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  • Chinese
  • To this end, the ion exchange chromatography recovery of CHO-HBsAg from a recombinant Chinese hamster ovary cell line was shown to increase from about 55 to 80% by the addition of 1% poly(ethylene glycol) (PEG 10,000) to the mobile phase. (diva-portal.org)
  • Incubation of adriamycin resistant Chinese hamster lung cells with low levels of N-ethylmaleimide (NEM) results in a major increase in the cellular accumulation of drug. (semanticscholar.org)
  • Hyperthermia, adriamycin transport, and cytotoxicity in drug-sensitive and -resistant Chinese hamster ovary cells. (semanticscholar.org)
  • Involvement of plasma membrane lipid structural order in adriamycin resistance in Chinese hamster lung cells. (semanticscholar.org)
  • recombinant
  • Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for recombinant protein production. (springer.com)
  • However, by conventional random integration strategy, development of a high-expressing and stable recombinant CHO cell line has always been a difficult task due to the heterogenic insertion and its caused requirement of multiple rounds of selection. (springer.com)
  • In this study, we investigated three sites with potential high transcriptional activities: C12orf35, HPRT, and GRIK1, to determine the possible transcriptional hot spots in CHO cells, and further construct a reliable site-specific integration strategy to develop recombinant cell lines efficiently. (springer.com)
  • Our results demonstrated the feasibility of C12orf35 as the target site for exogenous gene integration, and strongly suggested that C12orf35 targeted integration mediated by CRISPR/Cas9 is a reliable strategy for the rapid development of recombinant CHO cell lines. (springer.com)
  • proliferation
  • It is the first-in-class of a kind of drug called a "HER dimerization inhibitor" - it inhibits the dimerization of HER2 with other HER receptors, which prevents them from signalling in ways that promote cell growth and proliferation. (wikipedia.org)
  • resistance
  • Zeocin and other related chemicals in the bleomycin family of compounds are primarily used in molecular biology as an antibiotic, especially for the selection of eukaryotic cell lines when used in conjunction with vectors containing a selectable marker for zeocin resistance. (wikipedia.org)
  • Multiple independent complementation groups of Chinese hamster ovary (CHO) cells have been isolated, and this gene restored UV resistance to cells of complementation group 4. (wikipedia.org)
  • human
  • Moving to the University of Texas Health Science Center in Dallas, now the UT Southwestern Medical Center, Brown and colleague Joseph L. Goldstein researched cholesterol metabolism and discovered that human cells have low-density lipoprotein (LDL) receptors that extract cholesterol from the bloodstream. (wikipedia.org)
  • protein
  • Bves is a highly conserved, transmembrane protein that is involved in cell adhesion, cell motility, and most recently has been shown to play a role in vesicular transport. (wikipedia.org)
  • Bves interacts with GEFT, a protein that modulates Rho GTPases, Rac1 and Cdc42, which are important for cell motility through modulation of the actin cytoskeleton. (wikipedia.org)
  • In cholesterol-depleted cells, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi apparatus. (wikipedia.org)
  • Regulation
  • Gene regulation gives the cell control over structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism. (wikipedia.org)
  • Gene regulation may also serve as a substrate for evolutionary change, since control of the timing, location, and amount of gene expression can have a profound effect on the functions (actions) of the gene in a cell or in a multicellular organism. (wikipedia.org)
  • cascades
  • Taken together, these data demonstrate that Bves modulates Rho GTPase signaling cascades through interaction with GEFT to affect cell movement and morphology. (wikipedia.org)
  • productivity
  • Site-specific integration of transgenes into CHO hot spots is an ideal strategy to overcome these challenges since it can generate isogenic cell lines with consistent productivity and stability. (springer.com)
  • biology
  • To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. (thermofisher.com)
  • adhesion
  • Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. (wikipedia.org)
  • Grossly, cell motility and cell adhesion are impaired. (wikipedia.org)
  • migration
  • VAMP3 is important for recycling of integrins during cell migration and is also necessary for exocytosis of transferrin. (wikipedia.org)
  • Thus, Bves is important for VAMP3-mediated vesicular transport underlying cell migration and transferrin recycling. (wikipedia.org)
  • Type
  • Glycogen storage disease type II, also called Pompe disease, is an autosomal recessive metabolic disorder which damages muscle and nerve cells throughout the body. (wikipedia.org)
  • cellular
  • The results show that only the hydroxylated derivatives (NMHE and 9-hydroxyellipticinium), which covalently bind to nucleic acids, increase the cellular sensitivity to ionizing radiations, whereas the non-hydroxylated derivative, 2N-methyl-ellipticinium, has no influence on cell radiosensitivity. (biomedsearch.com)
  • cholesterol
  • Since 1993, Drs. Brown, Goldstein, and their colleagues have described the unexpectedly complex machinery by which cells maintain the necessary levels of fats and cholesterol in the face of varying environmental circumstances. (wikipedia.org)
  • sensitive
  • The main effect is observed in the case of exponentially growing cells which are also more sensitive to the drug alone than plateau phase cells. (biomedsearch.com)
  • Thermal radiosensitization in heat- and radiation-sensitive mutants of CHO cells. (biomedsearch.com)
  • Cells with disabling mutations in ERCC4 are more sensitive than normal to particular DNA damaging agents, including ultraviolet radiation and to chemicals that cause crosslinking between DNA strands. (wikipedia.org)
