• Molecular
  • The quantity and variety of expressed EC genes identified in this study is sufficient to serve as a global transcriptome dataset for longan EC and to provide more molecular resources for longan functional genomics. (biomedcentral.com)
  • Although many key longan genes and proteins have been cloned and identified, molecular resouces of longan are still limited because genomic and transcriptomic information is lacking. (biomedcentral.com)
  • Although this lack of sequence similarity may mean a reduced functionality of the CASS4 protein, molecular modeling analysis performed by Singh and colleagues using p130CAS/BCAR1 structures as templates suggested an almost identical fold between CASS4 and p130CAS/BCAR1 within their SH3 domains, and substantial similarity within 432-591 residues of CASS4 and 449-610 residues of p130Cas/BCAR1 at the level of secondary and tertiary structures. (wikipedia.org)
  • 1997
  • A C. elegans genomic clone (F52B5.1) was identified when the Ambys-toma kidney NBC (aNBC) was used in a BLAST search against GenBank (Romero et al, Nature 387:409, 1997). (mcmaster.ca)
  • kinase
  • Sequence and model comparison predict 3 potential, N-linked glycosylation sites and several putative, phospho-rylation sites (9 protein kinase C, 9 casein kinase II, and 3 protein kinase A). We subcloned this cDNA into a Xenopus expression vector, transcribed cRNA, and injected the RNA into oocytes for expression. (mcmaster.ca)
  • In contrast to citron, p135 SynGAP, an abundant synaptic Ras GTPase-activating protein that can bind to all three PDZ domains of PSD-95, and Ca 2+ /calmodulin-dependent protein kinase II (CaM kinase II) are concentrated postsynaptically at glutamatergic synapses on glutamatergic neurons. (jneurosci.org)
  • CaM kinase II is not expressed and p135 SynGAP is expressed in less than half of hippocampal GABAergic neurons. (jneurosci.org)
  • For example, citron is expressed at high levels in most thalamic neurons, which are primarily glutamatergic and contain CaM kinase II. (jneurosci.org)
  • In several other brain regions, citron is present in a subset of neurons that can be either GABAergic or glutamatergic and can sometimes express CaM kinase II. (jneurosci.org)
  • nucleotides
  • 1. A recombinant mammalian host cell containing a recombinant or synthetic nucleic acid comprising an open reading frame sequence corresponding to nucleotides 1 to 3315 of SEQ ID NO: 1 that encodes a polypeptide having an amino acid sequence of SEQ ID NO: 2 or an enzymatically active fragment thereof, said open reading frame contained in a human DNA insert of an ˜3.5 kb NotI-BstEII restriction fragment of plasmid pGRN109. (google.com)
  • The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. (wikipedia.org)
  • 1995). "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence" (PDF). (wikipedia.org)
  • domain-containi
  • Indeed, PDZ domain-containing proteins appear to perform a variety of scaffolding functions. (jneurosci.org)
  • Carboxy-terminal to this, they possess an unstructured domain containing multiple SH2 binding site motifs, which when tyrosine-phosphorylated allow interaction with SH2 domain containing proteins. (wikipedia.org)
  • encodes
  • This gene encodes a nuclear protein with three C2H2-type zinc fingers, and functions as a transcriptional repressor. (wikipedia.org)
  • The first and second each contain 7 exons and encode the same full-length protein isoform a (786 amino acids, considered the major isoform), the third one contains 6 exons and encodes a shorter isoform b (732 amino acids) and the fourth one contains 5 exons and encodes the shortest isoform c (349 amino acids). (wikipedia.org)
  • family proteins
  • In activated T cells, Fyb/SLAP associates with Ena/VASP family proteins and is present within biochemical complexes containing WASP, Nck, and SLP-76. (rupress.org)
  • In mammalian cells, overexpression of COOH-terminal fragments of WASP family proteins leads to delocalization of the Arp2/3 complex, resulting in the complete loss of lamellipodia and stress fibers ( Machesky and Insall 1998 ). (rupress.org)
