• DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in murine intestinal organoids. (nature.com)
  • Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
  • Intestinal epithelial cells (IECs) have evolved to build a complex physiological barrier that separates the intestinal lumen from the underlying mucosal tissue. (nature.com)
  • In children diagnosed with IBD, genome-wide DNA methylation profiling of purified intestinal epithelial cells clearly distinguishes between healthy individuals and the IBD group 8 . (nature.com)
  • In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
  • However, with the support of dynamic fluid, intestinal epithelial cells can now form a more complex 3D structure that closely resembles the human body. (daxiangbio.com)
  • Here we investigated whether polarized monolayers of intestinal epithelial cells might regulate inflammatory responses by secreting IL-8 in a vectorial fashion (i.e. apical versus basolateral) depending on the location of the TLR stimulus. (biomedcentral.com)
  • The CXCR1 receptor for IL-8 was expressed only on the apical membrane of Caco-2 BBE cells and differentiated epithelial cells in the human small intestine and colon. (biomedcentral.com)
  • These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
  • Intestinal epithelial cells (IECs) play a key role in the inflammatory response to colonizing or invading microorganisms via the secretion of interleukin-8 (IL-8). (biomedcentral.com)
  • Interestingly, IL-8 has been measured in the colon milieu of healthy volunteers using a dialysis bag inserted in the rectum [ 9 ] suggesting that it might be apically secreted by colonic epithelial cells. (biomedcentral.com)
  • Immune Response Signatures of Benzo(α)pyrene Exposure in Normal Human Mammary Epithelial Cells in the Absence or Presence of Chlorophyllin. (boysandgirlsclubofcypress.com)
  • CYP1A1 and CYP1B1 gene expression and DNA adduct formation in normal human mammary epithelial cells exposed to benzo[a]pyrene in the absence or presence of chlorophyllin. (boysandgirlsclubofcypress.com)
  • However, the mechanism by which adenine inhibits the proliferation of cancer cells is sketchy. (hindawi.com)
  • In this study, we investigated the effects of adenine against the proliferation of colon cancer cells. (hindawi.com)
  • RPS3 knockdown led to a significant decrease in the proliferation, survival, migration and invasion and an increase in the apoptosis of Caco-2 cells. (edu.lb)
  • In this study, we determined the effects of the Kigelia africana, the Mimusops zeyheri and the Ximenia caffra seed-oil on cell proliferation in culture. (iiarjournals.org)
  • The suppression of Caco-2 and HEK-293 cell proliferation by K. africana, M. zeyheri and X. caffra seed oils suggest a potential antiproliferative effect of these tree seed oils on the two cell lines. (iiarjournals.org)
  • The objective of this study was to determine the effect of K. africana, M. zeyheri and X. caffra seed oils on Caco-2 and HEK-293 cell proliferation. (iiarjournals.org)
  • 2016). SCFAs assist in gut homeostasis by their immunomodulatory functions as well as by maintaining metabolism, proliferation, differentiation and promotion at low pH, favoring beneficial microbes with the concomitant reduction in pathogen bacterial growth and viability (Nepelska et al. (psychosocialsomatic.com)
  • Intestinal-specific deletion of Pcdh20 damages epithelial cell proliferation and differentiation and causes morphological changes, including shortened crypts and microvilli. (biomedcentral.com)
  • Alteration in fatty acid metabolism is usually a hallmark of cancer and several lines of evidence showed that limiting fatty acid availability controls malignancy cell proliferation 22 23 As fatty acids are required for the formation of membrane components energy sources and the production of cellular signaling molecules during cancer cell proliferation FABPs might play an important role PIK-293 in cellular proliferation. (mdm2-inhibitors.com)
  • Results suggest for the first time that high‐level FABP5 promotes PIK-293 cell proliferation and metastatic potential in CRC cells. (mdm2-inhibitors.com)
  • The effects of the COX-2 inhibitor celecoxib and the 5-LOX inhibitor MK886 on prostaglandin E(2) and cysteinyl leukotriene production,tumor cell proliferation,cell apoptosis,and Bcl-2/Bax expression were evaluated in the Caco-2 and HT29 colon cancer cells. (unifi.it)
