APOJProteinsProteinMolecular chaperoneDrosophilaNeuronsApoEPotentApoptosisGlycoproteinAggregationEndoplasmicCellularIntracellularDynamicsFacilitatesTake part in the assembly processCellsAssemblyPlasmaExpressionMajorFunctionSamplesExtracellular chaperone clusterinGeneMonoclonal AntibodyRecombinantMOLECULAR CHAPERONECytoprotectionAntisense oligonucleotideP10909InhibitsVariantsHSC70ApolipoproteinSubunitsRegulationInteractionsMechanismDegradationBindingCancerStructureActivityAppearsFunctionSourceRoleStudy
APOJ3
- Clusterin (CLU) also known as APOJ in humans. (promegaconnections.com)
- Our previous study demonstrated that after developmental seizures in rats, E-64d exerts a neuroprotective effect on the seizure-induced brain damage by modulating lipid metabolism enzymes, especially ApoE and ApoJ/clusterin. (omicsdi.org)
- ApoJ (clusterin) , also known as testosterone repressed prostate message-2, sulfated glycoprotien-2, and Sp-40 and CLU, functions as a secreted molecular chaperone protein , which may have either an intracellular or extracellular function. (wellnessadvantage.com)
Proteins5
- α2M and clusterin are molecular chaperones involved in extracellular proteostasis, but α2M has a greater affinity for aggregation-prone proteins. (eurekalert.org)
- The ability of neurons to manage the burden of misfolded proteins and to resist their accumulation into insoluble protein deposits depends critically on the functioning of molecular chaperones. (biomedcentral.com)
- A few mammalian extracellular proteins i.e. clusterin, haptoglobin and α2-macroglobulin display chaperone activity by stabilizing secreted misfolded and aggregation-prone proteins such as amyloid-β. (uni-tuebingen.de)
- In protein science , Molecular Chaperones , in our body's cells, are a family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. (wellnessadvantage.com)
- In our body, Molecular Chaperones are able to inhibit the aggregation of partially denatured proteins and refold them. (wellnessadvantage.com)
Protein7
- On this basis, we hypothesized that TBI leads to increases in the brain levels of clusterin protein, and consequently, increased plasma clusterin levels. (nature.com)
- Immunohistochemistry revealed a marked increase in extracellular clusterin protein expression in the perilesional cortex and ipsilateral hippocampus (7d to 1 month post-TBI), and ipsilateral thalamus (14d to 12 months post-TBI). (nature.com)
- We have examined in this study whether or not increased expression of clusterin is able to protect neuronal cells against intracellular protein aggregation and cytotoxicity, characteristics that are strongly implicated in a range of neurodegenerative diseases. (biomedcentral.com)
- We used the amyotrophic lateral sclerosis-associated protein TDP-43 as a primary model to investigate the effects of clusterin on protein aggregation and neurotoxicity in complementary in vitro, neuronal cell and Drosophila systems. (biomedcentral.com)
- This protein is an extracellular chaperone and may also have functions in lipid transport and modulating the immune response. (promegaconnections.com)
- Yan D. et al "Paeoniflorin, a novel heat shock protein-inducing compound" Cell Stress Chaperones. (wellnessadvantage.com)
- An acidic protein found in the NEUROENDOCRINE SYSTEM that functions as a molecular chaperone for PROPROTEIN CONVERTASE 2. (bvsalud.org)
Molecular chaperone2
- Therapeutic potential of the molecular chaperone and matrix metallopro" by M. Elizabeth Fini, Shinwu Jeong et al. (edu.au)
- Previously characterized as a molecular chaperone, CLU is expressed prominently by epithelia at fluid-tissue interfaces and secreted into bodily fluids, where it protects cells and tissues against damaging stress. (edu.au)
Drosophila2
- We further showed that the expression of TDP-43 in transgenic Drosophila neurons induced ER stress and that co-expression of clusterin resulted in a dramatic clearance of mislocalized TDP-43 from motor neuron axons, partially rescued locomotor activity and significantly extended lifespan. (biomedcentral.com)
