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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells' (2007), by Junying Yu et al. (asu.edu)
On 2 December 2007, Science published a report on creating human induced pluripotent stem (iPS) cells from human somatic cells: "Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. (asu.edu)
Since human induced pluripotent stem cell (iPSC)-platelet products can be supplied independently from the donor, it is expected to complement current platelet products. (biomedcentral.com)
Induced pluripotent stem cells (iPSC) express genes associated with pluripotency, have the capacity for infinite expansion, and can differentiate into cells from all three germ layers. (biomedcentral.com)
Among PSCs, the donors available for induced pluripotent stem cells (iPSCs) are greatest, providing a potentially universal cell source for all types of cell therapies including cancer immunotherapies using natural killer (NK cells). (molcells.org)
Among stem cells, induced pluripotent stem cells (iPSCs) have a number of features that make them ideal as the starting source. (molcells.org)
Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
They are 3-dimensional, complex, multicellular structures that can be derived from patient specific human induced pluripotent stem cells (hiPSCs). (nature.com)
First year progress on grant ID1-06557, " Generation and Characterization of High-Quality, Footprint-Free Human Induced Pluripotent Stem Cell (iPSC) Lines From 3000 Donors to Investigate Multigenic Disease" has met all agreed-upon milestones. (ca.gov)
The goal of CIRM Grant ID1-06557 is to generate high quality induced pluripotent stem cells (iPSC) from blood and skin samples from 3000 donors, many of whom suffer from untreatable medical conditions, and place them in a Repository accessible to scientists around the world. (ca.gov)
Human induced pluripotent stem cell-derived mesenchymal stem cells (iPS-MSCs) are emerging as attractive options for use in cell replacement therapy, but their effect in kidney diseases remains unknown. (oncotarget.com)
Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)

We used primary acute myeloid leukemia (AML) samples and human AML cell lines to investigate the regulatory mechanisms of autophagy and its role in AML differentiation. (hindawi.com)
To induce differentiation and maturation of the immortalized cell lines, terminating the HPV-E6/E7 expression through a gene induction system has been believed to be essential. (lu.se)
Here, we report that erythroid cell lines established from human bone marrow using simple expression of HPV-E6/E7 are capable of normal erythroid differentiation, without turning gene expression off. (lu.se)
Upon differentiation, other ELLU clones shift from fetal to adult hemoglobin expression, giving rise to more mature cells. (lu.se)
Our findings propose that it is not necessary to employ gene induction systems to establish immortalized erythroid cell lines sustaining differentiation potential and describe novel cellular characteristics for desired functionally competent clones. (lu.se)
Clinical translation of human pluripotent stem cells (hPSCs) requires advanced strategies that ensure safe and robust long-term growth and functional differentiation. (labroots.com)
This study describes for the first time, a differentiation system that does not require co-culture with animal cells or products, thus allowing the production of pathogen-free RPE cells suitable for transplantation into patients. (news-medical.net)
Mitogen-activated protein kinases (MAPK)3, such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p MAPK, are involved in signal transduction from the cell surface to the nucleus and regulate cellular processes, including proliferation, differentiation, cell growth arrest and apoptosis, which are also important for the promotion phase of carcinogenesis. (beauty104.com.tw)
Deletion of HP1β, but not HP1α, in ESCs provokes a loss of the morphological and proliferative characteristics of embryonic pluripotent cells, reduces expression of pluripotency factors and causes aberrant differentiation. (biomedcentral.com)
However, in differentiated cells, loss of HP1β has the opposite effect, perturbing maintenance of the differentiation state and facilitating reprogramming to an induced pluripotent state. (biomedcentral.com)
We demonstrate an unexpected duality in the role of HP1β: it is essential in ESCs for maintaining pluripotency, while it is required for proper differentiation in differentiated cells. (biomedcentral.com)
This unique duality makes them an attractive system for potential regenerative medicine and cell therapies, but also for differentiation studies in vitro and for modeling diseases. (biomedcentral.com)
Here we show that cell differentiation suppresses tumor formation. (amegroups.org)
Cells were transfected with the early B-cell factor-2 (EBF2) to activate progenitor cell differentiation and gauged the tumorigenic potentials of the derived cell subsets. (amegroups.org)
Here we show that the brown adipose differentiation gene, EBF2, activates differentiation-associated signals in the cancer progenitor cells in culture and CSC transplantation models. (amegroups.org)
This study reveals a previously unrecognized function of EBF2 in regulating PDAC progenitor cell differentiation and tumor suppression. (amegroups.org)
The term stem cell can be defined by two very important qualities: the cell has the ability to self-renew and, in a more general sense, the cell has not completed differentiation into its final state. (thefutureofthings.com)
This general definition includes a wide variety of cells with varying degrees of differentiation potential. (thefutureofthings.com)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
In CaCo-2 colonic epithelial cells, the expression of this gene increases with cell differentiation. (aacrjournals.org)
The basal cells (BC) are the stem/progenitor cells of the SAE, responsible for the differentiation into intermediate cells and ciliated, club and mucous cells. (biomedcentral.com)
The resulting cell line (hSABCi-NS1.1) was characterized by RNAseq, TaqMan PCR, protein immunofluorescence, differentiation capacity on an air-liquid interface (ALI) culture, transepithelial electrical resistance (TEER), airway region-associated features and response to genetic modification with SPDEF. (biomedcentral.com)
The immortalized hSABCi-NS1.1 cell line has diverse differentiation capacities and retains SAE features, which will be useful for understanding the biology of SAE, the pathogenesis of SAE-related diseases, and testing new pharmacologic agents. (biomedcentral.com)
The HT-29-C1.19A cells can be used for investigation of intestinal cell differentiation. (mbpinc.net)
Restoration by Polyethylene Glycol of Characteristics of Intestinal Differentiation in Subpopulations of the Human Colonic Adenocarcinoma Cell Line HT29. (mbpinc.net)
In vivo differentiation to cells from all three germlines was tested using a teratoma assay. (biomedcentral.com)
In vitro differentiation of GS-2 iPSCs into hematopoietic stem and progenitor cells was done using Op9 feeder layers and specified media. (biomedcentral.com)
Differentiation capacity into cells from all three germ layers was confirmed using the teratoma assay. (biomedcentral.com)
Accordingly, scientists have developed protocols for the expansion, genetic modification and differentiation of stem cells to NK cells. (molcells.org)
Gain and loss of function studies have indicated that miRNAs play a critical role in the regulation of all key biological functions such as development, cell proliferation, cell differentiation, and apoptosis [ 3 , 4 ]. (hindawi.com)
Differentiation -The process whereby an unspecialized early embryonic cell acquires the features of a specialized cell such as a heart, liver, or muscle cell. (cellmedicine.com)
Directed differentiation -Manipulating stem cell culture conditions to induce differentiation into a particular cell type. (cellmedicine.com)
Embryonic stem cell line -Embryonic stem cells, which have been cultured under in vitro conditions that allow proliferation without differentiation for months to years. (cellmedicine.com)
This co-culture system allows investigation into the effects of myocyte derived soluble factors uponepicardial EMT and mesenchymal cell differentiation. (arizona.edu)
The exact process of differentiation is not yet understood and although embryonic stem cells can, in principle, provide for all human tissue, scientists are some way from controlling the process. (spiked-online.com)
Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
It demonstrated that genes inactivated during tissue differentiation can be completely re-activated by a process called nuclear reprogramming: the reversion of a differentiated nucleus back to a totipotent status. (biomedcentral.com)
Various biological events associated with bone healing on implant surfaces can be investigated separately using appropriate cell cultures, such as cell adhesion, proliferation and differentiation, as well as the production and mineralization of extracellular matrix. (bvsalud.org)
The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
Rarely, mucinous differentiation or squamous cell carcinoma is seen. (medscape.com)

