Clones genome loci genes bacterial. Medical search. Frequent questions

To make the CCMV genome available for precise and rapid gene manipulation techniques, we captured the genomic DNA of CCMV strain Heberling as a bacterial artificial chromosome (BAC). (bvsalud.org)
Comparisons between the genomes of the closely related nematodes Caenorhabditis elegans and Caenorhabditis briggsae reveal high rates of rearrangement, with a bias towards within-chromosome events. (biomedcentral.com)
They described two patients with PNH with an acquired rearrangement of chromosome 12 affecting the HMGA2 gene, which encodes a chromatin remodeling protein, in the PIGA mutant cells [ 12 ]. (annexpublishers.co)
lyrata (Lyre-leaved rock-cress) hypothetical protein (at5g25770) , arath-C7A10.830 Arabidopsis thaliana DNA chromosome 4,gene C7A10.830 No. 2 AT4G36530 , arath-F1N18.12 Arabidopsis thaliana (Mouse-ear cress) f1n18.12 AT1G29840 protein , arath-F1P2.110 Arabidopsis thaliana (Mouse-ear cress). (inrae.fr)
Development of comparative physical gene maps for cattle, sheep, goat and deer by fluorescence in situ hybridisation (FISH) using ovine bacterial artificial chromosome (BAC) clones. (edu.au)
To address this issue, we are employing a bacterial artificial chromosome (BAC) cloning strategy to sequence entire MHC haplotypes from consanguineous cell lines as part of the MHC Haplotype Project. (ox.ac.uk)
de Bary] on chromosome 4 of a dihaploid potato clone (Solanum tubersoum subsp. (hutton.ac.uk)
A bacterial artificial chromosome (BAC) library was constructed from high molecular weight DNA of HR45 and is composed of 33,024 clones. (tamu.edu)
In this study, we constructed the first comprehensive physical map of wheat chromosome arm 5DS, thereby exploring its gene space organization and evolution. (biomedcentral.com)
Here, we provide a chromosome-specific view into the organization and evolution of the D genome of bread wheat, in comparison to one of its ancestors, revealing recent genome rearrangements. (biomedcentral.com)
In the absence of a finished quality genome sequence, insights into wheat genome structure and function have been accumulating through survey sequencing of individual chromosomes or chromosome-specific Bacterial Artificial Chromosome (BAC) libraries. (biomedcentral.com)
The gene is located within the major histocompatibility complex (MHC) on chromosome 6. (genscript.com)
To date, multilocus integration systems in B. subtilis , which is one of the main industrial enzyme producers and a GRAS (generally regarded as safe) microbial host, rely on iterative rounds of plasmid construction for sequential insertions of genes into the B. subtilis chromosome, which is tedious and time consuming. (biomedcentral.com)
Upon transformation of engineered strains with engineered plasmids, strains harboring up to three gene copies integrated into the chromosome formed white colonies because of the removal of the crtMN operon, clearly distinguishable from yellow colonies harboring undesired genetic modifications. (biomedcentral.com)
To circumvent this limitation, the use of a single-crossover integrative vector, which creates direct repeats of the target fragment upon its insertion into the chromosome, offers the possibility of amplifying the integrated plasmid by growing cultures in increasing concentrations of the selective antibiotic and, therefore, amplifying gene dosage in the chromosome [ 10 ]. (biomedcentral.com)
Linkage and comparative mapping studies indicate that the transferred chromosome segment contains a number of candidate genes for hypertension, including genes encoding a brain dopamine receptor and a renal epithelial potassium channel. (jci.org)
These findings demonstrate that BP regulatory gene(s) exist within the differential chromosome segment trapped in the SHR-Lx congenic strain and that this region of chromosome 8 plays a major role in the hypertension of SHR vs. BN-Lx rats. (jci.org)
However, the regulatory region sufficient for accurate expression of SYP remains unknown, thereby preventing development of genetic constructs allowing locus-based Bacterial Artificial Chromosome (BAC) cassettes. (brain-tumor-cancer-information.com)
The resulting plasmids were then integrated into the chromosome at the and loci, respectively, by homologous recombination. (irjs.info)

