AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
Radiation Hybrid MappingChromosome MappingHybrid CellsSequence Tagged SitesGenetic MarkersPhysical Chromosome MappingChromosomes, Human, Pair 1Genetic LinkageChromosomesMolecular Sequence DataExpressed Sequence TagsDNA, ComplementaryBase SequenceIn Situ Hybridization, FluorescenceAmino Acid SequenceRadiationCloning, MolecularZebrafishChromosomes, MammalianSequence Homology, Nucleic AcidSequence Homology, Amino AcidX ChromosomeChromosomes, HumanChromosome BandingRadiation DosageRadiation, IonizingChromosomes, Artificial, BacterialChromosome AberrationsChromosomes, PlantChromosomes, Human, Pair 17Chromosomes, Human, Pair 11Sex ChromosomesChromosomes, Human, Pair 5Radiation InjuriesChromosome BreakageChromosomes, Artificial, YeastChromosomes, BacterialChromosomes, Human, Pair 8Chromosomes, Human, Pair 19Polymerase Chain ReactionChromosome SegregationSequence Analysis, DNAChromosomes, Human, Pair 7Hybridization, GeneticDose-Response Relationship, RadiationBuffaloesMicrosatellite RepeatsChromosome DeletionChromosomes, Human, Pair 6GenomeRadiation ToleranceContig MappingChromosomes, Human, Pair 9Chromosomes, Human, Pair 21Chromosomes, Human, 6-12 and XChromosomes, FungalSyntenyCrosses, GeneticChromosomes, Human, Pair 2Chromosomes, Human, Pair 16Radiation ProtectionChromosomes, Human, Pair 22Chromosomes, Human, Pair 4Chromosome PairingChromosomes, Human, Pair 13Chromosomes, Human, Pair 10Radiation MonitoringKaryotypingChromosomes, Human, YChromosomes, Human, 1-3Chromosome DisordersTetraodontiformesChromosome PaintingGenome, HumanChromosomes, Human, Pair 12BupleurumGamma RaysChromosomes, Human, XLod ScoreChromosomes, Human, Pair 15Chromosomes, Human, Pair 14Chromosomes, Human, 16-18Models, GeneticChromosomes, Human, Pair 18Chromosomes, Human, Pair 20Cricetinae
HomologousGeneGenomicsSomatic cell hybridsGenes and chromosomesAnalysis of human chromosomeSequenceMouse chromosomeCytogeneticConservation of syntenyLocusHybridizationLociLinkageGenomicEuchromatic portionFelineMicrosatelliteHuman genomeInfertileSyntenicGeneticsChromosomalPredominantlyAssemblyLibrariesPolymorphic markersCandidate genesBipolarAssignmentGeneticallyRaudseppBreakpointsClonesBacterialContigSequencesMolecular markersDistinct1984RegionsOrganizationConstructGenomesFramework
Homologous7
  • A comparative radiation hybrid map of bovine chromosome 18 and homologous chromosomes in human and mice. (fbn-dummerstorf.de)
  • A radiation hybrid (RH)-derived physical map of 25 markers on the feline X chromosome (including 19 Type I coding loci and 6 Type II microsatellite markers) was compared to homologous marker order on the human and mouse X chromosome maps. (nova.edu)
  • River buffalo chromosome 1 (BBU1) is a sub-metacentric chromosome homologous to bovine chromosomes 1 and 27. (geneticsmr.com)
  • However, these maps will now be anchored to a new, different backbone and therefore, different homologous regions from different chromosomes may be loaded as well. (animalgenome.org)
  • Quantitative trait loci (QTL) for age of puberty, nipple number and ovulation rate have been identified in Meishan crosses on pig chromosome 10q (SSC10) near the telomere, which is homologous to human chromosome 10p15 and contains an aldo-keto reductase (AKR) gene cluster with at least six family members. (biomedcentral.com)
  • One of the QTL regions is located on the long arm of pig chromosome 10 (SSC10q) near the telomere, which is homologous to human chromosome 10p15 [ 5 , 6 ]. (biomedcentral.com)
  • The homologous region on mouse chromosome 13 contains a cluster of eight or nine AKR1C genes [ 12 ]. (biomedcentral.com)
Gene21
  • Constraints on the design of primers were to avoid presence of long introns, whose position and length was predicted on the basis of the orthologous human gene structure, and to design primers in the most divergent regions of the human/chicken alignment, to limit cross-amplification with the hamster DNA present in the hybrids. (biomedcentral.com)
  • Each cataract gene ortholog was mapped in relation to over 3,000 markers including microsatellites, ESTs, genes, and BAC clones. (upenn.edu)
  • The resulting independently determined RH-map locations were compared with the corresponding gene locations from the draft sequence of the canine genome. (upenn.edu)