NEW YORK, September 21, 2003 - New research from Memorial Sloan Kettering Cancer Center (MSKCC), Cornell University, and The University of Connecticut describes a novel way of producing therapeutic nerve cells that can cure mice with Parkinson's-like disease. (mskcc.org)
With therapeutic cloning, or the creation of banks of stem cells generated using parthenogenesis (perhaps as few as 40-400 lines), a limitless supply of immune-matched cells could be generated for most of the population. (news-medical.net)
HLA-A disruption from donor cells is a step toward generating allogeneic cells as an off-the-shelf therapeutic. (ashpublications.org)
A number of large biotech companies and scientists are looking toward stem cells as the basis for a therapeutic solution to cure such illnesses as blindness, diabetes and spinal cord injuries. (thefutureofthings.com)
The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
5. All of abm 's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. (mbpinc.net)
abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). (mbpinc.net)
A renewable, tissue culture source of human cells capable of differentiating into a wide variety of cell types would have broad applications in basic research and therapeutic techniques. (spiked-online.com)
One of the most promising techniques is what is known as therapeutic cloning. (spiked-online.com)
Recent findings have provided pre-clinical proof-of-concept for the potential therapeutic utility of the melanoma stem cell concept. (stemcellsciencenews.com)
Therefore, melanoma stem cell-directed therapeutic approaches represent promising novel strategies to improve therapy of this arguably most virulent human cancer. (stemcellsciencenews.com)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
A collaborative research effort led by scientists and engineers at the University of Pennsylvania and Northwestern University led to the development of FateMap, a framework that combines DNA barcoding, single-cell RNA sequencing (scRNA-seq), and computational analysis to study the 'fate' or outcomes after exposing cancer cells to therapeutic drugs. (cbirt.net)
Upon exposure to therapeutic drugs, resistant clones form resistant colonies and proliferate. (cbirt.net)
Through a strategic integration process, these barcoded cells are subjected to therapeutic drug treatments, with a specific focus on this study's use of vemurafenib for targeted therapy. (cbirt.net)
Britain s House of Lords final approval of therapeutic human cloning and embryonic stem cells research has intensified the battle for ascendancy between adult and embryonic stem cells. (i-sis.org.uk)
Embryonic cell lines and autologous embryonic stem cells generated through therapeutic cloning have also been proposed as promising candidates for future therapies. (techxplore.com)
Therapeutic cloning possesses enormous potential for revolutionizing medical and thera- peutic techniques. (who.int)
This is therapeutic cloning. (who.int)
This cell then has therapeutic cloning: the global the capacity to divide and grow into an exact replica of the original from whom the debate somatic cell was taken. (who.int)

It has been shown that polyphenols isolated from red wine inhibit the growth of different cancer cells in vitro. (beauty104.com.tw)
Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
In this context, if it is possible to immortalize normal human SAE BC that retain the capacity to differentiate to ciliated, secretory, and other differentiated cell types in vitro on air-liquid interface (ALI) culture, it would be very useful to the investigation of SAE biology in health and disease, and the assessment of pharmacologic agents targeted to modify dysregulated BC biology relevant to the pathogenesis of human lung disease. (biomedcentral.com)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
Cell culture -Growth of cells in vitro on an artificial medium for experimental research. (cellmedicine.com)
Scientists showed that single-cell-cloned cancer stem cells, accidentally obtained from a human liver cancer microvascular endothelial cells, express classic stem cell markers, genes associated with self-renewal and pluripotent factors and possess colony-forming ability in vitro and the ability of serial transplantation in vivo . (stemcellsciencenews.com)
Gene therapy has been utilized successfully to repair or inactivate mutations in animal models of monogenic human diseases 19 as well as human in vitro cells 12 . (nature.com)
In vitro studies have shown DLKP-M to be extremely invasive relative to DLKP-SQ, whereas the latter cell line shows high anoikis resistance relative to DLKP-M. As the cells originate from the same tumour, these varied phenotypic characteristics make DLKP a particularly useful model for the study of cellular heterogeneity in lung cancer. (dcu.ie)
MARCKS and Desmoglein-3 were found to reduce cell motility in vitro upon transient protein knockdown using siRNA. (dcu.ie)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
We also evaluated the impact of proinflammatory molecules TNFα and IFNγ on IL32 expression and dedifferentiation in melanoma cell lines in vitro. (biomedcentral.com)
Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
In vitro models have been proposed in an attempt to better understand the early stages of osseointegration and how cells interact with surfaces modified by different methods. (bvsalud.org)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)

