with perennial allergic rhinitis who were not ragweed allergic. (mcmaster.ca)

The ability to use autologous dental progenitor cells (DPCs) to form organized periodontal tissues on titanium implants would be a significant improvement over current implant therapies. (nih.gov)
STUDY DESIGN AND METHODS: The hematopoietic progenitor cell (HPC) and lymphocyte subset (LS) content of approximately 8000 CB units were characterized, and these results were correlated with donor ethnicity, birth weight, gestational age, sex, and type of delivery. (emmes.com)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
Moreover, treatment with rhGH increased the frequency of hematopoietic stem/progenitor cells as measured by flow cytometry and colony forming unit assays in bone marrow harvested at day 14 after irradiation, suggesting the effects of rhGH are at the hematopoietic stem/progenitor level. (duke.edu)

One study found that the number of colony-forming units (CFUs) was found to be a stronger independent predictor of engraftment and a potential better option in assessing the potency of a cord blood unit for transplantation. (cryo-cell.com)
Furthermore, the antimicrobial abilities evaluated in vitro with Streptococcus mutans ( S. mutans ), Fusobacterium nucleatum ( F. nucleatum ) and Candida albicans ( C. albicans ) using the live/dead, MTT and colony-forming units (CFUs) assay. (peerj.com)

The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
This property forms the basis of in vivo assays of HSC function. (stemcell.com)
The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)

There are currently several methods you can use to assess the potential future effectiveness of your child's cord blood collection including the collection's volume (in milliliters), the number of total cells (TNC count), the number of stem cells (CD34+ count), and a measurement of the stem cell functionality and potency (such as a colony-forming unit [CFU] count). (cryo-cell.com)
During this time, the cells will begin to form colonies. (cryo-cell.com)
The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)
It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)
Colony-forming unit assays yielded denser cell colonies in the HA treated samples compared to the controls, yet no difference in the number of colonies was detected, indicating that the number of stem cells remained unchanged. (europa.eu)
Antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. (tno.nl)
The experiments presented here suggest that the adherent cells involved in this phenomenon are the progeny of bone marrow derived fibroblastoid colony forming units. (tno.nl)
After incubation, cells will be plated for CFU-GM and CFU-Leuk assays to determine survival of committed hematopoietic progenitors and log-kill of AML cells, and optimal conditions for antileukemic effect with stem-cell sparing. (arizona.edu)

The researchers used colony-forming unit assays to track the number of bacteria growing in suspension in vitro after blue light treatment. (the-scientist.com)
Gram negative bacteria (GNB) were analyzed by plating and counting colony forming units. (cdc.gov)
Consequently, this propolis underwent fractionation by chromatographic methods monitored through Agar-diffusion assays with these bacteria, which resulted in the isolation and identification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which showed MIC of 62.5 and 250 µg/mL against B. subtilis and S. aureus, respectively. (scielo.br)
For example, a single-well assay has been developed that uses five molecular beacons to detect mutations associated with rifampin resistance in M. tuberculosis bacteria and appears to perform similarly as the line-probe assays. (cdc.gov)

It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)

Kits for detecting mutations associated with rifampin resistance that are commercially available in Europe and elsewhere include line-probe assays (INNO-LiPA® Rif.TB, Innogenetics and GenoType® MTBDR( plus ), Hain LifeScience GmbH) and microarray assays (CombiChip Mycobacteria DR, GENE IN). (cdc.gov)
For the hybridization assays, the region of the target gene associated with resistance is PCR amplified, and the labeled PCR products hybridized to oligonucleotide probes immobilized on a nitrocellulose strip or in a microarray. (cdc.gov)

