MSCsOsteoblastsMesenchymalHuman chondrocytesInduced in vitroCellsInvolved in OA pathogenesisExtracellular matrixApoptosisMarkersEarly chondrogenesisProliferationHypertrophyProliferativeDifferentiateOsteoarthriticHypertrophicProteinsCartilage matrixBoneMetabolismChondrogenicPhenotypeInflammationOxidative stressPathways involvedBovineCollagenDifferentiation potentialArticular cartilageAlterationsVivoRegulateChondralMineralizationInclusionsAberrantViabilityVesselCulturesCell
MSCs13
- MSCs undergoing chondrogenesis are preferentially responsive to an electromagnetic efficacy window defined by field amplitude, duration and frequency of exposure. (nature.com)
- MSCs can be differentiated along different cell lineages of mesodermal origin including osteoblasts, chondrocytes, skeletal myocytes or visceral stromal cells 9 . (nature.com)
- This correlated with enhanced chondrogenesis and a reduction in the expression of markers associated with chondrocyte hypertrophy, as well as increased SMAD 2/3 nuclear localization in the encapsulated MSCs. (amrita.edu)
- Mesenchymal stem cells (MSCs) are well known to have the capability to form bone and cartilage, and chondrogenesis derived from MSCs is reported to be affected by mechanical stimuli. (qigonginstitute.org)
- Mobilized pluripotent MSCs from the subchondral bone migrate into the defect filled with the clot, differentiate into chondrocytes and osteoblasts, and form a repair tissue over time. (hindawi.com)
- MSCs have multilineage differentiation potential, allowing them to differentiate, for example, into chondrocytes and osteoblasts, the key cells from the two tissues that constitute the osteochondral unit. (hindawi.com)
- In the orthopaedic field, additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation has been recently studied, since the bone marrow itself is both a source of MSCs, providing a cell population capable of chondrogenesis and of various growth factors stimulating cartilage repair [ 7 - 10 ]. (hindawi.com)
- Moreover, the bone marrow clot provides a three-dimensional (3D) environment which supports the chondrogenesis of MSCs. (hindawi.com)
- In contrast to the cost- and labor-intensive cultivation and propagation of cells such as MSCs or articular chondrocytes, the clinical use of "minimally processed" autologous BMA that can be prepared in the operation room as a single-step procedure appears straightforward. (hindawi.com)
- Introduction: Mesenchymal stem cells (MSCs) can be isolated from adult bone marrow (BM), expanded, and differentiated into several cell types, including chondrocytes. (elsevierpure.com)
- The aim of our study was to define the biological role of IGF-I on proliferation, chondrogenic condensation, apoptosis, and differentiation of MSCs into chondrocytes, alone or in combination with TGF-β and in the presence or absence of TGF-β signaling. (elsevierpure.com)
- Our data, by showing the role of IGF-I and TGF-β1 in the critical steps of MSC chondrogenesis, provide critical information to optimize the therapeutic use of MSCs in cartilage disorders. (elsevierpure.com)
- Description: Matrilins (MATNs) are a family of non-collagenous extra-cellular matrix (ECM) proteins consisting of four known members that have been proposed to play key roles in modulating cellular phenotypes during chondrogenesis of mesenchymal stem cells (MSCs). (ayurvedandindia.com)
Osteoblasts3
- Adipose tissue-derived stem cells (ADSCs) have been characterized as having the ability to self-renew and differentiate into different connective tissue cells, including osteoblasts, adipocytes, chondrocytes and myocytes, under specific inductive stimuli ( 4 ). (spandidos-publications.com)
- Skeletal tissues, bone, cartilage, muscle and tendon are mechano-responsive tissues consisted of osteoblasts, chondrocytes, myocytes and fibroblasts and their specific extracellular matrices (ECMs). (elsevierpure.com)
- This, in turn, precipitates chondrocyte apoptosis, thereby, osteoblasts invade the hypertrophic zone and bone formation occurs [ 5 ]. (pfmjournal.org)
Mesenchymal6
- Noggin, a developmental protein, inhibits chondrogenesis by preventing condensation and differentiation of mesenchymal cells. (wikipedia.org)
- Korpershoek JV, Rikkers M, Wallis FSA, Dijkstra K, Te Raa M, de Knijff P, Saris DBF , Vonk LA. Mitochondrial Transport from Mesenchymal Stromal Cells to Chondrocytes Increases DNA Content and Proteoglycan Deposition In Vitro in 3D Cultures. (mayo.edu)
- We discovered LNA043-a derivative of angiopoietin-like 3 (ANGPTL3)-as a potent chondrogenesis inducer using a phenotypic screen with human mesenchymal stem cells. (nih.gov)
- LNA043 exerts at least part of these effects through binding to the fibronectin receptor, integrin α 5 β 1 on mesenchymal stem cells and chondrocytes. (nih.gov)
