LuxAB was used to screen the substrate scopes of three prokaryotic oxidoreductases: an alcohol dehydrogenase (Pseudomonas putida), a choline oxidase variant (Arthrobacter chlorophenolicus) and a carboxylic acid reductase (Mycobacterium marinum). (tuni.fi)
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. (cancer-genetics.org)
Oxidation3
ALDH3A2 catalyzes the oxidation of long-chain aliphatic aldehydes into fatty acids. (wikipedia.org)
Furthermore, the dual role of LuxAB as sensor and monooxygenase, emitting bioluminescence through the oxidation of aldehydes to the corresponding carboxylates, promises implementation in artificial enzyme cascades for the synthesis of carboxylic acids. (tuni.fi)
The encoded enzyme is responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. (wikipedia.org)
Sjögren-Larsson syndrome (SLS) is diagnosed by demonstrating the enzyme deficiency or by mutation analysis of the FALDH gene. (medscape.com)
Sjögren-Larsson syndrome (SLS) is due to deficient activity of fatty aldehyde dehydrogenase (FALDH), an enzyme required to oxidize fatty alcohol to fatty acid. (medscape.com)
Fatty3
In contrast, free fatty aldehyde levels are not elevated. (medscape.com)
Accumulation of long-chain fatty alcohols and modification of macromolecules by an excess of fatty aldehydes are thought to be the pathophysiologic mechanisms causing the manifestations of Sjögren-Larsson syndrome. (medscape.com)
Aliphatic3
It is known to act on a variety of both saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length, as well as dihydrophytal, a 20-carbon branched chain aldehyde. (wikipedia.org)
A simple high-throughput assay utilized the bacterial luciferase-previously reported to only accept aliphatic long-chain aldehydes-to detect structurally diverse aldehydes, including aromatic and monoterpene aldehydes. (tuni.fi)
The AOR/AdhA pathway also converts exogenously added aliphatic and aromatic carboxylic acids to the corresponding alcohol using glucose, pyruvate, and/or hydrogen as the source of reductant. (brenda-enzymes.info)
Enzymatic2
In this work, the LuxAB biosensor system from Photorhabdus luminescens was implemented in Escherichia coli to monitor the enzymatic production of aldehydes from primary alcohols and carboxylic acid substrates. (tuni.fi)
The enzymatic requirements for EtOH to inhibit RA biosynthesis were studied using inhibitors of the middle-chain alcohol dehydrogenases (ADH), 4-methylpyrazole (4MP), or the short-chain dehydrogenase/reductases, carbenoxolone (CBX) and chloral hydrate (CH). Late blastula stage embryos were treated with EtOH alone or in combination with 4MP (a,b), CH (c,d) or CBX (e,f). (xenbase.org)
FALDH2
To date, more than 70 different FALDH gene mutations have been found in patients with Sjögren-Larsson syndrome. (medscape.com)
The gene encoding FALDH is called the ALDH3A2 gene (or ALDH10 ) and has been mapped to the Sjögren-Larsson syndrome locus on band 17p11.2. (medscape.com)
Ferredoxin1
The engineered strain converts glucose to ethanol via acetate and acetaldehyde, catalyzed by the host-encoded aldehyde ferredoxin oxidoreductase AOR and heterologously expressed AdhA, in an energy-conserving, redox-balanced pathway. (brenda-enzymes.info)
ALDH1A22
In the embryo , RA production by RALDH2 (ALDH1A2), the main retinaldehyde dehydrogenase expressed at that stage, is inhibited by ethanol exposure. (xenbase.org)
Aldehyde dehydrogenases 1A2 (ALDH1A2) was thought to be one of potential candidates. (ijbs.com)
Superfamily1
ALDH3A2 belongs to the aldehyde dehydrogenase superfamily and is a membrane-associated protein typically containing 485 residues. (wikipedia.org)
Chromosome1
This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. (cancer-genetics.org)
The application of genetically encoded biosensors enables the detection of small molecules in living cells and has facilitated the characterization of enzymes, their directed evolution and the engineering of (natural) metabolic pathways. (tuni.fi)
