Collagen functional cartilage on scaffolds tissue engineering. Medical search. Frequent questions

This creates issues in regeneration of the tissue. (wikipedia.org)
Cells: Chondrocytes are an obvious choice to use in the regeneration of cartilage due to their ability to secrete collagen and other ECM components necessary for the functional properties of cartilage. (wikipedia.org)
Mesenchymal stem cells can also be used to create chondrocytes and make cartilage regeneration possible. (wikipedia.org)
Electrospun Scaffolds in Tendons Regeneration: a review. (chop.edu)
Tissue engineering is a multidisciplinary field that applies the principles of engineering and life-sciences for regeneration of damaged tissues. (wjgnet.com)
The report discusses various biomaterials being developed for medical applications and describes how biomaterials have been proven to be useful in medical implants, tissue healing and regeneration, as well as in molecular probes, biosensors, and advanced drug delivery systems. (biomatdb.eu)
Thus, IGF has great potential to promote the regeneration of articular cartilage after injur y[ 5 ]. (biomedcentral.com)
The nanofiber scaffold produced by the invention can be used for specific tissue engineering applications, including bone, cartilage, blood vessel and skin tissue regeneration. (nanofiberlabs.com)
In actual preparation, electrospun PLLA scaffold will be prepared in combination with surface modification to guide cells to differentiate into bone lineage and achieve the best bone regeneration performance. (nanofiberlabs.com)
In addition, the appropriate size of the scaffold hole also improves the functional characteristics of cartilage, thus providing some different solutions for the regeneration and repair of cartilage tissue. (nanofiberlabs.com)
In addition, the evaluation of genes and proteins showed that the wound healing was active, which again confirmed that the double-layer scaffold could provide a suitable microenvironment for stimulating skin regeneration. (nanofiberlabs.com)
Although mesenchymal stem cells (MSCs) have been shown as a novel approach in tissue regeneration, the therapeutic potential of MSCs mediated by the interaction between MSC-derived paracrine mediators and Mφs remains elusive. (frontiersin.org)
3. Browe D.C., Burdis R., Diaz Payno P., Freeman F.E., Nulty J.M., Buckley C.T., Brama P.A.J. and Kelly, D.J. Promoting endogenous articular cartilage regeneration using extracellular matrix scaffolds. (buckleylab.eu)
16. Buckley C.T., Hoyland J.A., Fujii K., Pandit A., Iatridis J.C. and Grad S. Critical aspects and challenges for intervertebral disc repair and regeneration - Harnessing advances in tissue engineering. (buckleylab.eu)
17. Gansau, J. and Buckley C.T. Incorporation of collagen and hyaluronic acid to enhance the bioactivity of fibrin-based hydrogels for nucleus pulposus regeneration. (buckleylab.eu)
However, MSCs have gained considerable attention for tissue regeneration owing to their differentiation ability with immunomodulatory effects. (techscience.com)
Tissue-regeneration engineering has become an important method for articular cartilage repair. (finfactbuddy.com)
She was awarded with a postdoc fellow from the Ministry of Science at the Technical University of Catalonia in Biomaterials for bone tissue regeneration and since 2010 has been professor at this university. (ibecbarcelona.eu)
In this review, biomaterial scaffolds and cell-based therapies for IVD regeneration are briefly discussed. (bjbms.org)
Her research group is particularly interested in developing biomaterials and cell-based therapeutics to improve regeneration of various musculoskeletal tissues including bone, cartilage, tendon, blood vessels etc. (stanford.edu)
Subsequent regenerative dental procedures include the development of guided tissue or bone regeneration (GTR, GBR) procedures and distraction osteogenesis 2 the application of platelet rich plasma (PRP) for bone augmentation 17 . (bvsalud.org)

The extracellular matrix (ECM) of collagen is what gives it its high strength. (wikipedia.org)
Collagen: Collagen is a structural protein present in the extracellular matrix of cartilage. (wikipedia.org)
Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering. (chop.edu)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
Articular cartilage is mainly composed of chondrocytes and dense extracellular matrix (ECM) without blood vessels or innervation. (biomedcentral.com)
The significantly different biomechanical properties of the artificial scaffolds highlight the necessity of cellular ingrowth and formation of extracellular matrix to gain viscoelastic properties. (biomedcentral.com)
8 nm).Ppy NPs effectively augment the conductivity, surface roughness, andthickness of BC composites despite reducing scaffolds' transparency.BC-Ppy composites were flexible (up to 10 mM Ppy), maintained theirintricate 3D extracellular matrix-like mesh structure in all Ppy concentrationstested, and displayed electrical conductivities in the range of nativecardiac tissue. (ibecbarcelona.eu)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)

Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)
ACS Biomaterials Science & Engineering. (chop.edu)
Chen F, Liu X. Advancing biomaterials of human origin for tissue engineering. (scielo.br)
Current research in tissue engineering is geared towards elucidating the appropriate compositional elements (biomaterials, biomolecules and cell sources) as well as methods of assembly. (biomedcentral.com)
Cruz Perez B, Tang J, Morris HJ, Palko JR, Pan X, Hart RT, Liu J. Scaffold download drawing made easy dragons fantasy unleash your creative beast as you conjure up biomaterials presence giving, hiringContact, and siehe coverage. (it-dresden.net)
Tissue Engineering - Part A is a journal indexed in SJR in Biochemistry and Biomaterials with an H index of 128. (countryofpapers.com)
She is the Director and PI for Stem Cells and Biomaterials Engineering Laboratory at Stanford University, jointly supported by Departments of Orthopaedic Surgery and Bioengineering. (stanford.edu)
Dr. Yang has extensive expertise in developing novel biomaterials and stem cell-based therapeutics for musculoskeletal tissue engineering, or engineering 3D in vitro cancer models for drug screening and mechanistic discovery. (stanford.edu)
We invent biomaterials and engineering tools to elucidate and modulate biology, and also use biology to inform materials and engineering design. (stanford.edu)

Chondrocytes vary in size, shape and concentration depending on their location in articular cartilage. (wikipedia.org)
Additionally, aging chondrocytes show less metabolic activity and may not produce functional proteins or not enough functional proteins to create a desired ECM. (wikipedia.org)
The first tissue-engineered, ear-shaped appendages made from bovine chondrocytes and biocompatible scaffolds by the Vacanti group were prone to deformation when xenografted onto immune-compromised mice, highlighting the lack of long-term stability [ 6 ]. (biomedcentral.com)
Strategies for IGF delivery to chondrocytes and cartilage matrix are essential for its clinical application in OA treatment. (biomedcentral.com)
When cartilage is damaged, MSCs derived from synovial fluid can partially move to the injured site and differentiate into chondrocytes to repair the defect, and IGF-1 induces chondrogenic differentiation [ 11 , 12 , 13 ]. (biomedcentral.com)
Methods: Human chondrocytes were harvested from 12 osteoarthritic knee joints and seeded into a type I collagen (col-I) hydrogel at low density (2×105 cells/ml gel). (iospress.com)
has shown promising Moxonidine Hydrochloride results for development of designed cartilage using animal chondrocytes and Moxonidine Hydrochloride stem cells (O'Connell using chondrocytes and stem cells derived from numerous animal models (Farrell functional cartilage tissue development of non-human mammalian cells encapsulated in agarose (Sampat (2012). (biongenex.com)
Regenerative Potential of Tissue-Engineered Nasal Chondrocytes in Goat Articular Cartilage Defects. (unibas.ch)
8. Carroll, S.F., Buckley, C.T., Kelly, D.J. Measuring and modelling oxygen transport and consumption in 3D hydrogels containing chondrocytes and stem cells of different tissue origins. (buckleylab.eu)

Artificial cartilage is a synthetic material made of hydrogels or polymers that aims to mimic the functional properties of natural cartilage in the human body. (wikipedia.org)
The growth of hydrogels across six applications has been significant over the last two decades: smart materials, advanced drug delivery, tissue engineering, antimicrobials, bioelectronic materials, and brain-machine interface. (biomatdb.eu)

In the tangential zone, collagen fibers are aligned parallel to the surface and become gradually randomly aligned while moving into the deep zone. (wikipedia.org)
Collagen fibers in the superficial zone are aligned parallel to the surface in order to restrict shear stresses. (wikipedia.org)
Similarly, collagen fibers are aligned perpendicular to the surface in the deep zone in order to restrict compressive forces. (wikipedia.org)
These connective tissues are made up of bundles of collagenous fibers arranged in parallel, slightly wavy or curved arrays. (asianarthroscopy.com)
Collagen fibers make 65 to 80% of the dry mass. (asianarthroscopy.com)
Microscopically the attachment point shows interdigitation of collagen fibers with transition from tendon, unmineralized fibrocartilage, mineralized fibrocartilage and bone. (asianarthroscopy.com)
PBM therapy allowed an improvement in the formation of new bone, with a more organized deposition of collagen fibers in the defect area. (scielo.br)
In bone tissue engineering, electrospinning has been widely used to prepare nanofiber scaffolds, whose structure is close to the nano collagen fibers of bone. (nanofiberlabs.com)
The papillary dermis is thinner, consisting of loose connective tissue that contains capillaries, elastic fibers, reticular fibers, and some collagen. (medscape.com)
The reticular dermis consists of a thicker layer of dense connective tissue containing larger blood vessels, closely interlaced elastic fibers, and coarse, branching collagen fibers arranged in layers parallel to the surface. (medscape.com)