In 2001, Lorenz Studer, MD , Head of the Stem Cell and Tumor Biology Laboratory at MSKCC, and his colleagues at Rockefeller University published research in which they generated unlimited numbers of genetically matched dopamine nerve cells using cloned stem cells whose genetic material originated from the mouse's own tail. (mskcc.org)
This four-part small molecule cocktail, which we named CEPT (Chroman 1, Emricasan, trans-ISRIB, Polyamines), enhanced cell survival of genetically stable hPSCs by simultaneously blocking several stress mechanisms that otherwise compromise cell structure and function. (labroots.com)
Here we extend those findings to humans using only genetically unmodified human naive embryonic stem cells (cultured in human enhanced naive stem cell medium conditions) 4 . (nature.com)
Allogeneic-donor-derived cells can be genetically modified to eliminate expression of HLA-A. (ashpublications.org)
The lifespan of most cells is genetically determined, but some cell-culturing cells have been "transformed" into immortal cells which will reproduce indefinitely if the optimal conditions are provided. (wikipedia.org)
There already exist several protocols to genetically modify and differentiate iPSCs into NK cells, and each has its own advantages with regards to immunotherapies. (molcells.org)
It has been found that even within a group of genetically similar cells, small subgroups of resistant cells emerge. (cbirt.net)
They may instead be fusing with existing cells, creating genetically mixed-up tissues with unknown health effects" [1]. (i-sis.org.uk)
Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)

Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
GS-2 iPSCs showed the capacity to differentiate into cells of the hematopoietic lineage. (biomedcentral.com)

Pluripotent embryonic stem cells (ESCs) have the unique ability to differentiate into every cell type and to self-renew. (biomedcentral.com)
These adult stem cells are considered multipotent, having the ability to differentiate into different cell types, albeit with a more limited repertoire than embryonic stem cells. (thefutureofthings.com)
Embryonic stem cells are derived from early embryos and have the ability to differentiate into any cell type. (spiked-online.com)

CEPT improved routine cell passaging, cryopreservation/thawing of pluripotent and differentiated cells, embryoid body and organoid formation, single-cell cloning, genome editing, and new iPSC line generation. (labroots.com)
In pluripotent and differentiated cells HP1β is differentially localized and differentially associated with chromatin. (biomedcentral.com)

Cancer stem cells (CSCs) and poorly differentiated progenitor cells in pancreatic ductal adenocarcinoma (PDAC) exacerbate hierarchical tissue organization contributing to cell hyperproliferation and therapy resistance. (amegroups.org)
HMGCS2 protein expression is down-regulated preferentially in moderately and poorly differentiated carcinomas. (aacrjournals.org)
The DLKP cell line was established from a lymph node metastasis of a primary lung tumour and is described as a poorly differentiated squamous lung carcinoma cell line. (dcu.ie)

Much of this process relies on the morphogenesis of the extra-embryonic tissues and the effect this has on the organization of embryonic cells. (nature.com)
They are found mainly in renewing tissues, such as the skin, the inner lining of the gastrointestinal tract and blood tissues. (thefutureofthings.com)
In addition to their ability to supply cells at the turnover rate of their respective tissues, they can be stimulated to repair injured tissue caused by liver damage, skin abrasions and blood loss. (thefutureofthings.com)
The ability of our body to regenerate some of its tissues is largely owed to the reserves of adult stem cells. (thefutureofthings.com)
Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. (wikipedia.org)
Gottlieb Haberlandt first pointed out the possibilities of the culture of isolated tissues, plant tissue culture . (wikipedia.org)
[12] He suggested that the potentialities of individual cells via tissue culture as well as that the reciprocal influences of tissues on one another could be determined by this method. (wikipedia.org)
Astrocyte -One of the large neuroglia cells of neural tissues. (cellmedicine.com)
Cell-based therapies -treatment in which stem cells are induced to differentiate into the specific cell type required to repair damaged or depleted adult cell populations or tissues. (cellmedicine.com)
Blastocytes obtained through nuclear transfers would be used to generate the embryonic stem cells that could be differentiated to specific tissues or organs for transfer to the nuclear donor. (spiked-online.com)
Investigators examined human hepatocellular carcinomas in settings that selected for tumor formation from functionally defined tumor-initiating cells (TICs), and in which the origin of intratumoral endothelial cells from TICs as opposed to host tissues could be clearly distinguished. (stemcellsciencenews.com)
27 Jun, 2007 06:08 pm Stem cells have the potential to become all the cells and tissues in the human body. (scitizen.com)
embryonic stem cells that are isolated from the inner cell mass of blastocysts, and adult stem cells that are found in adult tissues. (techxplore.com)
In a developing embryo, stem cells can differentiate into all of the specialized embryonic tissues. (techxplore.com)
In adult organisms, stem cells and progenitor cells act as a repair system for the body, replenishing specialized cells, but also maintain the normal turnover of regenerative organs, such as blood, skin or intestinal tissues. (techxplore.com)
Stem cells can now be grown and transformed into specialized cells with characteristics consistent with cells of various tissues such as muscles or nerves through cell culture. (techxplore.com)
uniExM may find many uses in the simple, multimodal nanoscale analysis of cells and tissues. (plos.org)
Nanoscale imaging enables the analysis of biological systems, such as cells and tissues, at the fundamental length scales of biomolecules and biomolecular interactions, but classically has required expensive equipment and advanced skillsets to perform, e . g ., via super-resolution microscopy [ 1 ]. (plos.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)
The stem cells suits human needs, does not cause harm and can be obtained from both adult and fetal does not conflict with religious beliefs, it has tissues, umbilical cord and early embryos. (who.int)

