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  • enzymatic
  • Here, we describe a method for purifying histidine-tagged pyrophosphokinase enzymes and utilizing thin layer chromatography of radiolabelled substrates and products to assay for the enzymatic activity in vitro . (jove.com)
  • The red cells were washed in buffered saline at 4 o C and lysed and the enzymatic assays were carried out according to standard methods (4,5). (scielo.br)
  • protein
  • A thermal shift assay quantifies the change in thermal denaturation temperature of a protein under varying conditions. (wikipedia.org)
  • Each method has its strengths and weaknesses but they all struggle with intrinsically disordered proteins without any clearly defined tertiary structure as the essence of a thermal shift assay is measuring the temperature at which a protein goes from well-defined structure to disorder. (wikipedia.org)
  • Biuret test Bradford protein assay Van Noorden, R. (wikipedia.org)
  • Here, we demonstrate manufacturable wafer-scale mitochondrial functional assay lab-on-a-chip devices, which require mitochondrial protein quantities three orders of magnitude less than current assays, integrated onto 4′′ standard silicon wafer with new fabrication processes and materials. (rsc.org)
  • Isolate the DNA bound protein by EMSA: the DNA bound protein, migrated in acrylamide gel, is isolated by autoradiography as per an Electrophoretic mobility shift assay (EMSA) protocol. (wikipedia.org)
  • enzyme
  • The enzyme activity assay is broadly applicable to any kinase, nucleotide cyclase, or phosphor-transfer reaction whose mechanism includes nucleotide triphosphate hydrolysis. (jove.com)
  • In the present study the enzyme activity as well as its affinity were determined under nearly physiological conditions regarding osmolarity (290 mOs) and ionic strength (I: 0:188), 3 mM MgCl 2 , pH 7.34, at 37 o C. The activity assay was performed with NADP and glucose-6-phosphate (G6P) in excess. (scielo.br)
  • describes
  • Quantitative Biological and Clinical Mass Spectrometry uses examples where development has not been immediately successful but where unforeseen problems have arisen and describes the strategies used to solve these. (wiley.com)
  • specific
  • Solution assays have proved that the specific activity of ccNiR decreases with the reduction potential of the electronic carriers and ammonium is always the main product of nitrite reduction. (pubmedcentralcanada.ca)
  • presence
  • In this work the kinetic behaviour of ccNiR has been evaluated in a systematic manner using two different spectrophotometric assays carried out in the presence of different redox mediators and a direct electrochemical approach. (pubmedcentralcanada.ca)
  • potential
  • The ATPase assays can therefore have the potential for determining whether a compound acts as a transporter substrate and/or inhibitor. (wikipedia.org)
  • procedures
  • It presents a detailed discussion on how to optimize measurement parameters for a candidate reference quantitative analysis, including calibration procedures, sensitivity, reproducibility, speed of assay and compliance with regulatory authorities. (wiley.com)
  • analysis
  • Quantitative Biological and Clinical Mass Spectrometry appeals to those newly exposed to the use of combined chromatography and mass spectrometry for analysis of biological material and to scientists experienced in automated clinical analysis using immunoassays or who are new to mass spectrometry. (wiley.com)
  • among
  • Conformational changes and coactivator recruitment by novel ligands for estrogen receptor-alpha and estrogen receptor-beta: correlations with biological characterand distinct differences among SRC coactivator family members. (patentgenius.com)
  • large
  • By combining the power of random-sequence selection with pooled sequencing, the SAAB imprint assay makes possible simultaneous screening of a large number of binding site mutants. (wikipedia.org)