Nasal chondrocyte-based engineered autologous cartilage tissue for repair of articular cartilage defects: an observational first-in-human trial. (unibas.ch)
Bone grafting is a common procedure in orthopaedic surgery and the implantation of autologous bone grafts supplying osteoinductive growth factors, osteogenic cells, and a structural scaffold, has become the gold standard for the surgical treatment of bone defects caused by trauma, tumor, infection or congenital abnormalities. (biomedcentral.com)
Because of their capacity for ex vivo proliferation and differentiation they provide a good source of osteoprogenitor cells within custom-shaped scaffolds for implantable autologous bone tissue thus allowing the generation of a large transplantable cell population from a small biopsy [ 5 - 11 ]. (biomedcentral.com)

The association of scaffolds to repair extensive bone defects can contribute to their evolution and morphophysiological recomposition. (scielo.br)
This PhD work was aimed to design, develop, and characterize gelatin-based scaffolds, for the repair of defects in the muscle-skeletal system. (unibo.it)
These conservative strategies can alleviate pain symptoms, but they cannot terminate the progression of cartilage deterioration and repair cartilage defects. (biomedcentral.com)
Focal chondral defects of the knee joint refers to localized areas of damage or loss of articular cartilage in the knee. (finfactbuddy.com)
These defects can range in size and depth, from small lesions to larger areas of cartilage damage. (finfactbuddy.com)
Focal chondral defects can be challenging to manage as articular cartilage has limited regenerative capacity. (finfactbuddy.com)
If left untreated, these defects can progress, leading to further cartilage degeneration, joint instability, and the development of osteoarthritis (OA). (finfactbuddy.com)
The treatment of focal chondral defects aims to promote cartilage repair and restoration of joint function. (finfactbuddy.com)

Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
Fig. 3 Small Aperture (125-250 μ m) In vitro cartilage differentiation of human bone marrow mesenchymal stem cells induced by β - lactamase scaffold Large specific aperture (425-600 μ m) The stents in our body better support cartilage formation. (nanofiberlabs.com)
For the quantification of cellular differentiation at the molecular level, osteogenic differentiation of MSCs is controlled by the interaction of hormones and transcription factors: runt-related transcription factor-2 (RUNX2) effectuates the expression of bone-specific genes, e.g. osterix (OSX), collagen type 1 alpha-1 (COL1A1), osteocalcin (OC), and bone sialoprotein (BSP) by binding to the promoters of these genes. (biomedcentral.com)
Enhanced expression of IL-6, IGF-1, TGF-β, and VEGF has also been observed in LC treated BMSCc+ groups, suggested the cardiac differentiation of BMSCc+, and can be utilized in tissue engineering for cardiac cell therapy. (techscience.com)
Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)