An epithelial cell of the kidney. (ebisc.org)
By coupling the adhesion equipment towards the polarity proteins to modify mobile contractility, we suggest that MAGIs play important and central tasks in maintaining stable state intercellular pressure through the entire epithelial cell sheet. (aboutsciencenow.info)
Results Lack of ZO proteins highly perturbs Par-3 localization and alters apical morphology We previously demonstrated that depletion of ZO proteins in the mouse mammary epithelial cell range, EpH4, delays the forming of the contractile belt-like AJ18, recommending that ZO proteins are necessary for epithelial polarization. (aboutsciencenow.info)

Here, we show that the total polyphenol pool isolated from a red wine (varity Lemberger, vintage 1998) at micromolar concentrations inhibited the proliferation of transformed colon epithelial cells HT 29 clone 19A induced by epidermal growth factor (EGF). (beauty104.com.tw)
In contrast, activation of extracellular signal regulated kinase (ERK) 1/2 by EGF (1 nmol/L) was significantly inhibited by red wine polyphenols (6 mmol/L). This signaling pattern, activation of JNK 1/2 and p38 MAPK and inhibition of ERK 1/2, is typical for antiproliferative compounds, indicating that red wine polyphenols may inhibit the proliferation of colon carcinoma cells by modulating MAPK intracellular signal transduction pathways. (beauty104.com.tw)
In this study, total polyphenolic pool from red wine was isolated by solid-phase extraction and the effects of red wine polyphenols on proliferation and MAPK in human colon carcinoma cells were investigated. (beauty104.com.tw)
Mechanistically, EBF2 uniformly activates Pparγ-expression in pancreatic cancer cells and limits cell proliferation, whereas oncogenic drivers are expressed differentially in the progenitor cell subsets. (amegroups.org)
The complex PDAC pathobiology is established predominantly through KRAS mutations and the associated cellular signaling that contributes to cell proliferation and dedifferentiation ( 5 , 6 ). (amegroups.org)
It was also demonstrated that USF1 directly binds to the promoter region of 2,492 genes, which may be involved in the viral progression and cell proliferation pathways. (spandidos-publications.com)
Inocculate T75 cell culture flasks with 1x 10^4 cells/cm^2 (about 60.000 cells/ml, 12ml medium in one T75) for the proliferation process. (cls.shop)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
A subset of tumor cells, defined as cancer stem cells (CSCs), are characterized by self-renewal and continuous proliferation capacity, providing the ability to initiate tumor and to generate other heterogeneous cell populations [1]. (unicyte.ch)
Scientists showed that a 3D scaffold not only induced the diversification of cell morphologies but also increased the cell proliferation. (stemcellsciencenews.com)
Mesenchymal cells were isolated from periodontal ligament of impacted third molars (n=2) and cultured on titanium disks (polished and nitrided) and on a plastic surface as a positive control of cell proliferation. (bvsalud.org)
Cell proliferation was analyzed and growth curves were constructed for the different groups by determining the number of cells adhered to the different surfaces at 24, 48 and 72 h after plating. (bvsalud.org)
We concluded that plasma nitriding produced surfaces that permitted the proliferation of human periodontal ligament mesenchymal cells. (bvsalud.org)
In this respect, this study evaluated the proliferation of mesenchymal cells derived from the periodontal ligament of human third molars on polished and plasma-nitrided titanium surfaces. (bvsalud.org)

They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)
Unlike primary NK cells, those prepared from iPSCs can be prepared with a homogeneous quality and are easily modified to exert a desired response to tumor cells. (molcells.org)
Furthermore, T cells and NK cells complement each other in that certain immune suppression mechanisms taken by tumor cells that are effective against T cells, such as the downregulation of human leukocyte antigens (HLA), have proven to be stimulatory for NK cells. (molcells.org)
Moreover, EV-stimulated MSCs enhanced migration of renal tumor cells and induced vessel-like formation. (unicyte.ch)
In vivo, EV-stimulated MSCs supported tumor development and vascularization, when co-injected with renal tumor cells. (unicyte.ch)
In addition, tumor cells interaction were also shown to be mediated by secretion of extracellular vesicles (EVs) that can transfer proteins, lipids and nucleic acids and induce epigenetic changes in target cells [4,5]. (unicyte.ch)
Such dual role possibly depends on a complex mechanism of interaction between tumor cells and MSCs that can be initially triggered by tumor EVs. (unicyte.ch)
Current screening of potential new drugs is done using cell lines derived from animals or 'abnormal' human tissue such as tumor cells. (spiked-online.com)

Although it is possible to culture structures derived from human blastocysts ex vivo, these cultures do not recapitulate the events and structural organization of the in vivo embryos 6 ( Supplementary Information ). (nature.com)
The hybrid cells carrying four sets of chromosomes (instead of the usual two) behaved as stem cells when injected into mouse embryos. (i-sis.org.uk)
Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
Unicellular for those cells that are derived from human organisms are primed to replicate (clone) pre-embryos, which seem to have a high themselves by nature. (who.int)

Mouse naive embryonic stem cells have recently been shown to give rise to embryonic and extra-embryonic stem cells capable of self-assembling into post-gastrulation structured stem-cell-based embryo models with spatially organized morphogenesis (called SEMs) 3 . (nature.com)
Embryonic stem cells (ESCs), derived from the blastocyst-stage embryo, are capable of generating all cell types of the mammalian body (pluripotency) and of maintaining the capacity for indefinite self-renewal without compromising their genomic integrity. (biomedcentral.com)
The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
Blastocyst -A preimplantation embryo of about 150 cells. (cellmedicine.com)
Embryonic germ cells -Cells found in a specific part of the embryo/fetus called the gonadal ridge that normally develop into mature gametes. (cellmedicine.com)
Embryonic stem cells -Primitive (undifferentiated) cells from the embryo that have the potential to become a wide variety of specialized cell types. (cellmedicine.com)
These cells give rise to the embryonic disk of the later embryo and, ultimately, the fetus. (cellmedicine.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)