- Kartogenin (KGN), a small molecular compound, can induce bone marrow-derived mesenchymal stem cells (BMSCs) into chondrocytes. (figshare.com)
- The previous in vitro study showed that kartogenin also had a chondrogenesis effect on synovium derived mesenchymal stem cells (SMSCs). (figshare.com)
Human chondrocytes3
- Are biological agents toxic to human chondrocytes and osteocytes? (biomedcentral.com)
- Mechanical stimuli have been shown to enhance chondrogenesis on both animal and human chondrocytes cultured in vitro. (polimi.it)
- The results showed that Fer could effectively reverse the erastin-induced inhibition of human chondrocyte viability, increase the level of collagenous proteins in human chondrocytes, and inhibit oxidative stress and ferroptosis. (biomedcentral.com)
Induced in vitro1
- In addition, inflammation‑inflicted oxidative stress was induced in vitro by stimulating chondrocytes with H2O2. (spandidos-publications.com)
Cells6
- Bone morphogenetic proteins are growth factors released during embryonic development to induce condensation and determination of cells, during chondrogenesis. (wikipedia.org)
- In the present study, the potential of using exosomes isolated from adipose‑derived stem cells (ADSCs) as a therapeutic tool for reducing chronic inflammation and promoting chondrogenesis was investigated using patient‑derived primary cells. (spandidos-publications.com)
- In vitro expansion of adipose-derived adult stromal cells in hypoxia enhances early chondrogenesis. (ox.ac.uk)
- Using a defined in vitro model of early chondrogenesis, we attempted to enrich for cells with an enhanced ability for chondrogenic differentiation by pre-exposure of mouse adipose-derived adult stromal cells (ADASs) to a hypoxic (2% oxygen) environment. (ox.ac.uk)
- The resting zone, a source of stemlike progenitor cells that restores the reservoir of proliferative chondrocytes, is located farthest from the primary ossification center and assumes responsibility for preserving the architectural integrity of the growth plate [ 4 ]. (pfmjournal.org)
- Human hyaline cartilage is comprised of a unique profile of chondrocytes (cells), matrix proteins (signals), and tissue architecture (scaffold), which together this advanced structure-function relationship imparts critical properties to the joint spaces such as cushioning and lubrication [ 5 ]. (biomedcentral.com)
Involved in OA pathogenesis1
- We performed a quantitative proteomic analysis in osteoarthritic and normal chondrocytes followed by functional analyses to investigate proteome changes and molecular pathways involved in OA pathogenesis. (biomedcentral.com)
Extracellular matrix3
- Chondrification (also known as chondrogenesis) is the process by which cartilage is formed from condensed mesenchyme tissue, which differentiates into chondrocytes and begins secreting the molecules that form the extracellular matrix. (wikipedia.org)
- The proliferative zone, formed by cellular division originating from the resting zone, features flattened chondrocytes that undergo rapid replication, arrange longitudinally, and synthesize substantial amounts of extracellular matrix. (pfmjournal.org)
- Bovine chondrocytes proliferated and produced a type II collagen and a sulfated glycosaminoglycan-rich extracellular matrix on all scaffolds. (ox.ac.uk)
Apoptosis2
- Treatment with exosomes protected articular chondrocytes from H2O2‑induced apoptosis. (spandidos-publications.com)
- Results and Conclusions: IGF-I modulated MSC chondrogenesis by stimulating proliferation, regulating cell apoptosis, and inducing expression of chondrocyte markers. (elsevierpure.com)
Markers4
- However, this was accompanied by increased markers of chondrogenesis. (qigonginstitute.org)
- The protein expression of chondrocyte-specific markers of Aggrecan, Sox9, and BMP7 were upregulated and Collagen X was decreased by LMHF vibration introduced at the chondrogenic differentiation in vitro. (qigonginstitute.org)
- This study provides evidence for further testing on the molecular mechanism of the disease and also propose proteins as candidate markers of OA chondrocyte phenotype. (biomedcentral.com)
- The protein expression of the human chondrocyte markers Aggrecan and collagen II was identified by immunofluorescence, according to Li et al. (biomedcentral.com)
Early chondrogenesis2
- This study highlights the intricacies of calcium homeostasis during early chondrogenesis and the constraints that are placed on PEMF-based therapeutic strategies aimed at promoting MSC chondrogenesis. (nature.com)
- Furthermore, re-oxygenation of the 2% oxygen-expanded ADASs before differentiation did not significantly affect early chondrogenesis. (ox.ac.uk)
Proliferation4