Journal of Tissue Engineering and Regenerative Medicine. (chop.edu)
Recent advances in regenerative medicine place us in a unique position to improve the quality of engineered tissue. (biomedcentral.com)
Combining biomimetic regenerative medicine strategies will allow us to improve tissue-engineered auricular cartilage with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
Due to recent advances in regenerative medicine and additive manufacturing we are entering into an age where we have the potential to replace 'like with like', by improving the quality of engineered tissue with respect to biochemical composition and functionality, as well as microstructural organization and overall shape. (biomedcentral.com)
We will use the lessons learnt from auricular cartilage tissue engineering to illustrate how combining additive manufacturing and regenerative medicine for tissue-engineering purposes can be used to create functional and durable tissue with potential to shift the paradigm in reconstructive surgery. (biomedcentral.com)
In: Encyclopedia of Tissue Engineering and Regenerative Medicine, 1. (unibas.ch)
The research in Dr. Vorp's lab focuses on the biomechanics, "mechanopathobiology," regenerative medicine, and tissue engineering of tubular tissues and organs, predominantly the vasculature. (pitt.edu)
For many years there is an urgent need in the field of regenerative medicine to develop replacements of non-functional tissues. (nature.com)
Macrophages (Mφs) are the critical regulators involved in initiation, propagation, and resolution of inflammatory response throughout the tissue regenerative process. (frontiersin.org)
Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
The low endogenous regenerative capacity of the heart,added tothe prevalence of cardiovascular diseases, triggered the advent ofcardiac tissue engineering in the last decades. (ibecbarcelona.eu)
This significantly impacted the development of tissue engineering in various medical fields, including regenerative techniques for intervertebral disc (IVD) degeneration (IDD). (bjbms.org)
Although current treatment modalities offer high levels of success for many conditions, an ideal form of therapy might consist of regenerative approaches in which diseased or necrotic pulp tissues are removed and replaced with healthy pulp tissue to revitalize teeth. (bvsalud.org)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
One novel approach to restore tooth structure is based on regenerative endodontic procedures by application of tissue engineering. (bvsalud.org)
The purpose of this article is to review the biological principles of tissue engineering and the hurdles that must be overcome to develop regenerative endodontic procedures. (bvsalud.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)

Angiogenesis is an important factor for tissue-engineered skin constructs. (hindawi.com)
Scaffold choice, immunogenicity, degradation rate, toxicity of degradation products, host inflammatory responses, fibrous tissue formation due to scaffold degradation, and mechanical mismatches with the surrounding tissue are key issues that may affect the long-term behavior of the engineered tissue constructs and directly interfere with their primary biological functions [ 17 ]. (hindawi.com)
Current tissue-engineered auricular constructs implanted into immune-competent animal models have been observed to undergo inflammation, fibrosis, foreign body reaction, calcification and degradation. (biomedcentral.com)
However engineered cartilage development within agarose was better for constructs cultured with TGFβ3. (biongenex.com)

A recent publication titled "Co-electrospun PCL/Zein membranes as scaffolds for articular cartilage engineering" by the ETH team was featured in MDPI Bioengineering. (biotrib.eu)
He was the Chair of the Bioengineering Division of the American Society of Mechanical Engineers (ASME), the first non-MD President of the International Society for Applied Cardiovascular Biology, and the Secretary of the Executive Committee of the Biomedical Engineering Society (BMES), for which he served on the Board of Directors for 10 years. (pitt.edu)

Here he also utilizes mesenchymal stem cells or their products incorporated in a biodegradable scaffold that undergoes substantial in vivo remodeling to develop a native-like blood vessel. (pitt.edu)
Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Seeded scaffolds were implanted and studied in the post-mortem murine heart in situ, and in two additional C57BL/6 mice in vivo (using single-layered and double-layered scaffolds) and imaged immediately after and at 7 days post-implantation. (westminster.ac.uk)
In vitro MRI results justify the increased seeding density, increased in vitro MRI signal, and improved MRI visibility in vivo, in the double-layered compared to the single-layered scaffolds. (westminster.ac.uk)
An ex vivo study showed that IGF-1 in fetal bovine serum was responsible for maintaining articular cartilage proteoglycan synthesis. (biomedcentral.com)
PLLA nanofiber scaffold made by phase separation and pore forming agent leaching has been proved to be the optimal scheme for cartilage repair in vivo and in vitro. (nanofiberlabs.com)
On the other hand, in vivo wound healing evaluation showed that the composite scaffold healed faster and more effectively than the collagen scaffold. (nanofiberlabs.com)
Effects of an articular cartilage lubrication with a viscosupplement in vitro and in vivo following osteochondral fractures in horses. (ucsd.edu)
Fibrin-filled scaffolds for bone tissue engineering: an in vivo study. (zju.edu.cn)

In this work, gelatin based scaffolds were prepared following three different strategies: films were prepared through the solvent casting method, electrospinning technique was applied for the preparation of porous mats, and 3D porous scaffolds were prepared through freeze-drying. (unibo.it)
Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. (zju.edu.cn)

Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
Marrow-derived stem cell motility in 3D synthetic scaffold is governed by geometry along with adhesivity and stiffness. (nature.com)
We use auricular cartilage as an exemplar to illustrate how the use of tissue-specific adult stem cells, assembly through additive manufacturing and improved understanding of postnatal tissue maturation will allow us to more accurately replicate native tissue anisotropy. (biomedcentral.com)
The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
All tissues originate from stem cells. (bvsalud.org)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

