• MSCs undergoing chondrogenesis are preferentially responsive to an electromagnetic efficacy window defined by field amplitude, duration and frequency of exposure. (nature.com)
  • MSCs can be differentiated along different cell lineages of mesodermal origin including osteoblasts, chondrocytes, skeletal myocytes or visceral stromal cells 9 . (nature.com)
  • This correlated with enhanced chondrogenesis and a reduction in the expression of markers associated with chondrocyte hypertrophy, as well as increased SMAD 2/3 nuclear localization in the encapsulated MSCs. (amrita.edu)
  • Mesenchymal stem cells (MSCs) are well known to have the capability to form bone and cartilage, and chondrogenesis derived from MSCs is reported to be affected by mechanical stimuli. (qigonginstitute.org)
  • Mobilized pluripotent MSCs from the subchondral bone migrate into the defect filled with the clot, differentiate into chondrocytes and osteoblasts, and form a repair tissue over time. (hindawi.com)
  • MSCs have multilineage differentiation potential, allowing them to differentiate, for example, into chondrocytes and osteoblasts, the key cells from the two tissues that constitute the osteochondral unit. (hindawi.com)
  • In the orthopaedic field, additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation has been recently studied, since the bone marrow itself is both a source of MSCs, providing a cell population capable of chondrogenesis and of various growth factors stimulating cartilage repair [ 7 - 10 ]. (hindawi.com)
  • Moreover, the bone marrow clot provides a three-dimensional (3D) environment which supports the chondrogenesis of MSCs. (hindawi.com)
  • In contrast to the cost- and labor-intensive cultivation and propagation of cells such as MSCs or articular chondrocytes, the clinical use of "minimally processed" autologous BMA that can be prepared in the operation room as a single-step procedure appears straightforward. (hindawi.com)
  • Introduction: Mesenchymal stem cells (MSCs) can be isolated from adult bone marrow (BM), expanded, and differentiated into several cell types, including chondrocytes. (elsevierpure.com)
  • The aim of our study was to define the biological role of IGF-I on proliferation, chondrogenic condensation, apoptosis, and differentiation of MSCs into chondrocytes, alone or in combination with TGF-β and in the presence or absence of TGF-β signaling. (elsevierpure.com)
  • Our data, by showing the role of IGF-I and TGF-β1 in the critical steps of MSC chondrogenesis, provide critical information to optimize the therapeutic use of MSCs in cartilage disorders. (elsevierpure.com)
  • Description: Matrilins (MATNs) are a family of non-collagenous extra-cellular matrix (ECM) proteins consisting of four known members that have been proposed to play key roles in modulating cellular phenotypes during chondrogenesis of mesenchymal stem cells (MSCs). (ayurvedandindia.com)
  • Bone morphogenetic proteins are growth factors released during embryonic development to induce condensation and determination of cells, during chondrogenesis. (wikipedia.org)
  • In the present study, the potential of using exosomes isolated from adipose‑derived stem cells (ADSCs) as a therapeutic tool for reducing chronic inflammation and promoting chondrogenesis was investigated using patient‑derived primary cells. (spandidos-publications.com)
  • In vitro expansion of adipose-derived adult stromal cells in hypoxia enhances early chondrogenesis. (ox.ac.uk)
  • Using a defined in vitro model of early chondrogenesis, we attempted to enrich for cells with an enhanced ability for chondrogenic differentiation by pre-exposure of mouse adipose-derived adult stromal cells (ADASs) to a hypoxic (2% oxygen) environment. (ox.ac.uk)
  • The resting zone, a source of stemlike progenitor cells that restores the reservoir of proliferative chondrocytes, is located farthest from the primary ossification center and assumes responsibility for preserving the architectural integrity of the growth plate [ 4 ]. (pfmjournal.org)
  • Human hyaline cartilage is comprised of a unique profile of chondrocytes (cells), matrix proteins (signals), and tissue architecture (scaffold), which together this advanced structure-function relationship imparts critical properties to the joint spaces such as cushioning and lubrication [ 5 ]. (biomedcentral.com)