- We also showed that in Drosophila photoreceptor cells, clusterin co-expression gave ER stress-dependent protection against proteotoxicity arising from both Huntingtin-Q128 and mutant (R406W) human tau. (biomedcentral.com)
Neurons1
- Here we show in a cellular model system and in neurons that Clusterin, an abundant extracellular chaperone, strongly enhances Tau aggregate seeding. (synergy-munich.de)
ApoE1
- ApoE and clusterin cooperatively suppress Abeta levels and deposition: evidence that ApoE regulates extracellular Abeta metabolism in vivo. (jamanetwork.com)
Potent1
- Upon interaction with Tau aggregates, Clusterin stabilizes highly potent, soluble seed species. (synergy-munich.de)
Apoptosis1
- Clusterin (apolipoprotein J) is a highly conserved heterodimeric glycoprotein ( apolipoprotein ), differentially expressed during many severe physiological disturbance states, such as cancer, apoptosis, and various neurological disorder. (wellnessadvantage.com)
Glycoprotein1
- Clusterin is a highly conserved glycoprotein ubiquitously expressed in the brain as well as in peripheral tissues in different species, including rodents and humans 15 . (nature.com)
Aggregation2
- Tau/Clusterin complexes enter recipient cells via endocytosis and compromise the endolysosomal compartment, allowing transfer to the cytosol where they propagate aggregation of endogenous Tau. (synergy-munich.de)
- We have shown that clusterin directly interacts with TDP-43 in vitro and potently inhibits its aggregation, and observed that in ER stressed neuronal cells, clusterin co-localized with TDP-43 and specifically reduced the numbers of cytoplasmic inclusions. (biomedcentral.com)
Endoplasmic1
- It is now widely accepted in the field that the normally secreted chaperone clusterin is redirected to the cytosol during endoplasmic reticulum (ER) stress, although the physiological function(s) of this physical relocation remain unknown. (biomedcentral.com)
Cellular1
- The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model. (synergy-munich.de)
Intracellular2
- We therefore conclude that increased expression of clusterin can provide an important defense against intracellular proteotoxicity under conditions that mimic specific features of neurodegenerative disease. (biomedcentral.com)
- Most chaperones are localised within intracellular compartments, although some are secreted into the extracellular environment. (biomedcentral.com)
Dynamics1
- In the nucleus it behaves as a DNA chaperone, sustains nucleosome dynamics and chromosomal stability, and contributes to telomere maintenance [ 10 ]. (hindawi.com)
Facilitates1
- Subsequently it was demonstrated that the ER-resident chaperone BiP (GRP78) facilitates the translocation of CLU to the cytosol during ER stress in human prostate cancer LNCaP cells [ 16 ]. (biomedcentral.com)
Take part in the assembly process2
- Although they take part in the assembly process, molecular chaperones are not components of the final structures. (reference.md)
- In our body's cells, Molecular Chaperones take part in the assembly process of polypeptides, but are not components of the final structures. (wellnessadvantage.com)
Cells1
- In addition, E-64d treatment regulated mitochondrial pathway activity and inhibited chaperone-mediated autophagy in HT22 cells. (omicsdi.org)
Assembly1
- Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2. (mpg.de)
Plasma2
- Our findings suggest that plasma clusterin is a candidate biomarker for acute TBI diagnosis. (nature.com)
- Prominent amongst such extracellular chaperones is clusterin (CLU), which is present in both plasma and cerebrospinal fluid (CSF). (biomedcentral.com)
Expression1
- Our previous mRNA-seq data revealed a 1.8-2.5 fold increase in clusterin mRNA expression in lesioned brain areas in rats with lateral fluid-percussion injury (FPI)-induced TBI. (nature.com)
Major1
- This graph shows the total number of publications written about "Molecular Chaperones" by people in this website by year, and whether "Molecular Chaperones" was a major or minor topic of these publications. (jefferson.edu)
Function1
- Chaperone Function of Hgh1 in the Biogenesis of Eukaryotic Elongation Factor 2. (mpg.de)
Samples2
- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clusterin (CLU) were tested 20 times on one plate, respectively. (biomatik.com)