These ATG genes are highly conserved in mammalian cells, allowing to study their functions also in higher eukaryotes [ 1 - 4 ]. (hindawi.com)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
Compared to the large airway cell line BCi-NS1.1, differentiated of hSABCi-NS1.1 cells on ALI were enriched with small airway epithelial genes, including surfactant protein genes, LTF and small airway development relevant transcription factors NKX2-1, GATA6, SOX9, HOPX, ID2 and ETV5. (biomedcentral.com)
Promoter region motif analysis indicated that ETV5 has more binding motifs and genes than USF1 itself for USF1‑regulated DEGs, indicating that USF1 may indirectly modulate gene expression by regulating ETV5 expression in Huh7 cells. (spandidos-publications.com)
We found that CSC-derived EVs promoted persistent phenotypical changes in MSCs characterized by an increased expression of genes associated with cell migration (CXCR4, CXCR7), matrix remodeling (COL4A3), angiogenesis and tumor growth (IL-8, Osteopontin and Myeloperoxidase). (unicyte.ch)
Alternatively, transgenesis and gene targeting techniques can be used to introduce the patient's genes into the stem cell line. (spiked-online.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
Interestingly, P-Tex cells expressed CDK4 genes as high as cancer cells, which could be simultaneously inhibited by CDK4 inhibitors and might be a potential reason for the ineffectiveness of CDK4 inhibitors in treating HPV-positive HNSCC. (elifesciences.org)
But, although the mutation frequency of genes was much lower in ES cells, mutant ES cells accumulated with time in culture. (i-sis.org.uk)
In the tumor microenvironment, IL32 expression is highly correlated with genes related to T cell infiltration, and also positively correlates with high AXL/low MITF dedifferentiated gene signature. (biomedcentral.com)
The precise pathophysiology of inherited single cell and multilineage cytopenias has not been elucidated despite the identification of many of the genes mutated in these disorders. (medscape.com)

By experimenting on germ cells, cytologist Nettie Maria Stevens collected evidence to support the connection between heredity and the sex of offspring. (asu.edu)
This paper outlines the debates prompted through a reproduction mechanism involv- by progress in cloning research, with special ing male and female germ cells. (who.int)

Some in vivo transplantation studies have reported robust (35-50%) levels of transdifferentiation, which makes it unlikely that the results are due to cell fusion events. (i-sis.org.uk)

Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)

Conferring cell fitness by multi-target drug combinations may become a common approach in cryobiology, drug development, and regenerative medicine. (labroots.com)
In addition to retinal cells, other stem-cell derivatives are likely to play an important role in the future of regenerative medicine. (news-medical.net)
The researchers of the paper from Advanced Cell Technology, collaborated with scientists from the Institute of Regenerative Medicine at Wake Forest University School of Medicine, Winston-Salem, North Carolina, and the University of Chicago, Chicago, Illinios. (news-medical.net)
Advanced Cell Technology is a leading biotechnology company in the emerging field of regenerative medicine. (news-medical.net)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
The discovery of human pluripotent stem cells (PSCs) at the turn of the century opened the door to a new generation of regenerative medicine research. (molcells.org)

Although studies have identified unique transcription factors in the selection of normal pancreatic lineages, the cells of origin and molecular mechanisms arbitrating PDAC growth are poorly defined. (amegroups.org)
In addition, they do not require feeder layers and can differentiate into many neural lineages under the appropriate conditions (1). (ddw-online.com)
These unique clones allow for to study into specific epicardial cell lineages and phenotypes. (arizona.edu)

The ectopic expression of human papilloma virus (HPV) E6/E7 gene has successfully been employed to establish these cell lines. (lu.se)
The use of multiple hES-RPE lines in these studies will allow further correlation between function and gene expression. (news-medical.net)
Based on these findings, we propose that ketogenesis is an undesirable metabolic characteristic of the proliferating cell, which is down-regulated through c-Myc-mediated repression of the key metabolic gene HMGCS2 . (aacrjournals.org)
Griscelli syndrome type 2 (GS-2) is a rare, autosomal recessive immune deficiency syndrome caused by a mutation in the RAB27A gene, which results in the absence of a protein involved in vesicle trafficking and consequent loss of function of in particular cytotoxic T and NK cells. (biomedcentral.com)
All GS-2 iPSC clones displayed a normal karyotype (46XX or 46XY) and were shown to express the same RAB27A gene mutation that was present in the original somatic donor cells. (biomedcentral.com)
In this review, we examine the manufacturing and gene engineering of NK cells generated from iPSCs (iPS-NK) for ACT. (molcells.org)
In order to study the transcriptional regulation of IL32 in these cell lines, we cloned up to 10.5 kb of the 5′ upstream region of the human IL32 gene into a luciferase reporter vector. (biomedcentral.com)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
18 , 19 We surmised that cyclic cMOs containing an NfsB-cleavable linker could be used with analogous lines to knock down gene function in a tissue-specific manner (Figure 1 a). (cdc.gov)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)

Embryoid bodies -Clumps of cellular structures that arise when embryonic stem cells are cultured. (cellmedicine.com)

These include embryonic disc and bilaminar disc formation, epiblast lumenogenesis, polarized amniogenesis, anterior-posterior symmetry breaking, primordial germ-cell specification, polarized yolk sac with visceral and parietal endoderm formation, extra-embryonic mesoderm expansion that defines a chorionic cavity and a connecting stalk, and a trophoblast-surrounding compartment demonstrating syncytium and lacunae formation. (nature.com)
The ICM continues to differentiate into three germ layers-ectoderm, mesoderm and endoderm, each of which follows a specific developmental destiny that takes them along an ever-specifying path at which end the daughter cells will make up the different organs of the human body. (thefutureofthings.com)
Isolated clones were selected based on Epi5 vector loss, staining positive for pluripotency markers and ablility to differentiate into mesoderm, ectoderm and endoderm. (ebisc.org)