  • All genes mapped within or near chromosomal locations with previously established homology to the corresponding human gene locations based on canine:human chromosomal synteny. (upenn.edu)
  • Assignment of the protein-tyrosine phosphatase beta gene (PTPRB) to cattle chromosome 5q23→q24 by in situ hybridization and somatic cell panel analysis. (fbn-dummerstorf.de)
  • Our long-term goal is to develop the necessary gene mapping tools for feline geneticists to identify and characterize genes causing disease in cats. (vin.com)
  • The current cat gene map contains over 1,900 markers mapped in a feline radiation hybrid (RH) panel. (vin.com)
  • This study will cytogenetically map 110 large-insert clones from the feline genome assembly to connect the cat gene maps to each feline chromosome. (vin.com)
  • Tight linkage and a conserved gene order for a segment encoding three genes, DFFRY-DBY-UTY in human, mouse, and cat Y chromosomes, coupled with demonstrated deletion effects of these genes on reproductive impairment in both human and mouse, implicates the region as critical for Y-mediated sperm production. (nova.edu)
  • A gene-based radiation hybrid map of chicken microchromosome 14: comparison to human and alignment to the assembled chicken sequence. (inra.fr)
  • Development of a gene-based radiation hybrid map of chicken Chromosome 7 and comparison to human and mouse. (inra.fr)
  • 1999) mapped the gene to chromosome 1p31. (nsjbio.com)
  • However, the challenges in genome annotation remain daunting [ 10 ], and the research community can anticipate years of additional work and manual curation to produce a true gene map of high quality. (biomedcentral.com)
  • Introduction Gene mapping represents the methods operated to specify a gene's location and spread between genes. (ganeshdiagnostic.com)
  • The spaces between various sites inside a gene can also be characterized through gene mapping. (ganeshdiagnostic.com)
  • Both classes of genome mapping compass scientists toward the site of a gene (or unit of DNA) on a chromosome. (ganeshdiagnostic.com)
  • A novel human myo-inositol monophosphatase gene, IMP.18p, maps to a susceptibility region for bipolar disorder. (rhea-db.org)
  • This might be because rearrangements in the gene-poor Y chromosome have milder effect on the phenotype, as a result of which the affected individuals escape attention and are not subjected to cytogenetic analysis. (veteriankey.com)
  • The probes have been used for molecular hybridizations (fluorescence in situ hybridization, or FISH) in clinical cytogenetics, Y chromosome mapping, sequencing, gene discovery, and comparative Y chromosome studies between the horse and other equids/ Perssodactyls. (veteriankey.com)
  • In the human, an aldo-keto reductase (AKR) gene family (AKR1C) has been identified near the telomere on chromosome 10p15 that contains at least six aldo-keto reductase family 1, member C genes [ 11 ]. (biomedcentral.com)
  • Overlapping of cloned or sequenced DNA to construct a continuous region of a gene, chromosome or genome. (umassmed.edu)
Genomics3
  • A heritable map (also named a linkage map) indicates the comparable background of genetic factors (reflecting on the sites of genomics variants) on a chromosome. (ganeshdiagnostic.com)
  • Irina began her career in academia at the Danish Academy of Sciences, Denmark researching mammalian genome mapping and at the University of Iowa Hospitals and Clinics, heading the cross-departmental functional genomics team focused on biomarker discovery to understand disease mechanisms and discover new drug targets. (sachibio.com)
  • An important breakthrough in horse Y chromosome research came with the availability of new genomics tools and resources, such as large-insert genomic bacterial artificial chromosome (BAC) libraries (for review, see Rubes et al. (veteriankey.com)
Somatic cell hybrids1
  • Genomic DNA from the somatic cell hybrids was tested for hybridization with each of an ordered set of probes used previously to construct a genetic map of chromosome 10, as well as several additional probes, previously localized by in situ hybridization. (ncsu.edu)
Genes and chromosomes1
  • This canine reference family DNA helped researchers place hundreds of markers near specific genes and chromosomes, improving studies of how diseases - such as familial cancers - develop in both dogs and people. (purinainstitute.com)
