ESBLIsolatesAmpCGenesExtended-spectrumPhenotypicPlasmidsHydrolyzeAntimicrobialBacteriaESBLsAntibioticsMetalloStrainsKlebsiellaPrevalenceCarbapenemasesESCHERICHIA COLIEnzymesCephalosporinsAntibioticEspectroGeneReceptorsMechanismsNitrocefinUrinaryMolecular classesProteinsClassEnzymeFoundThailandStructure
ESBL12
- The infections caused by the Extended Spectrum Beta Lactamase (ESBL) enzymes-producing E. coli hinder antibiotic treatment. (nih.gov)
- In the current study prevalence and characterization of extended spectrum beta-lactamase(ESBL) production of Escherichia coli was detected from broilers of West Bengal, India. (ijcmas.com)
- Aim: To determine the prevalence of UPEC, rate of MDR strains, to characterize phenotypic screening and genotypic characterization of the ESBL producing genes among uropathogenic E. coli. (journalcra.com)
- The extended-spectrum β-lactamase (ESBL)-producing bacteria have been isolated at increasing frequency worldwide. (scielo.br)
- O isolamento de bactérias produtoras de beta-lactamases de espectro expandido (ESBL) está aumentando no mundo todo. (scielo.br)
- Extended-spectrum beta-lactamase (ESBL) and AmpC enzymes are important AMR mechanisms that erode the efficacy of important antibiotics. (eijppr.com)
- For direct detection of extended-spectrum beta-lactamase (ESBL) producers, new real-time PCR assay will be performed. (imtm.cz)
- Prompt detection of extended-spectrum β-lactamases (ESBL) and carbapenemase-producing enterobacteriaceae is crucial for infection prevention and control strategies. (ac.ir)
- 3. Khanfar HS, Bindayna KM, Senok AC, Botta GA. Extended spectrum beta-lactamases (ESBL) in Escherichia coli and Klebsiella pneumoniae: trends in the hospital and community settings. (ac.ir)
- 4. Kohlenberg A, Schwab F, Rüden H. Wide dissemination of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella spp. (ac.ir)
- 5. Haghighatpanah M, Mozaffari Nejad AS, Mojtahedi A, Amirmozafari N, Zeighami H. Detection of extended-spectrum beta-lactamase (ESBL) and plasmid-borne blaCTX-M and blaTEM genes among clinical strains of Escherichia coli isolated from patients in the north of Iran. (ac.ir)
- Four clinical isolates of multidrug-resistant extended-spectrum β-lactamase (ESBL)-producing uropathogenic E. coli were included in the study. (biomedcentral.com)
Isolates13
- E.coli isolates were serotyped and also screened for extended spectrum beta-lactamase by double disc diffusion assay and by PCR detection of bla CTX-M, bla SHV, and bla TEM genes. (ijcmas.com)
- This study was aimed at detecting the occurrence of metallo β-lactamase (MBL) and AmpC β-lactamase genes from environmental isolates of Escherichia coli, Klebsiella species and Pseudomonas aeruginosa. (edu.ng)
- Metallo-β-lactamase was phenotypically detected in 22 E. coli isolates, 22 P. aeruginosa isolates and 18 isolates of Klebsiella species. (edu.ng)
- Metallo-β-lactamase encoding genes (particularly the blaIMP-1 genes and blaIMP-2 genes) was detected by PCR in 12 (54.6 %) isolates of E. coli, 15 (83.3 %) isolates of Klebsiella species and 16 (72.7 %) isolates of P. aeruginosa. (edu.ng)
- Despite the recent global spread of CTX-M -lacta- -lactamase genes located on mobile genetic elements such mases in Escherichia coli isolates from community-ac- as plasmids or transposons. (cdc.gov)
- During January--June 2010, three Enterobacteriaceae isolates carrying a newly described resistance mechanism, the New Delhi metallo-beta-lactamase (NDM-1) ( 1 ), were identified from three U.S. states at the CDC antimicrobial susceptibility laboratory. (cdc.gov)
- CDC asks that carbapenem-resistant isolates from patients who have received medical care within 6 months in India or Pakistan be forwarded through state public health laboratories to CDC for further characterization. (cdc.gov)
- The ability of extended spectrum β-lactamases (ESBLs) production in E. coli isolates was detected using the combined disc diffusion method. (biomedcentral.com)
- This study determined the prevalence of MDR A. baumannii isolates from intensive care units in a large tertiary-care hospital in Ismailia, Egypt, and the occurrence of different beta-lactamases in these isolates. (who.int)
