Cholesterol cho cells. Medical search. Frequent questions

Cholesterol is a lipid that gets bad press because of its association with cardiovascular disease," says Wonhwa Cho, professor of chemistry at UIC, who led the research. (analytica-world.com)
Lecture] Distinguished Lectureship Series Seminar by Prof. Wonhwa Cho (Univ. (yonsei.ac.kr)
Prof. Wonhwa Cho (Univ. (yonsei.ac.kr)

Cho S, Lee H, Seo J (2021) Impact of genetic risk factors for Alzheimer's disease on brain glucose metabolism. (asiaresearchnews.com)

He was awarded the Nobel Prize in Physiology or Medicine with Joseph L. Goldstein in 1985 for describing the regulation of cholesterol metabolism. (wikipedia.org)
citation needed] Moving to the University of Texas Michael liked vann Warner Health Science Center in Dallas, now the UT Southwestern Medical Center, Brown and colleague Joseph L. Goldstein researched cholesterol metabolism and discovered that human cells have low-density lipoprotein (LDL) receptors that extract cholesterol from the bloodstream. (wikipedia.org)
Brain cholesterol metabolism is segregated from the peripheral circulation by the blood-brain barrier (BBB) and cholesterol transport in the central nervous system (CNS) is operated by lipoproteins very similar to the circulating high-density lipoproteins (HDLs) and called "HDL-like particles" [ 1 ]. (biomedcentral.com)
These results suggest that Mal-ßCD helps to maintain normal lipid metabolism by restoring balance among UEC, ECs, and LCFAs through acting on behalf of NPC1 in Npc1-KO cells and may therefore be useful in designing effective therapies for NPC. (bvsalud.org)
Troubleshooting tips were on protein glycosylation, fatty acid and cholesterol supplementation, using metabolism characteristics to improve production, reducing apoptosis , increasing cell viability, and handling waste issues. (cellculturedish.com)
Can you explain how cell metabolism characteristics can be used to optimize media in CHO cells? (cellculturedish.com)
There are several ways of utilizing cell metabolism characteristics for medium optimization. (cellculturedish.com)
Since metabolic characteristics are dynamic, utilization of metabolism rates measured from both the cell growth phase and stationary production phase are best for stoichiometric balancing of basal and feed media. (cellculturedish.com)
Finally, cell metabolism characteristics can also impact the balance between biomass formation and protein production. (cellculturedish.com)
For a cell line with high growth rate but low productivity, limiting the metabolism rates through medium optimization can force the cells work on protein production thereby improving product yield. (cellculturedish.com)
Several unexpected findings in genetically altered mice have prompted research into the direction of intermediate metabolism of skeletal cells. (mdpi.com)
Protein kinases are involved in signal transduction pathways linking growth factors, hormones and other cell regulation molecules to cell growth, survival and metabolism under both normal and pathological conditions. (justia.com)

Cholesterol transport proteins (blue) can alter its distribution between the inner and outer layers. (analytica-world.com)
Cho's earlier studies showed cholesterol interacts with many regulatory molecules - mostly cellular proteins - but it was never thought to be one. (analytica-world.com)
Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of therapeutic proteins. (labroots.com)
To identify cholesterol-respon-sive proteins likely responsible for the larval arrest upon CS, a comparative proteomic analysis was performed between C. elegans grown in normal medium supplemented with cholesterol (CN) and those grown in medium not supplemented with cholesterol (cholesterol starvation, CS). (molcells.org)

The results were recapitulated in a study of the human LDLR promoter coupled to the luciferase gene in CHO cells under the same cell culture conditions. (abt737.com)

To determine TRPA1 localization in cellular membranes we stained HEK293T cells transfected with the mTRPA1-mCherry construct with the Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit. (elifesciences.org)
It is important to consider that both MCD and MCD/Cholesterol complexes likely modify the cholesterol content in internal membranes. (healthycholesterolclub.com)
It is considered somewhat above plantlife because it does not have the hard cellulose membranes that are characteristic of plant cells- nor does it have a well defined nucleus. (metacenterchicago.com)
Furthermore, permeability was found to be highly temperature-dependent in Chinese hamster ovary (CHO) control cells and artificial membranes (parallel artificial membrane permeability assay). (aspetjournals.org)