A successful method was developed to prepare gelatin nanofibrous scaffolds electrospun from acetic acid/water solution and stabilized with a non-toxic crosslinking agent, genipin, able to preserve their original morphology after exposure to water. (unibo.it)
Moreover, the co-electrospinning technique was used to prepare nanofibrous scaffolds at variable content of gelatin and polylactic acid. (unibo.it)

Sustained hypoxia or lack of nutrients leads to fibroblast dysfunction, decreased keratinocyte migration and proliferation, and tissue loss [ 6 ]. (hindawi.com)
The repair process begins with the formation of the clot, accompanied by an inflammatory process with proliferation of granulation tissue. (scielo.br)
Preliminary in vitro tests show good osteoblast response in terms of proliferation and adhesion on all the scaffolds. (unibo.it)
In a rat fracture model, IGF-1 combined with TGF-ß could stimulate chondrocyte proliferation and cartilage formation at the early stage of day 5 [ 8 ]. (biomedcentral.com)
The final evaluation experiment results showed that the collagen in the middle layer significantly improved the adhesion and proliferation of SMC, and the proliferation of endothelial cells increased significantly with culture, indicating the non cytotoxicity of the construct. (nanofiberlabs.com)
In dermal fibroblasts culture in vitro, the mixed scaffold induced higher cell seeding efficiency and improved fibroblast adhesion and proliferation compared with collagen sponge. (nanofiberlabs.com)
The final results showed that the cell proliferation in the double-layer scaffold was higher than that in the single-layer CP nano pad and PLLA microdisk. (nanofiberlabs.com)

There are three structural zones in articular cartilage including a superficial tangential zone, a middle transitional zone and a deep zone. (wikipedia.org)
Each of the following algorithms comprises one of the three stimulation groups, which were chosen to produce characteristics similar to the superficial tangential zone of articular cartilage: application of compression and tension without offset, application of compression and tension with offset, and tension alone. (lievers.net)
Western blot results showed the group with compression and tension without offset had similar aggrecan content to native articular cartilage results, as well as the other stimulation groups, indicating stimulation aids in producing aggrecan content similar to the superficial tangential zone. (lievers.net)

Maroudas A. Physicochemical properties of cartilage in the light of ion exchange theory. (lievers.net)

Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
The future trend of grafts for ACL reconstruction might not be same as we see today because of on going research like tissue engineering. (asianarthroscopy.com)
One way is to carry out the transplantation, but the success of this strategy is complicated by immune reactions to allogeneic or xenogeneic grafts or deficiency of donor tissue. (nature.com)

There are three types of cartilage in the human body: fibrocartilage, hyaline cartilage and elastic cartilage. (wikipedia.org)
Fibrocartilage is most often found in the intervertebral discs, elastic cartilage is found in the external ear and hyaline cartilage is found on many joint surfaces in the body. (wikipedia.org)
Replacement of hyaline cartilage (articular cartilage) is the most common application of synthetic cartilage. (wikipedia.org)
However, clinical efficacy of these treatments is not conclusive, as these treatments do not consistently regenerate hyaline cartilage. (biomedcentral.com)

Dynamic Compressive Loading Improves Cartilage Repair in an in vitro Model of Microfracture: Comparison of Two Mechanical Loading Regimens on Simulated Microfracture Based on Fibrin Gel Scaffolds Encapsulating Connective Tissue Progenitor Cells. (chop.edu)
Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells. (unibas.ch)
However, it can be challenging to differentiate when they are connected with wounded tissue, opposed to when they instruct tissue-specific progenitor cells responsible for the redevelopment of damaged tissue. (techscience.com)

Tissue engineering principles are used in order to create a non-degradable and biocompatible material that can replace cartilage. (wikipedia.org)
The scaffold material should be biocompatible, i.e., non-toxic and non-immunogenic, biodegradable, and at the same time, ecological and easy to manufacture, thus economically viable. (nature.com)

Gioffré, Michela (2013) Biodegradable systems for the development of functional materials , [Dissertation thesis], Alma Mater Studiorum Università di Bologna. (unibo.it)
PLLA is one of the first synthetic polymer materials recognized as suitable for tissue engineering due to its non-toxic, non irritating, biodegradable absorption and high strength. (nanofiberlabs.com)

Type 1 collagen is the one which is abundant in both with some type 3 collagen. (asianarthroscopy.com)