- Ihh stimulates chondrocyte proliferation and regulates chondrocyte maturation by maintaining the expression of PTHrP. (wikipedia.org)
- Local factors, including transcription factors such as SRY-box 9 protein (SOX9), Runt-related transcription factor 2 (RUNX2), and bone morphogenetic proteins (BMPs), along with signaling pathways such as the Wnt pathway, play critical roles in chondrocyte proliferation and differentiation. (pfmjournal.org)
- Hypertrophic chondrocytes, a progeny of terminally differentiated chondrocytes from the proliferative zone, cease further proliferation, undergo hypertrophy in columns parallel to the axis of longitudinal elongation, and initiate the production of factors that trigger mineralization and vessel invasion. (pfmjournal.org)
- Growth hormone (GH), sex hormone, thyroid hormone, as well as glucocorticoids affect chondrocyte proliferation and hypertrophy. (pfmjournal.org)
Hypertrophy1
- While hypertrophy of chondrocytes is a physiological process implicated in the longitudinal growth of long bones, hypertrophy-like alterations in chondrocytes play a major role in OA. (biomedcentral.com)
Proliferative3
- Autologous chondrocytes implantation and osteochondral autograft transplantation are limited by scarce cartilage production, low proliferative capacity of chondrocytes, chondrocyte de-differentiation and complications due to donor site morbidity 5 . (nature.com)
- The growth plate can be stratified into three distinct zones based on the size, morphology, orientation, proliferative potential, and function of chondrocytes: resting, proliferative, and hypertrophic zone. (pfmjournal.org)
- Explant studies from the in vitro fixation model confirmed the long-term viability and proliferative capacity of these chondrocytes. (biomedcentral.com)
Differentiate1
- The expression of Sox9 induces the expression of BMP, which causes chondrocytes to proliferate and differentiate. (wikipedia.org)
Osteoarthritic1
- In this study we compared the quantitative changes in proteins synthesized in osteoarthritic compared to normal chondrocytes. (biomedcentral.com)
Hypertrophic1
- In an experimental mouse OA model using a microsurgical technique, MMP-13 levels correlate with the presence of pathological chondrocytes that undergo hypertrophic differentiation in the early stage of OA development [ 11 ] and its over-expression can induce the onset of OA through excessive ECM degradation [ 12 ]. (biomedcentral.com)
Proteins3
- We identified several pathways and proteins to be associated with OA chondrocytes. (biomedcentral.com)
- Transfection of a miR-181b inhibitor could increase the expression levels of the ferroptosis-related proteins solute carrier family 7 members 11 (SLC7A11) and glutathione peroxidase 4 (GPX4), thereby inhibiting the occurrence of ferroptosis in chondrocytes. (biomedcentral.com)
- MATN1 and MATN3 are expressed specifically in cartilage and are among the most up-regulated ECM proteins during chondrogenesis. (ayurvedandindia.com)
Cartilage matrix3
- We show that LNA043 promotes chondrogenesis and cartilage matrix synthesis in vitro and regenerates hyaline articular cartilage in preclinical OA and cartilage injury models in vivo. (nih.gov)
- It was subdivided into type IA, which has apparently normal cartilage matrix but inclusions in chondrocytes, and type IB, which has an abnormal cartilage matrix. (medscape.com)
- Achondrogenesis type IB has a cartilage matrix that shows coarsened collagen fibers that are particularly dense around the chondrocytes, forming collagen rings. (medscape.com)
Bone4
- The additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation is thought to enhance cartilage repair as it may provide both an additional cell population capable of chondrogenesis and a source of growth factors stimulating cartilage repair. (hindawi.com)
- Moreover, the BMA clot provides a three-dimensional environment, possibly further supporting chondrogenesis and protecting the subchondral bone from structural alterations. (hindawi.com)
- This review provides an overview of the structural aspects of the growth plate, factors influencing chondrocyte function, and their impact on longitudinal bone growth. (pfmjournal.org)
- Vacuolated chondrocytes, intrachondrocytic inclusion bodies (periodic acid-Schiff stain [PAS] positive, diastase resistant), extraskeletal cartilage involvement, enlarged lacunas, and woven bone are all present. (medscape.com)
Metabolism4
- Chondrocytes, form cartilage in synovial joints and growth plates, respond to compressive force by activating their metabolism and progressing differentiation, while tensile stimulation inhibits their differentiation. (elsevierpure.com)
- Mechanical stimulation is translated into intracellular signaling, which regulates the differentiation state and metabolism of chondrocytes. (elsevierpure.com)