  • Combined treatment with 10 micromol/L celecoxib and MK886 could prevent this activation and had additive effects on inhibiting tumor cell proliferation,inducing cell apoptosis,decreasing Bcl-2 expression,increasing Bax expression,and determining mitochondrial depolarization in comparison with treatment with either inhibitor alone. (unifi.it)
  • 2009). Fermentation products also led to improved intestinal monolayer integrity in Caco-2 treated with deoxycholic acid (Vannucci et al. (psychosocialsomatic.com)
  • Trans-epithelial electrical resistance measurements indicate Caco-2 monolayer integrity, and we recorded changes to this integrity following exposure to the fermentation products of selected probiotics and prebiotics, in the form of nondigestible oligosaccharides (NDOs). (northumbria.ac.uk)
  • Using T cell-specific Dusp8 conditional KO (T-Dusp8 cKO) mice, mass spectrometry analysis, ChIP-Seq, and immune analysis, we found that DUSP8 interacted with Pur-α, stimulated interleukin-9 (IL-9) gene expression, and promoted Th9 differentiation. (bvsalud.org)
  • Animal-derived oils and purified fatty acids, but not indigenous fruit-tree-derived seed oils, have been used to study cell growth and differentiation. (iiarjournals.org)
  • IM38 inhibited differentiation into helper T17 cells and reduced IL-17 levels in the colon. (medicine.news)
  • IM38 increased HFD-suppressed differentiation into regulatory T cells and IL-10 levels. (medicine.news)
  • Semaphorin 4D (Sema4D) belongs to the semaphorin family, which binds to receptors such as Pleixn-B1 (transmembrane structure receptor), CD72 (cluster of differentiation antigen 72, mainly expresses on lymphocyte cells), then activates downstream signaling molecules. (jcancer.org)
  • Intestinal differentiation marker proteins Villin, Mucin 2 and glycocalyx were expressed and distributed on the apical side of the colon. (daxiangbio.com)
  • Adenine significantly inhibited the viability of colon cancer cells, HT29 and Caco-2 cells, in a dose-dependent manner. (hindawi.com)
  • Adenine induced significant apoptosis in HT29 cells, whereas Caco-2 cells exhibited less apoptotic responses. (hindawi.com)
  • By means of a recently developed non-invasive ex vivo minimal model based on the interaction of the human enterocyte-like HT29 cell line and fecal slurries, we explored the enterocyte-associated microbiome of 21 Hadza hunter-gatherers and nine urban living Italians. (frontiersin.org)
  • Microbial suspensions obtained from Hadza or Italian stools were first evaluated for structural integrity by high resolution-scanning electron microscopy and co-incubated with HT29 cell monolayers. (frontiersin.org)
  • Effects of chlorophyllin on transport ofdibenzo(a, l)pyrene, 2-amino-1-methyl-6-phenylimidazo-[4,5-b]pyridine,and aflatoxin B(1) across Caco-2 cell monolayers. (boysandgirlsclubofcypress.com)
  • High lipophilicity often leads to high rate of metabolism, poor solubility, high turn-over, and low absorption. (themama.ai)
  • Optimized ARV- SNEP spontaneously formed nanoglobules of 45.02 nm with zeta potential of −3.78 mV and significantly en- hanced solubility of ARV in various aqueous and bio-relevant media. (nadph-oxidasesignaling.com)
  • Loss of K8 in Caco-2 leads to decreased levels of MMP and CL, increased mobility of mitochondria and fragmented mitochondrial network as well to an aggregation of Cav1 protein, all which may affect to the energy metabolism in Caco-2 cells. (utupub.fi)
  • To study the problem of the energy metabolism control in cancer cells, Moreno-Sanchez and Westerhoff´s groups have applied the Metabolic Control Analysis (MCA) with the conclusion that the role of OXPHOS in tumor cells should be re-evaluated and experimentally determined for each particular type of tumor cell [1]. (mitophysiology.org)
  • MCA helps to understand how the control is shared between the enzymes and transporters of the pathway and to identify the steps that could be modified to achieve a successful alteration of flux or metabolite concentration in pathways, and to inhibit cancer cells energy metabolism in selective manner. (mitophysiology.org)