  • metastatic
  • 1999) Rapid induction of cytokine and E-selectin expression in the liver in response to metastatic tumor cells. (imbc-med.ru)
  • As of 2017, pembrolizumab is used via intravenous infusion to treat inoperable or metastatic melanoma, metastatic non-small cell lung cancer (NSCLC) in certain situations, as a second-line treatment for head and neck squamous cell carcinoma (HNSCC), after platinum-based chemotherapy, and for the treatment of adult and pediatric patients with refractory classic Hodgkin's lymphoma (cHL). (wikipedia.org)
  • calcium
  • Effects of hormones and cytokines on stimulation of adenylate cyclase and intracellular calcium concentration in human and canine periodontal ligament cells. (dentisty.org)
  • NADK is also reportedly stimulated by calcium/calmodulin binding in certain cell types, such as neutrophils. (wikipedia.org)
  • Human
  • Evaluation of cytotoxicity in Human noncancer cells indicated that the compounds 3i and 3m were practically ineffective in quiescent peripheral blood lymphocytes, and may have a selective antiproliferative activity against cancer cells. (deepdyve.com)
  • NIH/3T3 and human hepatoma (HepG2) cells were transfected with expression vectors for PPARs and retinoid × receptors, and PPAR responsive element reporter vector. (biomedcentral.com)
  • Dexamethasone enhances the effects of parathyroid hormone (PTH) on human periodontal ligament (PDL) cells in vitro . (dentisty.org)
  • Many viruses including herpes simplex virus (HSV), human immunodeficiency virus (HIV) and human T-lymphotropic virus (HTLV) have been shown to instigate the formation of these junctions between the infected ("donor") and uninfected ("target") cell to allow cell-to-cell transmission. (wikipedia.org)
  • Pembrolizumab is an immunoglobulin G4, with a variable region against the human programmed cell death 1 receptor, a humanized mouse monoclonal [228-L-proline(H10-S>P)]γ4 heavy chain (134-218') disulfide and a humanized mouse monoclonal κ light chain dimer (226-226:229-229)-bisdisulfide. (wikipedia.org)
  • cancer cells
  • It blocks a protective mechanism of cancer cells, and allows the immune system to destroy those cancer cells. (wikipedia.org)
  • Thionicotinamide and other nicotinamide analogs are potential inhibitors of NADK, and studies show that treatment of colon cancer cells with thionicotinamide suppresses the cytosolic NADPH pool to increase oxidative stress and synergizes with chemotherapy. (wikipedia.org)
  • When cancer cells are deficient in ATM the cells are "addicted" to DNA-PKcs, important in the alternative DNA repair pathway for double-strand breaks, non-homologous end joining (NHEJ). (wikipedia.org)
  • novel
  • This project represents an opportunity to harness the novel techniques of synthetic cell bioengineering to establish a UK platform for the optimisation and manufacture of biologics that is robust, flexible/adaptable yet more affordable with the impact of making currently prohibitively expensive therapeutics more widely available and expanding the market into animal health. (ed.ac.uk)
  • signals
  • Sodium-hydrogen antiporters, such as NHEDC2, convert the proton motive force established by the respiratory chain or the F1F0 mitochondrial ATPase into sodium gradients that drive other energy-requiring processes, transduce environmental signals into cell responses, or function in drug efflux. (wikipedia.org)
  • Bacteria
  • Carbapenems are a class of beta-lactam antibiotics that are capable of killing most bacteria by inhibiting the synthesis of one of their cell wall layers. (wikipedia.org)
  • Archives
  • Eun Ju Chang, Won Jung Lee, Sung Hee Cho and Sang Won Choi, Archives of Pharmacal Research, August 2003, Volume 26, Issue 8, pages 620-630, doi:10.1007/BF02976711 Identification of galloylated propelargonidins and procyanidins in buckwheat grain and quantification of rutin and flavanols from homostylous hybrids originating from F. esculentum × F. homotropicum. (wikipedia.org)
  • high
  • Microfluidic high-throughput single-cell assays, next generation sequencing, and data mining based on cell properties and public databases. (umich.edu)
  • lines
  • The modification of isoCA-4 by introduction of difluoromethoxy group at the para-position (3i) and substitution of the two protons of the linker by two fluorine atoms (3m), produced the most active compounds in the series, with IC50 values of 0.15-2.2 nM (3i) and 0.1-2 nM (3m) respectively, against a panel of six cancer cell lines. (deepdyve.com)
  • role
  • The primary role of bacterioruberin in the cell is to protect against DNA damage incurred by UV light. (wikipedia.org)
  • target
  • Abstract: Fabrication of Solid Oxide Fuel Cells (SOFCs) electrolytes by Electrophoretic Deposition (EPD) was the target of our study. (scientific.net)
  • It is able to protect the cell from reactive oxygen species produced from exposure to UV by acting as a target. (wikipedia.org)
  • Levels
  • Potassium levels are not at equilibrium with the environment, so H. salinarum expresses multiple active transporters which pump potassium into the cell. (wikipedia.org)
  • virus
  • As viral synapses allow the virus to spread directly from cell to cell, they also provide a means by which the virus can escape neutralising antibody. (wikipedia.org)
  • important
  • Phosphatidylinositol (3,4)-bisphosphate (PtdIns(3,4)P2) is a minor phospholipid component of cell membranes, yet an important second messenger. (wikipedia.org)
  • thought
  • Viral synapses are thought to explain how cell-to-cell transfer can operate in the HIV infection even when there is a low number of viral particles and a relatively low number of CD4 receptors. (wikipedia.org)
  • Elevated DNA-PKcs is thought to be "beneficial to the tumor cells", though it would be at the expense of the patient. (wikipedia.org)
  • treatment
  • When Dr. Que demanded improvement in the treatment of political prisoners, he was incarcerated in a five-by-six foot cell without sanitary facilities for two months. (wikipedia.org)