To demonstrate that genomic loci transferred in this way are functional, the complete human hypoxanthine phosphoribosyltransferase (HPRT) locus contained within a 115-kilobase BAC insert was shown to be expressed when delivered by infection into both a human HPRT-deficient fibroblast cell line and a mouse primary hepatocyte culture derived from Hprt-/- mice. (ox.ac.uk)
26 genomic loci were identified whose haploinsufficiency can modify these CDK8- or CycC-specific phenotypes. (sdbonline.org)

Bacterial artificial chromosomes (BACs) and P1-based artificial chromosomes (PACs) have proved excellent tools for the human genome sequencing projects. (ox.ac.uk)

In addition, inspired by Barbara McClintock's work on jumping genes in maize, Fink and his postdocs discovered that yeast transposable elements, known as Ty elements, move around the chromosomes through retrotransposition via an RNA intermediate. (genestogenomes.org)
The construction of BAC-based physical maps of individual chromosomes reduces the complexity of this allohexaploid genome, enables elucidation of gene space and evolutionary relationships, provides tools for map-based cloning, and serves as a framework for reference sequencing efforts. (biomedcentral.com)

The recent completion of the human genome sequence allows genomics research to focus on understanding gene complexity, expression, and regulation. (ox.ac.uk)
To assess whether this pattern is true of nematodes in general, we have used genome sequence to compare two nematode species that last shared a common ancestor approximately 300 million years ago: the model C. elegans and the filarial parasite Brugia malayi . (biomedcentral.com)
Potential C. elegans orthologs for II of the 12 protein-coding genes predicted in the B. malayi sequence were identified. (biomedcentral.com)
Comparison of B. malayi BAC-end genome survey sequence to C. elegans also revealed a bias towards intrachromosome rearrangements. (biomedcentral.com)
Kent and Zahler [ 9 ] compared 8.1 megabases (Mb) of fragmentary C. briggsae sequence derived from sequenced cosmid clones to C. elegans and derived a mean syntenic fragment length of 8.6 klobases (kb), or approximately 1.8 genes (there is one gene per 5 kb in C. elegans ) [ 13 ]. (biomedcentral.com)
Here we obtained a well-resolved polymorphism type of intergenic spacer (IGS) sequence phylogeny of B. burgdorferi sensu stricto clonal complexes (corresponding to the ospC- A and -B groups) is associated by using multilocus sequence typing at housekeeping loci with hematogenous dissemination in patients with early as well as loci under adaptive evolution. (cdc.gov)
Advancement in identification of MHC genes that confer susceptibility to disease requires greater knowledge of sequence variation across the complex. (ox.ac.uk)
We have defined the complete gene, splice variant, and sequence variation contents of all three haplotypes, comprising over 259 annotated loci and over 20,000 single nucleotide polymorphisms (SNPs). (ox.ac.uk)
Whole genome sequences of these isolates were determined and analysed to compute bacterial multilocus sequence types and plasmid replicon types, infer phylogenetic relationships, and identify antimicrobial resistance and virulence genes. (biomedcentral.com)
The following PBX2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. (origene.com)
Generation of Mice A BAC clone containing approximately 121 kb and 36 kb of 5′ and 3′ Rabbit Polyclonal to MYH14 DNA flanking the locus was modified by insertion of a cassette VX-222 to replace the sequence spanning intron 1 downstream of to exon 7 of locus by VX-222 homologous recombination. (brain-tumor-cancer-information.com)

Highly duplicated and polymorphic regions of the human genome such as the MHC are, however, somewhat refractory to some whole-genome analysis methods. (ox.ac.uk)
Whole-genome transposon-mutagenesis (TM) followed by identification of insertion sites is one of the most practical and frequently used experimental approaches to screen for essential bacterial genes [ 6 - 8 ]. (biomedcentral.com)
Whole-genome sequencing used to investigate a nationwide outbreak of listeriosis caused by ready-to-eat delicatessen meat, Denmark, 2014. (cdc.gov)