  • Furthermore, studies have shown HIF-mediated Sema4D induction in the generation of the pro-angiogenic phenotype in oral squamous cell carcinoma (OSCC) and have determined its biological significance for tumor growth and vascularity ( 10 ). (jcancer.org)
  • Nutrition and metabolism in hepatocellular carcinoma. (boysandgirlsclubofcypress.com)
  • They found that B. adolescentis IM38 could inhibit nuclear factor-kappa B (NF-KB) activation in Caco-2 cells and peritoneal macrophages. (medicine.news)
  • In this study, we aim at determining the expression levels of RPS3 in a colon cancer cell line Caco-2 compared to a normal colon mucosa cell line NCM-460 and study the effects of targeting this protein by siRNA on cellular behavior. (edu.lb)
  • Up-regulation of the COX-2 and 5-LOX pathways was found in the tumor tissue in comparison with normal colon mucosa. (unifi.it)
  • These cells produce SECRETIN and are found in the MUCOSA of upper SMALL INTESTINE and PYLORIC ANTRUM in mammals. (bvsalud.org)
  • Intestinal L cells are located in the MUCOSA of distal ILEUM and COLON. (bvsalud.org)
  • In previous studies K8 knockout (K8-/-) in Caco-2 cells leaded to diminished mitochondrial respiration and decreased levels mitochondrial and caveolar calcium (Ca2+) levels. (utupub.fi)
  • The control of mitochondrial respiration was distributed across several mitochondrial processes: FCC for complex II (CII) was found to be high for CRC and healthy colon tissue, as well as in the case of Caco-2 cell culture (where the respiratory chain is controlled also by CIV) (Fig. 1). (mitophysiology.org)
  • The method of MCA has been applied to human CRC skinned fibers in comparison with healthy tissue of same type and Caco-2 tumor cell line. (mitophysiology.org)
  • Expression of M46 in KrasG12D Bac mice promoted the infiltration of myeloid-derived suppressor cells and macrophages, inhibited the infiltration of T cells, and accelerated the progression of PanINs to invasive and metastatic PDAC, while HPK1 attenuated mutant Kras-driven PanIN progression. (bvsalud.org)
  • The peptide hydrolysates of both strains exhibited anti-inflammatory action through the modulation of pro-inflammatory mediators in LPS-stimulated THP-1 macrophages (COX-2, Pro-IL-1β, IL-1β, and IL-8) and LPS-stimulated HT-29 cells (IL-8). (calbinotox.fr)
  • and COX-2 in LPS-stimulated THP-1 macrophages. (calbinotox.fr)
  • Here, we discuss some aspects of metabolism and absorption of phenolic compounds. (docksci.com)
  • ABSORPTION AND METABOLISM OF POLYPHENOLIC COMPOUNDS The concentrations of polyphenolic compounds in the bloodstream depend on their modifications during metabolism and absorption form the gastrointestinal tract. (docksci.com)
  • Intestinal barrier function is the ability of the epithelial cell layer of the intestinal wall to selectively pass substances across. (utupub.fi)
  • The role of K8 in barrier function in Caco-2 cells was studied with a Cellzscope+ device. (utupub.fi)
  • The apical junctional complex comprises tight junctions, adhesion junctions, and desmosomes, which seal adjacent cells, regulate paracellular transportation, and maintain intestinal barrier function, acting as a dynamic mechanical barrier [ 5 ]. (biomedcentral.com)
  • The present invention provides a simple and robust human liver cell-based system in which persistent hepatitis C infection, persistent hepatitis B infection or ethanol exposure induces a clinical Prognostic Liver Signature (PLS) high-risk gene signature. (sumobrain.com)
  • Transcriptome analyses revealed that Caco-2 BBE cells respond to stimulation with IL-8 supporting the hypothesis that IL-8 induces G protein-coupled receptor signalling. (biomedcentral.com)
  • Photodynamic therapy with the novel photosensitizer chlorophyllin f induces apoptosis and autophagy in human bladder cancer cells. (boysandgirlsclubofcypress.com)
  • In addition, these two binding sites play different roles in the regulation of Sema4D expression in HUVEC and Caco-2 cells. (jcancer.org)
  • The ethyl acetate extract of orange juice and these methoxyflavones also increased steady-state [ 3 H]vinblastine uptake by LLC-GA5-COL300 cells (a cell line transfected with human MDR1 cDNA). (aspetjournals.org)