I fell in love with the idea of human pluripotent stem cells and started surveying the United States and even Europe for who was playing in that area. (medscape.com)

Using the lentiviral transfer of OSKM, we were able to generate different iPSC clones from 3 GS-2 patients. (biomedcentral.com)
Cellular Dynamics International (CDI) has continued to generate passage 5 iPSC lines from donor samples submitted to us by Tissue Collectors throughout the state of California. (ca.gov)

Stemness molecule expressions were determined in head and neck squamous cell carcinoma (HNSCC), as well as their properties and prognosis. (stemcellsciencenews.com)
The survival prognosis of human papillomavirus (HPV)-positive and HPV-negative head and neck squamous cell carcinoma (HNSCC) is largely different, and little is known about the anti-tumor mechanism of tumor-infiltrated exhausted CD8 + T cells (Tex) in HNSCC. (elifesciences.org)

F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
Several growth factors have been identified that promote growth of human ES cells in culture, most notably basic fibroblast growth factor (bFGF). (ddw-online.com)
A subset of epicardial cells undergoes EMT, migrates into the developing heart, and differentiates into cardiac fibroblast, vascular endothelial, and smooth muscle cells. (arizona.edu)

Ex vivo manufactured red blood cells (RBC) generated from immortalized erythroid cell lines which can continuously grow are expected to become a significant alternative in future transfusion therapies. (lu.se)
This is the first application of transcriptomics to assess ES cell derivatives and their in vivo counterparts -- a 'differentiomics' outlook. (news-medical.net)
These results show that IL-8 induces autocrine signalling via an apical CXCR1 in Caco-2 BBE intestinal epithelial cells and that this receptor is also expressed on the apical surface of differentiated human intestinal epithelial cells in vivo , suggesting an autocrine function for IL-8 secreted in the lumen. (biomedcentral.com)
They found that the spontaneous mutation frequency in ES cells is 100-fold lower than that in mouse embryonic fibroblasts (a somatic cell line), which is similar to adult cells in vivo . (i-sis.org.uk)

This resulted in a cohort of significantly differentially expressed proteins between the cell lines. (dcu.ie)
Right here, we propose a molecular system where AJC scaffolding proteins control apical cell contractility by differentially recruiting MAGI-1 and MAGI-3 to apical AZD-3965 junctions. (aboutsciencenow.info)

Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)

Finally, we note that the undifferentiated ESC nucleus itself shows less spatial organization than in differentiated cells. (biomedcentral.com)
DNA -Deoxyribonucleic acid, a chemical found primarily in the nucleus of cells. (cellmedicine.com)
When the nucleus of a stem cell has been the technique of cloning. (who.int)
The con- is removed and replaced by a nucleus of cept of human cloning has long been in the another cell type, the stem cell will then imagination of many scientists, scholars and be reprogrammed to produce the product fiction writers [ 1 ]. (who.int)
The basic techniques of of the implanted nucleus, when it fully cloning have been known for some time, and develops. (who.int)

ELLU is heterogeneous and, unexpectedly, clones expressing adult hemoglobin rapidly differentiate and produce fragile cells. (lu.se)
These clones provided direct evidence that the epicardium is a heterogeneous population of cells. (arizona.edu)
Shootin-1 and Desmoglein-3 expression were affected by co-culture of the clonal subpopulations, highlighting the heterogeneous nature of the original DLKP cell line and the influences this has on protein expression. (dcu.ie)

Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

To facilitate the study of the biology of the human SAE in health and disease, we immortalized and characterized a normal human SAE basal cell line. (biomedcentral.com)
Since Haberlandt's original assertions, methods for tissue and cell culture have been realized, leading to significant discoveries in biology and medicine. (wikipedia.org)
Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. (deepdyve.com)
One of the greatest controversies triggered tissue, a stem cell encoding for heart tissue by the rapid pace of evolution in biology, will eventually develop into heart tissue particularly in genomics and biotechnology, and so on. (who.int)

Highly plastic adult stem cells from a variety of sources, including umbilical cord blood and bone marrow, are routinely used in medical therapies. (techxplore.com)

Cells proliferate at 33 degree Celsius, and they differentiate at 37 degree Celsius. (cls.shop)

The tumor is composed by different cell populations. (unicyte.ch)
Kelly, Shane (2016) Proteomic characterisation of clonal populations from a humanlung carcinoma cell line. (dcu.ie)
FateMap helps identify resistant cells from subpopulations derived from single-cell (clonal) cancer populations and analyze their unique characteristics. (cbirt.net)
We envisioned that enzyme-activatable cMOs could overcome these limitations, as the triggering enzymes could be expressed in individual cell populations using cis regulatory elements. (cdc.gov)
Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

Adult stem cells can be used to accelerate bone or tendon healing , and they can induce cartilage progenitor cells to produce a better matrix and repair cartilage damage . (thefutureofthings.com)
Platelets are released from megakaryocytes (MKs) in the bone marrow and circulate as anucleate blood cells with a diameter of about 2-4 μm. (biomedcentral.com)
Bone marrow stromal cells -A stem cell found in bone marrow that generates bone, cartilage, fat, and fibrous connective tissue. (cellmedicine.com)
Bone marrow-derived mesenchymal stromal cells (MSCs) are known by their migratory capacity to injury and tumor sites. (unicyte.ch)
molecules secreted by the tumor and tumor-related stromal cells, promoting their recruitment from bone marrow into the circulation and subsequent engraftment within tumor microenvironment [14]. (unicyte.ch)
In his original report, Thompson demonstrated that human embryonic stem cells could be coaxed into developing gut-like structures, bone, cartilage and muscle (1). (spiked-online.com)
For example, it can cause bone marrow not to produce enough red blood cells, which can lead to anemia. (upscpathshala.com)
In the second report [3], mouse bone marrow cells marked with green fluorescent protein were found to fuse with ES cells in culture in the presence of the cytokine, interleukin-3, which is known to encourage cell fusion. (i-sis.org.uk)
These researchers pointed out that the spontaneous fusion rate (without interleukin-3) was extremely low, between 2-11 per million bone marrow cells, and is unlikely to account for all the findings with adult stem cells. (i-sis.org.uk)
The cells could be made to differentiate into bone forming cells, cartilage forming cells, fat cells, skeletal muscle cells and endothelial cells. (i-sis.org.uk)
The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)
Inherited bone marrow failure syndromes (IBMFS) are usually the result of intrinsic stem cell/progenitor defects. (medscape.com)