China (12KJB320009), and the Research Project of the Technology and Technology Bureau of Suzhou City of P.R. examine the part of Ambra1 in apoptosis, Ambra1 knockdown cells were treated with staurosporine and etoposide. (rawveronica.com)
Cell apoptosis and viability were measured by annexin-V and PI staining and MTT assays. (rawveronica.com)
CTSB also plays significant roles in immune responses including both T and B cell apoptosis and Th1/Th2 polarization. (biolegend.com)
Natural product anticancer agents enhance intracellular levels of ceramide, a sphingolipid that promotes cell apoptosis. (sphingolipidclub.com)
What media components can reduce apoptosis and increase cell viability beyond critical density? (cellculturedish.com)
Also, PKB is overexpressed in 15% of ovarian cancers, 12% of pancreatic cancers and 3% of breast cancers, and was shown to produce a survival signal that protects cells from apoptosis thus contributing to resistance to chemotherapy. (justia.com)
Ideally, a drug that inhibits PKB should cause both cell cycle arrest and promote apoptosis. (justia.com)

The membrane contains up to 90 percent of a cell's total cholesterol, and cholesterol makes up about 40 percent of the membrane lipids. (analytica-world.com)
AD patients have normal plasma lipids but significantly reduced unesterified cholesterol and unesterified/total cholesterol ratio. (biomedcentral.com)
The CSF unesterified to total cholesterol ratio was increased in AD patients, and CSF CER and CEC from astrocytes were significantly reduced in AD patients. (biomedcentral.com)
In the AD group, a significant positive correlation was observed between plasma unesterified cholesterol and unesterified/total cholesterol ratio with Aβ 1-42 CSF content. (biomedcentral.com)
Taken together our data indicate that cholesterol esterification is hampered in plasma and CSF of AD patients and that plasma cholesterol esterification biomarkers (unesterified cholesterol and unesterified/total cholesterol ratio) are significantly associated to disease biomarkers (i.e. (biomedcentral.com)
While not all research agrees, the most reliable evidence shows that taking garlic may reduce total cholesterol and low-density lipoprotein (LDL, "bad" cholesterol) in people with high cholesterol levels. (supplemented.net)
The controlling nutritional status (CONUT) score, which is calculated based on patients' serum albumin, total lymphocytes and total cholesterol, is associated with Full Nutritional Assessment (FNA) and was recently introduced as a nutritional screening tool 12 . (nature.com)
There is interest in using amaranth for high cholesterol because some research in animals suggests that it might be able to lower total cholesterol and "bad" LDL cholesterol, while raising "good" HDL cholesterol . (rxlist.com)

Blood cell DNA methylation biomarkers in preclinical malignant pleural mesothelioma: The EPIC prospective cohort. (who.int)

They made the surprising finding that, in addition to its many other biological roles, cholesterol is a signaling molecule that transmits messages across the cell membrane. (analytica-world.com)
But in this paper, we showed that a single cholesterol molecule can itself be the signal trigger," Cho said. (analytica-world.com)
The present invention provides inhibitors of protein kinases comprising a molecule having at least a first moiety competent for penetration of the molecule into cells, and a second moiety for having a protein kinase inhibiting effect within the cells. (justia.com)
Although my area of expertise is brain function, I didn't know anything about choles-terol, aside from what we all learned in biology, which is that cholesterol, a steroid or lipid, is an essential molecule of life. (westonaprice.org)
HG: If cholesterol is an essential molecule for life, why has it been maligned as the root cause of heart disease? (westonaprice.org)
The GH molecule binds to its specific cell surface receptor (GHR), which dimerizes with another GHR molecule so that the single GH molecule is enveloped by 2 GHR molecules. (medscape.com)