- Growth, metabolism, and differentiation of chondrocytes, which are the key cellular players in this process, are regulated by systemic hormones, local factors, and cellular signaling pathways. (pfmjournal.org)
- Chondrocyte growth, metabolism, and differentiation are complex processes. (pfmjournal.org)
Chondrogenic2
- Our data demonstrated that LMHF mechanical vibration promotes BMSCs chondrogenic differentiation and implies β-catenin signal acts as an essential mediator in the mechano-biochemical transduction and subsequent transcriptional regulation in the process of chondrogenesis. (qigonginstitute.org)
- articular pdf change( airborne chondrocytes mimic available for chondrogenic Operation in their tissue to covalently sue in exercise testing understanding, fruit, and cartilage without injured vast materials. (a-e-markt.de)
Phenotype1
- Induction of chondrogenesis and maintenance of the chondrocyte phenotype are critical events for autologous periosteal transplantation, which is a viable approach for cartilage repair. (firebaseapp.com)
Inflammation1
- This pilot study aimed to investigate the effect of characterized MSC-EVs on chondrogenesis in a 3D chondrocyte inflammation model with the pro-inflammatory cytokine TNFα. (bvsalud.org)
Oxidative stress1
- Importantly, GPX4 downregulation could increase the sensitivity of chondrocytes to oxidative stress and improve OA-like changes in chondrocytes [ 10 ]. (biomedcentral.com)
Pathways involved1
- In this review, we summarized key regulatory factors and signaling pathways involved in chondrocyte functions, to understand how they affect chondrocyte growth and development. (pfmjournal.org)
Bovine1
- Methods: Bovine articular chondrocytes were expanded and transferred into pellet culture at passage 3. (bvsalud.org)
Collagen2
- A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. (lookformedical.com)
- No collagen rings are present around the chondrocytes. (medscape.com)
Differentiation potential1
- Histologic and immunohistochemical evaluations indicated a higher differentiation potential of chondrocytes treated with 84-EVi. (bvsalud.org)
Articular cartilage1
- Chondrocytes were isolated from articular cartilage of ten patients with primary OA undergoing knee replacement surgery and six normal donors undergoing fracture repair surgery without history of joint disease and no OA clinical manifestations. (biomedcentral.com)
Alterations1
- In this review, we highlight the main MMP-13-related changes in OA chondrocytes, including alterations in the activity and expression level of MMP-13 by upstream regulatory factors, DNA methylation, various non-coding RNAs (ncRNAs), and autophagy. (biomedcentral.com)
Vivo2
- However, more direct evidence using an animal model is necessary to demonstrate the in vivo role of ESRRa in chondrocyte development. (nature.com)
- Cartilage is an avascular tissue, and chondrocytes in vivo experience a severely hypoxic environment. (ox.ac.uk)
Regulate1
- Research is still ongoing and novel transcription factors, such as ATOH8 and EBF1, are added to the list of genes that regulate chondrogenesis. (wikipedia.org)
Chondral1
- ACI is a two-step surgical procedure which necessitates chondral harvesting from a non-weight-bearing area of the knee and external chondrocytes expansion in a dedicated laboratory [ 9 , 10 ]. (springer.com)
Mineralization1
- Although the mechanisms of these effects are not known, the results suggest that local lead-related effects on growth-plate chondrogenesis and metaphyseal mineralization may be involved. (duke.edu)
Inclusions1
- This distinguishes type IB from type IA, in which the matrix is close to normal and inclusions can be seen in chondrocytes, and from achondrogenesis type II, in which cationic dyes give a normal staining pattern. (medscape.com)
Aberrant1
- This study aimed to investigate the correlation between the aberrant upregulation of miR-181b and ferroptosis in chondrocytes during the progression of OA. (biomedcentral.com)
Viability1
- Transfection of SLC7A11 siRNA effectively abrogated the increase in chondrocyte viability induced by the miR-181 inhibitor and increased ferroptosis. (biomedcentral.com)
Vessel1
- Kritikpunkte ein vessel chondrocyte theory Seite Dies zu verbessern. (a-e-markt.de)
Cultures2
- Primary cell cultures obtained from gonarthrosis patients were divided into four groups, two of which were designated as control cultures of chondrocyte and osteocyte, and the other two groups were exposed to BAs administered via the culture medium. (biomedcentral.com)
- Skin and cartilage biopsies for fibroblast and chondrocyte cultures allow study of sulfate incorporation. (medscape.com)
Cell1
- used in B-cell-inhibiting treatments on human primary chondrocytes and osteocytes in vitro ). (biomedcentral.com)