Polymerase Chain ReNucleotide polymorphismConformation Polymorphism AnalysisMutationGeneticNovel polymorphismsAllelesSSCPSNPsGeneSequencingDouble-strandedRFLPOverhangAnalysisSequencesFibrilsHaplotypeProteinsStructuralComplementarySequenceNascentHeterogeneityMethodsVirusStructureExpressionRateHighlyTemplateBaseForms
Polymerase Chain Re2
- The aim of this study was to detect carriers of CVM in Holstein population using Polymerase Chain Reaction- Primer-introduced restriction analysis and Restriction Fragment Length Polymorphism (PCR-PIRA and RFLP) methods. (researchgate.net)
- A single forward primer and two species-specific reverse primers were used to produce a polymerase chain reaction (PCR) fragment of 306 bp and 543 bp for S. bovis and S. haematobium, respectively. (imperial.ac.uk)
Nucleotide polymorphism2
- Possible applications in the distant future may include the ultrasensitive detection of single nucleotide polymorphism. (nanowerk.com)
- This method is based on the extension of a primer that anneals immediately adjacent to the single-nucleotide polymorphism with fluorescent dideoxynucleotide triphosphates (minisequencing), followed by analysis on an ABI Prism 3700 capillary electrophoresis instrument. (ox.ac.uk)
Conformation Polymorphism Analysis1
Mutation4
- SSCP is also widely used in virology to detect variations in different strains of a virus, the idea being that a particular virus particle present in both strains will have undergone changes due to mutation, and that these changes will cause the two particles to assume different conformations and, thus, be differentiable on an SSCP gel. (wikipedia.org)
- 2006. Tumors from rats given 1,2-dimethylhydrazine plus chlorophyllin or indole-3-carbinol contain transcriptional changes in beta-catenin that are independent of beta-catenin mutation status. . (oregonstate.edu)
- In an individual with the sporadic/relapsing form of the disease we have found a mutation comprising a deletion, subsequent frame shift and premature stop codon leading to half normal levels of serum factor H. In one of the three families there is a point mutation in exon 20 causing an arginine to glycine change, which is likely to alter structure and hence function of the factor H protein. (nih.gov)
- METHODS: These methods included clinical diagnosis, mutation detection, haplotype analysis, and functional expression studies using single-channel recordings of mutant AChR transiently transfected into HEK293 cells. (ox.ac.uk)
Genetic1
Novel polymorphisms1
- In this study we systematically screened 3.5 kb of the PSEN1 upstream region and found four novel polymorphisms. (ox.ac.uk)
Alleles1
- the physical properties of the double strands are almost identical for both alleles. (wikipedia.org)
SSCP4
- Single-strand conformation polymorphism (SSCP), or single-strand chain polymorphism, is defined as a conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions. (wikipedia.org)
- The difference in shape between two single-stranded DNA strands with different sequences can cause them to migrate differently through an electrophoresis gel, even though the number of nucleotides is the same, which is, in fact, an application of SSCP. (wikipedia.org)
- SSCP used to be a way to discover new DNA polymorphisms apart from DNA sequencing but is now being supplanted by sequencing techniques on account of efficiency and accuracy. (wikipedia.org)
- PCR-SSCP analyses indicated a higher level of base changes in smokers than in non-smokers. (mcmaster.ca)
SNPs1
- By optimised primer design it was possible to type the 4 relevant single nucleotide polymorphisms (SNPs) in the prnp simultaneously in one multiplex reaction. (biomedcentral.com)
Gene9
- Our results show that one out of 60 bulls tested exhibited polymorphism (G→T) at position 559 in exon 4 of SLC35A3 gene. (researchgate.net)
- This thesis discusses research conducted to examine the hypothesis that this is due to polymorphisms in the region of the IL-6 gene controlling its expression. (ucl.ac.uk)
- Single-strand conformational polymorphism analysis of a PCR-generated fragment from the 5' flanking region of the IL-6 gene (-550 to +61) indicated the existence of polymorphisms. (ucl.ac.uk)
- The other patients showed either deletions of the SMN gene (49/54) or a gene conversion event changing SMN exon 7 into its highly homologous copy (cBCD541, 1/54). (nih.gov)
- Numerous studies have evaluated the relationship between RNASEL gene polymorphisms (rs486907 G>A and rs627928 T>G) and the risk of cancer. (medscimonit.com)
- To explore the role of RNASEL gene polymorphisms in prostate cancer, we carried out the present meta-analysis. (medscimonit.com)
- Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
- Therefore, the demand on genotyping capacities of the polymorphisms of the prion protein gene ( prnp ) relevant to presently known disease associations and EU regulations is steadily increasing. (biomedcentral.com)
- Susceptibility to scrapie is largely controlled by three polymorphic amino acid positions (136, 154, 171) of the ovine prion protein gene ( prnp ) [ 4 ] and reliable genotyping of the corresponding DNA polymorphism is required as a basis for selection decisions. (biomedcentral.com)
Sequencing4
- To confirm this polymorphism, the PCR product was purified using ExoSAP-IT followed by sequencing by Applied Biosystems 3130XL Automated Sequencer using the ABI BigDye Ver 3.1. (researchgate.net)
- Based on the findings of single-cell RNA sequencing (scRNA-seq), viral RNA has been detected within macrophages in lung tissue and bronchoalveolar lavage (BAL) fluid from COVID-19 patients. (news-medical.net)
- Direct sequencing covering the region of exon 2 encoding amino acid positions 136, 154 and 171 is the most accurate method, that enables the typing of additional and detection of hitherto unknown polymorphisms. (biomedcentral.com)
- We considered clusters of isolates with 5 single-nucleotide polymorphisms identified by whole-genome sequencing to indicate recent transmission and clusters of 10 persons to be outbreaks. (cdc.gov)