To resolve phylogenetic relationships among ries of bacterial clonal complexes by using concatenated isolates, we sequenced 68 isolates from Europe and North sequences of housekeeping genes when within-loci and America at 1 chromosomal locus (16S-23S ribosomal RNA between-loci recombinations are infrequent ( 5 ). (cdc.gov)
The genetic homogeneity of ospC -A isolates was confi rmed agent, Borrelia burgdorferi , a spirochete that parasitizes by sequences at 6 additional chromosomal housekeeping vertebrates, is transmitted by hard-bodied ticks through- loci ( gap, alr, glpA, xylB, ackA, and tgt ). (cdc.gov)
Despite the advent of next-generation sequencing technologies, the above mentioned attributes of the wheat genome have rendered the assembly of genomic sequences extremely difficult. (biomedcentral.com)
However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. (origene.com)
However, genome sequences of predict the presence of a number of LuxI-LuxR family proteins. (irjs.info)

Variant of PBX2 gene in the 6p21.3 asthma susceptibility locus is associated with allergic rhinitis in Chinese subjects. (genscript.com)
A genome-wide association study identifies 2 susceptibility Loci for Crohn's disease in a Japanese population. (genscript.com)
Genome-wide association study identifies eight new susceptibility loci for atopic dermatitis in the Japanese population. (genscript.com)
The findings from the present study that a SNP of the PBX2 gene in the MHC region (at 6p21.3) was associated with allergic rhinitis (AR) in allele and genotypic analysis suggests a new susceptibility gene for AR in the MHC region and another gene associated with both asthma and AR in Chinese subjects. (genscript.com)
Susceptibility loci for lupus: a guiding light from murine models? (jci.org)

The major burden of iuc and iro amongst the genomes analysed was due to two linked lineages ( iuc1 / iro1 74% and iuc2 / iro2 14%), each carried by a distinct non-self-transmissible IncFIB K virulence plasmid type that we designate KpVP-1 and KpVP-2. (biomedcentral.com)
spacer) and 3 plasmid loci ( ospC , dbpA , and BBD14). (cdc.gov)
Second, a single CRISPR-Cas9-based plasmid carrying a highly specific single guide RNA (sgRNA) targeting crtMN operon and a changeable editing template was constructed to facilitate simultaneous insertion of multiple gene-copies through homology-directed repair (HDR). (biomedcentral.com)
To this end, a traditional approach involves the introduction of replicative plasmids, where the level of gene expression is dictated by the copy number of the plasmid in the cells [ 9 ]. (biomedcentral.com)
Commonly used conditions for ampicillin resistant plasmid clones. (dnasu.org)
A plasmid that constitutively expresses was constructed by cloning of the genes into the pBBR1-MCS5 vector (16) and launched into strains by electroporation. (irjs.info)

Mutations in FOXP2 are among several (26 genes plus 2 intergenic) loci which correlate to ADHD diagnosis in adults - clinical ADHD is an umbrella label for a heterogeneous group of genetic and neurological phenomena which may result from FOXP2 mutations or other causes. (wikipedia.org)
Botstein is honored for contributions that include developing methods for defining genetic pathways, mapping genomes, and analyzing gene expression. (genestogenomes.org)
Here, we examined the genetic diversity, distribution and mobilisation of iuc and iro loci amongst 2503 K . pneumoniae genomes using comparative genomics approaches and developed tools for tracking them via genomic surveillance. (biomedcentral.com)
The antimicrobial resistance (AMR) and/or virulence determinants possessed by the associated bacteria are generally found on mobile genetic elements (MGEs) that transmit between K . pneumoniae cells via horizontal gene transfer (HGT) [ 4 ]. (biomedcentral.com)
Ixodes ricinus ticks ( 11 ) and is carried by a large variety of ize genetic variations of natural populations of a bacterial hosts, including birds and small- to medium-sized mam- pathogen ( 1 , 2 ). (cdc.gov)
2006. Construction of a 10,000 marker ultradense genetic recombination map of potato: providing a framework for accelerated gene isolation and genomewide physical map . (hutton.ac.uk)
Ten plasmids encoding either KPC-2, NDM-1, or NDM-7 carbapenemases were characterized, including novel plasmids with increased resistance gene load and a novel genetic environment for bla KPC-2 gene. (biorxiv.org)
Despite recent advances in genetic engineering tools for effectively regulating and manipulating genes, efficient simultaneous multigene insertion methods have not been established in Bacillus subtilis . (biomedcentral.com)
However, the use of antibiotic resistance markers limits their use in industrial applications due to both the genetic instability of many recombinant plasmids in the absence of selection and the restrictions and concerns derived from the massive abuse of antibiotics, promoting the emergence of bacterial resistance. (biomedcentral.com)
[ 2 ] Three biovars (with minor genetic variations) have been identified within the Y pestis clone: Antiqua, Medievalis, and Orientalis. (medscape.com)
The virulence of this bacterium results from the 32 Y pestis chromosomal genes and two Y pestis -specific plasmids, constituting the only new genetic material acquired since its evolution from its predecessor. (medscape.com)
Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)