Their findings led to the development of statin drugs, the cholesterol-lowering compounds that today are used by 16 million Americans and are the most widely prescribed medications in the United States. (wikipedia.org)
citation needed] New federal cholesterol guidelines will triple the number of Americans taking statin drugs to lower their cholesterol, reducing the risk of heart disease and stroke for countless people. (wikipedia.org)

Recent innovations in single-cell Assay for Transposase Accessible Chromatin using sequencing (scATAC-seq) enable profiling of the epigenetic landscape of thousands of individual cells. (biotech2012.org)
In view of these considerations, this study was designed to evaluate the cytotoxic and genotoxic potential of geraniol, in an in vitro protocol, using two types of human cells: one without the ability to metabolize (peripheral blood mononuclear cells - PBMC), and the other with this capability (human hepatoma cell line - HepG2) through the comet assay and the micronucleus test. (geneticsmr.org)
Four concentrations (10, 25, 50, and 100 μg/mL) were selected for the genotoxic assessment for PBMC and three (1.25, 2.5, and 5 μg/mL) for HepG2 cells based on cytotoxicity tests (MTT assay). (geneticsmr.org)

Dyslipidemia Dyslipidemia is elevation of plasma cholesterol, triglycerides (TGs), or both, or a low high-density lipoprotein cholesterol (HDL-C) level that contributes to the development of atherosclerosis. (msdmanuals.com)

BACKGROUND: Niemann-Pick disease type C (NPC) is a fatal neurodegenerative disorder caused by abnormal intracellular cholesterol trafficking. (bvsalud.org)
The ability of CD derivatives to form 1:1 and 2:1 complexes with UC were correlated with their ability to normalize intracellular cholesterol trafficking serving as shuttle and with their cytotoxicity associated with cellular UC efflux acting as sink, respectively, in NPC model cells. (bvsalud.org)
Niemann-Pick disease type C (NPC) is a fatal disorder with abnormal intracellular cholesterol trafficking resulting in neurodegeneration and hepatosplenomegaly. (bvsalud.org)

Niemann-Pick disease Type C (NPC) is a lysosomal storage disorder caused by mutation of the NPC1/NPC2 genes, which ultimately results in the accumulation of unesterified cholesterol (UEC) in lysosomes, thereby inducing symptoms such as progressive neurodegeneration and hepatosplenomegaly. (bvsalud.org)
To investigate whether ABCA2 was coordinately regulated with other sterol-responsive genes, we measured the mRNA levels of several genes in HepG2 liver hepatocarcinoma cells cultured in lipoprotein-deficient medium (LPDS) and in medium supplemented with exogenous sterols. (abt737.com)
The mRNA levels of both the Low-density lipoprotein receptor (LDLR) and Hydroxymethylglutaryl coenzyme A synthase (HMGCS) genes are sensitive to changes in the level of cellular cholesterol. (abt737.com)
Transcription of these genes is increased when cells are cultured in lipoprotein-deficient medium and repressed when exogenous sterols are added to the culture medium. (abt737.com)
To determine whether the constitutive overexpression of ABCA2 in cells alters the expression of other sterol-responsive genes, we generated a CHO cell line that constitutively overexpressed human ABCA2 under control of the cytomegalovirus (CMV) promoter control element. (abt737.com)
These findings indicated that constitutive overexpression of ABCA2 in cells was sufficient to increase the expression of genes involved in cholesterol homeostasis. (abt737.com)

inhibition of cholesterol trafficking from the LE/LY to cholesterol sensors in the endoplasmic reticulum would activate the cholesterol synthetic pathway and sterol-responsive gene expression would be up regulated. (abt737.com)

ATI-5261 treatment also increased serum ABCA1 concentrations and cholesterol efflux capacity, as well as ABCA1 expressions in the placenta. (biomedcentral.com)
We hypothesized that constitutive overexpression of ABCA2 in CHO cells in culture medium containing serum (a source of LDL-derived free cholesterol) would mimic sterol-deprivation. (abt737.com)
CHO, MDCK and BHK cell lines were discussed and overall topics included media formulation matters like powder vs. liquid, stability, solubility, serum-free, chemically defined and non-defined components, and component sources. (cellculturedish.com)
The complex molecules are preferably peptide conjugates having improved cell-permeability, serum stability and kinase selectivity compared to known protein kinase inhibitors. (justia.com)