Mesenchymal stem cells -Cells from the immature embryonic connective tissue. (cellmedicine.com)
A number of celltypes come from mesenchymal stem cells, includingchondrocytes, which produce cartilage. (cellmedicine.com)

Accordingly, immunohistochemistry with specific antibodies shows that HMGCS2 is expressed mainly in differentiated cells of human colonic epithelium. (aacrjournals.org)
The HT-29-C1.19A cells were derived from the human colonic cancer cell line HT29. (mbpinc.net)
1984) Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. (mbpinc.net)
Interestingly, IL-8 has been measured in the colon milieu of healthy volunteers using a dialysis bag inserted in the rectum [ 9 ] suggesting that it might be apically secreted by colonic epithelial cells. (biomedcentral.com)

At present, the cells have been cultured successfully for more than 40 passages without noting phenotypic changes. (cls.shop)
It relieved the inhibitory effect of DNMT3A mutation, promoted the phenotypic recovery of the co-cultured macrophages, eliminated resistance, and regulated the immune microenvironment. (frontiersin.org)

Here, the stem cell line is created using the genetic properties of the prospective recipient via somatic cell nuclear transfer. (spiked-online.com)
Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)

Extracellular vesicles derived from renal cancer stem cells induce a pro-tumorigenic phenotype in mesenchymal stromal cells. (unicyte.ch)
CSCs were shown to secrete large amount of extracellular vesicles (EVs) that can transfer several molecules (proteins, lipids and nucleic acids) and induce epigenetic changes in target cells. (unicyte.ch)

Basal macroautophagy (thereafter referred to as autophagy), a catabolic recycling system in cells, is key to maintaining cellular homeostasis and survival. (hindawi.com)
In particular, Cellular Dynamics International (CDI) has taken lease to approximately 5000 square feet of lab space at the Buck Institute for Research on Aging in Novato, CA. The majority of this space is located within the new CIRM-funded Stem Cell Research Building at the Buck Institute and was extensively reconfigured to meet the specific needs of this grant. (ca.gov)
Stem cells are cells found in most, if not all, multi-cellular organisms. (techxplore.com)
The use of these cultures provides cellular and molecular data that favor a nanostructural engineering approach in implant design and permits the testing of different hypotheses. (bvsalud.org)

For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
Scientists anticipate that in the future stem cell lines will provide a virtually unending supply of pancreatic cells for diabetic patients, neuronal cells for patients with neural disorders such as Parkinson's or Alzheimer's disease, and a host of heart cells that may treat a variety of cardiac problems. (spiked-online.com)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

Furthermore, activation of autophagy allows to extend cell survival when exposed to different types of stressors such as starvation or cytotoxic drugs. (hindawi.com)
Human embryonic stem cells are so exciting because they provide a pathway of making many desperately-needed cell types for use in medicine," said Michael D. West, President & C.E.O. at ACT and an author on the paper. (news-medical.net)
At the top of the list comes the zygote-a fertilized egg, which of course has the ability to divide and differentiate into all cell types in the body and create a new organism. (thefutureofthings.com)
Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
In practice, the term "cell culture" now refers to the culturing of cells derived from multicellular eukaryotes , especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture , fungal culture, and microbiological culture (of microbes ). (wikipedia.org)
Among all cell types, however, stem cells in many ways have the best advantages. (molcells.org)
Adult stem cell -An undifferentiated cell found in a differentiated tissue that can renew itself and (with certain limitations) differentiate to yield all the specialized cell types of the tissue from which it originated. (cellmedicine.com)
Anti- tumoral effects have been reported in different types of tumors, showing a reduction in tumor growth and metastasis with the presence of MSCs or EVs derived from these cells [15-18]. (unicyte.ch)
In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
Two reports appeared as advance online publications in the top British journal Nature , accompanied by a news report that begins, "The hyped ability of adult stem cells to sprout replacement tissue types is being called into question. (i-sis.org.uk)
Neither report cited a paper published last year in the journal Blood [4], where a group from the Stem Cell Institute, Department of Medicine, and Cancer Center, University of Minnesota Medical School, Minneapolis, reported the most comprehensive experiments proving that a single adult stem cell can differentiate into all cell types in culture. (i-sis.org.uk)
They are characterized by the ability to renew themselves through mitotic cell division and differentiating into a diverse range of specialized cell types. (techxplore.com)
16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)
Homologous elements (composed of cell types normally found in the uterus) are typically undifferentiated spindle cell sarcoma, pleomorphic sarcoma, or fibrosarcoma. (medscape.com)

In human SH-SY5Y cells, the luciferase assay implied that Sirt2 was likely a target of miRNA-339. (hindawi.com)
Investigators evaluated the effect of the histone deacetylase inhibitors compound abexinostat on cancer stem cells from 16 breast cancer cell lines using ALDEFLUOR assay and tumorsphere formation. (stemcellsciencenews.com)

Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. (wikipedia.org)
The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows . (wikipedia.org)
The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture . (wikipedia.org)
[6] In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle of tissue culture. (wikipedia.org)
All equipment, including tissue culture safety cabinets and incubators, liquid-handling robotics, and QC instrumentation have been installed and qualified. (ca.gov)

