• enzymology
  • In enzymology, a carboxymethyloxysuccinate lyase (EC 4.2.99.12) is an enzyme that catalyzes the chemical reaction carboxymethyloxysuccinate ⇌ {\displaystyle \rightleftharpoons } fumarate + glycolate Hence, this enzyme has one substrate, carboxymethyloxysuccinate, and two products, fumarate and glycolate. (wikipedia.org)
  • In enzymology, a dihydroxyphenylalanine ammonia-lyase (EC 4.3.1.11) is an enzyme that catalyzes the chemical reaction 3,4-dihydroxy-L-phenylalanine ⇌ {\displaystyle \rightleftharpoons } trans-caffeate + NH3 Hence, this enzyme has one substrate, 3,4-dihydroxy-L-phenylalanine (L-DOPA), and two products, trans-caffeate and NH3. (wikipedia.org)
  • In enzymology, an ethanolamine ammonia-lyase (EC 4.3.1.7) is an enzyme that catalyzes the chemical reaction ethanolamine ⇌ {\displaystyle \rightleftharpoons } acetaldehyde + NH3 Hence, this enzyme has one substrate, ethanolamine, and two products, acetaldehyde and NH3. (wikipedia.org)
  • In enzymology, a threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.16) is an enzyme that catalyzes the chemical reaction threo-3-hydroxy-L-aspartate ⇌ {\displaystyle \rightleftharpoons } oxaloacetate + NH3 Hence, this enzyme has one substrate, threo-3-hydroxy-L-aspartate, and two products, oxaloacetate and NH3. (wikipedia.org)
  • In enzymology, a 3-ketovalidoxylamine C-N-lyase (EC 4.3.3.1) is an enzyme that catalyzes the chemical reaction 4-nitrophenyl-3-ketovalidamine ⇌ {\displaystyle \rightleftharpoons } 4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxycyclohex-2-en-1-one Hence, this enzyme has one substrate, 4-nitrophenyl-3-ketovalidamine, and two products, 4-nitroaniline and 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxycyclohex-2-en-1-one. (wikipedia.org)
  • In enzymology, a serine-sulfate ammonia-lyase (EC 4.3.1.10) is an enzyme that catalyzes the chemical reaction L-serine O-sulfate + H2O ⇌ {\displaystyle \rightleftharpoons } pyruvate + NH3 + sulfate Thus, the two substrates of this enzyme are L-serine O-sulfate and H2O, whereas its 3 products are pyruvate, NH3, and sulfate. (wikipedia.org)
  • enzymes
  • AP lyase enzymes catalyze reactions analogous to β-elimination reaction. (wikipedia.org)
  • AP lyase enzymes could be trapped on both pre-incised and unincised AP DNA by a reducing agent such as sodium borohydride. (wikipedia.org)
  • ABSTRACT: Members of the marine phytoplankton genus Phaeocystis (Prymnesiophyceae) produce large amounts of the intracellular osmolyte DMSP and they are known to also produce lyase enzymes that cleave DMSP into the biogeochemically important trace gas DMS. (int-res.com)
  • endonuclease
  • Initially, AP hydrolyses, such as apurinic or apyrimidinic endonuclease I contain a Mg2+ active site that cleaves the DNA on the 5'-side, yielding a 5'-deoxyribosephosphate and 3'-OH. (wikipedia.org)
  • In E. coli, DNA AP lyase (endonuclease III) helps repair oxidative damage to DNA bases by catalyzing the excision of the damaged pyrimidines and purines from ring saturation or opening from the DNA backbone. (wikipedia.org)
  • Bacteriophage T4 and Micrococcus luteus UV endonucleases were actually shown not to be under the class of "endonuclease," but rather were β-elimination catalysts for reactions at AP sites at the C3'-O-P bond-thus, classifying them as AP lyases. (wikipedia.org)
  • class
  • Since DNA AP lyase is a class of structures who have numerous target genes that encode for different variations of the enzyme, there is no one single enzyme structure that can be used as an example that encompasses all versions of the enzyme. (wikipedia.org)
  • Analysis
  • First-order kinetic analysis of DMSP lyase thermal denaturation demonstrated that the activation energy, free energy, enthalpy and entropy of denaturation in P. antarctica extracts were lower than for P. globosa extracts, confirming that the P. antarctica DMSP lyase was more thermolabile than the lyase from the temperate strain. (int-res.com)