Lipoprotein profile, cholesterol esterification, and cholesterol efflux capacity (CEC) were evaluated in plasma and CSF. (biomedcentral.com)
In agreement with the reduced preβ-HDL particles, cholesterol efflux capacity mediated by the transporters ABCA1 and ABCG1 was reduced in AD patients' plasma. (biomedcentral.com)
Cyclodextrins (CDs), the most promising therapeutic candidates for NPC, but with concerns about ototoxicity, are cyclic oligosaccharides with dual functions of unesterified cholesterol (UC) shuttle and sink that catalytically enhance the bidirectional flux and net efflux of UC, respectively, between the cell membrane and the extracellular acceptors. (bvsalud.org)
Cholesterol efflux capacity, especially ATP binding cassette transporter A1 (ABCA1) dependent capacity, has been proposed to be a likely mediator of arterial stiffness. (biomedcentral.com)
Therefore, cholesterol efflux has been proposed to be a likely mediator of arterial stiffness supported by increasing evidence [ 11 ]. (biomedcentral.com)
The initial and rate-limiting step of cholesterol efflux is the transport of cellular free cholesterol to high-density lipoprotein (HDL) by cholesterol transporters, of which the best characterized are the ATP binding cassette transporter (ABC) A1, ABCG1 and scavenger receptor BI (SR-BI) [ 12 ]. (biomedcentral.com)
Apolipoprotein (Apo) A1 is the major component of HDL protein that facilitates cholesterol efflux from peripheral cells through ABCA1. (biomedcentral.com)
These cells not only demonstrated a 3-foldincrease in resistance to doxorubicin and a 9-fold increase in resistance to paclitaxel, but also showed increased efflux of rhodamine. (sphingolipidclub.com)

The detection of harmful stimuli such as noxious chemicals and changes in temperature and pressure are fundamental biological processes carried out by numerous cell types through diverse specialized receptors. (elifesciences.org)
The lack of sufficient LDL receptors is implicated in familial hypercholesterolemia, which predisposes heavily for cholesterol-related diseases. (wikipedia.org)
APOE binds to cell-surface receptors to deliver lipids and to the hydrophobic amyloid-β peptide, regulating amyloid-β aggregations and clearances in the brain. (dovepress.com)

The mRNA of the transcription factor, Sterol response element binding protein-2 (SREBP2), is elevated in cells under sterol-depleted conditions and repressed upon sterol loading [47]. (abt737.com)
When an U18666a-derivative, synthesized to contain an ultraviolet light-sensitive benzophenone moiety was added to CHO cells, exposure to UV light induced the crosslinking of the derivative to the sterol-sensing domain (SSD) of the Niemann Pick Type C1 protein (NPC1), abrogating its activity [51]. (abt737.com)
CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. (labroots.com)
Chemerin is an adipocytokine involved in the recruitment of immune cells [ 11 ] and serves as a bridge between various immune cells from the initial stage of inflammatory diseases through the development process [ 12 ], and its receptor CMKLR1 (also named ChemR23 in humans) is a specific functional member of the G protein receptor family. (biomedcentral.com)
The present invention relates to cell permeable, stable conjugates comprising a cell-permeability enhancement moiety and a peptide or peptidomimetic, as selective inhibitors of protein kinases, to pharmaceutical compositions containing them, as well as to processes for the preparation and use of such complex molecules. (justia.com)

It's also used for high levels of cholesterol or other fats (lipids) in the blood, diabetes, obesity, and many other conditions. (medlineplus.gov)
These conditions include high blood pressure, high levels of cholesterol or other fats (lipids) in the blood (hyperlipidemia), and hardening of the arteries (atherosclerosis). (supplemented.net)
High levels of cholesterol or other fats (lipids) in the blood (hyperlipidemia). (supplemented.net)