  • We therefore undertook a preliminary screening study of the three IFBTS seed oils in vitro using human colon adenocarcinoma cells (Caco-2), a commonly used gastrointestinal cell line, and human embryonic kidney (HEK-293), an epithelial immortalised cell line. (iiarjournals.org)
  • The HEK-293 cell line has been used extensively ( 16 - 17 ) as a laboratory work-horse in several cell culture studies. (iiarjournals.org)
  • In this work, we analyze expression patterns of DNMT3A in patients with IBD, perform loss of function experiments using Caco-2 cell line and generate a conditional Dnmt3a ΔIEC mouse line. (nature.com)
  • 8. Antony J. Debroy S. Manisha C. Thomas P. Jeyarani V. Choephel T. In-vitro cell line Models and Assay methods to study the Anti-diabetic Activity. (rjptonline.org)
  • CXCR1 is expressed in the human IEC line Caco-2 BBE [ 3 ], which is in agreement with our own microarray data using Caco-2 BBE cell mRNA (see below). (biomedcentral.com)
  • The human colon epithelial cancer cell line, Caco-2, is used as an in vitro model to simulate the human intestinal tissue. (themama.ai)
  • Cell viability was measured using the MTT assay. (hindawi.com)
  • Sequencing and alignment of this region in 11 cancer cell lines and 4 normal cell lines were also performed, followed by cloning, mutation and luciferase assay analysis. (jcancer.org)
  • CaCO-2 cell uptake assay and human liver microsome studies proved that ARV is a substrate of CYP3A4 but not of P-gp effluX pump. (nadph-oxidasesignaling.com)
  • Overview of metabolism and bioavailability enhancement of polyphenols. (docksci.com)
  • 2023 . Arsenic and iron bioavailability in Caco-2 cells: The influence of their co-existence and concentration. (ucas.ac.cn)
  • 2022 . Predictive capabilities of in vitro colon bioaccessibility for estimating in vivo relative bioavailability of arsenic from contaminated soils: arsenic speciation and gut microbiota considerations. (ucas.ac.cn)
  • By administering a selective ATF6 activator, the impairment of intestinal barrier integrity and dysregulation of CHOP/β-catenin/p-p120-catenin pathway was reversed in Pcdh20 -ablated mice with colitis and PCDH20 -deficient colonic cell lines. (biomedcentral.com)
  • Destruction of intestinal barrier integrity is one of the first phases of CD pathogenesis [ 2 ], while its repair is the final, yet most important, phase of mucosal healing in CD treatment [ 3 ]. (biomedcentral.com)
  • CT26 SC tumors: Gating strategy T cells and NK cells. (td2inc.com)
  • Hypoxia is the most common characteristic in the microenvironment of solid tumors because tumor progression and metastasis depend on the ability of cancer cells to initiate angiogenesis and ensure the delivery of oxygen and nutrients ( 11 , 12 ). (jcancer.org)
  • Accordingly, the expression of human HMGCS2 is down-regulated in 90% of Myc-dependent colon and rectum tumors. (aacrjournals.org)
  • Conjugation of glucose with metabolic agents to selectively target cancer cells was inspired by the widespread use of radiolabeled glucose analog to visualize tumors and their metastases. (oncotarget.com)
  • HMGCS2 , the gene that regulates ketone body production, is expressed in liver and several extrahepatic tissues, such as the colon. (aacrjournals.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Secretin also has been found in cells in the BRAIN and other tissues. (bvsalud.org)
  • In the Caco-2 BBE model of polarized villus-like epithelium, apical stimulation with TLR2 and TLR5 ligands resulted in the apical secretion of IL-8. (biomedcentral.com)
  • Stimulation of polarized Caco-2 BBE cells with Pam2CSK4 and Pam3CSK4, agonists for TLR2/6 and TLR2/1 from the apical or basolateral sides induced secretion of IL-8 predominantly into the apical compartment regardless of the location of the stimulus, although basolateral stimulation induced smaller amounts of IL-8 compared to apical stimulation. (biomedcentral.com)
  • Furthermore, Il-9 mRNA levels were induced in Pur-α-deficient T cells. (bvsalud.org)
  • Reduction of Il-9 mRNA levels in T cells and allergic responses of T-Dusp8-cKO mice was reversed by Pur-α knockout. (bvsalud.org)
  • however, the in vivo role of DUSP8 in T cells remains unclear. (bvsalud.org)
