• molecule
  • Here, we determined crystal structures of ADIPOR2 bound to a FFA molecule and demonstrate that ADIPOR2 possesses an intrinsic basal ceramidase activity enhanced by adiponectin. (pubmedcentralcanada.ca)
  • lysosomal
  • In contrast, a number of weakly basic B-13 analogues have been shown to accumulate in the acidic compartments of living cells and to efficiently inhibit lysosomal acid ceramidase. (hindawi.com)
  • cellular
  • However, introduction of weakly basic groups at the ω -position of the fatty acid moiety of B-13 led to a significant reduction of potency towards acid ceramidase from cellular extracts. (hindawi.com)
  • protein
  • Here, we show that expression of ceramidase facilitates the dissolution of incompletely formed and inappropriately located elements of rhabdomeric membranes in ninaE(I17) mutants lacking the G protein receptor Rh1 in R1-R6 photoreceptor cells. (umassmed.edu)
  • mice
  • UT Southwestern researchers showed that introducing ceramidase in diabetic mice returned their insulin sensitivity to normal. (wikipedia.org)
  • activity
  • Because we found that ADIPOR1 also possesses an intrinsic ceramidase activity, we suspect that the two distinct structures may represent key steps of the ADIPORs enzymatic activity. (pubmedcentralcanada.ca)
  • The ceramidase activity is low, however, and further studies will be required to fully characterize ADIPORs enzymatic parameters and their substrate specificity. (pubmedcentralcanada.ca)
  • Recently, the pleiotropic actions of adiponectin were linked to the ceramide signaling pathway as ADIPOR1 and ADIPOR2 activation was shown to lower ceramide levels via the activation of a ceramidase activity measured in crude cell lysates 1 . (pubmedcentralcanada.ca)
  • expression
  • Ceramidase expression facilitates the endocytic turnover of Rh1. (umassmed.edu)
  • Although ceramidase expression aids the removal of internalized rhodopsin, it does not affect the turnover of Rh1 in photoreceptors maintained in dark, where Rh1 is not activated and thus has a slower turnover and a long half-life. (umassmed.edu)
  • Therefore, the phenotypic consequence of ceramidase expression in photoreceptors is caused by facilitation of endocytosis. (umassmed.edu)