genome encodes one of each group I, II, and III sigma factors represented by SigA, SigB, and SigF, respectively, and 25 of group IV sigma factors (Kapopoulou et?al. (healthyguide.info)
This gene encodes a ubiquitously expressed member of the TALE/PBX homeobox family. (genscript.com)

Although the bacterial translocase has been characterized in other intercellular pathogens such as Chlamydia trachomatis and Rickettsia prowazeki, this is the first characterization of an ATP/ADP translocases in an obligate-intracellular plant pathogen and, given the conservation of the ATP uptake mechanism, may represent an evolutionary link between plant and animal pathogens. (usda.gov)
Of the bacterial ATP/ADP translocases characterized thus far, all have been found in endosymbionts of protozoa or pathogens of higher order animals including humans. (usda.gov)
The work package focusing on molecular studies generated new evidence about the changes effected by antibiotic therapy on commensal organisms or opportunistic pathogens in the oropharyngeal, nasal and gastro-intestinal flora and study AMR mechanisms and the dissemination of successful clones of fluoroquinolone-resistant, carbapenem-resistant or extended-spectrum beta-lactamase harboring Gram-negative bacteria, MRSA and fluoroquinolone-resistant viridans streptococci. (europa.eu)
Multidrug and carbapenem-resistant K. pneumoniae (CR- Kp ) are considered critical threats to global health and key traffickers of resistance genes to other pathogens. (biorxiv.org)
Targeting of novel essential pathways is expected to play an important role in the discovery of new antibacterial agents against bacterial pathogens, such as Pseudomonas aeruginosa , that are difficult to control because of their ability to develop resistance, often multiple, to all current classes of clinical antibiotics. (biomedcentral.com)
Profile and Fate of Bacterial Pathogens in Sewage Treatment Plants Revealed by High-Throughput Metagenomic Approach. (cdc.gov)

Mapping quantitative trait loci (QTL) in cattle for meat quality, particularly fat and muscle metabolic traits. (edu.au)
Detection of a quantitative trait locus for both foliage and tuber resistance to late blight [Phytophthora infestans (Mont. (hutton.ac.uk)
A major quantitative trait loci (QTL) conditioning common bacterial blight (CBB) resistance in common bean (Phaseolus vulgaris L.) lines HR45 and HR67 was derived from XAN159, a resistant line obtained from an interspecific cross between common bean lines and the tepary bean (P. acutifolius L.) line PI319443. (tamu.edu)

FOXP2 is a member of the forkhead box family of transcription factors, proteins that regulate gene expression by binding to DNA. (wikipedia.org)
We further characterized the dynamics of CCMV gene expression and found that viral proteins cluster into five distinct temporal classes. (bvsalud.org)
The protein product of the translocase gene demonstrated characteristics typical for members in this family of proteins, including 12 transmembrane helices and an isoelectric point of 9.4. (usda.gov)
They cloned the HIS4 locus, yeast's best candidate for an operon and showed that it in fact encoded a single multifunctional protein, rather than multiple proteins under common regulation. (genestogenomes.org)
2004). Bopindolol malonate Overexpression of in resulted in the differential regulation of many cell wall\associated proteins and other genes involved in the biosynthesis and degradation of surface polysaccharides and lippolysaccharides, believed to play important roles in host\pathogen interactions (Williams et?al. (healthyguide.info)
A family of LIC vectors for high-throughput cloning and purification of proteins. (dnasu.org)