  • We performed a quantitative proteomic analysis in osteoarthritic and normal chondrocytes followed by functional analyses to investigate proteome changes and molecular pathways involved in OA pathogenesis. (biomedcentral.com)
  • However, this was accompanied by increased markers of chondrogenesis. (qigonginstitute.org)
  • The protein expression of chondrocyte-specific markers of Aggrecan, Sox9, and BMP7 were upregulated and Collagen X was decreased by LMHF vibration introduced at the chondrogenic differentiation in vitro. (qigonginstitute.org)
  • This study provides evidence for further testing on the molecular mechanism of the disease and also propose proteins as candidate markers of OA chondrocyte phenotype. (biomedcentral.com)
  • The protein expression of the human chondrocyte markers Aggrecan and collagen II was identified by immunofluorescence, according to Li et al. (biomedcentral.com)
  • This study highlights the intricacies of calcium homeostasis during early chondrogenesis and the constraints that are placed on PEMF-based therapeutic strategies aimed at promoting MSC chondrogenesis. (nature.com)
  • Furthermore, re-oxygenation of the 2% oxygen-expanded ADASs before differentiation did not significantly affect early chondrogenesis. (ox.ac.uk)
  • Ihh stimulates chondrocyte proliferation and regulates chondrocyte maturation by maintaining the expression of PTHrP. (wikipedia.org)
  • Local factors, including transcription factors such as SRY-box 9 protein (SOX9), Runt-related transcription factor 2 (RUNX2), and bone morphogenetic proteins (BMPs), along with signaling pathways such as the Wnt pathway, play critical roles in chondrocyte proliferation and differentiation. (pfmjournal.org)
  • Hypertrophic chondrocytes, a progeny of terminally differentiated chondrocytes from the proliferative zone, cease further proliferation, undergo hypertrophy in columns parallel to the axis of longitudinal elongation, and initiate the production of factors that trigger mineralization and vessel invasion. (pfmjournal.org)
  • Growth hormone (GH), sex hormone, thyroid hormone, as well as glucocorticoids affect chondrocyte proliferation and hypertrophy. (pfmjournal.org)
  • Autologous chondrocytes implantation and osteochondral autograft transplantation are limited by scarce cartilage production, low proliferative capacity of chondrocytes, chondrocyte de-differentiation and complications due to donor site morbidity 5 . (nature.com)
  • The growth plate can be stratified into three distinct zones based on the size, morphology, orientation, proliferative potential, and function of chondrocytes: resting, proliferative, and hypertrophic zone. (pfmjournal.org)
  • Explant studies from the in vitro fixation model confirmed the long-term viability and proliferative capacity of these chondrocytes. (biomedcentral.com)
  • The expression of Sox9 induces the expression of BMP, which causes chondrocytes to proliferate and differentiate. (wikipedia.org)
  • In an experimental mouse OA model using a microsurgical technique, MMP-13 levels correlate with the presence of pathological chondrocytes that undergo hypertrophic differentiation in the early stage of OA development [ 11 ] and its over-expression can induce the onset of OA through excessive ECM degradation [ 12 ]. (biomedcentral.com)
  • The additional application of a bone marrow aspirate (BMA) to the procedure of marrow stimulation is thought to enhance cartilage repair as it may provide both an additional cell population capable of chondrogenesis and a source of growth factors stimulating cartilage repair. (hindawi.com)
  • Moreover, the BMA clot provides a three-dimensional environment, possibly further supporting chondrogenesis and protecting the subchondral bone from structural alterations. (hindawi.com)
  • This review provides an overview of the structural aspects of the growth plate, factors influencing chondrocyte function, and their impact on longitudinal bone growth. (pfmjournal.org)
  • Vacuolated chondrocytes, intrachondrocytic inclusion bodies (periodic acid-Schiff stain [PAS] positive, diastase resistant), extraskeletal cartilage involvement, enlarged lacunas, and woven bone are all present. (medscape.com)