- Among the isolates, 6 isolates were carbapenemase producers, 2 were AmpC beta-lactamase producers and no isolates were metallo-beta- lactamase producers. (who.int)
- All isolates of enterobacteria will be tested for the presence of blaTEM, blaSHV and blaCTX-M genes (responsible for extended-spectrum beta-lactamase production) using PCR method and the eventual occurrence of genes enabling carbapenemases production (KPC, SME, IMI, VIM, IMP, OXA enzyme types etc.) will be assessed. (imtm.cz)
- In this study, we provide the molecular characterization and epidemiology for 12 NDM-1 positive clinical isolates. (who.int)
- Isolates should be submitted to the regional laboratories of the ARLN for further characterization (potential novel carbapenemase). (cdc.gov)
AmpC6
- Metallo-β-lactamase and AmpC β-lactamase production was phenotypically carried out using ceftazidime-imipenem antagonism test (CIAT), cefoxitin-cloxacillin double disk synergy test (CC-DDST), modified Hodges test, and inhibition based assay. (edu.ng)
- Despite from a hospital environment, belonged to the TEM or SHV possessing the gene encoding cephalosporinase, ampC ( 1 ), -lactamase family, and were produced by Klebsiella spp. (cdc.gov)
- The mechanisms include extended-spectrum β-lactamases (ESBLs), AmpC β-lactamases, and carbapenemases of molecular classes A and B. (asm.org)
- e subtipagem molecular de plasmídeos carreando genes de beta-lactamases de espectro estendido e AmpC / Phenotypic and genotypic characterization of strains of Salmonella spp. (bvsalud.org)
- AmpC mais aucun isolat ne produisait de métallo-bêta-lactamases. (who.int)
- High prevalence of extended-spectrum beta-lactamases and plasmid-mediated AmpC beta-lactamases in Enterobacteriaceae isolated from long-term care facilities in Korea. (ac.ir)
Genes4
- Analysis of two highly expressed genes from Chlorella virus PBCV-1: Protein characterization and the DNA sequences of the major capsid protein gene and the early/late 33-kDa protein gene. (uml.edu)
- ESBLs are typically inhibitor-susceptible β-lactamases that hydrolyze penicillins, cephalosporins, and aztreonam and are encoded by mobile genes. (asm.org)
- Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)
- 1 The ease of β-lactamase encoding genes ( bla NDM-1 ) dissemination has become apparent with the worldwide detection of NDM-1 producers. (who.int)
Extended-spectrum5
- TEM-1, TEM-2, 2-year prospective study, we documented the prevalence and SHV-1) and extended-spectrum -lactamases (ESBLs) of extended-spectrum -lactamases (ESBLs) in E. coli that (e.g. (cdc.gov)
- OBJECTIVES: Escherichia coli producing CTX-M-type extended-spectrum beta-lactamases (ESBLs) are spreading worldwide. (who.int)
- The main mechanisms of resistance to these antimicrobial classes are the production of extended spectrum beta-lactamases and carbapenemases. (usp.br)
- The production of extended spectrum β-lactamases (ESBLs) is a primary β-lactam resistance mechanism in Gram-negative bacteria. (biomedcentral.com)
- Prevalence and characterization of extended spectrum beta-lactamase-producing Enterobacter cloacae strains in Algeria. (ac.ir)
Phenotypic4
- Molecular and Phenotypic Characterization of a Highly Evolved Type 2 Vaccine-Derived Poliovirus Isolated from Seawater in Brazil, 2014. (cdc.gov)
- The Etest MBL (bioMérieux, Marcy l'Etoile, France) was used for the phenotypic detection of metallo-β-lactamases. (who.int)
- Positive on a phenotypic test for carbapenemase production (e.g., metallo-β-lactamase test, modified Hodge test, Carba NP, Carbapenem Inactivation Method [CIM], or modified CIM). (cdc.gov)
- An isolate that tests positive on MHT but negative PCR for KPC, NDM, OXA-48, VIM and IMP should have additional characterization performed with another phenotypic test for carbapenemase such as mCIM. (cdc.gov)
Plasmids1
- Another probe which comprised the whole of the OXA-2 beta-lactamase gene as well as segments of DNA on either side of the gene, was found to hybridise with a number of resistance plasmids. (bl.uk)