Fink is recognized for the discovery of principles central to genome organization and regulation in eukaryotic cells. (genestogenomes.org)
To also target low expressed essential genes, we included some variant steps that were expected to overcome the non-stringent regulation of the promoter carried by the expression vector used for the shotgun antisense libraries. (biomedcentral.com)
Regulation of bacterial virulence gene expression by the host environment. (jci.org)

Forkhead box protein P2 (FOXP2) is a protein that, in humans, is encoded by the FOXP2 gene. (wikipedia.org)
The FOXP2 gene region acts as a transcription factor for the forkhead box P2 protein. (wikipedia.org)
Transcription factors affect other regions, and the forkhead box P2 protein has been suggested to also act as a transcription factor for hundreds of genes. (wikipedia.org)
The A and B clones of Borrelia burgdorferi sensu stric- maintenance of these clonal complexes in natural bacterial to, distinguished by outer surface protein C ( ospC ) gene populations ( 3 , 4 ). (cdc.gov)
Protein expression attributed to a single copy of the integrated gene usually results in lower yields of product compared to the use of high copy-number vectors. (biomedcentral.com)
A higher protein intake correlated with a lower Bacteroides abundance and an altered abundance of several other bacterial taxa. (uni-frankfurt.de)
(B) Expressions of RNASET2 in human tissues were analyzed in the Human Protein Atlas database, and the resulting transcript expression values, denoted normalized expression (NX), were calculated for each gene in every sample. (frontiersin.org)

When Fink's career began, the discovery of DNA and the molecular biology revolution had changed little about the study of yeast genetics because there was no method to introduce DNA into a yeast cell, a critical step for tinkering with genes in vivo . (genestogenomes.org)
Her research interests are in livestock molecular genetics and include the genome organization of Bovidae, genome mapping in Bovidae and genetics of lipid metabolism in cattle. (edu.au)
2006. Assessing the potential of regions of the nuclear and mitochondrial genome to develop a 'molecular tool box' for the detection and characterization of Phytophthora species . (hutton.ac.uk)
Four molecular markers tightly linked to this QTL have been identified suitable for marker assisted selection and physical mapping of the resistance gene. (tamu.edu)
Application of Molecular Typing Results in Source Attribution Models: The Case of Multiple Locus Variable Number Tandem Repeat Analysis (MLVA) of Salmonella Isolates Obtained from Integrated Surveillance in Denmark. (cdc.gov)

Three methods including MetaCore analysis for disease and pathway correlations, concordance analysis between array-CGH database and the expression array database, and literature search for copy number variation genes were performed to select novel lung cancer candidate genes. (biomedcentral.com)
Moreover, we showed that the expression of some candidate genes (E2F3, CDKAL1 and Sox4) in the 6p22.3 region is highly correlated with the chromosomal amplification. (oncotarget.com)

In addition, long-read sequencing of one representative XDR ST235 isolate identified an integron carrying multiple resistance genes (including bla VIM-2), with differences in gene composition and synteny from the P. aeruginosa class 1 integrons described previously. (who.int)

P. aeruginosa isolates referred to the ARSP, epidemic clone. (who.int)
Lyme disease is a multisystem infection, with infl am- tinents, and isolates of this clone were uniform in DNA se- matory complications that commonly affect the skin, joints, quences, which suggests a recent trans-oceanic migration. (cdc.gov)
High resistance to most of the antibiotics tested was observed among the isolates, five ST25, three ST11, one ST45, and one ST505, which harbored a total of 44 plasmids, many of them predicted to be conjugative and carrying genes conferring resistance to a variety of antibiotic, metals, and disinfectants. (biorxiv.org)
The most abundant carbapenemase gene family in the K. pneumoniae isolates (33/39) was bla OXA-232 , with bla NDM-1 additionally identified in 19 of them. (biomedcentral.com)