Analysis of human chromosome1
  • The DNA sequence and comparative analysis of human chromosome 20. (unige.ch)
Sequence15
  • To confirm the validity of the map and to provide valuable comparative mapping information, both markers from the genetic map and a high number of ESTs (Expressed Sequence Tags) were used. (biomedcentral.com)
  • Only two discrepancies were observed in relation to the sequence assembly recently reported for this chromosome. (biomedcentral.com)
  • This information was used to develop markers from chicken EST sequence data orthologous to genes in these human regions, in addition to the existing markers from the chicken chromosome 5 genetic map. (biomedcentral.com)
  • While in the process of finishing our map, the first draft sequence assembly of the chicken genome was released (March 1st, 2004). (biomedcentral.com)
  • The quality of both the GGA5 RH map and of the sequence assembly was therefore checked by alignment of all the markers by BLAST searches. (biomedcentral.com)
  • Francis Quetier: "I remember 2 young ladies named Catherine & Kellye who caught me in a meeting to get 2 M € from ANR to sequence the 3B chromosome. (tiki-toki.com)
  • Addition of the microchromosome GGA25 to the chicken genome sequence assembly through radiation hybrid and genetic mapping. (inra.fr)
  • Construction of a radiation hybrid map of chicken chromosome 2 and alignment to the chicken draft sequence. (inra.fr)
  • As of late 2000, the public human sequence was primarily based on approximately 24,000 accessioned bacterial artificial chromosome (BAC) clones covering 97% of the euchromatic portion of the genome [ 6 ]. (biomedcentral.com)
  • Thirty percent of the genome is in finished form, including the entire sequence of chromosomes 21 and 22 [ 7 ]. (biomedcentral.com)
  • Similarly, formerly independent maps, based on cytogenetic banding patterns, meiotic crossovers and radiation hybrids, may be placed within the single consensus sequence. (biomedcentral.com)
  • sequence mapping. (ganeshdiagnostic.com)
  • A backbone map is the primary map that will serve as the anchor off of which all comparative maps loaded will locate their sequence. (animalgenome.org)
  • Comparison of sequence data with the porcine BAC fingerprint map show that the cluster of genes resides in a 300 kb region. (biomedcentral.com)
  • Using the latest version of the draft sequence, the differentially expressed genes were mapped to the pig genome. (biomedcentral.com)
Mouse chromosome1
Cytogenetic3
  • The cytogenetic map will add an extra level of quality control to the feline genome maps, which will better enable feline researchers to identify the genetic bases of feline diseases and will enhance the health of the species. (vin.com)
  • Cytogenetic mapping takes up data received by microscopic examination of stained divisions of the chromosome. (ganeshdiagnostic.com)
  • Compared to autosomes and the X chromosome, relatively few numerical or structural cytogenetic abnormalities have been reported for ECAY. (veteriankey.com)
Conservation of synteny2
  • The available human/chicken comparative mapping data indicated conservation of synteny between GGA5 and portions of HSA11, HSA14 and HSA15. (biomedcentral.com)
  • Complete conservation of synteny and marker order was observed between feline and human X chromosomes, whereas the same markers identified a minimum of seven rearranged syntenic segments between mouse and cat/human X chromosome marker order. (nova.edu)
Locus3
  • Similarly, Y chromosome locus order was remarkably conserved between cat and human Y chromosomes, with only one marker ( SMCY ) position rearranged between the species. (nova.edu)
  • Here, we report the mapping of a novel ARNSHI locus, DFNB63, to chromosome 11q13.3-q13.4 in a large consanguineous Tunisian family. (shengsci.com)
  • DFNB63 represents the sixth ARNSHI locus mapped to chromosome 11. (shengsci.com)
Hybridization3
  • Hybridization of an unmapped probe to the cell line DNAs can be used to determine its most likely position on the chromosome relative to the mapped set of probes. (ncsu.edu)
  • Genomic DNA from two of the cell lines has been used to construct region-specific cosmid and bacteriophage libraries, and clones derived from these libraries were localized by hybridization to the panel of hybrid cell lines. (ncsu.edu)