Finally, 3D porous gelatin scaffolds, enriched with calcium phosphate, were prepared with the freeze-drying method. (unibo.it)
In most cases, PLLA, collagen and gelatin are often combined to produce nanofiber scaffolds, which show physical and biological characteristics matching with skin substitutes. (nanofiberlabs.com)
In this study, porous scaffold materials based on polyvinyl alcohol (PVA) and gelatin (Gel) were successfully fabricated and characterized. (mdpi.com)

Programmed PDGF-BB and BMP-2 Delivery from a Hybrid, Calcium Phosphate/ Alginate Scaffold. (chop.edu)

Articular cartilage repair is a critical issue in osteoarthritis (OA) treatment. (biomedcentral.com)
Osteoarthritis (OA) is characterized by the progressive destruction of articular cartilage, which seriously restricts sports ability and impacts quality of life. (biomedcentral.com)
Due to the high incidence of injury and osteoarthritis and the limited nature of articular cartilage self-repair, alternate means are necessary to replace the damaged tissue and regain an articulating surface that not only stops the progression of disease but functions the same as a healthy articular surface. (lievers.net)
Further exploration with both compression and tension without offset stimulation in longer culturing can enhance these aspects and lead to prevention of further cartilage degradation in patients with early signs of osteoarthritis or damaged articulating surface. (lievers.net)
Loss of meniscal tissue is correlated with early osteoarthritis but few data exist regarding detailed biomechanical properties (e.g. viscoelastic behavior) of menisci in different species commonly used as animal models. (biomedcentral.com)
It is obvious that loss of meniscus tissue leads to focal overload of articular cartilage and finally to a higher incidence of osteoarthritis [ 1 , 3 ]. (biomedcentral.com)
This intervention may prevent chronic degeneration of cartilage tissue, an irreversible degenerative process that eventually develops into osteoarthritis (OA). (finfactbuddy.com)
In osteoarthritis, the cartilage in the joints breaks down. (medlineplus.gov)

Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering[J]. Journal of Zhejiang University Science B, 2010, 11(7): 524-530. (zju.edu.cn)
To create a scaffold that is suitable for the construction of tissue-engineered skin , a novel asymmetric porous scaffold with different pore sizes on either side was prepared by combining a collagen - chitosan porous membrane with fibrin glue . (zju.edu.cn)
Epidermal cells could be seen growing easily and achieved confluence on the fibrin glue on the upper surface of the scaffold . (zju.edu.cn)

It is obtained from collagen via thermal denaturation or chemical-physical degradation. (unibo.it)

However, these methods have some drawbacks because the ECM substitutes contained exogenous materials such as bovine collagen, dead human allogenic dermis, or synthetic polymers [ 16 ]. (hindawi.com)

Actifit is a polyurethane polymer scaffold and good biocompatibility and cellular ingrowth have been reported leading to an improvement of the frictional properties as reported by Galley et al. (biomedcentral.com)
Biocompatibility and introduction to biology": Biomedical Engineering, Politecnico di Milano, sede distaccata presso San Raffaele, Segrate Mi, Italy) from 1994-1997. (unitn.it)

Tissue engineering principles include the use of cells, growth factors, and synthetic scaffolds in order to do this. (wikipedia.org)
Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
Thus, chondrocyte metabolism in the adjacent cartilage is relatively low, and these cells cannot easily migrate to the damaged site [ 2 ]. (biomedcentral.com)
These scaffolds were then used to detect the response of mouse bone marrow stromal cells after implantation of the scaffold. (nanofiberlabs.com)
These nanofibers were made into three-layer tubular scaffolds by continuous electrospinning, and cultured endothelial cells and smooth muscle cells for biological activity evaluation. (nanofiberlabs.com)
The same donor cells were expanded for 3D cultures in pellets and agarose scaffolds. (biongenex.com)
Fat-Derived Stromal Vascular Fraction Cells Enhance the Bone-Forming Capacity of Devitalized Engineered Hypertrophic Cartilage Matrix. (unibas.ch)
We hope to achieve this by creating a tissue replacement product through tissue engineering using an abundant cell source of mesenchymal stems cells seeded in Collagen Type I scaffolds, and treated not only with chondrogenic growth media in culture, but three different stimulation algorithms to improve structural and functional properties. (lievers.net)
Cells were expanded, seeded on collagen type I scaffolds and grown statically for 1 week. (lievers.net)
Therefore, many laboratories try with great effort to develop resorbable tissue scaffolds that could support the patient´s cells. (nature.com)
ECM provides mechanical support for cells and also determines the shape of tissue 9 . (nature.com)
The aim of this study is to evaluate the feasibility, safety, and efficacy of cell therapy based on mesenchymal stromal cells derived from adipose tissue intramuscular administration to patients with type 2 diabetes mellitus with critical limb ischemia and without possibility of revascularization. (unav.edu)
Cells Tissues Organs , 179 (4):158-169. (zju.edu.cn)
Recently, tissue engineering techniques emerged as a possible approach to treat IDD, by replacing a damaged IVD with scaffolds and appropriate cells. (bjbms.org)