5.2 The purpose of this test method is to direct a user in how to grow, treat, sample and analyze a Pseudomonas aeruginosa biofilm using the MBEC Assay. (astm.org)
1.1 This test method specifies the operational parameters required to grow and treat a Pseudomonas aeruginosa biofilm in a high throughput screening assay known as the MBEC (trademarked) 2 (Minimum Biofilm Eradication Concentration) Physiology and Genetics Assay. (astm.org)
Biofilm population density is recorded as log 10 colony forming units per surface area. (astm.org)
The biofilm detection limit was 10(3) colony-forming units (CFU) E. coli K12 mL(-1), but required a modified protocol, which included a bio-blocker Pseudomonas aeruginosa with ethylenediaminetetraacetic acid buffered to pH 5 prior to cell lysis/DNA extraction. (who.int)

Together they form a unique fingerprint. (uhi.ac.uk)

and today is the most widely used in vitro assay for HSPCs. (stemcell.com)

Antibacterial activity of the prepared sample was assessed by determining the number of colony-forming units per milliliter (CFU/ml) using a standard viable cell count assay. (nature.com)

Besides, EA extract significantly inhibited cell migration and colony formation at 250 to 1000 Âµg/ml concentration. (jmbfs.org)

For the INNO-LiPA Rif.TB assay, the pooled sensitivity was 0.97 (95%CI 0.95-0.98) and the pooled specificity was 0.99 (95%CI 0.98-1.00) for detecting rifampin resistance in M. tuberculosis isolates. (cdc.gov)
For the MTBDR and MTBDR( plus ) assays, the pooled sensitivity was 0.98 (95%CI 0.96-0.99) and the pooled specificity was 0.99 (95% CI 0.97-0.99) for detecting rifampin resistance in isolates or directly from clinical specimens. (cdc.gov)
Cefoxitin (30g) was used as surrogate to determine phenotypic methicillin resistance in staphylococcus isolates, and the methicillin resistance ( mec A) gene was detected by conventional PCR assay. (who.int)

Knockout of CD19 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR807388_LV) and LentiArray Cas9 Lentivirus (Product # A32064). (thermofisher.com)

1.5 Basic microbiology training is required to perform this assay. (astm.org)

The RAMP DENV assay was able to detect geographically distinct strains of all 4 DENV serotypes in virus-spiked mosquito pools that contained at least 4.3 log10 plaque forming units/ml, although discrete sensitivity limits varied slightly for each serotype. (cdc.gov)

Although the specific cDNA amplification mechanisms of reverse-transcriptase polymerase chain reaction (RT-PCR) and RT loop-mediated isothermal amplification (RT-LAMP) are very different, both molecular assays serve as options to detect arboviral RNA in mosquito pools. (cdc.gov)

Pro-Lab Diagnostics, Inc., Round Rock, Texas) and compared the results to a validated real-time RT-PCR assay. (cdc.gov)
Laboratory generated virus stock samples containing 2.3 log10 plaque-forming units (PFU)/ml and intrathoracically inoculated mosquitoes containing 2.4 log10 PFU/ml produced positive results in the Pro-AmpRT WNV assay. (cdc.gov)

A quantitative polymerase chain reaction assay (115 bp amplicon) specific to Escherichia coli K12 with an ABI(TM) internal control was developed based on sequence data encoding the rfb gene cluster. (who.int)
KPCs) are Ambler class A plasmid- gene by polymerase chain reaction cin, imipenem, ertapenem, meropenem, encoded enzymes that are capable of (PCR) assay provides laboratories trimethoprim/sulfamethoxazole, piper- hydrolyzing all beta-lactam antibiotics, with a means to quickly identify the acillin, piperacillin/tazobactam and including monobactams, extended- presence of this important resistance tobramycin (Oxoid). (who.int)

Investigation of toxicity of Cu-Ti thin films was conducted the using the MTT assay, which revealed their biocompatibility and absence of cytotoxicity, further confirming their potential as promising biomaterials for various applications. (nature.com)
The MTT and LDH assays were performed to evaluate the cytotoxicity effect of the extract and the determination of cell death. (jmbfs.org)

