• proteins
  • Within the FYVE domain there are Zinc finger binding sites that interact with phosphatidylinositol-3-phosphate, to bring target proteins to membrane lipids. (wikipedia.org)
  • PRP36 is 1346 amino acids long and is proline rich, meaning that a greater proportion of proline residues exist throughout the protein, including the DUF4596 domain, in comparison with other human proteins. (wikipedia.org)
  • Using human proteins, an affinity chromatography ran on TMEM33 showed that the protein bound to reticulon 4C, 1A, 2B, 3C, and Arl6IP1 in vitro. (wikipedia.org)
  • locus
  • In oligogenic analyses, the 2-locus LOD score (for chromosomes 3 and 4) increased significantly ( P =0.0012) to 6.1, but including the third locus on chromosome 6 did not significantly improve the LOD score ( P =0.064). (ahajournals.org)
  • 17 18 The evidence for these linkages has not been strong, with only the linkage of the dichotomous trait QTL to the LDL receptor locus 14 having a log 10 odds in favor of linkage (LOD) score that exceeded 3, a nominal criterion for significant evidence of linkage. (ahajournals.org)
  • We found LOH at HIPK-2 gene locus in 37.5% of PTCs, 14.3% of FTCs and 18.2% of follicular adenomas.To causally link these data with Gal-3 upregulation, we performed in vitro experiments, using the PTC-derived K1 cells, in which HIPK2 expression was manipulated by RNA interference (RNAi) or plasmid-mediated overexpression.HIPK2 RNAi was associated with Gal-3 upregulation, while HIPK2 overexpression with Gal-3 downregulation. (nih.gov)
  • Polymorphic DAZ gene family in polymorphic structure of AZFc locus: Artwork or functional for human spermatogenesis? (wikipedia.org)
  • Humans
  • Social interactions between humans have established an extremely wide variety of values, social norms, and rituals, which together form the basis of human society. (wikipedia.org)
  • Mapping
  • Cells were examined for cytopathology, evaluated by fluorescence in-situ hybridization (FISH) for the centromere of chromosome 3, analyzed by 250K whole genome Mapping Array and U133 plus 2.0 Expression Array, and placed in cell culture. (nih.gov)
  • At passage 3, the cell lines were analyzed by Mapping Array and Expression Array. (nih.gov)
  • Mapping involves (1) dividing the chromosomes into smaller fragments that can be propagated and characterized and (2) ordering (mapping) them to correspond to their respective locations on the chromosomes. (lsu.edu)
  • chimpanzee
  • The genus Homo evolved and diverged from other hominins in Africa, after the human clade split from the chimpanzee lineage of the hominids (great apes) branch of the primates. (wikipedia.org)
  • tumors
  • Acquired UPD is quite common in both hematologic and solid tumors, and is reported to constitute 20 to 80% of the LOH seen in human tumors. (wikipedia.org)
  • analyses
  • B) Inverted images of agarose gels showing semiquantitative RT-PCR analyses of HIPK2 and Gal-3 gene expression performed on total RNA extracted from K1 cells described in (A). (C) Western blot analysis of HIPK2 and Gal-3 in K1 cell transiently transfected with control vector pCMV-FLAG (Flag) and with pCMV-FLAG-HIPK2 (Flag-HIPK2) expression construct. (nih.gov)
  • D) Inverted images of agarose gels showing semiquantitative RT-PCR analyses of HIPK2 and Gal-3 gene expression performed on total RNA extracted from K1 cells described in (C). (E) Western blot analysis of HIPK2, p53Ser46, total p53 and Gal-3 in K1 cell transiently transfected with the kinase-dead pEGFP-HIPK2K221R (GFP-HIPK2K221R) and the wild type pEGFP-HIPK2 (GFP-HIPK2) HIPK2 expression constructs. (nih.gov)
  • serine
  • Human TMEM33 has phosphorylation predicted on serine residues 197 and 198 and threonine residues 5, 127, and 193. (wikipedia.org)
  • tumours
  • Our results indicate that HIPK2 expression and function are impaired in WDTCs, in particular in PTCs, and that this event explains Gal-3 overexpression typically observed in these types of tumours. (nih.gov)
  • Analysis
  • Here, we report new genotyping of eight SNPs showing some evidence of association in combined analysis of Stage 1 and Stage 2 in an additional 2048 cases of TGCT and 3944 controls (Stage 3). (forskningsdatabasen.dk)
  • Chromosome analysis was performed by standard 24 h-cultured method and R banding. (elsevier.com)
  • A) Western blot analysis, using antibodies to HIPK2 and Gal-3, performed on TCE obtained from PTC-derived K1 cell line stably transfected with pSUPER.retro control vectors (ctr) and pSUPER-HIPK2 interfering construct (HIPK2i). (nih.gov)
  • 1997
  • It was not until 1997 that human SARS and its enzyme product were isolated and expressed in Escherichia coli by a team from The European Molecular Biology Laboratory in France. (wikipedia.org)
  • amino acid
  • The mouse ortholog has been cloned and exhibits 63% homology to the human gene at the amino acid level. (wikipedia.org)
  • TMEM33 is predicted to have an O-glycosylation site at human amino acid residues 4 and 5 The exact secondary structure of TMEM33 is unknown, but it is predicted to be composed primarily of alpha-helices and coiled domains. (wikipedia.org)