Hydrolyze4
- The beta-lactamase could hydrolyze both penicillin antibiotics including ampicillin, benzylpenicillin, and carbenicillin as well as cephalosporin antibiotics including nitrocefin, cephalothin, cephaloridine, and cefoperazone. (lu.se)
- CTX-M -lactamases has reached a critical level in Cam- are cefotaximases that usually hydrolyze cefotaxime rather bodia, which highlights the need for study of their spread in than ceftazidime. (cdc.gov)
- Resistance against β-lactam agents can occur via (i) mutation or expression of alternative penicillin-binding proteins (PBPs) as the drug target, (ii) downregulation of porins to reduce the bacterial permeability against β-lactams, (iii) over-expression of efflux systems which are membrane transport proteins to export drug substrates and (iv) production of β-lactamases that hydrolyze the β-lactam amide [ 6 ]. (biomedcentral.com)
- The BETA-LACTAMASES hydrolyze the beta lactam ring, accounting for BETA-LACTAM RESISTANCE of infective bacteria. (lookformedical.com)
Antimicrobial1
- These gram-negative bacteria can produce β-lactamases (Wickens and Wade, 2005) which creates antimicrobial resistance in them. (arccjournals.com)
Bacteria5
- Metallo-beta-lactamases are important enzymes because they are involved in the breakdown of antibiotics by antibiotic-resistant bacteria. (wikipedia.org)
- So, the present study was undertaken to investigate the prevalence of these two bacteria from cloaca and environment of ducks from different districts of West Bengal, followed by detailed characterization and antibiogram. (arccjournals.com)
- Characterization of different strategies used by uropathogenic bacteria to weaken or modulate the host immune response is one research area of major interest. (oru.se)
- Bacteriological and molecular biology tools were used for the isolation and characterization of bacteria. (eijppr.com)
- The aim of the thesis is the detection and characterization of bacteria producing beta-lactamases isolated from patients with leukemia. (imtm.cz)
ESBLs1
- A total of 34 strains carried ESBLs, all of which ed -lactamases of Kluyvera sp. (cdc.gov)
Antibiotics4
- P. aeruginosa also produces numerous antibiotic-inactivating enzymes such as β-lactamases, which target and break down β-lactam antibiotics. (uoguelph.ca)
- These results show that PA2915 and PA0832 possess low level β-lactamase activity but do not provide any significant resistance against β-lactam antibiotics in P. aeruginosa. (uoguelph.ca)
- These zinc-β-lactamases (ZBLs) inactivate β-lactam antibiotics through hydrolysis of the β-lactam ring. (wikipedia.org)
- Carbapenemases are a versatile group of �-lactamases that are characterised by their resistance to virtually all �-lactam antibiotics including cephalosporins and carbapenems, complicating therapy and limiting treatment options. (omicsonline.org)
Metallo8
- The metallo-β-lactamase (MBL) superfamily constitutes a group of proteins found in all domains of life that share a characteristic αββα fold with the ability to bind transition metal ions. (wikipedia.org)
- New Delhi metallo-beta-lactamase González, JM (2021). (wikipedia.org)
- This is the first report of NDM-1 in the United States, and the first report of metallo-beta-lactamase carriage among Enterobacteriaceae in the United States. (cdc.gov)
- Characterization of a new metallo-β-lactamase gene, bla NDM-1 , and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. (cdc.gov)
- Resistance to carbapenem is mostly conferred by metallo β-lactamase (IMP, VIM and NDM) and carbapenem hydrolyzing class D β-lactamase (OXA-48 like). (omicsonline.org)
- The most common carbapenemase in Enterobacteriaceae belongs to class A carbapenemase (KPC), class B metallo �-lactamases (IMP, VIM, NDM) and class D oxacillinase (OXA-48 like) [ 2 , 3 ]. (omicsonline.org)
- The discovery of a novel carbapenemase, the New Delhi metallo-β-lactamase-1 (NDM-1), generated much global alarm. (who.int)
- Metallo-β-lactamase testing (e.g. (cdc.gov)
Strains2
Klebsiella1