  • Suppressing mTOR activity, as well as levels and activity of the downstream translation regulators, eIF4E and eIF4A, delayed breast cancer progression, onset of associated pulmonary metastasis in vivo and breast cancer cell invasion and migration in vitro. (edu.lb)
  • Several functional components of the epithelium contribute to this unique role, among them the secretion of a mucus layer and life-long regeneration of the different epithelial cell types from a stem cell compartment, allowing for quick repair of barrier defects 2 . (nature.com)
  • Accordingly, immunohistochemistry with specific antibodies shows that HMGCS2 is expressed mainly in differentiated cells of human colonic epithelium. (aacrjournals.org)
  • All three tree seed oils suppressed both Caco-2 and HEK-293 cell growth in a dose-dependent manner. (iiarjournals.org)
  • However, the concurrent knockdown of RPS3 and p53 showed no change in LDH level in Caco-2 cells suggesting an interesting interplay among the three proteins. (edu.lb)
  • Probiotics and Antimicrobial Proteins, 15 (2), pp. 387-399. (calbinotox.fr)
  • Most of the identified peptides originated from bacterial cell surface proteins. (calbinotox.fr)
  • In order to explore the underlying mechanisms, we performed tests to assess cell migration and motility, apoptosis, expression of glucose transporter 1 and proapoptotic proteins. (oncotarget.com)
  • Fatty acid‐binding proteins (FABPs) are in charge of binding and storing AGAP1 hydrophobic ligands such as for example long‐chain essential fatty acids as well as for transporting these ligands to the correct compartments inside the cell. (mdm2-inhibitors.com)
  • It might be interesting to assess if the regulatory systems root the upregulation of gene appearance and the features of FABP5 proteins in tumor cells are mediated with a common signaling pathway. (mdm2-inhibitors.com)
  • Specifically although FABP5 may be the most upregulated proteins in the FABP family members comprising ten isoforms 18 the molecular features of FABP5 in CRC cells stay badly characterized. (mdm2-inhibitors.com)
  • Caco-2 cells are known to form tight junctions ( 13 - 14 ), have structural properties similar to those of ileal enterocytes ( 15 ) and are hence useful models as they mimic the gastrointestinal tract. (iiarjournals.org)
  • It is of interest to explore the inhibitory effect of adenine on the growth of colorectal cancer cells and to determine the underlying mechanism. (hindawi.com)
  • We explored the possible mechanisms underlying the anticancer activity of adenine in colorectal cancer cells. (hindawi.com)
  • We aimed at evaluating whether combined blocking of the COX-2 and 5-LOX pathways might have additive antitumor effects in colorectal cancer. (unifi.it)
  • The expression/activity of COX-2 and 5-LOX were assessed in 24 human colorectal cancer specimens. (unifi.it)
  • 2023 . Role of microbial iron reduction in arsenic metabolism from soil particle size fractions in simulated human gastrointestinal tract. (ucas.ac.cn)
  • Cells found throughout the lining of the GASTROINTESTINAL TRACT that contain and secrete regulatory PEPTIDE HORMONES and/or BIOGENIC AMINES. (bvsalud.org)
  • A balanced gut flora is essential for the regulation of human metabolism. (medicine.news)
  • This is through the regulation of Proteobacteria to Bacteroidetes ratio and NF-KB activation in the colon. (medicine.news)
  • Peptide hydrolysate significantly decreased secretion of pro-inflammatory cytokine IL-8 in lipopolysaccharide (LPS)-stimulated human colon epithelial HT-29 cells. (calbinotox.fr)
  • However, it is not known if CXCR1 is expressed on the apical or basolateral poles in polarized Caco-2 BBE cells or human intestinal tissue or whether the direction of TLR signalling influences the direction of IL-8 secretion. (biomedcentral.com)
  • Here we investigated IL-8 secretion in response to TLR signalling using intestinal Caco-2 BBE cells grown in the Transwell system. (biomedcentral.com)
  • Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport. (gu.se)
  • In PAE cells, soluble Sema4D promoted cell migration and developed a "tubulogenic" phenotype ( 4 ). (jcancer.org)
  • In neutrophils, IL-8 binding to CXCR1 triggers G protein-coupled signalling and formation of second messengers that mediate cellular migration, exocytosis of effectors and a respiratory burst to facilitate oxygen-dependent killing of phagocytosed microorganisms [ 2 ]. (biomedcentral.com)