  • For the better studied members of the CAS family (BCAR1 and NEDD9), all of these domains have been defined as crucial for recognition and binding by other proteins, reflecting the primary role of CAS family proteins as cell signaling cascades mediators. (wikipedia.org)
  • It also lacks a YDYVHL sequence at the N-terminal end of the FAT-like carboxy-terminal domain, even though this motif is conserved among the other three CAS family proteins and is an important binding site for the Src SH2 domain. (wikipedia.org)
  • mammalian cells
  • Purified and recombinant proteins TPC2 and TPC3 and recombinant or synthetic oligonucleotides corresponding to those proteins or fragments thereof can be used to detect regulators of telomere length and telomerase activity in mammalian cells and for a variety of related diagnostic and therapeutic pu. (google.com)
  • In an important embodiment, the invention provides oligonucleotide probes and primers, polynucleotide plasmids, peptides, proteins, antibodies, and enzymes relating to genes and gene products that regulate telomere length and telomerase activity in mammalian cells. (google.com)
  • Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. (wikipedia.org)
  • polynucleotide
  • A method for analyzing differential gene expression associated with breast tumor is based on the analysis of the over-expression or under-expression of polynucleotide sequences in a biological sample. (patentsencyclopedia.com)
  • The analysis includes the detection of the over-expression of at least one polynucleotide sequence(s), subsequence(s) or complement(s) thereof selected from predefined polynucleotide sequence sets. (patentsencyclopedia.com)
  • 3. The method according to claim 1 or 2, wherein the analysis consists of detecting the over-expression or the under-expression of all polynucleotide sequences, or complements thereof, in each predefined sequence set. (patentsencyclopedia.com)
  • 4. The method according to claim 1 or 2, wherein said detection of over-expression or under-expression of polynucleotide sequences is performed on polynucleotide sequences from the breast tissue sample. (patentsencyclopedia.com)
  • 5. The method according to claim 1 or 2, wherein said detection of over-expression or under-expression of polynucleotide sequences is performed on at least one polynucleotide array. (patentsencyclopedia.com)
  • 6. The method according to claim 1 or 2, wherein said detection of over-expression or under-expression of polynucleotide sequences is performed by measuring the level of a protein encoded by at least one of the polynucleotide sequences selected from each predetermined set. (patentsencyclopedia.com)
  • 17. A method according to claim 11, 12, 13, or 14 wherein said detection of over-expression or under-expression of polynucleotide sequences is carried out by fluorescence in situ hybridization or immunohistochemistry. (patentsencyclopedia.com)
  • vertebrate
  • The encoded protein is ~40% identical to the vertebrate NBC clones, indicating that along with NBCs and the anion exchangers (AE1-3), ceNBC belongs to the Bicarbonate Transporter Superfamily. (mcmaster.ca)
  • Addition of even 5% CO2 / 33 mM HCO3 (pH 7.5) to ceNBC oocytes, did not elicit a membrane hyperpolarization, typical of our vertebrate NBC clones. (mcmaster.ca)
  • Eukaryotic hyaluronidases are widely distributed, but until recently the only vertebrate enzyme to be cloned was the sperm-specific enzyme, PH20. (springer.com)
  • functional
  • Although the roles of many plant MADS-box proteins have been extensively studied, there are almost no functional studies of them in soybean, an important protein and oil crop in the world. (biomedcentral.com)
  • yeast
  • Using a stringent, high-throughput yeast two-hybrid system, we tested pairwise interactions among the products of approximately 8,100 currently available Gateway-cloned open reading frames and detected approximately 2,800 interactions. (genes2cognition.org)
  • Golgi
  • TMEM59 is a membrane bound protein that is localized to the Golgi apparatus. (wikipedia.org)