  • Assignment of ANGPT4, ANGPT1, and ANGPT2 encoding angiopoietins 4, 1 and 2 to human chromosome bands 20p13, 8q22.3-->q23 and 8p23.1, respectively, by in situ hybridization and radiation hybrid mapping. (unige.ch)
Loci1
  • So far, 50 published autosomal recessive non-syndromic hearing impairment (ARNSHI) loci have been mapped, and 23 ARNSHI genes have been identified. (shengsci.com)
Linkage1
  • Linkage studies with X-chromosome markers have remained inconclusive, showing both positive and negative results. (shengsci.com)
Genomic4
  • You are able to load genomic, genetic or radiation hybrid maps. (animalgenome.org)
  • All comparitive maps are based on the UCSC synteny for genomic maps. (animalgenome.org)
  • In the current version of VCMap, if you wish to load genetic or radiation hybrid comparative map, the genomic version of that map must be loaded before the genetic or radiation hybrid comparative map. (animalgenome.org)
  • 2009), chromosome flow sorting and microdissection technology, and various genomic DNA amplification systems (Telenius et al. (veteriankey.com)
Euchromatic portion1
  • This polymorphism is primarily attributed to the various amount of Y heterochromatin, though earlier studies also suggest variation in the euchromatic portion of the chromosome (Power, 1988). (veteriankey.com)
Feline1
  • 6 Since then, Purina researchers collaborated with other scientists to create a higher resolution map of the feline chromosomes. (purinainstitute.com)
Microsatellite2
  • In this study, we constructed a new framework radiation hybrid (RH) map from BBU1 using BBURH 5000 panel adding nine new genes ( ADRB3 , ATP2C1 , COPB2 , CRYGS , P2RY1 , SLC5A3 , SLC20A2 , SST , and ZDHHC2 ) and one microsatellite (CSSM043) to the set of markers previously mapped on BBU1. (geneticsmr.com)
  • Using the IMpRH 7000rad and IMNpRH2 12000rad radiation hybrid panels, these 5 genes mapped between microsatellite markers SWR67 and SW2067 . (biomedcentral.com)
Human genome3
  • The high resolution framework map obtained in this study has markers covering the entire chicken chromosome 5 and reveals the existence of a high number of rearrangements when compared to the human genome. (biomedcentral.com)
  • Genome mapping furnished a crucial beginning point for the Human Genome Project. (ganeshdiagnostic.com)
  • The dashed arrows outline an alternative strategy where comparisons are made on the human genome, using comparative mapping approaches. (biomedcentral.com)
Infertile2
  • Additionally, infertile male patients present a number of breakpoints throughout chromosome 1. (geneticsmr.com)
  • Additionally, there are two reports about infertile stallions having XXY sex chromosomes - an equine counterpart to human Kleinfelter syndrome (Kubien et al. (veteriankey.com)
Syntenic1
  • When other comparative maps are loaded on top of that backbone, they will load all chromosomes that have syntenic regions that fall on the single chromosome of the backbone map, usually from more than one chromosome. (animalgenome.org)
Genetics1
  • By surveying patterns, the comparative order and location of genetics makers along a chromosome can be inaugurated. (ganeshdiagnostic.com)
Chromosomal2
  • The chromosomal location of Dp111 was determined by radiation hybrid mapping. (elsevierpure.com)
  • 1993. Protective effects of chlorogenic acid, curcumin and beta-carotene against gamma-radiation-induced in vivo chromosomal damage. (cdc.gov)
Predominantly1
  • Balanced reciprocal translocations associated with reproductive failures are predominantly observed on chromosome 1. (geneticsmr.com)
Assembly3
  • Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5. (biomedcentral.com)
  • We describe numerous global features of the genome and examine the relationship of various genetic maps with the assembly. (biomedcentral.com)
  • In this study, we report a chromosome-level genome assembly of the Muscovy duck, with a contig N50 of 11.8 Mb and scaffold N50 of 83.16 Mb. (bvsalud.org)
Libraries2
  • The panel of hybrid cell lines is thus useful for rapidly localizing unmapped probes and as a source of DNA for the construction of recombinant libraries derived from specific regions of the chromosome. (ncsu.edu)
  • Catherine to Jaroslav: "I believe we can do it with chromosome BAC libraries for 2003 sequencing! (tiki-toki.com)