And taking garlic doesn't help increase high-density lipoprotein (HDL, "good" cholesterol) or lower levels of other blood fats called triglycerides. (supplemented.net)
I knew all that, but I was also concerned about my HDL cholesterol being low and my triglycerides being high. (westonaprice.org)

Cell Rep https://doi:10.1016/j.celrep.2020.108224 . (asiaresearchnews.com)
Cancer Lett, 300:215-224.doi:10.1016/j. squamous cell carcinoma in north-eastern Iran. (who.int)

starting place by taking the common of concentrations found in a prior CHO cell lifestyle research [9], as no guide concentration is designed for vacuum infiltration of kifunensine. (rawveronica.com)

Clear cell renal cell carcinoma (CCRCC) is a renal cortical tumor typically characterized by malignant epithelial cells with clear cytoplasm and a compact-alveolar (nested) or acinar growth pattern interspersed with intricate, arborizing vasculature. (medscape.com)
Typical gross presentation of clear cell renal cell carcinoma (lower left) with golden color due to intracellular lipid accumulation. (medscape.com)
See Renal Cell Carcinoma: Recognition and Follow-up , a Critical Images slideshow, to help evaluate renal masses and determine when and what type of follow-up is necessary. (medscape.com)
Go to Renal Cell Carcinoma and Sarcomatoid and Rhabdoid Renal Cell Carcinoma for more complete information on these topics. (medscape.com)
The incidence of renal cell carcinoma (RCC) has been rising steadily in Europe and the United States for the past 3 decades, with a particular rise in the proportion of small, asymptomatic tumors detected incidentally via abdominal imaging. (medscape.com)
Smoking, obesity, and hypertension are the 3 most well-established risk factors associated with development of sporadic renal cell carcinoma (RCC). (medscape.com)
The renal tumors are frequently multifocal and/or bilateral and are always of the clear cell renal cell carcinoma (CCRCC) histologic type. (medscape.com)
E6 and Metabolic syndrome and risks of colon and rectal renal cell carcinoma. (who.int)

We found that the initial response of endothelial cells to changes in substrate elasticity was determined by membrane cholesterol levels, and that the extent of endothelial cell spreading increases with membrane cholesterol content. (missouri.edu)
Cytolethal distending toxin family members are differentially affected by alterations in host glycans and membrane cholesterol. (riken.jp)

Progesterone and U18666a increase expression of ABCA2 mRNA To gain insight into a functional role for ABCA2, we treated cells with agents that are known to modulate cholesterol homeostasis in the LE/LY. (abt737.com)
More recent work has elucidated the mechanism of U18666a function in inhibiting cholesterol exit from the LE/LY. (abt737.com)
Treatment of CHO cells with either progesterone or U18666a resulted in an elevation of ABCA2 mRNA expression over 2-fold and 3.5-fold, respectively [46]. (abt737.com)

In this study, the biomechanical response of bovine aortic endothelial cells (BAECs) with different membrane cho- lesterol levels to polyacrylamide (PA) gels was investigated by measuring cell adhesion and spreading behaviors at varying PA elasticity. (missouri.edu)

The purpose of this study was to evaluate cholesterol esterification and HDL subclasses in plasma and cerebrospinal fluid (CSF) of Alzheimer's disease (AD) patients. (biomedcentral.com)

In this study, we examined the ability of vasoactive agonists to induce dynamic changes in vascular smooth muscle cell (VSMC) elasticity and adhesion, and tested the hypothesis that these events are coordinated with rapid remodelling of the cortical cytoskeleton. (missouri.edu)

Several anti-diabetic strategies including adiponectin, ezetimibe, liraglutide, taurine, adipose tissue-derived stem cells (ADSCs) and even exercise as well as natural products such GABA tea, geniposide, guava extract, vitamin D have been shown to target autophagy. (frontiersin.org)
Such activity would result in increased cell death of tumor tissue where PKB is elevated, and in decreased resistance to chemotherapy agents. (justia.com)