The bone tendon interface is composed of a tissue called enthesis which is a transitional zone transmitting the stress from bone to tendon and vice versa. (asianarthroscopy.com)

The myocardial nicheplays a critical role in directing the function and fate of cardiomyocytes;therefore, engineering a biomimetic scaffold holds excellent promise.We produced an electroconductive cardiac patch of bacterial nanocellulose(BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the naturalmyocardial microenvironment. (ibecbarcelona.eu)

The constructed composite skin substitute had a histological structure similar to that of normal skin tissue after three weeks of culture. (zju.edu.cn)
[ 1 ] In addition to providing a physical barrier to pathogenic organisms, skin functions as an active immune organ with distinctive antigenic properties that play a significant role with particular regard to composite tissue allotransplantation. (medscape.com)

A research team has developed a new type of double-layer scaffold, which is composed of chitosan/PCL nanofiber pad on the surface and PLLA microporous disk on the bottom. (nanofiberlabs.com)

In all these areas, the interrelationships of rheological properties of the systems or materials investigated and their structural and functional aspects are stressed. (iospress.com)
The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. (iospress.com)

Biologically and mechanically driven design of an RGD-mimetic macroporous foam for adipose tissue engineering applications. (unibas.ch)

Overall, this research highlights the potential of co-electrospun PCL/Zein membranes as promising scaffolds for articular cartilage engineering, showcasing their improved protein adsorption properties and reduced surface roughness. (biotrib.eu)
Ferguson, S.J. Co-Electrospun Poly(ε-Caprolactone)/Zein Articular Cartilage Scaffolds. (biotrib.eu)
The information acquired on the effect of crosslinking in different conditions was utilized to optimize the preparation procedure of electrospun and freeze-dried scaffolds. (unibo.it)

Human MSCs were isolated and cultivated onto the surface of a 3 × 3 mm porcine collagen I/III carrier. (biomedcentral.com)

Nature Biomedical Engineering. (chop.edu)
2013) Elected 2013-2014 Chair of the Biomedical Engineering Division, ASME. (pitt.edu)
2009) Distinguished Lecturer, Columbia University, Department of Biomedical Engineering. (pitt.edu)
2008) Fellow, Biomedical Engineering Society (BMES). (pitt.edu)
Center for Biomedical Engineering. (acs.org)

Insulin-like growth factor-1 (IGF-1), a member of a family of growth factors that are structurally closely related to pro-insulin, has shown profound effects on chondrocyte biological behavior and fundamentally regulates cartilage matrix metabolism during cartilage repair. (biomedcentral.com)
Davisson T, Kunig S, Chen A, Sah R, Ratcliffe A. Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage. (lievers.net)

The biphasic poroviscoelastic behavior of articular cartilage: role of the surface zone in governing the compressive behavior. (lievers.net)

First, cartilage is an avascular structure in the body and therefore does not repair itself. (wikipedia.org)
The insulin-like growth factor (IGF) signaling pathway has been implicated in articular cartilage repair. (biomedcentral.com)
Optimization of IGF delivery systems will facilitate treatment application in cartilage repair and improve OA treatment efficacy. (biomedcentral.com)
Once a cartilage defect occurs, self-repair is not easy [ 3 ]. (biomedcentral.com)
Growth factors and their signaling pathways have recently attracted much attention in cartilage repair for OA treatment. (biomedcentral.com)
Learn, simplify and implement: developmental re-engineering strategies for cartilage repair. (unibas.ch)
Although the mechanical properties were inferior to native articular cartilage, they were within range of repair tissue. (lievers.net)
Poole AR, Kojima T, Yasuda T, Mwale F, Kobayashi M, Laverty S. Composition and structure of articular cartilage: a template for tissue repair. (lievers.net)
Articular cartilage repair: basic science and clinical progress. (lievers.net)
This makes cartilage repair difficult. (finfactbuddy.com)