  • Most importantly, ARV showed promising cytotoXicity, anti-migration and apoptotic activity against vemurafenib-resistant melanoma cells. (nadph-oxidasesignaling.com)
  • No significant effect was shown in normal colon NCM-460 cells. (edu.lb)
  • Human normal colon fibroblasts (CCD‐18Co) were cultured in Eagle's minimum essential medium (Sigma‐Aldrich). (mdm2-inhibitors.com)
  • Cyclooxygenase (COX)-2 and 5-lipoxygenase (5-LOX) are key enzymes involved in arachidonic acid metabolism. (unifi.it)
  • Besides chronic diseases, ACE2 is the host receptor for SARS-CoV or SARS-CoV-2 virus, mediating the first step of virus infection. (bvsalud.org)
  • IL-8 can signal through two receptors, CXCR1 and CXCR2 both of which are members of the G-protein-coupled receptor (GPCR) family [ 2 ]. (biomedcentral.com)
  • Additionally, we determined the localization of the CXCR1 receptor in Caco-2 BBE cells and human intestinal tissue samples and investigated the role of IL-8 autocrine signalling using transcriptomics. (biomedcentral.com)
  • The data showed that adenine activated AMP-activated protein kinase (AMPK) signaling contributing to autophagic cell death through mTOR in both colon cancer cell lines. (hindawi.com)
  • Recently, adenine has been explored for its anticancer property in several types of cancer cell lines. (hindawi.com)
  • There were three nucleotide variants (T471C/A600G/C862T) frequently detected in cancer cell lines. (jcancer.org)
  • Two novel compounds labelled as thioglycoside A and B were designed and evaluated on breast and colon cancer cell lines. (oncotarget.com)
  • The anti-inflammatory activity of peptide hydrolysate was investigated under inflammatory conditions in two cell models. (calbinotox.fr)
  • Anticancer activity of adenine in colon cancer cells is attributable to the activation of apoptotic signaling and in turn the AMPK/mTOR pathway. (hindawi.com)
  • Inhibition of either COX-2 or 5-LOX alone resulted in activation of the other pathway in colon cancer cells. (unifi.it)
  • In comparison to the conventional Caco-2 Transwell model, Daxiang's intestinal model utilizes human intestinal organoids and incorporates the necessary fluid shear force found in the tissue microenvironment, resulting in a model that more closely mimics the normal physiological state of humans at the cellular level. (daxiangbio.com)
  • The present study focuses on the physiological functions of FABP5 in CRC cells and assesses the effects of FABP5 expression on CRC cell progression. (mdm2-inhibitors.com)
  • In this study the involvement of K8 on mitochondrial function was further examined in K8-/- Caco-2 cells by microplate reader assays to determine the levels of mitochondrial membrane potential (MMP) and cardiolipin (CL), which are both needed for normal mitochondrial function. (utupub.fi)
  • K. africana oil significantly suppressed Caco-2 cell growth compared to HEK-293 cell growth at all oil concentrations, whereas M. zeyheri and X. caffra seed oils significantly suppressed HEK-293 and Caco-2 cell growth, only at a concentration of 80 mg/l. (iiarjournals.org)
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 10x lens). (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 40x lens). (ptglab.com)
  • Remarkably, DUSP8 protein levels and the DUSP8-Pur-α interaction were indeed increased in the cytoplasm of T cells from people with asthma and patients with atopic dermatitis. (bvsalud.org)
  • Moreover, soluble ACE2 protein and exosomal ACE2 protein facilitate SARS-CoV-2 infection into host cells. (bvsalud.org)
  • On the traditional Transwell, the cells only maintain a dense cobblestone structure. (daxiangbio.com)
  • Based on these findings, we propose that ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2 . (aacrjournals.org)
  • Further research on the systems regulating gene appearance in tumor cells are actually in progress inside our laboratory. (mdm2-inhibitors.com)
  • Knockdown of FABP5 gene by siRNA was conducted as follows: cells were transfected with 20 nm unfavorable control siRNA or FABP5 siRNA (IDT HSC.RNAI.N001444.12.1 and HSC.RNAI.N001444.12.7) using Lipofectamine RNAiMAX (Thermo Scientific) according to manufacturer instructions. (mdm2-inhibitors.com)