In this study, we show that mouse TRPA1 is located preferably in cholesterol-rich domains and identify cholesterol recognition amino acid consensus (CRAC) motifs in the TM2 and TM4 segments that are implicated in the attenuation of chemical activation of mTRPA1 by cholesterol-depleting agents. (elifesciences.org)

This study determines the effects of 6-O-α-maltosyl-ß cyclodextrin (Mal-ßCD) on lipid levels and synthesis in Npc1-deficient (Npc1-KO cells) and vehicle CHO cells. (bvsalud.org)
Stable isotope tracer analysis with extracellular addition of D4-deuterated palmitic acid (D4-PA) to Npc1-KO cells increased the synthesis of D4-deuterated LCFAs (D4-LCFAs) and D4-deuterated ECs (D4-ECs) in a Mal-ßCD-dependent manner. (bvsalud.org)
Simultaneous addition of D6-deuterated UEC (D6-UEC) and D4-PA promoted the Mal-ßCD-dependent synthesis of D6-/D4-ECs, consisting of D6-UEC and D4-PA, D4-deuterated stearic acid, or D4-deuterated myristic acid, in Npc1-KO cells. (bvsalud.org)
decreased cholesterol synthesis. (biotech2012.org)
however, cholesterol synthesis was reduced. (biotech2012.org)

All of the BAECs with different cholesterol levels showed little growth on substrates with elasticity below 20 kPa, but increased spreading at higher substrate elasticity. (missouri.edu)
Cholesterol- depleted cells were consistently smaller than control and cho- lesterol-enriched cells regardless of substrate elasticity. (missouri.edu)

At that point, I put my background in biol-ogy to good use and started studying cholesterol and heart disease. (westonaprice.org)

Four wild-type (drug-sensitive) human breast cancer cell lines (MCF-7, T47D,MDA-MB-231, MDA-MB-435) were used to evaluate the influence of acute and chronic exposure to ceramide and ceramide metabolites on MDR1 mRNA,P-gp, and resistance to chemotherapeutic agents. (sphingolipidclub.com)
72 hours) to C8- ceramide (5 μg/ml culture medium), a cell-permeable analog of ceramide, enhanced MDR1 mRNA levels by 3- and 5-fold in T47D and inMDA-MB-435 cells, respectively, but did not affect MCF-7 cells.D-erythro-sphingosine exposure (5 μg/ml, 72 hr) increased MDR1 mRNA levels in MDA-MB-435 cells by 3.5-fold. (sphingolipidclub.com)
Acute exposure ofMCF-7 and MDA-MB-231 cells to C8-glucosylceramide (10 μg/ml culture medium), a cell-permeable analog of glucosylceramide, increased MDR1 mRNA levels by 2- and 4-fold, respectively. (sphingolipidclub.com)
Exposure of cells to either octanoic acid (C8:0), a C8-ceramide hydrolysis product, or oleic acid (C18:1) did not affect MDR1 expression. (sphingolipidclub.com)
These data are the first evidence that chronic exposure to ceramide and its metabolites enhances expression of the MDR phenotype in cancer cells. (sphingolipidclub.com)
Chronic exposure of rats resulted in increased thyroid follicular cell tumors from sustained perturbation of thyroid hormone homeostasis. (cdc.gov)

However, caution is advised in the use of this substance by humans, since a significant reduction in viability of HepG2 and a marked decrease in cell viability on normal PBMC were verified. (geneticsmr.org)

Tumors in which eosinophilic cells predominate were previously classified as "granular cell" carcinoma but are currently included among CCRCCs in the 2004 World Health Organization classification of renal tumors based on the presence of vasculature and genetic alterations typical of CCRCC. (medscape.com)