Long-term engraftment of allogeneic cells necessitates eluding immune-mediated rejection, which is currently achieved by matching for human leukocyte antigen (HLA) expression, immunosuppression, and/or delivery of donor-derived cells to sanctuary sites. (ashpublications.org)
Small airway basal cells were purified from brushed SAE of a healthy nonsmoker donor with a characteristic normal SAE transcriptome. (biomedcentral.com)
By the end of year three, we have generated iPSC clones from 1737 donor samples (out of 2670 received). (ca.gov)
A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)
Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
Most of these efforts are focused on donor cells. (biomedcentral.com)

The first three divisions of the zygote give birth to eight totipotent cells, each of which also has the ability to become an entire organism. (thefutureofthings.com)

Accelerated hematopoietic cell apoptosis has been demonstrated in virtually all IBMFS. (medscape.com)

Lentivirus-mediated expression of SPDEF in hSABCi-NS1.1 cells induced secretory cell metaplasia, accompanied with characteristic COPD-associated SAE secretory cell changes, including up-regulation of MSMB, CEACAM5 and down-regulation of LTF. (biomedcentral.com)
After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
Overexpression of miR-339 downregulated Sirt2 expression, while knockdown of miR-339 upregulated Sirt2 expression in human SH-SY5Y cells and rat PC12 cells. (hindawi.com)
In addition, the expression of cancer stem cell markers of Sox2 and Oct4 were higher than that in 2D cultured cells. (stemcellsciencenews.com)
The expression patterns for these proteins were validated, and functional cell-based assays were carried out to establish the role of each protein in their respective clone. (dcu.ie)
Expression profiles for individual cells are then produced with scRNA-seq. (cbirt.net)
A significant proportion of established human melanoma cell lines express IL32, with its expression being highly correlated with a dedifferentiation genetic signature (high AXL/low MITF). (biomedcentral.com)
In the short-term AN model, iPS-MSCs conferred a substantial anti-apoptotic effect on tubular cells, associated with a downregulation of Bax and Bax/Bcl2 ratio and an upregulation of survivin expression. (oncotarget.com)

The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century. (wikipedia.org)
Banking of multiple cell lines with varying genetic spectrum that can be matched to patients is one possibility. (spiked-online.com)
Interestingly, no genetic foundation for cell diversity was observed within resistant clones. (cbirt.net)

To overcome this issue, we established immortalized megakaryocyte cell lines (imMKCLs) by introducing three transgenes, c-MYC, BMI1, and BCL-XL, sequentially into hematopoietic and megakaryocytic progenitor stage cells derived from iPSCs. (biomedcentral.com)
Hematopoietic stem cell -A stem cell from which all red and white blood cells develop. (cellmedicine.com)
Hematopoietic cells are almost totally absent. (medscape.com)
The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)

Through simply changing cell culture conditions, a newly established cell line, Erythroid Line from Lund University (ELLU), is able to differentiate toward mature cells, including enucleated reticulocytes. (lu.se)

Neither the title of the paper, nor the abstract mentioned that in the experiment, five out of 25 rats receiving the transplant died with "teratoma-like tumors" in their brains, a well-known hazard of ES cells. (i-sis.org.uk)

By manipulating the media and culture conditions, different developmental fates were elicited. (i-sis.org.uk)
These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)

Renal carcinomas have been shown to contain a population of cancer stem cells (CSCs) that present self-renewing capacity and support tumor growth and metastasis. (unicyte.ch)

Adult stem cells can be used to replace damaged heart-muscle cells and are used in practice today . (thefutureofthings.com)
The most common application of adult stem cells is probably the restoration of blood cells for patients with leukemia, and there are many more applications currently in practice. (thefutureofthings.com)
Adult Stem Cells: The New Plastic Surgery? (scitizen.com)
27 Jun, 2008 05:23 pm A new article in Live Science predicts that adult stem cells may replace silicone breast implants and other contemporary plastic surgery techniques. (scitizen.com)
In the latest skirmish, two rapid online publications are presented as if they were evidence against the existence of adult stem cells. (i-sis.org.uk)
This leaves one with the distinct impression that adult stem cells might not exist, and furthermore, they may be a health hazard. (i-sis.org.uk)
In fact, the senior authors in both reports admit that some adult stem cells may genuinely revert to an earlier stage of development or switch fates [1]. (i-sis.org.uk)
The finding that adult cells can fuse with ES cells is therefore irrelevant to the debate, and the fact that it was used in an attempt to discredit adult stem cells is itself revealing, particularly in view of other recent findings on ES cells. (i-sis.org.uk)
This latest anti-publicity on adult stem cells comes on the heels of a paper announcing success in embryonic stem (ES) cell transplant in a Parkinson rat model published in the house journal of the United States National Academy of Sciences [5]. (i-sis.org.uk)

The minor fraction of HP1β that is chromatin-bound in ESCs is enriched within exons, unlike the situation in differentiated cells, where it binds heterochromatic satellite repeats and chromocenters. (biomedcentral.com)
These cells have been described as embryonic stem cells (ESCs) and. (scitizen.com)

Anatomy13

Organisms5

Diseases2

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment17

Phenomena and Processes30

Information Science3

Induced plur12

Differentiation56

Therapeutic21

Vitro15

Genetically9

Capacity to differentiate2

Ability to differentiate3

Pluripotent and differentiated cells2

Poorly differentiated3

Tissues21

Epithelial cell3

Proliferation14

Tumor Cells8

Embryos4

Embryo9

Genes11

Germ cells2

Transplantation1

Progenitor cell1

Regenerative Medicine6

Lineages3

Gene10

Embryoid1

Mesoderm3

Human pluripotent stem1

Generate2

Squamous2

Fibroblast4

Vivo4

Differentially2

Mammalian1

Nucleus5

Heterogeneous3

Fetal2

Biology4

Therapies1

Proliferate1

Populations5

Bone12

Mesenchymal stem2

Colonic4

Phenotypic2

Somatic cell2

Induce2

Cellular4

Neural3

Types15

Assay2

Tissue culture5

Donor6

Totipotent1

Apoptosis1

Expression9

Genetic3

Hematopoietic4

Lund University1

Abstract1

Developmental3

Carcinomas1

Adult stem9

ESCs2