• genomic
  • I would suspect that you will see a lot of large molecular weight material that would correspond to the genomic DNA, again it would depend on what kind of protocol you were using to isolate the DNA in the first place, but it might give you some indication of how pure it is. (protocol-online.org)
  • vitro
  • Soon after, the discovery and purification of REases that recognized and cut at specific sites (Type II REases) allowed scientists to perform precise manipulations of DNA in vitro , such as the cloning of exogenous genes and creation of efficient cloning vectors. (neb.com)
  • The fundamental difference between the two methods is that molecular cloning involves replication of the DNA in a living microorganism, while PCR replicates DNA in an in vitro solution, free of living cells. (wikipedia.org)
  • Methods
  • This changed dramatically with the advent of molecular cloning methods. (wikipedia.org)
  • One of the early methods developed for the detection of insert is blue-white screening which allows for identification of successful products of cloning reactions through the colour of the bacterial colony. (wikipedia.org)
  • genome
  • After more than half a century of research and development, the applications of REases have evolved from the cloning of exogenous DNA and genome mapping to more sophisticated applications, such as the identification and mapping of epigenetic modifications and the high-throughput assembly of combinatorial libraries. (neb.com)
  • Next, the clones are sequenced and their position on the genome is verified. (wikipedia.org)