Despite emergent evidence that TRPA1 is regulated by the membrane environment, it remains unknown whether this channel localizes in membrane microdomains or whether it interacts with cholesterol. (elifesciences.org)
However, it is currently unknown whether TRPA1 is localized in specific domains of the plasma membrane, or whether cholesterol interacts with specific residues of TRPA1. (elifesciences.org)

Organisms usually contain large numbers of sphingolipid subspecies (for a pathway based compilation, see www.sphingomap.org) and knowledge about the types and amounts is imperative because they influence membrane structure, interactions with the extracellular matrix and neighboring cells, vesicular traffic and the formation of specialized structures such as phagosomes and autophagosomes, as well as participate in intracellular and extracellular signaling. (sphingolipidclub.com)

They also found that, while in normal cells the concentration of cholesterol in the inner layer is low, in cancer cells it's much higher. (analytica-world.com)

Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. (riken.jp)

In the present study we examined the relationship between ceramide, ceramide metabolites and expression of the MDR1 gene in human breast cancer cell lines. (sphingolipidclub.com)
This study aimed to explore the effects of chemerin on the functions of human endothelial progenitor cells (EPCs) and to investigate its role in lipid accumulation in ApoE-knockout (ApoE−/−) mice. (biomedcentral.com)
Direct correlation between resistance to chemotherapy and activation of PKB was shown in several prostate cancer cell lines and in human tumorogenic tissues, and elevation of PKB levels in prostate tumor tissues, is clinically associated with HRPC (Yongde et al. (justia.com)
These IMRCs are differentiated from human embryonic stem cells that are expanded and then harvested. (drugbank.com)

Moreover, we identified two structural motifs in transmembrane segments 2 and 4 involved in mTRPA1-cholesterol interactions that are necessary for normal agonist sensitivity and plasma membrane localization. (elifesciences.org)
Interactions between implanted materials and the surrounding host cells critically affect the fate of bioengineered materials. (missouri.edu)

Up-regulation of the LDLR under lipoprotein-deficient conditions facilitates the increased uptake of extracellular LDL (salvage pathway) to restore cellular cholesterol levels. (abt737.com)
HMGCS is one of a battery of enzymes whose expression is up regulated under sterol-deficient conditions to synthesize cholesterol de novo from Fluorescein TSA precursors to restore cellular cholesterol levels (synthetic pathway). (abt737.com)
SREBP2 regulates the transcription of both the LDLR as well as other enzymes in the cholesterol synthetic pathway, including HMGCS. (abt737.com)

The results of these studies provided further evidence that ABCA2 was a sterol-responsive gene involved in cholesterol homeostasis. (abt737.com)
The involvement of autophagy in the development of diabetes is corroborated by affecting the physiology and role of pancreatic β cells and the homeostasis of glucose. (frontiersin.org)

Compared to vehicle cells, Npc1-KO cells exhibited high level of UEC, and low levels of esterified cholesterols (ECs) and long-chain fatty acids (LCFAs). (bvsalud.org)
The difference in lipid levels between Npc1-KO and CHO cells was largely ameliorated by Mal-ßCD administration. (bvsalud.org)
Moreover, the effects of Mal-ßCD were reproduced in the lysosomes prepared from Npc1-KO cells. (bvsalud.org)

Neither the hsp itselfdissociation of hsp-associated whole-cell vaccines and, in this table does not block the dissolution rate of stroke by 26 events classification of head- e-mail: [email protected] family physician or the trivalent form, which can help to position itself correctly. (themauimiracle.org)

However, multidrug-resistant (MDR) cancer cells may avoid the cytotoxic effects of chemotherapy by glycosylating ceramide. (sphingolipidclub.com)
The horny layer is made up of cells (the corneocyts) ordered like a brick wall (board c) whose cement is made up of polyunsaturated fatty acids, cholesterol and ceramide (major constituent). (cho-nature.com)

The roles of CTSB in autophagy, adipogenesis, and cholesterol absorption in the intestine have also been studied. (biolegend.com)
Dysregulated autophagy in pancreatic β cells due to hyperglycemia, oxidative stress, and inflammation is associated with diabetes and accompanied by dysregulated autophagy in insulin target tissues and the progression of diabetic complications. (frontiersin.org)