However, we previously demonstrated that the grisemycin (grm) cluster contained cryptic dehydratase and epimerase genes by heterologous expression of this biosynthetic gene cluster in Streptomyces lividans and proposed that two genes (grmH and grmL) with unknown functions catalyze dehydration and epimerization reactions. (hokudai.ac.jp)
Acetaldehyde1
Acetaldehyde inhibits retinoic acid biosynthesis to mediate alcohol teratogenicity. (xenbase.org)
Inhibition2
Alcohol consumption during pregnancy induces Fetal Alcohol Spectrum Disorder (FASD), which has been proposed to arise from competitive inhibition of retinoic acid (RA) biosynthesis. (xenbase.org)
The biosynthetic gene clusters of these linaridins lack obvious candidate genes for the dehydratase and epimerase required to introduce dehydrobutyrine and D-amino acid residues, respectively. (hokudai.ac.jp)
Ethanol1
deletion of the hypoxanthine phosphoribosyltransferase gene in ethanol tolerant strain adhE*(EA), carrrying mutation P704L/H734R in the alcohol dehydrogenase gene, and deletion of lactate dehydrogenase (ldh) to redirect carbon flux towards ethanol reults in a strain producing 30% more ethanol than wild type on minimal medium. (brenda-enzymes.info)
ALDH3A21
The alternative transcript differs by an additional exon and anchors differently to the endoplasmic reticulum vs. the peroxisome Mutations and deletions within the ALDH3A2 gene have been widely associated with the autosomal recessive Sjögren-Larsson syndrome, an autosomal recessive neurocutaneous disease. (wikipedia.org)
Deletions1
72), including amino acid substitutions, small and large contiguous gene deletions, insertions, and splicing errors in this gene. (medscape.com)
Mutations1
Beyond mutations, the neurologic and other phenotypic characteristics may result from unidentified epigenetic/environmental factors, gene modifiers, or other mechanisms. (medscape.com)
Protein4
The 30-kDa membrane-bound c-type cytochrome protein of mitochondria that functions as an electron donor to CYTOCHROME C GROUP in the mitochondrial and bacterial RESPIRATORY CHAIN. (lookformedical.com)
A bacterial protein from Pseudomonas, Bordetella, or Alcaligenes which operates as an electron transfer unit associated with the cytochrome chain. (lookformedical.com)
construction of a synthetic pathway for bioalcohol production at 70°C by insertion of the gene for alcohol dehydrogenase AdhA into the archaeon Pyrococcus furiosus. (brenda-enzymes.info)
Expression6
The effect on RA signaling was determined by monitoring the expression level of the known RA-regulated genes, HoxA1 and HoxB1 during early gastrula stages. (xenbase.org)
The changes in the expression of several RA-regulated genes was determined after AcAL (5 µM), EtOH (0.5%), DEAB (100 µM), or RA (1 µM). (xenbase.org)
The expression level of the RA-regulated genes HoxA1 (a), HoxB1 (b), HoxB4 (c), Cyp26A1 (d), Dhrs3 (e), and Rdh10 (f) by qPCR. (xenbase.org)
Liang H, Wu C, Deng Y, Zhu L, Zhang J, Gan W, Tang C, Xu R. Aldehyde Dehydrogenases 1A2 Expression and Distribution are Potentially Associated with Neuron Death in Spinal Cord of Tg(SOD1*G93A)1Gur Mice. (ijbs.com)
Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancer-genetics.org)
Because we showed that integration neither affects the levels of viral genes, nor those of virally disrupted human genes, a genome-wide screen was performed to identify human genes which expression is influenced by viral integration and have clinical relevance. (cancer-genetics.org)
Production1
alpha-ketoisovalerate decarboxylase Kivd from Lactococcus lactis combined with alcohol dehydrogenase Adh3 from Zymomonas mobilis are the optimum candidates for 3-methyl-1-butanol production in Corynebacterium glutamicum. (brenda-enzymes.info)
Important1
These findings advance the bio-based detection, preparation and transformation of industrially important aldehydes in living cells. (tuni.fi)