Wound-healing assay and colony formation analysis were employed for migration and proliferation evaluation. (jmbfs.org)
Migration assay To examine the result of bisphosphonates on EPC migration, a 24-well Boyden chamber assay program (ThinCert? (biobender.com)

Additional sex combs-like 1 ( ASXL1 ) mutations have been described in all forms of myeloid neoplasms including chronic myelomonocytic leukemia (CMML) and associated with inferior outcomes, yet the molecular pathogenesis of ASXL1 mutations ( ASXL1 -MT) remains poorly understood. (biomedcentral.com)
Because molecular beacons can use different fluorophores, real-time PCR assays can be designed in which different DNA fragments or mutations can be amplified and detected simultaneously in the same tube. (cdc.gov)

Cultures were incubated at 37C with 5% CO2, and scored for colony formation after 14 days. (biobender.com)

Colony-forming unit assay To examine the effect of the colony-forming ability, EPCs were cultured in MethoCult? (biobender.com)

Assay specificity was evaluated using three E. coli K12 strains (ATCC W3110, MG1655 & DH1), 24 non-K12 E. coli and 23 bacterial genera. (who.int)

In the patients with seasonal hay fever, the following was observed: basophilic cell CFU-c, measured as basophilic cell or histamine-containing colonies, were significantly reduced during the ragweed season compared to before (p less than 0.005) or after (p less than 0.025) the season in the ragweed-allergic group only. (mcmaster.ca)

In vitro, MPL515/630 but not MPL515/625 or MPL515/625/630 retained the ability to induce TPO-independent proliferation and increase colony-forming unit megakaryocytes (CFU-Mk). (biomedcentral.com)
Overall discriminatory ability of the assay was 99% and overall accuracy was 97%, with all studies yielding consistently high performances. (cdc.gov)

The performance of the line-probe assays relative to culture-based DS tests was evaluated in meta-analyses (3-5). (cdc.gov)

Typically, PCR is used to amplify a target sequence followed by a second assay to determine if the sequence contains a mutation associated with resistance. (cdc.gov)

3, 4 The reactor design allows for the simultaneous testing of multiple disinfectants or one disinfectant with multiple concentrations, and replicate samples, making the assay an efficient screening tool. (astm.org)
Water samples were assayed for potential respiratory viruses (adenoviruses and enteroviruses), but none were detected. (cdc.gov)

We determine the TU/mL based upon the p24 assay from ZeptoMetrix. (sigmaaldrich.com)
Finally, a Polymerase Chain Reaction (PCR) assay to determine the most prevalent species of Enterococcus spp. (bvsalud.org)

The assay device consists of a plastic lid with ninety-six (96) pegs and a corresponding receiver plate with ninety-six (96) individual wells that have a maximum 200 μL working volume. (astm.org)

La présente étude menée dans un hôpital de soins tertiaires en Égypte a utilisé la méthode de PCR en temps réel pour évaluer la présence du gène blaKPC dans les isolats de K. pneumoniae non sensibles à l'ertapénème, puis a comparé les résultats à l'aide du test de Hodge modifié. (who.int)

Rate your level of confidence in performing the CFU assay. (stemcell.com)

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Health Care1

Quantification2

Inhibition1

Clonogenic2

Vitro assays2

Real-time PCR1

Antigen1

Granulocyte-macrophage1

Hematopoietic Progenitor Cells1

Progenitor5

CFUs2

Vivo3

Cells9

Bacteria4

Transplantation2

Microarray2

Biofilm4

Unique fingerprint1

HSPCs1

Viable1

Concentration1

Isolates3

Lentiviral1

Microbiology1

Sensitivity1

Detect1

Results2

Gene2

Cytotoxicity2

Migration2

Molecular2

Formation1

MethoCult1

Strains1

Significantly1

Ability2

Culture1

Sequence1

Samples2

Determine2

Volume1

Test1

Rate1