- RÉSUMÉ L'émergence et la propagation rapide des souches de Klebsiella pneumoniae résistantes aux antibiotiques et porteuses du gène blaKPC codant la production de carbapénèmases ont compliqué la prise en charge des infections des patients. (who.int)
Prevalence2
- Although many studies have investigated the prevalence of Salmonella infection in farms ( 15 - 17 ), genomic characterization of Salmonella infection in slaughterhouses are still lagging in China. (frontiersin.org)
- INTRODUCTION: The treatment of healthcare associated infections caused by enterobacteria represents a growing challenge due to the increasing prevalence of beta-lactam resistance, particularly to third and fourth generations cephalosporins and carbapenems. (usp.br)
Carbapenemases1
- Os mecanismos de resistência mais relevantes a essas classes de antimicrobianos, em enterobactérias, é a produção de betalactamases de espectro ampliado e carbapenemases. (usp.br)
ESCHERICHIA COLI2
Enzymes1
- A more detailed analysis has revealed from comparison of both primary and predicted secondary structure, that the OXA-2 beta-lactamase may be more closely related to class A enzymes. (bl.uk)
Cephalosporins1
- Amino acid sequencing of some beta-lactamases has shown that substitution of only a few amino acids in the bla gene leads to high-level resistance against specific cephalosporins. (lu.se)
Antibiotic1
- His primary research area includes molecular characterization of beta-lactamases that cause bacterial resistance against a broad-spectrum antibiotic. (atlanticbooks.com)
Espectro1
- Caracterização de betalactamases de espectro ampliado e KPC em Enterobacter cloacae. (usp.br)
Gene3
Receptors1
- The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS. (lookformedical.com)
Mechanisms1
- Clavulanic acid and a novel beta-lactamase inhibitor, BRL36148, both interact specifically with the OXA-2 beta-lactamase by branched pathway mechanisms. (bl.uk)
Nitrocefin2
- In addition, the target proteins were biochemically characterized in vitro by detecting β-lactamase activity using the standard chromogenic β-lactam substrate, nitrocefin. (uoguelph.ca)
- β-lactamase characterization was carried out using nitrocefin sticks. (edu.ng)
Urinary1
- Most -lactamases found in E. quired urinary tract infections (CA-UTIs), their dissemina- coli belong to Ambler class A and can be further divided tion has been little studied in developing countries. (cdc.gov)
Molecular classes1
- The resistance mechanism for CPE is attributed to the following Ambler molecular classes of carbapenem-hydrolysing beta-lactamases: class A (KPC), class B (IMP, NDM, VIM), and class D (OXA-48) [ 5 ]. (biomedcentral.com)
Proteins1
- Through proteomic analysis of the P. aeruginosa laboratory strain PAO1, a number of uncharacterized proteins were identified that are predicted to be novel β-lactamases. (uoguelph.ca)
Class3
- no good homologies were observed with any class of beta-lactamase. (bl.uk)
- It confirmed that the enzyme belonged to a class A beta-lactamase which had 99% identity to the ampicillin resistance transposon Tn3 of pBR322. (lu.se)
- Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. (lookformedical.com)
Enzyme3
- Confirmation that the OXA-2 beta-lactamase is a serine enzyme has come from the DNA sequence and the interaction with mechanism based inactivators. (bl.uk)
- Characterisation of the physical properties of the OXA-2 enzyme by analytical ultracentrifugation have not only confirmed the dimeric nature of this beta-lactamase but have also shown that this enzyme forms aggregates at high protein concentrations. (bl.uk)
- Probing of the enzyme structure with trypsin, strongly points to the OXA-2 beta-lactamase having a domain structure. (bl.uk)
Found1
- An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. (lookformedical.com)
Thailand1
- Molecular characterization of Nipah virus from Pteropus hypomelanus in Southern Thailand. (cdc.gov)
Structure2
- Overall, this work provides the foundation for future structure function studies of these putative β-lactamases. (uoguelph.ca)
- Molecular cloning, characterization and three-dimensional structure prediction of Lipoxygenase from Finger millet [Eleusine coracana (L.) Gaertn. (molquest.com)