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clusterin (CLU) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
Extracellular chaperone clusterin2
Gene3
- Therefore, this study aimed to assess the potential relationship between single nucleotide polymorphisms (SNPs) in the 3' untranslated region or introns of the clusterin gene (CLU) and the susceptibility to developing PEG or PEX. (nih.gov)
- 17. Secreted clusterin gene silencing enhances chemosensitivity of a549 cells to cisplatin through AKT and ERK1/2 pathways in vitro. (nih.gov)
- Clusterin, an extracellular chaperone, is the most highly expressed gene in the seal brain and fourfold higher than in the ferret or any other mammalian brain transcriptome. (uit.no)
Monoclonal Antibody3
- Formalin-fixed, paraffin-embedded human adrenal gland stained with Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4730). (neobiotechnologies.com)
- SDS-PAGE Analysis Purified Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4730). (neobiotechnologies.com)
- therapeutic_agents C1909 therapeutic_agents C C177537 GDC Value Terminology C121778 Sotevtamab A humanized, immunoglobulin (Ig) G2 monoclonal antibody against the secreted form of human clusterin (sCLU) expressed by tumor cells, with potential antineoplastic and anti-metastatic activities. (nih.gov)
Recombinant4
- and characterisation of bacterially expressed recombinant clusterin domains. (edu.au)
- The antibody was raised in rabbits by immunization with the recombinant Rat Clusterin. (biovendor.com)
- Recombinant Rat Clusterin, total 240 AA. (biovendor.com)
- Immunoaffinity chromatography on a column with immobilized recombinant Rat Clusterin. (biovendor.com)
MOLECULAR CHAPERONE1
- Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE . (bvsalud.org)
Cytoprotection1
Antisense oligonucleotide1
P109091
- We believe that clusterin (CLU, UniProtKB P10909 ), a plasma circulating glycoprotein, plays a role in the pathobiology of ATTRwt amyloidosis. (bioinfor.com)
Inhibits1
- BAG3 Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. (neuromics.com)
Variants1
HSC701
- They bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. (neuromics.com)
Apolipoprotein1
- According to Roxana Carare, MD PhD, at the University of Southampton, apolipoprotein J (also called clusterin) is being investigated for its role in chaperoning Amyloid Beta for brain waste clearance along the intramural periarterial pathway (IPAD). (amyloidangiopathy.com)
Subunits1
Regulation1
Interactions1
Mechanism1
- A detailed mechanism of clusterin has not been defined. (biovendor.com)
Degradation2
- 1. Clusterin facilitates COMMD1 and I-kappaB degradation to enhance NF-kappaB activity in prostate cancer cells. (nih.gov)
- Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation (PubMed:21505792). (neobiotechnologies.com)
Binding2
- Vitronectin was successfully quantitated using this approach, but the measurement of clusterin was not reliable because of high inter-well variation of binding. (nih.gov)
- Binding to the known clusterin ligand megalin was also tested with a similar binding affinity seen for both molecules, however the removal of sugars increased the rate at which the molecule bound to and dissociated from megalin. (edu.au)
Cancer2
Structure2
- A primary aim of the work described in this thesis was to examine the relationship between clusterin structure and function. (edu.au)
- Clusterin markedly influences 尾-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis. (neobiotechnologies.com)
Activity1
- The chaperone activity of the deglycosylated molecule was also not different to the wild type molecule. (edu.au)
Appears1
- Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. (neobiotechnologies.com)
Function1
Source1
- clusterin [Source:HGNC Symbol;Acc:HG. (gsea-msigdb.org)
Role1
- Moreover, results comparing CLU oligosaccharide variations between patient and healthy controls are original and provide further evidence for the role of CLU in ATTRwt pathobiology, possibly linked to disease-specific structural features that limit the chaperoning capacity of CLU. (bioinfor.com)