  • The precise function of TMEM59 is not known, however it has been demonstrated that expression of TMEM59 protein inhibits Golgi glycosylation of amyloid precursor protein (APP) and blocks APP cleavage by the α- and β-amyloid precursor protein secretases and therefore inhibits formation of the beta amyloid peptide that forms amyloid plaques in Alzheimer's disease. (wikipedia.org)
  • The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. (wikipedia.org)
  • subtraction
  • these studies were mainly focused on calli or embryogenic calli, and involved techniques such as Illumina sequencing, massively parallel signature sequencing (MPSS), EST analysis, microarray analysis, and suppression subtraction hybridization (SSH). (biomedcentral.com)
  • embryogenesis
  • These included genes encoding an unusual group 3 late embryogenesis abundant protein, and a number of other stress-related and DNA repair proteins. (biologists.org)
  • Analyses of unigenes ≥1000 bp revealed 328 embryogenesis-related unigenes as well as numerous unigenes expressed in EC associated with functions of reproductive growth, such as flowering, gametophytogenesis, and fertility, and vegetative growth, such as root and shoot growth. (biomedcentral.com)
  • nuclear
  • Myopodin has actin-bundling activity as shown by formation of latrunculin-A-sensitive cytosolic actin bundles and nuclear actin loops in transfected cells expressing green fluorescent protein-myopodin. (rupress.org)
  • Nuclear export of myopodin is sensitive to leptomycin B, despite the absence of a classical nuclear export sequence. (rupress.org)
  • receptors
  • Synaptic NMDA-type glutamate receptors are anchored to the second of three PDZ (PSD-95/Discs large/ZO-1) domains in the postsynaptic density (PSD) protein PSD-95. (jneurosci.org)
  • Arrestins are regulatory proteins known for connecting G-protein coupled receptors (GPCRs) to clathrin during endocytosis. (wikipedia.org)
  • differentiation
  • The protein encoded by this gene is involved in osteo- and chondrogenic cellular differentiation (the cells which are responsible for the development of bone and cartilage respectively). (wikipedia.org)
  • Genetics
  • We obtained these clones as a generous gift of Dr. Yuji Kohara (Gene Network Lab, Structural Biology Center, National Institute of Genetics, Mishima 411, Japan). (mcmaster.ca)
  • analysis
  • Based on screening of a BAC library with a partial Ve homologous fragment and expression analysis, a V. dahliae -induced gene, Gbvdr6 , was isolated and cloned from the Verticillium wilt-resistant cotton G. barbadense cultivar Hai7124. (frontiersin.org)
  • identification
  • The identification and analyses of mulberry genes involved in diversifying selection, resistance and protease inhibitor expressed in the laticifers will accelerate the improvement of mulberry plants. (science.gov)
  • Prolactin-induced protein has also been used for identification and detection of disseminated breast cancer cells. (wikipedia.org)
  • common structural
  • Although the Z-disc is generally viewed as the site where actin filaments from opposing half-sarcomeres are cross-linked by α-actinin, it has become clear over the past years that more proteins are involved in the formation of the common structural unit of all Z-discs, the Z-unit. (rupress.org)
  • All CAS protein family members have common structural characteristics. (wikipedia.org)
  • expression
  • Protein expression was barely detectable in unsporulated oocysts. (sigmaaldrich.com)
  • 7. The method according to claim 1 or 2, wherein said patient expresses a 2+ level of the HER2 protein on a 0 to 3+ scale in an immunohistochemical assay that determines over-expression of the HER2 protein by measuring binding of a polyclonal antibody to the HER2 protein. (patentsencyclopedia.com)
  • Purification, cDNA cloning, and expression of a new human blood plasma glutamate carboxypeptidase homologous to N-acetyl-aspartyl-alpha-glutamate carboxypeptidase/prostate-specific membrane antigen. (sigmaaldrich.com)
  • GmMADS28 belongs to AGL9/SEP subfamily of MADS-box proteins, localized in nucleus and showed specific expression patterns in floral meristems as well as stamen and petal primordia. (biomedcentral.com)