Scaffold Composition Determines the Angiogenic Outcome of Cell-Based Vascular Endothelial Growth Factor Expression by Modulating Its Microenvironmental Distribution. (unibas.ch)
He is also designing a small diameter tissue engineered vascular graft to treat cardiovascular diseases. (pitt.edu)

Dr. Vorp has been elected Fellow of a number of prestigious organizations, including the American Heart Association, the American Institute for Medical & Biological Engineers, ASME and BMES. (pitt.edu)
2005) Fellow, American Institute for Medical and Biological Engineering (AIMBE). (pitt.edu)
Langer and Vacanti 19 defined tissue engineering as an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function. (bvsalud.org)

Preliminary in vitro tests indicated that the scaffolds are suitable for cartilage tissue engineering, and that their potential applications can be extended to cartilage-bone interface tissue engineering. (unibo.it)

Especially, studies about the biomechanical properties of theses polyurethane scaffolds at time of implantation are missing. (biomedcentral.com)

Skin tissue substitutes must readily adhere, have good physical and mechanical properties, and be nonantigenic [ 2 ]. (hindawi.com)
Polymer characterization confirmed the chemical structure and composition of the synthesized scaffolds, while thermal, wettability, and mechanical properties were also investigated and compared in neat and porous counterparts. (westminster.ac.uk)

However, when articular cartilage degeneration sets in, the structure and composition of proteoglycans break down, leading to a range of issues. (finfactbuddy.com)

download drawing made easy dragons fantasy unleash your creative beast as you conjure up dragons fairies gender as a ren ground for the zusammenzieht of notable columnar defendants forfeited on a Current human canine scaffold bonus. (it-dresden.net)
These results with agarose and pellet culture studies set the stage for the development of conditions appropriate for culturing 3D functional designed cartilage for eventual use in human therapies. (biongenex.com)
The purpose of the current study was to biomechanically characterize bovine, ovine, and porcine menisci (each n = 6, midpart of the medial meniscus) and compare their properties to that of normal and degenerated human menisci (n = 6) and two commercially available artificial scaffolds (each n = 3). (biomedcentral.com)
For future research the use of ovine meniscus would be desirable showing the highest biomechanical similarities to human meniscus tissue. (biomedcentral.com)
tissue-engineered skin was fabricated using this asymmetric scaffold , fibroblasts, and a human keratinocyte line (HaCaT). (zju.edu.cn)
Our lab's mission is to develop therapies for regenerating human tissues lost due to diseases or aging, and to build tissue engineered 3D models for understanding disease progression and informing drug discovery. (stanford.edu)
Chondroitin sulfate is a chemical found in human and animal cartilage. (medlineplus.gov)

Histological evaluations (bright-field, cytoplasmic (Atto647) and nuclear (DAPI) stains) performed in conjunction with confocal microscopy imaging attest to CPC binding within the scaffold, subsequent release and migration to the neighboring myocardium, and to increased retention in the murine myocardium in the case of the double-layered scaffold. (westminster.ac.uk)

Anatomy60

Organisms6

Diseases8

Chemicals and Drugs78

Analytical, Diagnostic and Therapeutic Techniques and Equipment50

Phenomena and Processes36

Disciplines and Occupations9

Technology, Industry, Agriculture31

Information Science4

Regeneration21

Extracellular matrix8

Biomaterials9

Chondrocytes9

Hydrogels2

Fibers10

Autologous3

Defects8

Differentiation5

Regenerative17

Constructs4

Bioengineering2

Mesenchymal Stem3

VIVO7

Porous scaffolds2

Stem7

Nanofibrous Scaffolds2

Proliferation7

Superficial tangential zone3

Properties of cartilage1

Grafts3

Hyaline cartilage4

Progenitor Cells3

Biocompatible2

Biodegradable2

Type 3 collag1

Gelatin3

Calcium Phosphate1

Osteoarthritis8

Fibrin3

Degradation1

Bovine collagen1

Biocompatibility2

Cells14

Tendon1

Biomimetic1

Composite2

Chitosan1

Structural2

Adipose tissue1

Electrospun3

MSCs1

Biomedical Engineering5

Matrix metabolism2

Behavior1

Repair10

Vascular2

Biological3

Suitable1

Implantation1

Mechanical properties2

Composition1

Human7

Histological1