Polymorphic markers1
  • Purpose: To facilitate the molecular characterization of naturally occurring cataracts in dogs by providing the radiation hybrid location of 21 cataract-associated genes along with their closely associated polymorphic markers. (upenn.edu)
Candidate genes2
  • QTL mapping and mining for functional candidate genes affecting health - the German ADR QTL Dairy Cattle Project. (fbn-dummerstorf.de)
  • Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2. (nih.gov)
Bipolar2
  • Mutational analysis of two positional candidate susceptibility genes for bipolar disorder on chromosome 12q23-q24: phenylalanine hydroxylase and human LIM-homeobox LHX5. (wikigenes.org)
  • Within the broad susceptibility region for bipolar disorder on the pericentromeric portion of chromosome 18, the highest allele sharing in our 22-pedigree series has been found in markers mapping to 18p11.2. (rhea-db.org)
Assignment2
  • Identification and chromosome assignment of 23 genes expressed in meat and dairy cattle. (fbn-dummerstorf.de)
  • Assignment of the human postmeiotic segregation increased (S. cerevisiae) 1 (PMS1) to chromosome 2q31.1 by radiation hybrid mapping. (nih.gov)
Genetically2
  • Several of these probes reveal restriction fragment length polymorphisms which have been genetically mapped. (ncsu.edu)
  • Since the long arm of ECAY carries the MSY and the PAR, the cells with i(Y) are genetically similar to those with Y chromosome disomy (YY). (veteriankey.com)
Raudsepp1
  • The only true Y chromosome structural rearrangements, reported so far, are large-scale ECAY deletions in 64,XY sex reversal mares (Raudsepp et al. (veteriankey.com)
Breakpoints1
  • Here, we report the breakpoints on chromosome 1 translocation and the clinical features presented in carriers, to enable informed genetic counseling of these patients. (geneticsmr.com)
Clones1
  • Screening the porcine CHORI-242 BAC library with a full-length AKR1C4 cDNA identified 7 positive clones and sample sequencing of 5 BAC clones revealed 5 distinct AKR1C genes ( AKR1CL2 and AKR1C1 through 4 ), which mapped to 126-128 cM on SSC10. (biomedcentral.com)
Bacterial1
  • The resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. (biomedcentral.com)
Contig5
  • Radiation hybrid maps have a resolution power intermediate to that of the genetic and BAC contig maps and are also a powerful tool for the mapping of ESTs and genes by simple PCR. (biomedcentral.com)
  • Contig Mapping" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • This graph shows the total number of publications written about "Contig Mapping" by people in this website by year, and whether "Contig Mapping" was a major or minor topic of these publications. (umassmed.edu)
  • Below are the most recent publications written about "Contig Mapping" by people in Profiles. (umassmed.edu)
  • A 12-Mb complete coverage BAC contig map in human chromosome 16p13.1-p11.2. (umassmed.edu)
Sequences1
Molecular markers1
Distinct1
  • Genetic mapping darts at how genetic knowledge is shuffled between chromosomes or between distinct parts of the identical chromosome during meiosis which is defined as a division of a cell into two). (ganeshdiagnostic.com)
19841
  • We report data on diagnoses made on amniotic fluid specimens from 1977 to 1984 as reported to the New York State Chromosome Registry. (shengsci.com)
Regions1
  • Therefore, microdissected Y-chromosome probes are good for FISH but not suitable for cloning and sequencing of the male-specific or pseudoautosomal regions of the chromosome. (veteriankey.com)
Organization2
  • Extensive Conservation of Sex Chromosome Organization Between Cat and " by William J. Murphy, Shan Sun et al. (nova.edu)
  • Integrative mapping analysis of chicken microchromosome 16 organization. (inra.fr)
Construct1
  • The chicken ChickRH6 panel recently produced was used here to construct a high resolution RH map of chicken GGA5. (biomedcentral.com)
Genomes1
  • The Plant Journal 39, 960-968 Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat. (tiki-toki.com)
Framework2
  • Moreover, once framework RH maps of a genome have been constructed, a quick location of markers by simple PCR on the RH panel is possible. (biomedcentral.com)
  • The final map is composed of 73 framework markers extending over a 1315.6 cR distance. (biomedcentral.com)