100 kilobases, a technique that may prove useful for analyzing gene expression from the human genome. (ox.ac.uk)

We describe a system to rapidly and efficiently deliver and express BAC and PAC library clones in human and mouse cells by converting them into infectious amplicon vectors. (ox.ac.uk)

Thus, BAC-cloned CCMV can serve as a powerful tool for systematic approaches in comparative functional genomics, exploiting the close phylogenetic relationship between CCMV and HCMV. (bvsalud.org)

2010). However, we earlier demonstrated that, is conserved in all the mycobacterial species analyzed and proposed that apart from regulating the expression of virulence genes in pathogenic mycobacteria, SigF is likely to play more roles in mycobacterial physiology (Singh and Singh 2008). (healthyguide.info)

When compared to the complete genome of C. elegans , evidence for conservation of long-range synteny and microsynteny was found. (biomedcentral.com)

McClintock B . The origin and behavior of mutable loci in maize. (microbiologyresearch.org)

Two groups have attempted to assess genome rearrangement rates and modes in comparisons between these two species. (biomedcentral.com)
There is an unusual expansion of several genes which have acquired many paralogs unlike in other mycobacterial species (Waagmeester et?al. (healthyguide.info)
and 16 were "novel" essential genes with no homologs reported to have an essential role in other bacterial species. (biomedcentral.com)
Genomes of cryptic chimpanzee Plasmodium species reveal key evolutionary events leading to human malaria. (cdc.gov)

Centre for Genomic Pathogen Surveillance, Wellcome Genome Campus, Hinxton, England, United Kingdom of Great Britain and Northern Ireland. (who.int)

The novel technology expands the genome-editing toolset for B. subtilis and means a substantial improvement over current methodology, offering new application possibilities that we envision should significantly boost the development of B. subtilis as a chassis in the field of synthetic biology. (biomedcentral.com)

He started working with the organism as a Yale graduate student spurred by the recent discovery in bacteria of operons-clusters of genes with related function controlled by a shared regulatory element transcribed into a single messenger RNA. (genestogenomes.org)
This study also identified 43 growth-impairing inserts carrying multiple loci targeting 105 genes, of which 25 have homologs reported as essential in other bacteria. (biomedcentral.com)

The population of P. aeruginosa was diverse, with clonal expansions of XDR genomes belonging to MLSTs ST235, ST244, ST309 and ST773. (who.int)
Recently, a second clonal event involving the HMGA2 gene was reported in some PNH cases, and over expression of this gene was postulated to promote proliferation of the mutated clone. (annexpublishers.co)
Unlike dysplastic clones in myelodysplastic syndrome (MDS), which harbour intrinsic defects that predispose the cells to clonal evolution, external factors, such as pressure exerted by the immune system, have for a long time been speculated to be the main causes for clonal escape in PNH [ 5 ]. (annexpublishers.co)

We conclude that PCR-based enrichment of transposon insertions in a TnSeq assay is reliable, but researchers interested in profiling putative essential genes should carefully weigh the number of amplification cycles employed in their library preparation protocols. (microbiologyresearch.org)

however, individual strains vary in their virulence potential due to the presence of mobile accessory genes. (biomedcentral.com)
Concerningly, iuc has also been reported in MDR strains in the hospital setting, where it was associated with increased mortality, highlighting the need to understand, detect and track the mobility of these virulence loci in the K . pneumoniae population. (biomedcentral.com)
This study provides a framework for identifying and tracking these important virulence loci, which will be important for genomic surveillance efforts including monitoring for the emergence of hypervirulent MDR K . pneumoniae strains. (biomedcentral.com)
MATERIALS AND METHODS Bacterial strains, plasmids, and culture conditions. (irjs.info)
Bacterial strains and plasmids used in this study are outlined in Table ?Table1.1. (irjs.info)
Bacterial strains and plasmids used in this study strains? (irjs.info)

Furthermore, we used 16S ribosomal RNA gene sequencing to detect associations with different abundances of the gut microbiota with dietary patterns. (uni-frankfurt.de)