We hypothesized that these LE/LY cholesterol-sequestering agents would mimic the cellular physiological effects of ABCA2 in cells and may in fact be modulators of ABCA2 expression. (abt737.com)

It is also used to treat high cholesterol . (rxlist.com)
In people with high cholesterol, eating a low-fat diet that includes amaranth oil or muffins enriched with amaranth doesn't seem to lower cholesterol any better than simply following a low-fat diet. (rxlist.com)

The RAW264.7 macrophage cell line was pre-treated with EM or CAM, and stimulated with lipopolysaccharide (LPS). (tu-dortmund.de)
She joined GE from Life Technologies where she was responsible for the development of PD-Direct Bioprocess custom cell line and cell culture media optimization service programs globally. (cellculturedish.com)
She also led an applications team charged with scientific support of new cell line and media products as well as process development. (cellculturedish.com)
For a cell line that tends towards lactate production, medium optimization can address this issue by switching the metabolic flux towards the TCA cycle. (cellculturedish.com)
Serine-palmitoyl transferase (LCB1 subunit) deficient cells derived from CHO-K1 cell line. (riken.jp)

Caenorhabditis elegans, a cholesterol auxotroph, showed defects in larval development upon cholesterol starvation (CS) in a previous study. (molcells.org)
I have made a scientific study of how cholesterol is or is not involved in heart disease. (westonaprice.org)
99:209 -215.doi:10.1007/s11060 - 010 - 0129 -5 cell carcinoma-a population-based study in with and without cervical cancer in Tbilisi, Georgia. (who.int)

Pancreatic histopathology slides demonstrated that stevia extract did not induce any increase in the number of β-cells. (researchgate.net)

Under lipoprotein-deficient culture conditions we measured increased mRNA levels for ABCA2, LDLR and HMGCS that were repressed upon supplementation of culture medium with exogenous cholesterol [46]. (abt737.com)

Real-time measurement of cell elasticity was performed with atomic force microscopy (AFM) and adhesion was assessed with AFM probes coated with fibronectin (FN). (missouri.edu)
Modelling the role of membrane mechanics in cell adhesion on titanium oxide nanotubes. (ki.si)

The complexity of the sphingolipidome is hard to imagine, but one hopes that, when partnered with other systems biology approaches, the causes and consequences of the complexity will explain how these intriguing compounds are involved in almost every aspect of cell behavior and the malfunctions of many diseases. (sphingolipidclub.com)

Since 1993, Brown, Goldstein, and their colleagues have described the unexpectedly complex machinery by which cells maintain the necessary levels of fats and cholesterol in the face of varying environmental circumstances. (wikipedia.org)

Anatomy24

Organisms9

Diseases23

Chemicals and Drugs139

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Psychiatry and Psychology1

Phenomena and Processes42

Technology, Industry, Agriculture16

Information Science3

Health Care4

Wonhwa Cho3

20211

Metabolism12

Proteins4

Luciferase1

Membranes4

Apoptosis7

Total cholesterol8

Biomarkers1

Molecule6

Statin drugs2

Assay3

Lipoprotein cholesterol1

Intracellular cholesterol3

Genes6

Inhibition1

Serum4

Efflux8

Receptors3

Protein5

Lipids3

Triglycerides2

10.10162

Concentrations1

Renal cell carc8

Membrane cholesterol2

U18666A3

Endothelial cells1

Alzheimer's Disease1

Vascular smooth musc1

Tissue2

Amino acid1

Synthesis5

Substrate2

Biol1

Exposure6

Viability1

Tumors1

Interacts2

Extracellular matrix1

Concentration of cholesterol1

Mammalian1

Human4

Interactions2

Pathway3

Homeostasis2

NPC13

Stroke1

Ceramide2

Autophagy2

Physiological1

High cholesterol2

Line5

Study3

Pancreatic1

Supplementation1

Adhesion2

Compounds1

Fats1