Double-stranded1
- During the life cycle of HBV, RT utilizes a single-stranded viral genomic RNA as a template to synthesize a hybrid RNA-DNA duplex, and then converts it to double-stranded DNA (ds-DNA). (biomedcentral.com)
RFLP1
- RFLP analysis of the -174 polymorphism in 59 Caucasian S-JCA patients demonstrated that they had a lower C allele frequency than a group of healthy Caucasians (n=72) (0.35 vs 0.53). (ucl.ac.uk)
Overhang3
- The deactivator competitively binds onto the single-stranded overhang at the end of helix H, silencing the sticky ends. (nanowerk.com)
- This binding leaves an overhang handle for the activator to bind and later remove the deactivator via three-stranded branch migration. (nanowerk.com)
- The formation of G-quadruplex DNA is easily conceivable in DNA sequences that are present as single strands in cells, such as the telomeric overhang. (hindawi.com)
Analysis3
- We developed and used new single-molecule fluorescence spectroscopic and fluorescence lifetime imaging methods, combined with deep learning for image analysis. (biorxiv.org)
- In this initial study, we combine single-molecule Förster resonance energy transfer (FRET) spectroscopy, fluorescence lifetime imaging (FLIM), and image analysis using deep learning (codes are available at https://github.com/hoisunglab/FNet ) to interrogate several steps during the aggregation process of the 42-residue Aβ peptide (Aβ42), including dimerization, formation of stable oligomers, and fibril elongation. (biorxiv.org)
- Functional polymorphisms in CYTOCHROME P450 monooxygenases that metabolize nicotine have now been defined and it should soon be possible to identify fast nicotine metabolizers by DNA analysis. (ox.ac.uk)
Sequences1
- Nevertheless, we have to keep in mind that these G-quadruplex-forming sequences although transiently single-stranded are a priori not as easily accessible as the telomeric sequences, due to the presence of the transcription machinery [ 28 ]. (hindawi.com)
Fibrils2
- Polymorphism in the structure of fibrils formed by aggregation suggests the existence of many different assembly pathways and therefore a heterogeneous ensemble of soluble oligomers. (biorxiv.org)
- Aggregation to form fibrils is also highly heterogeneous in terms of the number of strands in a fibril and the elongation speed and conformation of fibrils. (biorxiv.org)
Haplotype1
Proteins1
Structural1
Complementary2
- The device is made by introducing complementary singlestranded overhangs at the two arms of the DNA four-way junction. (nanowerk.com)
- G-rich DNA strands are naturally involved in duplex-DNA architecture through association with their complementary C-rich DNA strands by the canonical Watson-Crick pairing [ 1 - 3 ]. (hindawi.com)
Sequence2
- After denaturation, single-stranded DNA undergoes a characteristic 3-dimensional folding and may assume a unique conformational state based on its DNA sequence. (wikipedia.org)
- This may lead to a single molecule sensor of minute sequence differences of a target DNA. (nanowerk.com)
Nascent1
- Active forms of NRTIs incorporate into the elongating DNA strand, terminate the elongation of the nascent DNA strand, and prevent additional dNTPs from being incorporated. (biomedcentral.com)
Heterogeneity2
- They learned that even a single base pair difference in the sticky end can be easily detected and single molecular heterogeneity is small enough that even at the single nanometronome level it should be possible to distinguish one base pair difference. (nanowerk.com)
- Single-molecule spectroscopy can be an effective tool to probe heterogeneity because molecular species can be observed one at a time, so individual oligomers can be detected without separation. (biorxiv.org)
Methods1
- Length Polymorphism (PCR-PIRA and RF LP) methods. (researchgate.net)
Virus2
- SARS-CoV-2 is a single-stranded, enveloped, positive-sense ribonucleic acid (RNA) virus that belongs to the family Coronaviridae. (news-medical.net)
- Hepatitis C virus (HCV), a member of the Flaviviridae family, is a single-stranded enveloped RNA virus that infects approximately 170 million people worldwide. (xiahepublishing.com)
Structure1
- Indeed, given that four guanine residues can self-associate in a planar arrangement through a Hoogsteen-type hydrogen-bonding network [ 4 ] to form a structure called G-quartet, G-rich strands can adopt a peculiar three-dimensional arrangement called G-quadruplex DNA [ 5 - 9 ] resulting from the stacking of several contiguous G-quartets (Figure 1 ). (hindawi.com)
Expression1
- Following stimulation with lipopolysaccharide or interleukin-1, luciferase expression from the C(A8T12) construct did not change. (ucl.ac.uk)
Rate1
- We named it 'single molecule nanometrome' because the ticking rate of this ten nanometers sized device is tunable, and the device was observed one molecule at a time. (nanowerk.com)
Highly1
- This RD-PCR proved highly sensitive, detecting a single larval stage and as little as 0.78 ng of genomic DNA (gDNA) from an adult schistosome, providing a cost-effective, rapid and robust molecular tool for high-throughput screening of S. haematobium and S. bovis populations. (imperial.ac.uk)
Template2
- aRNA is RNA that is transcribed from the coding, rather than the template, strand of DNA. (genomicglossaries.com)
- In our previous work using single-molecule FRET and MD simulation, we have shown that the monomer of Aβ42 is almost completely disordered with no structured regions that could possibly be a template for fibril formation 11 . (biorxiv.org)
Base1
- 'Single Molecule Nanometronome' in the Feb. 10, 2006 online edition of Nano Letters , Taekjip Ha and his colleagues describe that the device displays clearly distinguishable responses even with a single base pair difference. (nanowerk.com)
Forms1
- We propose that the pH-dependent changes of the protonation states of glutamate/aspartate residues of APRs initiate switching between the amyloid and the folded, monomeric forms of the hormones. (nature.com)