DNA sequencing confirmed the integrity of our clones and led to the identification of two polymorphic loci and a deletion-prone region within the CCMV genome. (bvsalud.org)

In-framework deletions in the and genes Rabbit Polyclonal to TEAD1 were constructed by overlapping PCR of flanking regions of the prospective genes and cloning into the pWM91 suicide vector (20). (irjs.info)

These PCR-based and PCR-free experiments demonstrate that, overall, PCR amplification does not significantly bias the results of TnSeq insofar as insertions in the majority of genes represented in our library were similarly detected regardless of PCR cycle number and whether or not PCR amplification was employed. (microbiologyresearch.org)
For example, even if comprehensive insertion libraries are produced, it is inevitable that some genes, especially the shortest ones, could elude insertion and be spuriously annotated as essential, while transposon insertions that occur at gene ends and do not fully inactivate the function could lead to genes being incorrectly classified as non-essential. (biomedcentral.com)

By activating the expression of genes whose products are required for metamorphosis, ecdysone and EcR-USP are essential for the reorganization of flies' body plans before emerging from pupal cases as adults. (sdbonline.org)

Genomic DNA copy number variation is a hallmark of cancer and can lead to alteration in the expression and functions of genes residing within the affected chromosomal region [ 5 ]. (biomedcentral.com)
This revealed no aberration of the chromosomal region containing the HMGA2 gene in the cohort, and HMGA2 expression was surprisingly only detectable in a single case. (annexpublishers.co)

2010). strain was attenuated for virulence in a mouse infection model despite persistence at high bacterial load in lungs compared with the isogenic wild type (Geiman et?al. (healthyguide.info)

Due to an inability to genetically manipulate Las at the present time, the translocase (tlc) open reading frame was cloned and expressed in Escherichia coli to determine if this putative gene was in fact functional. (usda.gov)
For and transcriptional reporter fusions were constructed by PCR, amplifying the internal fragment of and intact 5 (including its putative promoter region), and these fragments were cloned into pVIK112 (15). (irjs.info)

Fink and his students showed that many genes related to amino acid biosynthesis were controlled by a single master regulator-the transcription factor Gcn4p. (genestogenomes.org)
In particular, gene clusters encoding the biosynthesis of siderophores aerobactin ( iuc ) and salmochelin ( iro ) are associated with invasive disease and are common amongst hypervirulent K . pneumoniae clones that cause severe community-associated infections such as liver abscess and pneumonia. (biomedcentral.com)

Thus, although PIGA mutations are established as pathogenetic drivers of PNH, larger deletions of the locus are rare. (annexpublishers.co)

A single deletion affecting the PIGA locus was found, but no other relevant or recurrent abnormalities were detected. (annexpublishers.co)
Analysis of the gene space of 5DS suggested an increasing gradient of genes organized in islands towards the telomere, with the highest gene density of 5.17 genes/Mb in the 0.67-0.78 deletion bin, 1.4 to 1.6 times that of all other bins. (biomedcentral.com)
B subtilis gene deletion of yoqG. (addgene.org)

To achieve maximum expression of a particular gene in Bacillus , it is highly desirable to amplify the copy number of the gene of interest [ 8 ]. (biomedcentral.com)

Evolutionary History of the Global Emergence of the Escherichia coli Epidemic Clone ST131. (cdc.gov)

However, other genes are necessary for human language development, and a 2018 analysis confirmed that there was no evidence of recent positive evolutionary selection of FOXP2 in humans. (wikipedia.org)
Oxford: Lion, 2018) and "Are We Slaves to Our Genes? (tamu.edu)

Whereas shared ancestry and functional constraints will increase conservation of linkage and synteny between taxa, rearrangement events will tend to randomize gene order over time. (biomedcentral.com)

We suggest that intrachromosomal rearrangement is a major force driving chromosomal organization in nematodes, but is constrained by the interdigitation of functional elements of neighboring genes. (biomedcentral.com)

Methods: The individual taxonomic microbiota composition of fecal samples from 90 NAFLD patients and 21 healthy controls was analyzed using 16S rRNA gene sequencing. (uni-frankfurt.de)
Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis. (cdc.gov)

