Characterization pectin lyase lyases. Medical search. Frequent questions

Pectinases such as pectate lyases degrade pectin and are conducive to higher ethanol yields for these pectin-rich biomasses. (issuu.com)
An analysis of the genomic sequence of P. amylolyticus identified two additional pectate lyases. (issuu.com)
It is likely that the lyases will require calcium ions, prefer an alkaline pH, and have a broad substrate range similar to the two previously characterized pectate lyases in this system. (issuu.com)
Overexpression and Characterization of Two Pectate Lyases from Paenibacillus amylolyticus Rebecca Marie Gardner, CURO Research Assistant Dr. Joy Doran Peterson, Microbiology, Franklin College of Arts and Sciences Cellulosic ethanol is an alternative fuel which has the potential to be cheaper than corn-based ethanol and cleaner than petroleum. (issuu.com)

Alkaline pectate lyase has developmental prospects in the textile, pulp, paper, and food industries. (springeropen.com)

In this study, tailored-made citrus pectin-derived compounds were produced through controlled enzymatic and/or chemical modifications of commercial citrus pectin with different degrees of methylesterification (DM) and similar average molecular weight (M W ). In the first treatment, degradation of the citrus pectin (CP) materials by endo -polygalacturonase (EPG) yielded pectins with average M w 's (between 2 and 60 kDa). (megazyme.com)
Of the three tail spikes, two have protein domains associated with the pectate lyase family of proteins (Tsp2) and P22 tail spike family (Tsp3) with the prospect that these enable Salmonella O antigen degradation. (biomedcentral.com)
These modules incorporate protein families involved in the degradation of cellulose, hemicelluloses, and pectins, structural components of the cellulosome, and additional families with potential functions in plant biomass degradation. (biomedcentral.com)

Pectin lyases are the only known pectinases capable of degrading highly esterified pectins (like those found in fruits) into small molecules via β-elimination mechanism without producing methanol (which is toxic), in contrast with the combination of PG and PE, which are normally found in commercial products. (wikipedia.org)
Complete pectin catabolism requires synergy between multiple pectinases with different substrate specificities (Kamijo et al. (springeropen.com)
Two pectinases found in P. amylolyticus, PelA and PelB have previously been characterized and demonstrate activity on a broad range of pectin, singlehandedly doing the work of multiple pectinases. (issuu.com)

pectin, a plant polysaccharide in the cell wall that provides structure. (issuu.com)

The enzyme activity of PGLA-rep4, measured at 235 nm with 0.2% apple pectin as the substrate, was 554.0 U/mL, and the specific enzyme activity after purification using a nickel column was 822.9 U/mg. (springeropen.com)

Therefore, the characterization of the endo-1,4-beta-xylanase, encoded by um06350.1, is a further step towards the biotechnological application of U . maydis and its enzymes. (biomedcentral.com)

Pectin lyase (EC 4.2.2.10), also known as pectolyase, is a naturally occurring pectinase, a type of enzyme that degrades pectin. (wikipedia.org)
Pectin lyase catalyzes the following process: Eliminative cleavage of (1→4)-α-D-alacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-α,-D-galact-4-enuronosyl groups at their non-reducing ends This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. (wikipedia.org)
The systematic name of this enzyme class is (1→4)-6-O-methyl-α-D-galacturonan lyase. (wikipedia.org)

The first report on retting of sunn hemp (Crotalaria juncea) by pectin lyase produced by Aspergillus flavus MTCC 7589 was published in 2008 but this aspect of pectin lyases needs to be extensively investigated further. (wikipedia.org)

After approximately 20 ns of molecular dynamics simulation, the structure of the pectin lyase PGLA-rep4 tended to be stable. (springeropen.com)
2021 ). At present, based on the pectin molecular domain-connection method, it is believed that the galacturonic acid-containing unit is the "smooth region," while the part containing rhamnogalacturonan I and II is called the "hairy region" (Adetunji et al. (springeropen.com)
Production and molecular characterization of tailored citrus pectin-derived compounds. (megazyme.com)

The optimum pH and temperature of the modified pectin lyase (PGLA-rep4) were 11.0 and 70 °C, respectively. (springeropen.com)
Pectate lyase assays will then be used to identify optimum temperature, pH, etc. (issuu.com)

Diverse pectin-derived compounds were successfully generated for further studies exploring their functionality. (megazyme.com)

Application of mass spectrometry to determine the activity and specificity of pectin lyase A". Carbohydr. (wikipedia.org)
In this study, we selected BacPelA, the pectin lyase with the highest expression activity from Bacillus clausii , modified and expressed in Escherichia coli BL21(DE3). (springeropen.com)

Pectin is a heteropolysaccharide that represents a major component of the middle lamella of plant cell walls, while lignin is a strongly cross-linked polymer of various aromatic compounds. (biomedcentral.com)

Characterization of CNC physiochemical properties, especially its surface chemistry, is essential for the CNC-based materials development. (auburn.edu)

Pectin lyase catalyzes the following process: Eliminative cleavage of (1→4)-α-D-alacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-α,-D-galact-4-enuronosyl groups at their non-reducing ends This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. (wikipedia.org)
A group of carbon-oxygen lyases. (lookformedical.com)

Pectate lyases (PELs) play an important role in the infection process of plant pathogens and also have a commercial significance in industrial applications. (biomedcentral.com)
We cloned a new pectate lyase gene ( Pcpel2 ) from Phytophthora capsici . (biomedcentral.com)

The production of a large-scale soluble recombinant PEL would allow not only a more detailed structural and functional characterization of this enzyme but also may have important applications in the food industry. (biomedcentral.com)
Thus, to aid in the functional characterization of these two proteins and identify the biological processes in which these proteins operate, we used immunoprecipitation coupled with mass spectrometry (IP-MS) to identify proteins interacting with AtNHR2A and AtNHR2B and to generate their interactome network. (frontiersin.org)

In this study, we obtained a highly efficient alginate-degrading strain, Vibrio pelagius WXL662, with 11 alginate lyases (VpAly-I to -XI) and further elucidated its molecular mechanism of alginate degradation. (nih.gov)

It is produced commercially for the food industry from fungi and used to destroy residual fruit starch, known as pectin, in wine and cider. (wikipedia.org)