  • Chondrocytes, form cartilage in synovial joints and growth plates, respond to compressive force by activating their metabolism and progressing differentiation, while tensile stimulation inhibits their differentiation. (elsevierpure.com)
  • Mechanical stimulation is translated into intracellular signaling, which regulates the differentiation state and metabolism of chondrocytes. (elsevierpure.com)
  • Growth, metabolism, and differentiation of chondrocytes, which are the key cellular players in this process, are regulated by systemic hormones, local factors, and cellular signaling pathways. (pfmjournal.org)
  • Chondrocyte growth, metabolism, and differentiation are complex processes. (pfmjournal.org)
  • Our data demonstrated that LMHF mechanical vibration promotes BMSCs chondrogenic differentiation and implies β-catenin signal acts as an essential mediator in the mechano-biochemical transduction and subsequent transcriptional regulation in the process of chondrogenesis. (qigonginstitute.org)
  • articular pdf change( airborne chondrocytes mimic available for chondrogenic Operation in their tissue to covalently sue in exercise testing understanding, fruit, and cartilage without injured vast materials. (a-e-markt.de)
  • This pilot study aimed to investigate the effect of characterized MSC-EVs on chondrogenesis in a 3D chondrocyte inflammation model with the pro-inflammatory cytokine TNFα. (bvsalud.org)
  • In this review, we summarized key regulatory factors and signaling pathways involved in chondrocyte functions, to understand how they affect chondrocyte growth and development. (pfmjournal.org)
  • Methods: Bovine articular chondrocytes were expanded and transferred into pellet culture at passage 3. (bvsalud.org)
  • Histologic and immunohistochemical evaluations indicated a higher differentiation potential of chondrocytes treated with 84-EVi. (bvsalud.org)
  • Chondrocytes were isolated from articular cartilage of ten patients with primary OA undergoing knee replacement surgery and six normal donors undergoing fracture repair surgery without history of joint disease and no OA clinical manifestations. (biomedcentral.com)
  • In this review, we highlight the main MMP-13-related changes in OA chondrocytes, including alterations in the activity and expression level of MMP-13 by upstream regulatory factors, DNA methylation, various non-coding RNAs (ncRNAs), and autophagy. (biomedcentral.com)
  • However, more direct evidence using an animal model is necessary to demonstrate the in vivo role of ESRRa in chondrocyte development. (nature.com)
  • Cartilage is an avascular tissue, and chondrocytes in vivo experience a severely hypoxic environment. (ox.ac.uk)
  • ACI is a two-step surgical procedure which necessitates chondral harvesting from a non-weight-bearing area of the knee and external chondrocytes expansion in a dedicated laboratory [ 9 , 10 ]. (springer.com)
  • Although the mechanisms of these effects are not known, the results suggest that local lead-related effects on growth-plate chondrogenesis and metaphyseal mineralization may be involved. (duke.edu)
  • This distinguishes type IB from type IA, in which the matrix is close to normal and inclusions can be seen in chondrocytes, and from achondrogenesis type II, in which cationic dyes give a normal staining pattern. (medscape.com)
  • This study aimed to investigate the correlation between the aberrant upregulation of miR-181b and ferroptosis in chondrocytes during the progression of OA. (biomedcentral.com)
  • Transfection of SLC7A11 siRNA effectively abrogated the increase in chondrocyte viability induced by the miR-181 inhibitor and increased ferroptosis. (biomedcentral.com)
  • Kritikpunkte ein vessel chondrocyte theory Seite Dies zu verbessern. (a-e-markt.de)
  • Primary cell cultures obtained from gonarthrosis patients were divided into four groups, two of which were designated as control cultures of chondrocyte and osteocyte, and the other two groups were exposed to BAs administered via the culture medium. (biomedcentral.com)
  • Skin and cartilage biopsies for fibroblast and chondrocyte cultures allow study of sulfate incorporation. (medscape.com)