Transposon-based approaches to identify essential bacterial genes. (microbiologyresearch.org)

The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching has made luciferase a common choice in assays ranging from use as a reporter gene in vitro and in vivo to use in biosensors. (addgene.org)
To investigate the response of LuxS01 and wild type strain to exogenously added AI-2, cells from Venetoclax in vitro experiments A and B were harvested in late exponential phase for RNA extraction and microarray gene expression analysis. (hsdpathway.com)

A single translocase encoding gene was identified through an in silico analysis of the recently completed Las genome. (usda.gov)
In addition, CISH analysis of patients indicated that copy number amplification indeed occurred for ARHGAP19 and ZNF322A genes in lung cancer patients. (biomedcentral.com)
Second, fluorescence in situ hybridization was conducted for the region containing the HMGA2 gene in addition to expression analysis, to study the recurrence of changes at this locus. (annexpublishers.co)
Insights into H 2 O 2 -induced signaling in Jurkat cells from analysis of gene expression. (otago.ac.nz)

In protostomes, comparative studies of the genomes of closely related dipterans ( Drosophila sp. (biomedcentral.com)

The size of individual BAC clone inserts ranges from 30 kb to 280 kb with an average size of 107 kb. (tamu.edu)

We identify the lipase and its gene cosegregates with the low-/high-lipase trait, providing breeders a marker to rapidly identify potent elite genitors and introgress the trait into major cultivars. (nature.com)
lyrata (Lyre-leaved rock-cress) Triacylglycerol lipase 2 gene At5g1418 MUA22.18 MUA22.19 MYZUS PERSICAE-INDUCED LIPASE 1 , arath-Q8LPF5 Arabidopsis thaliana (Mouse-ear cress) Arabidopsis lyrata subsp. (inrae.fr)

Such a segment in the tumor genome may harbor either oncogenes or tumor suppressor genes depending on whether they are present in increased or decreased copy number, respectively. (biomedcentral.com)

Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study. (genscript.com)

Furthermore, this analyses confirmed the positive roles of CDK9 and Yorkie in regulating Mad-dependent gene expression in vivo. (sdbonline.org)

gene symbol (e.g. (genscript.com)

Somatic, inactivating PIGA mutations in haematopoietic stem cells, followed by an unknown autoimmune selection process in favor of the mutated clone, are thought to be important events in the pathogenesis of paroxysmal nocturnal hemoglobinuria (PNH). (annexpublishers.co)
Luciferase-containing plasmids are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression. (addgene.org)
Delineation and characterisation of mutations in the factor IX gene of haemophilia B patients. (edu.au)
Reads mapping to the junctions are enumerated thus providing information on the number of transposon insertion mutations in each individual gene. (microbiologyresearch.org)

The SigF\anti\SigF interaction was duly confirmed using bacterial two\hybrid system and pull down assay. (healthyguide.info)

Cancer-related genes show racial differences. (biomedcentral.com)

Anatomy2

Organisms2

Chemicals and Drugs2

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes29

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science4

Chromosome19

Genomic loci2

Artificial chromosomes1

Chromosomes2

Sequence12

Whole genome3

Sequences5

Susceptibility5

Plasmid6

Genetic12

Encodes2

Pathogens6

Quantitative3

Proteins6

Regulation3

Protein7

Molecular5

Candidate genes2

Resistance genes1

Isolates4

Human genome1

Vectors1

Genomics1

Virulence genes1

Complete genome1

Maize1

Species4

Pathogen1

Biology1

Bacteria2

Clonal3

Essential genes1

Strains6

Ribosomal1

Polymorphic1

Rabbit Polyclonal1

Insertions2

Expression of ge1

Chromosomal region2

Infection1

Putative2

Biosynthesis2

Deletions1

Deletion3

Bacillus1

Emergence1

20182

Taxa1

Rearrangement1

RRNA2

Approaches1

Vitro2

Analysis4

Comparative1

Inserts1

Lipase2

Tumor1

Variation1

Vivo1

Symbol1

Mutations4

Assay1

Differences1